LOCUS       DAYYDS010000001        95905 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000001 DAYYDS010000000
VERSION     DAYYDS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 95905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.001"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..562
                     /locus_tag="ACHA02_000001"
     CDS             <1..562
                     /locus_tag="ACHA02_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8668393.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="ACHA02_000002"
     CDS             762..1076
                     /locus_tag="ACHA02_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8668394.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="ACHA02_000003"
     CDS             1097..1474
                     /locus_tag="ACHA02_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8668395.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="ACHA02_000004"
     CDS             1484..2257
                     /locus_tag="ACHA02_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668396.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="ACHA02_000005"
     CDS             2279..3682
                     /locus_tag="ACHA02_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8668397.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /gene="mobT"
                     /locus_tag="ACHA02_000006"
     CDS             3864..5048
                     /gene="mobT"
                     /locus_tag="ACHA02_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8668398.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="ACHA02_000007"
     CDS             5045..5335
                     /locus_tag="ACHA02_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668399.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="ACHA02_000008"
     CDS             5332..5553
                     /locus_tag="ACHA02_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668400.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="ACHA02_000009"
     CDS             5595..6374
                     /locus_tag="ACHA02_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFM8668401.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6435..6935
                     /locus_tag="ACHA02_000010"
     CDS             6435..6935
                     /locus_tag="ACHA02_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8668402.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6991..7629
                     /locus_tag="ACHA02_000011"
     CDS             6991..7629
                     /locus_tag="ACHA02_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFM8668403.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="ACHA02_000012"
     CDS             7701..8096
                     /locus_tag="ACHA02_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8668404.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="ACHA02_000013"
     CDS             8080..10533
                     /locus_tag="ACHA02_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8668405.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="ACHA02_000014"
     CDS             10530..12557
                     /locus_tag="ACHA02_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8668406.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLIQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="ACHA02_000015"
     CDS             12554..13576
                     /locus_tag="ACHA02_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM8668407.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="ACHA02_000016"
     CDS             13593..14522
                     /locus_tag="ACHA02_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8668408.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14836..14883
                     /locus_tag="ACHA02_000017"
                     /pseudo
     CDS             14836..14883
                     /locus_tag="ACHA02_000017"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14899..15699
                     /locus_tag="ACHA02_000018"
                     /pseudo
     CDS             14899..15699
                     /locus_tag="ACHA02_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            15937..16173
                     /locus_tag="ACHA02_000019"
     CDS             15937..16173
                     /locus_tag="ACHA02_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668409.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16180..18132
                     /locus_tag="ACHA02_000020"
     CDS             16180..18132
                     /locus_tag="ACHA02_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8668410.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="ACHA02_000021"
     CDS             complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="ACHA02_000021"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8668411.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            18976..20103
                     /locus_tag="ACHA02_000022"
                     /pseudo
     CDS             18976..20103
                     /locus_tag="ACHA02_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            20201..20389
                     /locus_tag="ACHA02_000023"
     CDS             20201..20389
                     /locus_tag="ACHA02_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM8668412.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20447..20800)
                     /locus_tag="ACHA02_000024"
     CDS             complement(20447..20800)
                     /locus_tag="ACHA02_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8668413.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21331..21753
                     /locus_tag="ACHA02_000025"
     CDS             21331..21753
                     /locus_tag="ACHA02_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8668414.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21750..21980
                     /locus_tag="ACHA02_000026"
     CDS             21750..21980
                     /locus_tag="ACHA02_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8668415.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22478..22678
                     /locus_tag="ACHA02_000027"
     CDS             22478..22678
                     /locus_tag="ACHA02_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFM8668416.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22705..23898
                     /locus_tag="ACHA02_000028"
     CDS             22705..23898
                     /locus_tag="ACHA02_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8668417.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="ACHA02_000029"
     CDS             complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="ACHA02_000029"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8668418.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25590..26513)
                     /gene="coaA"
                     /locus_tag="ACHA02_000030"
     CDS             complement(25590..26513)
                     /gene="coaA"
                     /locus_tag="ACHA02_000030"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8668419.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26709..27314
                     /locus_tag="ACHA02_000031"
     CDS             26709..27314
                     /locus_tag="ACHA02_000031"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8668420.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27622..28638
                     /locus_tag="ACHA02_000032"
     CDS             27622..28638
                     /locus_tag="ACHA02_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8668421.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28779..30080
                     /locus_tag="ACHA02_000033"
     CDS             28779..30080
                     /locus_tag="ACHA02_000033"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8668422.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30126..30788
                     /gene="deoC"
                     /locus_tag="ACHA02_000034"
     CDS             30126..30788
                     /gene="deoC"
                     /locus_tag="ACHA02_000034"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8668423.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30809..31207
                     /locus_tag="ACHA02_000035"
     CDS             30809..31207
                     /locus_tag="ACHA02_000035"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8668424.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31273..32355
                     /locus_tag="ACHA02_000036"
     CDS             31273..32355
                     /locus_tag="ACHA02_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8668425.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32567..34129
                     /locus_tag="ACHA02_000037"
     CDS             32567..34129
                     /locus_tag="ACHA02_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8668426.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34122..35255
                     /locus_tag="ACHA02_000038"
     CDS             34122..35255
                     /locus_tag="ACHA02_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8668427.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35255..36211
                     /locus_tag="ACHA02_000039"
     CDS             35255..36211
                     /locus_tag="ACHA02_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8668428.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36310..37476
                     /gene="deoB"
                     /locus_tag="ACHA02_000040"
     CDS             36310..37476
                     /gene="deoB"
                     /locus_tag="ACHA02_000040"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8668429.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37500..38318
                     /locus_tag="ACHA02_000041"
     CDS             37500..38318
                     /locus_tag="ACHA02_000041"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8668430.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38335..39042
                     /gene="deoD"
                     /locus_tag="ACHA02_000042"
     CDS             38335..39042
                     /gene="deoD"
                     /locus_tag="ACHA02_000042"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8668431.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="ACHA02_000043"
     CDS             complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="ACHA02_000043"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8668432.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(40198..40779)
                     /locus_tag="ACHA02_000044"
     CDS             complement(40198..40779)
                     /locus_tag="ACHA02_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8668433.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="ACHA02_000045"
     CDS             complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="ACHA02_000045"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8668434.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41914..42327
                     /gene="rpsL"
                     /locus_tag="ACHA02_000046"
     CDS             41914..42327
                     /gene="rpsL"
                     /locus_tag="ACHA02_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8668435.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42411..42881
                     /gene="rpsG"
                     /locus_tag="ACHA02_000047"
     CDS             42411..42881
                     /gene="rpsG"
                     /locus_tag="ACHA02_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8668436.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42975..45062
                     /gene="fusA"
                     /locus_tag="ACHA02_000048"
     CDS             42975..45062
                     /gene="fusA"
                     /locus_tag="ACHA02_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8668437.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            45234..46421
                     /gene="tuf"
                     /locus_tag="ACHA02_000049"
     CDS             45234..46421
                     /gene="tuf"
                     /locus_tag="ACHA02_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8668438.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46828..47136
                     /gene="rpsJ"
                     /locus_tag="ACHA02_000050"
     CDS             46828..47136
                     /gene="rpsJ"
                     /locus_tag="ACHA02_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8668439.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            47167..47796
                     /gene="rplC"
                     /locus_tag="ACHA02_000051"
     CDS             47167..47796
                     /gene="rplC"
                     /locus_tag="ACHA02_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8668440.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47824..48447
                     /gene="rplD"
                     /locus_tag="ACHA02_000052"
     CDS             47824..48447
                     /gene="rplD"
                     /locus_tag="ACHA02_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8668441.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            48447..48737
                     /gene="rplW"
                     /locus_tag="ACHA02_000053"
     CDS             48447..48737
                     /gene="rplW"
                     /locus_tag="ACHA02_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8668442.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48776..49609
                     /gene="rplB"
                     /locus_tag="ACHA02_000054"
     CDS             48776..49609
                     /gene="rplB"
                     /locus_tag="ACHA02_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8668443.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49652..49930
                     /gene="rpsS"
                     /locus_tag="ACHA02_000055"
     CDS             49652..49930
                     /gene="rpsS"
                     /locus_tag="ACHA02_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8668444.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49953..50300
                     /gene="rplV"
                     /locus_tag="ACHA02_000056"
     CDS             49953..50300
                     /gene="rplV"
                     /locus_tag="ACHA02_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8668445.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            50314..50970
                     /gene="rpsC"
                     /locus_tag="ACHA02_000057"
     CDS             50314..50970
                     /gene="rpsC"
                     /locus_tag="ACHA02_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8668446.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50973..51407
                     /gene="rplP"
                     /locus_tag="ACHA02_000058"
     CDS             50973..51407
                     /gene="rplP"
                     /locus_tag="ACHA02_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8668447.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            51397..51585
                     /gene="rpmC"
                     /locus_tag="ACHA02_000059"
     CDS             51397..51585
                     /gene="rpmC"
                     /locus_tag="ACHA02_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8668448.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51610..51876
                     /gene="rpsQ"
                     /locus_tag="ACHA02_000060"
     CDS             51610..51876
                     /gene="rpsQ"
                     /locus_tag="ACHA02_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8668449.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51934..52302
                     /gene="rplN"
                     /locus_tag="ACHA02_000061"
     CDS             51934..52302
                     /gene="rplN"
                     /locus_tag="ACHA02_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8668450.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            52339..52647
                     /gene="rplX"
                     /locus_tag="ACHA02_000062"
     CDS             52339..52647
                     /gene="rplX"
                     /locus_tag="ACHA02_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8668451.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52675..53214
                     /gene="rplE"
                     /locus_tag="ACHA02_000063"
     CDS             52675..53214
                     /gene="rplE"
                     /locus_tag="ACHA02_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8668452.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            53233..53418
                     /locus_tag="ACHA02_000064"
     CDS             53233..53418
                     /locus_tag="ACHA02_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8668453.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            53454..53852
                     /gene="rpsH"
                     /locus_tag="ACHA02_000065"
     CDS             53454..53852
                     /gene="rpsH"
                     /locus_tag="ACHA02_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8668454.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53884..54420
                     /gene="rplF"
                     /locus_tag="ACHA02_000066"
     CDS             53884..54420
                     /gene="rplF"
                     /locus_tag="ACHA02_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8668455.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54581..54937
                     /gene="rplR"
                     /locus_tag="ACHA02_000067"
     CDS             54581..54937
                     /gene="rplR"
                     /locus_tag="ACHA02_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8668456.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54958..55458
                     /gene="rpsE"
                     /locus_tag="ACHA02_000068"
     CDS             54958..55458
                     /gene="rpsE"
                     /locus_tag="ACHA02_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8668457.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            55473..55652
                     /gene="rpmD"
                     /locus_tag="ACHA02_000069"
     CDS             55473..55652
                     /gene="rpmD"
                     /locus_tag="ACHA02_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8668458.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55696..56136
                     /gene="rplO"
                     /locus_tag="ACHA02_000070"
     CDS             55696..56136
                     /gene="rplO"
                     /locus_tag="ACHA02_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8668459.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            56136..57431
                     /gene="secY"
                     /locus_tag="ACHA02_000071"
     CDS             56136..57431
                     /gene="secY"
                     /locus_tag="ACHA02_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8668460.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            57493..58140
                     /locus_tag="ACHA02_000072"
     CDS             57493..58140
                     /locus_tag="ACHA02_000072"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8668461.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            58331..58549
                     /gene="infA"
                     /locus_tag="ACHA02_000073"
     CDS             58331..58549
                     /gene="infA"
                     /locus_tag="ACHA02_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8668462.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58582..58698
                     /gene="rpmJ"
                     /locus_tag="ACHA02_000074"
     CDS             58582..58698
                     /gene="rpmJ"
                     /locus_tag="ACHA02_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8668463.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58716..59081
                     /gene="rpsM"
                     /locus_tag="ACHA02_000075"
     CDS             58716..59081
                     /gene="rpsM"
                     /locus_tag="ACHA02_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8668464.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            59109..59498
                     /gene="rpsK"
                     /locus_tag="ACHA02_000076"
     CDS             59109..59498
                     /gene="rpsK"
                     /locus_tag="ACHA02_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8668465.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59578..60516
                     /locus_tag="ACHA02_000077"
     CDS             59578..60516
                     /locus_tag="ACHA02_000077"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8668466.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60544..60924
                     /gene="rplQ"
                     /locus_tag="ACHA02_000078"
     CDS             60544..60924
                     /gene="rplQ"
                     /locus_tag="ACHA02_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8668467.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            61196..62035
                     /locus_tag="ACHA02_000079"
     CDS             61196..62035
                     /locus_tag="ACHA02_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8668468.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            62011..62880
                     /locus_tag="ACHA02_000080"
     CDS             62011..62880
                     /locus_tag="ACHA02_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8668469.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62870..63667
                     /locus_tag="ACHA02_000081"
     CDS             62870..63667
                     /locus_tag="ACHA02_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8668470.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63870..64616
                     /gene="truA"
                     /locus_tag="ACHA02_000082"
     CDS             63870..64616
                     /gene="truA"
                     /locus_tag="ACHA02_000082"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8668471.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64949..67618)
                     /locus_tag="ACHA02_000083"
     CDS             complement(64949..67618)
                     /locus_tag="ACHA02_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8668472.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(67856..68044)
                     /gene="rpmB"
                     /locus_tag="ACHA02_000084"
     CDS             complement(67856..68044)
                     /gene="rpmB"
                     /locus_tag="ACHA02_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8668473.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            68314..68676
                     /locus_tag="ACHA02_000085"
     CDS             68314..68676
                     /locus_tag="ACHA02_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8668474.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            68728..70410
                     /locus_tag="ACHA02_000086"
     CDS             68728..70410
                     /locus_tag="ACHA02_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8668475.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            70527..72563
                     /gene="recG"
                     /locus_tag="ACHA02_000087"
     CDS             70527..72563
                     /gene="recG"
                     /locus_tag="ACHA02_000087"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8668476.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72612..73613
                     /gene="plsX"
                     /locus_tag="ACHA02_000088"
     CDS             72612..73613
                     /gene="plsX"
                     /locus_tag="ACHA02_000088"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8668477.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            73735..73977
                     /gene="acpP"
                     /locus_tag="ACHA02_000089"
     CDS             73735..73977
                     /gene="acpP"
                     /locus_tag="ACHA02_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8668478.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            74245..75249
                     /locus_tag="ACHA02_000090"
     CDS             74245..75249
                     /locus_tag="ACHA02_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8668479.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            75250..76194
                     /locus_tag="ACHA02_000091"
     CDS             75250..76194
                     /locus_tag="ACHA02_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8668480.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            76194..77156
                     /gene="opp4B"
                     /locus_tag="ACHA02_000092"
     CDS             76194..77156
                     /gene="opp4B"
                     /locus_tag="ACHA02_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8668481.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            77183..78097
                     /locus_tag="ACHA02_000093"
     CDS             77183..78097
                     /locus_tag="ACHA02_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8668482.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            78123..79904
                     /locus_tag="ACHA02_000094"
     CDS             78123..79904
                     /locus_tag="ACHA02_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8668483.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            80252..80938
                     /gene="rnc"
                     /locus_tag="ACHA02_000095"
     CDS             80252..80938
                     /gene="rnc"
                     /locus_tag="ACHA02_000095"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8668484.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            80958..84539
                     /gene="smc"
                     /locus_tag="ACHA02_000096"
     CDS             80958..84539
                     /gene="smc"
                     /locus_tag="ACHA02_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8668485.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            84548..85357
                     /locus_tag="ACHA02_000097"
     CDS             84548..85357
                     /locus_tag="ACHA02_000097"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8668486.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            85368..86366
                     /gene="ftsY"
                     /locus_tag="ACHA02_000098"
     CDS             85368..86366
                     /gene="ftsY"
                     /locus_tag="ACHA02_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8668487.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            86553..88820
                     /gene="gshAB"
                     /locus_tag="ACHA02_000099"
     CDS             86553..88820
                     /gene="gshAB"
                     /locus_tag="ACHA02_000099"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8668488.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(88909..89361)
                     /locus_tag="ACHA02_000100"
     CDS             complement(88909..89361)
                     /locus_tag="ACHA02_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8668489.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            89511..90461
                     /locus_tag="ACHA02_000101"
     CDS             89511..90461
                     /locus_tag="ACHA02_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8668490.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            90454..91413
                     /locus_tag="ACHA02_000102"
     CDS             90454..91413
                     /locus_tag="ACHA02_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8668491.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            91410..92165
                     /locus_tag="ACHA02_000103"
     CDS             91410..92165
                     /locus_tag="ACHA02_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8668492.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            92188..93141
                     /locus_tag="ACHA02_000104"
     CDS             92188..93141
                     /locus_tag="ACHA02_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8668493.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(93785..94081)
                     /locus_tag="ACHA02_000105"
     CDS             complement(93785..94081)
                     /locus_tag="ACHA02_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8668494.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            94436..94792
                     /locus_tag="ACHA02_000106"
     CDS             94436..94792
                     /locus_tag="ACHA02_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8668495.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            94802..95701
                     /locus_tag="ACHA02_000107"
     CDS             94802..95701
                     /locus_tag="ACHA02_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8668496.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        31585 a        17029 c        20163 g        27128 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattga
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt
    15721 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
    15781 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
    15841 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
    15901 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
    15961 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
    16021 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
    16081 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
    16141 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
    16201 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
    16261 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
    16321 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
    16381 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
    16441 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
    16501 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
    16561 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
    16621 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
    16681 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
    16741 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
    16801 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
    16861 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
    16921 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
    16981 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
    17041 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
    17101 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
    17161 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
    17221 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
    17281 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
    17341 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
    17401 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
    17461 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
    17521 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
    17581 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
    17641 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
    17701 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
    17761 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
    17821 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
    17881 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
    17941 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
    18001 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
    18061 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
    18121 tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg
    18181 ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa
    18241 tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat
    18301 tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg
    18361 atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata
    18421 aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca
    18481 caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag
    18541 gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta
    18601 tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc
    18661 actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta
    18721 actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg
    18781 ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa
    18841 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
    18901 tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca
    18961 aaactgaact acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat
    19021 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
    19081 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
    19141 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
    19201 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
    19261 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
    19321 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
    19381 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
    19441 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
    19501 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
    19561 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
    19621 gggatacgct atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc
    19681 tgttttaagt acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt
    19741 actcctattg tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat
    19801 cttagtttta aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc
    19861 aaatattgtg caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga
    19921 gaaattcctg ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg
    19981 cttagtgttt gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc
    20041 cagacccgtc gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact
    20101 tagtgcgttt tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt
    20161 taatatagat ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat
    20221 attgtgtcct atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa
    20281 aaatttccct ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat
    20341 gaatataata acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg
    20401 aaactctcat cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat
    20461 ttcttgcttc attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa
    20521 agctatccat gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag
    20581 gtaagaaaaa ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg
    20641 tacttgggtg ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca
    20701 ttgttcccac ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac
    20761 caacggctct aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat
    20821 tttactgttc ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt
    20881 tcctatagaa cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg
    20941 ctgattttat ttacctatac aactttataa aatttagtca tactcctaga aagtagataa
    21001 aattggcgac ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct
    21061 cttaattaag ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa
    21121 tgtggttttg cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct
    21181 gtttatgtgc ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca
    21241 acaaatcccc cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact
    21301 ccctttcacg acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata
    21361 gaaaatcagt ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc
    21421 aaacatctag acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt
    21481 gtcaactctt tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt
    21541 ggattaagaa tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag
    21601 aaaaaacgga acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg
    21661 gagttactaa atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg
    21721 attaaagaat tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc
    21781 ccctcattgt aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt
    21841 atcgagggta tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc
    21901 atatggttat tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt
    21961 tatcgttcga aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc
    22021 ttcccgattt gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga
    22081 taggctaaca agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt
    22141 aagaaaagag ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt
    22201 agtaattcga aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga
    22261 tactcccgta tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca
    22321 tgagaaccat gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga
    22381 tattcttctc ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt
    22441 ggagttttcc tagcctataa ttgggaaagg agtaataatg ataacggtca caaaaaagga
    22501 tttagttgag ctaggatatg gaacttcttt ttcagcagac atcataaaaa aagcaaaaga
    22561 attgatgatt acaaaagggc atatctacta tcaatctcga aagctagata gagtccctcg
    22621 tgaagctgtt gaagaaattc taggaattcg gttttcaaat gaaaacaggt ctgagtagtt
    22681 tttgcacagt aaggagtaaa aactatggtt aaagacccaa taaaaaaagc aaagaatgga
    22741 acctattatt ttcgtgcaaa tctaggttat gattctaaag ggaaaaaaat tcaaaaatat
    22801 cgtagtggat tcactactaa aaaagaagca agagaagaat attcgaaact gctattgatg
    22861 aaaccagaag aattaagtga gaataaggat aaaatgacgt ttaaacatta tatctttgaa
    22921 attttccttc catggtataa gacacaggta aagctcagaa catatgaaaa tcgcttacca
    22981 actattaaga agcatttctc ttattttgat aaaatggctg tttccgatat tgagcctatt
    23041 gatgtgcaaa attggcaact aaaattatca aagaagtgta agtcttctta tgtaagagct
    23101 gttcaaggac tattttcagt tgctatggat cgagcaattg tccttgggat aaccaccaca
    23161 aatccatcta agattattgg caatgtgaaa aagcaaaagt ctaaaattga attttggaca
    23221 aaagaagaat ttgaaaaagt aatctctcta atttacaaag aagattacta tcaacacttt
    23281 ttatttatct ctctgtggtt tttatttatg actggaatgc gaattggtga agctactgcc
    23341 attcaatggg aagatattga ttttgactca ggagtactaa ccatcaacaa aacactctat
    23401 tacaagaatc aatcgaatta tagatttgtt gaaccaaaaa caaaggcgag cgttcgtcat
    23461 attgtattag ataaatgcac attaacttat ttatccgaat ggaaggaagt acaacaaaat
    23521 ctaattcaga cagactttgt aatgagctat aacggtattc ctacccaaaa acatacacta
    23581 tcatatgcta ttgaacgcta tgctaagctg gctggtatcc atcgaatcag aatccatggg
    23641 ttacggcatt ctcatgcttc cttgttaatc agtatgggag aaaatccact tatcattaaa
    23701 gatcgtctag gacacgaaga tattgagacg actttaggaa cgtatggaca tctttatcct
    23761 aacagtaatt tcgaggtagc taacaaatta aatggagcca tttcctttaa agaagcaaaa
    23821 caaaattatg atacttcacc aaagaatcaa tttactgtag actacttgcg aaacaaagaa
    23881 atgaaaaaag tgcaataaaa gtgcagtaaa aagataagaa gagccagaaa cccttggtat
    23941 aagaggattt ctagctctcc tacaactatt cccactccac tgttgctggt ggtttagaag
    24001 tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt
    24061 tttgcaagac ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta
    24121 cagcacggat accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc
    24181 ggatccctgg aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga
    24241 tttcttcacg caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt
    24301 cgccaagaac acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat
    24361 caggcatgcc taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg
    24421 gttcgatcaa ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga
    24481 tcgtctgtgc tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta
    24541 ggaaagagac gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga
    24601 attcattccc aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta
    24661 agaagcgatc tcttgcatcg actttgatga tgttcaagcc gaattttccg cctagacttt
    24721 ccattacttg ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca
    24781 attggtcacc gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg
    24841 aaagaccaag caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat
    24901 cgataaagtt atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc
    24961 gcagcaattc gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa
    25021 aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt
    25081 caaagccttc aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt
    25141 ttggtgtttc tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat
    25201 attcgcggtt tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac
    25261 catagcaaat ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg
    25321 cattctcatc atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg
    25381 cttttacttc ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac
    25441 gaatacgtct tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt
    25501 caacgttcgt catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta
    25561 tctcaaaata gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg
    25621 atatgatttt cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat
    25681 tcctgtaaat ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct
    25741 tcacgatctc cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc
    25801 agtaacgcac caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca
    25861 tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc
    25921 gtattgatcc cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga
    25981 atgatatcat acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt
    26041 acacggttca agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg
    26101 tccatgattc cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc
    26161 agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt
    26221 gtactttttc caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg
    26281 tatttatgaa gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga
    26341 taaatcagat gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca
    26401 ttcaaacttt tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa
    26461 aattctcgcc attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc
    26521 tcctgcgttc tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact
    26581 taagagagat tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt
    26641 cttgacttgc gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga
    26701 tggaagacat gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac
    26761 aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt
    26821 cacgtgaaac agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt
    26881 tacctgatga aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt
    26941 tggcatttgc gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc
    27001 tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata
    27061 tctatgaagc agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg
    27121 ctggaaaaaa agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg
    27181 gtacactgac aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg
    27241 aagaaacatt tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa
    27301 gtgtgaaaga ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc
    27361 atgtacgggt acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt
    27421 ttttcttcaa aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag
    27481 atacagaact tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa
    27541 catactttcc ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt
    27601 agatagaagg gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc
    27661 gtatcagtaa caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag
    27721 acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct
    27781 tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat
    27841 ttcttcttct ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg
    27901 atcatgttat gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt
    27961 tcccaccgct cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat
    28021 gcattagaca gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat
    28081 cgtcgggata acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg
    28141 ctgtttattg aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat
    28201 caattagaag aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt
    28261 aatgggaaat ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa
    28321 aaattactca aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc
    28381 ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata
    28441 ggattcgatg gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag
    28501 ccaagttttg atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct
    28561 caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa
    28621 gtattgacaa agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt
    28681 tagtgctttt tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat
    28741 aatgtataat accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg
    28801 aaaaaaagag ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact
    28861 atacgaatgg aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc
    28921 aagacatgac aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg
    28981 tcatcgattt gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg
    29041 gtgacacgac cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta
    29101 aaatgtctgg acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc
    29161 ccggatttca gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag
    29221 tagcggtaat tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc
    29281 gtgacgtcac agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa
    29341 aaatcgcggc aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca
    29401 tgaagaacat cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg
    29461 ctaaccgaga aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg
    29521 gaaatagtct agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc
    29581 tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga
    29641 caaccgatga agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa
    29701 aattcaaaga aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac
    29761 gaattttgac tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa
    29821 aaatcgtggc aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca
    29881 aagaagaaga tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg
    29941 tgacaaaagg tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca
    30001 ttgaattatt atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc
    30061 atgacatcat cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa
    30121 aagaaatgga actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag
    30181 aagctgtgat gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca
    30241 atccagcatg ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta
    30301 cagtaatcgg gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag
    30361 atgccatcaa taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat
    30421 caaaggatga tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc
    30481 gtgcgcttgt caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag
    30541 cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa
    30601 caggcggagc aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg
    30661 gtgtcaaagc ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg
    30721 gtgctacaag aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag
    30781 gctattaatt ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac
    30841 agctattgaa gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg
    30901 tctcgtaacg gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg
    30961 attaacgaat tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac
    31021 atttacgcat ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg
    31081 tcgacaagta atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa
    31141 tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga
    31201 tctataactc atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga
    31261 ggctttacag aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca
    31321 gggttatttt taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc
    31381 tcatcagaca caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca
    31441 ttcaaccagt cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga
    31501 ggaaatgatg gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt
    31561 gaccaagcat tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca
    31621 gcgatccaag aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc
    31681 atcgatggat tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt
    31741 gcaggtgttg cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta
    31801 aaaggtgaag taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg
    31861 aaagaattag gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca
    31921 gataaaggac gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat
    31981 gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc
    32041 gacaaaaaag tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca
    32101 ttaaacggcg aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta
    32161 gtagaagatt tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt
    32221 tatggattga aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa
    32281 aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact
    32341 cctgaagaaa actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg
    32401 gtttgattag aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg
    32461 gaacaactcc ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt
    32521 agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa
    32581 tattgtcata gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa
    32641 catcaattta caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg
    32701 caaatctaca ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt
    32761 gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg
    32821 gatggtccat cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt
    32881 gggaagcgag cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca
    32941 aaaagtttct gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc
    33001 agttggaatg gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact
    33061 gatttttgat gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat
    33121 catgaaggaa ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga
    33181 aatcaaagca gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt
    33241 caacgttaaa gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc
    33301 attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct
    33361 agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc
    33421 tggcgaagta cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc
    33481 tttgactggt ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac
    33541 taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca
    33601 taaatacggg ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca
    33661 tcaaaaacct tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag
    33721 agaattgatc gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt
    33781 atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct
    33841 gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa
    33901 acgtctgatc gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga
    33961 agaaatttta aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat
    34021 cgttgatccg aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt
    34081 agaagaagca cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa
    34141 aattccgtaa tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga
    34201 tattgatggc tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag
    34261 cccttggttc gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta
    34321 cagcattagg tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac
    34381 aagcactttg cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa
    34441 aagttgtttt gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg
    34501 ctgttccagg tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga
    34561 tgaactatat cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct
    34621 atcgctcaag tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat
    34681 ggttaacaca agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg
    34741 cgcttatttt cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg
    34801 ttggattgaa tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt
    34861 tgtcgatggt catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa
    34921 cgtatcaaaa cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg
    34981 tttccttgct tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta
    35041 ttttgaagat cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca
    35101 atgtatcgat tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca
    35161 tggctaaagt cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac
    35221 caacgaatca aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt
    35281 gatccttact tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac
    35341 tttttctgaa cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc
    35401 ttttagttcc atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg
    35461 gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc
    35521 aacagtcagt ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc
    35581 tgctttaggt gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac
    35641 gcagaatctg gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat
    35701 tttctttaaa aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg
    35761 ggtgatcatc ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca
    35821 agctgccgac acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc
    35881 tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc
    35941 agctggtacg atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg
    36001 gaatccttta ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat
    36061 cattggcgca caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc
    36121 atatttgctg acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc
    36181 aaacgggaag aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac
    36241 gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa
    36301 ggaataatca tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa
    36361 gcaccagatg ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa
    36421 gaagcaggat tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt
    36481 gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct
    36541 gtagggaaag atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca
    36601 tttcgtgtgt ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct
    36661 ggccgtaaag tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt
    36721 gaacaccaaa tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag
    36781 atcgcggcac atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt
    36841 cgtgatataa caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg
    36901 ggtgaaccag gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc
    36961 ggacacacag tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag
    37021 atcaacgata ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg
    37081 gatggcgttg accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc
    37141 aatttagtag attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat
    37201 gcgattgaag aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac
    37261 ttgctaatga ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg
    37321 cgagaatatg taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct
    37381 caaggatatt attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact
    37441 gaaaatggcg aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa
    37501 tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa
    37561 aagaaattga ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg
    37621 aagatgctgt aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg
    37681 ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag
    37741 ggagattcca ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga
    37801 tggccggact aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat
    37861 catttgtacc aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc
    37921 ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt
    37981 atacgcctga ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta
    38041 aagaaggcgt atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa
    38101 tgtctcggac aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag
    38161 cagctcacag cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta
    38221 tgcaagcgaa tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt
    38281 ttaaagcatt ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt
    38341 gtgcatattg aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct
    38401 ttgcgagcta aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta
    38461 cgcggtatgt taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca
    38521 ggaatgggaa tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta
    38581 aagaaattga ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat
    38641 ttagtactag cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc
    38701 tttcattttc ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa
    38761 gaaaagggct atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat
    38821 gatttgacag agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca
    38881 gcggctcttt attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt
    38941 agcgaccatt tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat
    39001 gaaatgatcg aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct
    39061 gccctgcatt aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg
    39121 ctggacttat tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaatct
    39181 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    39241 tattgtgact tgtgaccgag aagttggcag gagaaaaccg tttatacgaa aaaagggact
    39301 gtgacaaatc gtgtcacagt ccctttttta tagtgttttt tataggtagt attttttagc
    39361 aactgttaag tcatcattta attcataaac aagtggttga cctgttggaa tttcaagatc
    39421 catgatgtct tcgtcagaga taccttcgat atgtttagct agggcgcgta atgagttacc
    39481 atgtgcagct actaaaacag ttttgttgtc tagtaatgct ggagcaattt catcttgcca
    39541 gaatggcaaa gcgcgttcta atgtcacttt caagttttct ccaccaggaa tatcgcgttt
    39601 gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct tcgtcagttt cttccattaa
    39661 tggaggaaga gtatcgtaag aacgacgcca gatgtgcact tgttcgtcgc cgtatttttc
    39721 agctgtttct tttttgttca aaccttgtaa tttaccatag tgacgttcgt ttaagcgcca
    39781 tgatttgatt tgtggtaccc aaagttgatc tgaatattct aagatcaagt tacatgtttt
    39841 gattgcacga gttaaaacag aagtgtaagc aacatcaaat tcgattccag cttctttgat
    39901 tttgcgtccg ccttcttttg cttcttcgat tccttctggt gctagattta catcagccca
    39961 accagtaaat tggttcaatg cattccattc actaagaccg tgacgagaaa aaactaattt
    40021 tggcatgttt agctctcctt ttaagatagg tttatatttc tgaaattttc agttctgttt
    40081 tattgtacaa ttttttggaa gagatttcca tcgaaaaatt agataagatt ctatgagaaa
    40141 acaagtagag aatataaaaa aacgaacagt gcccacgcaa ctgttcgttt ttaggtatta
    40201 gatgcataat tgctcgattt ctttttcagg taaattgata tatttataac atgttccaac
    40261 tgaaacaccg cattttgaag aaataaattg gatcgtttct tttttttcat aatagagacg
    40321 tcggattttt ttgatcgttt ttgcatcgat ttttggtctt ccgccgattt ttcctttttt
    40381 acgagcttca tctaatccaa ctaatgtacg ttctttgatc aatgcgcact ccatgtttgc
    40441 taggttttcc attaattgga aatagatttt tcccatttcg cgcgtatcga ttccttcatc
    40501 aagactaatc aagtgcagcc cgcgatcttt gaataattgc gtgagttctg ttaattgccg
    40561 cgtagatttg cctaatcgat gaagttggca aatgactagt gcatcgcctt gttgcatgtc
    40621 agcgatcact gactccaaat gaacagcagt agaatcattt tgttccactt catatgtctc
    40681 atggaaaatt tcttgacaac caaagtcaga taattgatca acctgagttg ttaaatcaac
    40741 gtcatttatg gttgtgcgtg cataaccaat aattttcatg gtgattcctt tctagcagtg
    40801 attttctaac tggatataac agtaaaaatg tagcataaag tacgtcaaaa agatagtttt
    40861 ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa gcagtttttc tttatttacg
    40921 acggattgcg tcaagaatct caacgccttt ttctgagccg acaactactt tagaaaccat
    40981 gtttaggaaa agcccatgtt ctactacccc aacaagttca tctaaatagg tagctaagct
    41041 ttctggctgt tcgatgactt ctagatgcag atcaatgatg taatgaccac catctgtggt
    41101 taagacttct ccgttttcgt ctgtacgcaa gacgggttgg aatctttttt cttcaaaaat
    41161 atgaagtagc tgttgggagc cataagggat cacttcaact gggagtggga acttcccaag
    41221 tttatcgact aatttagaag aatcaacgat ccaaatcgtt tcttttgagt acgtagcgac
    41281 tattttctcg aaaagcaaag cagcgccgcc gcctttgatc ccttgaaaat cagcactgat
    41341 ttcatcagca ccgtctattg tcaaatcgac tacggggact tcatcaatac tctttaacgg
    41401 tattcctaat ttttccgctt gttcttttgt cgcatttgaa gtagtaactc ctgtgatacg
    41461 taaaccttct tcacgcatac ggcgaccaat ctcttctacc atataatagg cggttgaacc
    41521 ggtcccgaga cctacgatca tcccgtcctt tacgaatttc gcggcttcaa tgccagctaa
    41581 ttgttttaaa ttcatctttg cgtccacctt tttttagttt gagcatcgtg ctttcctcac
    41641 tattttagcg tgatttacgg taaaaagata ccattctttt ttattccaat aaagcttttt
    41701 caaaaaaaat tgcatctctt gttgacatgt tttttcattc gtgatattct aacaaaggtg
    41761 ctttatctac aggtttctgt gaagaaacgt gctgactgtt tatcaaagca tatttgataa
    41821 ggatgcagac attgcattta ttttttatca caaaaaaaga accacctgga tgtgtggaac
    41881 tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta caattaacca attagtacgt
    41941 aaacctcgta aatcaaaggt ggaaaaatct aattcacctg ctttgaacaa aggatataac
    42001 agctttaaga aaactcaaac taacgtaaac tcacctcaaa aacgtggggt agcaactcgt
    42061 gtgggtacaa tgacacctaa aaaaccgaac tcagctttac gtaaatatgc acgtgttcgt
    42121 ttgtcaaact taattgaagt tactgcttat atcccaggga ttggtcataa cttacaagaa
    42181 cacagtgttg tattattacg cggtggtcgt gtaaaagact taccaggggt acgttaccat
    42241 atcgttcgcg gtgctttaga tactgctggt gttaacgacc gtaaacaaag ccgttctaaa
    42301 tacggaacta aaaaacctaa agcttaattt taattattaa acaaatgaaa cttgcttcta
    42361 ctatatagaa gtaattacta tgaacaatat ttcggaagga ggagttacgg atgccacgta
    42421 aaggtcctgt tgcaaaacgt gatgttttac cagatcctat ttataactca aaattagtaa
    42481 ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg gattgcagct aacattatct
    42541 ataatgcctt tgacttgatc aaagaatcta caggtaacga tccattagaa gtgtttgaac
    42601 aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc acgtcgtgtt gggggttcaa
    42661 actaccaagt accagttgaa gttcgccctg aacgccgcgt tactctaggt cttcgttggg
    42721 tagtaaacta cgctcgcttg cgcggtgaac acacaatgga agaacgctta gcaaaagaaa
    42781 tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa acgtgaagac actcataaaa
    42841 tggctgatgc caaccgtgcg tttgctcact atcgctggta agattctctc tgtttggtcg
    42901 gcttttataa gtcgacctca gatagtgtcc ttatttttag caactagata gtaaagagag
    42961 gagaaacaag gagaatgacc agagaatttt ctttacaaaa cactcgtaat atcggtatca
    43021 tggcccacat cgatgctggt aaaacgactg ctactgaacg tatcttgtac tatactggta
    43081 aaatccataa aattggtgaa actcatgaag gagcttcaca aatggactgg atggaacaag
    43141 aacaagaacg tggtattacg atcacttctg ctgctactac agcacaatgg aagggtcacc
    43201 gaatcaacat catcgacaca ccaggacacg tagacttcac agttgaagtt gaacgttcat
    43261 tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca atcaggtgtt gaacctcaaa
    43321 ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc tcgtatcgta tttgcaaaca
    43381 aaatggacaa gatcggtgct gacttcttat attcagtatc aactttacat gaccgtttgc
    43441 aagcaaacgc tcacccaatc caattaccaa ttggtgcgga agacgacttc acaggaatta
    43501 tcgaccttgt aacaatgaag gctgaaatgt atacaaacga tcttggaaca gagatcgaag
    43561 aaacagaaat tcctgaagaa taccgcgaat tagctgaaga atggcgcgaa aaattggttg
    43621 aagctgtagc tgaaactgat gaagaactta ctttgaaata cttagaaggt gaagaaatca
    43681 ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt taacgtagaa ttctacccag
    43741 tactatgtgg ttcagcattt aagaacaaag gggttcaatt actattggac gctgttcttg
    43801 attacttgcc atcaccgtta gatatcccag ctatcaaagg gattgaccct aaaactgatg
    43861 aagaagtaga acgtcctgca gacgacagcg ctcctttctc agctttagca tttaaagtta
    43921 tgactgaccc attcgtaggt cgtttaactt tcttccgtgt gtactctggt gttttacaat
    43981 caggttcata cgtacaaaac gcaacaaaag gcaaacgtga acgtgttggt cgtatcctac
    44041 agatgcatgc caactctcgt tcagaaatct ctgaagttta cgctggagat atcgctgctg
    44101 ctgttggttt gaaagacaca actactgggg acacactatg tgatgaaaag agtctagtta
    44161 ttcttgaatc catggaattc ccagaaccag ttatccaagt cgctattgaa cctaaatcaa
    44221 aagctgacca agataaaatg ggtgtggctt tacaaaaact atctgaagaa gatcctactt
    44281 tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat cgctggtatg ggtgaacttc
    44341 acttagatat catcgttgac cgtatgcgtc gtgaattcaa agttgaagct aacgtaggtg
    44401 cgcctcaagt atcttaccgt gaaactttcc gtgctggcac tcaagccgaa ggtaagtttg
    44461 tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg gattgaattt acaccaaatg
    44521 aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg tggggttgtt cctcgtgaat
    44581 acattccagc tgttgaaact ggtttgaaag atgctatgga aaatggtgtc ttagctggct
    44641 atccattagt agatatcaaa gctaaacttt acgatggttc ataccatgat gtcgattcaa
    44701 atgaaactgc cttccgtgtt gctgcttcta tggcattacg tgcagcagct aagaaagcta
    44761 atccagtaat ccttgaacca atcatggctg tagaagttgt tatccctgaa gattatttag
    44821 gtgatgttat gggtcacgta actgctcgtc gtggacgtgt tgaaggtatg gaagctcgcg
    44881 ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc tgaaatgttt ggttatgcta
    44941 caactcttcg ttctgctact caaggtcgtg gtacatttac aatgacattt gaccactacg
    45001 aagatgtacc aaaatcagta caagaagaaa tcattaagaa aaacggcgga aaagctgaat
    45061 aatttcttaa aaccggatag tcctgcaaag agattatcca aagagactat atttttaata
    45121 tagtttcgtg taaaataatt atcgatgata tgggcatgtg gtgtaccttg tgtacatcat
    45181 atacctatca gacaaataaa aaatatattg tatttttagg aggacatttt aaaatggcaa
    45241 aagaaaaatt tgaccgttct aaaccacacg ttaacattgg tactatcgga cacgttgacc
    45301 atggtaaaac tacattaaca gctgcaatca caactgtact atctaagaaa aacggcggcc
    45361 aagctatggc ttacgatcaa atcgatggtg ctccagaaga acgcgaacgc ggaatcacaa
    45421 tctcaacagc tcacgttgag tatgaaacag atacacgcca ctacgctcac gttgactgcc
    45481 caggacacgc ggattacgtt aaaaacatga tcactggtgc tgctcaaatg gacggagcta
    45541 tcttggtagt ttctgctgct gacggcccaa tgcctcaaac tcgtgaacac atcctattgt
    45601 ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa caaagtagac atggttgatg
    45661 acgaagaatt actagaatta gttgaaatgg aagttcgtga cctattaaca gaatacgaat
    45721 tccctggtga cgatgttcct gtagttgctg gatcagcttt gaaagctcta gaaggcgacg
    45781 cttcatacga agaaaaaatt cttgaattaa tggctgcagt tgacgaatac atcccaactc
    45841 cagaacgtga caacgacaaa ccattcatga tgccagttga agacgtgttc tcaattactg
    45901 gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca agttcgcgtt ggtgacgaag
    45961 ttgaagttgt tggtattgct gaagaaactt caaaaacaac agttactggt gttgaaatgt
    46021 tccgtaaatt gttagactac gctgaagctg gagacaacat tggtgcttta ctacgtggtg
    46081 ttgcacgtga agacatccaa cgtggacaag ttttagctaa accaggtaca atcacacctc
    46141 atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga agaaggtgga cgtcatactc
    46201 cattcttcac taactaccgt ccacaattct acttccgtac aactgacgta acaggtgttg
    46261 ttgaattacc agaaggaact gaaatggtta tgcctggcga caacgtaaca atggaagttg
    46321 aattaatcca cccaatcgct atcgaaaacg gtactaaatt ctcaatccgt gaaggcggac
    46381 gtacagttgg tgccggtgtc gttacagaaa tcaaagctta attcacttaa gttttaaagt
    46441 aaagcaacat ttattcggac gtaatcgctc ggaacgaagg tcttaggatc tttgttccga
    46501 gcttttttat ttattgcagg atcaggaaaa aagacaggag tattataaat agaagaaaca
    46561 cgaaataaaa gagtcagtat tttctaaatg aaactttatg aaaacaaaat gttttttaca
    46621 agaaaaagcc ttgctttgtg agtttatata tattataatg acaaaggtgc gtttacttta
    46681 gtgaattgca cagacgtaat cagaatgatt tcgtcggcta agaaacgaga ggttgcgaca
    46741 cgcccggacg ctttgccacg aacgagcgtg acggaaattt tcgtggagct atgtctactt
    46801 ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat ccgtttaaaa
    46861 gcgtatgaac accgtatttt agatcaatca gcggataaaa tcgtggaaac tgcaaaaaga
    46921 actggagcta gcgtgtctgg tccaattcca ttaccaactg aacgcagtct ttacacaatc
    46981 atccgtgcga ctcataaata caaagattca cgcgaacaat tcgaaatgcg tacacacaaa
    47041 cgtcttatcg acattgtgaa cccaacacca aaaactgttg atgctttgat gaagctagac
    47101 ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa taatggaggt
    47161 gtactcatga ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat cttcactgaa
    47221 tctggtgaat taattccagt aactgttgta gaagctactc caaacgtagt actacaagtg
    47281 aaaacaatgg aaacggatgg ctacgaagct attcaagttg gttaccaaga tatgcgtgaa
    47341 gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag caaacacggc tcctaagcgc
    47401 ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa agaaattaca
    47461 gtagatgttt tccaagcagg agacattatt gatgtaacag gtactacgaa aggtaaagga
    47521 ttccaagggg ttatcaaacg ccacggacaa agccgcggac caatggctca cggttctcgt
    47581 taccatcgtc gtcctgggtc aatgggtcca gttgcaccta accgtgtatt taaaaacaaa
    47641 cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc aaaacttgga agttgtacgt
    47701 gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc taaaaaatct
    47761 ttaattacta tcaaatcagc tgtgaaagct aaataattgt aggaagaaag gaggaactaa
    47821 ggaatgccga atgtagcatt attcaaacaa gatggaagcc aaaacggtga aatcacactt
    47881 aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg tttacgatgc aatcgtaatg
    47941 caacgtgcgt cattaagaca aggaacacac gcggttaaaa accgtagcgc tgttcgcggc
    48001 ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg atctatccgc
    48061 tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa caccacgttc ttacagctac
    48121 aaacttccta aaaaagttcg tcgtttagca atgaaatctg tattgtcaga aaaagttgct
    48181 gaaaacaact tggtagcaat cgaaggcttg aacttcgacg caccaaaaac aaaagaattc
    48241 aaacaagttc ttgctaactt atctatcgac tctaaagtat tggttgttct agaaaaaggt
    48301 aacgactttg ctgctttatc agcacgtaac ttaccaaacg tttctgtagt aacttctgat
    48361 aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca aactgctctt
    48421 actcaaattg aggaggtgct tgcataatga acttactaga cgtaatcaaa cgcccagtga
    48481 tcactgaaaa atcaatgctt gccatggatg acaagaaata cacttttgaa gtggacactc
    48541 gcgcaaacaa aactttagta aaacaagcgg tagaagctgc ttttgacgtt aaagtcaaaa
    48601 acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat gcaggatata
    48661 caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa atccaattat
    48721 tcgaagctgc tgaataagca ctaatagata actcattaaa ttaggaggga aaaacgtggc
    48781 gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt ctgattttgc
    48841 tgaaatcaca acatcaacac ctgaaaaatc attgttacaa ccattgaaaa accatgctgg
    48901 acgtaacaac aacggccgca tcactgtacg tcatcaaggt ggcggacaca aacgccaata
    48961 tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt gcaactgttc aaacgatcga
    49021 atatgatcca aaccgttcag ctaacatcgc gttagtacac tatgaagatg gagtgaaagc
    49081 ttacatctta gcacctaaag gactagaagt gggtatgaaa cttgtttcag gtccagaagc
    49141 agatatcaaa gtcggaaatg ctcttccatt agaaaacatt ccagttggta ctgttatcca
    49201 caacattgaa atgaaacctg gtaaaggtgg acaattgatc cgttctgctg gtacaagtgc
    49261 tcaagtactt ggtaaagaag gcaaatacgt attgatccgc ttgaactctg gtgaagttcg
    49321 tatgatctta gctgcatgcc gtgctacaat cggttctgta ggtaacgaac aacacgaatt
    49381 aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa ctgtacgtgg
    49441 tagcgtaatg aaccctaatg atcacccaca cggtggtggt gaaggtaaag ctcctatcgg
    49501 acgtaaagca ccagtttctc cttggggtca accagctctt ggttacaaaa ctcgtaacaa
    49561 aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa aataaataat tactttgacc
    49621 atgtgtcgca cattttgaga ggaggttcac catgggtcgt agtttaaaga aaggaccttt
    49681 tgtcgatgag catttaatga aaaaagttga agctcaagca ggggttgaaa agaaaaaagt
    49741 tatcaaaact tggtcacgcc gttcaacagt tttcccaagt tttgttggat ataccatcgc
    49801 agtttatgat ggacgtaaac atgtaccagt ttacatccaa gaagacatgg taggacacaa
    49861 attaggtgaa tttgcaccta caagaactta tcgtggacat gctgctgacg ataagaaaac
    49921 gaaacgctaa tttgagggga ggataaatcc aaatggcaga acaaatcaca tcagctaaag
    49981 caactgcaaa aacagttcgc acttcacctc gaaaagcccg tttagtaatt gaccttatca
    50041 gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac aaatctgctg
    50101 gaatcattga aaaagtattg atgtcagcag ttgcgaatgc agaaaataac tttgacttag
    50161 atgttgaaaa cttggtagta agcgaagctt ttgttaacga aggacctaca atgaaacgtt
    50221 tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa acgtacaagt catatcacgg
    50281 tagtcgtatc agaaaaataa ggagggataa tcagtgggtc aaaaagtaca tccaattgga
    50341 atgcgtgtag gcatcatccg cgactgggat gctaaatggt atgctgaaaa agagtatgct
    50401 gagttcttac acgaagattt aagaatccgt aaatttattt caactaaact tgctgatgct
    50461 gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg taaacatttc aattcacaca
    50521 gctaaaccag gtatggttat tggtaaaggc ggttctgaag tcgaaagcct aagaaaagaa
    50581 ttaaacaaat taactggtaa aagaatccat atcaatatcg tggaaatcaa aaaaccagat
    50641 ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg tgttgctttc
    50701 cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca agggatcaaa
    50761 actcaagtat ctggtcgtct aaacggagca gatatcgctc gttcagaagg atactcagaa
    50821 ggaactgttc ctcttcatac tttacgtgcg gacattgatt acgcatggga agaagcagat
    50881 acaacatacg gaaaactagg agttaaagtg tggatctatc gtggagaaat tcttccaact
    50941 aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt acctaaacgt gtaaaacacc
    51001 gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa gtagcattcg
    51061 gtgaatacgg tttgcaagct gttgattcac attggatcac aaaccgccaa atcgaagctg
    51121 ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa agtatggatt aaaattttcc
    51181 ctcacaaatc ttatactgcc aaagcaattg gggtacgtat gggttctggt aaaggggcac
    51241 ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc gcaggcgttc
    51301 ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca caaattacca atgaaaacta
    51361 agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga aggttaaaga aatcagagaa
    51421 ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt gtttaatctt
    51481 agattccaat tagcaacagg tcaattagaa aacactgcac gtattaaaga agtacgtaaa
    51541 tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg gaaggaggcc
    51601 attaagcgta tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg cgtggtttca
    51661 gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa agaaaaacca ccctatctat
    51721 ggcaaacgca tgaactattc taagaaatac aaagtgcatg atgaaaacaa cacagcaaaa
    51781 gttggcgaca tcgtgagaat tatggaaact cgtccattat cagctacaaa acgtttccgt
    51841 ttgttggaag tagtcgaaga agcagttatt atctaataac acgagatttt cctatataga
    51901 ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc aagcagaaag tcgtttaaaa
    51961 gtcgctgata actcaggcgc acgtgaaatt ttgacgatca aagtccttgg tggatctggt
    52021 cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc aacgccaggt
    52081 ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa atcaggagct
    52141 cgtcgtacag acggttcata tattaaattt gatgaaaatg ctgcagtgat tattcgtgat
    52201 gataaaagcc ctcgtggaac acgtatcttt gggccagttg cacgtgaact acgtgaaaac
    52261 aacttcatga agatcgtttc actagcacca gaagttttat aatcttgaga cacttatcaa
    52321 aggaggtgcg aaacagttat gtttgttaaa aaaggcgata aagttaaagt tatcactgga
    52381 aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga taaagttatt
    52441 gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc tcctcaaggt
    52501 ggaattgttg agatggaagc gccaattcat gtttctaacg tgatggtggt tgactctact
    52561 ggtgtagctg gccgagttgg ctacaaagaa gtagacggca aaaaagtccg tgtttctaaa
    52621 aaaaccggtg aagttttaga taaataaaag tttaaggaag gagggtctta ctgaatgaac
    52681 cgcctaaaag aaaaatatct taaagaagta actccatcat tgatggaaaa atttaactat
    52741 agctctgtta tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg tgttggtgat
    52801 gcggtatcaa acgctaaaaa cttagataaa gcagttgaag aattaacttt gatcactggt
    52861 caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg gattccgttt acgtgaagga
    52921 atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt cttagacaaa
    52981 ttggtttctg tttcactacc acgtgtacgt gacttccatg gtgtaagtaa aaaagccttt
    53041 gacggtcgtg gaaactatac tttaggtatt aaagaacaat tgatcttccc agaagtagat
    53101 tatgatttag tagataaagt acgtggtatg gacatcgtta ttgtaacaac agcaaactct
    53161 gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa ataattaaag
    53221 gaggcgaact acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc tgcaaaattt
    53281 tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc cacattcagt ttatcgtaag
    53341 ttccatcttt gccgtatttg cttccgcgaa cttgcctata aaggtcaaat tcccggcgtg
    53401 aagaaagcta gctggtaaaa agtaaattcg cataagaagg aggtaaaaga tcaatggtca
    53461 tgacagatcc aattgcagat tttctaactc gtatccgtaa cgcaaacatg gttaaacatg
    53521 agtctttaga agtacctgca tcaaaaatta aacgtgacat cgctgaaatc ttgaaacgtg
    53581 aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg atccgtgttt
    53641 tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt atctctaaac
    53701 caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa agtattgaac ggtttaggta
    53761 tcgcaatcat ttcgacttct gaaggtgttg tcactgataa agaagctcgt gaaaaaaata
    53821 tcggcggcga agttatcgct tacgtatggt aatcaagtag aatctacaag gaggtgtctt
    53881 taagtgagcc gtatcggaaa taaagtcgtt gttatccctg aaggagtaac agttactcaa
    53941 gacggaaaca acgttacagt taaaggacca aaaggtgaat tgactcgtac tttttcagca
    54001 gatatcaaaa tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa cgacagtaaa
    54061 gaaatgaaaa caattcatgg aacaactcgt gcgaacttca acaacatggt tgtaggtgtc
    54121 agcgaaggtt tccaaaaagc gttagaacta atcggggttg ggtaccgtgc acaattacaa
    54181 ggatcaaaac ttgtattgaa cgttggttac tcacatccag tagaaatcac accaccagct
    54241 ggagtaactg ttgaagtacc agctaacaca caagtaattg ttaaaggtgc gaacaaagaa
    54301 gaagttggcg aattagctgc aaacattcgt ggaactcgtc ctccagaacc ttataaaggt
    54361 aaaggtattc gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac tggtaaataa
    54421 tcgtttagaa aaattgcata gaagccagca gcaggaaaaa ggcatttggc tgaagtttac
    54481 cttcaaaaaa atacattttt acttgtgccg cagcttctat ttttcgaaac gtatttactg
    54541 cttgaaatag tagtacaata aaaataaaga ggtgacaatt gtgattacaa aaccagataa
    54601 gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg gtactgctga
    54661 gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa ttattgatga
    54721 cgtagcgggt gtaacgctag caagtgcctc taccttggat aaagaaattt caggtggaac
    54781 aaaaacagaa gcagcacaag ctgtcggtaa actagttgct gaacgtgcag ctgaaaaagg
    54841 cattaaagta gtagtctttg accgtggtgg atacctttac catggccgtg tgcaagcttt
    54901 agctgaagct gctcgcgaaa atggactaga attttagaag aaggaggaat accattcatg
    54961 gtttatatcg atccaaaaca tttggaatta gaagaccgcg ttgttgcaat taaccgtgta
    55021 acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt tgtcggtgac
    55081 aaaaacggac acgtagggtt tggtacaggt aaagctcaag aagttcctga agcaatccgt
    55141 aaagcaattg aagatgcgaa gaaaaactta gttgaagtac ctatggttgg ttcaacaatc
    55201 ccacacgaag taatcggtgc ttttagtggt ggtagaatcc taatgaaacc tgctgtagaa
    55261 ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat tagaattagc tggggtagca
    55321 gatatcactt ctaaatcttt aggctcaaat acaccaatca acgttgttcg cgcaacagtt
    55381 gaaggtttga aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg caaatcagtt
    55441 gaagaattaa tcggataagg aggacaacaa taatggctga attaaaagtt actttaaaac
    55501 gcagtattat cggacgtcct caaaaccaac gcgatacagt taaagcgtta ggtcttacaa
    55561 agattaacag ttcagtggtt aagccttcta atgaagcaat taaaggcatg atcaatactg
    55621 tttctcacct agtggacgtt gaagaaattt aatttagaaa aatcataact agaacattaa
    55681 ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc acgccaagta
    55741 cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg acgcggacaa
    55801 aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag gatttgaagg gggacaaaca
    55861 ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa agactatgca
    55921 gtcattaatc ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac tcctgttgct
    55981 ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt agctaatggg
    56041 gaactaaaca agaaattaac tgtgaaagca gcaaaattct ctaaagcagc tcaagaagca
    56101 attgaagcgg ctggtggatc aatcgaggtg atctaatgtt tagactatta aaagatgctt
    56161 ttaaagtaaa agacattaga tcgaaaatcc tatttactgt atttgtgttg tttgtatttc
    56221 gcctaggtgc acatattact gtaccaggcg taaatgctgc ttcattgcaa aacctttctt
    56281 ctttgccatt tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa aacttttcca
    56341 ttttctcaat gggggtttca ccatacatta ctgcttctat cgtcattcag cttcttcaaa
    56401 tggacatcgt gcctcgattt gttgaatggt cgaaacaagg ggaagttggt cgtaaaaaac
    56461 tgaatcaagc gactcgatat ctgacattag tattggcatt tatccaatct gtcggtatca
    56521 ctgctggatt caatcaatac gctcaattag gtttcgtgaa taatcctaac gtagcaactt
    56581 acgtaacgat cggtttgatt ctaacagccg gtacgatgtt tgttacttgg ctaggagaac
    56641 agatcacaga aaaaggtatc ggaaatggcg tatcaatgat catttttgca ggtatcattt
    56701 ctagattgcc tgaaagtatc agagaaattt atgaagatta ctttataaat attgatcctt
    56761 cagacatatg gaaatctgct atttttgtgg ccattttact agtagcggtc ttgttgatcg
    56821 tgacattcgt aacatttttc caacaagctg aacgcaagat cccgatccaa tatacgaaac
    56881 gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa agtaaatgca gctggagtta
    56941 ttccagttat ctttgctagt tcgtttattg ctacgccaaa tgctgtttta caagcattga
    57001 gcggtagatt cggttctgaa aactggtaca aagtgatgat ggagatcttt aactataata
    57061 cagcacctgg tgctacgatt tatacgattc tgatcgttgc cttcactttc ttctacgcct
    57121 ttgttcaagt aaaccctgag aaattagcgg aaaacttgca aaagcaagga agttatatac
    57181 caagcgtacg ccctggtaag gggactgaag agtatgtctc aagattactg atgcgtttaa
    57241 gtacagttgg tgcagtcttc cttggtttag tggcattgct tccaatcatt gctcaaatgg
    57301 tttgggatct tccacaatca atcggattag gcggtacaag cttactgatc gttatcggtg
    57361 tagctttaga aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa tatgttggat
    57421 tcattgagta agaaagcgtg ttgaggtttc ccctcaacac acgttctcac acatttatta
    57481 ggagggaaaa caatgaacct cattttaatg gggctgccag gcgcaggtaa aggaacacaa
    57541 gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct caacaggaga tatgttccgc
    57601 gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa agtcttatat ggataaaggt
    57661 gcattagttc ctgatgaagt aacaaatgga atcgtgaaag agcgactagc tgaaccagat
    57721 acagagaaag gcttcttatt agatggcttt ccacgtacgc tagatcaagc aaaagctctg
    57781 gacgcaatgc taaaagattt gaataaaaaa attgatgctg tcatcgatat ccatgttggt
    57841 gaagaaatct tggtcgaacg tctagccggc cgatttatct gcagcaattg tggagcaact
    57901 taccataaaa ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg cggtgggcat
    57961 gaattctatc aaagagaaga tgataaacct gagacggtta aaaatcgtct ggctatcaac
    58021 atcaaaaata gtgaaccaat cttggcttat tataaagaac aaggtttgct aaacactatt
    58081 gatggtgctc gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat tgaaaaatag
    58141 cacgattgcc cgtagccgac ttgcattatg cagttactct tgtcaagttc aataaattat
    58201 gatagaatat gtcagtccga cttctagaga atcctctaga tgatccattt tgagaatttt
    58261 gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca agtacaagga
    58321 ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg tcgaaacttt
    58381 gccgaatgca atgtttaaag ttgaactaga aaacggacac caagttcttg ctactgtttc
    58441 aggtaaaatt cgtatgcatt acatccgtat cttacctggt gacaaagtaa cggttgaatt
    58501 gtctccatac gatcttaacc gtggccgtat tacttatcgc tttaaataat tgtactccgt
    58561 aaaactacag gaggtattat catgaaagta agaccatcag taaaaccaat gtgcgaacat
    58621 tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa tccaaaacat
    58681 aaacaacgtc aaggataatt ggaggtgcta taaatatggc tcgtattgca ggagtagata
    58741 ttcctcgtga taaacgtgta gtagtttcac ttacttatat ctatggtatt ggtaacacta
    58801 ctgctaaaaa gatcttagct gatgctggtg tatctgaaga tgttcgcgtt cgtgaattaa
    58861 cgaatgaaca aacagatgct atccgtgcag aagtagataa attaaaagtt gaaggggatc
    58921 ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga aatcggttct taccgtggaa
    58981 tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat gcacgtactc
    59041 gtaaaggccc agctcgcgct atcgctggca agaaaaaata attcttaagt gaaggaggtt
    59101 aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag aatatagaag
    59161 caggtattgc acatatccat tctacattca ataacactat catcatgatc actgatactc
    59221 acggaaatgc attagcgtgg tcatcagctg gttcattagg tttcaaagga agcaaaaaat
    59281 caactccttt tgccgctcaa atggcagcag aaactgctac aaaagcagca atggaacacg
    59341 gattaaagac tgttgaagta acagttaaag gacctggttc tggacgtgaa gcagcaattc
    59401 gttcattaca agctacaggt ttagaagtga ctgcaattcg tgacgtgact ccagttcctc
    59461 ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc attttgagtt
    59521 ttatcagtaa cgtcattgaa caagacactt ttcgttttga aaggggtaaa gataagaatg
    59581 attgagttcg aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta tggcaagttc
    59641 atcgttgaac ctcttgaaag agggtatggg actactttag ggaattccct acgtcgtatt
    59701 ttattatctt ctttgccagg tgctgccatc actaatattc aaatcgatgg tgtactgcat
    59761 gaattctcta ccatcaaagg tgttagagaa gacgtcactc aaatcatctt gaacattaaa
    59821 ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga tattaccgga
    59881 cctgctacag taactgctgg cgatattatc gttgatagtg atgtagaaat tcttaacaaa
    59941 gatatgtaca tctgtagtgt ctctgaagga gctacgttcc atgctcgctt aacagtgaaa
    60001 cctggacgtg gttatgttca agcagatgaa aataaaaaag aagatatgcc aattggcgtt
    60061 cttccagtcg attcaatcta cacacctgtt cgtcgtgtga actatcaagt tgagaacaca
    60121 cgtgttggac accgtgaaga ttttgataaa ttgacgatgg agatttggac agatggttca
    60181 attgaaccgt tggaagcaat gagtttgtct gcgaaaatca tgactgaaca tttggatatt
    60241 tttgtgaact taactgatga agcaaaaaat gctgaaatca tggtcgaaaa agaagaaaca
    60301 caaaaagaaa aaatgttgga aatgacgatc gaagagttag acttgtctgt tcgctcttac
    60361 aattgtttga aacgtgcagg aatcaatact gtacaagaac ttacaaataa atctgagcct
    60421 gaaatgatca aagtccgtaa cttaggacgt aaatcattag aagaagttaa agcaaaattg
    60481 cacgacttag gtttaggttt acgtaaagaa gattaaaacc ttttacgaag gagggaaacc
    60541 aacgtgagtt atcgtaaact aggacgcaca tctagccaac gtaaagcgat gttgcgtgat
    60601 ttaacaactg atttattaat caacgaacgt attgtgacta ctgaagctcg tgcgaaagaa
    60661 gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac gcggagattt gcatgcacgt
    60721 cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga aaatgaggac
    60781 atcgtcattg aatcagcttt acaaaaactg ttcaacgata ttgcacctcg ttacgcagaa
    60841 cgtcaaggcg gatacacacg cattttgaag acagaaccaa gacgcggtga tggtgcacca
    60901 atggttgtct tagaacttgt ctaaaatcac actaataaca tcactttata gatgattctt
    60961 ttagagtgtt atgatgttgg aagatatttt tccgagtcta gctcagcgct gctcctcaag
    61021 cgaatatcgc ttggggagta agcactcatc ataaagtgtt tttttatacc ttcaattacc
    61081 tgtcatgaca gatatgtttg aaggcataaa aaaacatttt aagcgagaaa taattgaaaa
    61141 ttttgtcttt ttttgatagt gttagtagga actttaggat gaagaaggag taatcatgga
    61201 gccaattatt gaattagaaa agataaatta taaataccag ccagatgatc ttcgccccgc
    61261 attgaaagat gtctccttta caattgataa aggtgagtgg atcgctatta ttggacacaa
    61321 tggttcagga aaatcgacac ttgccaaaac gatcaatgga ttgcttctgc cagaatcagg
    61381 gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat atttggacga tccgacagat
    61441 ggttggaatg atttttcaaa atccagacaa tcagtttgtt ggttcgacag tagaagatga
    61501 cgttgctttt ggtcttgaaa atcaagggat tcccagagaa gaaatgctag tgcgtgtgaa
    61561 agatgcactt gagaaagtta gaatggctga gtttgcttca cgcgagcctg ctcgtttatc
    61621 aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt gccttacgac ctgatatcat
    61681 tattttagat gaagcaacca gtatgctaga tccagaaggt cgtgaagaag tgatctctac
    61741 gatcaagaag atcaaagaag aaagtcaatt gaccgttata tctatcactc atgatattga
    61801 tgaagcagct aatgccaatc ggatactagt gatgagacaa ggcgaacttg tccgtgaagg
    61861 tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc gacttaggtc tagatctacc
    61921 atttcctgaa aaactgaaga gcgcactgaa agaacgtggg gttgatgtgc ccagtgaata
    61981 tatgacagaa gaaaggatgg tggattggtt atggacatcc gttttgaaca agtagacttc
    62041 acttatcagc cgaatacacc atttgaacaa agagccttat ttgacatcaa tatgacgatc
    62101 aaagaaaaca gttatactgc attagtaggg cataccggaa gcgggaaatc aaccttactt
    62161 cagcatttga atgcgcttgt taagccaaca agcggtacag tccatattgg agaacgagat
    62221 attcagccgg atacggataa taagaattta aagcccatcc gaaaaaaagt tggcatcgtc
    62281 tttcagtttc cagaagcaca gctattcgaa gaaacggtag caaaagacat tgctttcggt
    62341 cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc tagcaaaaga aacattagaa
    62401 caagttgggc tggatgaaag ctatttggaa cgttcgccat ttgaactttc aggagggcaa
    62461 atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc cggaagtact tgtattggat
    62521 gaaccaacgg caggacttga tccccagggc agaaaagaaa tgatggagat gttttggcgt
    62581 cttcataagg aacagcagat cactattgta ttagttacgc atttaatgga tgatgtagcc
    62641 aattttgcag attatgtcta tgtgttggaa aagggacgaa tcgttaatag cggggagccg
    62701 caagaggtct ttcaaaacat tgaatggcta aaagaaaaac aactaggcgt acctactgcc
    62761 accgagtttg cagaagaatt aatggctaaa gggatgaact ttgctacctt gcctttgaca
    62821 gctgaagaat tagcagacgc tatcgtccat tatgctggag gaaatgccca tgatgaataa
    62881 attgatattt ggtcgttata tgcctggaga ttccttgatc catcgattgg atccccgagc
    62941 taaattatta gccagttttt attttatcgg aatcattttt ttagcaaata attgggaaac
    63001 gtaccttttg ttggcatttt tcacattact ttctgtcttc ttgtcaaaag tagatttgaa
    63061 attttttatc cgaggcattc gcccattgat ctggctaatc ctcttcactg tagctttgca
    63121 ggtcttgttt acaagtggcg gagaaactta ttggagttgg ggtgttttcc atatcactga
    63181 atttggtgtg atgaacggcc tctttatttt ctgtcgattc gtgttgatca tttttatgtc
    63241 gactctactc acgcttacta caccgccttt ggaattatcg gatgcgattg agtacttgct
    63301 tcgtccattg aaagccgtac ggttcccagt ccacgaaatt tcattgatgc tgtcgattgc
    63361 tcttcgtttt gtgcctacat tgatggacga aacagaaaag atcatgaacg ctcagcgcgc
    63421 tcgcggtgtt gattttggtg aaggaaacct agttcagaaa atgaaagcag tcattccttt
    63481 gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa gatcttgcga ctgctatgga
    63541 agcaagaggc tacaaaggtg gggaaggaag aacgaaatat cgtgtattgc attggcataa
    63601 tagagacacg atggttatca ttgcttttgt attattaact gtgctactga tctttttaag
    63661 aggataaacg ttcagtgggc cgctatctat tcttttcttg ctgatctgtt tgcaagaaag
    63721 gatgcaagca gtgtatgaca agaaatatta gggtctgtgg cagactaagg tcacaggcct
    63781 ctttctttta tctgaaaaga aagaacgaaa aaattttata gaaaaatcat tattccagat
    63841 aaccttttag gaacaaagga gagaaaaaca tggtacgtta caaagcaatc attgcgtacg
    63901 acggaacaaa ttttaatgga ttccaaaaac agccaaatgg gcgtactgtt caagaagaag
    63961 tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat cacggtcttt ggttcgggaa
    64021 ggacagatgc cggagtacat gcgatcgggc aagtaatcca ttttgattat ccagaggagc
    64081 gtccgttaga acgaatgcgt ttcgcgctag atacccagtc accagaggat attgctgtaa
    64141 gacaagtaga aattgtttca gaagacttcc acgcaagata tttggtcaaa gaaaagacgt
    64201 atcaatttcg agtggatata gggaaaccac gcagtccttt tcgacgccat tatgcaagtt
    64261 attttcctta cccacttgat ttggaaaaaa ttcagcgggc gcttcctgat ttattaggga
    64321 ctcacgattt cacctctttt tgtgcatcag gaagctcgat agaggataaa atacgtacga
    64381 tctacgaggc taagatggag gtaaatgaaa caaaagacga attgttgttt acatttaggg
    64441 gaaatggctt cttgtataag atgatacgga tcttagtagg aacgcttttg aaaattggca
    64501 atggccgatt gcctgaagat agtatcccag atattattgc gaaaaaagat cgtaatgcag
    64561 caggacctac agcacaccct gaaggacttt atttgtatga agtgaaatat gaataagagg
    64621 ttatgataga agtggtcagc ttcaagaaat aagaaggtat ttacgaaaat tacttctcaa
    64681 aattttgcaa aatttcggct tattttcgaa gaagctactt ctggaacacc gtttaagaaa
    64741 gaggtcgtga gcctgtcatt taactctgag tcacaaagag caatttcaaa aaacagcttc
    64801 tcatctttga tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg
    64861 ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttggtgcg
    64921 gacagtctga acaggttcca tcgtttcttt attttctacg tcttcttctg tgtgtgtttc
    64981 gtggttgttc ttctattgct ggatcaatcg tgccataaaa ggggcgattg cggtcaaggt
    65041 accagacata aagaataaac aatgcaatac ataggacaaa aaggattgca cctatcgtaa
    65101 atccttcagg caagtaactg aaagtgacag tatgtttacc tgctggaact tttatcatca
    65161 agaatgcgtc tttgaaattg ctgaccttga ctggtttccc atcaatttta gctttccatc
    65221 ctttgtcata aggaatagtt gtcaccaaca tggaatcttc agcggtattt acagtcccag
    65281 aagcttttct gccagtggct tccatttcga cacctttttc tttgatctga ttcattgcgg
    65341 cttgataggc tacaacatct agaccaacga ctttaggctc catgaaactt atttctttcg
    65401 tcccataaaa acttgcagta aatgtcactg ttgtctcttg aggataatag ccaagatcat
    65461 agtattgacc agaaatgttg atttgcgttt ttcgcgatgt accattgaca gtgatcgtcg
    65521 cactagaact ttctaattgc gcaaaattgg ttgggaacaa acttaagtaa gcttgtgtat
    65581 ttgcaggcac atggacttcc caagtgagct cttttggctg gttatctgct gtttctgagt
    65641 aagtgacgcc agtcgttgtc tttgtgatcg tcgtattcaa ctgattcttc aaagtaggtg
    65701 tatagaatgt aaaatactgt tcatttagac cagacaatgt attgatcaag tttgtctgac
    65761 tggtcaaatt atctgtcact ggctgctcaa catcattaac ggacaacggt gcagtaaatg
    65821 ctagtggcaa tgcattgaca ttctgataca gtgtatagtc gccgctttga ccagcttggc
    65881 ggaagccata tttactgaac gtgctttcgt ttttagaaat gttatatttg attcctgtaa
    65941 acccatccat cagcaaagta ttattgttat aacggatatt caaatttgtc cctcttgaac
    66001 ggaaacctaa tttatccagg taagaggaag aattacgatt tcttatagaa gaaaacaaac
    66061 tgataccact gtaaccatag ttgatcgaat cgtttgcaga tactggatcc agattttcca
    66121 agcggtaaaa cgagtcggaa tctttttttg tttcatttac aagagctttc aagtcaggat
    66181 aaggatcagt atataaactg cgggaagcat aattccaatc atcaagaatg ccatggatca
    66241 tactgttcgt attgaccagt gcttcagcag acatgatcaa taataacaat atactcagat
    66301 gattgaatga aagtttttta tgctgataaa atcccacagc aaaagcataa atccctaaga
    66361 aaaccactgt caatacaaag gaagtcatcg tcacataggt gtagcttcct tcaggtttga
    66421 tgccccatgc caccgcaaag agagcagcta cgaccaaaat gattcctaac aacagcccac
    66481 catcgtcttg ttctatcttt tcccatcctt ttcctgcaag caggagcact aagaaagaga
    66541 acagataggc ataacggaat aaaaacatat tcggtgcatg cataccatgc cagaacaaat
    66601 tcaatggaac aagataaaaa cttgcgataa gtatggcgaa aagactgccg tacaaaaatt
    66661 tatttttcaa cggtactttc cgactggcaa aataaaacag acagaggact aatggtagca
    66721 gacctgcgta aacgaacgga atcgatccgt attttgtcgt gtcataaaca ccaatcatat
    66781 ttttcatgat tagatccaga taagaagtcg cttctgtttt caacgtagtg atctgactca
    66841 gtgcctcgcc atttgaccga agatccagca ctgctggcaa tacaatgatc attgaagcgc
    66901 cgcctgccag taaagaagtg ataccataag gaacaatcgt ttttttatat tctgaccagt
    66961 ttgtcaatag acgggcgata aaatataaga aagaaaatac gccaatcata aaacccatat
    67021 aaaaactgga taaaaacaag agtaaataac tgataaataa aagtttcggc cgtttaagat
    67081 ccataacacg atgaatcccc aaaatcacta atggaagata aacaaatgca tccagccaca
    67141 tgatgatctc cgaatgtgca gtgataaatg agatcaatgc gtaactgacg ctaagtgcaa
    67201 catgccccca gcgaggaatc ttgaacgtac gtgcaagaaa ccaaaaagcc aatccggcac
    67261 agccgacctt aagcaaagtc agaacataca gtgcatctgg catcatctgg tttggaaaaa
    67321 agaacaccag tggcgtgaag atcccgccta gatagtaaga aattaaagat aaataattca
    67381 atcctagaga agcattccat gtataaaaaa tactttgctt tccaagaaga acatttcgga
    67441 agctggcatg aaaattagaa aactgtgaaa aggcatcact tgccaaaata cttctgctgc
    67501 ttccaggata gattccgata cttagataaa caagtgctaa aatcaaaaac ggaatcagga
    67561 aactagctgc tgcgtagcgt ccgttttctc taaagaatga ctttaaattt tttttcatat
    67621 ttattttcca ccaaatttct atgaagttgt attcagttta tcatatcctt gtttttctct
    67681 gcttaaaaag agtctagttt tcacaaaaaa ataaaattta gaataaataa cttcataatt
    67741 tgttcgtata acaaaaataa acgcaaaaaa agggtaggaa tcaaaagtcc cacccttaaa
    67801 aatttttatt aaataaaaaa ccgttcacta tctgaacggt ccaagtatca ttcaattaaa
    67861 cgcgttcaac ttttcctgat ttcaaagcac gagttgacac ccaaactttt ttaggttttc
    67921 cgtcgaccat aacgcgaact ttttgtaagt taggtttaac agtacgtttt gttgagttca
    67981 tagcgtgtga acggttgttt ccactttttg tcttgcgacc tgtaaagtaa caaacttttg
    68041 ccatggtaat ttcctccttt gccttgattt ggagcaatag aataattcag ctcataccag
    68101 actaacatat catatagaat gggaatttgc aagtacattt tcatagatac aattgacaat
    68161 tgagaagaat aattgtaaat taaacaaggt tatacctgaa attttgcaag gtttttgagt
    68221 actttgggta actgcctttt atttcaagtt gcgctatgat aaaatatgat agaagaaaaa
    68281 tgggagtttt ttacccgtta aggaggctca ttcatggctg taaaaattca aacgtcagct
    68341 ggaaccattg aaattaccaa tgatgtaatt gcaacagttg ttggtggcgc cgcgacagat
    68401 gtgttcggtg ttgtaggaat ggccagcaaa aatcaaatta aagataatat tactgagatt
    68461 ttgcgtaaag aaaattactc aagaggagta gttgtccgcc aagaagataa tggtattgct
    68521 gtagatgttt atacgattat cagctatggt acgaaaatct ctgaagtttc ccgcaacgtc
    68581 caagaaaaag tgaaatacaa tctagaggca ttattaggcg taactgccaa ttcagttaat
    68641 gtctttgtac aaggtgttcg tgtactgcca gactaggcag gagactggct ggtaactcag
    68701 cagtcgatct gaaggaggat tatctaggtg agtgtaacag aaataagtgc aagccagttc
    68761 caggaaatgg tccaggcagg tgcgaatcgt ctgcaagtaa atgcagaata cgtcaattca
    68821 ttaaatgtat tccctgtgcc agatggcgat acgggaacaa atatgaatct atctatgact
    68881 agtggagcta aggcagtagt agattcaacc tcagaaaaag taggagaatt agcagcaatg
    68941 ctttcaaaag gattgctgat gggtgcgcgc ggaaattccg gcgttatttt gtctcaattg
    69001 tttcgtggat tttctaagca aattccagat gttgtctcat taaatgcttc tgatcttgct
    69061 gcagccttca ctcatggagt tgaaactgcg tataaagcag taatgaaacc agtagaagga
    69121 acgattttga cagtagctag agaagcagcg aaagctggag aaaagaaagc aaaaagtact
    69181 gatgatgtcg ttgaagtaat gacagctgtt gtaaaaggag caaagcgtgc tttagctaaa
    69241 actccagact tgcttccagt cctaaaagaa gtcggtgtcg tagacagtgg agggcaagga
    69301 ctgctgtttg tttatgaggg ctttttaagt gcattgaatg gagaattcca agcggatgaa
    69361 acatatgaac cttctccagc agaaatggat gatatggtca atgcagaaca tcatcgcagt
    69421 gtccaaggtc aaatggcgac agaagatatc cattttggtt actgtacaga aatcatggta
    69481 aaaatcggag aaggacctac agtcgatagt acattcgatt acgaagaatt ccgtaactat
    69541 ttagatggta taggagattc tttacttgtg gtcaatgatg acgagatcat caaagtccac
    69601 gtgcatacag aaaacccagg agaagtcttg aattatggac aaaaattcgg ttcattagtc
    69661 aaaatcaaag tcgacaacat gcgcttgcag catgagacga ttttggaaca cgatgaagaa
    69721 gtgaacgagt ttgcgaaaaa agaaatacca cgtatcccgt atggtatcgt agcgatcgct
    69781 gctggtaaag gcgtccaaga gctgtttgaa agtttaggcg ctaattatgt tatcagtggt
    69841 ggacaaacaa tgaatccaag tacggaagac attgttaaag cgattcaaga agtaaacgca
    69901 gataaagtga ttgtcttgcc aaacaataag aacatcttca tggcagctga tcaagcagca
    69961 gaagtagcag atatcccagt agcagttgtt ccttcaagaa cgatttccca aggattgaca
    70021 gctatgcttt cattcaatga tcaagcttct ttagaagaaa acaaagaaac gatgacagaa
    70081 atgttgtcta gcgttgtgag cggtcaagtc actcacgcga tcagagatac ctcaattgac
    70141 ggtgtagaga tcaaagaagg agactttttg ggcatgatcg atggaaaaat cctgatctct
    70201 aacccagata tcacagcaac agcgttaagt acactagaac aaatgatcaa tgaggacaca
    70261 gagatcgtaa cgatcttggc aggtgatgaa ggaacagttg aacaagcaaa tgaatttgct
    70321 gagaaactaa cagaaaaaca tcctgatttg gaaatcgaga tccaccaagg agatcagcct
    70381 gtctatcctt atctattttc tgctgaataa tttctgtgtc agaatatctt tctaacacag
    70441 tattcattca taaaaggcct tcggttagtg atcaggtatg atcgctgacc aagtcctttt
    70501 tttgttaaag gagggaggaa aaatttatgc gttctttaga tgatccaatc actatgctgg
    70561 caggagtcgg agaaaaaaga gcagctagtc ttgccagctt gggcatccaa acgattgaag
    70621 acttactgac ttattatcct tttcgctacg aagatattca ggaaagaaat ctaaacgaga
    70681 tccaggatca agaaaaggtt actttgaaag gaatcgtagt ctcgcctcct gtgatgaatc
    70741 gatttggtta taaaaaaagc cgattgcaat ttcgtatgat gcaagaacat gatgtgttca
    70801 acgtttcttt ttttaatcaa ccttatctaa aagaaaaagt cattttatcg gaggaaatcg
    70861 ccgtatacgg taaatgggat gcaaagcgta aagccttgaa cgggatgaag attttaggtt
    70921 ctcaaacaat ggatgacttt tcgccgatat atcacgtcaa caaatcaatt cgtcaaacga
    70981 cattagttga tctaatcaaa aaagggtttc aagaatacgg cacattgatt gaagaaaatc
    71041 tgccaaacaa ccttgtggag aaatatcgac tgcttgaccg agcttctgct gtaagaagta
    71101 tgcattttcc aaaagaccac gaagaaaacc atcaagccaa acgacgtgtc gtttttgaag
    71161 agttcttttt atttcaaatg cgtcttcaag gattgaaaaa agcagaaaaa gcagaaacga
    71221 atggtttgga gatcctttat gatgtccaaa aattgaaaca atttactcaa aagctacctt
    71281 ttgaactgac aaatgcacaa aaacgtgtaa cgaatgaaat ctgtcgggat cttcgcagtc
    71341 cgcagcatat gcagcgactt ttgcagggag atgtaggtag cggaaaaaca gtggttgccg
    71401 cgattgcgtt atacgctacc gttacagcag gtttccaagg tgcgttgatg gtgcctacag
    71461 aaatcttggc acaacaacat atggaaagct tgcaacagct gtttgatccc aatgaagtaa
    71521 ccatcgcatt gattacgggg tcaacgaaag caaaagaacg acgtgagctc cttgaaaaac
    71581 tagagcaagg ggaaattaac gtcgtgatcg gcacacatgc attgatccaa gatggagtcg
    71641 aatttcagaa tttaggtcta gttattacgg atgagcagca ccgttttggc gtgaaccagc
    71701 ggaaagtact tcgtgaaaaa ggctggcgac cagatgtctt atttatgaca gcgactccga
    71761 ttcctagaac attagctatt acagcatatg gtgagatgga tgtatctatc attgatgaac
    71821 ttccagctgg acgtatcccg atcgaaacac gctgggtaag aacacctcaa ttggacagtg
    71881 tactggattg gacgaaaaaa gaactggcaa gaggacatca aatgtatgtc atctgtccgc
    71941 tgattgaaga atcagaagca ttagatgtaa aaaatgcagt ggaaatctat gaaaaacttc
    72001 gtgaactatt tgcaccaaaa tatgaagttg gcttgttaca tggtaaaatg aagaatcagg
    72061 aaaaagaagt aatcatggaa gccttcaaag acaaccagat gcagatatta gtttcgacta
    72121 ctgtaattga agtaggggta aacgtgccga acgcgaccgt catgctgatc atggatgcgg
    72181 atcgatttgg attggcacag ctgcaccaac ttcgtggacg tgttggacgc ggaagtgatg
    72241 cttcctactg tatcttagtt gctaatccta aaaatgaact aggtgtggaa cggatgaaaa
    72301 tcatgacaga gaccaccaat ggttttgtac taagcgaaaa agatctggaa ttacgtggtc
    72361 ctggtgaagt atttggcttc cgtcaatctg gtctgcctca atttgcgatt gctgacttgg
    72421 tgacggatgg aaatgttttg gaggtagcca gagaagaggc tgcagccctt tggcaaataa
    72481 aggattggca gatgcttcct gagtatcagc ctttagcgca aagcctccaa atagaaaatc
    72541 aggataatcg ttatttcgat tagcagactg tgctatactt tatactgatg acggaaaaag
    72601 gagtggaaac aatgaaaatt gccgtagatg caatgggcgg agatcacgca cctcaagcaa
    72661 ttgtagaagg cgtggcttta gcgcagcaag attttccaga agtagaattc ttactttacg
    72721 gaaaagaagc agaaatcaag aaatacttaa caaatgaaaa aaacatcacg attatccata
    72781 cagacgaaaa aatcaatagt gatgatgagc cagtaaaagc aattcgacga aaaaagactg
    72841 catcaatggt ccttgctgca caagcagtga aaaacggtga agcagatgct gtattttcag
    72901 cagggaatac aggcgcgcta ctagcagcag ggttgttcat cgtaggacgt atcaaaaata
    72961 tcgaacgccc tggcttgatg tctaccttgc cagtagtagg gaaagaaggt gccggtttcg
    73021 atatgctgga tcttggcgct aacgcagaaa acaaaccaga acatttgttg caatacggta
    73081 ttttaggttc tttttatgca cgtaaagtcc gaggtatcgg acgtcctcgt gtcgcactct
    73141 taaacaacgg aacagaagca acaaaaggta gtgaagtgac gaaaaaagcc tatgaactcc
    73201 ttcagaatga aacaagcttg aattttattg gaaacgtaga agctcgtgag ctactcaatg
    73261 gtgtggctga tgtagtggtc acagatggat ttacaggcaa cgctgttttg aaatctattg
    73321 aaggaacagc aatgaatatg atgaatctat taaaatctgc tattctaaat gaagggatca
    73381 aaggaaaaat gggcgctatg cttttaaaag acggcttacg ttctttgaaa gcagaaatgg
    73441 attattccaa acatggcggt gccgttttgt ttggcttgaa agcaccagtc attaaaactc
    73501 atggagcaac aggccccgat gctgttcgct atacgatccg ccaaatccac acaatgcttg
    73561 agacagatgt agttggacaa ttagtcgaac aatttgaaaa aagcgaagaa tagtatttgg
    73621 atatctgaag cgatccgttc cattgaaaaa aaggtggaca ttcctgaaaa atgccagtaa
    73681 aattagccat ggtgtaggta attgacaagt gtatgattct tggaggtgaa aaagttgact
    73741 cgcgaagaag tatttaacaa agtagcgaaa attatttcaa atcattttga gatagatacc
    73801 gataaagtga cggatgaatt gaacatcaaa gatgatttaa aagctgattc aattagtatt
    73861 atggaattcg ttttagagct tgaagatgaa ttcggtacgg aaatttcaga tgaagacgct
    73921 gaacaaattg aaacagtcgg tggcgcagta gattatatca gcagccatct taaataattt
    73981 caaataaaag caaagaagga taaaaagctg catgcagctt tttatccttc tttttttaat
    74041 ctttttgaca cgtaaaagtc atttttctga taattctggc gataaacaga aattatgttg
    74101 caaagtaaag cggttttcta tataattatt aataattcat tacacaaaga taagaataac
    74161 ctttgttata aaacgattta ttctgtgtaa tgtccttttt tattaagaaa aacttccatt
    74221 taggaagcag agaggggtat tcgattgtcc gataaccaat tattagaagt tcagaaccta
    74281 catacaggat tccgactcaa agatgactat tacgatgcag tagatgacgt ttcatttact
    74341 ttagacaaaa acgagatttt agcaatcgtt ggggaatctg ggtgcgggaa aagtaccttg
    74401 gctactacga ttatgggatt acttgatcct aataacacaa aaattacagg tgagatttta
    74461 tataacgatc tgaatttaat cgacctgaac gaaacgctct ataataaaat ccgcggaaat
    74521 gatatcggaa tgattttcca agatccgttg tctgcattaa atcctctcat gagaatcgaa
    74581 gaccagatca aagaaggact ttcctatcac acaaagatga atgcagaaca gcgtcaagct
    74641 cgtgcactag aattgctaga acaagtaggg attcctaatc ctgcgagagt tggacgccaa
    74701 tatccccatg aactatcagg cggtatgaga caacgtgtca tcatcgcaat cgcaatcgcc
    74761 tgcaaacctc caatcgtcat tgcagatgaa ccgacgacag ccctagatgt aaccatccaa
    74821 gcacagattc ttgatttact gaaagacctt caggaagaaa caaaaacagg gatcatcttg
    74881 atcactcatg acttaggagt cgtagcagaa atggctgatc gagtggcagt gatgtatggc
    74941 ggacagttcg ttgaagtagc aggtgtaaaa gaactgtttg atcatcctaa gcatccttat
    75001 acacgctctt tactgaactc tataccacaa gaaggaaatc atgatgcaga actccatgtg
    75061 atcgaaggtg tcgttccttc tctgaaaaac atgcctcgta aaggttgtcg gtttgcacca
    75121 cgtattccat ggataccagc aagcgatcat gaagaagagc ctatccttca tgaagtcgca
    75181 ccgaaccact tggtacgttg ctcctgctat aagcatttcc actttagaga cgaaaaagga
    75241 gaggtgtaga tgacagaatt aatcacaatc aaagacctga aagttcatta ccctattcgc
    75301 agtggattct tcaatcgagt aacggatcat gtatatgctg tagatggtgt ggattttatt
    75361 atcgaaaaag gaaagacata tggtttagtg ggagagtctg gctctggaaa atctactacg
    75421 ggaaaagccg ttgtgggatt agaaaaggta acagccggac aaatcatgta tgaaggaaaa
    75481 gatgtaacga agcgaagcaa ccgcaaaaaa atgggttata acaaggatgt gcagatgatc
    75541 ttccaagatt caatgtccag tttgaatcct aaaaagcgag tgttggatat cattgctgaa
    75601 cccatccgaa attttgaacg gttaagcgat caagaagaaa agaaaaaggt aaaaggactg
    75661 ctagatatcg ttgggatgcc ggaagatgcg ctatataaat atcctcatga attttcaggc
    75721 ggacaacgtc aacgtctagg agtagcccgt gctgttgcta ccaatccaaa actgatcgta
    75781 gccgatgaac ctgtatctgc tctggatcta tctgttcaag cgcaagtatt gaatttcatg
    75841 aaacgaatcc agcaagaata cggcctcagc tatttattca tttcacatga tttaggtgta
    75901 gtaaaacaca tgtgcgacaa tatcgcaatt atgtacaaag gtcgatttgt agaaatcggt
    75961 acaagagaag acatttataa tgacccgcga catatctata cgaaacgtct gctttcagct
    76021 attccgcaaa tcgatgtaga aaatagggaa agccacaaag aaaatcgacg aagagtagaa
    76081 caagagtata tccaaaacca aaaagaatac tatgatgcga ctggtcgagt ctacgatttg
    76141 cgtacgatca caactacaca caaagtggcg ttgaaagatg gaggtgcaag ctaatgtgga
    76201 aaacgattct gcgacgtgtt ttattgatga tcccgcaagt tattatttta agtgttctga
    76261 tcttcttatt agcccaagcg atgccggggg atccatttac cggtttgatc aatccaaatc
    76321 aggatccggc agtgatcgaa cagatgcgac aagcagctgg cttgaatgac ccatggtatg
    76381 tccagtactt ccgttggatc ggaaatgctt tgcaaggcga ttttggtcaa agtttcttat
    76441 acaaacgctc tgttgctgac ttgatcggcg aacggatcat caacacttta tatctatcga
    76501 tcttgacagt tgtccttact tatttgattg ctgtgcctct ggggatgctt gctggccggt
    76561 atcaaaattc aatcttggat aagattgtcg taatctacaa cttctttagt tttgctgttc
    76621 ctttatttat cttcggcttg atcatgctat ttgtttttgg ttatcgttta ggctggttcc
    76681 caacaagtgg atcacaaaca ctaggtttgc aaggtggatt tatcactcaa tggatagacc
    76741 gtttgaaata tattctgtta ccatcaatta cccaagcatt tttggcgact gccgtcacaa
    76801 tccaatatct gcgaagtgaa gtaatcgatt cgaaatcgct cgattttgtc agaacagccc
    76861 gttccaaagg ggtaccgacg aataaagtat tcagtcgaca tattttccga aatgcagcgt
    76921 tgccgatggc ttctcaaatg gggtatgaaa tcacttcgtt gattgctggt tctgtagtta
    76981 tcgaaaagat ttttggttat ccgggagtcg gaaaactatt tattgattcc atcggccaac
    77041 gggactatac cgtgatcaca tccttagtat tgattttagg aatcgcgacg ctagtgggga
    77101 cattactttc agatatcatc atgagtatcg ttgacccgcg tattcggatt caataaaacc
    77161 aatcagaaag gagaatggat cgatgagtga aaaaaacaat acagcagttc aagaaagtat
    77221 tcctcctatg gggatacgaa tgattgctcg agaatttaaa aaagataaaa ttgctatttt
    77281 ttcattaggt ttgctagtgg ttttattatt agtgatcttt atcggtgcga tgctgaccga
    77341 tcaagacaaa gtgatgtatg ttagtatctt cgacaaatac gctgcaccag gaacagtttc
    77401 gacacaagga caaaagttca ttctgggagc agatgaaggc ggacgtgatg tattaggaca
    77461 attgatcata ggtgctagaa actctgttac gatcggattt gcgatcacga tccttacttc
    77521 gatcatcggg gttggattag ggatcatctc tggctactac ggagggatca ttgacaacat
    77581 cttgatgcgg atcgttgatt ttatcatgat tctaccgatc atgctgatca ttatcgtctt
    77641 cgtatcgatt atttccagat acaatgtctg gtcctttata ttcatcatga gtgcatttta
    77701 ttgggtagct aaagcccgat tattccgaag caagacatta tctgaagcaa gaagagatta
    77761 tgtcagtgcc tcaaaaacaa tgggaaccaa tgattttaaa atcatgttcc gcgaaatcat
    77821 gccgaatcta agttcattaa tcattactaa tttgacgatg aactttgcgg caaacatcgg
    77881 tatcgagaca actttgactt tcttaggttt cggcttgccg gcaaatgtac ctagtttagg
    77941 tacattgatc ggttatgcaa gtaacggaga agtcttagtg aatcggcaat ggatctggtt
    78001 acccgcatca atccttattt tagttttgat gttgagtatc aattatgttg gacaagcatt
    78061 caagcgttct gcagatgcgc gccaacggtt aggctagtat agaagcgaag ggggaaatag
    78121 gaatgaagaa aacgattttt ggatttgcag cgctgatgtc tgctgtcgtt cttgcagcat
    78181 gcgggggcgg aggtacagat acctctggaa acaactctgg aggcaacggc aataacaaag
    78241 aggaattaac ttttccaatt gcaacaaaaa ataatgatga tgcaatcgaa gacggcgaat
    78301 tagaagctgc ggtcgcaacg gacagtcagt ttaaaggatt gttccagtgg gagttttacc
    78361 aagattcata tgatgcagct tttatggctc cttctcatga accgctattt gcaaacgatg
    78421 acgatttcag aatcacagac gacggagctg caacgcttga tctagatgaa gatacgaaaa
    78481 aagcgacaat caaagtaaaa gacaacgtca aatggtcaga tggtgaagat gtaacggcgg
    78541 acgatgtgat tttcccttac gaaatcattg ggaataaaga ttacactggt atccgttatg
    78601 acgacacttt ccgtaacatc gtaggtatgg aagaatatca tgatggaagt gccgacacaa
    78661 tctccggaat caaaaaaata gacgacaaaa cagttgaaat cgaatataaa gaagtaaacc
    78721 caagcatgct tcaatcgggt ggtggtatct ggtcatatgc agcaccaaaa catacgttag
    78781 aaggtgtagc aatcaaagat atggaatcaa gcgatcaagt acgtaaaaat ccagtgactt
    78841 ttggccctta ttacatgagt aatatcgttg caggagaatc agtagaatac ttgccaaacg
    78901 agcactactg gaatggtacg cctaaattga aaaagatcac gatgaaatct gtaccgatgg
    78961 caagtgcaac tgaagcattg aactctaaat tatatgatat gattttccaa atgccaacag
    79021 atacttacga tacctataaa gaaattgctg gctatacaaa cttaggccgt gaacaaactt
    79081 cttatactta tctaggattc aagttaggta aatggaatgc agatacaaag agtgtggaat
    79141 acaatccaga ttcaaaaatg gctgataaat cattacgtca agcaatgggc tatgcattag
    79201 ataataatgc agtaggtgaa cgtttctatg caggtttgag aagcaatgcg acttcattga
    79261 ttcctccagt attccagtca taccatgatg atgagatgaa aggattcaca caagatctag
    79321 acaaagcgaa aaaactatta gatgatgctg gctacaaaga tgtggatgac gatggtttaa
    79381 gagaagacaa agatggaaat aaactaacaa tcaactttgc ttcgatgtcc ggtggggaaa
    79441 cagcgcaacc attggcagat tactatatcc aacaatggaa agaaattggt ttggatgtcc
    79501 aatacacaac aggtcgtctg atcgaattca attctttcta tgataaatta gaaaacgatg
    79561 atcctgaaat tgatgtttac caagctgctt ggaatacagg aactgatcca aatccaagtg
    79621 gactatgggg accaaatgct gcgttcaact acactcgtta tgaatcagaa gaaaatacaa
    79681 aattgatcaa cgatatcagt tcaagcaaag catttgatga agagtaccaa aaagaagcat
    79741 atcaaaaatg gcaacaatat gcatttgaag aagcatttgc gattccaacg ttgttccgta
    79801 atgaagtatt gccagtcaac gaccgtgtca aagactggga ttggtcttat gatgtaccaa
    79861 acccatgggc aaatgtttct gttacagcag attcacgttc ataatcacta aaaatgaccg
    79921 aaaaagggcg atgtcctttt tcggtttttt gatatttttc tgtttatttc tctaatattc
    79981 aggtcaaggc gctgatttca tgacttctcc aataaacggt gttcaaagtc cagtttctcg
    80041 gtattaagtc aaatttatgc aaaaatatgg aaagccattt tcacaaaatt ttttcttcat
    80101 actcgaaact aaacgaattt ttcacaactt ctcgtcaatc actaggatta ttaaagcttt
    80161 acttaccttt tgaccgaacc atttcaaatt tgacaaaaaa ccagtataat aaggaagtat
    80221 cgaaaaaata tccataaaag gaatgtggaa aatggacaac cagctgacaa aagaactaaa
    80281 agaaaactat aacatcgttt ttcacgatct aaatttgctt gaacaagctt ttacccattc
    80341 atcctatgtg aatgagcatc gcaatctgca attatcggat aatgagcgac ttgaattttt
    80401 aggagatgcc gttttagaac tgatggtttc tcagtattta ttccgtttat atcctcattt
    80461 gccagaagga aaattgacaa aaacacgagc tgcgatcgtt cgcgaagaca gcttgtcgaa
    80521 atttgccaaa gaatgtcact ttgatcaata tgttttgttg ggcaagggag aagagaattc
    80581 aggaggacgt acgcgaccag cattgctttg cgacctattc gaagcttttt taggcgcgtt
    80641 atatcttgac cagggattcg acacgaccca tgcattcatc gaaaaagtga tttttcctaa
    80701 agtcaaagca ggtgcttttt cacatgagat ggatcataaa acaaaactac aagaagtctt
    80761 gcaaaaatca ggtgatgtat caatcgaata ccgattgatc aatgaagaag gtccagctca
    80821 tgaacgagta ttttggatcg aagtttatgt agatgatcaa ttgattggca ctggacaagg
    80881 gaaatcgaaa aaattagcag aacaagctgc ggcggaaaat gcattggcag cactgtaaaa
    80941 gtaagtgagg gaaagctttg tatttgaaac gaattgaaat agccggtttt aaatcatttg
    81001 cagacaagac agtcattgat tttgaaaata gcgtcacagc agtggttgga ccaaatggca
    81061 gcggaaaaag caatattaca gaggctatcc gctgggtatt aggagaacaa tctgctaaaa
    81121 gtctgcgagg cggaaaaatg ccggatatca tctttgccgg ttccgatacc cgtaagcagc
    81181 tgaatattgc agaagtcaca gtgattttag acaatagtga tcattatctg ccattggagt
    81241 acaatgagat cagtgtaacc cgccgttatc gacggactgg ggaaagcgag tttttcatca
    81301 ataaacaaag ctgcagatta aaagatatcc aagaattgtt cttagattct ggattaggaa
    81361 aagaatcgtt ctcaatcatt tctcaaggaa aagtagaagc gattttcagt agtaaaccgg
    81421 aagaccgtcg tgggatcttt gaagaagcag ctggtgtctt gaaatacaag cagcggaaaa
    81481 agaaagcaga acagaagctt tttgaaacag aagacaacct cagccgcgtc caagacatca
    81541 tccacgaatt ggaagaacag ttaactcctt tggccgccca gagtgaggca gcaaaggagt
    81601 ttttacggct aaaagaaaca ctgactcaaa ccgatgtctc tttgatggtt gctgaaatca
    81661 agacagcaaa aaaagattgg gacaacaaac aagcacagct tgcgaaattc aatctagaat
    81721 taggcaaact atctgagtcg atccaagaac aagaatccat tttggcgaag cagcgtaaag
    81781 aaaatgcgca agccgatcgt ctgatcgaaa aaaatcagca agtattatta gatctttcgg
    81841 aaaaactgaa gcagacagaa ggacaaaaag atgttttgca ggagcggaca aagcatacac
    81901 aaaaaagctc gcaagaatat caaacttcat tagcagaagc acaaaaaaag gttaaacatt
    81961 ttgaaaagct tcaagaaagc ttgatgaaag cagcagcaga aaaagaaaca gagatccaaa
    82021 aagctgaagc aaatctcata aaaacacagc aagaattaga aaaataccaa aaatcaacga
    82081 aagaattatt ggcagaatta agagatcaat atgttgattt gatgcaagaa caagcagctg
    82141 taggaaacga actgaagtat ctagaacgtc aatatataca agaaacagca aaaagcaagc
    82201 agacgttagc caagcaatcg gaagtagaag catcagtaga ccgattgatc ttacaaaaag
    82261 aagaattgac acaaaagcag gcgcagctaa aatcatcttt aactgaaaca aaagagaagt
    82321 tagaaatgat ccaacaaaac gggaaaaagt tccaagaagc attagcaaaa gaacagccta
    82381 aaatgtatca attaatgaat caagtccagc agctacgtgc tcgtaagaaa agccttcaag
    82441 aaatccaaga aaactatttt ggtttttacc aaggggttcg attggtcttg cagcataaac
    82501 agcagctttc gggtattgtc ggagcagtgg ctgaactgat cgatgttcct gcagatttca
    82561 cgttagctat cgaaacagct ttaggtgggg cagcccagca tgtcatcgtt gaaaatgaaa
    82621 aagatgctcg gcaagccatc acgtatttga aacagcaaag aggaggaaga gcaacattcc
    82681 ttccactgac gacaatcaaa ccaagacaat taccggctca tatattgaca caagcagcag
    82741 ctgtagaagg gtttataggg attgccagtg aacaagtcag ttatcccgat caaatccaaa
    82801 ctgtggttca aaacctgcta ggaacgatcc ttttagccaa agacttaact agtgccaatg
    82861 caatcgcgca aacgatccgc tatcaatatc gagtggtttc cttagaaggt gatgtgatga
    82921 atgccggagg atccatgaca ggtggagcga ataaacgagg aaaccaaggc agtttgtttg
    82981 tacaaaatca agaattgaaa cagttgacta gtgaatttga agaagcagat aaacaactcc
    83041 aagcacaaga aaaaaaagtt caggagctgc aacaagaaac agctcgttta gccgaagaac
    83101 aagaagtttt gcgtacacga ggcgaacaat tacgttttga agaacaagaa gcgacaaatc
    83161 agctacaaaa catcataaat gaacttgaac gttttgaaaa agaaaagcaa atctcaactt
    83221 ttgagacacg tgaactgcag cagtttatcg aagattacga aaagcaaacg aacgaattga
    83281 aagacaaaca aacagatctt gaatcgcaac gacaaaaaat cgacgaagaa atcaaatcgt
    83341 taagtcaaga aagcgaccag atggaagcca gaagagcaca agtacaaagt caaaaagcac
    83401 aagaacaagc agatcttgct gtcttgaaag agcagtttaa tcatctgcag atccagctcc
    83461 gcggtgcccg agtccaaaaa gcagaggcaa ccgaacgtca agaagcgata gaaaagcagt
    83521 tggcgacatt gacagcagac ttttctgatc atgaagtcac agaagaaagt ttggaaaaac
    83581 aaattaacga attatctgct cagcgagaaa cattaaaagc cgagttggca aaagccaaag
    83641 agcagcgaga tagaaggcaa aaggagatcg ataagcttga agcagtcttg gcagagcgta
    83701 atcgtgagca aaaagcacga ctgtcggaac aatccaaatt agaagtccaa aaagaccgag
    83761 ctgaaatgat tttagataat catttgacct atctgcagtc agaatatcag ctgacatttg
    83821 agaaagcttc ccaagactat caagaaacaa ccgatatcga agacagtaga acaaaggttt
    83881 catccttaaa agaacagatt gaaaaactgg gaccagtaaa cttaaattca atcgaacaat
    83941 acgaacaagt cagtgaaaga catacattct tagcaacaca gcgagatgat ctgttagcag
    84001 cgaaaaacca attgtttgaa acgatggatg agatggatga tgaagtgcgg acaagattca
    84061 aagaagtatt tgaagctatc cgtcaagaat tcaaaattgt ctttccaaat atgtttggcg
    84121 gcggtaaagc tgaattagta ctgacggaac caactgattt gctgaaaaca ggtattgaga
    84181 ttgaagtaca acctcctggc aaaaaactgc aaagtctaag tttgctttct ggaggagaac
    84241 gagcacttac agccatcgcg ttgctctttt ccattatccg tgtctgtcct gttccgtttt
    84301 gtatcttaga tgaggtagaa gcagcgttag atgaagccaa cgtgaaacgt tttggtcgtt
    84361 acttgagcga tttccaagat gacacacaat ttatcgtagt cacacaccgc aaaggaacga
    84421 tggtcgcagc agatgtactg tacggagtga cgatgcagga atccggtgta tcaaaaatcg
    84481 tttctgtacg gatggaagat atcaatgaag aggcgaaaat caacgaaacg aaagtgtagg
    84541 aacgaaaatg atcaaattag tagcaatcga tttggacggt acattattaa atgaacaaaa
    84601 gctgatttca gatgagaata aacaagcatt agcacaagca aaacagcaag gggtcaagat
    84661 cgttctatgt acaggacgcc ccttagctgc gatggcgcat tatctgcaag aattaggact
    84721 ggtagatgaa ggtgactaca gcatcacttt caacggcggt ttggtacaaa aaaatgatac
    84781 tggtgaaatc atggaaaaga aagtgatgga agtatcagat atccaacgct tatacaaact
    84841 ggctcagcaa ttggatctgc cactagatgt tttgtctgac aatgtggttt tacagctgcc
    84901 tagtgcccca aggaagcaat ccttgtacaa tgtgttgaat aagctgctgc aattccaacc
    84961 agcggatata agcgatatta cagatgaatt tgtgcttaat aaggcagtaa tcgcttatga
    85021 ccaagaagaa ttagatccta aaatcaaaga aataccagca gaattccatg aaatgtatga
    85081 gatcatcaaa acaagaagta tgcttttgga atttatgcca aaaggtgtta caaaagctta
    85141 tggtatttct ttattggcaa aagatcttgg tttgaaacaa gaagaaatca tggcgatcgg
    85201 tgatgaggaa aatgatttac cgatgatcca atatgctgga ctaggcgtag cgatggagaa
    85261 tgctgtgcct ttcgtcaaag aagcagcaga tttcgtgaca gccagcaatg aagaaaatgg
    85321 cgtagccaaa gtgatccaaa aatttgtatt agagtagggg gagtcaaatg ggattttttg
    85381 ataaaatcaa gcgtgctttt actggcgaag agaaaacaga agaagttgaa gtacaggaga
    85441 agtacgacaa gggtttagaa aagacgcgta aaacatttgg tgaacggatg aatgagttgt
    85501 ttgccaattt tcgaacagtc gatgaagact tttttgaaga attagaagaa acacttatcg
    85561 gtgcagatgt aggttttgaa acagcgatac gaattaccga agcattacgt caagaagtga
    85621 aactcagaaa tgcgaaaaaa tcggcagaag tccaaaatgc gatcatcgag aaacttgtgg
    85681 atctctatga agcggaagga atcaatgaag taaatgaact aaatatccaa gaagacggac
    85741 taacagttat tttgtttgtc ggtgttaatg gtgtagggaa aacaacaagt attggtaaac
    85801 ttgcccatca gttcaaaaac gaaggtaaaa aagtattgat ggcagcagca gataccttcc
    85861 gcgctggagc aatcgaccag ttagttgtct ggggcgaacg cgctggtgtt gaagttgttc
    85921 gtggcaatgc aggaggcgat ccagcagctg tcgtattcga tgctttggaa agagcaaaga
    85981 cagagcaggc agatgtttta ctagttgata ctgcaggccg actgcaaaat aaagtgaact
    86041 tgatgaacga attagaaaaa ataaaacgag taatccagcg ggaattacca gaagcacccc
    86101 aagaaacact tcttgtatta gatgcaacca ctggtcaaaa cgcgatggtt caagcaaaac
    86161 aattcaaaga aacaacagat gtgaccggtc ttgttttgac aaaattagat ggtacagcaa
    86221 agggcggtat cgtacttgct atccgtaatg aattgcattt accagtgaaa ttggtcggtc
    86281 ttggtgaagg aatcgatgac ttggagccgt ttgatccaaa tgattttgtc gttggattgt
    86341 tcaaaggatt actaaaagaa gaataaccca tgtctaaaaa gcaggagctg tgacaaaagt
    86401 tgcagctcct tgtttgaatg attgttttta acatagctgt gtttttgatg ccttcatttt
    86461 tatacttatt tgtaagcggt tctcaaacaa tttcgttctt tttcttagac tttttgatta
    86521 taattagatt catatgaatt ggaggtcact taatgatgaa ttttaagcaa ttattattgc
    86581 atgtcaatgc gcgccctttt atcgaccaag ctcgatttgg tatagaacgg gaaggacagc
    86641 gggttgatct tgcaggaaat ctagcaaaaa ccgatcatcc agcaatcttt ggcgatcgat
    86701 cctatcaccc ctatatccaa acagatttta gcgaaacaca aacagagatg atcacccctg
    86761 ttaccgattc tattcccgaa ttatttcagt atctcgctgc tgtttatgat gtgactgctc
    86821 gttctatacc gaaagaagag atgatctggc cattaagtat gccacctgcc ttaccggaaa
    86881 aagacgaaga gatcattatt gcaaaattaa aaaatttcga agatgtcttg tatcgacgtt
    86941 atttagcaaa agaatacggg aagcgaaaac agatggtaag cggtattcat ttcaattttg
    87001 aatttggcga tgagttgcta agaacattgt tcagccatca ggaagaattt caagattttt
    87061 ccgaattcaa aacagaactt tatttgaaaa cagcgagaaa ctttatgcgc tatcgctgga
    87121 tgatcactta tttattcggt gcttcaccaa tgagtgagaa gaactacttt ttagatgaat
    87181 cacatccgca agaacctgtt cgcagtattc gcaacagtgc gctgggctat acgaatcatc
    87241 caaatgtgaa agtttcctat gcttctatga aacagtattt agcagatatc gagcgaatga
    87301 tcgaagaagg gaaactttcg gaggagaaag aattttatac accgcttcgt ttccgaggcg
    87361 gaaagaaagt cgcagattta gcaacaacag gtgttcgtta tattgaattg cggaatatcg
    87421 atttgaatcc ttatgcaaga ttgggtatca atccagagca agttcggttc ttacaattat
    87481 tcctgatgta catgttatgg acagaagaaa aagaagactg tgaccaatgg gtagcagaag
    87541 gtacgactcg aaataacaaa gtagcactag aacaaccatc agatcaaaca gaatttcatc
    87601 aagaaggtag agaaattctt gaaggaatga agcaaatgct ggttgaattg gattggttgg
    87661 attctcttta tttagtagag gaagcattga ctcaaatgga tcatcctgaa caaacgttag
    87721 cagcgaaact ctatcaagaa gcacagctat cgagtcagca agaagttgct gtggcattgg
    87781 gacatcaata ttataaagaa agccatgaac gtccctatca gttggccggt tttcgcgaaa
    87841 tggaactttc tactcagata tttatgttcg atgccatcca aaaaggtgtc caagtcaaag
    87901 tattagatga gtcggatcaa tttttgcgtc tgcaattcca agaccatgta gaatatgtga
    87961 aaaatgcaaa catgacaagt aaggacagct atatcgtccc acttatcatg gaaaacaaaa
    88021 cagtaacgaa aaaagtctta aaagaagctg ggttccgggt accaggaggc gcagaatttt
    88081 catccatgga agaagcggta aaagcttatc cgagatttgc cgagcaggca tttgtgatca
    88141 aacctaaatc aaccaattac gggttaggta tcacgatttt caaggaagga gctagtctgg
    88201 aagattatca agcaggttta gccattgctt ttcgtgaaga cagttcggtt ttagtagaag
    88261 aatttatgcc gggaacggaa tatcgtttct ttgtgatcga cggagaagtc caagccatca
    88321 tgttgcgagt ccctgctaat gttattggtg acagtatccg aacagtgaaa gaacttgttg
    88381 aagaaaaaaa tagtgaccct ttgcgtggaa cgaatcatcg tgcaccttta gaattgatcc
    88441 aattaggtga attagaacaa ttgatgttga aagaacaagg gttgacgatc gaatcagttc
    88501 cccaagccaa tcaaattgtt tatctaaggg agaactcaaa tatcagtaca ggtggtgatt
    88561 cgatcgatat gactgatgaa ttttctgaag cttacaaaaa aatcgccgtt tctgcggtag
    88621 aagcactagg agctaagatt agtggtatcg atttgattat accagataaa gaaatcgatc
    88681 ctacaactga taaaaaagca tatgggatca tcgaagcaaa tttcaatcca gccatgcata
    88741 tgcatgttta tccttttgca ggaaaaggca gaagattgac gatgaatgta ttgaaattgt
    88801 tatatcctga agtattttaa aagaaaaagc ttttgacaga ttgtcctatt tgatgtcaat
    88861 ctgatcaaaa gctttttttg catgtaaaaa tttatgtgtt ttttcgtttt aaaataaacg
    88921 atcccagaag ctttttttct tttttggttt ttctttttca gctggcgaag agttttcttc
    88981 tgggtatttt ttttcgatat caatcacagg ttcatttact gcttcttttg ctgttaagag
    89041 caatccgaat tcctcaggat ttgcagtgac gtaatcgttg actacagtga aacggctatt
    89101 gtttttcgtt gctagctgga tatattggtt ttggacagaa aggggtagag ccccattcaa
    89161 gagtatggaa gcatctggat gttttttgag ttcttttaaa aagttttctt tgttttttgg
    89221 ttggatcatt tcagcaacag tcatactaag atagcagcgt tccctgaatg tccctaaata
    89281 tttatgctgt tcttcagggt tcactaaagg tgtaccataa agtcccttat ccaaatggct
    89341 ttttacatca tcttgtgcca taaaaaatac cctccttctt ctctttgtct ttcttacgat
    89401 tatagcatat tcccaataat acctaaaaca tatgaaaaag cggttgcttt tgttcaacga
    89461 ttcacttaaa ataagaatga aaatcattct caattagaaa ggtggattgg atgcgctacg
    89521 gacgactagt agcattatta gttattttaa gtagtatttc attgttcatc ggagtatcga
    89581 atgtacaatt gtcagaactg ctccagttag atgggcaaag cttacaaatc ttgctagtga
    89641 ctcgtttgcc acgaacgatt tgtttgatcc ttgtgggagc gaccagtagt atttgtgggt
    89701 tgatcatgca acatttgacc caaaataaat ttgtttcacc aacgactgcg ggcacgatgg
    89761 atagtgcgcg tttaggtatt ttagtggcta tgattttcct acctggtgca tccttgattg
    89821 tccgatcctt gacagcattt tgctttgctt ttgcaggaac attgcttttt ttatccctta
    89881 cgcgattgtt cctccagaaa aatcaattga tgcttccgtt gattggagtc atgttaggga
    89941 atatcattgg ttcagttgct actttttttg cttatcaatt ccagttggta caaaatatga
    90001 cttcatggct tcaagggaat tttgcgactg tgatacgagg aaattatgag ttattgtatg
    90061 ctgttgttcc attgttgctc ttggtttggc tatttgctta ccgatttacg atcattggtc
    90121 tgggcgaatc gtttgcagta aatctgggaa tagattacca gaagatgcag tttttaggca
    90181 ttggcttagt atcattggct agtgcagtca tgttgatcat ggtaggaagc attcctttta
    90241 tcggagtgat cattccgaat cttgtctcta gaatatatgg agatcaagtg cacaaaacaa
    90301 ttggcgtaac tgctttagca ggtagtttgt tcttagtatt ttgcgatctt gtcgctcgcg
    90361 tgttgatttt tccttatgaa ataccagtca gtgtgatcgt tggaatcatc ggtggggcta
    90421 tcttccttta tcttctgata aggggacgac gatatgctta aaaaaaagcc atctattttt
    90481 attggtatgc ttgttcttgt tttatttgtt acaggaatgt acttgacgat ccatacatat
    90541 ggaaactggc attttgcatg tgcattgagg agtaagaaaa tttttgcatt tatccttgtc
    90601 tcgctagctg tcagcagtgc cacaatcagc tttcagacaa ttacgcataa ccagtttttg
    90661 actccttcag tattgggact tgagtcgtta tatgtccttg tccaaacggt ccttttcttc
    90721 tttatcggag gagtgacgat gcttcagcag acaggtacag tttttttctt attgaacgtt
    90781 gggctgatgg tcgggctgag tctattgtta tttttctttc ttttgaaaaa aatgagagcg
    90841 aatctttttt tattactgat gtcagggatg attttaggta cgctttttgg tagtgtcagt
    90901 acctttttac aagtcatcat ggatccgaat gaatatgatt tgcttcaagg cagattattt
    90961 gcaagctttg gcaatatcca gagtcagtat cttttgcctg cttctttgat cattatcggc
    91021 tgctggtacc tgttatttcg atatcgacat gtgctagacg ttttccatct cggaaatgaa
    91081 caagcagtaa acctcgggat agacacagat cgttttgaat ttttgatttt atgttgtgtc
    91141 agtcttttga cagggacatc aacggctctt gtgggtccag ttactttttt agggtttatt
    91201 gtcgcaaata tcagttatcg tgtcttttct acttatcgtc atggtcagct atttatcgga
    91261 agctttctgg tagctatgct gtttctgaca ggaggacagc tcgcagtaga gcagattttc
    91321 ggctggaata caacagtgag tgtcgtcgtt gaatttgtcg gaggaattta ttttgtcaca
    91381 aaattgattc gcgaaaggaa gagtatccta tgattgattt acaaaaggtc aataaactgt
    91441 accatggaaa atcagtggtt aaagatgtac agttaacgat tcctgaaaag aaattgactg
    91501 cttttatcgg tccaaatggt gctggaaagt caacggtgtt gtcgatgatc agtcgtttga
    91561 taccaaaaga tacaggtgaa atctatttag accataacga agtaaaggct tggaaatcca
    91621 atgaattagc caaaaaatta tcgatcctac gacaatcgaa tgaaataaat gtccaaatca
    91681 ccgtgaaaga attagtagaa tttggacgat ttccttatac gaaaggaaaa ttgacgacaa
    91741 aagacgagga aatcgttcag gaatcactgg aacaccttgg gttagaagaa atagcggatc
    91801 aatcgattga cactctttca ggcggtcaac tccaacgtgc gtatattgca atggtgctag
    91861 cgcaagacac ggattatatt ttactggatg aacctttgaa caatctagat atgaattttg
    91921 cggttcaaat catgcagatt ttaaaaaatt tagtacaaga aatcgggaaa acaattgtta
    91981 ttgttcttca tgatatcaac tttgctgcaa gttatgcaga cgaaattgtt gcaatgaaag
    92041 atggacagtt atttacgcaa ggaacgaccc aagaaatgat aaaaaaagat gtattggatg
    92101 aattatacga aatggacata cggatttgtg agattgaagg ccgcagattc tgtctttatt
    92161 tctaaaacta acattaggag agaagagatg aaaaaattag tagtaggtat cttgattcta
    92221 gcatcatttg gtttagcagc atgtggaaat agcaacaaca ccagtcaggc agacacaaaa
    92281 aaaagcagca cacaaactga aactacattg acgataacag atagtaatgg agaccaaatc
    92341 gaggtgccaa ataatccaga aaaagtcgtc gtattcgaca atggttcttt agatacgatg
    92401 gatgctcttg gtgttggaga taaggtagtt ggagcagcta caagcagcct tcctgagtat
    92461 ctttcatcct ataaaaaagt agaatcagca ggtggaataa aagaaccaga cttagaaaaa
    92521 attaatcagc ttcagccaga tctaatcatc atttctggcc gacaaagaga ttttcaaaaa
    92581 gatctaagtc aaattgctcc gaccattttc ctttctttag atgcgaaaaa tccatgggaa
    92641 tctttccagc aaaatgtcac tgctttaggg gagatctttg gtaagcaaga agaagctaaa
    92701 actcaattag aggaattatc tagtgcaatc gatcagacga aaaagaaagc agaagctact
    92761 gacaaaaaag cactagtcac acttgtgaat gaagggcaat tatcagccta cggatctggt
    92821 tctagattcg gtttcatcca tgatttattt ggctttgaac aagcagatga tcaaatcgaa
    92881 gcatccactc acggtcaaag tgtttcttat gaatatgtgc tggaaaaaaa tccggatatc
    92941 ttatttgtag tagacagaac aaaagctatt ggtggagacg acagcaaaga tgatatttct
    93001 gccaatgaac tggtagcaca aacgaatgct ggtaaaaatc agcagatcat ctcccttgaa
    93061 ccagatgtct ggtatttaag cgggggcggt ttagaatcaa tgaaactgat gatcgaagat
    93121 gtgaatcaag catttaaata gcataaataa cagaggatta aactctcata ctatgtaaca
    93181 ataatgtttc ttacattctt aaatagcaag agcctatcct caaagaagtt atcaaaaacc
    93241 atccgtaaaa tcgtattgat gattttacgg atggttttta tttatttgag aaatcttttt
    93301 tcaacgtgaa ttcattatct acatcatacg ggttgattct agaatgggga gtggcaaaaa
    93361 atagtgtgat ccattttttc agcatcaaga tcgcctcctt tgatttgaat caaaggtaca
    93421 aagcgaaact tagataaaac ttaaatttag atatttttat tttgtaccga tctcttcttt
    93481 aaacgatgtt ccatctgctg cttctcggca ataagtccac tagactagaa aatatgagaa
    93541 gctattttct gtccagcgtt cttattactc aaagcaaatc gcagttgtca caatctcttc
    93601 taaacgatgt tccatctgct gcttctcggc aataagccca ctagactaga aaatatgaga
    93661 agctattttc tgtccagcgt tcttattact caaagcaaat cgcagttgtc acaatctctt
    93721 ctaaacaatg ttcaatcagc tgaccttcgg tattagctca aagcactgat ttcacaacct
    93781 ctatttaggc atgtgtctca gcaagcggtt gatagttgat gatctgcatt tttcgataaa
    93841 aatcggcggg aagcttactc caccgtttta ataattgata gaaataactc tcttctgctt
    93901 tgattttata ctcgcttaat atcaatccaa aactattcgt tttttttgaa gctaattttt
    93961 tataaatatc caatgctttg gttttatctt cattgacaaa gagtgttttt acttcaatat
    94021 aagaaccctc gatattttgt gtttcttcga taaaatagat gttttttata tattccttca
    94081 tagtgattcc tccaaagaag tcttttattt gataagttta ctctaccgaa gagttgttaa
    94141 taatccgtga acaaaaagta attctttttc caatagttgt taaaggtttt ttatttttta
    94201 tagtattttt tgttatttaa gaaaggaata aggttaaaca aataacaggt tgctttttgg
    94261 gtctttttcc gttcaagaaa ctgaacgaag aaaagggaaa gttgtctctt ttgtgataag
    94321 agaaatcata ataaacgaat gacttacttt ccaataaatt gagaacagtc agaaccagac
    94381 aaattagttg catctgatga ataaagtgat tattctatga catgaggtga tacagatgaa
    94441 attagaagtg acaaaagaag cacaagaagt attgaagaat aaattagaag cagacgatca
    94501 acttttatta gatatcgaaa acggtgacgg tccatttgca gaaagccaag tcagttgcca
    94561 attggatacg gcatttcgat tgatcattgt caaaaaagat acacaaacag atctaagtct
    94621 ttattctgta gtggctgata caccagttgg accaatcaaa atcaaagaca gtgcaatact
    94681 ttacatggat gatccatcca cacttgcttt agaaccaact tataatagtt tacaattaaa
    94741 aggaccaagc ggcttgagaa aaggcaactt gcaagttgtt cgaaaagact agagggataa
    94801 aatgggaaaa ttggcgatca tcagcgacct acatgtggac atcaataaat tcggcgaatc
    94861 agagctgatg cagctttggc aagttttgca aaagcacaag atcactcggc ttcatctagc
    94921 cggagatacg gctaataaag tagaccgttg cctagctgtt gtcgaatttt tcgtcaataa
    94981 aggattatct acgacatttc atttcggcaa tcacgagttg gcagatgtgc ccaatgaaca
    95041 gatgatggac cattttccag acccacattt tttaaatgaa cggtatattg ctttaaatga
    95101 gcagaccgtt cttttaggga tgaatggctg gtacgactat cagttttccg aattgaaaga
    95161 ttacgaggca atcgtacgaa tgaagcgttt gtattggtat gatcggttca tccagcgtgg
    95221 gaaaacagat ctcgaggtca atgcagaaat gcttgcagct gcaaaaaatt tgctagacca
    95281 gctggaagca aaaaaacttg aggtgatttt atccactcat ttcgttccta aacgggcatt
    95341 tatcatgtat caatctgctc cttatgagcg ttggaacaaa ttaaatgcat ttttaggatc
    95401 agaatcattc ggagaattac ttgaccgtta ttcaaatatc aaacaagtag tttttggcca
    95461 tacgcatcgt cgctttgaag ataaatccat cggtcacacg atttacagct gcagaccttt
    95521 tggttattat tatgaatggc agctgacccg agattttgtc ttgaaagaac agttgctaga
    95581 cagctatgat cctatgaaat tacgtgtcct cttgcgtgcc catcacccag cttttcaggc
    95641 ctatcaaaca caacatttgc aagaggaatt tgaacaagcg atgacaatcg ttccgtattg
    95701 agacagcatt agtaatgtgt aaaagaaaat accttgtaaa acataaggat aagtaagagc
    95761 agtagatcca tttatcgtgg atatgctgct ttttttgcgg aaaataatca gcgaatgttt
    95821 atttgatcgg atgatttgta actcgcagag tactagatgc aagcgtgtat tctttttttg
    95881 aacgataatc acaaaaaaaa atata
//
LOCUS       DAYYDS010000002        89653 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000002 DAYYDS010000000
VERSION     DAYYDS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.002"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..2165)
                     /locus_tag="ACHA02_000108"
     CDS             complement(552..2165)
                     /locus_tag="ACHA02_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8668497.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="ACHA02_000109"
     CDS             complement(2306..3214)
                     /locus_tag="ACHA02_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8668498.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3397..4521
                     /locus_tag="ACHA02_000110"
     CDS             3397..4521
                     /locus_tag="ACHA02_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8668499.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4748..5665
                     /locus_tag="ACHA02_000111"
     CDS             4748..5665
                     /locus_tag="ACHA02_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8668500.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5658..6491
                     /locus_tag="ACHA02_000112"
     CDS             5658..6491
                     /locus_tag="ACHA02_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8668501.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6574..7692
                     /locus_tag="ACHA02_000113"
     CDS             6574..7692
                     /locus_tag="ACHA02_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8668502.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7713..9584
                     /locus_tag="ACHA02_000114"
     CDS             7713..9584
                     /locus_tag="ACHA02_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8668503.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            9578..10801
                     /locus_tag="ACHA02_000115"
     CDS             9578..10801
                     /locus_tag="ACHA02_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8668504.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10887..12821)
                     /locus_tag="ACHA02_000116"
     CDS             complement(10887..12821)
                     /locus_tag="ACHA02_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8668505.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13047..13703
                     /locus_tag="ACHA02_000117"
     CDS             13047..13703
                     /locus_tag="ACHA02_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8668506.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13777..14130)
                     /locus_tag="ACHA02_000118"
     CDS             complement(13777..14130)
                     /locus_tag="ACHA02_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8668507.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14322..15251
                     /locus_tag="ACHA02_000119"
     CDS             14322..15251
                     /locus_tag="ACHA02_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8668508.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15262..16098
                     /locus_tag="ACHA02_000120"
     CDS             15262..16098
                     /locus_tag="ACHA02_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8668509.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16346..17122
                     /locus_tag="ACHA02_000121"
     CDS             16346..17122
                     /locus_tag="ACHA02_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8668510.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17298..18041
                     /locus_tag="ACHA02_000122"
     CDS             17298..18041
                     /locus_tag="ACHA02_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8668511.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18034..19614
                     /locus_tag="ACHA02_000123"
     CDS             18034..19614
                     /locus_tag="ACHA02_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8668512.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19865..20344)
                     /locus_tag="ACHA02_000124"
     CDS             complement(19865..20344)
                     /locus_tag="ACHA02_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8668513.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20360..21112)
                     /locus_tag="ACHA02_000125"
     CDS             complement(20360..21112)
                     /locus_tag="ACHA02_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8668514.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21229..21299
                     /locus_tag="ACHA02_000126"
     tRNA            21229..21299
                     /locus_tag="ACHA02_000126"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21261..21263,aa:Gly,seq:ccc)
     gene            21660..22385
                     /locus_tag="ACHA02_000127"
     CDS             21660..22385
                     /locus_tag="ACHA02_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8668515.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22640..22906
                     /locus_tag="ACHA02_000128"
     CDS             22640..22906
                     /locus_tag="ACHA02_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8668516.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22903..23334
                     /gene="ruvX"
                     /locus_tag="ACHA02_000129"
     CDS             22903..23334
                     /gene="ruvX"
                     /locus_tag="ACHA02_000129"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8668517.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23359..23664
                     /locus_tag="ACHA02_000130"
     CDS             23359..23664
                     /locus_tag="ACHA02_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8668518.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23745..24890)
                     /locus_tag="ACHA02_000131"
     CDS             complement(23745..24890)
                     /locus_tag="ACHA02_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8668519.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24951..25598)
                     /locus_tag="ACHA02_000132"
     CDS             complement(24951..25598)
                     /locus_tag="ACHA02_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8668520.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25792..26085
                     /locus_tag="ACHA02_000133"
     CDS             25792..26085
                     /locus_tag="ACHA02_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668521.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26129..26440
                     /locus_tag="ACHA02_000134"
     CDS             26129..26440
                     /locus_tag="ACHA02_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668522.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26437..27061
                     /locus_tag="ACHA02_000135"
                     /pseudo
     CDS             26437..27061
                     /locus_tag="ACHA02_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27202..27360
                     /locus_tag="ACHA02_000136"
     CDS             27202..27360
                     /locus_tag="ACHA02_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668523.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27361..27723
                     /locus_tag="ACHA02_000137"
     CDS             27361..27723
                     /locus_tag="ACHA02_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668524.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27760..28608
                     /locus_tag="ACHA02_000138"
     CDS             27760..28608
                     /locus_tag="ACHA02_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8668525.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28598..30073
                     /locus_tag="ACHA02_000139"
     CDS             28598..30073
                     /locus_tag="ACHA02_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8668526.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30339..30746
                     /locus_tag="ACHA02_000140"
     CDS             30339..30746
                     /locus_tag="ACHA02_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8668527.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30749..30964
                     /locus_tag="ACHA02_000141"
     CDS             30749..30964
                     /locus_tag="ACHA02_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668528.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30968..31348
                     /locus_tag="ACHA02_000142"
     CDS             30968..31348
                     /locus_tag="ACHA02_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8668529.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31482..31637
                     /locus_tag="ACHA02_000143"
     CDS             31482..31637
                     /locus_tag="ACHA02_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8668530.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31706..32179
                     /locus_tag="ACHA02_000144"
     CDS             31706..32179
                     /locus_tag="ACHA02_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8668531.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32176..33870
                     /locus_tag="ACHA02_000145"
     CDS             32176..33870
                     /locus_tag="ACHA02_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8668532.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33836..34021
                     /locus_tag="ACHA02_000146"
     CDS             33836..34021
                     /locus_tag="ACHA02_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668533.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34025..35200
                     /locus_tag="ACHA02_000147"
     CDS             34025..35200
                     /locus_tag="ACHA02_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8668534.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35193..36716
                     /locus_tag="ACHA02_000148"
     CDS             35193..36716
                     /locus_tag="ACHA02_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8668535.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36772..37056
                     /locus_tag="ACHA02_000149"
     CDS             36772..37056
                     /locus_tag="ACHA02_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8668536.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37043..37378
                     /locus_tag="ACHA02_000150"
     CDS             37043..37378
                     /locus_tag="ACHA02_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8668537.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37673..38299
                     /locus_tag="ACHA02_000151"
     CDS             37673..38299
                     /locus_tag="ACHA02_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668538.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39041..39878
                     /locus_tag="ACHA02_000152"
                     /pseudo
     CDS             39041..39878
                     /locus_tag="ACHA02_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            39943..40377
                     /locus_tag="ACHA02_000153"
     CDS             39943..40377
                     /locus_tag="ACHA02_000153"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8668539.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(40445..41767)
                     /locus_tag="ACHA02_000154"
     CDS             complement(40445..41767)
                     /locus_tag="ACHA02_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8668540.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41921..43561)
                     /locus_tag="ACHA02_000155"
     CDS             complement(41921..43561)
                     /locus_tag="ACHA02_000155"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8668541.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43891..44361
                     /locus_tag="ACHA02_000156"
     CDS             43891..44361
                     /locus_tag="ACHA02_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8668542.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            44990..46093
                     /locus_tag="ACHA02_000157"
     CDS             44990..46093
                     /locus_tag="ACHA02_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8668543.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            46377..47057
                     /locus_tag="ACHA02_000158"
     CDS             46377..47057
                     /locus_tag="ACHA02_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8668544.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            47147..48181
                     /locus_tag="ACHA02_000159"
     CDS             47147..48181
                     /locus_tag="ACHA02_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8668545.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            48195..48530
                     /locus_tag="ACHA02_000160"
     CDS             48195..48530
                     /locus_tag="ACHA02_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668546.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48514..50994
                     /locus_tag="ACHA02_000161"
     CDS             48514..50994
                     /locus_tag="ACHA02_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668547.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            51050..52609
                     /locus_tag="ACHA02_000162"
     CDS             51050..52609
                     /locus_tag="ACHA02_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8668548.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52606..53970
                     /locus_tag="ACHA02_000163"
     CDS             52606..53970
                     /locus_tag="ACHA02_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8668549.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            54141..55211
                     /locus_tag="ACHA02_000164"
     CDS             54141..55211
                     /locus_tag="ACHA02_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8668550.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            55226..55894
                     /locus_tag="ACHA02_000165"
     CDS             55226..55894
                     /locus_tag="ACHA02_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8668551.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55914..56492
                     /locus_tag="ACHA02_000166"
     CDS             55914..56492
                     /locus_tag="ACHA02_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8668552.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56895..57806
                     /locus_tag="ACHA02_000167"
     CDS             56895..57806
                     /locus_tag="ACHA02_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8668553.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(58062..58979)
                     /gene="rnhC"
                     /locus_tag="ACHA02_000168"
     CDS             complement(58062..58979)
                     /gene="rnhC"
                     /locus_tag="ACHA02_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8668554.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            59181..59618
                     /gene="zapA"
                     /locus_tag="ACHA02_000169"
     CDS             59181..59618
                     /gene="zapA"
                     /locus_tag="ACHA02_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8668555.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59676..60223
                     /locus_tag="ACHA02_000170"
                     /pseudo
     CDS             59676..60223
                     /locus_tag="ACHA02_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            60338..62698
                     /locus_tag="ACHA02_000171"
     CDS             60338..62698
                     /locus_tag="ACHA02_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8668556.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62785..63099
                     /gene="trxA"
                     /locus_tag="ACHA02_000172"
     CDS             62785..63099
                     /gene="trxA"
                     /locus_tag="ACHA02_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8668557.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            63281..65059
                     /gene="uvrC"
                     /locus_tag="ACHA02_000173"
     CDS             63281..65059
                     /gene="uvrC"
                     /locus_tag="ACHA02_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8668558.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            65204..66103
                     /locus_tag="ACHA02_000174"
     CDS             65204..66103
                     /locus_tag="ACHA02_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8668559.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            66096..67358
                     /locus_tag="ACHA02_000175"
     CDS             66096..67358
                     /locus_tag="ACHA02_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8668560.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67521..69419
                     /locus_tag="ACHA02_000176"
     CDS             67521..69419
                     /locus_tag="ACHA02_000176"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8668561.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(69718..70437)
                     /locus_tag="ACHA02_000177"
     CDS             complement(69718..70437)
                     /locus_tag="ACHA02_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8668562.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    70627..70674
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            70774..71106
                     /locus_tag="ACHA02_000178"
     CDS             70774..71106
                     /locus_tag="ACHA02_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668563.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            71093..73096
                     /locus_tag="ACHA02_000179"
     CDS             71093..73096
                     /locus_tag="ACHA02_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8668564.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            73115..73585
                     /locus_tag="ACHA02_000180"
     CDS             73115..73585
                     /locus_tag="ACHA02_000180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8668565.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73606..74190
                     /locus_tag="ACHA02_000181"
     CDS             73606..74190
                     /locus_tag="ACHA02_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFM8668566.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            74205..75191
                     /locus_tag="ACHA02_000182"
     CDS             74205..75191
                     /locus_tag="ACHA02_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8668567.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            75181..75492
                     /locus_tag="ACHA02_000183"
     CDS             75181..75492
                     /locus_tag="ACHA02_000183"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8668568.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75514..77295
                     /locus_tag="ACHA02_000184"
     CDS             75514..77295
                     /locus_tag="ACHA02_000184"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8668569.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77288..78664
                     /locus_tag="ACHA02_000185"
     CDS             77288..78664
                     /locus_tag="ACHA02_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8668570.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78671..79306
                     /locus_tag="ACHA02_000186"
     CDS             78671..79306
                     /locus_tag="ACHA02_000186"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8668571.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79336..80691
                     /locus_tag="ACHA02_000187"
     CDS             79336..80691
                     /locus_tag="ACHA02_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8668572.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80883..82277)
                     /locus_tag="ACHA02_000188"
     CDS             complement(80883..82277)
                     /locus_tag="ACHA02_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8668573.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82369..83340
                     /locus_tag="ACHA02_000189"
     CDS             82369..83340
                     /locus_tag="ACHA02_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8668574.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83337..84119
                     /locus_tag="ACHA02_000190"
     CDS             83337..84119
                     /locus_tag="ACHA02_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8668575.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84288..84659)
                     /locus_tag="ACHA02_000191"
     CDS             complement(84288..84659)
                     /locus_tag="ACHA02_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8668576.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84793..85236
                     /locus_tag="ACHA02_000192"
     CDS             84793..85236
                     /locus_tag="ACHA02_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8668577.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85279..86088)
                     /gene="yidA"
                     /locus_tag="ACHA02_000193"
     CDS             complement(85279..86088)
                     /gene="yidA"
                     /locus_tag="ACHA02_000193"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8668578.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(86107..87474)
                     /locus_tag="ACHA02_000194"
     CDS             complement(86107..87474)
                     /locus_tag="ACHA02_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8668579.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87458..88288)
                     /locus_tag="ACHA02_000195"
     CDS             complement(87458..88288)
                     /locus_tag="ACHA02_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8668580.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            88451..88888
                     /locus_tag="ACHA02_000196"
     CDS             88451..88888
                     /locus_tag="ACHA02_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8668581.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88964..89584
                     /gene="rpoE"
                     /locus_tag="ACHA02_000197"
     CDS             88964..89584
                     /gene="rpoE"
                     /locus_tag="ACHA02_000197"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8668582.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        29551 a        15170 c        18472 g        26460 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gacaagtaaa
     3301 atattttttt atataaatat ttgtgttact aattccgtta atatacataa gtgaaagcgt
     3361 tttatataag aacttgaaaa gaaagaaggg atagacatga aaaaaagata caaattggtg
     3421 atagctttgc taaacattac cctattattc agcttaagtg catgtggaaa aactaacgaa
     3481 gaagtaacag caaacatcgc agatccaatg ataaaaaaag ccactacatt agaagtatat
     3541 gtcgcaaacg gcaaactgac aaactatgtt gaaaatgctg caaaaatcta taaccgcaaa
     3601 actggagcaa atattgactt aaaaattgtg aatgtagctt cgggaacagc aacagtacaa
     3661 atgatcaccc caaaattggt aacacacgaa gatatgccgg atattatctc catggcagac
     3721 acttcagcag caggtgtgct ggaaaaattt caagatgctt tttatagtgc tgatgaatat
     3781 ggattttatg acaaatatgg cgatcagttt tatgatcaaa aattgaatat tttgcgtcaa
     3841 caaacgagta atgaaacgat tattccttgg gctagtgatt ttaccccagc actttgttat
     3901 tatcaaccaa aaaaatttgc tgaaataggt gtagattttg aagaaatcag ttcttgggaa
     3961 gaatatatcc aggtggcaaa agcattaaaa gaaaaaactg gtagttttgg aattgccttg
     4021 ccagaatctg gggaccaaga attgtttatc aacatgctag cccagcagaa tcaatctttg
     4081 cttgatgaag aaggaaatat ccgtctgaat actaaagaag cgaaacatgc tgcaaagtta
     4141 atcaaagaaa tgatcgactc agatattgta catttctacg gtgcacaaga tgctgagaaa
     4201 gcatttcaag aatctgcaat gtttgttgct ggcggctggt atgctacgaa tatgtctttg
     4261 aattttcctg atgcatctga caaatggcgc atggctccac tgattccttt ttcaaaagaa
     4321 aatccaggaa aaggtgcagt atcaggagga tcaagttttt atgtccctaa agatgccaaa
     4381 aatccacaag tggcacaaca atttcttaca tttatgctga cagatgaaga atgcttagcc
     4441 aacgcactgg agctgggcgt ggctacatct aactcattgg cctatcaaac agaagcagct
     4501 gagaaaaagt ttgactacta aggaggacaa aaatactatg aactattgaa taaaatcaat
     4561 gaaaatacat caaatactgt tttaccagcc acttattcag atgctacgtc ttatttgact
     4621 accgctgcat ataactattg gcaaacaggt gattttgaaa attcatatct aaaagaagca
     4681 gaaaattttg ctcataagta cggcgtaaaa ttaactggtg attaaaaaat attaggagga
     4741 cttaacgatg atgcaatctt cgttaccaag caagacaata aagaacagca agaaaaaagc
     4801 aacagatacg tctcataaaa aaagtgctta tggattggtc acaccttttg ttattttctt
     4861 tattgtgacg attttgattc ccatcattat ctcgtttgct tttagtttta tgaaaattgg
     4921 ctactcattt gaatttgttg gatttgacaa ttatctagct atcttccgag atccattgtt
     4981 tattaaatct tacacgaatg ttttgattat catggcagga tcaattccaa taacgatgct
     5041 attagctatt tactttgctg ttttattgaa ttcagcacag ttaaaaggaa aaggattttt
     5101 tagaactgtc tattatattc caaccattac atcaacggtg gctgtagctt ccgtattcat
     5161 gacttttttc aatccgactg ggttattcaa tggatttttg aatgcgctag gaattaacgg
     5221 tgtacagtgg ttaacaaatc ctttttggat acgaatatca atgatcatca cgatgatttg
     5281 gcttagtgta ggatataaca ctattttatt tttagctggt cttcaaggga tttctaaaga
     5341 gatatacgaa agtgctgaaa tagatggagc atcgaaattt cggcaattct ttttattaac
     5401 gatccccatg ctaaagccaa tcattttaat ggccacagtt ttagccacta tcaatggctt
     5461 gggtaatttc aatatcccaa atatattctt tgggacttcc aatggaccag aaaattcagc
     5521 tttggtcgta ggagtcaatt tatataaaac aagttttgaa atggttgatt ttggaaaagc
     5581 ctctgctatt gcctggacga tggtgtttgt atctgttata ttatcggcta ttcaatttaa
     5641 attcggaggt gataaaaatg actaaacaaa agatacaaag cagaattttt acgtacgtca
     5701 ttattgtgtt tggtgcctta atatttttga tgccatattt atatatgatt gtagcttcaa
     5761 cgcaaagtaa tcaagaaatt ttaagtggcg gaatcaattt caagtttggg gatatgctat
     5821 taaaaaactg gcaagccatc atggcgagat ataattatcc cttgattatt tggaattcga
     5881 ttgttattgc tgtaatcagt acagttttag ctacagccag ttctacaatg gcgggttatg
     5941 cattagcaaa gtatcgtttt atggggaata aactgttgtt caatacagtt atgctatcta
     6001 ggatggtgcc tttttttacc acattgattc ctttatttta cattatgagt cgtttaggat
     6061 tatcgaatac ttatgcaggt gtgatcatcc cttcgattgc cagtacaact tcagtgttta
     6121 tgatgcgaca atatagcagt caatttccta cagcactgat ggaggctgcc agaatcgatg
     6181 gtgccaatga gtgggtgatt tttacgaaaa tcgctgtacc gattttatca cctacgatta
     6241 ttactagtgg tttacttatc tttatgggaa gttggaatca atatctattt ccattagtca
     6301 tgctttcaga taaagataaa tatacggttc ccttaattat caaaaatatg acggtttcta
     6361 gtacaggtga gccaattaat tatggcgcaa ttatgctaat tttagcaact tcagtgatcc
     6421 caatggtctt gatttatgtg tgggcgcagt ctaaatttaa ggagaatgac attggagctg
     6481 caaataagta aaaaaaataa cgaatgttaa ccatctagtt cgttctagga gcaactccac
     6541 attcataaaa attaaaagaa tggcgggata tccatgaatg aacagacgaa ggaatggtca
     6601 agaaaaattt tgaatgtagc ttctgaaaag atagatgcgg aattagacag aatggggaat
     6661 aaaattccat atatttctta caacggaaaa tatacagacg taataaagga cagaggaatc
     6721 gactggtgga ccaatggttt ttggagtggt attttatggc agctctttca caagacaaaa
     6781 cgagaaaagt tcaaagaggc agcaatacaa caagaacaat tattagaatc tgccctgtac
     6841 catttttcag acgttcatca tgatgtaggt tttttattct taccatcggc tattgcacat
     6901 tatcaacagt ctcaaaacaa acatagtcga caactagggt tacatgcagc tacggtactt
     6961 gctggacgct ataatatttc agggaactat ttagtagcat ggaatgacca taaacctggt
     7021 tggatgatta ttgatagtat gatgaattta ccattactgt attgggcttc gagggaaact
     7081 ggtgatccga ggttctctaa aattgctcgg cagcatgcag atacaacagc taagtatcta
     7141 gtgaggaaag atggatcaac cgggcatatc gcagaatttg atgcagaatc agggaaatat
     7201 attcggttac ttggcgggca aggatacaat gagaattctt gttggagcag aggaaatgca
     7261 tgggctttat atggattcgc gttaagttat cgttacacaa aaaatccgta ctatctagct
     7321 atagcagaaa agatagcagt aaattttatt cagaaaatcg aaaaaactgg ttatattcct
     7381 ctcattgatt ttgctgtaaa agattcgaat attgatacca gtgctggagt atgcgctgca
     7441 tgcggtcttc tggaattagc aggatatgtt tcctctgtac gatcaaaaat gtatcatgaa
     7501 gctgctatca atatactcca aaagacagaa aagcactatg caaattggaa tatggatgaa
     7561 gatgggatca ttggaggcgg agcagaagca taccatagag aactgactta taatgtgcca
     7621 ctgatctatt ctgattattt ttttattgaa ggcttacttc gattagaaca gtcggaatta
     7681 gctatttggt agtaaaaaga ggagaaaata caatgaaaca tccagtacca ctattaagga
     7741 ccagacagga ttactttgac ttatttaaaa agataaatca accactccag ccgttttatc
     7801 gagcaagtgg cgcacaaatt gattatatac atcaaggggt aggatatgga aataaaattg
     7861 caggaatgga aggattctct cgggttttat ggggagcggg gcccgcaatt gaccaattag
     7921 atgaacgatg gttgaaatta attctatcag gaataaaagc tggaacagat ccttcccatc
     7981 ccgattattg gggagaaata catgaaagag accagcgtat ggtagaaatg cccgcaatgt
     8041 tattagcgtt atatcatcat gaacaattat tatggaaaaa attagataca ggagaaaaaa
     8101 gccagattat tcattggttc tcgcaaattt ttgaatatga atgtgcagat ggtaattggc
     8161 aattttttaa gataattgtc ggaacaatta tccaaaaatt tggttatcca gttaaagaat
     8221 cagcactgaa agaagcattt gataatatag aaaattgtta tcttcaaaat ggctggtatc
     8281 gagacagttc aagaggtcgc caagattatt ataatccttt tgcatttcat tattatggtt
     8341 tgatttatag cgtactagaa ccagaaaagc ccattagcca gatatataag aaccgagcta
     8401 ttgatttttc tagagactat attcattttt ttgcggaaga tggtgcaaat gtgccttttg
     8461 gtcgaagcat gatttatcgt tttgcagttt caagcttttg gtcagctatg ttgtgggccg
     8521 atcttttgcc ttttaaatta ggtgaaatca aggggttagt caatcggaat ataagatggt
     8581 ggatgcaaaa agaaatattt gatgaaaaag gtatcctgaa tattggttat acctaccctc
     8641 aattgacatt gactgaaccc tataatagta cgttatctcc attatggctg aataagattt
     8701 ttttattgtt ggcgctacct gaagaccatg agtattggac ggctaaagaa atgcctatgc
     8761 ctattcgtga aggaactaaa cttctttcag aagccaattt gctcatacaa catgataatg
     8821 gacatacatt ttttttgaat gcgggacaat tagggccaaa tttccataca ttgaccaatg
     8881 aaaaatattt aaaatttgct tattctagtc aatttggttt ttcgataccg cgagcgaatc
     8941 aattgaaagc agaaatggca atggattcaa tgctagggat tcagcgagcg gacacatata
     9001 tcacgaccag ttttaagaat gagagcatca aaacctatgg acagttcatc gttcgaaata
     9061 atgtacgaga cattgtatgt aagaaagagg tacttgcctc tacatgggaa cctactttac
     9121 aggcaaaaat cagaacttgg cttattcctt ttgaaggctg gcagattcgc gtacacaaag
     9181 tagaattaga tacagaatat gttctatatg agacagggtt tgctattgaa tgttctcctg
     9241 agaaagaagg aataatcata gaagaagaca aggaaaacta tagggtgagt gaagctggct
     9301 tttcaggtat tatctgttta agtgacgata ctattaaacg aaaatccaca gctattatgg
     9361 gtttgcccaa tacaaacttg atgacatggg aaaacacttt tattcctgga ttagagggta
     9421 catttggaaa aggaacccat tggctcggaa caggagtggt tgctcatcaa gatcgggatt
     9481 atagtttaga agtatggaac aatcgtccca aaattgattt tgatgggaca accgggctaa
     9541 ctatccaaaa taaaaacaat aaaaaaagac taaaattatg ctaaaggctg gtggggaaat
     9601 gcttgataaa gtgatgtttg aagagataat acatccagaa aaatggataa atgaatgtta
     9661 taaagaatta atggaaaaag aatggaataa tgagatcgag cgttgtcgca gacacacgga
     9721 gaggttaatc aaacgtatca gtaacagtca gtatcaattc ccaagcgagt gtcctataaa
     9781 aggacgttat ccattaagag acaatattgg ttggacagaa ggattttgga caggaattaa
     9841 ttggttgtta tttgagaaga cgggtgatcc tttatttcaa caaacagctg aaaaagcaga
     9901 tatagatttt atccacagga ttgactgtca aattatcact aatcatcacg atcttggttt
     9961 tttgtattct ttatcaaccg tagcagatta taaaataacg ggaaatcgaa aaatgaagga
    10021 agcttcaatt gatgctgcag aaacattact tcagcgtttt tgggagaagg caggagttat
    10081 acaagcttgg ggagatccta gtgatccttc acaacaaggt agaatgatca tggattgttg
    10141 catgaatctt ccattattgt attgggcgag tgaagaaact ggaaatcaaa aatataaaaa
    10201 agctgctcag gctcatattc aaaatgcagc agattattta gtaagagaag atggttcaac
    10261 ttatcataca ttttatatgg atgtcaaaga tggaaaacca agatatggaa gtacgcatca
    10321 aggttatagt gataattcct gttgggctag aggccaagca tgggggattt acggttttgc
    10381 attaagttat cgttatttga aagaacaaaa atattttgat ttaagcaaaa tattagccaa
    10441 ttattttatg aatcggttgc cagaagatac catttgttat tgggatttga tctttgatga
    10501 ttctagcaga gaagaaaaag atacatctag tatggtgatc atggtttgtg gattattaga
    10561 attaatagaa tcaggtctat tagacaaaac agaaaagaac ttataccagt ctgctgtttg
    10621 gaaaattatg cgacaattga aagaaagata tgctacaacg gaagaagaag atggcttttt
    10681 aacacagtct gtttataata agcccaaaaa taatggggta aatacacctt gtatttgggg
    10741 tgattactat tatttagaag ctttagtgag aatgactagt aattggtctt cttattggtg
    10801 aaaaaggtaa atatggaatt tttgtttgat aaaataaaaa aagaaaaacc aaaacagcat
    10861 gaatggtttc tgttttggtt ttttcgttaa ttttcatatt cttctaattc caagcttagt
    10921 tcttcccatt ccgtcaattt tgtttcttga tcttgtctag cagcttctaa atcttggttc
    10981 agctcattga gttttacgtg atcttccagg ttttctggcg cgaccatttc ttgttccaac
    11041 tgatcgatcc attcatcgat ccgcgccatt tcttcttcaa cctgagtaat ttttcgttga
    11101 aggctacgga taagcttttg ctgttctttg ctttggtaat aatcagattt gttcgtggga
    11161 gcaggagcag attctggctc atcttgtgcc aacaattcgg cgatttcttc ttcttcctgc
    11221 tttttctcta aataataatc atagtctcct agatagactt tgctgccatt ttcagagagt
    11281 tcgacgactt ttgtcgcgat ccgattgatg aaatatcggt catgggaaac aaaaagcagt
    11341 gtgccgtcat aatcgatcaa tgcattttct aatacttctt tgttgtcaat atctaaatgg
    11401 tttgttggtt catccaaaat caaaaaattt tctttgttca tcgctaactt ggctaaagca
    11461 acccgtgctt tttcaccgcc acttagcaga gggattggtt tttctacatc ttcgccagaa
    11521 aataagaaag cacctaaagc acttcggatc tctttttctg gagtggtagg atgctcatcc
    11581 cacaattctg ctaggatcgt cttgtttgta tgcagatcag cttgaccttg atcgtaatag
    11641 ccgatggtga catttgtccc tagcgtttct tttccacgga taaaagggat ctggtgaatg
    11701 attgatttca gcaacgtcga tttcccgata ccattcggac ctactagagc aatcgcttct
    11761 tgacgacgga tatctagatt gattggttca gataaggtga cattatcata tccgatagcg
    11821 gcatcttcga tttgcaagac tacatttcca gaaggctttt ctatactgaa tagaaagttt
    11881 gcttgttttt catccccttg aggacgatcc agccggtcca ttttttctaa tgtctttctg
    11941 cggctttgcg ctcgttttgt ggtagaggcg cgaacaaggt tccgagcaac gaagtcttct
    12001 aatttatgga tttctgtttg ctgcttttca taagccttcc attcacttgc taactgttct
    12061 gctttcaaat ctagatattt cgaatagtta cctttgtaat ggcgcatttt tcttctgctt
    12121 aattcataga cttcattgac tactttatct aaaaaataac ggtcatggga aacaatcaat
    12181 aatgcaccag tatagctttg cagataattc tcaagccagg aaagtgtctc gatatctaaa
    12241 tggttcgttg gttcatctag aatcaaaatg tcagggtgtt gcaaaagcat gcgagctaaa
    12301 gctaaacgtg tgcgctgtcc gcctgataaa gtatcgattt tctgatcata aaatgaagaa
    12361 tcgaatttaa acccatgcaa tacggaacga atttcatttt cataaccata gccgtttcga
    12421 tctgaaaaat cgtgttgcag ccggtcgtat tcttttaaat aagaactata agcaggtgtg
    12481 tctggcgtac cttcgctaat gaaaatctct agctcgcgca tccgctcttc catttgtcta
    12541 acttgttcaa aagctttcag catctcgttc caaactgttt cattggaatc aagtcctgtg
    12601 tcctgagcga ggtatccgag agtcgctgtt ttatttttag cgatctttcc ttcatcaggt
    12661 gcttcaattc cagcaatgat ctttaataaa gtcgattttc cagcgccatt tctgccaact
    12721 aaagcgacac gagcgcctgt actgatttct aaatgtatgt tttcaaataa gacttctgca
    12781 ccaaaatggc gagcaatctg attggcttgt aataatatca tggaatcctc ttttctactt
    12841 tgtgaattct cctcaaataa aaaactattt ttcaattagt agtctaccat aaattccatt
    12901 atactgcgag atttgcagaa atgataaata aatgaataaa gaataaaatt tcacaatatc
    12961 tgagcaatgg aattgatttt ttcctataaa tactgctatg atggagaaat caaagatgta
    13021 attagacaat acgtggaggt catttcgtga aagatcatat tattccaaag gcgacagcta
    13081 aaagactgcc tttgtattat cgctatttaa ggattttaaa taatgctgga aagaaaaaag
    13141 tgtcttcaac tgaattaagt gaagccgttc aagtagatag tgcaacgatc cgtcgagatt
    13201 tttcttattt tggcgaatta ggaaaacgtg ggtatggtta tgatgtagaa gatcttatgc
    13261 acttttttgc aaagacatta aacgacgatc aattgacaaa tgttgcgctg atcggtgtag
    13321 ggaacttggg aagtgcttta ctaaaataca aattccatca aagtaatagc atacgtatca
    13381 gttgtgcttt tgatgtgaat gaggaaatcg ttgggcgcat cgtagacggt atccccgttt
    13441 atccaatgga agatatgtca gaacaaatca agatccaaca aatcgaagta gcaattttaa
    13501 cgatacccgc tgaaagagca caagaagtag tcaatcagtt aacggaagca ggaatcaaag
    13561 gaatcttgaa ctttaccgct gctcgattga atgctccgtc agatgtattg atccaaaatg
    13621 ttgatttgac gaatgaattg caaaccttga tctatttctt gcattctgac gcaaatgcgc
    13681 attccgaaaa tacagcagaa taaaaataaa aagcgattga acaaatgaag gcaaaacttt
    13741 cacttgttca atcgcttttt tctgtttgga caacgattat tttgttcgga agatttcttc
    13801 ataagaaagg gaatcatctt ctgctgtcag ttcatctagt agataacgga tttttttaaa
    13861 gtcgatgaac cgtgcttgcc gaaagacttc ttttccttga tgaaaaatca ggacaactgg
    13921 cgcagtcagc acttcaaatt tactagctac ttctggtgtt tcaaatgcat ccaaatgaag
    13981 tgccggaata tcataatgtg tgagaagttc ctctagtcgt ggacgaacag cgtgacaaac
    14041 actgcaatta ggcatcgaaa gataaatgac ggcaacggat gactgttgca gtatgtcttg
    14101 aacttcagtc aagttagtgg tttgtttcat ttttattctc ccttttatga tagaagtgat
    14161 atgtatgaga ccttaattta atgggaataa cagaaaaagt caattgaagt gctttggtaa
    14221 aaaataagag ttgtgtaaaa atttacagag caacttgttt tttttgaata aaagcgtata
    14281 tttgcaaagc gaatgaacta gaaataaaaa ggaggaaact catgttttct tttattccaa
    14341 attcggtttt gcagatgagt acgattttct tatcgatcgt tattgaagca ttacctttcg
    14401 tattattagg ctgtcttatc tcaggcgcct tacaagtatt tttgacccca gaacgtgtga
    14461 agagatggct tcctgaaaat cgtatcttct ctatttttac tggttcgatc ttaggatttt
    14521 tctttccttc atgtgaatgc ggcatcgtac cgatcgttca tcaatttgtt aaaaaaggcg
    14581 taccagttca cacagcgttt gcttttatgc tgactgctcc gatcatcaat ccaatcgtga
    14641 ttttttccac ttttatcgca tttggcaatt cttggcagat ggcaagctgg cgggtgatag
    14701 gcagcttttt tgttgccctt gtgattggtg tctggcttgc ctattttcaa aaagacgctg
    14761 tattgaaaga aaaaatcacg tctaaagtag cagaaaatca ccatcatcat gtccatgaag
    14821 aagaacaaag tgagaagaat gaaccaaaaa atcagctgat ccatcaaact gggcatgtgt
    14881 tgacacattc aatcgacgaa ttttttgata caggtcgtta tttgatcatt ggcggattaa
    14941 tcgcagctgc gatgcagact tatttaccaa ctagaattat gctaacgctc ggctcaacaa
    15001 agattttagc cattatcatc atgctgttat tggcctttac gatgtctctt tgctcagaag
    15061 cagatgcgtt tataggatct tctttgttaa gtctgttcgg cacagcacct gtagctgcct
    15121 ttttggtctt tggcccaatg gtcgatatca aaaatctact gatgatgaaa cgatatttta
    15181 caggacgctt tattttatcg ctgatcggac tgattgccct tagcgttatt ggattcacat
    15241 tatttattta ggagggaaaa tatgctgcgg tttttgattt tatctgggta tttcgaattg
    15301 atgatgtact tgcaagtttc tggcaaactc gatcaatata tcaatgtcca ttatcgctac
    15361 ttagctattc tttcaatgat tttatctgct cttttggctt tagttcagct gtatttgtgg
    15421 gtcaaaaacg gaaaagcaga agaaaaggca cacgagcacg atcatgacca tgggttgtca
    15481 aaaccttatc aacgtggaat cgcttatgta ctgctagctt tgccagtaat cgtggggacg
    15541 ttgtttccaa cagttagtct cgacactacg atcgtagaag ccaaaggatt taatttccca
    15601 gtaagtaaag aatcagtagg ggatccggag atgcagacac aatacttaaa gccggatacc
    15661 agtatttatt tcaacaaatc agactactta gaccaaatga atgagttgaa agaaaagtat
    15721 ggggataagc aaactatcaa gatcacagat gaaaactatt tagaagttat ggagctaatt
    15781 tataattacc ctagcgaatt cattggcaaa aaaatcagct atgaaggatt tgtttaccaa
    15841 acaccgaacg atagcaatac agatatcttc ttgttccgat tcggcattat ccattgtgtg
    15901 gcagattcag gagtctttgg tttgttgacg catatgccgg aaggagtaac ggttaaaaat
    15961 gatgaatggt ataaaataga aggaaccatc caatcagact attatcaacc attcaagcgt
    16021 gaaatcccat ctgtagttgt tacaaacgct gagaaagtct ctgctccgaa aaatcagtat
    16081 gtgtatcgtt ctttttaaaa gaacaaaata aataaacata gctcagacag ctgacacgca
    16141 aggacgtcgg ttgtctggct ttttttgcat gatgtattca tttgtttgat atttatttta
    16201 gaagcagcat cctgaaagta tttgatatcg gtttcaaagg tgttggaaaa gattgtgaag
    16261 agaattacaa tttattccta acatgtatgt tgaaaagttg tatgaaatga ttttgatagt
    16321 aaaatatcga gtggaggtac tttttatggt aacgcgtata tttattttgg atgatagttt
    16381 ggtttttgaa aaaatgctag tagaaatttt ggaagaatcc agaattttat taagtccttg
    16441 gaaatttgag atcgttcagg gattgaacgt tatacaattt gttgaattca ttaaaaataa
    16501 tgaaatctat tcctcagata tttttttcct agacatcgat ttgaacgcct attatagcgg
    16561 tatcgaacta ggcgaaatgc ttagagaaaa gaatgaaaag tgtaaaattg tttatctaac
    16621 gtctcatccg aataaagcaa taactgttat taatcgaaag attacgccta gtggttattt
    16681 gataaaagaa ttcacttatg aaaaaaataa aaaaagcatc caaagttact tattgaaaga
    16741 aaaagaaaac tatcctgttc aacatgctac gaaaaaaagg tggacaactg tccgttatgg
    16801 taatagcgat cactatatcc attacgatga aatcctttat ctaatgagca tgccaggtta
    16861 taaaaataaa ataggcctag tattgaaaga tgaagagatt ctcgtaaacg gcacaattaa
    16921 aaaatataaa aaatcactcg aatatgagta tttatgtaaa gatttaaaag cttatatcat
    16981 taatactgaa aacattacat ctattagcag agtaaacgaa atagtaacat ttgcaaatgg
    17041 agaacaacta gagatgggaa agagaagtat tgataaacta aagaatttta tgcaagaaaa
    17101 gatagaaaat gaatcatttt aaagaaaaca aagactcata tcttacctgt tagaagagtg
    17161 ttttcctgta gaagagaaca acccaagtag cagcctagtc gtttctatcc ttttttgtta
    17221 tgcactcttt ttgacattta ctccaccaat aacgaagggt acaataaaaa gtaataacaa
    17281 acaaaaggag tacagaaatg attgaattca tcaatgtaag taaaagttac gggaaaaaac
    17341 aagcattaaa agacttgtct ctctcgataa gacaagggga aatttttgga tttttaggac
    17401 ataatggtgc cggaaaatca actacgatta aaagtttagt tagtattatt gaaccatcat
    17461 cgggaacgat tttagtagat ggaatgaaat tgacggaaaa tcgattgagt atcaaacaaa
    17521 aaatcggtta tgtgcctgat tccccagata tttttttaca attgacggct ggagaatatt
    17581 gggacttgat ttcagctgcc tatgaattag acacacaaaa aaaagaaaag cgtttagcag
    17641 aattgacagc gctttttgat atgtatagtc atcaaaatga aacaatcgca agtttttcac
    17701 atggaatgcg tcaaaaaacg attttgatcg gcacgttgct gccagatcca gatatttggg
    17761 tattggatga acctttgcaa ggacttgatc ctcaagcagc atttgatttg aaagaaatga
    17821 tgaaggctca tgccgcaaaa ggaaaaacag tcattttttc tacacatgta ttggataccg
    17881 cacaacagtt atgcgataaa ctggctattt tgaagaaagg tgaattgatt tatcaggggg
    17941 cagttagtga attattgaat ggctcacctg atgaaacgct agaaaagatt tatttgaaaa
    18001 tggctggaag gcaagcgaca ggagatggac cgtatgtata agcgacaatt attagaatta
    18061 attcgagtta atcttttata tgttaatcca cagctcacga ataaagcaag agagaaagga
    18121 aaaaagggaa agcgattaac tatctatctt ttgtctcaaa atcttttttc agggctgatt
    18181 tttttactga tttacggatt tgctatgttt gctatgaact ttgttaaatt gccaggtttc
    18241 tttacttatt acgtggcact ttttggtatg ttaggtttaa ctcaaagtat atcagtcatt
    18301 tataatatct tttttgaagg aaatgacctg caagtatttt tgcctctgcc ttttggacaa
    18361 gaacaggttt tttcagcaaa gattttggta gttgctttga ccatcattcc ttttgttttt
    18421 ccgcttctag tcatgtttat tttaaccgga tggcgagctg atctagggat tttgcttact
    18481 atcattatct caatcctttt gttttctcta tttttgatta ttcttttttg tctggctagt
    18541 ttgattgttt ttggtttggc tcgaacagca gtgtttaaaa aacatagaaa aacagtttct
    18601 agtatcttgc ttggcagctc gatgctgatc gcatttgctg gaatcatgtg gatgaatcat
    18661 caaacgggga ctattgaaaa tgagttaaca gatcggcatc cgatttcgat attattgcca
    18721 ttctatgatg ctgccagtca gccactttct caacaaggtt tattgggttg gggaatacta
    18781 gctatcacgg ctctgggatt tttccttgct gtaaaacagc tgatagtacc aaagcttaca
    18841 gagcaactga ctgattcaaa cagtgagttc aaaataaaaa gaacccataa aaagaaccaa
    18901 aatgtgcatc agcttttatt tagttacaat gctcaattag taaaagaacc taatttgatt
    18961 atgcaagttc tttcttcttc cttgttaact ccaatcattt ttattcttgc ttttgcccta
    19021 ggaggcgaaa taaaattaac tgatttagat aataagttaa tcggtgttgt tttcctggta
    19081 gggatcgcat tggctttctt aacagttaat cagacttctt ttatcagcaa tcttatttct
    19141 ttggatcaag aaaattttct gtttatccgt tctttgccaa tctctatgaa ccaatattta
    19201 aaagaaaagt tcagattcgg atggatattg cagagttttt taactggtgg gatcgcatta
    19261 ttaagcggac tgtcttttca gctgcctttg ttttttatcc ctagcttgct atttggttct
    19321 ttattcggct gctacttatt gtcactacgt tatttcgcta gagattatcg gttgctgctg
    19381 ctgaactgga ccaatattaa ccaattattt aacagaggtt caggaagttt gggtttagtc
    19441 gcaactatga tgggttcatt gatcatcagt attattctta tcgtcttata cggaatgttg
    19501 gctttgtttt atccattttg gttagtgaat attcctgtga tcggtttagt tctaatactt
    19561 tccgctctat ggatcgttta ttatcaacga acgttttgga aaaaatttga ataaagaaga
    19621 ggggccgtga caaaaatcat gtcgcagccc cttttttaca gatagagggt gttcaaaagt
    19681 cagtttctca gtattaagtc aattttatgt aaaaatgtga gaagctattt ttacaaaact
    19741 ttttttaata ctcataatta cttcttattg ctcctgcggt tacttgtcgc aatgtttgtg
    19801 agatattttg tcatcgctca aggctgaacg tctctttcac aaccttttct ttgttacact
    19861 tcgtttattt aaaaagccgt ttttttcgtt tcatcatgat tgcgtcacgc tttactttga
    19921 aattacggat gttatggtta aagacttctg ataatacaag acctaaagcg attgctccag
    19981 caatcataat tgcttggacc gcataatgag ccgcttctcg ataggattca gtaacgagat
    20041 ttcgaaccgc ttgataagct aaaccaccag ggactagagg aaccatcccg ggaatattga
    20101 aaatcgttac gggcattttc aaacgtttag agaaaagatc gctgatgaat gctactccta
    20161 aagaaccaag caatgaacca aaagcagcag atccgcctat ttgaacacag atccagtaga
    20221 tcatccagcc aaaagcacca gaaagaccac aacaaatcaa tgaacgccta ggtacatttg
    20281 taataacggc aaatgccgca gtcgctaaaa aactaaaaga aaactgaata aatatcgtcc
    20341 ccatacttgt gttcctttcc taataaaaaa gctggaaaat aaaagcaatc gcaaaaccga
    20401 tcatcgtagc agtcatcatc gcttctgttc cgcgagaaac tcctgagaca taatgtccag
    20461 ctagcaaatc acggatagcg ttcgtaattt gaactccagg aactaaaggc atcacacatc
    20521 cgataatgat tagatcctga ttgcttccta agccaaaacg tacgctgtag atagcggcta
    20581 tgccaatcaa taaagaagat aaaaattccg ataaaaattt gattctaaaa aatttcaaac
    20641 tatatagata agctgcgtat cccaatcctc caatgactaa agtcacaggt aaatcggtca
    20701 tctttccgcc aaataacacc ataattgttc cactgatcac tgccgcgcta acgatttgca
    20761 accagacagg aaagaaagaa cggtctcggt cgattgcttg cagttcgcag taaagttctt
    20821 ctaatgttag ctctgaagca acatatttac gtgaaagatc atttactttt actacttttt
    20881 ctaaattaat cgaacggttg gtaatttgtt ccattcggat ggtcgaggta cgatcaagac
    20941 caataaagag accggtttga gtgacatagc tgaccaaacg gaaatttcca gaagctaaag
    21001 caattcgact catcgtatct tctacccgat acatctccgc attgctttcc atcatgattt
    21061 tcccagcgag taaacaagtg tccagcaaca gatctaattc ttttcccgtc atagcatctt
    21121 ctccaataat tgattgatta gttttatttt actacaaaac ataccagaag cgtaaagaat
    21181 aaaaaaactt gaggaaggaa taaaagaggc gtatactgtt tttgtattgc gggtgtagtt
    21241 tagtggtaaa atcccagctt cccaagctga tgtcgcgggt tcgattcccg tcacccgctt
    21301 aaaataggtt gtgaaaaagc tgctctgcaa caagtaataa gaacagccaa gcaaaaacaa
    21361 cttcttatgt ttttaacttg gatggactta ttaccgcaga tgcaagcttt tgcgcaccgt
    21421 ttaccaagcg gttgtgaaat cagtgtattg tcttgagtat gaagaaaatt tttgaataat
    21481 aataaatacc ttacagatag agtggtgact gattagagac gaggaatccg tctctttttt
    21541 gtatttttaa gccatcttaa aacaggtttt attttttgct gtttctattt ctttctagta
    21601 tttatttata ggggaacata tgttaagatt tagggagctg ttagtaaagg agtaaagaaa
    21661 tgagcaaaaa aacaaccaag gcgatcgtgg ggatcgtcgc tgctgtgatt gcaggggcat
    21721 tcggtgtttc caccacacaa aaagattcag atatctttca gcagattaca actatttttg
    21781 attccacacc acaagcttct caagtagcgg atgtgccaga atatgatgga aaacatcaag
    21841 aaatagaaat caatgggaat cagccggaat ttacaaaaga ggatcttagc ctttccaaag
    21901 gaacatggga aagctattca aatattgata aattgaacag agtaggacaa gccaatgcta
    21961 tgcttggaaa agatttgttc cctactgaaa agagagagcc tttgtatatt gacccgacag
    22021 gttggaaaca aaagaaatta agtgacgggc aatggttata taatcgcagc catttaattg
    22081 gtttccagct gactggtcaa aataacaaca ttaaaaactt gatgacaggg acacgttcgt
    22141 tgaatgctcc atacatgctt gctcacgaaa atgatattgc ggcatatatc aaagaaacaa
    22201 atcatcatgt acgctaccgt gtcactcctc attttgaggg gaatgaactg gtagccaggg
    22261 gagtgcaatt ggaagcagaa agcattgaag acaaaaaaat tgaattcaat gtctttatct
    22321 acaatgttca agatggctac acgatcaatt atgagacagg acaagcaaca aaaacaaaaa
    22381 attagtagca gctattcgaa ttttttatga atattatgaa aaaattgtgt aaaaacatac
    22441 attcaaagaa atatagcgta tactttatga aaagagtact tttgataaag ctacagaaaa
    22501 aaggaaaaaa acatacaaat agtgtttctt aatttattcg gaagtttgga atatttgttt
    22561 gtagttttcc ctttcttcga gtaaaataag gttgtagaag gtaagctgga attaccttgc
    22621 gggaaagagg tgtagaatca tgggttttac agatgaaacc gtacgttttg gttttgacga
    22681 tagtcgcaaa aaagaagtga gcgaaacatt agcgattgtt tatcgggctt tggaagaaaa
    22741 aggctacaat ccaatcaatc agattgtcgg ctacttgctt tctggagacc cggcctacat
    22801 tcctcgttat cgtgatgctc gaaatttgat tcgccgtcac gaacgcgatg agatcatgga
    22861 agtaattgtc aaggactacc tctcaaatca tggagtgaat ttatgagagt aatgggcttg
    22921 gatgtcggtt ctaagacagt aggtgttgct atcagtgacc cctttggctg gacagctcaa
    22981 ggagtagaga tcattcggat caatgaagca gaagaagagt ttggctttga tcggttgaag
    23041 gaattagtag cacaatatga agtagaccgg tttgtagttg gattacctaa aaatatgaac
    23101 aattccatcg gaccaagggc agaagcttcc atgtcatacg gagaaaagat caaagaacta
    23161 ttctccttgc cagttgatta tcaagatgaa cgattaacca ctgttcaggc agaacgtatg
    23221 ctagtagagc aggcaaatac ttcaagagcc aaacgaaaaa aagtcatcga taaattagca
    23281 gctgtgatga tcttacaaaa ttatttagac ggctcaggta agcaattatt ttaataataa
    23341 aagaaaagag gtaatcttat gacagaacat aaccattccc acgaccatga aggacacgaa
    23401 cacatcacat tggtggatga ccaaggaaat gaaacattat atgaaatcct actaacagtt
    23461 gacggacaag aagaatttgg acgtaactat gttcttttat atcctgctgg tgtttcagaa
    23521 gatgaagatg tagaattaca agcttatgca tacatcgaaa acgaagatgg aacagaaggc
    23581 gagttagaac aaatcgaaac agacgcagaa tgggacatga ttgaagaagt attcaataca
    23641 ttcatggcgg aagaagaaga atagactata aagctaggag aaaccttgat atgatagggt
    23701 ttctcctttt tttatttttt actgtatgcc atattgtatg ccatctagtc aatatttaag
    23761 tgttgtacta atttttcaac agtcttttct ttcgtttctt ttgccaagtg accgtaacaa
    23821 tcgatcgtct gactaatttt tgtatgccgt aatctagttt gaacttctac catagtagct
    23881 ccagcgtcca agagcattga agcatgagtg tgtcttgcta catgcacacc gagaaatgga
    23941 accatagctt tttcacaaat agtaatcaat cttgcataaa tagcgtggtg tgtgattagt
    24001 tctttataga taccacaaaa aatcatagtc ggatcagtta caccaagtgt cagcatatat
    24061 ttccgctgat taatctgcca tttttttaat atagaaattg ttctttcatc tagtggaaga
    24121 accccagcac tacaatcagt tttaggatca ctgattttat agccattttt ggattggctt
    24181 agtgttttat tgatggtgac cgtattttct tgaaaatcaa tgtctgacca attcaatgca
    24241 agggcttcac tgatcctaac cccgctataa aagagaaagc gtagtaacgt tttatcaaag
    24301 tcgtttcgat aagtttcagg tttgctgttt aggtaatcaa acaaggtgat cagttgttct
    24361 ttactgtaaa ctttgacctt ttctttatgc ttagtggtgg ttcttttagg cattaatacg
    24421 ttgtctagtg gattagtctc aataatccca agattgatag cgtagctaaa aatacgctta
    24481 gaagttgaat gaagtacctt gtattggtca gtagtttcag cccagctatt tacaatttgt
    24541 tgggcttttt ttaccgtgac ttcattcaca cgaagatttc catattttgg aagaatccac
    24601 tttctaaact tgatttcagt tttcatgaag gtggcttctc ggacagtggt tttatatgtt
    24661 tctagccaca agtcataaac ttctttaaac gtttcgttgt tcgctttttt aataccgttt
    24721 ttatcgtagt ccacttgcaa gcgtgacaag gcaagctggg cttctttttt tgttttgtaa
    24781 cctcgtctac gaaccttatc aggtttgcca gtcattttgt tcattccaag ataggcttca
    24841 tagagccaaa atttaccttt tttagtttca tattgcttga atgttgccat aatagttttc
    24901 cctccatagt gtagcaagcc gtgggggcgg ttggaggagg cagtttatta ttattttgaa
    24961 tagtgtttat ttagctcagt aacaagagct gtgaactctt ttagaagatc attttcataa
    25021 aattcttgat actctttttt ctcatctgta tttttttctg aatcattaat taatgagtca
    25081 tatcgtaaaa gcgtatttag aagagctgcg agataagcaa ttggggtact cccgtcagga
    25141 aattcaattc gagatcctgt tttaagatat tttattgcgt ttgttaaata aatcttctgt
    25201 gagacagtca ggttatcttc ttcgatgtta ttgatagcat tagaaagatt agaatcaatt
    25261 atagagttat ttttttcaat agtttttttc aattgctttt caagcacatt tcgcttaatt
    25321 tcgggcatca tatgcatatc tcttaatgta taagatccgt ttaatagata ggtagtggat
    25381 acatctaata cctttgaaag ttgtttaagc ctggtgttat tgggaagatt atagccattt
    25441 tcccaattac taacggcacc tttactggcc ggtggggata atttacctcc aagttgttcc
    25501 attgagagtc ccttttcaat tctagccagt ctaattcttt gtcctgtctc cactgcttcg
    25561 gggtattttt ttctagccac attttcacca ccattcatat attttctcta ctatatcacg
    25621 atttaaatat gtgtgaaaca taaaagttca aaaaagtatg ttaaaattct tgcaaatgaa
    25681 aaggtatgat ggtatattga gtttataaga ggtatagaaa tgtattcaag agcctcttgc
    25741 atttttttaa aatctttgta tagaaatgta tgctaacgag gggggataaa tatggaatta
    25801 aaaattgaca aactcacgct tgatgatcca gcttttaaaa ttattgacga tcgagttgtt
    25861 caacaagtag agcaaactca aggaacgtta ctttcattat tagaaaaaaa gtaccagttt
    25921 ccaaaatata tgaacaaggc acaagctgct aagtatatga acgtttcata caatactatg
    25981 atgcagaaat atgttcctaa cggtttaaag ttgattatag ttgatggagt cgtaagaatt
    26041 tcacaagatg aatgtgatcg ctttatggaa gaataccaaa aataaacgca agccgtgggg
    26101 gcaaagggat taataggagg aaataataat ggaatcgaca caaaataaag caatcgaaaa
    26161 agtattatca acaattctta cagaagacac agcggcagaa ttagcaaact tggaaggcaa
    26221 agcgctagaa gaaacgtttg aatgtttgta cgaacaaatg gactatcaaa aattgctacc
    26281 acaagaacca acggtaagcg gagtattgcg tggcttgaac gatttagtac aagcagagtt
    26341 taaagaacga ctttctattg aagagtatca agaaattttg tatcagcaag ttgagttgtt
    26401 agcgagcttg ctagggcttg aactggagga cgttgaatga aaactgaaat ttggaacgga
    26461 cacattataa gatttgtcga tattaatgat gaatggtggg cagtggcgaa agatgttgcg
    26521 gaagcgttag gtcttaaaca ggttactaga gccattcact ctttgcctaa agatggggtt
    26581 actactagta aggtcatcga ttcattgggc agaacacaag atgtaaacat catcaacgaa
    26641 aaaaaatatt taccgcatgg cattcaaaag ccgtaaaaaa gaagccgagg catttcaaga
    26701 ctggattttc gacatcatca aagaactacg ccagtccaca ggacttgaag gctttcaagt
    26761 attccgcatg ctagacaaag aacaccaaaa agaagcaatg actaaattaa gccacgctat
    26821 tactgaacca aaaccagtag actacattaa agcgaatgtg atagccaata aagctgtatc
    26881 aactatttat ggtcattcca aaatggtaaa aaagaaagat atgactcctg aaatgttggt
    26941 tgatcgtgaa cctattttag atgagacagt agaattaatg actgttaaag aaaaatatgg
    27001 gttgcagttt tcagtgagtg agaaaattta taatcgttct gctgaattgc agacaactta
    27061 ggagcattaa catgatcgag aacaaagaaa aagccttatc cataacagtc ttggcggaca
    27121 ggataaaggc aacttaattg ggataaaacc cgatattctt tgttccgatt ataccatatt
    27181 aaaaaatcgg agggaaccat tatgaaacga aacttaatta taggaagcat tttaatcggc
    27241 ggaattttcg gcttattacc attaattgca aaaatagcta tctttgcagg attagcgtta
    27301 tatctcttca ctgcttatga cgagtacgac tatcaattaa gaactggagg gcataagtag
    27361 atgtacaaaa caaaactatt aaaccaactc gatagccttg aattagagga aattaatcaa
    27421 gggatcgctg aactagaaaa taatatcggg aaaacctact ttggaaattc attcaatgaa
    27481 aaactaacag ttttgtatgt actcaaaaaa catgcagaac acaaaataat ttgtcgagaa
    27541 atcaacgagc taaaaaatca gattttaacc gcatggttaa acattactga tatgcaagaa
    27601 gcaagggtga aaacatttaa tacatgggta aagtatcaga atcaattaaa aggtgctgaa
    27661 tttgttcggg atgggttgaa atacgaactt gagcagttga agctcatgga ggtgtctgaa
    27721 tgatttatac cagtattttg aaaaaggagg agaggtttat tggcaaataa ttatcaagca
    27781 gcaacggaat tacaaaagtc gggatggtca atttatccct tatctcctgg aactaaaatt
    27841 ccaataaaag gctctagtgg ttggaatgat gcaactaacg attctaatca aattaatgaa
    27901 tggtggacgg aagatgcaac tagaaatatt gggttaatgc ttgaagatca tcaaatggtg
    27961 gtagttgacc tagatcaaca ttctgaaaca cgaaacggcg ttgagaatta taaaaaactt
    28021 gcttcaatgt atgatccatt tccttctact tatacggaaa tcacaccagg taaaggaatc
    28081 catttcttct ttaaaattcc tgatggtgta attatccaac aagaaacagg tgcatttgct
    28141 gatatcttcg ggcgtgatga taaaggtaaa agtttaagcg ggattgatat tatcactaaa
    28201 ggagttccaa tagcgccaac tatgattatt tcaagaagtg ttgggagagc atatgaacct
    28261 attgaaaacg tatctctaaa taatattgca ccagctcccg attggctagt gaacctactg
    28321 ctaaaaaaac cgcaagaaat aaagactcaa taccgacccg tagcaaaaac agggacagca
    28381 aaaaaattag atatgattgt tcaaggcgct ggcgaaggta gtagaaacga tcatttaact
    28441 aagttatgcg gttggttact atggcacggt gtagataatc aaactttagc agaattgatt
    28501 tatacagcaa atgcgtacaa tgaaccaccg ctaaaagata aagaagtgaa tcaaattatt
    28561 cgatcgatga ttaaaaaaga tttgaggggg aaagctcatg gctcataagc ataaattaga
    28621 tagtatttta gactttcccg aagccagcga gcgtgaagac aatatcattg aattgaaaac
    28681 ctggatgtca cgattaagat gcaataaaga cgatcagatt aaaagtaaca gtgttgtgaa
    28741 tgcagaattg attttaacca atgatagcaa tttagctgga acaattgctt ataacgaatt
    28801 tagcggatac attcacctat taaaagattc tccttggatc aatcgtaccg ctggagaatg
    28861 ggaagacagt tttgaggatg ccttaacggc atatattgaa gaaaactaca atgtagtttt
    28921 tgatgacaac aaaatacaca aagcagtagt aaatgtggct agaaagaatg ttttcaaccc
    28981 agtaaaagaa cgtatcgaaa aagttaaatg ggatcaacag cctagactgg aaacactgtt
    29041 tattgacctt ttaggggtag acgacaacct ttacacacga gaagtaacaa aacgttggat
    29101 agtcggatca gtcgcacgta tctataaacc aggaataaag tttgagatcg ttccagtttt
    29161 ggatggaccg caagggattg gtaagagtac cgttccagca ttactttaca cggatgattt
    29221 tttcaccgat tcattagatt cgctaggtga aaagaaagat gattatatgc agcttcaagg
    29281 caatgtaatt attgaacttg gagaattatc ttcaatgaac aaaactaaaa ttgaacagat
    29341 aaaaaacttc atcagtgcca agatcgataa aattagacct ccttatggtc ggaatgtcat
    29401 agcttgggca aggcaatgtg tatttattgg aacgtcgaat gatgggcagt atttgaaaga
    29461 tgatacagga aaccgccgtt tttatccttt gccttgtaag aataagccaa agatgaatcc
    29521 gtttaaaaca aaggatgaat attttcttca agtgttagct gaagcaaagg tgctttttga
    29581 taaaggacaa cgaatctatt ttagtcccga tgaagataaa gaagtcttag aaattgcaaa
    29641 agactatcaa gaggatgcca agatggagaa tccaattaaa gaagcaatca ctaaatattt
    29701 ggaaatggaa atcccgtata aatgggaaga agctccagcg tgggcgagac gttcttatta
    29761 tcagaactat cctgacagta aatgtaatga gaaaaattta cagcatttct ataatggagc
    29821 tactatgcaa caagagtttt atttagttga tagcgtactc accgctgata ttttggaagc
    29881 tgtttttgat aaacaagcaa aggatttatt gaatggtcga tcagatgcag aaactaaaaa
    29941 gattgccttg attattagca gtattcctgg ttgggaacgg aagcaactgc caaaaagaaa
    30001 taaacgacga ggattttaca ataagtcaaa tgcctatgaa aataaaaatc gagctggaaa
    30061 gtacaaaaag taacgggtag taacgggtca gtaacaccta gcccgttact agtttaccct
    30121 tactgcccca agggatacag caatttggta acggggtaac accttatttc cttaaaactt
    30181 ttgaaatagt tagctaatat gctggggaat aatgttactg gattttaccc gttacctgtt
    30241 accaaaacgc tagaaccctt gtccccgttg ggattgagtg gtaacgggta cctgttacgt
    30301 aggtgttacc acgtgttacg aatcaaggag gaaagtaaat gctcaatatt atttcaacaa
    30361 acaaagcacc gaactttcaa tatacagatg aaatggatcg gtttctaatg aacacgctgg
    30421 catttagtgt gggattggta acagaagatt attccacatt tgatccagaa gtattaaaga
    30481 taatggaaga agaacctgat tggctgcagg agtcagtagc gtggtgtcaa tcgctagtag
    30541 tgggatcatt ggtagatagt ggcaactatg atgatacagg tgagctaatg gatgaattta
    30601 attgtctgct taacctatat gatcgagcga gacaacgaga gcttacatcg aatgaggata
    30661 atttgttttt aaacatccat gataagttct tggctttgct actaacagat gatgaattga
    30721 taactaattt attggaggta gaataattat gcatatggaa agtattagat acttcgatgg
    30781 tgagaaacac attgagtatt caaacactca agaagatgta gactttatta gttttgagga
    30841 agataaaatc actaaagtta attttaaaga cggaacgtgc ttgaagatag tttcgcctta
    30901 tattgaatac aaatcaaaat gggaacaaga tgattctgac gaacgagatc gctttggttg
    30961 gtaatctatg gcagttaaaa aacaatgtaa tcatgctgga tgtaaaacat tgattgatta
    31021 taggcagaag tattgtggga agcataaagc aaagcaaacg gcaattaagc gtgaagaaag
    31081 aaagcaaagc gaaggtaaat attttcaatt ctatcaaagt agaacatggc gaaaggcttc
    31141 atacttgtat cggttaaatc atccagtatg tgaggattgc ctagaagaag gcttgataag
    31201 gaaagctgat gttgtagatc acaaaattga actaaaagat gattggaata gacggttaga
    31261 tgaaactaat tttcgttctt tatgccatgc acaccataat tccaagacag ccaatgaaag
    31321 acaaagacga gaaaagagta ccctttgagt taagggggca tagtgtgaaa cttactgaca
    31381 atcgatgcct actcattttg gtacaaataa ccgttgtgaa aaggcataag ggtaattaca
    31441 aataacgatt acacttgtaa tcacaagtga aaaagtatat aatgaaagta ggagatttat
    31501 tgaaaaatac aattgttatt actgataaaa cagggaaaca gcgcaccatt gagttacctc
    31561 attttggtca agtgaccatt caaatgcaga acggaaaaat catttatata gataaattag
    31621 ataaagaaaa attctgatcg aaaaacgaag gaatgtgagc ttaattgctt acgttccttt
    31681 ttcttttgtc tgaaaggagg acaacatggg acaccctaaa atattacacg atacaaaagg
    31741 caatatatca agtgaagaaa aagcaatccg tgtggacgct agagaagaat tgttcaagca
    31801 gcaaccatta ataaatatca cgccccctga ctggatggca gcgagcgcta gaagtgaatg
    31861 gaatcgtatc gtaccaacat taaaaaaaga ttatccattg agtgaagcag attatggttc
    31921 attggtagcc tattgtttag cctttgctcg aatgaaaaca gccgaagccg agataagaaa
    31981 atcaggtacg tttatcacat gcgaaaacgg agtaaagaaa gccaatccag cggttcgggt
    32041 tcaatcacaa gctatgagtg atttgaaaaa acaagccacc tcactaggta tgaccttaga
    32101 atcacgatca aaactagctt tgaacaaggt taaaaatgat gaacccgaag acccgttcaa
    32161 agagttgatg aattcatgaa tgattacatt gagaaagtcc tatcaggtaa gctgattgca
    32221 cccaagaaaa ttatccaagc gtgtgagcgt catataagcg atttggagcg ttctaagtca
    32281 gatagcttcc cttatgtgtt cgatgaagaa caagccacca aagcgattaa attcattgag
    32341 ttgctaccat ctacggacgg taaagcaatc aagatgttag gatttcaaaa attcattcta
    32401 ggcagtcttt atggctggcg tactaaagaa gggaattaca ggcgattcaa tcgagcgttt
    32461 accagtatga gccgtaagaa tgggaaaacg tatatcgcaa gtggcatggc tgccaatgcg
    32521 ttgattatgg aacaagaacc agcagaagca aggcaagtat tgtttgtaag taacgctttg
    32581 aaacaagcta aattgggcta tgatatgctg tctaattcac ttagaaacgt ggtcaagtct
    32641 agtaagtttt taagaccaca actgaaaatt atgaactcta agattcagca cttgccctct
    32701 aattcgttcg ctatggcact ggctagtgaa accagcacgc tagatgggtt tgcaccaaca
    32761 accgcaattc ttgacgagtg gcacgaagca aaaactcgta aaacgtacaa cgtcattaag
    32821 tcaggaatga cccaacaaaa gaacggctta ttgtgtgtta ttagtaccgc tgggcttgat
    32881 ttaaacgttc ctatgtacga ggaatactta ttgttagatc gtgtgctaaa aggcgaagaa
    32941 caagccgaca ggtactttat agcgatatgg gaattggacg atcccgaaga aattcacgat
    33001 caagagaaat ggatcaaagc caatccgatt tttgaaagtg aagaaatcaa aaaagtaatg
    33061 attccaacca ttcaagatga tgtgaacctt gctttgaaac aaaataacct taattctgta
    33121 ttggtgaaaa acttcaattt atggagacaa gcgagtgagg acagctatat gattgctgaa
    33181 gactggcagg caaccgaagt agaaccacaa gatattacag gcaagcccgt ttatatcgga
    33241 gtggatttat ctaaaacaga tgatttaact agcgtttcat ggatcgtacc gctagataac
    33301 ggcgaacttt attgtgattc acatagcttt gtagccacca aatatgggct tcaagacaaa
    33361 gaaaagcgcg acggtttgcc ttatcgagaa ctagaaaaag ctggtgagtg ttccattact
    33421 caattagaaa gtggaatcgt ggactatgac caagtatttc agtttattca agatttgatt
    33481 caagaaaatg atttggaatg tatggggatt tgttatgacc cgtataacgc taattcgctt
    33541 atcagtaaag ctgaaaaagc caactaccca atgttagaag taagacaagg aacgattact
    33601 ctaaacgttc cgacccgaac ttttagagaa caagtttatg aaggcaacgt tattcacaat
    33661 aaaaatacga ttctcaccca tgcagtgaac aacgctattt taaaaacgga taacaacggc
    33721 attcagatta ataaatcaaa gaacagtaac aaaattgatc caatagctgc attaatcaat
    33781 gcctatgtgt ttgcaatgga ttacttcacc acaacggaag gagcgaaagc agacaatgaa
    33841 ttttatacaa gtgaagaatt ttctttctaa ttacattcat accgttcttt tacttcttgg
    33901 attggtgtgt gtgttggttg ctatcacctt actaacaaat gtctactatg gcttgttagc
    33961 gctgggcata gtgcttattg gggtagcggt catgctaaat acagaacaga agggaggtta
    34021 aaagatggca ttttttaaag cgagacaaaa tacaacggga gatcctttcc tacaacatgt
    34081 ggtatcaatc caatcggatg attacaccac cagctttaca agcgttcgtg cattaagaaa
    34141 tagtgatgtg tttgcagccg ttcgaattat tgctagtgat attgcttcaa gtccgattca
    34201 attggtcaaa aacaatatgc cacaagctga taatgaactg gtgaagttac taaacgagaa
    34261 acctaattca gaaatggatg gttggcattt aaagtttgct ttggcggtca atatgttgct
    34321 aaacggtaat agctttgcag agattaaacg caacggtgaa aaggtagaag aaattcactt
    34381 attacctaac tcaagtgtaa cggttactca attagataat ggtacgttgt cttatcagat
    34441 tggtgataaa aaaagacgtg tgaagtctag cgatattttg cacttcaaat atttcaccca
    34501 agatggttta acaggattac caccacttta tgcgttacgt gatgaactga aaattcaaca
    34561 agctggcaac cgcacattgc acaatttctt tactcgtggt gtcagtgggt caggtatttt
    34621 gaaggttcat aagtcagatt tagacggatc agcaaagaac gcaattcgtg aaaagtttga
    34681 agaagccaac ggttcaagta gtggagataa tgctcttaga acgatcattc ttgatgaaac
    34741 aatggactac aaaacattag aaataaacac cgatgttttg aagcttatca attccaatga
    34801 ttggaacaca aaacaaattg ccaaggcgtt tggtgtaccg attgagcgtt taggcgttga
    34861 aaatgaacat tctagcaccg ttcaaagtaa tctccagtat atccaaagta cgctgattca
    34921 ttactttaat gtgtttgtga gtgaattaga tacgaaactt gaaaccaata tccgctttaa
    34981 ttccgatcag ttactagaga ctgatcctga aacaaaagta aaaaatgtat tggatcaggt
    35041 caaagggtca cttctcacga ttaatgaggg gcggtcgaaa atggggctac cccccatgga
    35101 tggtggcgat cgtttactag caagtttgaa ctttacgtat ttagatacgt tggagaaata
    35161 tcaattaaaa gaacaggaag gagttacacc agttgaataa tgaagaagaa aaggaaaaac
    35221 ggctgacaga agaagctgag ctaacagccg attctcccaa agtagagaaa gaaaatgaaa
    35281 aacaaccaac agacggcaaa actatttcag gctatgcgtt gaaattcggg caaccgtcaa
    35341 aagatttagg cggctttgta gaagtgatta cacccgaagc attaaaagag gtggatttat
    35401 caaatgtgtt cttattatat ggacacgatt attcaaagcc actagctagt gtcaaagcag
    35461 gtacgttgaa attgaatgtc gatgatacag gattacattt tgaagcagag ctaaccgaca
    35521 cgacctattc aaatgatgtg tatgagaata tctcaaaagg ggttgttgat tctatgtcct
    35581 ttgggttcgt gcttggatta gattcattcg acaaaaaaga agatggcaca attgaacgat
    35641 caatagataa aatcaaagca cttaatgaaa ttagtgtcgt gaccgttccc gcttatgatt
    35701 catcaaatgt ccaagtaaat aagcgttctt acgaatcgtt tatgagtaac aaccaagcaa
    35761 aacaaacaaa caatagctta gaatccactt ctaaagcaca aaaggagagt aataacatgg
    35821 aaaaaacttt aatcgataac gaaaaaacag aattgcgtgg gtatgaagaa tatatccgtt
    35881 cacaaggcga agtgcgtgat ggagtcacta ctgtaaatgc agcggcagtt gttcccgaag
    35941 aagtaatcgg tgaagtcttt gatttgaaac gttcaaatta taacttagct caatatgcaa
    36001 cagtaaaaac agtatcaaat ggacaaggta aatatccagt agcaactaac caacaagcag
    36061 tgttagcaac aaaagctgaa cttgctgaaa ttggtgatat tgacgctgaa atgtttactt
    36121 cagttgatta taaagtagaa actcgtgctg gtaagattgc cttatcaaat gaggttgtgg
    36181 aagattcagc agtgaatatt gtacaagagg tcaaagatca gttagcgaaa ttggtagaaa
    36241 acactgacaa taagcatatc atggatttat taaaaacatt cactaagaaa acggctgcta
    36301 cgttggacga tttgaaacaa ctatacaatg tggcattaga cccagcatta aataaaatgg
    36361 taattctaaa ccaaagcgga tattaccacc tagatacatt gaaagattca gatggacgtt
    36421 acattttaca acccgatgtg acagcaccta gtggtaaatc attattcggt atgccagtag
    36481 tattgattgc agatacattg tttgccaatc ctaaagctgg aacattccct atgattatgg
    36541 gggatattgc acaatctatc tttgttgctc gtagaaatca agtaacgact caatgggaaa
    36601 aattcgatta ctactcacaa ggacttgcag tgatcgttcg caacgattat aagaaaattg
    36661 atcaaaatgc ttcagtgtat attgagttta cgccagttgt aacaccaaaa gtatagaaaa
    36721 atattgggcg gcggtttatc ccactgcctt tttttcatta ggagagataa aatggtaatt
    36781 ttggatagca taaaaaaaag tatgcggatc gatcacacta ttgatgatga ctttattcaa
    36841 caattgattg atacagcagg cgaatatata aaaagtgcta ttgatagtag cgcaacggat
    36901 aaagatatgg ataattatca gcaatttgat ttggcggtgt cattacttac tcaacattgg
    36961 tatttgaacc gtcaagaagc cagcagcgaa cggataccag taacggtaca agcattagta
    37021 caacaaatga gaggtgctta ttatgccaat cattaagaat gtaaatgaat tgactgagag
    37081 agtcaagttt aaaaagacaa aacgggtaaa agatgaagat gggcagatgg tggatagtga
    37141 agaaactgta tttgaatgtt gggctaatgt gcgttcacaa atgctaaaag acgtgcttgc
    37201 tagtgtaggt accattcttg aaggaacgtt gacgtttatt attcgatacg atcaagatta
    37261 tgaactaaca aacgatatga aagtagcttg gaaaaacaag agttataaaa ttatttcaat
    37321 caatgaagga acggcgttta aagattatac aacgatcata gctaaacaga tttcttagaa
    37381 tgattacact tgtaaacgtt gtgggtattt agtataatta aggtagtaaa tgaagggtta
    37441 gctgcctgaa ttttgcaaga cttagctagt cgaaatctat tgttggactg aaaattgtag
    37501 gtgtggttgc aaacatgctg gactaagaaa gatgatagat gatgaacgtt caattttaat
    37561 acaggcatgc taggactgat tggatggttt tggcaagctg attgtgtaga aatcaaagaa
    37621 cacgtctgtt gtggacgtgt tttttttgtt ataattcaaa agaggtgatt taatggacaa
    37681 agcagagttt acaaattttt attggaagta ttatctacat ttagaaaatg agtttattaa
    37741 taccacagat tttgtgatgc tcgatgagaa taatttcaaa acattctcta ttgaatatca
    37801 aaaactacta ctagcgattg gttcggagtg tgagattatt ttcaaagaac tttgcggatt
    37861 tgatagcagt agcaataaaa gcataactga ttttaaagct gttattaaat ctagcgaatt
    37921 aatgtgtttg gataatggtg ttagggttat gaattcttta acattgactt cactaaaacc
    37981 ttttggagat gggtggcctg aagatacacc agattggtgg aaaatatata acagagtaaa
    38041 acatggtcgt tcttcaaatt ataaggaagc aaatttggaa aatgtgttgt atgctttagc
    38101 aagtctatat ttattagagg aatatttgca taagaaagta atactagaag gtgaagtaga
    38161 ctttataagt ccagagtcaa atctatttac gttatcttgg gaaagaaaac attcgtcgat
    38221 gcaaaaagtg acgtttgaaa aagtggattc aaattatgta gctccactca ttgattttaa
    38281 agaacaagaa gaaatttaaa aacccccacg gcttattgaa gtttttgtat gccatattgt
    38341 atgccatttg gtttaaatcg cttgaaaaat agtgatagat aactctaatt catttagtca
    38401 taaacgctgt tttgataacc tttatagttc tttgatatgg gtttctcatt catggcggaa
    38461 gaagaagaat agaacaaaag aatgaattaa taagatataa aaaaagggat tgtgacaaac
    38521 gtcttgtcac aatccctttt tccaaataaa cgatgttcaa tcagctgccc ctcggcattc
    38581 gctcgaaaaa cggcgaaata tgaagaacta ttttgccatt tgtttctcta ataatcaggt
    38641 caaaacgttg acttcacagt ctcttcctta aacggtgttc tcctgccaac tcctcggtca
    38701 caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgttcc ttgtgactcg
    38761 gagctgaacg gcaggctcac gacctctttc ttaaacggtg ttcaaaagct tgcatctgcg
    38821 gtaataagtt caaccaaacg aaaattatga ggagctattt ttgtttggct gttcttatta
    38881 cttgatgcag gacagctttt tcacaacctc ttttcgttta gcactaaatt tccttaaaaa
    38941 gtagaaaagc tttcgaagcc tcgtttgttt cttttactgt ataataaaag aaaagagtaa
    39001 caatcgctaa aagatataaa caagagaaag gaaatacaag atgattggtc tgatttattt
    39061 tgtggtgatc gttctggcga acacagttgg cgctatttcc gggatgggtg gaggcgtgtt
    39121 gatcaaaccg attcttgatc ttatcggcgc ccattctgtt gcagggattt ctttttactc
    39181 aacagttgcg gtattcacga tgtccattgt ttcgactgtg cgacaagtat caagcgggaa
    39241 atcactgaat tggcagattg tcggttgggt atctagtgga gctgttgttg gaggagtagt
    39301 cggcaacatt gtattcgaag tttttttaca gttatttgaa aacgagaagc atgtgcagct
    39361 gatccaaatt tttttgacgg tattgacctt ggtttttgcg ttcttctaca ccaagcatca
    39421 tcagccaaaa tttcatttga cgtcatggac ttggtacctt atatgtgggg ggggttctgg
    39481 gctttttagc tagtttttta ggtattggtg gtggaccagt caatgtttct ttgttgatgc
    39541 tgatgtttgc attgccaata aaagaagcaa cactatattc tctatctacg attttctttt
    39601 ctcaactggc aaaattggta acgattgctt taacaagtag ctttatgcgc tttgatttat
    39661 ctatgttgtt ttatgttatc cccgctgctg ttattggagg tttgtgggga gcaagattta
    39721 gcagggtttt gtctccaaaa aaagtgacct ttatttttca agtaattgtc ctcgtggttc
    39781 ttttggtcaa ttgttacaat gcctttgtca tcttacaaac attaggagaa tctagactaa
    39841 cgcaagtcaa aatcactgaa acatattgtt ctaaatgaaa aaaagagtat actatgataa
    39901 aaaaattcat tttaaaaaat cagaaattgg ctggtgattt ggatgttacg ggaaatgcgt
    39961 ttagaagatg cagaagcact acgtgaaatc aatgcggatc aattagggta tgatatacca
    40021 ttaaccgtta cggtgagaca aatgaagaaa ctgctgaagg agccagagaa aaattttttc
    40081 ttggtttatg aagatcctac ttctaaaaaa gcagctggct atgtacaagc ggagtcctat
    40141 gaatcgattt ttagcgatcc aatgttcaat attatggcac ttgctgtgtc taaatatgct
    40201 gaaaaaagag gcatcggaaa ggctttgatg actggcgtag aagaagaagc gaaaaaacga
    40261 ggaataacga caataaggct taattctgca gaatacagag tagaagcaca ccaattttat
    40321 gaacacatcg gttatcattc cgataaaatg caaaaaagat ttttgaaaat cctgtaaatc
    40381 tttatcaaaa aaatacccga tcaccaagcc gatacttgat gatcaggtat ttttgttttt
    40441 ttgactaaga aagtaattgc atcaagaaac cagagatgat caaaacgatg gcaccaccaa
    40501 gtcgattgcc catttgagca aaagcaataa gttccatccg attagctgca gataagacgg
    40561 ctacatttcc tgttccgccc atcgagttgt tgcataagcc agcagtaata gttgattcaa
    40621 ttggatataa accaaacaaa cgaccgacaa atcctgaaac aactgaaatc gcgataacac
    40681 ttgtcagaca taagacaaca aattgccatg tcaaagcaga agctaataca ttcagattca
    40741 gcaacgcaat accgataccg gcaagaacag ctgctgtcaa gtttttgaca atcaagttat
    40801 tgaacatgat agctgcatct tcataatact ctggtaatag gttggcgatt ttacagatga
    40861 caacgataat aatcataaat gcgtaagcat ggactttagg aacaaaatag ttaccaattt
    40921 gtcctaagat gaagaaagtc aatgatacca tcaaaccaac acctaactgt ggaatggaag
    40981 gtttcacagt tcgaggtttt ccatcgccaa gatcgccttt ttccattaat ttaccattgc
    41041 cgttgaattt tggcattgat tgtccagcac gagcaactaa tgctgcaccg atgatagcta
    41101 agacatttgt cattgctgtc gcaggaatca ggcgggaaat gatagaagaa gcatccgtac
    41161 ctaaaacatt tgcataaatc gttgataaag gaacggctcc ggctccaaca ccaccagcca
    41221 tcgcaggtaa tgagatgtat aaaacggaat ttgcaaaacc atttccgata agcatgccca
    41281 cgagtcctac ggcaaaaaag gagacgacca ttgaaataag tgcaacggga ataaaacgaa
    41341 cagaagcttt catcaataga ttacggttca taccaaggat gctaccagta atcaaggcag
    41401 caatataaaa gtcccaaaag cccatattgc tgacaaagtt ttctgtggat ttcactacat
    41461 attcaggtaa gatcccaaat gtagcaatcg cagcagaagc gaagatcgca aaaacggaac
    41521 caccacccaa ataacttttg acgataggga tttttgtccc gataaaatga aataaattac
    41581 cgaaaataac caaaactgcg attggtccaa tcatattcgt cggtaatttc cctaaagcca
    41641 ttgcaatgat caggacaagg accatgatca aataaagtgg caaacttacg ccggtgattt
    41701 ttgtggacca aaaaccttct tttttctttt cctcaactgc agttgcagtt gttgtttttt
    41761 gtgccatttt tacagatcct ttcttattct gtatcatatg aaaaaagcca aagaaaagcg
    41821 acaaaagagc aggagttggc tggccgctgt tttgccgcag tcgctttttt ctttttagac
    41881 gtcatacaaa gtttctgtat gttcttttac tataacgatc ttaatcttct aaagatttat
    41941 attcaggaac ccatttagca gattcaactg cttctttgat atccgtgatt tcttcacgat
    42001 tcaagttttg tgcgacaact gattttgcga cagtttcagc aatcgtttga gaaaattcag
    42061 tgatttttgc tactggaggc aagatagctg ctcctggttt tgttacatca acgattccgc
    42121 ctaatgctcg actagcttga gaaatgattt cagcatttac tcgtgtagct gttgaagcaa
    42181 tcaaaccaag tcctaaacct gggtacatca aagcattatt tgcttgaccg atttggtaag
    42241 ttacaccatt atattcaaca tcagcagcag ggatacctgt tccaactaat gcttttccgt
    42301 ccgtccattc tattaaatct tttgcttttg cttctgccaa ttttgttgga ttagacaaag
    42361 gcatgatgat cggacgtggt gtatgagcag ccatttcttt cacgatttct tcagtgaatg
    42421 ctccaggttg ggtagaagta ccaatcatga ttgtcggatg gatcgctttt acaactgctt
    42481 ctaagtttgt caattcatca gcattagtga aatctgagcg tttgcgagcg aatgggattt
    42541 gaccaggagt caatccttct gtatcttcaa acaataaacc ttgtttatct acttgataga
    42601 aatgttggcg tgcttcttct tcaggtacac cttgacggat catctcatct aaaaggatgt
    42661 ttgcgatacc cacaccagca gtaccagcac caaatgtcaa aattatttga tctttcaatt
    42721 gttcaccgga tatattcaat ccgcctaata cacctgctag gacaacaatt cctgtacctt
    42781 ggatatcgtc gttgaaagta gtgatttgat cttcgtattt ttctaagatc gctgcagcat
    42841 ttcctcgtcc aaaatcttcc caatgcaata acaattctgg gaataattcg gtagctgatt
    42901 gaacgaattg atcgatgaat tcatagtaag tttcgccttc tacacgagca tgacgattgc
    42961 ctaagtataa gggattattc aataattctt cattattagt accagcgtcg atcgatacag
    43021 gcaaaacttg tgctggatta ataccagcag cagcagtata gaccatcaat ttgccgatag
    43081 cgatgtcaac accgttcaca ccccagtcgc ccatacctaa gattccttct gcatcagtca
    43141 caacaatcaa acgaatgtct cgtccatctg ccgcgttctt caaacttgct ttgatgtttt
    43201 ctggttcgtc gattgaaaga aatgcagcat cttgcggttt caagaaaatt tcgttgtatt
    43261 gttcgattga atccgcaacg acaggatcat aaacgacagg catgaattca actaaatgtt
    43321 cgcccatcaa tttataaaac aatgtccgat tcgtattgaa tagattcatt aagaagatac
    43381 gtttttccaa atctgtttgt ttgcttaagt attgaccata agcttgaaca gattgttgtt
    43441 ccagtgtttg gacagtactt ggaagcatac cagtgattcc atattttgcc cgttcttctt
    43501 ttgtaaaagc tgttccttta tttagaaaag gattatttaa taagtttaac cctgtaagca
    43561 tgaatgattc ctcctaagtt ttaatacaaa cgaagtatag aagtcagatg atgatatagt
    43621 caaaaagagc aggtatgata aattttattt atatgtaata tagccaaaag aaatcaaaat
    43681 agcagtattt gttcttttta tatcgacaat ccttgcttga atactatgcg tatctcttat
    43741 atgctagaga aagaaaagta gaagaataag ggatagatgg aatggataaa taggatttct
    43801 gaggaaaaaa gcacatgata gcgttgatag gagtcaccta tgtcaggtga taaaaataca
    43861 taagtagaaa attaagagga ggaagagaaa atgaaagaag ttcaattgat tagaaaaagc
    43921 gaactatcag aaggcggttg caatgcatgt ggcgtagtag aagcgacaag ctatacatta
    43981 aaattagatt ccaatcaagc aataatctct gaactgacag taggcggtct tgtggacagt
    44041 ctggcgcttg ctgaaggttt tactggagaa gatatttatg agatgttttc agaagtccgt
    44101 cagttaaaaa aaggagaaaa atgcatagag gttcatcatg agagcccgaa tgtgaggttc
    44161 aaacgtggtg ataatgagat gatcttcaag aatcatgtgt ctgatcacac tgaactatat
    44221 gaaatcgtga atcaaatact gtctgggatt tttgaattag gaccttatga gttcaaggaa
    44281 gaaaacggta atccaaaatt aaatgaagaa tggcaagaaa cgattgaaac acaaagaaac
    44341 aatccacact tatttcaata gtcttccaca gtatttcgta aaaatgttga tgaaaaagaa
    44401 acaccgtgaa tcagaggtcg tgaaaaacct gccctgcatc aagtaataag aacagccaaa
    44461 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagttttt
    44521 gaacaccgtt taaggaagag actgtgaagt caacgttctg acctgagtat tagagagaca
    44581 aatggcaaaa tagttcctca tattttgcca ttttttgagc taataccgag ggtcagctga
    44641 ttgaacaccg ttttatcgag aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa
    44701 attttgtgaa aatggctttc cacatttttg cataaatttg acttaatacc gagaaaatgg
    44761 cttttgaaca ccgtttattc aaaaaaaggg actttgacac tacttttgtc aaagtcccat
    44821 cggtttttta tctattttta ttacaatcat ttcttccaga gtagtgacat ctggaatggc
    44881 tgaaatgtaa acagattatt tgcctgtatc aatgtcgaag agtaatataa aaagtgtaaa
    44941 cgctatcata ttgttcatca ttcaaatttt atagagagaa ggcattgaga tggaaaaaac
    45001 atatcaagca ggtacaaatg aaggaatagt agattttatc aatatggaag atttagaaat
    45061 cgcggcttca caagtgattc cagctggtgg ttatggttac atcagcagtg gtgctggcga
    45121 cttatttact tatcaagaaa atgaacgggc attcaatcat cgtttgatta ttcctcatgt
    45181 attaagagat gtagaattac ccgatacaac tacacatttt gacgaagaaa tgctgactgc
    45241 accaatcatc atggcaccag ttgctgctca tggattagct catgtgaagg cggagaaagc
    45301 ttctgcaaaa ggtgtagcag attttgggac gatctataca gcaagttctt acgcttcttg
    45361 tactttagaa gagataagag aagcaggcgg ggagaaagca ccgcaatggt ttcaatttta
    45421 tatgagcaaa gataatggaa tcaatttgga tattcttgaa gtggccaagc gtaatggagc
    45481 aaaagcaatc gtattgacag cggatgcaac agttggcgga aaccgtgaaa cagaccgcag
    45541 aaacgggttt accttcccgc tgccaatgcc gatcgttcag gcttatcaat caggagtagg
    45601 ccaaacgatg gatgctgtat ataaatcttc caaacaaaaa cttagtccaa aagatgtaga
    45661 atttatcgct gctcattctg atttgccagt ctacgtgaaa ggtgttcaat cagaagaaga
    45721 tgtctatcga tcattagaat ctggtgctgg tggaatttgg gtatctaacc atggtggccg
    45781 tcaattagat ggggggcctg ctgcgtttga ttcgttgcaa tatgtggcag aagcagtaga
    45841 taaacgtgta ccaatcgtgt ttgacagcgg tgtacgacgt ggacaacatg ttttcaaagc
    45901 aatcgcatca ggtgccgatc ttgttgctat tggcagaccc gttatttatg gattgtcttt
    45961 aggaggaagt acaggagtcc atcaagtgtt tgatttcttt aaaacagaat tggaaatggt
    46021 catgcaatta gctggaacac aaacagtaga agatatcaaa aaaataaaat tgcgtgagaa
    46081 tcgttttatt taagaaataa atgacgaaaa aaataggagt gcggcagatg atgccgtact
    46141 cctattttta tagttcaagg atgtcactaa tagaataatt tgaaacgaaa aaataaaatt
    46201 gaaatatttt ttcatactta ataaaaaagg aagaatattc atgtaaacga ttttattgtt
    46261 tttttctctt ttatacaaaa agggtcataa tttcaagtaa gcggatgaat tcatgaggat
    46321 tttttgcata ctgtctctac tatacttagg tcactaagaa aaaggagtga tttattatga
    46381 aaaagaacat gattgggaca ttattacttt caacacttct tctaggcggc ttggcaacac
    46441 cagcttttgc tgagggtcaa gcgacttcaa aaggagatat tacctttact gaaccaacaa
    46501 ataccgttga gcctcttaac cctactgatc cttcaaaacc agtagagcca gcagatccgg
    46561 aaaatccagc aactggccag acaggttctt taacattgga tgtagttcct gaattgccat
    46621 ttggtacaca cgagattgaa agcggaacaa aaacgtatca agtagacgct tcaaaaaacg
    46681 atacacctta tctccaagta tcagatcgtc gaggtgtagg tgcagatggc caagcacaag
    46741 ggtggaacgt gacagtttcg gtttcagatt ttgtaaacgg ttcacaagtt ttgcaaggcg
    46801 ctgaattaga ttttggaaca tccactgtta aaagtacgtc agataacgaa tccactggac
    46861 caactagtca aactgtaact gggttgagta aagcaagtgc agcgacacca atatttacag
    46921 cagcaaaaga tcaaggtctg ggaacatggc tttctgttta tgatccagca aatatcacac
    46981 tgaaagtgcc gaaagcagca gctggaacat tcacggctga tttgacttgg aacctcgtgg
    47041 ctggcccagt agcatagagt atgaaataaa ttcaacaaac tgcggtgaaa atactatagg
    47101 tgaaggattt tttaccgcag ttgttgttga aaggatgtgg tggacaatga atcaaagtaa
    47161 acgactgttt gtttcgtttt tttctatttt aagtctattt ttcatttttc cgtcaatcag
    47221 ccaagcaaag gattcagcag gagactttgg aatcaaaccg gtatttccag agaaccaaat
    47281 agataaagca atcggttatt ttgatttatt agtggcaccg gaacaaaatc agacactaga
    47341 agtaattatc agcaattctt cagatgaaga acgaacattt gaggtctctg tcaatccggc
    47401 ggtaacgagt gatggtggaa cgatcgatta tagtcaaaaa aacccaacgt tagatgagac
    47461 attgccgttt gatgtccgtg acgttttgct cattgcgaaa aaggaaatca atgtatcagc
    47521 acatgcagaa acaactatac ctatagaagt gaaaatccca gcaaaaagct ttaagggacg
    47581 tgtattagcg ggtattcatg tttcacctaa agaagaagct gaaacagaaa atgctacaga
    47641 aggagcacaa atcaaaaatc gtattgctta taatctcgca gtcgttttac aagaaagtca
    47701 agagacgata gaaccggatc tgaaactttt atcaggagat ttggacgagg tcaacgcaaa
    47761 gccaactgtt cagcttcgtt tccaaaatcc tcagcctagg attatcagca atttgatatt
    47821 tacgagcaag atcttttatg aaaatcagct ttatattgaa aatacgtcta atgaattttt
    47881 agttgcacca aattcgaatt ttcacttgaa tcttgattta gctggtgata aagcaaaagc
    47941 cggcgattac cgcgcagaaa tcatagcaaa atcaggcgac agtaatgaat ggcgatttac
    48001 acaaaacttt acgatcaaaa aagaaaaagc acaaaaagta aatgaaaatt ccgtttttgc
    48061 agtacaagaa caatctttcc catggcttta tgcaggattg gttgctgcag ttctagtgtt
    48121 atggttgatt ttgctgtttt tgtgggtcca caagaaaaag aaaaaaggag aaacaacatg
    48181 atgaggtgat ttcgttgaaa acatcgatct tattgggagg gattattgca agtttgctgt
    48241 tcttcagctg gcaagagcca gtccaagcag ataccaaaag ttcaggtacg attacatttt
    48301 atgatctaag tccagttccg catccaagtg gttcagtcaa tggcgcatca aaacagcaga
    48361 cacagctacc gatttataaa gaatctaatc aacatggcgt attcccggag acgaatgaag
    48421 aaaagatccg ttttctttat ttgatcattg gtatagaatt atggcttctt gcgtggttgt
    48481 tgttatggag aagaaaggaa aaggaggatg aacatgttgc tacaaaataa attttcattt
    48541 tttcgctcct tcttttttac ttcgcttttt gttttaagta tatttatctt ttcaccttcg
    48601 gcagaagctg ttggagcaac gcctcctgcc atttcttatg atccatacac gtatgaaaat
    48661 atcgtacctc caaatggtgg acgtagtgca gtcaatggcg ggaagcttgt ccaactaact
    48721 ggggctaacc aattgcctaa attgactgct acttatttga acgatggttc aaatgatcct
    48781 tggaaaggtg gcacggtccg ttttcattgg gaaagccatg tgaaactaac aaatgtactt
    48841 gatttggtga atatcactgc acaaggattt gcatttgtgg cgatcatcaa tttgccggaa
    48901 catgtatcaa gcagtgatgt attaaaagca attgactgga acaacgccta cttgtatatt
    48961 gccggtgccc ccatcaaggt tactagcggt agtttattat cagcaggaag ccataccttg
    49021 cgttttggga tggggtcgtg ggatacaaat actctattgg cagatattat tgctattatt
    49081 acgcgaggcg aattcaacgt ggacgatatc ccgcttcaat ttgatgttac agctaatgtc
    49141 gcgaagatga cagaaaacgg agataaatat gatacgagtc caaacaaaat tttgaccaag
    49201 ggtaaattac aaccttctat gtctaaaaag gccgtttttt ctgttgattt ttatgattcg
    49261 aataatatta tccaagatcg tatgttcaat tcgattttag ctccagctgc aggtagattt
    49321 tatcctaaat taactggcgg aaacttgaat ccaggagcat atgcattttc tgcaagtgca
    49381 gagataaata cgtggaatag ttacatctca acaagtgatt caacaggaaa atataataca
    49441 tttgctgata ccacagaata cgtggatgga aataaccaga accttccagg ctctaccaat
    49501 agtcggacat tgacgatggg gctgactgaa ggacgttttg atcagttgcc atttagtcgc
    49561 tttgatcgcg tagtcaacta ttttacagga aaaaacgtta cacaaggagc aatactgact
    49621 catactccag caagcacaag tggattgaat cagacagcca atatcgtgta cagtgggaaa
    49681 gatgcgcaag gcggattgct ttctcctgtt gctttaaagg tattggagca gtacaagcta
    49741 gacggaacgg ttttaccaac aaacttaaca gccgatagtc agtggggaaa gctcctttct
    49801 tctgcatcgt accaagtcac ggccaattgg tcagcaactg aactaaaaaa gggttcgatc
    49861 cattacaggc tttattcgaa aggaacccaa aaattagcga caggatggac agatcaactt
    49921 ttccaaacga tccaatcaaa taacgggggt gtaaatacag ctacttctac actacctgca
    49981 ttggcttcag ggcaatatta ttttgattat cgcttggtgg acgatgtact tactaacatg
    50041 tatccgtctt ttgcgtacaa atggcagtct gaacaagctg gaatcaccac tcttcctcaa
    50101 ataacagtgg ctaattttcc atctgtgagt gcagatagtc agctaaaaaa ccttagcaga
    50161 caacctgaga caggagatcc cctgtctgct ctatcaggag atacgattca agaaaatgtg
    50221 acattcacgt taacaaaatc tggggatgcg ataaccgaca aaaaaatcac gatctcactt
    50281 cctgaaaata ctacttatat caatggttcc ttaaaattaa atggtacaac tatcgcagat
    50341 accggcattc aacaaggagt ttctgtccca gcagatctat tgagcaaaat aggagatacc
    50401 attcatctta cttacaacta tcaattaaat actgtagata cttctgtaca atccgtcagc
    50461 atactgacaa aggcagctgt actttccagc aatattacat tagcagatgg tgctaagctt
    50521 cctaacccag ttgtccaaac atctgctaaa acaattcttg tccccaaaca agaattaaca
    50581 ttagttaatg taccagatga ttttacattt ggtaacgatc tccccaaacc gttgaagaca
    50641 agttattatg aagcaaaagg cgatttttct tttgatgtgc gggatactcg gcttccaagc
    50701 acgtctcctt ggcaactgac agggacattg acttctttat ttaagaatga tcaaggtcaa
    50761 gaattaagcg gaacaaaatt atacttcaat cattctggat caaaacaatt gattcagcaa
    50821 ggtcaaaaca cgttgattta tgagagtgat ggaacagcta aaggagaggt tctcgttgac
    50881 tttcccgata ccgatggttt actcttagag gtaaatagca gtacgaatgc tcaacctggc
    50941 gcaacgtatc aaggcatggt cacatgggaa ctgacagctg ggccaaccag ctaagtgatt
    51001 agaatttgta tggatctgtg ataactgtca cagatcctct ttttcaagga tgagaaggaa
    51061 acttctgctg ttaaaatcag aaatgcttgg aggagtggac ttggaacttt tcagtttgct
    51121 tgaaaaaaat gaacaacggc aaatcaaact tttccaactt ttattagagg aaaaaggaga
    51181 gatttccgtt aataacgtac ttacccgctt atcagttgat cgggctacct tgaaagaaga
    51241 tctgttttat ttacaagcta acttggaaaa tatcaaacaa ttggaaattg ttatccaaga
    51301 agggaatatt ttattaatca gacatggaaa tatagcagca gtcgatgttt attatcattg
    51361 ctacttgcgc tattctacaa aatatcagat acttctttat ctgttggaaa atggctcatt
    51421 tgatcggcag aaacttgtca tggaacttaa tatcagttcg gctactttgt ctcgcagagt
    51481 gaaggaatta aatgattctt taaatgagtt tcatctgaaa ataaaaagcg ggcggctgac
    51541 cggtccagaa agtcaaattt gttattttta tttccaatta ttttggtttg gacgtcctta
    51601 tcttgaaaac aaaaaagaat acagtgattt atcaggaaac acttttttag atctgctgca
    51661 aaaagagttt agttttacta ttacagaaga aggacgattg aaatttgaaa tatggctttc
    51721 cattatgaag aagcgaatgc gctgtacgaa aaaatccaaa ccagattata gcaatcttcc
    51781 tgttggattg tttcaaacga cacaattttt attggacctc catacatttt tgacccgtta
    51841 cttctttcaa tatgcctttc cttggaatca aaatgaaacg ttggtttttt atcttttcat
    51901 gatcagtaac tttacattag atacacataa ctcgcatgtc caggagtttt tgataagcga
    51961 aaaatacaaa gacgacgtag tagctcaatt gaatcgaacg tttaaacaat cgatagaaga
    52021 atatttctct ttttatgttt ttccagaaga gatttcgcaa aagattgcgt tgacagtgat
    52081 gcaaaatcac gctcattttg cctattttag aggatggatg aatatttttg gccaagaagg
    52141 attaaaacag cggttaactg aagtagaaga taaacaattc ctggttttat gtgaaagatt
    52201 ggtcagacaa acatttgcta ttgttgattt tactgaagta gacgaggaaa atgcccgctt
    52261 tgaattattt gctcgctatg catctatttt gcggacgctg atcaagcagt tagaattacg
    52321 tctgcaaatt gcttatgatc ttccttatga aaggctaatg gcggatatcg tgaaagaaat
    52381 catccaggaa caattgctga atacgatcaa atatgattta gtcgaatacg agaaagaaaa
    52441 gcagtatgat atcattttga ctagccaatt gaaagagtac ccgtcacaga agtccgctaa
    52501 agtttttgta ttcactagca acgaatataa ttatgatttc ccctatttga accaattttt
    52561 aaaagaatgt tatttagaaa aattgaattt aaggatgaat aaaacatgaa aatcgtgatc
    52621 attggtgctt cccatgcagg catcacagct gccctaaatc taagaaaact tcaaccgaaa
    52681 gcagaagttt tactgatcga tgaagatcat aaagatggac taggctatgt ttcaaatgga
    52741 atcaaccttt atttaaaagg aaagatacgt agtttagctg aagttgccca taacatgcgg
    52801 actcttcaag aaagtggggc aaaactgatc accgaatgga gagtaactga acttgatcca
    52861 gacaaacatc agctcatcct cgcatccaaa gaaggaaaaa acgagaccat aacctatgat
    52921 aaattaatcg tagcaacagg ttcctctcct gtcaccttgt ataaacaaat tgaagctgaa
    52981 aatgtctaca cctataaaaa tctagtgcaa tctaagcaag tacttgccgc tttaaaagaa
    53041 gcaaaagaag tagtgatctt cggtgctggt tatattggat tggaattagc agatgctttg
    53101 agaaataaag gatacattat tcatctagtt gattatatgc cgaatgtttt atcaagatat
    53161 ttcgataagg atatgatcaa cagttttcaa aatcagctgc aaactaagca aatcaatttt
    53221 tatccaaatg aattcttgat cgattggaaa aaatcagaag agaaagtagt atccgtacaa
    53281 cttctatctc aagcaataaa ggcagatatg gtcatttttt ctgcccaaac tcgtcctaat
    53341 acgacattgt taaaagaaaa agtggctttg tatgaggatg agactgtgat agtcaatgaa
    53401 tatctgcaga caagtgatcc ggacatctat gcgattggag atatcgtccc agtcagtttt
    53461 gacaaaaata aacggcatct ctttttacct ttagttactc gagctgtaca tatggcaaga
    53521 gcagtagcgc taactttgag tggtcagcca accgcttacg acctcagaca aaagataaca
    53581 gcagcagtca ttatagacta ctttttagga actgtagggc tgacagaaga tgaagcgcca
    53641 ttcttggaac aaaataccgg ttcgtgttca ggagaatttg atctatttcc acaatacgat
    53701 gaagaaaaca aaacagtaaa tgcaaagctg atctaccatc cggatacatt agaaataatt
    53761 ggcggacagc tgataagcca agaattctta ttatcagatt tgaacctgct tgcggatatc
    53821 gtgaaacaca aaactacgat tccacagcta gcagttgagg attttggatt tctgtctgaa
    53881 cacacctctc gttttcacta cctcaatgag ttagctttta aagtactagc taagagagca
    53941 aatagcaata gagtagataa aagactgtga gaaatatcac ggtcttttat tgtgtgggga
    54001 tataataaat attaatcaat cgagaataaa aaattcatat aatgaaatag cggaagaatt
    54061 ttttgagcat aatattcgcc aaaaatttat caagtgataa aattgttgta taaaatgatt
    54121 atttcaggag gaggagttaa atgtttttag cattaaatga aattatgcat tcaaagttac
    54181 gttatgcgtt agttgcaggg gtgatgtttt tgatcgccta cctggtattt tttttgactg
    54241 ggttagctta tggcttagct caagataatc gaacagcagt cgacaagtgg gaggcagatt
    54301 cgattgtgct ttcgaaagat gcaaattcaa atctagggat gtccatgatt actaagaaga
    54361 tagcagaaga agtcgaaggt gggaaagtgg cgtatcttgc ccagacacct ggagtcgtga
    54421 ctagtaaaga cagtacagaa gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa
    54481 atcaattcat catgcccaat cttgttgaag gaaaagcttt tgataacgat gatgaggcag
    54541 taggcgatat tagtttgaaa gaagaatatg gtctagccgt aggagataca gttaagttat
    54601 ctggtagtga caagacgttt aaactaactg gctttacgga tcatgccaaa ttcaatgtct
    54661 caccagttct ctatacaacc atcaatgctt accaagagat ccgcttcgaa aaagaagata
    54721 caagtgaaaa tgcacggatt aacgcaatag ttgtcagagg taaaatcagt aatttacctg
    54781 aagatttaga acagatcaaa atttctaaat tcatcaatga attacctgga tataatgcgc
    54841 aagtattgac ttttggattt atgatcggat ttttgatcgt tattgcggct attgtgatcg
    54901 ggattttcat ttatgtactg acgatgcaaa agattaatat ctttggtgta atgaaagcac
    54961 aagggatcac aggaggattt attgcgcgtt ctgtagtggc acaaacattc attttatcat
    55021 ttgttggtat tcttcttggt ctattaggaa cagtgggaac atcccttgtt ctacctgatg
    55081 cggttccatt ccaaagcaat tggctgtttt ttggcgtcat tagcttgttg atgctggtag
    55141 tagctgtgtt aggcgcacta ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg
    55201 caattggata agaaggagga agagcatgaa agcaatagaa tttaaagaag tagaaaaaag
    55261 tttccaagat ggcgaccaag tagttaaagc attgaaagaa acaaccatga gtatcgataa
    55321 aggggaattt gttgcaatca tcggtccttc tggttcaggt aaaagtactt ttttgacttt
    55381 ggcaggcgga ttgcagacgc ctagcagtgg ctctgtattt atcaatgatc aagaattcag
    55441 taatcaaaaa gaaagtgaac gcgttaaact acgttttaaa gaaattggct ttattttgca
    55501 agcttccaat ctggttcctt ttctgacagt aaaaaagcag ttattattag tagataagat
    55561 caaacatgaa catcgtcaaa aagaagcaaa agaattattt gagcagcttg gcgtggataa
    55621 gttgatcaac aagtatccag aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc
    55681 tcgtgcattg tacaatgatc cgtccattat tctagcagat gaaccaactg ctagtttgga
    55741 ttcagaaaag gcacgagaag tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa
    55801 agcaacgatc atggttactc acgatacacg gctgattgac aaatgcgata gaatatttat
    55861 gatggaagat ggtgtattga aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc
    55921 atcgatacaa caaagataca cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt
    55981 ccaagggata tctcggcact tcaacaagag agatcgcaca aaaagttggg atcacccaac
    56041 ccaatctcta tcattatttc ggtgataaag aaaaactata tacggcagtt ttagaaaacc
    56101 acttaaaaga tgttggaaaa gctttgagag aaatcgttca aacaagcgaa acgggatttc
    56161 aagtaacctt gacaaaaatg gcgcagtttt tgattgagac acatctggtc gatttgtttc
    56221 tgatgcttca cgatcttgaa agcaatcttt caaaggaaac aagagatcat cttttctttc
    56281 tatggaagaa aaattatcgt gaaccttttg aagaaatctt ttcagcaaac caatctgtgc
    56341 taagaaatgg gatttcaaaa gaaatcgcag caaggcattt tttcttagtg ttagcacctt
    56401 acatcactcg gtcgaaaaga cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt
    56461 tatatttgaa tggtgtatta ggagaagcgt aaagctgtta tatagggaga aaaatagtta
    56521 ggtgaagaag caagatatga cgaagatcgt tatatcttgt tttttttata taggtagttt
    56581 ttcataaata caaatttgtt attctctcat gattatcagg gtatattttt atttattatg
    56641 tcggtgttcc aggaagtgtt ttaatttaat aacgaagaaa ttaattgttt gtagaatgaa
    56701 ggaaactatt tattgggtag atgaatcacc acgaatagtc aaaaggagat ttcaggatat
    56761 gccaaaaatg aaaaaaatca ttttagcagt aatgttattt tctacggtta tatcttttac
    56821 agcccctttt ttgcgtactt aagctgattt taagttttga cccttagtct ggatctacga
    56881 ggtgagagtc aagaatgaaa aaaataattg gagcttttgt atttgcaatc attatcatta
    56941 ttatctgttt tcaaatagat gtttttggtc aaaaaaagat agttggacaa acggtcagca
    57001 gtacgaacag tacggttaca aagacaaagg aatcatttga tgatacagaa caagagacat
    57061 taatcaaaat cggaaaaaat gaccgtacac tatatacaga tgactcgcta actaccccac
    57121 ttgcaactat aaacggcgga gagctggcgg agtttctttc tgaaacaaaa aatgcttatc
    57181 agatcaagac aaatgatggc tatactggat atttagccct cgaagatgga accaaagtca
    57241 caaagaatat acaaacaaaa ccggaagagc tatctgatgc agtgattgta ttagatcctg
    57301 ggcatggcgg aaatgatact ggggcattaa gcaatgatga acaaacagaa gaaaaagata
    57361 taacattaag tacagccata aaagtaaaaa aggcgctaga ggatgctgga gcaacggttt
    57421 atttgaccca tactacagat gaattagtac agcttggaga tatatgcgac tacagtgaag
    57481 aaaaaaaggc agatgtcttt atcagtttac acgcagactc tacggaatat gcgaatgaag
    57541 caactggaat cacgacatat tattactatg gacaagagga aacattagca cagacaattg
    57601 ctgactcctt cactgatctg cctctggatt caagaggaat ctccactggg aactatcaag
    57661 ttcttcgaga aaatcttcaa ccttctattt taatcgagat gggttatatg aataatgata
    57721 gtgatttaaa ggaattagta acagacagtt atcagcagca gattgctgca agtttgacac
    57781 aagcattgac cacttatttt cagtaataaa taagcccagc caaacaaaaa tagctcctca
    57841 tatttttcgt ttggccgggc ttattatcgc agatgcaagc ttttgaacac cgtttaagga
    57901 agaggttgtg agcctgccgt ttaactccgc gtcacaagga gcaatttcaa aaaacagctt
    57961 ctcatgtttg atgactattg tgacttgtga ccgaggagtt ggcatgagaa caccgtttat
    58021 tcggaacaag ggactgtgac atgacttttg tcacagttcc attatttttt tagcagcttc
    58081 aaagcttttt ctgtattggc aaaatgaagt ttggcatatt tttcaagcat aggcatgccg
    58141 cctttctcca atagttttgc cgctacttgg tcagatttcg ttccagctcc tgatggaagg
    58201 gtgattccag cttcagctga tgctttgtgt aactcttcca aaaagctggc acgactgatg
    58261 atagaagcag cagcaacagc taaatgatat tgttctcctt tagtaacgaa gtacagtttt
    58321 tcttgtaatt gatttttttc taaacgcgca tattttttaa aatttgcttc cggcgtaaac
    58381 tgatcgatca aaatcgcttc tggtttttct ggctcgattt tttttagcag caaatggatc
    58441 gcttgattgt gcaaagcgac tttcatccga acggcattgt attttggttg gatctcattg
    58501 tatttttctg gagataagac aagcagttta taggggatgg ttttttttaa gatgtcagca
    58561 agctggatga tttggctatc ttttaactct tttgaatctc taacaccaag tttacgtaat
    58621 tctgcaagct gatcacgttt cacataagca gcacatactg ttagcggacc aaaatagctg
    58681 ccatttccga cctcatcact acctaaaacg gataattgac tgatattttt tggcaaagaa
    58741 gaggctgttt ttgctggctt ggcttgttta tcctgtgcct ccccccattt atcagcttct
    58801 attgctgcat tcgtaccttg aaacatcact tttccggatg tataagcggt aatggtcgtc
    58861 ccgtcttttt ttgcagcaaa gctagtataa ggaatctgtt tatttaacaa ataagagcga
    58921 tagtatgctt tcatttgact aagttggtcg tttgttgctt tgataatgac gttgctcata
    58981 aaattctcct cgtttttata cataaaacta taccgcaact cacgactttt cgttttctaa
    59041 gagattgcga gacatagcat ttttttaaag tttatcatat aatagttaag tatgtgaaaa
    59101 atatttgtgg agaacggtga aagaaccaac ttcatggtac actaaataaa actttcatca
    59161 aataggagga aatgattcgt atggcgcacg aaaaaacacg ctacaaagca gtgattgcca
    59221 accaaacata tactattatc ggacgggaaa ccaagcatca tatggatatc gtaacgaagc
    59281 tgatcaatga acagttagca gaattgaaac aattatcacc acaaatggat aatgaacaag
    59341 cagcaatctt gatggctgtc aatgcgcttt cggatcaatt gaagaaacaa gaaagaatct
    59401 tggagctgga agaagaaaca gcagaattaa agaaaaaaat gatcaaattt acagagcttg
    59461 aaaatcgtgt gaaacgcatt gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa
    59521 atggtcaagc agatcatacc attaaaaatc atgtggaagc acaacagatt ttaaacgaga
    59581 aacgaaaagg acaaattaaa caaaaatctt ctaattaaga tccttttatc aaaataagaa
    59641 aaatttaaaa aagttagaag agaaaggaac gtagcatgct gagtttgctg attttatttt
    59701 tattgctaat tgcgtttttt tctggagcac gtcgcgggtt tgctttgcaa atcatattcg
    59761 cgattggata tgtattgtca tttattgctg cccagcattt ttacaaacca ttagcaagtc
    59821 atttggaatt atacattcca tacccagctg tcaccccaac atcaaagtta gcattttttg
    59881 atcaggtatt tgcttttcac ctagatgaag ctttttatgc aggaactgct tttctgatta
    59941 ttttgctaat tggctggctg cttacccgat ttgtgggggt ttttgtccat ggattgacgt
    60001 atgtgccgat cttgagacag gtagactgga ttgcaggagg catcctgtca ctaatcatgg
    60061 cttacgttac tatatttttg attttgcagt tacttgcctt tgttccatta gtgtggtcca
    60121 aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc catttcttac
    60181 caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat agtgctggaa
    60241 aatatttcgt tttttgtaca gagtagttta tttaagaagg ctgcgtagcg tatgtccggc
    60301 acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa tccttgaaac
    60361 attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg cacaaggaaa
    60421 agaagaactg gcagaattgg taccagtgag tgagaaacaa acgatcacaa actggcttca
    60481 agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta tacctaaact
    60541 tgaaaatatc cgtccccata tgaaacggat cgagatcgga gcagacttga atggagtgga
    60601 gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt tcattgatga
    60661 tctttctgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc agttagtcac
    60721 gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac gcgtgactga
    60781 tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg aacaagctgt
    60841 tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa gtgatgcgat
    60901 catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc gcggtgtatt
    60961 tggcggtgta gtccacgatc aaagtgcttc aggacagact ttatttattg aaccaaaaca
    61021 agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa atgagattca
    61081 acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa tcatccacaa
    61141 tgcttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg gtaaagaagt
    61201 aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac aagcaagaca
    61261 tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa aagactatca
    61321 agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga agactttagg
    61381 tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag aaagtcaaat
    61441 cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg agcaaagtct
    61501 atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag tcaatgaaaa
    61561 aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag gtgctgctct
    61621 agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg caacaacgca
    61681 ttatcctgaa ttgaaagttt atggatacaa tcgggcaaat acgattaatg ccagcatgga
    61741 gttcgatgtg gatacactaa gtccaacgta tcgtttgctg attggcgttc ctggacgaag
    61801 caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg atgaagcgaa
    61861 gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt tagagaatcg
    61921 ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg aggcacaaga
    61981 acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg aaaaagaaat
    62041 ggaaaaagca aagaaaaaag caaacgaagt cgtgtcagag gcggaagaaa aagcagaaaa
    62101 aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg tgaaagagca
    62161 tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa ctttgaagaa
    62221 aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag acgaagtgtt
    62281 ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc agtggcaagt
    62341 agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg ttgctcctca
    62401 aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta gtcacgtgcc
    62461 gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg tagatcaata
    62521 tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg gaaaaggaac
    62581 aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg tcaaaagctt
    62641 tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat ttaagtaaag
    62701 cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata ctaatcatag
    62761 attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac atttaatgaa
    62821 gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg tccatgtcgt
    62881 atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga attcagaatt
    62941 gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat catgagcatt
    63001 ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg tgttcattca
    63061 aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc aaaaataagc
    63121 tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt taaggttatt
    63181 caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt ttgacagaca
    63241 tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac gaatcaaaaa
    63301 caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata aaaatggaac
    63361 gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat attttactgg
    63421 cagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg aatacatcgt
    63481 tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga aaaatgatcc
    63541 taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga taacgaatga
    63601 aaaatatccc cgtttggtga ttactcgtaa agtactaaaa gacaaagcac tgtattttgg
    63661 accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc gattatttcc
    63721 tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc atttgggaca
    63781 atgtctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa tcgttgatga
    63841 agtgaaaaag tttttaaacg gaggacacga aaaaatcgag tcagagatcc atcagaaaat
    63901 ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag accagatccg
    63961 tgccattgag acaatcatga ctcgccaaaa aatgacaaat actgatttac tggatcgaga
    64021 tgtatttggt tatgcgattg acaaaggatg gatgtgtgtc caagtgtttt tcgtaagaca
    64081 aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag cagaagaaga
    64141 tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc ctaaagaagt
    64201 cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta cgaaagtgct
    64261 gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgaataaga atgcctcagt
    64321 tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga tcggagcagt
    64381 tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt cttttgataa
    64441 ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg atggaaaacc
    64501 ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg atgattatgc
    64561 atcaatgaga gaagtcatct atcgcagata ttcacgagtg attcgcgaag ggttgccttt
    64621 acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa aagatgtctt
    64681 agaaaatcaa ttaggaatcg atattcctat tgctggcatg gcaaaaaacg ataaacataa
    64741 aacaaatgag ttgctattcg gtatagattt ggctgtagta ccgcttgagc gaaattcacc
    64801 agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa tcactttcca
    64861 tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg aaaggttagg
    64921 accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata tcacagcagc
    64981 cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa acgtatttga
    65041 taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg tcctatcatt
    65101 ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg cttacgtctg
    65161 gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag aagtcaagga
    65221 tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc aaatccccga
    65281 tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga cttttcgttt
    65341 gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc atcatcttgg
    65401 ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt atccgaaagt
    65461 aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa gaaaagagat
    65521 tgaaccaaaa atcgattttt ggatggaaaa atttcaggta aaagggaaaa agacggataa
    65581 agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca cgttaatcca
    65641 tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg taaatgctga
    65701 attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca ttttttctag
    65761 ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc gaaacggaaa
    65821 aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta cgaagttatt
    65881 cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga aaaaagtgat
    65941 ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg ggaaagaaat
    66001 ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac cgccaacttt
    66061 ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg attattacca
    66121 aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata ttagtccctt
    66181 ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat agtgatgtca
    66241 ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc caagtcaaaa
    66301 cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa gagctttcgg
    66361 aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata aatgggatgc
    66421 tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa caactgactg
    66481 gcttgcaatc gatgcttcga gcaagtcaat taggcatttc accagaagaa gtagctagct
    66541 tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa ggaaaaatga
    66601 cagttggaga agataactcg acagtccaat atgtcgtaag ttatgtagca acgattgtct
    66661 tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct tctgaaaaag
    66721 gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat ttttacggaa
    66781 aactaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt attatttttg
    66841 gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta gacggtatct
    66901 cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg atctttggca
    66961 ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa gcagaagata
    67021 cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatatatg cttggattga
    67081 tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat attccattct
    67141 tatcttcata tattatgccg gttcgcttag caaatgagac agtcggtatg ggtggagcat
    67201 tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg tctgcaaaca
    67261 tgtacaaatc gaatgtactc gtgtatagcg aaggcggatt atggagttca ttgaaacaat
    67321 cgatttcaat tatgagaaat gaacgaaaaa aagcataaaa tatagaaaaa tatagaagga
    67381 agctggacat ttattgttcc cagcttcttt tttgtataga cctatttcta tttttttatt
    67441 atattggata tatatattcg ttgaaaatag agcataacaa ctttataatg ttgagtatac
    67501 caatttttgg aggcgtttta atggaccgct atttagattt actttcagag aagttcccta
    67561 caacggaaga tgtcatcacg gaaatcatca atctcgaagc aattttgcag cttccaaaag
    67621 gaacagagtt atttttgagc gatatacatg gcgaatttcc tgcttttgat catattctgc
    67681 gtatcggttc aggaaatctt aaagaaaaga tcaaagacct tttctctgaa caaatgacgg
    67741 aaacggagat cagccaattc actattttta cggcttatcc agaatatgtg ttgacgactg
    67801 attggtacaa aaaacaagat aaatcccagc tgatccatcg tttgatcgat cttttacggt
    67861 ttacgacagt caaatacact cgatcaaaag ttcgaaaagg attgccgaaa gaatatagct
    67921 atatcattga ggaactcttg tatatcgatg atcgtatcaa cgggaaaaaa gactatgtaa
    67981 aaaaaatcat tgaacaactt gtcttgatga aggaagaaga aaaattttta acaaaacttg
    68041 cgcagacgat ccaaaaatct gtcatcgatc atttgcatat cgtgggtgat atttttgatc
    68101 gaggaactca agcagccaaa gtaatggatc gaatcatgga tttttcctct gtagatatcc
    68161 agtgggggaa tcatgatatt ttgtggattg gtgcgtattg cggctctgag gcatgtttat
    68221 tgacgctttt acggattgcc gcacgctaca actatttatt tgaattagag aaagaatatg
    68281 gattaaattt acgtccatta ttttcttttg cccatcaaac ctatcagaag aatccagtat
    68341 ttacccctaa aaacaggaaa gatctatctg aaagagagca agaagaatta gaaaagatcc
    68401 atcaagcact ttctatcctc caattcaaat cagaacatca gttgttgaat cgacgacctg
    68461 aatttaaaat ggacgatcgt aaattgttgg aaaaaatcaa ctatgaggaa tcaacgatcg
    68521 ttttagaagg tcagctctat gagttgaaag ggacttgttt tcaaacgatc cagccagatg
    68581 atcctaaaaa attcttggaa gaggaacaaa aagtgatcga tagtttgatg tattcattcc
    68641 agcactcgtt aagaatgcaa aaacacatga cgtttttgat cgaaaaaggg ggaatgtatc
    68701 tcacgaacaa tcagcatgta ctgttccatg gctgtatacc tgtagatgaa aaggggcagc
    68761 tcttagcatt tgaattagac caatcttatc gcggaaaaga actgctggga ttttttgaaa
    68821 aacagataac tgaaagcgca aaagatttga ctgcaaatga agatttggcg accgatttga
    68881 tttggtatgc gtggagcggg ccattttcgc ctttattcgg caagagtaaa atggcaactt
    68941 ttgagcgcta ttttctcaca gagcctcata cgcacgtcga aaaaagcaat ccctattatc
    69001 acttgcgaga tgaagagtgg ttcagtgaaa aaattttggc agaattcgat gcaaaagaag
    69061 aaggttcagc aatcatcaat ggacacacgc cagtaaaagt aaaaaaagga gagtcaccga
    69121 tcaaagcaaa cggaaaagca tttgttattg acggaggtct atccaaagct tatcaaaaaa
    69181 caaccgggat tgctggctac tcattactat gcaattctta cggtttccaa atcgtgacac
    69241 attatccgtt cttgcctatt gaacaacaat ttgcaaaaaa atctgatcaa acgaatgtaa
    69301 aaaaagtgat cgagcagcag ctcccacgca aacttaatcg gaatacgaca aaaggaatgg
    69361 aattacagca acagattact caactccaaa ggctgttgga ttatcgaaag gacgattgag
    69421 aaaaatgcta gccaacattg ctcaatagac taaaaagcga ctgcaacaaa aggcgtgtcg
    69481 cagtctcctt cggaattagc tcaaaaaacg gtaaaatatt agaagctatt tttctgttta
    69541 tttctctaat actcaggtca gggcgctgat ttcacgacct cttccttaaa cggtgttctc
    69601 ctgccaactc ctcggtcaca agtcacaata tccaacaaac atgagaagct gttttttgaa
    69661 attgctcctt gtgactcaga gttgaacggc aggctcacga cctaatcaga aaaacgatta
    69721 aaattcattc acaaagaaat cgaataaacg ctgttcagat gaatctacac gatgagaaag
    69781 ttccggatgc cattggacac caaggatttt gtgcatatta tctttacttt ggactgcttc
    69841 aatgagtcca tcgttggaaa aggcgatggc ttttaacaaa ggagaaagat ctttgactgc
    69901 ctgatgatga taggaattca caaagtatga atcagggaga aggtcactta ataaactgtc
    69961 cgaaacgatt ttgacttcgt gagttgcaaa ctgcggggca gtgggctgtt gttcgtgttt
    70021 tacggaccat tttggatata atgaaagatc ttgataaagt gttccctcca acacgacatt
    70081 caatagttga agtcctcggc agactgcaaa aatcggtttt ctttgtttca atgcttccaa
    70141 gatcaatgcc tgttcgaatt ggtctcgttg aatatttgtt tccgtcagtt ttggatgagg
    70201 gtcttccatg taaagttgcg gactaacatc ctgaccgcct gcaagaatga gtttgtcgat
    70261 tttctcaatt agttgaggcg ctgaatctgg agacatgact ggcagaataa gaggtaatcc
    70321 ttgagcatct aaaactgcat ccacaaatcc ctgcggtgta tagcttactt gatttccttg
    70381 gaaagtatct gtcgctcgaa tcagttgatt cccggcaatg ccgatgattg gtttcatttt
    70441 tattccccct aaagtagact aattgtaaca aggatttaaa aaaatttaaa ctatgtaaga
    70501 cttgccccta tacttatctg ttcttgctgt gttactatct gtaaagagaa ttttcgtgtg
    70561 aaattcatga cgatagttga aaatattgct taatttgtta taataaaaag taagcagaaa
    70621 taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca aggggtgaga aggacatttt
    70681 attgaagggg cctgcctata ctaagttatc tttagaggta catactttca atgaaaaaag
    70741 aaagcatgta cctctttttt aaatcgaaag gaagtgaagc aagtgttaga agctttggaa
    70801 caaatccgaa aagcagaaga agaaaatgag aagcgtgctc gaaccctacg gcagcagttg
    70861 aagacctatg cagaagaaaa agagcaagaa ttggagataa tccaaacgaa cagtcggcaa
    70921 cagctgaaac agttggtaga agaaaaagaa caattcgaaa agcaagaatt agaaaaagaa
    70981 aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga ccaatatcaa
    71041 aagcatcaga gtcaagcaat cggtgcaatc attgaaaggg tgaaagaaac ttatggccgt
    71101 cactaagatg gaaaaagtga ccctcatttc agataaaaga aatcaggaga ggattttaca
    71161 agcagtacaa ggaatccaaa atgttgaaat ccgggatctg tatcaaaata gaacaaacaa
    71221 ccagtgggta gaaaaatatt ttccagatac cacgattatt gataaagaag caaaattaag
    71281 cgctttaggc aatcggctga aagacatcca agaagctatc caattcatac aacaccacgg
    71341 cgatagccgt caaaagaaac tccatttgaa acgtcaagaa ctttccttac aagacttgga
    71401 agcaaattat tccgaagaaa atttcatgaa taaattagaa gaagtccttg atttaaaaga
    71461 aaagtgggaa cagctaactg aaaaacgtga tcgtttagaa gaagaggaag aatggcttct
    71521 tcattggcaa cagctggata ttgctccaag tatgtatgca agtaagacga ccatttttga
    71581 gatggggact gtgagtgcag caaattttga aagtttcaag gaagaactaa gtaagcttga
    71641 tgacgtttat atggaagaaa tagattatac gccaaaacaa gtttatcttt cgtatgttgc
    71701 tttaaaaaaa tcagttgctg ctatagaaga aattgctggg aaatacgggt tcaataaaga
    71761 aacgtatcct tatgaacaaa atcctaaaga aaaattgcaa gaaaccaaac agcaattatc
    71821 cattgttcac gagcagcaaa aaaagataac gacagcgtta ggtcagtgca gcggctatat
    71881 ccaagaattt gaatgggtag aagaagtcac acttgccgtt gcagaacggg aaaaagtaaa
    71941 agaacggttt atccatgcca cttatctgat cgttttgcaa ggttgggtag atgcagaaga
    72001 aaaacagaat ttgatgcatg tgttaaacga aactgtatca gaagaagata tatacgttag
    72061 tttcgaaaca ccaaactcag aagagattca gcaggaagtc cctactaaat tgaaaaacca
    72121 tccgcttatc gaaccttttg aattgctgac tgaaatgtac agtctgccaa aatatgaaga
    72181 agtagatcca acgccttgga tgacaccatt ttatcttgtc ttcttcggga tgatggtcgc
    72241 agatgttggt tacggattgc tgatgctcat tgggacaata ctagctcaaa aactatttgt
    72301 tttaccccgc gggatgaaac gtttcgtaaa attctttgag attctctcgg taccatcgat
    72361 catctgggga ttgatctaca gttcattttt cggacaggca ttaccaaaag aactatttgg
    72421 tatccgcttg cctttcccaa tcctatctac gacggatgac gtcaatacga ttttgatttt
    72481 atccgtgatt ttcggtctga tacagatctt ggttggatta ttcgtggcag caaaggaaaa
    72541 tatcaggagg aaagattact tgagtgctgt cagtgatggt tttgcctggc aagggatcct
    72601 gattgggatc gttattgcat tgattggtgt catgttgata aaaaatacag catttgttta
    72661 tcttggcgcg gggctatcga tcattgctgc tttatgtatc ttaatcgtgc cgattatcca
    72721 atcgccgtct aaagtcaagg gtgctgccaa aggagcgtat aatctttacg gattgactgg
    72781 ttatatcggt gatttagtca gttatacacg tttgatggca ctaggtattt caggcggaag
    72841 tattggcgcg gcattcaata tgctagttgc tttcatgccg ccagccgctc gattcagtgt
    72901 agggatcctt ttgatcatcg ctttacacgc actgaatatg tttttgacac tgttaagtgc
    72961 ctatgtccat ggtgcacgat tgcagtatgt cgaatttttt ggaaaatttt atacaggagg
    73021 cggacgtgcc tttgacccgc tgaaaacggc ggaaaaatac gtcaacatca atcataagaa
    73081 aaaaaacaat aaataattgg aggattttta tacaatgatg gattacttaa ttagtcaaaa
    73141 tggtggaatg gtatttgcag tattagcgat ggcaacagcg acgatttttt cagggattgg
    73201 ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca gcgttaacaa caagtcaacc
    73261 cgaaaaattc ggacaggctt tgattctgca attgcttcca ggtacgcaag ggttgtatgg
    73321 attcgttatc gcctttttga tttttatcaa cttaggaaat gatatgtcag ttgtacaagg
    73381 attgaacttt ctaggcgctt ctctgccgat tgcttttaca ggtttgtttt caggtatcgc
    73441 ccaagggaaa gttgcagcag caggtatcca aattttagcg aaaaaacctg aacatgctac
    73501 aaaaggaatc atttttgctg cgatggttga aacatatgcg attttaggtt tcgttatttc
    73561 cttcctatta gtattgaatg cctaagcata aaggaggaaa agcagatgaa cgccattgaa
    73621 aaaatcatct ctcagatgaa tgaagcagca gagcaagaaa gagcggcgtt agagcaagaa
    73681 gagcgaatga agatcgatca aaactttgaa caaaaacgta cgcaaatcga aacagaacat
    73741 caaaaacaaa aagaaaaaca aatcgaatta cttgaaaaaa aataccgtca gcttaggaat
    73801 cgccaacaag tagaagtacg acaagaaaat ttgaatgcga agcaggaatt tttacgacga
    73861 ttgtttgcag atgcaatagc tgaaatggag aattgggatg aatctgaaca aatccagttt
    73921 atcaaaaatt cattgtattc attacctttg acaggcaaag taacattcat tgctggagaa
    73981 aaatcagcag cttatctttc tcagacattg ctggatgaat ggaacaatga acttcccttt
    74041 atgatggttt taagtgatga aactgttgct gatcaagcgg gttttctgat aaacgatcaa
    74101 ggagttcagt ataattttct tttcagcagc cttgtacaag atatccaagg aacgatgagt
    74161 tttgagattg ccaatcaatt attcgaataa ggagggatca gtgtatggaa taccacgaac
    74221 taaatccctt gattcgcgga agagagcttg aacttttgtc caatgagaca tttgaacgga
    74281 tgattcaaac aaattctgtc gaagcacttg gtgaaatatt gaaatccacg atttacagtc
    74341 catatattta tgacggatat gaatatgact tcgaacagaa tcttttaaag gagcagagcc
    74401 gtctatttcg ctggttaaag gaatcagcac cggagcctga aatcgtttgg atctatacga
    74461 tgcgctatac tttccataat ctgaaggttt tgacaaaagc cgagatgaca aatcaaaatc
    74521 tggaccattt gtatatcgac gatggatttt attcattaga aaccttgaag gatgccattc
    74581 atacgcaagt ttcaaatgaa ctccctgaaa gcattatgga cagtatacga gaagtacgtg
    74641 attattttga agagtctacc attcttcagg gaattgacgt gatttatgat cgacagtttt
    74701 tgacagagca gcgacggctt gccgataagc taggatatca agaattgctg acagaagtca
    74761 ttgcttttat tgacttgacc aatatcacaa cgatggctcg aggaatattg cagaaaagaa
    74821 catatgggtt catgacgacg gtactttcca gctcgggaag tatcgaaaaa gaagtatttt
    74881 tgaactttgt agataaagat atggaagatt acacgaattt tctattagag acagattatg
    74941 ctgattttct gcgccctgtc atccgtgagg gaaatcttga tctcgtaaag cttgaacagt
    75001 tgaaagatga ttatctaagt tcattgtatc aagaagcaca aacccaggct ttcggaccac
    75061 tgccactact agcctttttg aacgcaaaag aggtagaaag caaaaacctt cgtttactgg
    75121 ttatcggcaa aagaagccat ttcgaaccag aaacaatcag aaagcgggtg agacaagtct
    75181 atgacgcata agatcggtgt agttggcgac aaagattctg ttttgccatt caaattgttt
    75241 ggctttgatg ttcgttatgg tacaaccaag caaaccgttc gtcaggcaat tgaagaaatg
    75301 gcaaagaatg agtatggcgt gatttatgtc acagagcaat gtgcagcatt gagcccagaa
    75361 acgatcgaac gttacaagga acaaatgata cctgctatcg tattgattcc caatcatcaa
    75421 ggatcattag ggatagggct ggaagaaatt caaaaaaacg tagaaaaagc agtaggtcaa
    75481 aatatcttat agtatgaaaa ggagatagaa attttgcaaa ttggaaaaat cataaaagtc
    75541 tccggacctc ttgtaatggc tgagaacatg tctgatgcca gtatccaaga catgtgtcta
    75601 gtgggagatc taggagttat cggagaaatt atcgagatgc gtggcgatgt ggcgtctatt
    75661 caagtatatg aagagacttc tggaattggt ccaggcgaac cggttcgctc tactggtgaa
    75721 gctttatctg ttgaattagg accagggatc atttcacaaa tgtttgacgg aatccaacgg
    75781 ccattagata cctttatgga aatcacacaa agtaatttct tagggcgcgg agttcaattg
    75841 cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa cagtagaagc cggagaaacg
    75901 gtgagtgctg gagatgttat cgggatcgta gaagaaacaa aagttataaa acataaaatc
    75961 atggtgccaa atgggataaa aggaacgatc aaaacaatcg aaaaaggttc attcactatt
    76021 gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca aaaatttgac gatgcttcaa
    76081 aaatggccag taagacgtag tcggccaatc aaagaaaaat taaacccgga tgcacccatg
    76141 atcactggac agcgagtgat tgatacattc ttcccagtga caaaaggagg agcagcagct
    76201 gtacctggtc cttttggtgc agggaaaaca gtcgttcagc atcaaattgc aaaatgggcc
    76261 gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg gaaatgagat gacggatgtt
    76321 ttaaatgaat tccctgaatt gattgaccct aatacaggag aatcgttgat ggaacgtacg
    76381 gtattgattg ctaatacatc aaacatgcca gtagcagctc gagaagcttc tatctatacg
    76441 ggaatcacaa ttgcggaata tttccgtgac atgggttatg acgttgctat catggcagac
    76501 tccacttcac gatgggcaga agcacttcgt gagatgagcg gacgtttgga agaaatgcct
    76561 ggtgatgaag gttatccagc ttatcttggt tcacggcttg cggaatacta tgaacgatct
    76621 ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa gtataaccgc aatcagtgct
    76681 gtatccccgt ctggagggga tatttcggaa ccagtcacac aaaatacatt gcgggtcgta
    76741 aaagtattct ggggtcttga ttccagttta gcccaaaaaa gacattttcc ttcgatcgat
    76801 tggatacaaa gctattctct ttattcaaca gaagtaggga agtatatgga tcatatcctt
    76861 caagctgact gggcatccat ggtcaccgag ggcatgcgta ttttgcagga agaacagcag
    76921 ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt cagataatga ccgtctgaca
    76981 ttagaagtgg caaaatccat tcgtgaagat tatttgcagc aaaatgcttt tgatgatgtc
    77041 gatacattca cttcaagaga gaaacaattc aaaatgttga agatgatctt gacctttggt
    77101 gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa gtgagatcat ggacggtacc
    77161 gtatcgattc gtgaaaggat cggacgaagc aaatatatcc cggaaacaga attagaaaaa
    77221 ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa cgatcgtttc agaaggaggc
    77281 atgagcaatg attaaagaat atcgtacgat cggtgaagtc gtcggtccat tgatggcggt
    77341 tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa gtccgtatgc aaaatggcga
    77401 gattcgtcgt ggacaagtat tggaagtcca agaagataaa gcaatggtcc aaatattcga
    77461 aggaacaagt ggtatcaatt tacgtgactc ttctgtacga tttttaggcc atcctttaga
    77521 actaggtgta tcggaagaca tgatcggtcg agtattcgat ggtttgggac gaccgaaaga
    77581 taacggacca gagatcctgc ctgaaaagca tttggatatt aacggagaag taatcaatcc
    77641 agtagctcgg gattatccag atgagtttat ccaaacaggt atctctgcaa tcgatcattt
    77701 gaatacatta gttcgtgggc aaaaattacc agtattttct ggctctggtc ttcctcataa
    77761 agaattagct gctcagatcg cgcgacaagc aacggtactc gattcatcag atgatttcgc
    77821 cgtagtcttt gctgctattg gaatcacttt tgaagaagct gaatttttca tggaagattt
    77881 tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg aatttagcaa acgaccctgc
    77941 aatcgaacgt atcgctactc ctcgtatggc tttgactgct gcagaatatc ttgcttatga
    78001 aaagggaatg catgttttgg ttatcatgac agacatgacc aactatgcag aggcattgcg
    78061 agagatttct gcagcacggc gagaagttcc aggaagaaga ggatatcctg ggtatcttta
    78121 cacgaactta gctactttat tcgaacgagc aggaagaatc cgcggactaa aaggatcggt
    78181 tactcaaatt ccgattctaa caatgccaga agacgataaa actcatccaa tccctgactt
    78241 gactggttat attacagaag gacagatcat cttgactaga gagctttaca agagtgggat
    78301 tcagccacca attgatgtac tcccgtctct ttcgcggtta aaagataagg gaactggacc
    78361 cggaaagaca agagaagacc atgcagctac gatgaatcag ctttttgcag cctatgcaca
    78421 aggaaaacag gcaaaagaat tagccgtagt tttaggtgaa tctgctttat ctgatgtaga
    78481 caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa tatgtcaatc aaggattcta
    78541 cacgaaccgt tcgattactg aaacacttga tctagggtgg gaactgctat cgatgttgcc
    78601 aagaaccgaa ttaaaacgaa tcaaagacga catgctggac aaatatctgc cagaagggaa
    78661 gtgagttctg atggcacgat tgaatgtgaa tccgacccgc atggaattga ctcgattgaa
    78721 aaaacagttg acaactgcca caagagggca taaattatta aaagataagc aagatgaact
    78781 gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag ctgcgtcaag aaatggaaga
    78841 gtctatgaca gaagcaatgt ctgattttgt attggcaaat gcttcgatca cggaaccttt
    78901 catcgaagaa ttatttgttt tgccggcgga aaacgttgat ttgtctatcg tagacaaaaa
    78961 tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat gatgaacagc tgacagacac
    79021 acctttggaa tacgggtatt tgaattccag tgccgaactg gatcggtcaa tcgatcggtt
    79081 tactaccctg cttccaaaac ttttggaact gacagaaatc gaaaaaactt gtcaactgat
    79141 ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca ttagaatata tgacgatccc
    79201 tcaattagaa gaaacgatct attatattcg tatgaagctg gaagaaaacg agcgcgcaga
    79261 agtaacaagg ttgatcaaag taaaaaatat gggtaccact gactaagtgg tatctttttt
    79321 taaaagagga aaacgatgat taaacaaagc aaacaaataa agaaacgttt gccggctgtt
    79381 cagcttctcg cattaggatt ttttctcctg attttagttg gcggcggcct acttacccta
    79441 ccatttttta gtaatagtgg gcatgggaca aattttatcg atgcgctttt cactgcaact
    79501 tctgctgtct gtgtaacggg attgacaaca ttgaatacag cagagcattg gaacagtgct
    79561 ggtcaatttt tgatcatgct tttgattgag atcggcggtc ttggttttat gatggttccg
    79621 attctatgct ttgcattgat aaagaagaaa gtcagtctca gcacacgtat tatattaaaa
    79681 gaagcgctga atctagaaga agtatcaggt gtgatgaagc tcatgcttta catcctaaaa
    79741 ttggcctttg ttatccaatt gattggtacg gttgctttat cttttgtatt tgtgcctgaa
    79801 tttggctggt tgaaaggtat ctggtacagt ttgttccatg caatctctag tttttgtaat
    79861 gcagggtttg atttgttagg agacagttta gccaatcacc agcaaaatgt ttatttgatc
    79921 atggtggtat ctgctttgat catttctggt gggttaggct tcatcgtatg gcgtgattta
    79981 ctggaatatc atcagaaaaa gaagatgacg attcattcaa aaatcgcatt aaccgttaca
    80041 ggtacccttt tattaggcgg attcgttgtg ttctttttca cagagcgcaa tggggcaacg
    80101 ctagtggaag ggaatatgtt tcaacgattg gcaaacacct tttttatgag tgtgacacct
    80161 agaacggctg gatattattc agttgattac ctccatatga gccatgcagg tctgatttta
    80221 acgatgtttt tgatgtacat cggcggaaca tctgggtcga cggccggcgg attgaaaaca
    80281 actacgttag gtgtgttgct gatccagatg cgttcgatgt tgaaaggacg tacgagagcg
    80341 gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc gggcacttac tttatttttt
    80401 gttacgttga cgttgtgtgt agtcgccatc atgattttat cggtgactga aacgatacct
    80461 aaaacttctg gcatcgaata tattgctttt gaggtcttct ctgcgtttgg tacagtaggt
    80521 cttacgatgg gattgacgcc agacttgact gcaataggga aattgatcat tatagcgctt
    80581 atgtacattg gacgagttgg tgtattgact gtggtcttct ctctgatgac aaaagcacaa
    80641 agaacacaat caaaatacaa atatccagaa gaaagtgtca tgattggata atttcatctt
    80701 ctgacagaaa cctagtgatt tagtaaaata agaaaataaa ataatttttc ttgcttcttg
    80761 gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca ctacccgttg ttgaacctcg
    80821 ggtagtgcct cttttttatt gaaaggaatc aataagattt gattccttta tttatcaata
    80881 ggttataatt catatttttt tattttttct tttacgatac ttagtccaga agctaaaatt
    80941 tcaaggttat tagaggatag ggaaattcga acgaatgcat ctggttgcgc acgtgtagta
    81001 aagcgtctgg aatgataaag tcggataccg ttagcaagaa agtcattctc aattatctta
    81061 gggtcaatct caggatcaac tggaagaatt cgatagtaag ggacacgatt cgttggtttt
    81121 tctactttga aaagagaatc aaaaagctga ttggctcttt ttgtaagact taatttagtt
    81181 tctaagatcc tatttgcttc atttgaatgg aggatttgag tgatgacttc cacatctaat
    81241 ccagaagttt tcacatttat attaaaaatt gcttgttcta tcttgctttg gacatgtact
    81301 ggaactacaa gataagcaac tcgcaatcca gaacaaataa attttgtcat tccagctaag
    81361 tagattgttt ggttaggtaa taaattttgg aaaggaggaa tgatcgtatg ttgatagtaa
    81421 gttgttaaaa atgaatggat atcatcttca ataacccata gaccttcttt ttcaatcact
    81481 tcggctaatt gctctcgtct tttgtgagag atttgaaacc ctataggatt attacaagaa
    81541 ggcataagaa agacaccatg aatcttcttt tttctacatt cctgaatcaa tatatcaggt
    81601 cgcattcctt gctcgtcaaa atcaattgga acgatctcta aatgatataa ctgagccaat
    81661 tcaatgaaat tagcgtatgt atatcggtct gttgctattc tatcacctgg agagaaaagt
    81721 gcagaaagtg tgatagccaa tccattttga acaccggaaa caatagatag agattctaag
    81781 gacggaatga ctccttgagt ttttaaccaa tttgctgcag tagtcaattg aaaatttgtg
    81841 cctttgggat cttggtaatt aagcaaacca acaagatttg ggttattact agttgattga
    81901 ataaacggga caacgaattt attacattct tcaaaagaac taaccagacc aaaatcaatg
    81961 atatgttcgg ttacttcatt ggtagagatg gtgatggact caaagacatt gggagaaata
    82021 aatgtgccac ttccaatatt cgtatacagc aaacctttct caattccata tttataagcc
    82081 ttgccaacgg tagtaaaatt taaatctaaa taatcagcta gctctctttg ggagggtaac
    82141 tgagtgtttt tctgtaattt attctttaaa atatcttgtt ctaattgttg aatcaacgat
    82201 cgataataag gacgcttcaa tttttgtttg tctggtcgcc aagttaacgg gtaattgtca
    82261 taagaattaa taggcatttt ctccactcct taaaattgta taccatacaa ttttaccatt
    82321 gattgtattg attttagaag ttaatctttt gtgaggaggg aattgtctat gaaaattatt
    82381 aattttgagg aagtagtagc ttcttttagt tttgaagaag cgattcaagt aatgaaaaaa
    82441 tgtttttttg attatgaaag tggtaaaatc agccaaaatc ctcgattggt ggaaatttta
    82501 cctgacggaa aaggcaaaaa tatgtttgcg atgatgcctg cttatttggg ggagaacaga
    82561 tattttggca caaaagtcat taccgctttt ccggataatc atcagtatca tttaccttct
    82621 catttaggag agattttctt gtttgatgct caaaacggac tacccaaagc attgataaac
    82681 gcaaatgcag tcacctggat tcgaacagct gctgtatctg cgctggcaac agattactta
    82741 gcaaatccgc atgcagaaac acttgcttta attggagctg gtcaacaagc attgtcgcat
    82801 ttgactgcga tttctaccat tcgtcccatc actacagtgt ttgtttacga tattttagaa
    82861 gaaagacgaa atacttttat cgctaaagct aaaaaacagt atcctaaaat aacgtttata
    82921 aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa tcatttgtac gttgacctct
    82981 agtaaagagg catttcttca aaaagagtgg gtaagggaag acgcacatat caatgctgtg
    83041 ggcacattta ctcctgatac aagagagata agtacatcgt tgatggaaac ttctcaaatc
    83101 tatgtagatg attactcagc tgccttaaca gaaagcgggg acctgcttat agccatggca
    83161 gaaggagatt tagaggaatc ttgtattgtt ggtacattag gcgaattagt ttctgagaaa
    83221 aaaacatttt ataaaggaga atcgaggccg acgattttcg acgcagttgg tctagctatt
    83281 gaagatcttt gttgtgcaga atatatttat aacaaattag agaaaggggc tttaaaatga
    83341 acttagatta ttacgtagtt gatgcatttg cagatgaggt gttcaaagga aatccagcag
    83401 cggtttatgt gctagaagaa tggctgccag aaggtacgat gcaaaaaatt gccattgaaa
    83461 acaatttatc tgaaacagca tttacagtga agaaaaacca agaatttgag cttagatggt
    83521 ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac attagcaaca gcatttgttt
    83581 tatttaacta ttacaaaata cctgatgaaa cgattaaatt ttctactcaa agcggtaatc
    83641 tatttgtgac aagaattaaa gattattatt acatggattt tcctagtatt atgccgaaaa
    83701 aagtgccgat attgtctgaa tatgaagaag cgattggggc taagattaaa gaagcctact
    83761 tagcacgtga tttatttttc gtattagaag atgaaaaaac ggtggctaaa ttgcagccag
    83821 attttacagc tatcaaaaat tttgaattag gaataggagt catcgtgaca gcagaaagtg
    83881 tgcaaaaaga ctttgtttct agaaccttct ttccaaaatt aacaatcaat gaagatccag
    83941 tatgtggatc agcacattct aatcttattc cttattgggc agaaagaatg aataaaacaa
    84001 aattgtcagc ttatcaaatg tcatcaaggg gcggggatct agaatgtgaa ttaaaagaag
    84061 atcgtgtgat aataggagga aaagcagttt tgttttctaa aggtacctcg tttatttgat
    84121 ttctttcaaa tgaattaaaa ataaattggt ggagagtggc aaccttatag ctacaatggt
    84181 tgttaaaaaa agacgcaaac tttttcaatt taatgaaaag tttgcgtctc attttaatta
    84241 ttggtgtttg ttttttattc aatcaagagt aaaatgcccc atactaacta tcctcggatg
    84301 acttcgattt tataaccatc agggtcaatg acaaagtagt aagaaggcgc agtgccaggt
    84361 aatcctttta agtcagtaat ctcaagaccg gcagctttat gttgctcgtg taatttttct
    84421 agatcatcag ctgctatagc gatatgacca tagccgtctc ctagatcata cgcaccatga
    84481 tcatagttgt aagtcaactc taattcatag tcatccccag gaagtgtcaa ataaacaagt
    84541 gtaaatttgt tttctggaaa atcacggcgg cggctttctt caaaaccaaa tgccttttga
    84601 taaaattcga ttgatgcttc aagatctttc actcgaacac atgtgtgagc cattttcata
    84661 gattcatctc tccttttcct tttactcaat cataacaaaa agaagtaagg aaatagttga
    84721 tttgtgcttg ttcatgagaa atttctcatg taaaatggat agaaaataaa actgttaagg
    84781 tggaaactat tcatggataa accaattttt ggtgaaaaaa agcaatttgc tgattatcaa
    84841 acccgttatg cagcatatat tgtcatagaa agagatcagc agattgcagt aatcgaggct
    84901 ccaaatggtg ctttttttct tcctggcggg gaaattgaag gagaagagac gaaagaagaa
    84961 gcgattgtcc gtgagctgat agaagaaatg ggaattgtgg cagaaatttc ttcttatctg
    85021 ggagaagctg atgagtattt ttactcgaat tatcgtgcaa cttattatta taatccagga
    85081 tatttctttg ttgctgattc ttggaagcgt ataggagaac caactgaaaa gaccaataaa
    85141 atttggtggg tgagccctca agaagcgctt gtgaaattga aacgtggtag ccaccgatgg
    85201 gcagtcgaga gatggatcga aaaaaataaa aactgaaaga aagcgagtag atcagctttc
    85261 tctcagtttt tttatttatt acagtacata ttttttgatt gcttcagcta caccgtgctc
    85321 gtcgtttgat gaagtgatga catttgctgc ttctttgaca agagaaacgg catttcccat
    85381 tgctactcct agaccagcat attcaatcat ggataagtcg ttctcgttat ctccgatagc
    85441 catgatttca tcttgatcaa taccaaggtg ctgtgcgaga ttcgcaactg ccgcaccttt
    85501 acttgcttct ttatttagta cttcgaagta atagggcgca cttttgaccg ttgtgtattt
    85561 atcacggaaa gtttgcggta aacgcgcaat tgctgcatct aaaatttctg gttcgtcgat
    85621 catcatcatt ttgacgatgt tcatgtctgg tgtcatttct tcaggtgttc gatattttag
    85681 cggcatcttg actaaacttg cttcgtggat cgtataagcg ctgatatctc gattaggcgt
    85741 gtagatcgct ttgtcatcga tcgaatggag atgactgccg acacggcgtg ccatcacttc
    85801 gatctctagg aaatcttcat gtgtcagtcc gtgacgggcg atgattttac ctgatttcgt
    85861 ttgttgtacg agtgccccgt tataagtaat aacgtaatca ttatcttgaa aaaggttcaa
    85921 ctcgtttaac tgttcctgga cacctggtaa gggacggcca gtgcataaaa cgatcgaaac
    85981 accttttgca acagcagcgg caatcgcttc ttttacttct tttgttactt ctcttttctc
    86041 atttaataaa gtaccatcga tgtcgatagc gactagtttg atagacataa tttcctccaa
    86101 tatattttaa tttatttcaa tcaatgcgcc attacggata tggctggcaa attcttgata
    86161 agtttcgtca aataaatcat aatgatcacg caaggacgga tcaaccattt cttttggaaa
    86221 gaaaaatcgc tcatctcctt gttcttgtcc cgctaatgca gcaacaagct gactaacctg
    86281 tgataattct atcaaagttc cgtcatttcg caaaatctcg atttgtgtgc gatgtctgcc
    86341 ttgttctggg cgataaaaat cataaggtag atcgtagcta gaattcacag cagtgtaata
    86401 aacaggatta tatccgacct tttctactaa caaagtgagt tcttggacta attttgaatc
    86461 ccggttgcca gaaaaagtag cagatttcag tggtttacga ttcaaaaaac gtttagctaa
    86521 atcatttaaa ataggatcag cagattcaga ccattgagta aagtaagtgc ccaagacccc
    86581 atcatcaagt ttgaggtatt cttctaaagt aaagcttccg tcaagaaatg gcagaagtaa
    86641 ttgagaatgg agttcaaaac tgctggcatc ttcttgatac aactcgtgtg cgcgatgaag
    86701 cagatgctcc aaaattactt ccataccacg ggaaacagga tggaagtaca cttgcacata
    86761 catttgatat cggctaacga tataatcttc cactgcatgc atgccactca tggaaaaagc
    86821 gatcccgcct tcataaggac gaatcacacg taatattcgg gtaagatcaa atgttccata
    86881 ttctgttcca gtgaaataag catcccgaag caaatagtcc attcgatctg catcgatctg
    86941 actagaaatc atttggacaa cttgtgggtt ggggtaagtt ttttggatca cactagctac
    87001 cttttcagga aaaccttctt ctactcggtt aagaatctga tacacttctg tttctggaga
    87061 agtgatgatc tctacagtga tggcttcatg attcgtatgg aagatatgtt caaatgtatg
    87121 agagtaagga ccatgaccga catcatgcaa cagtgccgca cataaagtca ccagccgttc
    87181 ctgatcatcc cagcctccgt tgcctatttt ttcttttgaa aaatttcgag aaaagatatc
    87241 gcagattcgt cttgaaatct cataaacgcc taatgaatga gtaaaacggc tatgttctgc
    87301 tccgtgaaaa gtgaaagaag aagtgcctaa ttgtttaatg cgtcgtagac gttggacttc
    87361 tttcgaattg atcaaatcta agattacttg atgctggaca tggatatagt tatggactgg
    87421 atcacgaaaa actttttcca taggcagcat ttgatattta taaggggttg tcataatgta
    87481 gctcctttat gttattacgt tgttccattc tctggatttg gttgttccat tgctccagct
    87541 ggatttcttt ttttagccag tcggtagaat cctgtaaatc acttgcagaa tacagctttt
    87601 caaaagcttt tgcaaatgct tctttgactt cttttacaaa aaggggttgg tcaagcagtt
    87661 cttcaagtgt tgccattgta gcaggatcca ctgcaggata gccatttgta ccaaagtctt
    87721 cttttaaagc agattgataa aatcgcttca tgacttgccc gcgtttttcc tgattaccgt
    87781 tgacacttaa gtacatcata acagaaatcc catttttgat ccgacgctga gcgataccgg
    87841 caaatttttt gccatggatg cttaaatcat aagttccagg acaataagaa tgggtgattt
    87901 cgaatgcttg tatcggtgcc tttggaaaag cgtatttagt caataggagc atttgctcgt
    87961 aagcgtcatc tatggttatt ttttttgtgt cactttgggg gaaaatcaaa gaaatattca
    88021 aaattccttc atctgatacg atccctaatc cacctgaatt tctgatcact ggagcgtaac
    88081 ctgcatcttt cagtgtttgg atcccgtcac ttagaaaagg agttcgaaca tccttcatac
    88141 ctaagatgac tgtgcagtca tattgccaga aatgtaaaaa gatttgctgg tgttctttag
    88201 aatattccgt taatatatct gttaatgcaa aaggggaaaa gtcgtttttt tgataaatgt
    88261 gctgatctag tcgaatcgca gggttcattc aaccggctcc tttgtataaa ttcatatttt
    88321 ctacaatatt atactctaaa atcaaagagt tgttttggaa aaaaggtttt ctgtaaaaat
    88381 ggacaagttg gttgacaaaa caaaagaaac taggaacaat gtagctatga gaagtaaagg
    88441 agaatgaata atggaattat cacaaggaac accgatttca atcaaattgc gtacaaaagt
    88501 aaaacagcaa ggggaagtgc aagattttta ttttgatttg aaagggcaaa tggtgacaat
    88561 tggcgatacg ctgtacatac gttataaaga gattcaggag gagacgggcg atgagattcc
    88621 tgtaacgatc aaattagttc cagatggtca tgtacagctg atccgtgcag gagaaatgcg
    88681 gatgcgtttg aaatttggct ataaagaacg gttagaaaca tcctatcgaa caccatacgg
    88741 tatgatccag attgctactt ttacgaaaga actgcatgtt agtttgaaag accgtcccac
    88801 aggagggaag gttcgaatcg attatgatct gttcatggga ccggaaaaaa tcggcgaata
    88861 ttatttgacc ttggatttta cagcgtaaat ataaaagaaa agtttgattt tttgtgtctg
    88921 attttgtatg ataaggagta gactgtgaaa ggacgtgtcc tgattggaaa ttagtgtatt
    88981 tgaaggtgca aacaaaagcg aattatcaat gatcgaagta gcacacgcga tcttagaaca
    89041 acgtggcgat gtcatggatt tttctgattt agctaaccag atccaaaact atcttgggaa
    89101 atcagacagt gaaattcgtg attcactcgc acaattttat actgacttga atattgacgg
    89161 cagctttatc tcattagggg ataatcgttg gggcttgcgt tcatggtatg ctatcgattc
    89221 gatcgatgaa gaagtaaacc atggaatgga cgaagaggat gaagatactc cacgtcgccg
    89281 taaacgcaag aaagtcaacg cctttatcaa tgatgatgaa gacgcgatcg attacaatga
    89341 cgatgaccca gaagatacag atctgacaga agaagatgac gatgatctgt ttgatgacga
    89401 tgatgatgaa gatgaagaaa tcgctgctta taattctgac ttgcaagaaa tcggagcaga
    89461 tgacccgaca gatgatgaag aagacttgcc aggaaatatc gaagaagatt taagtatcat
    89521 agacgaagac gatgatgatg aagattatga agaggaagaa ttttctgact ctgaaaaaga
    89581 ataaacatta ttttcttacg agaggttgtg acagagacga tcagcctatc agaataagga
    89641 ggtgtgacac gat
//
LOCUS       DAYYDS010000003        84918 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000003 DAYYDS010000000
VERSION     DAYYDS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.003"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1589)
                     /locus_tag="ACHA02_000198"
     CDS             complement(<1..1589)
                     /locus_tag="ACHA02_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8668583.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKI"
     gene            complement(1604..5125)
                     /gene="mfd"
                     /locus_tag="ACHA02_000199"
     CDS             complement(1604..5125)
                     /gene="mfd"
                     /locus_tag="ACHA02_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8668584.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(5142..5702)
                     /gene="pth"
                     /locus_tag="ACHA02_000200"
     CDS             complement(5142..5702)
                     /gene="pth"
                     /locus_tag="ACHA02_000200"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8668585.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            6055..7029
                     /locus_tag="ACHA02_000201"
     CDS             6055..7029
                     /locus_tag="ACHA02_000201"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8668586.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            7199..8599
                     /locus_tag="ACHA02_000202"
     CDS             7199..8599
                     /locus_tag="ACHA02_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8668587.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(8677..9399)
                     /locus_tag="ACHA02_000203"
     CDS             complement(8677..9399)
                     /locus_tag="ACHA02_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8668588.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(9478..9984)
                     /locus_tag="ACHA02_000204"
     CDS             complement(9478..9984)
                     /locus_tag="ACHA02_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8668589.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            10107..11906
                     /locus_tag="ACHA02_000205"
     CDS             10107..11906
                     /locus_tag="ACHA02_000205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8668590.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(11974..13548)
                     /locus_tag="ACHA02_000206"
     CDS             complement(11974..13548)
                     /locus_tag="ACHA02_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8668591.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(13803..14312)
                     /gene="mreD"
                     /locus_tag="ACHA02_000207"
     CDS             complement(13803..14312)
                     /gene="mreD"
                     /locus_tag="ACHA02_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8668592.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(14316..15170)
                     /gene="mreC"
                     /locus_tag="ACHA02_000208"
     CDS             complement(14316..15170)
                     /gene="mreC"
                     /locus_tag="ACHA02_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8668593.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(15325..15963)
                     /locus_tag="ACHA02_000209"
     CDS             complement(15325..15963)
                     /locus_tag="ACHA02_000209"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8668594.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(15964..16614)
                     /gene="rpe"
                     /locus_tag="ACHA02_000210"
     CDS             complement(15964..16614)
                     /gene="rpe"
                     /locus_tag="ACHA02_000210"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8668595.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(16627..17526)
                     /gene="rsgA"
                     /locus_tag="ACHA02_000211"
     CDS             complement(16627..17526)
                     /gene="rsgA"
                     /locus_tag="ACHA02_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8668596.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(17691..19760)
                     /gene="pknB"
                     /locus_tag="ACHA02_000212"
     CDS             complement(17691..19760)
                     /gene="pknB"
                     /locus_tag="ACHA02_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8668597.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(19757..20497)
                     /locus_tag="ACHA02_000213"
     CDS             complement(19757..20497)
                     /locus_tag="ACHA02_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8668598.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(20510..21868)
                     /gene="rsmB"
                     /locus_tag="ACHA02_000214"
     CDS             complement(20510..21868)
                     /gene="rsmB"
                     /locus_tag="ACHA02_000214"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8668599.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(21868..22806)
                     /gene="fmt"
                     /locus_tag="ACHA02_000215"
     CDS             complement(21868..22806)
                     /gene="fmt"
                     /locus_tag="ACHA02_000215"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8668600.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(22799..23290)
                     /gene="def"
                     /locus_tag="ACHA02_000216"
     CDS             complement(22799..23290)
                     /gene="def"
                     /locus_tag="ACHA02_000216"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8668601.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(23305..25713)
                     /gene="priA"
                     /locus_tag="ACHA02_000217"
     CDS             complement(23305..25713)
                     /gene="priA"
                     /locus_tag="ACHA02_000217"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8668602.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(25966..26271)
                     /gene="rpoZ"
                     /locus_tag="ACHA02_000218"
     CDS             complement(25966..26271)
                     /gene="rpoZ"
                     /locus_tag="ACHA02_000218"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8668603.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(26276..26890)
                     /gene="gmk"
                     /locus_tag="ACHA02_000219"
     CDS             complement(26276..26890)
                     /gene="gmk"
                     /locus_tag="ACHA02_000219"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8668604.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(27377..27661)
                     /locus_tag="ACHA02_000220"
     CDS             complement(27377..27661)
                     /locus_tag="ACHA02_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668605.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(27679..28569)
                     /locus_tag="ACHA02_000221"
     CDS             complement(27679..28569)
                     /locus_tag="ACHA02_000221"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8668606.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(28589..28807)
                     /locus_tag="ACHA02_000222"
     CDS             complement(28589..28807)
                     /locus_tag="ACHA02_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8668607.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(28777..30477)
                     /locus_tag="ACHA02_000223"
     CDS             complement(28777..30477)
                     /locus_tag="ACHA02_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8668608.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            30744..31517
                     /locus_tag="ACHA02_000224"
     CDS             30744..31517
                     /locus_tag="ACHA02_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668609.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(31598..32167)
                     /gene="thiT"
                     /locus_tag="ACHA02_000225"
     CDS             complement(31598..32167)
                     /gene="thiT"
                     /locus_tag="ACHA02_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8668610.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(32233..32335)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(32341..33312)
                     /locus_tag="ACHA02_000226"
     CDS             complement(32341..33312)
                     /locus_tag="ACHA02_000226"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8668611.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(33406..34779)
                     /gene="glmU"
                     /locus_tag="ACHA02_000227"
     CDS             complement(33406..34779)
                     /gene="glmU"
                     /locus_tag="ACHA02_000227"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8668612.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(35282..36100)
                     /gene="purR"
                     /locus_tag="ACHA02_000228"
     CDS             complement(35282..36100)
                     /gene="purR"
                     /locus_tag="ACHA02_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8668613.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(36267..37091)
                     /locus_tag="ACHA02_000229"
     CDS             complement(36267..37091)
                     /locus_tag="ACHA02_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8668614.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(37064..37744)
                     /locus_tag="ACHA02_000230"
     CDS             complement(37064..37744)
                     /locus_tag="ACHA02_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8668615.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(37767..38705)
                     /locus_tag="ACHA02_000231"
     CDS             complement(37767..38705)
                     /locus_tag="ACHA02_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8668616.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(38950..39801)
                     /gene="ispE"
                     /locus_tag="ACHA02_000232"
     CDS             complement(38950..39801)
                     /gene="ispE"
                     /locus_tag="ACHA02_000232"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8668617.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(39955..42528)
                     /gene="mprF"
                     /locus_tag="ACHA02_000233"
     CDS             complement(39955..42528)
                     /gene="mprF"
                     /locus_tag="ACHA02_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8668618.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            42669..43475
                     /gene="proC"
                     /locus_tag="ACHA02_000234"
     CDS             42669..43475
                     /gene="proC"
                     /locus_tag="ACHA02_000234"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8668619.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(43554..45110)
                     /gene="rny"
                     /locus_tag="ACHA02_000235"
     CDS             complement(43554..45110)
                     /gene="rny"
                     /locus_tag="ACHA02_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8668620.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(45521..46570)
                     /gene="recA"
                     /locus_tag="ACHA02_000236"
     CDS             complement(45521..46570)
                     /gene="recA"
                     /locus_tag="ACHA02_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8668621.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(46679..47920)
                     /locus_tag="ACHA02_000237"
     CDS             complement(46679..47920)
                     /locus_tag="ACHA02_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8668622.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(48160..48738)
                     /gene="pgsA"
                     /locus_tag="ACHA02_000238"
     CDS             complement(48160..48738)
                     /gene="pgsA"
                     /locus_tag="ACHA02_000238"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8668623.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(48947..49870)
                     /locus_tag="ACHA02_000239"
     CDS             complement(48947..49870)
                     /locus_tag="ACHA02_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8668624.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(50609..51895)
                     /locus_tag="ACHA02_000240"
     CDS             complement(50609..51895)
                     /locus_tag="ACHA02_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8668625.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(51888..53156)
                     /locus_tag="ACHA02_000241"
     CDS             complement(51888..53156)
                     /locus_tag="ACHA02_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8668626.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            53408..53869
                     /gene="mscL"
                     /locus_tag="ACHA02_000242"
     CDS             53408..53869
                     /gene="mscL"
                     /locus_tag="ACHA02_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8668627.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(53929..54375)
                     /locus_tag="ACHA02_000243"
     CDS             complement(53929..54375)
                     /locus_tag="ACHA02_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8668628.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(54468..54713)
                     /locus_tag="ACHA02_000244"
     CDS             complement(54468..54713)
                     /locus_tag="ACHA02_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8668629.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(54828..55406)
                     /locus_tag="ACHA02_000245"
     CDS             complement(54828..55406)
                     /locus_tag="ACHA02_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8668630.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(55483..56013)
                     /locus_tag="ACHA02_000246"
     CDS             complement(55483..56013)
                     /locus_tag="ACHA02_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8668631.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(56017..56877)
                     /gene="rlmB"
                     /locus_tag="ACHA02_000247"
     CDS             complement(56017..56877)
                     /gene="rlmB"
                     /locus_tag="ACHA02_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8668632.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(56870..57265)
                     /locus_tag="ACHA02_000248"
     CDS             complement(56870..57265)
                     /locus_tag="ACHA02_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8668633.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(57262..58674)
                     /gene="cysS"
                     /locus_tag="ACHA02_000249"
     CDS             complement(57262..58674)
                     /gene="cysS"
                     /locus_tag="ACHA02_000249"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8668634.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(58671..59207)
                     /gene="epsC"
                     /locus_tag="ACHA02_000250"
     CDS             complement(58671..59207)
                     /gene="epsC"
                     /locus_tag="ACHA02_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8668635.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(59293..59532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(59529..60989)
                     /gene="gltX"
                     /locus_tag="ACHA02_000251"
     CDS             complement(59529..60989)
                     /gene="gltX"
                     /locus_tag="ACHA02_000251"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8668636.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(61159..62316)
                     /locus_tag="ACHA02_000252"
     CDS             complement(61159..62316)
                     /locus_tag="ACHA02_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8668637.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(62350..63720)
                     /gene="radA"
                     /locus_tag="ACHA02_000253"
     CDS             complement(62350..63720)
                     /gene="radA"
                     /locus_tag="ACHA02_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8668638.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(63783..64310)
                     /locus_tag="ACHA02_000254"
     CDS             complement(63783..64310)
                     /locus_tag="ACHA02_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8668639.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            64540..65346
                     /gene="proB"
                     /locus_tag="ACHA02_000255"
     CDS             64540..65346
                     /gene="proB"
                     /locus_tag="ACHA02_000255"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8668640.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            65343..66590
                     /locus_tag="ACHA02_000256"
     CDS             65343..66590
                     /locus_tag="ACHA02_000256"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8668641.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            66666..67010
                     /locus_tag="ACHA02_000257"
     CDS             66666..67010
                     /locus_tag="ACHA02_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668642.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(67127..67453)
                     /locus_tag="ACHA02_000258"
     CDS             complement(67127..67453)
                     /locus_tag="ACHA02_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668643.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(68068..69270)
                     /locus_tag="ACHA02_000259"
     CDS             complement(68068..69270)
                     /locus_tag="ACHA02_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8668644.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            69387..69902
                     /locus_tag="ACHA02_000260"
     CDS             69387..69902
                     /locus_tag="ACHA02_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8668645.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(70101..70547)
                     /locus_tag="ACHA02_000261"
     CDS             complement(70101..70547)
                     /locus_tag="ACHA02_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8668646.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(70594..72072)
                     /gene="msr(C)"
                     /locus_tag="ACHA02_000262"
     CDS             complement(70594..72072)
                     /gene="msr(C)"
                     /locus_tag="ACHA02_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8668647.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(72426..73055)
                     /locus_tag="ACHA02_000263"
     CDS             complement(72426..73055)
                     /locus_tag="ACHA02_000263"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8668648.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(73039..73503)
                     /locus_tag="ACHA02_000264"
     CDS             complement(73039..73503)
                     /locus_tag="ACHA02_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8668649.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(73990..75258)
                     /locus_tag="ACHA02_000265"
     CDS             complement(73990..75258)
                     /locus_tag="ACHA02_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8668650.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(75489..76226)
                     /locus_tag="ACHA02_000266"
     CDS             complement(75489..76226)
                     /locus_tag="ACHA02_000266"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8668651.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(76293..76862)
                     /locus_tag="ACHA02_000267"
     CDS             complement(76293..76862)
                     /locus_tag="ACHA02_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8668652.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            76990..77862
                     /locus_tag="ACHA02_000268"
     CDS             76990..77862
                     /locus_tag="ACHA02_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8668653.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            78204..79076
                     /locus_tag="ACHA02_000269"
     CDS             78204..79076
                     /locus_tag="ACHA02_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8668654.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            79073..81031
                     /gene="nagE"
                     /locus_tag="ACHA02_000270"
     CDS             79073..81031
                     /gene="nagE"
                     /locus_tag="ACHA02_000270"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8668655.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(81363..82214)
                     /locus_tag="ACHA02_000271"
     CDS             complement(81363..82214)
                     /locus_tag="ACHA02_000271"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8668656.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            82337..82804
                     /locus_tag="ACHA02_000272"
     CDS             82337..82804
                     /locus_tag="ACHA02_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8668657.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(83067..83549)
                     /locus_tag="ACHA02_000273"
     CDS             complement(83067..83549)
                     /locus_tag="ACHA02_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8668658.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(83649..84512)
                     /locus_tag="ACHA02_000274"
     CDS             complement(83649..84512)
                     /locus_tag="ACHA02_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8668659.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        23985 a        18210 c        15313 g        27410 t
ORIGIN      
        1 ttaatttttt tatttaatcg caggattttc ttaccaaaag gcaacattag ccattctcgt
       61 actgtaaaca gacgaagacg aatcactgca taaatgaaca cagaagcccc gatcccaact
      121 cctatcacgc tgacaaaaaa agcagtggaa cgatgagtta caggaccaaa tactatattc
      181 aatacaccat aatacagcag cagtactact gtcatacaaa gaaggctgaa catcaacttc
      241 cgtccaaaat gccgttcttt ccaaaaagca tttagctctt ttgtttcaat tcgaacaaaa
      301 taccaaagcg tcacacctaa tcctaaaagg gtagacaggc ttgcacctgc tgttcctaat
      361 aaaccggtca gaaaagaagt agtcaatcct tttaccaata gtccccatcc tgcgcctttt
      421 aaagaagggc gaaacgagtt cttgctttga gcaatacttt gataagcttg gatgactgca
      481 gtgaaagcaa tggaaaaaac aaataataca agcgctgcat ttcccgcata atctttaaac
      541 aacgcataat tgatataagg gagcagcaag gctaatccca aagacgcagc taatgctaaa
      601 gcggtcgtca atctaagata gattttggct gtctgtaaaa actgacgata gatccggttg
      661 gtcaaatgac gagtcaacgc aggcaggaat gtcgcactta atgcagtggc aatcactaaa
      721 cctaattgta ctaaaggctg acctcgatca tagacccctt ttgctatctt cgcaccgtat
      781 tctgacagtc cgtaaacttc caaagcattt tttacaaaga aagagtcgat cagctggaaa
      841 aagataagca atccactata aatcgaaacc agtccgcctt ctaccagaaa acgccggatc
      901 aagggctttg acggctgttt gaacaatgga aaccgtcgga agcttaacgc tcctccatga
      961 atcttttgtt catagtagtg aaggatccca tatgcgcata aaccgccaaa aacagctcca
     1021 ctcattgctg ccgtccctgt ttggtacacc gtccatccaa gctttctaaa agcaaacgca
     1081 ctacagacaa tcacaaaaac acgaacgaac tgctcaacta cttgagaaac agcagttggt
     1141 tccatcaaga atcttccttg gaaattccct cgataaaaag atagaccagg catcagcaaa
     1201 aaggtaaagg aaactacttg gatcagcggt ttcaattgta tgtctcccat catccaagca
     1261 atccagctgc taaatgcaaa gacaaacaac cacaaaaaga cacctgtcca aaatactaag
     1321 ggataaagat tttgcaaagc ttgctttcgc tcgaaaggat tctttctttc tgcaacgtac
     1381 tttgatatga actgcggaag accggataaa gctaaagtca ttgctatccc ataaattgga
     1441 taaacttgct gataaacata aaaaccttcg tcgccgacta agttttggag gggaattcgg
     1501 taaagggcac ttaatacttt tgctatgaaa gaagcaatcg tcaatacaaa tgctccttgc
     1561 atgacgcgcc gcatttcttt atgatccaaa tcattgctcc ttcctacgct gacagatatt
     1621 tttgctcacg caacgctttg acaaactttt ggatttcctg aagccatact gcttcttcca
     1681 tttggttggg gatcgtcaag cgtatcacca tctgttcttt ttcaacgcct agactggctt
     1741 ttagttttgt cgctgtcagt gcttcgaata actgttccac actatagcgt tttgttccaa
     1801 ctttacttag agtaaagact atatgcgcct cctgtttcct tatcgtttct aggagcgctc
     1861 gatcgccatc cattttgatc tgcccaattg tcaagagatg tgctacttct tctggatatt
     1921 ctccaaaacg gtcgagcaga tcatcttcta gttcatctag catttcttgc gaatcaagtt
     1981 cacgaattcg tttatagatc tcgattttct gccgttgatc tgccacgtat gtttcaggta
     2041 gatacgcatc gacaccgagg tcgatctcaa cagtggtctt ctccacttga ctattctttc
     2101 cttgtttacg agaaacagct tcgcttagca tttgagaata catatcgaaa ccgactgcat
     2161 caataaatcc atgctgctga gcacctaaga gattacctgc accgcgaatg gaaagatccc
     2221 gcatcgcaat cttgaatcca gatcccagtt ctgtaaaatc tttgattgcc tgaagacgtt
     2281 tttcactgac ttcgttaagg atcttctgct gctcatacat aaagtaagca tatgcgacac
     2341 ggctgcttct tcctacccgc cctcgcaatt ggtataacgt ggataatccc atataatcag
     2401 cgttctcaac gaacaacgta tttgcgttcg ggatatctac tcctgtttca atgatggttg
     2461 tagtgacaag aacatcatat tgaccttcaa taaattcaaa aagcgtattt tctaattgcg
     2521 cctctgtcat ttgcccatga gcataaccga tccgtgcttc cggaacgagt tcttggattt
     2581 cttctacttt ttgttcgatc gtttctaccc gattatataa atagaatact tgtccacctc
     2641 gtcccatttc tcgatggatt gcttcgcgaa tagccccagg atttttttcc ataacatacg
     2701 tttggatcgg ataacgattc gcaggcggtg tttcgattac tgaaagatcc cgcacgccta
     2761 acatcgacat atgcaaagtt ctcggaatcg gtgttgcagt cagagtcaaa acatccactt
     2821 gtgcacgtaa ctgtttcaat cgttccttat gttttacacc aaaacgttgt tcttcgtcaa
     2881 tgatcagtag acccaaatcg ctaaattcaa tatcttttga caaaattcgg tgggttccta
     2941 caacaatatc aacttgacct gtacgcagtt gttcgatggt ttctttttgt tgctttcttg
     3001 ttctgaaacg acttaatagc ccgatattta cggggaaacc ttcaaatctt tccagcatcg
     3061 tttcatagtg ctgctgtgcc aagatcgttg ttggaactaa aaaggctact tgtttgcttt
     3121 cctttatcgc tttaaaggcc gctcgaagcg ctacttctgt tttcccataa ccaacatcac
     3181 ctacgagtag tcgatccatt ggtttttctt tttccatatc ccgtttgatc tccgctgcac
     3241 ttctcaactg gtcatcagtt tcagagtagg ggaaggcatt ttcgaattct ttttggtagg
     3301 cgtcatcagg tccaaaagca tatccttttt ctgactctct tgcggcatat aatttgatta
     3361 gatcgtcagc gatatcctcg atttttgaag agacttttcg cttggttttc gtccattcac
     3421 ttccgcctag cttattgatg cgaggcgctt tggcttcaga ggcgacatat ttttggatca
     3481 gattcagttg agtaacagga ataaacagtt tgtcgtcgtt ttgatacaaa atagtcatat
     3541 agtcttggtg aacgccatct acttctagcg tttccatccc aatgtatttc ccgataccat
     3601 gattggcatg gacaacgtaa tctcctgctt ttaactcatt atagcttttc aatcgctctg
     3661 cgtttgttac tgtttgacgt ctcgtttgtt ttttcgtcgt tttttggaaa atttcttttt
     3721 ctgtgatagc tactattttt tcttgaggaa gttcaaaacc agcctgcaaa gctccttcaa
     3781 ctaattgtac ctgtcctctg atcaatgcat ttttttctgt ttctactact aatatgtctt
     3841 catctcgaag catttgttct gctttgcgta ttctgtcctt tgtcggaata aagatcacaa
     3901 cagtctgttt ttgcttgcgc caacggtcca tttctgtttt cagcaatggc atttgtccga
     3961 agaactgttg catcgtccga tattgaaaat tatggattgc ttggaaacga agatttccca
     4021 tccctttttg gaagagtgag aaaaaggttg tggtgaactt ttccttctgc acgatttttt
     4081 gtatatctgc tgaaaatgtc tgctcagaaa acacccgcaa ttcgctgatt ttttgagtct
     4141 gccattctgc cgcttccctt tcgatttccc gatttgtttc catgattcgc tgataatcat
     4201 caacgactag taaactattt gaagggaaat aatccaagat cgtcacagat tcttggtaaa
     4261 gaagatctga gtagaatttt gcttcttctg tcgggacacc tgcttgccag cttgaagtca
     4321 gctgaccgaa gtaatcttgc aaaaactcgc ggtctgcttc ttctgtggtc gcagcaagcc
     4381 gtttttctag cattttttgc atttgctcta ttccatgttc tagatcctct ggtccgtaga
     4441 cttgttcact cgttggggaa acccagattt cttccgtttt ttcgattgag cgttgtgttt
     4501 ctgcttcaaa ataccgcata gaatctatct ctacatcaaa tagttctaca cgaacaggat
     4561 aggttgtcgt taacgggtaa atatcgataa tgcttcctcg aatactgaat tctcccggtt
     4621 ttgcgaccat ctcttctcgt acatagccca tcaaagtcaa ttggcgagga agattttcca
     4681 cgtctatctc atcaccaata tgccaattca attgcgcctg tagccaagtt ttcttattcg
     4741 gcagtcgttt gtgcaaagat gcgactggca acaaatagat acctgctttt ttttcttgaa
     4801 gtgcgttaag cgttgcaact ctctcgcctc gcgcttctgg agaagaaaag gccatttcag
     4861 cagaaagcac ttcatccact ggaaataaat ggatctgatc ttgcggaatc acatgttgca
     4921 gatcttctgc taactgattc gcataataca aatttggcat cagaataatc atgtttttat
     4981 ttgttttctt aaacgctcga gcaaatagaa gtgttttcgc tgagcccgct aatcctgtga
     5041 ttagttgtct aggcattttt tcttctaaat tgctgaccca ttcttttact tgcggatctt
     5101 ttccgactaa ttgaataata tccattcctt ttcctccttc ctcaattgaa tcgattcatc
     5161 gcatccacga acgaatgccc ttctatgaga tattcgactg ctttaacaga ttgacacatg
     5221 ctttcttcta tcaaaggctg attttctttg ctgaaaggac taagcacatg ctggactact
     5281 gtttttttcc cttctggcct gccgattccc accttgattc gatcaaagac attcgtattc
     5341 aaatgggaaa taatactttt gataccgtta tgcccgccgg cacttccctt ttgacggagc
     5401 cggatttttc ccactgccag atccaaatca tcgtagatta cgactaattc ctctggataa
     5461 atcccaaaat aagtcattag tgggccgact gcacgaccag attcattcat gaatgtttgt
     5521 ggtttgacta aaaggatctt ttctccatta tgaaaaaatt cggcaacttc tgcttcgaac
     5581 ggattttttt tgaaagtagc ttgatgctcg cgtgcaattt tatccacgac cataaagccg
     5641 atattgtgtt ttgtatattg gtatttggta cctgggtttc ctaacccgac gatcattttc
     5701 atttttgttt tccacctaat tctaaaaagc agcacaaaag gctggactgc taattagcgg
     5761 tccagtccat cgagcttttt tcttttttac acttgtcaat gcatagtata acacaaaaca
     5821 aggcgtttcg gctaaaagag aatcgttaat aaatgataaa gaaattaaaa atttcttcaa
     5881 agtttaatga aataattgat atataaaaat agtgaaataa cgcacatgta agcttttaca
     5941 tataatacaa ccttgttatc tgttatattt tatagccaaa tagcgtgaca tggggcttga
     6001 gaaatgttag cataagcatg ggaaaaaaca aatcaatgga aggattgata ctaaatgact
     6061 gcagctgcag aaaaaaaaga tcatcaaaaa gttattcttg ttggagacgg cgccgtaggt
     6121 tctagctatg cctttgctct agttactcaa aatatcgctc aagaagtagg tattgtggat
     6181 atcaatactg ctaaaactga aggagacgca attgacttgt ctcacgcttt ggcatttact
     6241 tcacctaaaa aaatctattc tgctacttac gcagacgcac atgatgctga cttagttgtt
     6301 attacagctg gcgcaccaca aaaaccaggt gaaactcgtt tagacttagt aaacaaaaac
     6361 ctaaaaatca accgcgatgt tgtcacacaa atcgttgaat caggatttaa cggtatcttt
     6421 ttagttgccg ctaacccagt agatattttg acttactcaa cttggaaatt ctcaggtttc
     6481 cctaaagaac gcgtaatcgg ttcaggtacc tctcttgact cagctcgttt ccgtcaagca
     6541 cttgcggaat tagttgacgt tgatgcacgt aacgtccatg cttacatctt aggtgaacac
     6601 ggagattcag aattcccagt ttggtcacat gcaaatgttg ccggcttgca aatctatgaa
     6661 tgggtaaaaa acaacccaga aatcgatgaa gaagctatgg ttaacctatt ctttggcgta
     6721 cgtgacgctg cttacacaat tatcgagaaa aaaggcgcta ctttctatgg tatcgcagta
     6781 gcattagcac gtatcactcg tgccatctta gatgatgaaa atgctgttct tcctttatca
     6841 gtattcatga atggcgaata tggattgaac gacatctaca ttggtgcacc tgctgttatc
     6901 aaccgtcaag gtatccaaaa agtaatcgaa attcctttaa gtgacagcga gcaagaccgt
     6961 atggctgctt ctgctaagca attaaaagaa attctagatg aagcttttgc taaactagac
     7021 gctgaataat ctttgaacat gacggaaaac atctattcgg tgttttccgt ttttttattt
     7081 tatcaccctt atggtttcta aattttttca taaagtagag ataaccttcg tcttttcctc
     7141 taaattatgc tatcatgaga ccgttccacc ctaaactttt acttaaggag ttgtcgatat
     7201 gacaagatct tcgcatcgaa aaaaatcgaa acatgttgct ttgctttcag ttcttggtgt
     7261 tctcttagtg ctgatcagct tttataccta tcgcagtaca tactacacaa agcatttttt
     7321 gccgaataca gaaataaatg gaataaatgt cagcaatcta acagtcgaaa aagcaaatga
     7381 aaaattaaaa gaagcttatt ctgataaaaa actggctatt aaagaagatg gaaaagtctg
     7441 gcaggaagta ccaaaatccg agctcggcta caaagatgat ttcacttcgg agctttctcc
     7501 gatcctaaat gaacaaaacg gatggacttg gggaatgact tatgtctcag cggcagaaaa
     7561 acaagaaata gatcctgtca gtcaaaacca ccaaaaacta gacactaccg ttcagacatt
     7621 gacaactaag ctgactgatt tgaacaaaga ccggacacct actactgatg caacgatcga
     7681 aaaagcaggt gactctttcg ttatcaagcc tgaagtcaat ggaaatacga tcgacgtcga
     7741 tgctgcggtc aaacaattaa aaagtgcggt aaactctggt aaagatacaa ttgaattgac
     7801 tgaattcaaa gaaaaaccaa aagtaacatc tgaagacagc tcactgaaag aacaactcgc
     7861 atccatgaat gctatcgcaa atgtaaaagc tacttacagt ataaacggtg agactttcca
     7921 gatcccatca agtgatatta tgagttggct gacctacaat gatggaaaag tcgatttaga
     7981 tacagaacaa gtacgtcaat atgtaacaga tttaggcaca aagtataaca caagtacgaa
     8041 tgacacaaaa tttaaaagta caaaaagagg agaagttacg gtcccagttg gcacttacag
     8101 ttggacgatc caaacagaca gtgagacaga agcattgaaa aaagcgattc tggcaggcca
     8161 agattttact cgttcaccca ttgtgcaagg tggcacaacc gctgatcatc cattgatcga
     8221 agatacgtat atcgaagttg atctggagaa ccagcatatg tggtattaca aagacggaaa
     8281 agtggccctt gaaacagata tcgtttccgg aaagccaact acacctacac ctgctggagt
     8341 cttctatgtt tggaataaag aagaagatgc aactttaaaa ggaacaaatg acgatggaac
     8401 accttacgag agtccggtaa actactggat gccgattgac tggacaggtg ttggtatcca
     8461 cgattcagac tggcaacctg aatacggtgg agacttatgg aaaacccgcg gttcacacgg
     8521 ctgcatcaac accccaccaa gtgtaatgaa ggaattattc gggatggtcg aaaaaggaac
     8581 gcctgtattg gtcttctaac aaaaataata tataaaaaga aagcacccgt acgattggta
     8641 caggtgcttt ctttttatta tctaacaatg tgctacttac atttcagcag cagtaaagac
     8701 ttctgaaaca tcatcatcgt cttctagctt atctactaga cgttccaatt gctctttttg
     8761 ttcctcattt aaatcaatcg tcgtttgtgg aatcatcgtc aattctgctt gcgccaaagt
     8821 gaactctttt tccaactcat ctcgtacagc agcaaaatct tctggtgcag tataaatctc
     8881 gaatacttca ggagaagttt gcagatcttc tgcccccgcc tctaatacat cctcaaacat
     8941 cgtctcttca tcaacagtca acccatcgcg ttcaatagcg atatatcctt tgcggtcaaa
     9001 caagtaactg actgaacccg tttctcccag tgatccaccg tttcgagtaa aagctactcg
     9061 gacatttgtc gctgtccgat tacggttatc tgtcaatgca tggactaaga ttcctactcc
     9121 acccggacca tatccttcat acgtgatttc atcgtaattc gtttcatcta cggcactcgt
     9181 agccttttta atcgcccgtt ccacattgtc gttaggcata tttgcagctt ttgctttgtc
     9241 cacaacaagc cgcaaagctg gattcataga tggatcagga ccgcctgctt tagctgccat
     9301 ataaatttca cgagaaagtt tctgaaagac tttccctcgt tttgcatctt gggcattttt
     9361 tcttccttgg atattatgcc atttactatg tcctgacatg atttttcctc ccttgttctt
     9421 ttgagaatac acttctattc tagtactatt tcacaaaaaa acaaagccac agccaatcta
     9481 taaacgcttc ttgaatgccg caaaaagcac acataatatc acagcagtca gagcaccgat
     9541 cgaatccaac atgacatctt ggaacatagg tgttcgtcca ccggtcatca tctgatggaa
     9601 ttcgtccaaa cctgcgtacc ctgttgctga aagccaagca aggatcgctg tcagccacca
     9661 aatttttagc ttggggaaaa cacctaaaaa caagctgcct cccaaaataa aataggtacc
     9721 gaaatgtgcg cccttcctta aaaagaactc taaaaatttg gcgtagccca gatggttgat
     9781 actcaccata cttccaccat aattaaaacg tatacccgct aatgtgtctt taaatggctg
     9841 attaggcagc cagctttcta atcgcgaaac ttgtgattgc tgtccgtaag tttgagaaga
     9901 actgtaaaat aagattataa cgactatcag tgcgattact aaataaatat ttccgttttt
     9961 catttgtttt tttgaagaat tcataagtca cctctgtatt ttatcttaga ctttttttga
    10021 agaaacaaca atcattttca tagagacttc ttccataaaa aagtgctata cttaattttg
    10081 tattacaata tgaagggagt tttttcatga gttattcatt aaattggaat ctagattcta
    10141 ttttttccgg aggcagtcat tctgatgctt tgaaccaacg gatgaagcaa ttggaagacc
    10201 aaatgaacga gtattatcac cgcgtaacaa aatggtcacc tagttctgac aatgcagagc
    10261 agttaaacgc tattcttcaa ttgcaagaaa cgattacgaa tggttttacc caatgcagca
    10321 gctatattac tgcattgctt tctgccaatg tcaatgattc agatgcaaag gtcttatcag
    10381 gcaagcttta cgcaatgctg cctcgtctgc aatcagccga aactgtattg tcaaagaaat
    10441 ttgcagaaat atctgacaat gattggaatc agatgctttc tcaatcttcg tttgaaacaa
    10501 ttgcttttcg tttaaacgag attcgtcgtg atggctcaca attattatca gaagctgaag
    10561 aaaacattat aaatacgctt tctttagatg gtctaaatgc ttggagcagt cattatgata
    10621 cgattgtagc cagcatttcg attcccttcg aacaagatgg acaagtagtc gaattatctg
    10681 ctggacaagc cttcaataag atgatgggcg atccagatcc aaaagtcaga gaaacacttt
    10741 tcgcagcatg ggaagaagcc tggaaagaaa aggcacccct cttcacggat actttgaatc
    10801 atttagatgg ttttagactt tctgactatg aacttcatgg tgtcacagat tttctacaaa
    10861 aaccattaga gtacaatcgg ttgaagaaag aaacattgtc tgtcatgtgg gatacaatcc
    10921 agaaaaacaa acaacccctt gttaattact tgactagaaa agctaatctt ttcggtaaag
    10981 aaaaaatgga atggcaagat caagatgcac ctattatttt aggcgatttg aaagaaaaaa
    11041 cctttagttt tgacgaagca gcagcattta ttatcgaaaa ctttaacaaa ttcagtccaa
    11101 aaatggctaa atttgcccaa tctgcttttg aaaagagctg gatcgaagca gaagatcgtc
    11161 ctgggaaacg tcctggtggt tattgtactg agttaccaga aacaaaagaa tcaaggatct
    11221 ttatgaccta cagtaattct gttaatgagg ttgcgacact tgctcatgaa ctaggacatg
    11281 ctttccatag cagcacgatg tgggatttac cttctttaaa tcgcgaatat gccatgaatg
    11341 tcgcagaaac agcaagtaca ttcgcagaac tgatcgtagc cgatgctaca ttaaaagtcg
    11401 caaaaacaaa agaagaaaaa atcaatttgc tagatacaaa actgcaaaat gctttagcta
    11461 tgttcatgaa tatccattcc cgattcattt ttgaaaatcg attctatgaa gcacggcaag
    11521 aaggattagt ctcagaagat aagatcacag agatgatggt tgaggcgcaa aaagaaggct
    11581 atcaaggagc tttagctact taccatccat atttctgggc tgcaaaattg catttcttta
    11641 ttgatgacgt accgttttat aacttcccat acacatttgg ctacctcttt agtttgggca
    11701 tctatgctta cgccaaccaa aagggagcaa attttgaaga agaatatatc catttactac
    11761 gcgatactgc ctcaatgacg acggaagatc ttgcgaagaa acatttgaat gtcgatctta
    11821 cccaaccaga tttttggcaa gcaggtatcg acatggtgac tgaagatatc caaacgttca
    11881 tggaattgac agaagaattt atctgatttc tattttatca caataaaaaa agagttagtc
    11941 cagaatgggc taactctttt tgattaatca ttcttacatg ctgacagcaa agtcaggggc
    12001 aaaccattga actgaaccaa cttttacgct ttcgcctggt tgaggagcat ggatatattg
    12061 tcctccgcct aaagcaatcg ctacgtggta agttccgcct tgtgaccccc agaataacaa
    12121 gtctccagct tttgcttgag aaactgaaat ttttgttcca gctgattctt gaggaactgt
    12181 ccaaccaccg atgtcacgac cagttacttg catgtaaaca tagcgtgtga accctgagca
    12241 gtcaaagcca cttggatctt taccgcccca aacatatgga gtaccaatat atttgtaagc
    12301 ttctgctacg attgctgcac cattcacact tccagaagga gctggagcag gtgttacagg
    12361 ttttggttgt tcaggtgttg gtgttggcgt tggagccgga gtactacttc ctgtgccgtt
    12421 tcctgtatca acactttgat ctgttgatgg tgttgtaggc gcaggtgttg ttgattcttc
    12481 tgttgttgaa gattcaggca ctgttgttga ttcttctgtt gctgaagatt caggcactgt
    12541 tgttgattct tctgttgctg aagattcagg cgctgttgtt gattcttctg ttgctgaaga
    12601 ttcaggcgct gttgattctt ctgttgaaga ttcaggtgtt gttgattctt ctgttgctga
    12661 agattgtgta gctgatgatg attcttcagt cattgaagat tgtgtagctg atgattcttc
    12721 tgttgctgag ctttgtgcag tagatgaagc ttgagtagct tgtgcttcag cctctgcttg
    12781 ttcgcgtgct tctttttcag ctttttcttg tgcagcttgt tgacgagctt gttctgctag
    12841 acgttgttgt tcacgaatac gagcttgctc tgcttcagct tctgcttttt gacggttcaa
    12901 gtcagctttc ttgtcttcag cagttgcttg ttcagcagct aatgaagttt tcagtacgtt
    12961 caaatcagct tgtttagata aaagatcgcc tttttgagat tccaaagcag cttggttttc
    13021 agctaattct tttagtttag catcgttttc agcttttttg tcttctacag cttttttatc
    13081 ttgtttttgt tgttccatca agtcgttgtt tgcttttacc atcgttgtca ttgcttgaac
    13141 gcgtccaata gcatctgcta atgaatcagc attcaataca gcatcgatgt agtttgagct
    13201 tgtgttgctt acttgtgctt cgcgtgcttg tttttggatt gtatcttcac gtttttcaat
    13261 acgttcttgt aaatctgcaa tgtcttttac taattgtgca gattcttgac gcaatgtatc
    13321 ttgttttgct aataggtctt gtgcttgtgt gttgatttca gaaacttggc tttctaaggc
    13381 atcgatttgt gattgtgcat ctgcttgttg atttttcaag tctgcaatct tttgatcttg
    13441 ctgttgtatc tgagaatcaa aatcgtccgc cgcggcagcg atcggtgatg ctacggcagt
    13501 caaggtcatt gaacatacca ttactgctga tattaaactc tttttcactc ttcattcctc
    13561 cgactggctt aatttaataa atatctaaat atatttaagt ctttcttaac gatataaaaa
    13621 ttgtaccata gcagtgtgtc aacgatataa tagttatgtt acaaaaatgt ttcaaaacaa
    13681 cttttaaaaa tgacaaaact gttcgtttca gaaaatgatc atgaaaaagc cgcgttaagt
    13741 ctagcatttc ttaagataac aacgattctt tttcgctttc gacctaaaaa taaaaaaaga
    13801 aattattcat cactgaataa tttcttaaag ggaaaaatga ataatacaaa tataatcatg
    13861 ttcaacagca gtgtaggtcc taaaaacctt gtaataaaat acgtgttatt tactactgct
    13921 aatttaaaaa tcaactggac caccatcgta aacaattcat aacccgtgac aaagatgatc
    13981 atactgaaaa attcagtaaa gatgttgaca tggataacgt ctttcattac atacatcaac
    14041 catacaatta aagggagagc tactgcatag ataccgataa cgccaatata gtaagcgtca
    14101 tagatagccc cgagaacaat cgtggtaatc agtaaataac gtttttcaaa cgccatactg
    14161 caacataata atgccaatat cagaaagtgg gcatttgaca tataggtgcc tttcgaccac
    14221 tcccctagca ttcttgttaa atgaccatct atcaacatca aaaagaacaa aacgattggc
    14281 agatagtact tcattgtttc tttcttcaac atggactaat cctccgctag ccgttggaca
    14341 atcgtcacaa ctgacacgtc atacatttct gcataaggtt tcacataaac ttcccgatcc
    14401 agcccataac tatctggttt cgttttaata accgtaccga tcggcaagtt agctggagaa
    14461 ttccctccaa gaccggatgt ctggacaaca tccccttctt tgatatccgt atcaccagtt
    14521 aattgggtca ccactaaggc atggagcttt tcatcatagt ttttaagcaa accaaacgct
    14581 tcgccattag ccgaagatac ccgtactgga aaatgattgg agctttcatt agaggatgac
    14641 agtaattcga ttttagacga agccgtattg acctcgatca ctcggccaat caatcctttt
    14701 tgcgacatga ctgccatatt tgcttcgata ccgtctttag aacccttgtc tacgatcagc
    14761 atgtcctgcc aactgtcagg cgaacgagta atgacatttg ccgttacttt ttcgtaacta
    14821 gtcagcgtct gattcaactc aagttctttt ttcaacgctt cgatttctct ctcgtaattc
    14881 ttgttttgct gtgaaacgtc atcatagcta tcgatttttt cttttagccg ctcattttcg
    14941 gcataagttg aaaaaagatt atggacagaa tctactccat ttcgtaccca ccgtactggg
    15001 gcagagattg ttttatcaac cattgcaaca ctgtcattaa cgatggattg gaagaaattg
    15061 ggctttcctt cattcgctcg ctgcgcagcg gtcacactta gtatcgtcac cacgatgata
    15121 acaataacca aggtaataat aatattttta ttaggattga acttcttcac aaagacacct
    15181 cgaattaatt agactcttgt gttcaatttt atcactaaat gccgtgaaac aaaaggtaag
    15241 ttgctcctct taagcttttt tttagcaaat aatagattct gtcattttta gcctgaaaga
    15301 caaatttttt tatttgttta tggatcaatc tttgctttga ataactgcaa tcataccatc
    15361 tgaaaaagaa aattctgcgg tatctgaaag aaattcgtta cttgcataag atgtcggata
    15421 ttctacttct ttattttcca atacatactt gctgcctttt aatgtaagat tcgagacggg
    15481 agtcaagcag caataagcta aatacttcat atctggtatc tgtcttacta tatgctctcc
    15541 aggcaaataa taagacaaat aattttgaca atctctgata ataagctgat gggcaaaggg
    15601 tttgaagcga ggttccatac ctaaccagag attggctaaa agatgatcca atcttccgcc
    15661 cgtagctcca atcaaagtga cttccgcttg cgggtaacgt tcaaatgttt ttaagatacc
    15721 taactgtgta tctgtatcgt ctttttctgc aggggaacgg gtatactctt tggcttttgc
    15781 aaatacttcc ttctgctctt cgttcgacaa tgaatcaaaa tcacccactg ccagatctac
    15841 tgggtagcct gcttgaagca ggtacagcgc accacggtct atcccgacat aggcatcgta
    15901 tttggacagc agagactgcg gccagttttg cggattacct ccagcagcta ataaaatttt
    15961 catttagtct aatgcctctt ttaatcgttc gatttgtccg actggatttt cggctccata
    16021 gatatatgat cctgcaacga agacatccgc ccctgcatct ttacagattt ttgctgtttc
    16081 tggaacgatt ccgccatcta cttcgattgc ataagtccaa ttgttctctc tacgtagatc
    16141 agcgagttcc gtaatttttt ctactgtttc ttcaataaag ctttgtccgc cgaacccagg
    16201 attgacagtc atgactagta cttgatcagc caaaggcaaa acatgtttga tcatggagac
    16261 tggtgttcct ggattgataa ctactccagc tttaactcct aagctttttg tcatttgaag
    16321 tgcccgatga atatgaggag ttgcttcgac gtggacagta atgatatctg ctccagcttt
    16381 tgcaaatgct ggaatgtagt tttctggatc aacgatcatc agatggacgt ctaacggtaa
    16441 tttagtaaca ggacggattg cctgaacaat atttggccct aatgtgatat ttgggacaaa
    16501 tcggccatcc attacatcta cgtggatgta atctgcgcct cctttttcca ctaactcaat
    16561 gtctctttgt aaattggcaa aatctgcact caaaatggat ggtgctatct tcattcttgt
    16621 ttcctcctat tttcgtttac cttctttttt tgcatacatt ggttttcttt tttcgatttc
    16681 ttgtaagaat tgcaaataat tgtcgtaacg tgtctgtgcg ataccacctg tttctactct
    16741 atgttttact tcgcagccag gttctttagc atgcatgcac tctctaaatt tgcaatggac
    16801 agaagcttct acaaattcag gaaattgtct cggcagttct cctgcttcaa ttgtcaaaaa
    16861 gtcaatcgag ctaaatcctg gtgtatctgc tactaacccg ccatagatag gaatcagttc
    16921 cacatgacgt gttgtatgcc gtcctcttcc aagtgcatca gaaatctctc ctgttgctaa
    16981 attcagttcc ggtgatattt gattcagtag cgtcgatttt cctgctccag actgacccat
    17041 aaagaccgat agccgttctg ggaagtaacg gatcagttct gtcgtatcac ccgtatgatt
    17101 gggtacaatc accggatagc ctattttagt ataggtttgc ttgatctctt cgattttttc
    17161 tgggtcgtct gacaagtcgg tctttgtcaa aaaaataatc ggttcgatcg actcgtattc
    17221 caaggtaact agaaagcggt caagtagatt ataagaaaat gcaggctcta cgatacttgt
    17281 gacaatgatt ccaagatcaa catttgtgac tggtgggcga actaattgat tttttctcgg
    17341 caagatttcc agaagatagc cgtctgtttg attttcactc tcaaagatca cctgatctcc
    17401 gacaagcggc gtgattttcc gattccggaa atttcctctt cctcttgttt gataggtctc
    17461 tccgtttgcg tatacatagt aaaaaccgct gattgctttt ctgatttgtc ctttgataag
    17521 tgccacctcc gtttttgttt attttaccat acgaagagga aagtaggtgt tattccatgc
    17581 aaacttctca aagtatatgg cttgaaaatt gatcaaaaga gatagaaaca caaaaaagag
    17641 aatgcaacaa atcaatgtcg cattcccttc tgatatattt tattcagcac ttattttgaa
    17701 ctttctgagg tgctagctga tgaacttgtg gagctggaag attcttcttt tgaagagttg
    17761 cttgtgctac tggagctact tgagctgcta gaactgtctg aagagtcttt ttcagaacta
    17821 gaagacgctg gatctggtcc tttagagtaa attacgttca caaccattcc tttggtaatt
    17881 gtcgttcctg cccctggtac tgtacgaatc actttatctt tatccaccgt gtttgaatat
    17941 tcggaagtct catgcccgat atattctgca ccgacactag ctaaataact ttgtgcttcg
    18001 ctttttgtat agccggagat atcattcaat ttaggctctg ttccttggct tacgacaaaa
    18061 ttgatgtaag tttcttctgc tacaacttca cccgaggctg gatcctgact gatgatcgaa
    18121 cctgatggta cagtgtcgct atagtcataa gtagcactaa tattattgat attgaatcct
    18181 tgtgtaacaa gccaattacg aacagtttcg tagttttctc ctacatgact actcaaagtc
    18241 actgtttctg gtcctttgct gactgtgaaa gtaatcgtgt ctgttttagg atcgacttct
    18301 gtatcttctg atggagattg cgaaatgatc attccgcttt ctacctctga actgaattcc
    18361 tcttcttttt gaatcaagtc agcagaaaac cctaattctt tcaaacttgc ttcggcatct
    18421 tcataacttt taccactgta atcatctaat ttgatttttt tcgtgccgct actaatatac
    18481 agagtgactt cgcgtttttg cttgactgtc gttcccgctg ccgggtctgt cttcactaca
    18541 ttaccttctt cgattttgtc atctgcaatt tcttctgttt ttgttgctac ttctaatttc
    18601 gcttttgcga gtgtttctct agcagaagct tcagaaagat cagaaacatc aggaacaaca
    18661 acttctcctc gcccgccaga agcaaagaat acagctgttc cgattcctaa aacagcaaga
    18721 atagctaata ggatcagcca ccattttttc tgtcctttct ttttaggatc aggcagaact
    18781 tcttcctcct ctggtaaggt ttcgttttct ggcttgtctt ttggcagagg catagatttg
    18841 aatgaatcag gcattggtgc atcttccgta aggggtgtga taacttttgt ttcattatcc
    18901 atcacatgag gctgccattt ttcttcgttt gcacgagctg gtgataatac tgtagaaaga
    18961 tcatcagaca tttcctcggc tgacttataa cggtctgcag gttctttagc cgttgcccgt
    19021 aaaatcacgt tttccagtgc ttgtggcaca tttggatcaa gtgcttttat agatggaaga
    19081 tcatcttgaa aatgttttaa agcaattgtt acagcagatt caccatcaaa cggtacgcta
    19141 cccgtcaaca tctcataaag gatgatccct aaagcataaa tatctgactg cttcgttgcc
    19201 atacttcctc tggcctgctc aggggacaga taatgaacag atcctagcat cgtattcgtc
    19261 tgcgtgatcg atgtttcaga taaagcgata gcaatcccga aatccgtgat ttttacaaca
    19321 ccttcattat cgatcagtac gttttggggt ttcaaatctc ggtggatgat ttgatggtta
    19381 tgagctaaag aaatagcaga aaggatctgc tgcataatat ttactgctgt ttcataagga
    19441 acaggataat gtgtttggat atatcgtttc aagtccattc ctttgacgta ttccatcacc
    19501 agatactgca tgttgtcttc ttcaccaaca tcgtaaacac taacaatgtt tggatgaacg
    19561 agttctgttg ctgctaaagc ttctctctga aatcgtcgaa tcgcagtctg gtcattttga
    19621 aagtcaaatc gtaagacttt aactgctaca tcacgatcca agattaaatc gtgcgccaaa
    19681 aatacatttg ccataccacc gctaccgata tttccagtga tttgataacg gccatttaat
    19741 tttttgccca actcgatcat acactgtcct ccttatattc gataagaaga acggtgatat
    19801 tatccgctcc gcctgcttca ttggcacgat caatcagctc agttacttta tgagacaatg
    19861 ggccttcttg attgatgatc gttcctattt cttcttcaga cagcatgttc gtcaagccat
    19921 ctgaacataa cagcagtcga tcttttagtt gccaaagatg agtagaaaca tctacttcga
    19981 ctgttccagg catgccgaca gaacgtgtta aaatattttt ccttggatgg ttcactgcca
    20041 tttcttcgct gatttctcct gatttgacta gttcattgac taaagaatga tcctccgtca
    20101 attgcatgat tttatcttca cgaatcaaat aagctctgct atccccaaca ttggcaatcg
    20161 tgaaccagtc atctgacaga gcggccgcta cgattgttgt tcccataccg ttatattccg
    20221 gctgttcttg tccacgctga taaatcagca tattttcttc ttgaatcgat tgaatgaacc
    20281 attgggcgat tttttcatcg tcaacagctt cttgtttttc ccaagcttcg cctagattcg
    20341 ttaccgccat ctgactggca atgtctccgg cacgatgacc acccatcccg tctgctaata
    20401 cagcaagctt gagacctgaa cggctggtaa atacactggc atagtcttga tttgtgttac
    20461 gtcgtcttcc tacatctgat tgatattcaa tctgcatttt ttcacctcat tactttttgc
    20521 gcaaacaaca aatgaaaaat ccatctgtgt aaaactgatg cggatacaaa gtcaacattt
    20581 tctcttcgat tgatgattgt agcataggat tggtcaaaac atcaatcctc tcaaaatcag
    20641 ggtgtctttc caagaaagca gaaacaacgt cttggttttc ttcttttaag atcgtacaag
    20701 tgctataagt cagaataccg gaagacttta aagttggtgc gcagctttct aaaatttcca
    20761 attggatttt tggtaaattc gctaattcat ccggctgttt tttataacga atatctggtt
    20821 ttcgccgaag cagcccaaga cctgaacaag gcgcgtctac taaaatccga tcaaactgct
    20881 ctgcttcgaa ctgtttactg acttctctag catccatctt ttgagcgtag acagcagaag
    20941 agacacctag acgtgctgca ttatcttcaa tgagtttgac tttgtgttcg tgtacatcta
    21001 aagcgaccac ttctccaccg catgacggat ctaaaaaaga agcaatatgc gtcgtcttac
    21061 ctccgggtgc tgcacaggca tctaagacat gatgttcagg ctcgatttgc atagacggcg
    21121 ctacaagcat cgaactttca tcttggatcg tcatgacgcc atcgtggaac aatgagctag
    21181 aagctaaatg cccttttttc gctactatac cgtatggact gacttggctt ttttcagctg
    21241 tgatctcttc ttcttgcaaa acagtgatgg cctgatctct ggaaagtcgg cgtgtatcta
    21301 ctcgggcact agcatggcta ggctgatata gcgataaacc gagcttttca gtttcttcgt
    21361 atccgatttg atccaccagt cgtttcgtca tccatagagg caggctgatt ttgacagaaa
    21421 gtctttcgat aggatcagca atgccttgca gatctggtgc tccttgacgc tgataattcc
    21481 tcaagacacc atttacaaat ttccctgtcc caggatttcc ttttactttt gcgatttcga
    21541 ccgcctcgtt taaaatagca tgtgcaggga ctttatctaa aaattctaat tggtatacag
    21601 aaagataaag aagcatcttg acccagtcat ccactttttt tgctttagcg atcaatggtg
    21661 caacgtaaaa ttccaatagt cgctgatggc taatcgtacc atagatcaat tctgtaaata
    21721 aacccacatc tttttgattt agctcgcttt gtttgatcat ctcattcaac aaaagattcg
    21781 agtaggcgcc acccttgttc actcgctcta atgtcagtaa tgccatatat cgtactgatt
    21841 ttttcatttt tttagggatt tttttcatta tttcacctgc tgaccaatct gaacttgctg
    21901 accgcttccg ttaaggaaat catgtacggc ctgttttcct ttaccagctg gctgtaattc
    21961 attgatggct aagacggtct gtttaccgca agcaatccat agattttttt tgtctttccg
    22021 gatgatcgta ccaggtgctt tgtctgtagt ttctgccaat acttcaaccg ataataattt
    22081 ccagcgtatg ccctcatagg tcgtgaatgc aattggccaa ggacgcattc cgcgtacttg
    22141 gttgtcaatt tcttctgcag ttttatgcca atcgattgct tcttgttccc gtgtgatgtt
    22201 tggcgaaaaa gttgcttctg actcgtcttg aggtactggt ttttgtccat ccaaaatatt
    22261 gggcaatgtt tctagcaata gctttcttcc taaaaggcta agcttttcaa acatcgtccc
    22321 tacatcatct tgtttagtga ttggtatgct ttcttgcgca tagattccgc ctgcatccat
    22381 ttttttgatc atttccatga tcgttacacc agtttctttc tccccattca tgatggcata
    22441 atgaacaggc gcacctcccc gatatttagg aagtaaagaa gcatggacat tgattgcccc
    22501 taattttggt acttgcaata acttttcagg caagaattgg ccaaatgccg cagtgacgat
    22561 cacatcaggc accaattcaa tgatttcttc catttctggc gaaccggaga ttttctctgg
    22621 ctgcagcaca cggataccgt gcttcagagc cgcttttttt accggtgtcg gcgtaattac
    22681 tcgtttccgc ccaactggac ggtcgggctg tgttaccact gcctggatct cgtaacctga
    22741 ttctatcagc ccttcaagaa tcggcacaga aaaagcaggt gtgcccataa atactagctt
    22801 agtcatcaag atgttcctcc atatattctt caagttcttc ttccgggatc tgctgtatca
    22861 ttttctcaat gaacaagata ccgtctaagt gatcgatttc atgctggaag gcacgggcta
    22921 aatagccaaa agccgtcact tctatctctt ctccgtctcg atcataataa cgcacggtca
    22981 cttcatctgc tcgcttgacc gttccataga catgcggaat actgagacaa ccttctacgt
    23041 caaggctttc accttttgct tcgatgattt ctgggttaat cagttcaaat ttctcgcctt
    23101 catctacttc gatcacagca attctcttgt tttgtcctac ttgaggagca gcgatcccta
    23161 ttccgtcatt cgcaatcatt gtctcatata aattgtctaa taaagcgatt gtctcgtccg
    23221 tgattacatc gattggctga gcagttcttt ttaatttatc attagggtgg atcaatatag
    23281 gatagcgcat aatttggccc tttcttaaat aaagtttaat ggttcgttgt caattgccac
    23341 aaaaagacct tgtcgctgtt ctttctggct tccattcaaa atttcatcca aaacatgagg
    23401 aagattcggt tcatgtttat atttgatgat tagttgataa taatatcggt ttttcactct
    23461 caaaatcatg ctgggcgttg gccctaatag caagctttga gggcttaaca cttcttttag
    23521 ctgattggca atctgaaaga ttttctttgc cgctacttgt tcttcaggat ggctggcggt
    23581 tattttcaca gtaaagtaat aaggaggata gcctccacga tgccgtacat gcatttcctg
    23641 ctgataaaac tgctcataat tttgtttttt agccaattct atggcgtaat gttgaggatt
    23701 gaatgtttgg atcacgactt cgcctgcttt ttccgctcgc cctgctcgtc ctgaaacttg
    23761 ggtaagcaat tggaatgtat gctcactaga acgaaaatcc ggtaaattca gggaggtatc
    23821 agcattcaac accccaacta atgtcacttc tggaaagtcc aaacctttag caatcatttg
    23881 tgtccctaat aatatatctg cttcttttgc accgaatttt tgtaaaatct gttcgtgtgc
    23941 cccttttctt cgagtggtat ctacatccat acgcaaaatc ctagcttgcg gataaagttc
    24001 tcggagttcc tcttctactt tttgagtacc tgttccatag tagcggattt tattgccgcc
    24061 acaattagga cagcgattgg gaataggttc ttcatgtccg caataatggc agcgcattga
    24121 acgtgtatcc atatgcagtg taagagagat atcgcaatta ggacatggaa gaacaaatcc
    24181 acagtcccta cacatcacaa aagacgaata tcctcgacga ttgagcagta ggacagtttg
    24241 ttcttttttg tctaatcgat tttgtatttt ttcctgcaaa ttagcagaaa aagtagaggt
    24301 ccgatgattc tgaaactctt cacgcatatc tacgatctca atagctggaa gctgtgcagc
    24361 tgctttggct cggtggttta actgcaataa ctgataaacg cctttttgag ctcgtgctct
    24421 tgattctaaa gagggcgtcg cgcttcctag aactattggg caatgatgat atttactgcg
    24481 ccaaattgct agatctctcg catgataacg cggtgtctca tcttgtttgt aacttgattc
    24541 atgttcttca tcgataatga tcagcccgat atttttcagc ggggcaaaaa tagcagatcg
    24601 tgcgcctact actacttgcg cttcttcccg ctcaatcttg cgccattcat catatttttc
    24661 accttgggaa agtccgctat gtaagaccgc aactgattct ccgaagcgtc ctttgaatcg
    24721 ctcgaccatt tgcggagtca atgcgatttc aggcactaac ataatagccg ttttattttc
    24781 tgacaaaaca tcagcgattg cctgaagata tacttctgtt ttcccgctgc cagtgattcc
    24841 ctccagcaaa aagacctgat ctttttgctg ctgacctgct gtgatgattt gttcgacagc
    24901 atttttttgt tctgcattca gttccaatgc cgtcgtttgg tcaaatactc ggtctttata
    24961 agggtcgcga taacgttctg attctataaa ctccagccag cccttttctt ttccttgatt
    25021 caagacagca gtactgattc ctttattttt gaaataagca attggttctt cttggctcag
    25081 ttcagataaa cacaaaagaa gttctttttg tttcttggca ttttgccgta attttgccca
    25141 ttcttcttcg atttgctttt cagttaacaa agaacggacc aaacgaatcg tttttacttt
    25201 atttcgtgta tggacttcat atttgatatc tactaattgc tcttggcgcc agcgaatcaa
    25261 ttgagaaagg ctgccttctt cttctgcttc cttccaggaa atcacttctc gctgatcaaa
    25321 caatacctga acttctggtg tgtctgccaa aggatagatc aatttatcat attctgcttt
    25381 catcacactc ggaagcatcg tttgtagaca agtgattttg aatgcgtaag taattttttt
    25441 catataatca gctaatgaaa gtaattcttc attgaccacc ggagcaaggt ctagcaaacg
    25501 aataactgcc ttcaaagatg gattagctga ttcttctgat ttttggatag ccataacaaa
    25561 tccttggaca tgacgattgc cattaccaaa agggacttct actcgcatcc cgacttgcaa
    25621 agctgtttcc atttcagagg gaaccaaata agtgaatggc tgatcggtct gcatagtagg
    25681 tacgtccaca atgacttcag ctgttaatgt catggttcgt ttcctccttt ttttgtcttt
    25741 tcttatcaag cgacaaaaag ttccttgcct attttactag agatcgatgg atttgtctta
    25801 tcttctgcca aatcctaaca aaaaattaac agcagataag aaaaaaaaga actgtgtcaa
    25861 aaagttcaga cacagccttt tctaccagac aataagtggc gaatgtttga aaaggaaaca
    25921 gctgggtttg cttttctttt tagatgttcg ctctattttt ctttattaca ttttttgctc
    25981 ttcacggata cgtgattcca attcgtgttg ttcacgctct ttttgtgctt gacgttcctc
    26041 ttcttccatc ttcaaacgtt cacgttttaa ttcaggatgt ggatcattga ctacatttcc
    26101 tgcatcgatt tcttctaaag cttgaccgac gctttttact gaatcaaaag aatctagagt
    26161 aggctgtgca cttgcatcca attcatgcgc gcgtttactt gctaaaatca ctagagaata
    26221 ttttgaattt acacgatcta atagtgagtc aattgaaggt tttaacatca taagactaca
    26281 tctcctttaa cattttaata tatttaccga ttacgcgatc tactcgaaaa tgttcactgg
    26341 caataatgtt tttgattcgt tcaactgcta gcggcacttc atcatttaca acagcatagt
    26401 cgtaaagagc catcatttcg atttcttctt ttgcaacttt cattcgtttt tcgattactt
    26461 catctgcatc tgttcctcgg cctacgatcc gtgattttaa ttccgctaaa tctggcggag
    26521 tcaaaaagat gaatacacca tcgggcactt tttctttgac ttgttttgct ccttggactt
    26581 caatctccaa gaaaacatct tttccttcat ccaatgtctt gttgacatat gacaaaggag
    26641 ttccataata attcccgaca tattctgcgt attccagcat ctctccagct tcgatcattg
    26701 attcaaactc ttctttgctt cggaagaaat aatcaacgcc ttcgacctcc ccttcacgca
    26761 ttttacgggt agtcattgag atcgaatact gaaaatcatt atcctcactc tcaaaaattg
    26821 cttttcgtac tgtgcctttt cctacaccgg atggaccaga caacacgatt aataatccac
    26881 gctctgacat gacgacgtcc tttcaactag tctttttgta cttatcgttt attttgcact
    26941 ttttttctgc aactttcaag gttatcttta attatagcta gaaattggta aaaaagctac
    27001 cctgtaccaa gaggtcgtaa aaaagctgtc ctgcatcaag taataagaac agccaaacat
    27061 aaacagcttc tcatgttttt cgtttggttg gggttattat cgcagatgca agcttttgaa
    27121 cgccgtgtat aagaggtcgt gaagaagccg attagtttcg agtatgaaga aaaattttgt
    27181 gaaaatagtt tctcacattt ttgcataaat ttggcttaat accgagaaaa tggtttttga
    27241 acatcactta ttcaaaataa aaaaagattg tgacacgaat tttgtcacaa cctttaaatg
    27301 tattcctaga gcaaagaagc taagcgaaga aatccaattc tgtttattta ttgtatttct
    27361 ttgttcagca tccttgctaa aagcccatat tggacaaagt attcactaca cccatgatct
    27421 catcttttcg agaacgtgct ttttctgtcg gatcttctac ctcttctgtg tcaattcgaa
    27481 caataaattt ccgattcatc gtcacgtaag caccgtcgtt gatcaaaata aacagttcgt
    27541 tctcattata gtctgcacag gcatctttcc atacgtcaaa gccgtttttt tcagaaggga
    27601 tatcccgttt aaccacagtt tttccactta attcgtggaa cgttacgtca taatatttca
    27661 ttatctctac ctctttcgtc attcaatatt ttgtacttgt tctctgattt tttctaagat
    27721 cgtcttagac tggacgactt gatttttaat ttcgattgca tttgatttgg aaccgatcgt
    27781 gttgatttcc cgattcattt cttggatcag gaaatctaat tcccgaccta caggacattg
    27841 tgcttcgata agttcatcta gtttttgtac atggatcgcc agacgatcca gttcctcgtg
    27901 aatatctccg cgttccaaca agatggctag ctccgtcaac aaccgttgct catcgacttt
    27961 cgctccaaga aattcctcca gctttttttg ataccttatt tgaaattcct tttcatagat
    28021 ttctacaaaa ctatcgagtt tttttagtac ttctttgaat tcttcagtat tctttttcaa
    28081 cactaaagct aaagcttgac cttctttttc tcgactcgcc tcaatagaat caagtgcgct
    28141 tttcaatgta tctagagcta actgtgccaa ttcttccatg ctctcttccg gtttttcttc
    28201 taccgttacg aagttttcgt ccagcacgaa gcgttccgcc atccgactaa ttgacacttg
    28261 attttgacta ccgtatcgtt gttgaattcc gtctgtcaat tgagtgataa gtgaatcaat
    28321 caattcccag ttgatgacga tctgtttttc gtttttgcca atataagcta gattcacaaa
    28381 tacttcgatt cgaccgcggc ttaactgctg tttaataagc tgacgaaaat cgttttcaac
    28441 aaagttcagg attttaggac ttcgtatttg aacatctaga aaacgctggt tgacactttt
    28501 tatttcaata tctaactgat acgcttttgt ttcgcggatg gctttgccaa aaccagtcat
    28561 acttttcatt cgacagggtc cttcctcatt acatattttc tattgctttt tgcagttgtt
    28621 caaactcttc gttcgtcaat gtcaggcctt tccccatttt ttcatggtct ggcgcccaat
    28681 ctcgaagatc gaattttggc gggcgaccat tccagctgac taggttcagt tctttacgcc
    28741 accccttgtt gttttctgac aatatagcga tttcttctac gatttcatat gaaaattctt
    28801 gtgccatggt tgttcctcct tgagataata taaatatagg atcagtaatg tgtcaactgg
    28861 ttgcttaagc aattcatgct gcaaacgatc cttgcgtttt ccttgtagca gataatcaaa
    28921 ataaacagtt ttcgtgcgct cattttgtat tttttgctgg tattttcgat accttcgcca
    28981 ttcttcagta cttaattgac caatttgtgc caataaatca tccggcatac tttttatatc
    29041 ttgttggcag atcatttgaa tcatcatgtc tacttgatag atttcattgg gcagtaaaaa
    29101 atgttttttt gctaaaagtt ctagcatcgc caatccgcat gtctctggta cgaccctgtt
    29161 cttatataaa ttacgcagtt tttgccaaaa ttttatgctt ttgaaaaaag ataattctaa
    29221 ctgaatttct tttgttaagt aagacaaaaa tcctcgccag tatgttcttg caggtttaac
    29281 agaatgaaat gtataccagt tcccatagta atagcctaat cgttttttca tttccagata
    29341 gcctaattgt aaatttaatt ggttcctttg gctatcaaaa agcagaaagc tgcctagtgt
    29401 ccacaatgtt tgacatttcc aatggataaa aatgtctggc atacgaattt ttttcgctgg
    29461 gcctggccct tgcacatcta cgacaaatgc ctctgttgct cctttgttaa tcgcaatatc
    29521 aataggtatt ttgttgcgat acccaccgtc tgcatattta ttttttccga tctttcgata
    29581 agccatagcg ggataaaatg aagcagaggc gagtatccaa tctggtattt cttctgtttt
    29641 tgtctgttgg atatcagtca ctacttccgc tagttctggc agtttagtag acaccgtata
    29701 taaacgtatt ttgggattat gtttgagtaa ggacaaatcc aaattgtctt tgaccatctt
    29761 ttccaatgga gcagaagaaa ccccgccttc aattagtgct gttttcgtca tttggcgtct
    29821 ttctcgatag aagcgttcac gaaggtcttc aactgcagcc atttctggta acgcaagcac
    29881 ttgtcttgtc gacagctttt tccataaatt cacagctttt ttcatatctc cttgtaaaat
    29941 caatgcgccg ttcaacgcac ctaccgaggt acctgtgata atctcaaagt tgatatcatg
    30001 ttccttcagc gcctgccaca caccaatctg ataagcaccg tgcgctcctc ctccacctaa
    30061 aacaagtgct gtatgataga ccagcttttt atttaattgt gtggttcctt gatagccgtt
    30121 tttatgaaaa atacgcaacc attcttctga tagaggagat tgaaataaaa aacaacagtt
    30181 ttctttaata aaaaaacgag cgcaagcttc catataccga aaaaattttt tccattcaaa
    30241 agtttcacct tcaatcaaca gattattaaa aataacatct cctctacttt tagcaatagt
    30301 aaaatagacg gtatattttt tcgatactgc tttaaaagca cattgctttt ttgcagaacg
    30361 ccaaaaagcg tctctacttt cttgatgact ttgaaaaaat ggaaggggtt gttttgcttg
    30421 ctggataggc gaatacagcg acattttctg tgaaaagtaa acttttttta tctccatcgc
    30481 gctctccttt ttcttcaagt ataccagtac gtcataaaaa ggtcatgagt ctcgtattcg
    30541 actttgtaga ttccataaga aaacaattca taaaacctcc tttaaataaa aaaatacaaa
    30601 aatcacaaaa attaattgaa aatgagagtg ttttcaatga aaacgtgatt ttgtaatgga
    30661 attttaaaat aaaagaaaca agctttttta atgcaaatgt tgtaaaataa aagagattaa
    30721 gaggaaataa gaggaggaac atgatgactg agtcagttta tgtacatatt gataccacaa
    30781 gtaatgctgt tttaacaaaa gggctgactg ctactgactt tgcaaacagt atcgtgcatt
    30841 ttcctcaaaa cttgctcttg cttgatccat ctgcttctgc tggagaatat gaatcccata
    30901 cagggctgaa agtaatccgt ggtacagaaa atattcagcg tttcttttcc tcttctcgta
    30961 acaggcgtgg attcgatgaa ttgaagtgga tcgactttac tgatttaacg atgctgaaag
    31021 aattgacacc tttagaaatt tcagaattat tatattttgg tcatatgaaa acacatcttc
    31081 attcgccgtt tttttataaa ctacagaata attttgtata cttcgatctt aatgatcagc
    31141 tgaatcggat ttattatcgc tatttagatg aattttatag gatttttgct agtaaattaa
    31201 gttcgatctt gtcagataag atcaatgtaa aaaaatcttt tttcaaaaaa gaaacacctg
    31261 ttgaaaaaat cagtgtggaa ttattgaaag aaatgcgtaa tttgatgcag gaagggatca
    31321 ttttttcttt tgatcaagtt ggtttgatcg atggtaaata tcgtatccca atctatgtga
    31381 ttgaagatta tatttggaaa ttaaaaggca cccggtatcg agaagaggaa atcatcgcta
    31441 ctttaatcta ctctgtcacc gatcgacact ggcaattggt agaagatgcc gatctttctt
    31501 atctgccatt gaagtagatc tatttcttat caaaaaataa aaaggaaaca agaaactagg
    31561 acatgattct gtctcaagtt tcttgcttct tttttattta cagctcctgt cgtttatctg
    31621 gcacaaaaat cgaaggagtc gtttgttgga tccctaataa aacgatgatc gttacaatag
    31681 ctgtactcaa cccgctggca ccattcatta cgaaagaaaa gagccaagga ttcatccccc
    31741 atagtgcata cgctccccag aaaaccactc ctgcaataaa atgccaaaaa tatctggaaa
    31801 gtgcgccaat caatgcacca aacacgatgc tttttctaat ttgtttcgta ttatcgtcta
    31861 catagccttt ttggacattt gaagccatga gccctgctgc gcctgcaaaa gtataggcaa
    31921 tcgggtactc aatcaacact tgaacgacac ttaaataata aacttgtccc aacggaaaat
    31981 gtaggatgcc ccaaatgaaa cctgccagca tccctgcctt tgtccctctt cttaacgcaa
    32041 aaagagtcaa tggaatctgc cctaatgaaa tagaaaaact gctgccgatc tggatcggaa
    32101 tcaacgacaa caccatcgct aacgctgcaa caatcgttcc ttctacccaa actctatttt
    32161 ttcccataaa aaaacttcct cctttgaata aaaaagtcac aaaggaggaa gcagctttcg
    32221 aaaatagtca aacagctgtt tattcacaat tcctacgctc gcataaccga tacaggtacg
    32281 aagggtttag aaatagattc aatctcagcc caattggact cccctttgtg agtttattta
    32341 ttatttattt tttgtttcaa acaatggact aactggtttg ttttcatgga tacgtttgat
    32401 tgcatctccc attagtccgc caacacttac ttcatcgatt ttgtcgattt tacgatcttc
    32461 tggaagatag atcgagtctg taacaactag acgctcgata gctgaatctt ctattctttg
    32521 taaagcaggg ccggataata ctggatgtgt acatgaagca tacacactag ttgcaccagc
    32581 ttctttcaaa gcattggcag ccaatgtgat cgtacctgct gtatcgatca tatcatcgat
    32641 cagtacacaa gttttgcctt tgacttgtcc aatgatattc attacttctg caacatttgc
    32701 ttttggacgg cgtttgtcta tgatcgcaat cggagatttc aaaaattccg ctaatttacg
    32761 tgcccgtgtg acaccaccat ggtctggtga aacaacgaca acatcatcgc ctttgattcc
    32821 tttttctaag aaataattgg caataagtgg cgcacccatc aaatgatcga ctggaatatc
    32881 aaagaaacct tggatttgga ctgcatgaag gtccaaagtc agcattcttg ttgcaccagc
    32941 tttttgaatc atgtttgcta ctaatttcgc tgtgattggt tcccgtgcac gtgctttacg
    33001 gtcttgacgc gcatagccat aataaggcat gactacgttg attgtttttg cacttgctcg
    33061 tttcagtgcg tcaatcatga tcagcaactc catcaagtta tcattgactg ggctgctcgt
    33121 tgattgaata acatagacat gcgatccacg aatactttct tcaatgttta cttgaatttc
    33181 tccatcacta aattgagtaa cagatgattt tcctaactcg acgccgactg ctgctgcaat
    33241 tttctctgct aatgggcgat tggaatttaa cgcaaaaatt tttaatcttg ggtcaaagta
    33301 atgttttgac atggggacct ccactttctt tttcacaact ctagaattct acctaataaa
    33361 tagtagtcat attttttaaa taaatcaagt gattttgcaa aagtcttagt ttagataagg
    33421 gagttttttt gcatatcctt ctttattgac ttgtcttgct ctcgcaattg ccagcgcata
    33481 ttctggaata ttgtctgtaa ttgttgaccc ggcagcaatc gaactgtttt tagctacctc
    33541 gacaggtccg atgatattcg tgctagaacc aataaagctg tggtcgccga cagtcgtatg
    33601 atgtttgttc tttccatcat agttgacgaa gacaacacca catccgacat tgatttcttc
    33661 tcccagcgta gcatcaccaa cataagtcaa gtgaccgact ttagttcctt tgccgatttg
    33721 agcatttttc acttccacaa agtttccgat atggacgttt tcgccaattt cagcttttgg
    33781 tcgtaaatgt gcataaggtc ccacatctgc atgtttcttg acatggctgc tttcaatgac
    33841 tgagttttca atgacgacgt gatcctcaat cctactgtct acgatttttg aatgtgaccc
    33901 gatgacacaa tcagaaccaa tgactgtatt tccttggatt tgtacacctg cttcgatcaa
    33961 tgtgtctgga ccgatttcta cgcctgcatc gatgtatgtc gttgctgaat cgacaaagct
    34021 tacgccattg accatatgca tctggttaat tcgctttctc atgatctcat ttgctttagc
    34081 taacgcgatc cgatcattga ctcccattga ttcttcaaaa tcatccgttt gataagcagc
    34141 aactgtatgg ccttcttctt ttaagatttc gatgatatct gttagatagt attcaccttg
    34201 agcattgttt gttccaactt tgcttaacgc atcgaacaaa gcttcgttgt caaaacagta
    34261 agttcctgta ttgatttctt gtacaagagc ttcttctggt gttgcatctt tttgttccac
    34321 gattttttct acgatcccaa tatggtcacg gatgattcgt ccatatcctg ttgggttttc
    34381 tgcttgtgcc gtcaagatcg ttgcacttgc attttttcct tggtgatatt caaaaagatt
    34441 gttcagcgtt tcagtagtca ataatggtgt atcgccactg ataactaacg ttgttccttt
    34501 ttttccttcc aagaaagaag cagcttgcaa aacagcatgt cctgttccca attgttctgc
    34561 ttgtaacgca tatttactgc gttctcctag ttgtgcttta acttgttctg ctccgtgtcc
    34621 aacgatcgta atcacttcat ctggtttcgt ttcactgacc cgattaatga tatgttctac
    34681 cataggttta ccagaaacgg gatgtaaaac tttatataat tttgacttca tgcgggtacc
    34741 tttccctgcc gccaaaataa ttgcataacg tgcgtccaac tgcgtcactc ctaaatatag
    34801 tttgctgatt tcagtttatc ccagtatacc cattttttca acaaaacaag tgattttctt
    34861 ttatcccagc gttttctgga taaaaaaata gatagaccat aacgggccta tctatttggt
    34921 aaccggtgct ctcctgccaa ctcctcggtc acaagtcaca ctactcataa aacatgaaaa
    34981 gctgtttttt gaaatcgttt cttgtgactt agagttaaac ggtaggctta caacctcttc
    35041 attaaacgtt tttcaatcag ctgaccttcg gtattagctc aataaacggc aaaatatgag
    35101 gagcaatttt tccgtttatt gagctaatac tcaggacagg acgctgattt cacaacctct
    35161 agataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg aaaaaatgtg
    35221 gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcggcttt ttcacaacct
    35281 cttattctga aaaataattt cctggcacaa ccgtaatatt tttcgtttga gtatctacat
    35341 ctttgacata aagcagtgac gtataatcat cgatggcgcg atgaccgttg aattttgatt
    35401 cggcaaatac agtgatgcca acaagttctg attcgaattc ttcaatcaag ctcttcattc
    35461 cgttgactgt tccgccgcct ttcatgaaat catccactac aaggacacgt gaaccacgtt
    35521 tcaaacttct cttagacagt tccatctttt caattctttc agaagatcca gacacatagt
    35581 tgacacttac tgttgatcct tctgttattt ttgagtcccg gcgtacaata acgaatggcg
    35641 cattcagata ataggacaca gcttgggcaa taggtacgcc ttttgtggct acagtcatta
    35701 cagcatcgat tttttcacca aggtatttag aagcaatgat tcttccaact tgacgcagca
    35761 gttcaggttc tcctaacaaa tcagagagat aaacatatcc tcctggaagc agacggtctt
    35821 gttctgatag acgttctgct agagattcaa tatatttttt cgcctcttca tatgaaattt
    35881 cagggatgaa caatacacca cctgcagcac ccgggattgt ttccaaggtt ccataccctc
    35941 gttctttaaa tgttttcttg atgattccca aatcttcact gatagaagat ttagcagatt
    36001 cataacgttc ggcaaagctt gttaaggaaa tcaattcatg cgggtgattc aataaatact
    36061 gtgtcatgtc aactagacgt tcactgcgac gtactttcac ctattccaac tccttttaag
    36121 aaattttatc gttttacatt atataagttt ttcttcaaaa attcgagcat tttatgaaaa
    36181 aataagaaaa tagttcggta tttattaaaa taaaaagaag cctataaaaa atttttcgtt
    36241 tttataaggc ttcttttctt tataatttag ttattttttc ttctgaaggc aaccaatatt
    36301 cgtttgatga tatttacaat caggaacaaa gcaataaaga ctaatgtgat tgttgctcct
    36361 ggaggtgtat ctagataaaa cgagctagtt agtcctgaca gcatccctag aaatcctaca
    36421 caaacactga tagcaatcac gacattgaag cttttgccta atcgcatacc gattgctgcg
    36481 ggaagaacca tgattgcgga tatcaaaagt gcaccggcaa tcgggatcat cacagcaata
    36541 gccacaccag taatcacatt gaacatcatc gacatcaaat gaacgggtag cccatccacg
    36601 tgggcggtat cttcatcaaa agtcagaaca tacatcggcc gtttgaataa aaagaacaat
    36661 ataccgatgg caacgaataa gattcctaaa aagatgactt gctcccatgt aatggtcacg
    36721 atcgaaccaa acagatagga ttggatactt gttgctgaat tccctccgct caaattcatc
    36781 aatactaacg ctagcgccaa accaccagcc attaaaatgg caatggagat ttctgaatag
    36841 gttcggtaga tcgttcgcag atactccaat atcacagctg ctaatacaac gactagcatg
    36901 gtcgtaagtg taggattaag gttcaaaaag aatcctaacg ctacccctgc taaagagaca
    36961 tgagaaagcg tatcagccat cagcgattgt cttctgatca ctaaaaacac acctagcatc
    37021 ggggcgattc cggcaatgaa gatagcggca agaaacgctc ttctcatgaa ttcatatgaa
    37081 agcaacgcca ttgagaatcc tcctttctca ccagtcggat ctgtctgtcc gcatactgtt
    37141 tgatatcttc atgatcatgg gtgatcatta gaactgattt cccatggaca tgcgcactat
    37201 gtcggagaag acgataaaat tcatttctgg attcttcatc catacctgtt gttggttcat
    37261 caagaacaaa taagtcaggg tcagtagcaa aaatacgcgc taaactgatt cgctgctttt
    37321 gtccgccaga caattcacct attcgccgat ttcgcatatc ccacatccct acagcttcta
    37381 aagcacgctg tacatgttga tgatcttttt cagagagacg cttgaaccag cggcctcttg
    37441 gatatcttcc tgaacggact aattcgatca ccgtactagg aaaacctgca ttgaaagatg
    37501 caacttgttg aggaatataa ccgatactta atttttttcc ttcttgattt ttctcagcaa
    37561 tctttatcat tccttttgaa ggtttcagta atcctaacga tgctttcacc aaggttgact
    37621 tggctgctcc attttctcca gttaaaatga caaattcacc cgcatctaca tgataagaga
    37681 catcttctag tacaggttca tcatcatagt aaaaggtcaa atcagatatt tcgatataac
    37741 gcaccttaca acctccattc taaaacttaa ttgatacttt cttttaaagc tttcagattt
    37801 ttttccatga cagaaatata attttcgcct gctttgattt gttcattagt caagctttct
    37861 aaaggattca acacttctag cttcactcct gtttcattcg acaatgtctc agctaccttc
    37921 gaagaggcat tttcttcaaa ataaatcact ttgatgttgt ttttcttcac aaattctttt
    37981 agctcagcta agcgacttgg tgtgggttcc tgatctggtg aaacgcccga aatcgctact
    38041 tgttttaagt cgtattgatt cgctaaataa gcaaaagcag catgctgagt gacaaatgtg
    38101 cggtttgttg catcttttag ctcatcggag tatttttgat ccagtgcttc aagctttttg
    38161 atataagcat cggcattctt cgtaaaagtt tcctttttat ctggatattt ttcgcctaat
    38221 tcttcagcaa tcgttctgac ttcttttata gctaataccg gatcgagcca aacatgtgga
    38281 tcgtattcat gtgaatgacc atgcccttct tctgattctt catgttcgtc tgcttcttgc
    38341 gattctaaac ggtcgattcc tttagctgct tcgatcatca aggtctgttt gctatccact
    38401 gaatccttta acgaatccac aaaaaattcc atgtctgaac tattatacac gaaaacatct
    38461 gcatcggaaa tttctgctct ttcttttgca cttggttcga aatcatgcgg ctccgttcca
    38521 gcagggatca aaagtttcac gtttccttca tcccctacga tctcttttgt aaaatcatac
    38581 atcggataaa aagtcgtaac gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta
    38641 tttccacatg ctgcaaacag taaagcacct atcattaccg tagctgtcaa aagatatttt
    38701 ttcatttata tgctcctttc aatgaacaaa tcggaatttt acgatttatt aacgtgaact
    38761 aatgtatcaa aataaaaaaa cacagtcaat agaaatccat caatttctga tttaagcatg
    38821 tatattcttg attaaatcgt aaaatggatg agttgctgct catatctagc catttcacta
    38881 caataaaaaa ctgaagaata ccattttagg attcctcagt ttttcatcac ctattttact
    38941 tcttatattt tattgcaggg ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc
    39001 attatatact ctctgcgctc ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct
    39061 tccactcatc aatgcagcat ctgctccgta cttgatcata cgctctttga tttgatggat
    39121 gactggatgc cgctttcccg ttatatcttc taaactgttt cccatttttt tgatcatctg
    39181 ctgataatct tgctctcgga tggcttcagc aagtccgtgg atgtcttgat gttggatggc
    39241 ttgcgtatct acttctctaa acactgtacg tgtagagaca ctcattcttg gtttgaccaa
    39301 cacaacccag cactgtggca tagaaggtaa tacttggatt tcttcccctt tgccaccgac
    39361 aaatgccgta cttccgtaga cacagtaagg aacatctgta ccgacttcca aaccgatttc
    39421 tgccagttct tctaacgaac agcccagatt ccataattgg ttgatccctc ttagagcagc
    39481 tgcacagtct gtacttcctc cgcctaatcc agctgctact ggaatattct tcttaacaaa
    39541 gatcttgaca ccggttttga tattgtattt attttttagc aataaagcag cttgataaac
    39601 attgttccgt tcatctaccg gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc
    39661 tgttatttct tcaaaaaata gatgatctgc caagtctacg ctcgacatca ccatttccaa
    39721 ctcatgatac ccatcttgcc gtctatataa tacatccagt cctaaattta tcttggctgg
    39781 cgctttttca atcaattcca tcctctcacc cattcgaaaa gtctaagtcg caagtattct
    39841 atcataggga aaaataaaag tatagcttat gtctgcctag tggttgattt tgtcatcgtt
    39901 tcaacagctt tcacagaatt ttctcttcat aatccaaaca agttctagtg attcttataa
    39961 ttctgtgagc acttcttctg ccaaactcgg aacatttcct ttttcttttc ctatcagact
    40021 gagtagctgc atggtagcaa atccgaaatt acttcctttc ctataagcaa aataacgtcc
    40081 ttcccaatga tcgacatatt ttaatttctt ttcataagca gactggaagg aatagaaaaa
    40141 attaccataa ttatatacga tatgcatgac tttttcttga aagaaagaaa aatctgtttc
    40201 tccaacatta gctaatggag ctaagcctag attggcttcc gagtagccca ttttctcgta
    40261 tacaataaac agattggcta acagatagtc agctaattcc tcagattctt taggtaagac
    40321 ccgcaacaaa tcatatcccg cctgcttctt cgtcatcggc tgagcggtga tgaagccaac
    40381 gactttttga tctttacgga caataccgac accgctagat aatacatatg atttatcaaa
    40441 tcgtcccaca gagaagtatt tttctcgtgc accttgaagc catttttgtg aaacttcatt
    40501 tacagcttga tgtaattctt ccggcagttg atcgtaccaa gtaaaatggt atccttcttt
    40561 catcaaacgt tgatattcaa gttgctgact cagccgagac aagtcttctg ttgctagatc
    40621 aacaatccct gcttcaccta ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac
    40681 gtagctttct gatatcttat aaaacgcaag ctgatagcct aaacaatctg cttgtttcat
    40741 aaactgattc gttgctgccc gcatcatttt aggattgcca actggatctc ctaaaacaaa
    40801 gcatttgttt cctttgatct gataaccaaa aacgacttga tcttggtcgt cttcttggta
    40861 ataaaaataa ctgtaacctg acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt
    40921 ttccaataaa cggtcaaaac ggtctttttc gtactcactg ccaagcttcg tagtatcttc
    40981 agctaaatat tgataaagcg ccactaaagc tagcaatgag atgcccaaac caataagccc
    41041 tgagaaccac acatcatctg aaggaaacaa aataaaatga tttcctgtta ttcgaccgac
    41101 taaaaagcgt ccgctgtgat agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa
    41161 gctagcatca aagattatag ctccccaaga ataaacgaat tttgttcgat aaaattcttt
    41221 tctggataac cacacagcta acagaatcaa gacatatacc aatatcaact gccaagataa
    41281 cgtacgtgtg attgtattta aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc
    41341 ctttttgact tttactgaaa gtgctctagc aaggccgagt aataaaaagc caaccaatag
    41401 attcactgtc tgatctaaaa aattgaatga gaacggcaat aatacttgaa atagccgact
    41461 gattgcagac agattagtga ttgttgatag aagtaccatc atgatccccg caaaatagac
    41521 agcacttacg agcacgatat gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa
    41581 aaatcgattg attttgactc ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg
    41641 tagaacataa taaaacaacc gataatataa tagccaaact actgctacat cttgccgaac
    41701 agacaattga ctcattccta agatcatcaa aacgtcaaaa gtccccattc cacctgggac
    41761 catcgtcagc attcctatca atgtagcaat catgaacata ggatagatcg aaagcatcga
    41821 aatatctatt ctcatcaact tgccgatgat caaaaatacc gcaagggctc ccgtccattg
    41881 tccaaaggat gccagtagca gaccaagcaa gccttttgga taaaactcga agaacaatgt
    41941 ctttctcttt agataagcaa aaaagaaaat gactggtgct aaaaaactac cgccgaaaag
    42001 ccagatccaa tagcttcgaa aaatactttc tgactgaata aaaaagacat caataaatgt
    42061 aagaaacgac catacagaca aaccagtcag cataaatagt gcgacttttg atacagtggc
    42121 taaaaccatt tttctatttg tctgctttcc ataaaaacta gcccgcagac ttgctcctac
    42181 cacaccacca aaaccggcga gattgttgat cgtgtttgtt acccacgcag caagaagaaa
    42241 ctcttttctt tccatttttg gttttccttg tttttctaag actctgatcg tcacccaatc
    42301 atacaacaac atcggagtga cacccagcag tccagccaga atcatgagca ggatcgtcat
    42361 gcggctttgt tgtcccatcc tttgccaaat atcttgccaa ctcatgccat tagcaatatt
    42421 tgcgacttga tttgccacaa agactaacac ggagccgaaa aaaatcagtt ttaaaatcag
    42481 actatgttcc tttatccatt gcagcatcaa gtatcgttta tttttcacat tttcccattc
    42541 ctaacttgta tacttttgtg tcatacccca atgttatctt atttatttta atttttccac
    42601 tttagacctg ttctttgctt gcgatgatag aaaaagaaag atatcctaat gctaaaggag
    42661 aggataacat gaaagtagga tttatcggca cagggaatat ggcacaagct attattgatg
    42721 gtttattaaa aaaaggaaca gtctcgaaag aagaggtgtt cgtttccagt ggacattttg
    42781 aaaatgcaca aaaatttgct gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag
    42841 ttgcatcaca atcagatatc gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat
    42901 tggaagaact aagagaatat agccagaaag tcttattcat ttctattgct tcaggaaaat
    42961 cattaataga tttagaaaca gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc
    43021 cgaatgtcaa tgtcagcgta ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca
    43081 cggaggaaca gcttcagcaa gctacgatga tttttgaagc aatcggcagc gtctatcctt
    43141 tagcagaatc agatttcagt acattcattg gccttgctgg cagctcaccc gcattcgttt
    43201 acatgctgat cgatgcgatg ggacggactg gtgtactgaa tggtttgtcc aaacagacag
    43261 ccactgagat tgctgcaaaa gcaattatgg gttcttgtca aaaattcctt tcaagtgata
    43321 aaaatccatg ggaacttgta gaccaagttt cttctcctgg cggtacaact gttgcaggtg
    43381 tagttgcctt agaagaagca ggattcatcc cagcaatcat caaaggaatc aatacgacga
    43441 tcgagaaaga tcttgagatg ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt
    43501 acaaaaaaag aagagctgtg gcttttgccg cggctcttct tttgtatttt ttattattta
    43561 gcataatcag ttgcacgagt ttcccgaata acgattactt tgatgtgtcc aggataatcc
    43621 agttcttctt cgattcgttt acgaatatca cgtactaaac gaacagcgtc taaatccgag
    43681 atttcttctg gcttgaccat tacccgaact tcgcgtccag cttgaactgc gtaacttgat
    43741 tctacaccgg cgaagctgtt cgaaatattt tccagacttc ttaaacgacg gatgtagttt
    43801 tctaatgatt cacttcgcgc accaggtctt gctgctgaga gcgcatctgc tgcagctaca
    43861 aggacagaga taacagaagt agcttccaca tctccatgat gagaagcgat cgcgttgatt
    43921 actactgggt tttctttgta cttggcagct aattccgcac cgatctcaac gtgagatcct
    43981 tcaatctcat ggtcaagtgc tttcccaata tcatggagca acccggcacg ttttgctaat
    44041 tggatatctt cgccaagctc tgctgctaag ataccagcta attttgcaac ctcgaccgaa
    44101 tgcttcaaga cgttttgtcc ataactagtt ctgaatcgca gacgtccgat gatcttgatt
    44161 agatctggat gaagtgtatg agcaccgact tcaaacgctg cctcttcccc gtattcgcga
    44221 atacgttcat ccatttcttt acgagatttc tcgaccattt cttcgatacg cgctggatgg
    44281 attcgtccat cttgtatcag tttttccaag gtcatccgtg cgatttctct tcggattgga
    44341 tcgaatccgg aaagaacaac tgcttccggt gtatcatcga tgattaaatc gataccagtc
    44401 agcgtctcaa gcgttcgaat attacgacct tctcgtccaa tgatacgacc tttcatttca
    44461 tcattcggaa gggtaacgac tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt
    44521 tgaatcgcta atgataataa gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt
    44581 tcagattctt ttaccattag agttaactca tgattcagct cttcttcagt cgatttcatg
    44641 atgacatctc gtgcgtcctc ttttgataaa ccagcaattt tttctagttc tttttgttga
    44701 tcttcaatca ttttttcaac gtctttttct cgctcttcaa ttaattgctg ttttgaacta
    44761 agtgcttctt ctttactttc aagtgagttc tcgcgttttt ctaacgagtc atctttacga
    44821 ttcagcgttt gttcgcgttg aatcaaacga ttctcttgag attttaattc taacttactt
    44881 tctctcagct cactttcaac ttcagaacgg tatttctgat tttcttcctt agcttctaac
    44941 aatgcttctt tttttagagt ttctgcttct ttttttgcac tatcgatgat tccagcagct
    45001 gatgattgtg caccagctat ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa
    45061 ccaagaccga caattaaacc gatgatagcg aggagaatgt tgaataccat aaaatccacc
    45121 tccatactat ctttttcatt tttatcatct atacagcttc tatgataaat tattctaaac
    45181 tcgtcaatat gcatcttcgc atatatgtgt acaaaaaaca tgcacaactt tattctaatg
    45241 tttgtacccc gtagtgtcaa ctttaaataa acaaagaaac ggctttctac ttattcatta
    45301 aacgatggtt tttaaaataa atgacactag tttcatatag gtaaaccttt aaattgagaa
    45361 acttttctat taaattattc tttgtagatg aacagatatt tttattttat gtacccactc
    45421 gtataattaa ataactcacg atttctttat tcaataaaaa aacggcaggt caaggagagt
    45481 acctactctt cagaccagcc gtttttgttt atcataattt ttattcttct actaaaggca
    45541 attcttcttg tcctttttca tcttctggga cagcgacttc ttcaccaata ccataagctg
    45601 ctctaactag agcagaaact tctgccatca tttcaggatg attagccata tagattttag
    45661 cgttttcccg tccttgacca atccgatctt ctttataaga ataccaagca ccgcttttat
    45721 caacaatgtc tttttctact gccatgtcta gaagttctcc ttcttgtgag atccctaaac
    45781 catacatgac atctacttca gcgattttga aaggaggtgc tactttgttt tttacgactt
    45841 tgatctttgt acggttacca acaatatctg taccttgttt caattgttct gcacgacgta
    45901 cttccaaacg gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg atttctggat
    45961 tcccaaacat cacgcctact ttttcacgga tttggttgat gaaaatagcg attgtttttg
    46021 ttttgttgat cgaaccagag agtttacgca atgcttgaga catcaaacgt gcttgtaacc
    46081 cgacatgtga gtcacccatt tcgccgtcga tttctgctcg tggaactaaa gcagcaactg
    46141 aatcgactac tacgatatct acagccccac ttgatactaa agcatcagcg atctctagac
    46201 cttgttctcc tgtgtcaggc tgtgaaagaa gtagttcatc gatattcaca cctaattttt
    46261 gtgcatattg cggatctaac gcatgctcag catcaatgaa agcggccgtt ccgccatttt
    46321 tttgtacttc tgcaatagcg tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat
    46381 atacttcgat gatacgtcca cgggggtaac cgccgacacc taaagcgaca tctaacgcaa
    46441 gagagccact tggaattgta gagatttgtt ggtcgatttt ttcgcccaat ttcataatag
    46501 agcctttacc atagctcttt tcaatttttt tcagtgcagc atctaaggct gctttacgat
    46561 catctgccaa tacgataatc ctccttatat ttacaaatca gagcaacatt tcattatcct
    46621 tatcatatct tttttagtga aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc
    46681 attttcttaa aagctgtttt ctcaaaagat caaatccttg cataaccgca ttatatctga
    46741 tagcagcacg atctctggca aagtgatatt tttccgaaac cacgccgtct ttagatgaaa
    46801 tagcgatcca tactgttcct gcagattgac cttctaacgt gtccggtcca gccactccgg
    46861 tgaaagctac actgtaatct gttcttgcta tctcacgtgc ttgaatggcc atctgctcca
    46921 cacattcttt gctgactgtt ccatattctg ctaatagctc cggatcaatc tccagaaaac
    46981 cagcttttgt ttggagcgag taggtaacga atccgcctgg aaatacttca gatacaccag
    47041 caatgtcgcc taatgcggct tggaaggcgc cggctgtcaa actttccgct gctgttacag
    47101 tctgcttgtt ttctttcagt agttccacta cgacttttgc tagcgaatta tcatcgccgt
    47161 atccataaaa atattcccct actcgttctt ggattttttc ttctaatgct aaaagagctt
    47221 gattccctgc ctgaacatca tttgttttca ctgtcagtcg taatgtcact tcatttggtt
    47281 tggcatatgg tgcgatcgtt ggattgatct gtgtctcaat cagatctttt agctcagtga
    47341 ccagtcgcga ttcaccaatc ccataaaagc gcaaaacttt agaaattagt ttttcttcag
    47401 aaggaaaacg ttcctctagt aaaggacgta cttgttccac aaacattggt ttcaactcat
    47461 taggcggacc gggaagaagg atatacgcat gtgtatctgt ttgataaaaa gtccctaaag
    47521 caagccctgt acgatttggt agaggaaccc cgccttcgat aatctgggac tgttgcagat
    47581 tgtttttggt catttttcga tgggttgttt caaaatatgc caataatttt ttgtatcctt
    47641 ccgtattttg tatgagagac ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat
    47701 cttctgtcgg tcctaatccg ccacataaga gtatgagttc acttcttgtt tcagctagag
    47761 caatcaactc ttctaaacgc tgctgattat ctcctacaac tgtttgataa taaacttcga
    47821 tacctagatc agctagctgt tctgacaaga aagtggcatt cgtattcacg acttgtccta
    47881 gtaaaagttc tgttccgaca gctattattt ctgctttcat tacttcccgc tcccttatat
    47941 agtatacgct gtatttttat atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa
    48001 atcttgtcac agctccttgt tccaaataaa cagtgttctc ctgccaaaac ctcggtcata
    48061 agtcacaata tacataaaac atgaaaagct gttttttgaa attatccctt gtgactcaga
    48121 gttagacggc aggctcacaa cctctccact aaacgatact tacattgatc ctttgaacac
    48181 accgctgttt ttgatgaagt aatccacacc tgaatagata gtaaagatca gacaagcata
    48241 aagcatgatt tgatctactg gaataccgat caggctgaat gggatattat tgataaacag
    48301 caagataata gcaaccattt gtgtagccgt cttgactttt cccggccaag ctgcagccat
    48361 gacttctcca tgttcgacaa gtagtaaacg caagcctgtg acggctaatt cacgacagac
    48421 aatgatcgct actacccaac caggggcctt tccttggtca accaacatga taaaagcagt
    48481 cataactagc attttatctg ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc
    48541 tcgcgcacgg gcaatttttc catctaacca gtctgtgata ctagctaaag caaaaataac
    48601 tgcacctact agctgtgtga cagccagtgt ggtgtctcct acgttaaatt gtccccaatc
    48661 taaaggtaaa cctacaacca aaatgaaaat cgggatcata caaattctaa gtacagttaa
    48721 tttatttggt aaattcacac tatcgactcc tttcttccct attctacgtg actatcagcg
    48781 aagtttccac cgatattatt tacttttcca aatgaattta ttcaaaggag tatttttatt
    48841 gaaattgatc tttttgaatt aacgaagaag gaccgcgaca cagatttgtc acgatccttc
    48901 ttttttatac ctacaagtaa acagatgcgt aaactaattt atacgatcat tctgcgtatt
    48961 gaagagtaag gttcacattt tttggactct gattgttttc ccctggatta actggtacgg
    49021 attctccatt cgctttgatc gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg
    49081 ttgctgtttc cggtaacacg gtactctgtg tttcatttgc tgccagtgtc ccttgataaa
    49141 cataggcatt gtctactagc acacctaacc atacacgatt gacagcagta aattcaaggg
    49201 ttacaggctt ggtagcgtct gtgatattta ctgcgatggc actgccagaa tcttctcctg
    49261 tagctattgc cattttcttt tcttcacttg attttgttgt tgattctact gtagaagact
    49321 ctttggtctg cgtgctttgc tctgaggaag aggactcctc gactgatcct tctactttaa
    49381 gcgaagaagc tgtgtctccg attattggat cagcttgacg atcttgccaa gtcatatagc
    49441 ccacgacaac gacaatcgcc aaagcaacca atcctaataa aatgacagga agacttgtcc
    49501 aaaagcgact tggatgcgat tcttcctgat gcattgcttt tctagaagta cttactgttt
    49561 caggctgcgg acgttcaggg accgatcctg tcaatttgct tttcccatca aaaacatcga
    49621 ctaaatggtc tccatcttct cctacagctt cagcgtattg ccgaataaat gcccgcacgt
    49681 aaaatttacc aggcaattgg tcaaaatcat cattgtcgat tgcttccaaa taacgttttt
    49741 gaatttttgt aatctgctgt aattcatcta aagatatttt tttattcagg cgtgcttgcc
    49801 gtaatttttc tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc
    49861 tccgaagcat ctgctgttgc aacgaaagac gttatgtttg ggtgctgtga caaaagtcat
    49921 gtcacagctc cttttttccg aataaacatt gttctcctgc caactcctcg gtcacaagtc
    49981 acaatatcca ataaatataa gaagctgttt tttgaacttg ttccttgtga ctcggagtta
    50041 aacggcaggc tcacgacctc tttcttaaac ggtgttcaat cagctgttcc tcggtattag
    50101 ctcaaaaacc ggcaaaatat aaggagatat ttttccgtat gtttctctaa tactcaggtc
    50161 aaaacgctga tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
    50221 taagtccaac caagttaaaa acattaagaa gctgttttta cttggctgtt cttattactt
    50281 gatgcagggc agctttttca caacctcttg gtacacggtg ttccagaagt agcttcttcg
    50341 gcaataagcc gaaatatcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    50401 cttgaagctg accacttctg tcacaacctc tttgattact tcgtttgatt ttattttaat
    50461 ataagttgat taaaagagag catgatttgt tttattttct cactaatttt tccaacacaa
    50521 ctaagaatac cataaaagct gattttggag gaggttccta ctacaagtct aaaacgaaat
    50581 ttaagacttt tcctcataaa atactggttt attttggtct catgtaaaac tcgctgaagg
    50641 cctcttcact tataaatgct tccccagctg ccagtacgtc tttcatttgg atcgaatcga
    50701 tgatttcagg aagatcaaat aaagttcgat cgccaaataa actttgcgta aattgattgg
    50761 caatatattc gatcgaattt aatgattgga aatattgtcc aagcattttc ttcttcagca
    50821 aatcgagatt tgtttctgac acttccggat catcagcgaa tccaagaatg atttctttga
    50881 ctttttctgc tgctttctct ggttcgtccg tatctccgct gaagtccgca aagtgaaatt
    50941 cacgatccaa gctgaattca aaaccaaagc tgtcatcgat aattccttga ttgtacatcg
    51001 ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa caacagattg attgctgttt
    51061 tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc taatccttta ataccaagta
    51121 caaatttatc tcgtgtaatg gctgcttcca atgcactttg tttgatgatt tcttttgtgt
    51181 tttcagggaa ataacgtaca atttcttgct ttggagggaa attttttgct tcttggtttt
    51241 cccgaataag tttcatcaat ttctcaggct ccatcttacc aactacaaat aaaaccatat
    51301 tgcttggctg ataaaacgtc cgatagcacg tatacaagtc ttgagctgtg attttgtcga
    51361 tactttcaac cgtgcccgca atatcgatat gcaatgggtg tgtaggatac aagttattta
    51421 gtatgccaaa aaacatccgc cagttagggt cgtcttcata catttggatt tcctgaccga
    51481 tgattccttt ttccttgttt acagtttctt ctgtgaagta aggtgcttgt acaaaatcga
    51541 tcagagtcgt cagattcttt tctacctgat ccgtcgtaga aaacagatag ctggttttcg
    51601 taaaacttgt gaaagcattg gcggaagctc cttgtttccc aaataattga aagacatcac
    51661 catcttcttt ttcaaacaac ttatgttcta aaaaatgggc aattccatct ggaactttca
    51721 ccttttcttt ttcaccatag ggaataaatt cgttatcgat cgaaccataa ttcgtactga
    51781 acaaaccata tgtcttgtga tagtcatttt taggcaacaa atacacggtc aaaccgtttg
    51841 gcagtacttc atgataaagt gtttcattga tttgctcata ttctgtttta ttcatttgca
    51901 tttcctcctg ctaaaaagaa gattgcctgc aattcgattt gctcagctac ttgttgtatc
    51961 tctttgatcg ttacgctctc catacgtttc aaccattctt catcagataa ttttgtttcc
    52021 ggcagccagc tgtccaaaaa agaagcttcg attgccgctt gcggagagtc tagagaaagc
    52081 agatattggt ttctcagcat tgcttttgtc tgagacagtt ccaactctgt tatttcacca
    52141 tttcgcaaac tttctaattg ctcgtgaatc agatgaagta cttgatttcg gttcttttca
    52201 tcgataccgg tttggacact catgaatcct cggaacgtat ccacgctact agaggcataa
    52261 taagctagac tttctttctc tctgacattc atgaaaagtt tagagtgagg aaaaccgcca
    52321 aataaaccgt taaagaccat tagcgcaaaa cgttctggtt catcgtaata cacatttgtc
    52381 tgataagcta ggttcagctt tgcttgaacg attggctcac gtacttgtcg ttccttcacg
    52441 atattcactt gaggttgggt ataaaacaga tctggataaa ctgtttcccg cgactcaaat
    52501 ggtaattgcc caactgcttc agctgctttg tccggatcaa catcgccgac aacaaaaatg
    52561 tcgatttgat catttgccat catttcttga tagtaggacg ctagcgaact agcagtgatc
    52621 ttgtccaaat cttcgactac gcccaaccct gggatctttt gttccgggct gttttcaaaa
    52681 taaagttctt gcaaggcata ggaagcaaaa gtttgtttat cttccttcaa actttccaaa
    52741 tatagacgca gattgttctt ttccaagtca aacgtcaact gatcaaactg atgattttta
    52801 atatttggat aaaataaaac ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga
    52861 tcattgacat attcaccatt cacaaaactg atccctgcgt ttagccaatg taaatttcct
    52921 ttttttctaa ctgataatcc aaagcttgcg ccataaagct ctgcaagttg cgcacttaac
    52981 tttgtctgat ccgaataatt caaactattt gtttccaaca tgctgctgag aagtgaacgt
    53041 ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga taaaaatccg gatcgttttg
    53101 tattgttttg ttggtaagac atgaagattc acgccttctg ccaattgata caccatattt
    53161 atatgctcct tttcacagtt gattgcccgc ttactctttt aatgggtcaa cctgcctttt
    53221 ttacttattc ctctgcttga aatttataag ttcaggcaat tcgccgtttt ttctaaatga
    53281 tgttacactt tatagtatag catagatgtt ttttatcaaa agaaacagac ggctaaaagc
    53341 gtatattgtt tttttatttt ttatttttcg atatactaaa gacaaataaa agaagggagg
    53401 gtcattcatg atcaaagaat tcaaagagtt catcatgcgt ggagatgtct tggatcttgc
    53461 tgtcggcgtt gttattggta gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt
    53521 gatcacacca ctaataggtt ggtttgtcgc aatgatcaca ggtacttctg atttagaagg
    53581 agcactttca attcttgact ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt
    53641 tattagtgct atcattacat ttctgatcac tggttttgtc ttattcttag ttgtcaaagc
    53701 ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa gtggaagtgg aagaaactcc
    53761 tactgcagaa gattatctta gtgacatacg cgatcttctt gccaaacaag tggcagaaga
    53821 caatacacat ccagtagagc aaacaaatct ttccgatact tctaaataat aaagaatcta
    53881 aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac
    53941 ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc
    54001 cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg
    54061 tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa
    54121 accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc
    54181 ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca
    54241 agcttctcca tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac
    54301 tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt
    54361 tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc
    54421 acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta aactgcttca
    54481 gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta agatactcgt
    54541 tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata ggtttctgtt
    54601 aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa catgattggg
    54661 ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg catacagttc
    54721 acctctacta tcaactatac cacatatttt taaaattttc aagtttacca taaaaaaaaa
    54781 agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta ttcctcatta
    54841 taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct cattacttgc
    54901 ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg catataataa
    54961 tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat tgcttctttt
    55021 aataaaagat gctgaattgc taaaggttct tgcaatgtga tctctgctaa aaagtctggt
    55081 ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg acgcttcagc
    55141 gcgttctgct tcctcaacag agaatagatt tgattgctta taacgctttt ataataactt
    55201 ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa agcgatcctt
    55261 gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt tttcaaacgt
    55321 agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc accattacgt
    55381 gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt ttatggagga
    55441 atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat tttttctcat
    55501 taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg gagaattacg
    55561 tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt ccgttttttt
    55621 tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa agatcatcca
    55681 ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat tcagctgacc
    55741 agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa
    55801 ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga agacaacgat
    55861 gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag cttctcttgc
    55921 atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac caatcatgtt
    55981 gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac gttgggaaaa
    56041 agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga cgtgcccggt
    56101 catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca ttcctttgcc
    56161 ttcattacca atgactaaag caattgatcc tttggcattc catttttgga agtttgtgcc
    56221 ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca cagcttgtac
    56281 caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag tctttgttac
    56341 gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga cgccacttgc
    56401 gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa
    56461 aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa gggataaata
    56521 ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc
    56581 taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga tttctttcag
    56641 ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg
    56701 ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta atgcttcttc
    56761 attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat atctattttg
    56821 ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt tacccatttt
    56881 tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg gtttgttttg
    56941 tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac gtcgtaacat
    57001 cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa taaagttctt
    57061 cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct tgtgcattcg
    57121 cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct ccttgatcaa
    57181 tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc aaggctaaac
    57241 cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt gcggtgtatc
    57301 ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc
    57361 acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt catctaataa
    57421 ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata aagcttctcc
    57481 ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca gccacttagc
    57541 aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat
    57601 gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg ccaaattctc
    57661 tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt
    57721 ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt attgtgtggt
    57781 cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat gtacagtgat
    57841 gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta catagccatt
    57901 gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg cgatttcatt
    57961 ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg tttctcctaa
    58021 atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt tgccccatgg
    58081 tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat ctaatgggtc
    58141 ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca gttcatcgat
    58201 ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat acacatcacc
    58261 tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta aaatatctgg
    58321 catatgatcc atgactcttg gatgcagtgt agcaggttcg acattcaacg ctttcgtatc
    58381 ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac ctaattcgtt
    58441 tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat cgactttata
    58501 gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac gagcatttcc
    58561 gatatggata taattataca cagtcggtcc gcagacatac ataccgacat gtcccttttg
    58621 tatagggatg aattcttctt tttttctggt caatgtattg tagattttta tcattctgat
    58681 ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac cgctgtagcg
    58741 tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt ttttccgatc
    58801 gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt aacagttggg
    58861 tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa taaaatcaca
    58921 tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc gatgaacacg
    58981 ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg ccagaattga
    59041 gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga gaaacgatgc
    59101 caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga acgtgcagcc
    59161 ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac tttccactcc
    59221 tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa
    59281 taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat aatcaatgat
    59341 tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct ataaaacagt
    59401 ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc aaccattcat
    59461 tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact tttttctgtt
    59521 catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg accaagcaat
    59581 tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac acggattggc
    59641 atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat ggcagctttg
    59701 acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc gtggagaaca
    59761 gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac aggatgttca
    59821 ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg aggttgatac
    59881 aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc tgctacacgt
    59941 ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc cattgctttg
    60001 atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg tgatttactt
    60061 aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc
    60121 acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag atagcctaat
    60181 tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag ttttttacct
    60241 gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct
    60301 tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg taaaacgtga
    60361 gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg cataccatcg
    60421 cgtttttgga tgacaaagtc gccaccgata ttatcggatt cgaacgaaat agcgcctttc
    60481 accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac aacagattct
    60541 agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg
    60601 taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct
    60661 gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag cggttgatag
    60721 atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc aggagattca
    60781 tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc atcttcgata
    60841 ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt gtggcgggca
    60901 aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc agttggactt
    60961 ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac tagactttga
    61021 ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta aagccactca
    61081 gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat ttccttacca
    61141 gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct cttttttatt
    61201 gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag cagctgtttg
    61261 aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc catcttctac
    61321 gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg tcccggcttt
    61381 tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg catttgccaa
    61441 cgcattgata ttaagaacag gtacattttg gaattcagag actttattca agttatagtc
    61501 attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc tgtctacttc
    61561 actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt ctttttgcaa
    61621 ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat cccctgagtc
    61681 cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc cttctaagaa
    61741 tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat ccaagatttt
    61801 atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa cttgactagc
    61861 tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt cttcgattcg
    61921 tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa tcggcaatac
    61981 actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa cacctaaagt
    62041 caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga cctcacttag
    62101 tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca aagataataa
    62161 aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc atccgtttga
    62221 ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc ttgcgccgac
    62281 aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc
    62341 atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta ctccaactat
    62401 ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt atacttttgt
    62461 gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac gtctgatttc
    62521 tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt tttctttata
    62581 gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta attttacccc
    62641 acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc ttttttccag
    62701 cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc gtttggcatt
    62761 tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac gtgtcccttc
    62821 cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta agaaaatctg
    62881 tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt
    62941 cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat gttgttcacc
    63001 ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg cgatcgagcc
    63061 ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag cggttttgag
    63121 tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg tgatgtctgg
    63181 ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat gttcgatggc
    63241 acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt tgatacttcc
    63301 gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata catataagac
    63361 ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg atttcccgat
    63421 acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc ctaacacacg
    63481 gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga cgtctgcgat
    63541 ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc
    63601 ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa
    63661 cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt tttttgccat
    63721 tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt atctttaacg
    63781 ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg atcttgatct
    63841 gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt gatcactaca
    63901 tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc ctcattattg
    63961 taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg cttcaacgga
    64021 ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca
    64081 gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt tttcaatcca
    64141 tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg aatcacgacc
    64201 tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg cggtagattg
    64261 atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac cggttttcct
    64321 ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa
    64381 ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac
    64441 tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata
    64501 gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat
    64561 gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc
    64621 aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag
    64681 gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca
    64741 gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg
    64801 atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg
    64861 acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag
    64921 cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa
    64981 ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt
    65041 gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg
    65101 actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg
    65161 gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa
    65221 gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg
    65281 gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg
    65341 aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac
    65401 ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga
    65461 atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata
    65521 tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta
    65581 cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga
    65641 cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg atcggcatca
    65701 tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga
    65761 atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat
    65821 tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg
    65881 atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga
    65941 ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca
    66001 ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg
    66061 cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa
    66121 cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg
    66181 tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg
    66241 ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag
    66301 tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag
    66361 aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg
    66421 cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg
    66481 cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat
    66541 taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt
    66601 gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg
    66661 tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg
    66721 cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga
    66781 gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac
    66841 gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt
    66901 ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat
    66961 cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg
    67021 tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg
    67081 acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg
    67141 gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat
    67201 aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt
    67261 cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt
    67321 ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca
    67381 accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat
    67441 attcttccag catctattat ccccccattc tttttcttaa atgaatttta gcttacctac
    67501 tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca
    67561 catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat
    67621 attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga
    67681 cagtctcaca acctcttggt aaacggcgtt caatcagctg accctcggta ttagctcaaa
    67741 aaacggtaaa atatgagaag ctatttttcc gttttgtttc tctaatactt aggataaaac
    67801 gctgatttta caacctctgt taaacggtgt tcaatcagct gtccttcggt attagctcaa
    67861 aaaacggcaa aatatgagga actatttttc cgtttgtttc tctaatactc aggacaaaac
    67921 gctgatttta caacctcctg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    67981 caaccaaacg aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag
    68041 gacagctttt tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg
    68101 gtgataaatg atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata
    68161 gctttcacca atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa
    68221 aaagcggaca aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat
    68281 agcaatcgtc gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct
    68341 gatcggtaca aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa
    68401 taagacaaat gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc
    68461 aacgaaaatc gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc
    68521 taattcagaa tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa
    68581 attatataac aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac
    68641 accttcccaa aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag
    68701 ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc
    68761 actgcccaag accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc
    68821 atacatcatg attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc
    68881 agtagagaca aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc
    68941 gatattccca gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat
    69001 caatgttttt ttcaaaccga tccgactaga taagaaacct gtaaatagca tcaccacacc
    69061 agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag cgtttaactg
    69121 tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag cgatcataca
    69181 ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg tttgttcttg
    69241 tatatctatt tgattctcca atatttccat ccctttctct ctattagcat catacatgat
    69301 ttattttaca tgtcaatatg cacatgtaaa acgtggaaac tttactgtga tataataaag
    69361 aaaataagac gagaggtagg atttttatga atactttagc ctatatatta ttatgcgtgc
    69421 ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt ttttgggaag
    69481 cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt tatgtgaccg
    69541 ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat gaaggacgtg
    69601 ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc
    69661 tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga ctgctattca
    69721 ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct
    69781 cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga attatgcttt
    69841 gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag ttttttgagt
    69901 aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc ttcaataaaa
    69961 gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg
    70021 atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc caaggccttt
    70081 tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac acttcttgaa
    70141 agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat tttccaagca
    70201 ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac accgaataaa
    70261 gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat catttggacc
    70321 ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac accgatgatg
    70381 aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa caaacttgat
    70441 aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac catcaaaaaa
    70501 cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct gtaccactcc
    70561 tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa gtactttttt
    70621 tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa agtaagaatc
    70681 atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta aagcttcgat
    70741 cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac ttggcttagt
    70801 aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac ataacggttt
    70861 cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa gcgattctga
    70921 aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca tttgataacc
    70981 cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt ctccttgttt
    71041 gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt
    71101 tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac ttctttccaa
    71161 ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt ttggtactgg
    71221 aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt ggagtcttct
    71281 ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt gtttagagga
    71341 agacaaccga tccagtctga tttgttgctt tttcttttta ctggacactt tttccaactt
    71401 ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct ctttcatgat
    71461 cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga gataggcact
    71521 ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt tcgatgctag
    71581 ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat aataacgaag
    71641 ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct cgtctagaag
    71701 catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt cttcccctcc
    71761 gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg ctaattccaa
    71821 atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga aggtgatctc
    71881 tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga gcaaagtcga
    71941 tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga cacgaagaga
    72001 atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta tatttactgc
    72061 taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat cgatactaaa
    72121 aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga
    72181 aataactatg actaaatcac caaatctgtc atagctttca cctattctac tgtcgttcga
    72241 aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg aatttattta
    72301 gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc cgacctcctt
    72361 ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata gtaactgaaa
    72421 accttttatt cgaacatagt gatcttttct ggtgctaaac gaacagactc gtaaccttct
    72481 tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt atccaaacca
    72541 aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc atagccatga
    72601 tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt gactacatcg
    72661 ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa ttgttgatct
    72721 ctgacttctt ttggcatctt ccctgattcg taagcttggt tgtagatttc ttcgccatat
    72781 tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc attttgtctc
    72841 gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc agcgaccaca
    72901 aagcgccatg gtttcatatt gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc
    72961 agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga tcttctacca
    73021 gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt tcgtttctgt
    73081 tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt gtttcatttc
    73141 tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca tttcacaatg
    73201 cttgattttc ttctgggctt tttctgttag cgctacatac acttcccggt tgttctgagg
    73261 atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac gagtaactgc
    73321 tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat tttcttcttt
    73381 cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt caaagatctg
    73441 gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct cttctaaaga
    73501 cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa taatagaata
    73561 aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc aatagaaaag
    73621 tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa agcacaacgc
    73681 taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg cagtagataa
    73741 ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa
    73801 attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg tcacaacctt
    73861 ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac caaacaaaaa
    73921 acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca gctttttcac
    73981 tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca tcggtgttag
    74041 ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt catttggatt
    74101 aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt cataacgcca
    74161 accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat ggtctccact
    74221 gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc aagcaatccc
    74281 ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca atgtatataa
    74341 atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag ttggtgctgt
    74401 atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac cataattagg
    74461 cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt tcgtattgct
    74521 tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt
    74581 tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg cattcaccgt
    74641 aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc cattatagct
    74701 tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt tcccccagcc
    74761 actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg cacttgccaa
    74821 actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag cagatgagcg
    74881 agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt gatgggtatg
    74941 tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct ctggattgtt
    75001 ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg
    75061 ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac tttctgtatc
    75121 gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt ctgtcgattc
    75181 ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa cgctaaataa
    75241 taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc taaataacag
    75301 tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt gtttcttttt
    75361 aataaatttg ctttttctgc ataaaaagac gtaaatgaag aatattcttc tccattcacg
    75421 ccttaaattg atggtcgttt gaataactat ttaacaagta attctctctg gatattatcc
    75481 gttatggatt atacttcctg tgtcgtcgga cggataatca tgttgctgac agacatccgg
    75541 tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcagatgt taatccccat
    75601 tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt ttgatataat
    75661 tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg ccgttgctct
    75721 tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata aacggcggaa
    75781 tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc agatttttgt
    75841 ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat gttgatatca
    75901 agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg catgattcct
    75961 gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc gataactgct
    76021 tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc ttctggtaaa
    76081 gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag tttagcccct
    76141 ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc catgatgaca
    76201 atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa atctcttgct
    76261 tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac aggtttgacg
    76321 attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt aacagttgca
    76381 ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt tgctcctgct
    76441 ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct tcgttctttt
    76501 ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa tactgaatct
    76561 tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa gacatttttc
    76621 cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt caccgtatca
    76681 tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg ttcatggtac
    76741 ccagtattca gcgctgctaa aggctcttcg ttttccgctt ggatctgatg gatcacttca
    76801 aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat gaggcttgtc
    76861 attttgctcc ctccttttat ccttacttta taacggacgg cttatgatgt ctaatactta
    76921 taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt taaaaacagg
    76981 agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag agaaaaaaat
    77041 atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa acaactgaaa
    77101 gatttagaag aagaacttgg tgtgacactt ttcactcgag gctcccgaga gatacagttg
    77161 acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa
    77221 acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat tggcggaggc
    77281 gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa ggcgcatcct
    77341 gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa actggatagc
    77401 ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt
    77461 cgactgccag cccaagatcg ttggggactt ttgatacatc ggaatcatcc tttagcaaag
    77521 caaacagtca tccatccgca ggaactgaaa actgttcctt tgatcatttc ccggcaaacg
    77581 tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt atcgatcgtc
    77641 ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac ttttggtgca
    77701 ttagctctag atgggatcat caatacttat gggacggaaa aagtattcat cccactcgat
    77761 ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct ttctcctgct
    77821 gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt tttttccttc
    77881 gctttcagct ttttaacaac gaataagtta agcaaaagat ccaatgatta aataacataa
    77941 gtttcttaat ttgttccttc cttttatttc tgataattcc agtaaattac tataattata
    78001 aaaaaagaat tgacaaaaca taactgtcta tataaaattg attacgttaa caataattcg
    78061 tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg tttccctagg
    78121 aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc
    78181 aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat
    78241 tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga tttgacagaa
    78301 aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg gaaccagaag
    78361 gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta tttgcagcag
    78421 aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa catgtattga
    78481 tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt atcgtcatcc
    78541 gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt attgctcaat
    78601 gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac gaagcaggat
    78661 atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat cgcagtatcc
    78721 gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg gaaacagata
    78781 tccattcaga accattttca ctgaattatt caagactggt gaatcattta cgactgcttc
    78841 tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta gaaatggtca
    78901 aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag
    78961 aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat atcgagcgtt
    79021 taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa aattgaaagc
    79081 atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac ctgctgctgc
    79141 tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg tcgctcattt
    79201 tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg ccgttgggtt
    79261 agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt
    79321 tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga atatcaaacc
    79381 agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta ttattgccgg
    79441 attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga tggctttatc
    79501 tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt tgctgatcag
    79561 tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt ttgctaaatt
    79621 cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc gtttactgat
    79681 ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag caggaatcaa
    79741 tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc
    79801 tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta tttacagaaa
    79861 tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ttagcagcag cttttgcttc
    79921 attctttact ggtgtaacag aaccacttga attctcattt atgttcgttg cttggccatt
    79981 atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat tcttccattg
    80041 gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc
    80101 gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag tgatttacta
    80161 ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc
    80221 agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg cggcacaagc
    80281 aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg ataactgtac
    80341 tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata aaatcaaagc
    80401 cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac
    80461 agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg caacacgacc
    80521 tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtggaacga tcgaacaaga
    80581 tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag atgatgtatt
    80641 cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag aaatcttctc
    80701 tccaatcgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg gaatccaaac
    80761 agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact taaaaggcga
    80821 accattcact ttatatgtaa aagaaggtca aaaagtcgca agaggtcaat tgattgcttt
    80881 agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg ttgtattcac
    80941 aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga
    81001 taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac ttttttatcg
    81061 ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg aataaaaacg
    81121 gcgttctcct gccaactccg cggtaataag tccagccaaa cgtaacatat gaagagctat
    81181 tttttatttg actcttctta ttacttgatg cagaacagct tttttacaac ctctgataaa
    81241 cggtgttcaa aaaccagttt atcggtatga agtcaaattt atgcaaaaat gtggaaagcc
    81301 attttcacaa aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata
    81361 cattattcct gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc
    81421 aatacttttt ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg
    81481 atcgctaaaa acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca
    81541 aacgctgcat cacttgcttc aataatttct aactttttac ctgtcgcacg ctctaatgcc
    81601 ttggctaatt cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca
    81661 ctttctacta gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt
    81721 tttccatttc ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt
    81781 aattcattct ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta
    81841 atcatttttt cagtgaattg gtgatctggc gctaatacac tgatcgagtg atcggcatct
    81901 gcaaaacttg tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc
    81961 ttatgttcgg cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct
    82021 ttcaacgctt ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca
    82081 gcttctttca gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt
    82141 ggaaccatct tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt
    82201 actaaataat gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta
    82261 catttataaa ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat
    82321 gaaaggatga caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc
    82381 ctatattgaa atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat
    82441 cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat
    82501 ctttacaaaa accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt
    82561 actagatgtc tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac
    82621 aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg
    82681 gctgcagaaa aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa
    82741 aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt
    82801 ataattctct ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta
    82861 ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag
    82921 cgctgaagtc atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc
    82981 tgaaaaatgc aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa
    83041 gcgctacttt cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct
    83101 cccatatatt tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag
    83161 gcgtaatctt ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt
    83221 aattcttcag gcgattctgc taaagcgatt acaacaagat acgttccttt tgggaatggg
    83281 atgattcggg tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt
    83341 ttcccgttat agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat
    83401 gcatctattg ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa
    83461 ccgacaaaat ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct
    83521 ggtttttctt cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca
    83581 tcttacataa aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta
    83641 ttttttaact atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa
    83701 tcctctcccc attttgtaaa agagatctcg ggagcttcga ctaactcata atctgaagaa
    83761 ggaagccatt ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct
    83821 ttattaggaa aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac
    83881 ggattttctt ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag
    83941 cgggctccat caaatccata agaaacgttg attacttgat ttggatataa atctcctaac
    84001 ttatgcatct cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga
    84061 ttgactcctt caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct
    84121 ttcttttcga tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag
    84181 cgtggaaaag atttttgtat gccgtatctg ctgatttctg aaggcagata tcctgaccaa
    84241 tcacgaaaag ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct
    84301 gtcactcttg atccatctag gagatcgcga ttagccaaag ccaatctacg gtttttgata
    84361 tagtccatta gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag
    84421 attcctgtat tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg
    84481 taatcgatca attggttcag atctttaagc atatttatct tcctccttgc ttgccttttt
    84541 atcatattat caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta
    84601 gaaaatgttg tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt
    84661 attatctgcc tatctatgtt ttacctttag aaaaataaat tttggggaag acacattcta
    84721 agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg
    84781 tacaaagcaa attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga
    84841 tacatcatac ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt
    84901 tttgtcagat aaataaaa
//
LOCUS       DAYYDS010000004        81280 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000004 DAYYDS010000000
VERSION     DAYYDS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.004"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..525
                     /locus_tag="ACHA02_000275"
     CDS             <1..525
                     /locus_tag="ACHA02_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8668660.1"
                     /translation="KCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWS
                     SSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMI
                     MGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLT
                     VLGICTFIITVINR"
     gene            complement(765..848)
                     /locus_tag="ACHA02_000276"
     tRNA            complement(765..848)
                     /locus_tag="ACHA02_000276"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(812..814),aa:Leu,seq:gag)
     gene            921..1082
                     /locus_tag="ACHA02_000277"
     CDS             921..1082
                     /locus_tag="ACHA02_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668661.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            1231..2448
                     /locus_tag="ACHA02_000278"
     CDS             1231..2448
                     /locus_tag="ACHA02_000278"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8668662.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2632..3468
                     /locus_tag="ACHA02_000279"
     CDS             2632..3468
                     /locus_tag="ACHA02_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8668663.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3544..4692
                     /gene="nagA"
                     /locus_tag="ACHA02_000280"
     CDS             3544..4692
                     /gene="nagA"
                     /locus_tag="ACHA02_000280"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8668664.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4969..5970
                     /locus_tag="ACHA02_000281"
     CDS             4969..5970
                     /locus_tag="ACHA02_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8668665.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            5989..7008
                     /locus_tag="ACHA02_000282"
     CDS             5989..7008
                     /locus_tag="ACHA02_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8668666.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7110..7826)
                     /locus_tag="ACHA02_000283"
     CDS             complement(7110..7826)
                     /locus_tag="ACHA02_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8668667.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(7795..8580)
                     /locus_tag="ACHA02_000284"
     CDS             complement(7795..8580)
                     /locus_tag="ACHA02_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8668668.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8571..9320)
                     /locus_tag="ACHA02_000285"
     CDS             complement(8571..9320)
                     /locus_tag="ACHA02_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8668669.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9618..10142
                     /locus_tag="ACHA02_000286"
     CDS             9618..10142
                     /locus_tag="ACHA02_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8668670.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10158..11657
                     /locus_tag="ACHA02_000287"
     CDS             10158..11657
                     /locus_tag="ACHA02_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8668671.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11745..12347)
                     /locus_tag="ACHA02_000288"
     CDS             complement(11745..12347)
                     /locus_tag="ACHA02_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8668672.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12451..13407
                     /locus_tag="ACHA02_000289"
     CDS             12451..13407
                     /locus_tag="ACHA02_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8668673.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13712..14668)
                     /locus_tag="ACHA02_000290"
     CDS             complement(13712..14668)
                     /locus_tag="ACHA02_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8668674.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14842..15402
                     /locus_tag="ACHA02_000291"
     CDS             14842..15402
                     /locus_tag="ACHA02_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668675.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15395..16219
                     /locus_tag="ACHA02_000292"
     CDS             15395..16219
                     /locus_tag="ACHA02_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8668676.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16229..16846
                     /locus_tag="ACHA02_000293"
     CDS             16229..16846
                     /locus_tag="ACHA02_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8668677.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16864..17547
                     /locus_tag="ACHA02_000294"
     CDS             16864..17547
                     /locus_tag="ACHA02_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8668678.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18230..19465
                     /locus_tag="ACHA02_000295"
                     /pseudo
     CDS             18230..19465
                     /locus_tag="ACHA02_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            19473..20360
                     /locus_tag="ACHA02_000296"
     CDS             19473..20360
                     /locus_tag="ACHA02_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8668679.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20377..21213
                     /locus_tag="ACHA02_000297"
     CDS             20377..21213
                     /locus_tag="ACHA02_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8668680.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21216..21839
                     /locus_tag="ACHA02_000298"
     CDS             21216..21839
                     /locus_tag="ACHA02_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668681.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21836..23128
                     /locus_tag="ACHA02_000299"
     CDS             21836..23128
                     /locus_tag="ACHA02_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8668682.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23487..23584
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23700..24281
                     /locus_tag="ACHA02_000300"
     CDS             23700..24281
                     /locus_tag="ACHA02_000300"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8668683.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24284..25591
                     /locus_tag="ACHA02_000301"
     CDS             24284..25591
                     /locus_tag="ACHA02_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8668684.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            25884..26372
                     /gene="purE"
                     /locus_tag="ACHA02_000302"
     CDS             25884..26372
                     /gene="purE"
                     /locus_tag="ACHA02_000302"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8668685.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26365..27489
                     /gene="purK"
                     /locus_tag="ACHA02_000303"
     CDS             26365..27489
                     /gene="purK"
                     /locus_tag="ACHA02_000303"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8668686.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27502..28797
                     /gene="purB"
                     /locus_tag="ACHA02_000304"
     CDS             27502..28797
                     /gene="purB"
                     /locus_tag="ACHA02_000304"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8668687.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(28977..29354)
                     /locus_tag="ACHA02_000305"
     CDS             complement(28977..29354)
                     /locus_tag="ACHA02_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668688.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29610..30338
                     /gene="purC"
                     /locus_tag="ACHA02_000306"
     CDS             29610..30338
                     /gene="purC"
                     /locus_tag="ACHA02_000306"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8668689.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30338..30592
                     /gene="purS"
                     /locus_tag="ACHA02_000307"
     CDS             30338..30592
                     /gene="purS"
                     /locus_tag="ACHA02_000307"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8668690.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30594..31265
                     /gene="purQ"
                     /locus_tag="ACHA02_000308"
     CDS             30594..31265
                     /gene="purQ"
                     /locus_tag="ACHA02_000308"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8668691.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31266..33488
                     /gene="purL"
                     /locus_tag="ACHA02_000309"
     CDS             31266..33488
                     /gene="purL"
                     /locus_tag="ACHA02_000309"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8668692.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33473..34912
                     /gene="purF"
                     /locus_tag="ACHA02_000310"
     CDS             33473..34912
                     /gene="purF"
                     /locus_tag="ACHA02_000310"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8668693.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            34935..35987
                     /gene="purM"
                     /locus_tag="ACHA02_000311"
     CDS             34935..35987
                     /gene="purM"
                     /locus_tag="ACHA02_000311"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8668694.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            35984..36562
                     /gene="purN"
                     /locus_tag="ACHA02_000312"
     CDS             35984..36562
                     /gene="purN"
                     /locus_tag="ACHA02_000312"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8668695.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36559..38094
                     /gene="purH"
                     /locus_tag="ACHA02_000313"
     CDS             36559..38094
                     /gene="purH"
                     /locus_tag="ACHA02_000313"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8668696.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38110..39384
                     /gene="purD"
                     /locus_tag="ACHA02_000314"
     CDS             38110..39384
                     /gene="purD"
                     /locus_tag="ACHA02_000314"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8668697.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39506..40420
                     /locus_tag="ACHA02_000315"
     CDS             39506..40420
                     /locus_tag="ACHA02_000315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8668698.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40553..42118)
                     /locus_tag="ACHA02_000316"
     CDS             complement(40553..42118)
                     /locus_tag="ACHA02_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8668699.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42111..43562)
                     /locus_tag="ACHA02_000317"
     CDS             complement(42111..43562)
                     /locus_tag="ACHA02_000317"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8668700.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLSASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43576..44592)
                     /locus_tag="ACHA02_000318"
     CDS             complement(43576..44592)
                     /locus_tag="ACHA02_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8668701.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            44812..45900
                     /gene="chvE"
                     /locus_tag="ACHA02_000319"
     CDS             44812..45900
                     /gene="chvE"
                     /locus_tag="ACHA02_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8668702.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            45954..47480
                     /gene="mmsA"
                     /locus_tag="ACHA02_000320"
     CDS             45954..47480
                     /gene="mmsA"
                     /locus_tag="ACHA02_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8668703.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47482..48687
                     /gene="mmsB"
                     /locus_tag="ACHA02_000321"
     CDS             47482..48687
                     /gene="mmsB"
                     /locus_tag="ACHA02_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8668704.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            48944..49921
                     /gene="guaC"
                     /locus_tag="ACHA02_000322"
     CDS             48944..49921
                     /gene="guaC"
                     /locus_tag="ACHA02_000322"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8668705.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50185..51609
                     /locus_tag="ACHA02_000323"
     CDS             50185..51609
                     /locus_tag="ACHA02_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8668706.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(51651..51908)
                     /locus_tag="ACHA02_000324"
     CDS             complement(51651..51908)
                     /locus_tag="ACHA02_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668707.1"
                     /translation="MPIRILTALVNKITIRKAFYFSSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            52070..53179
                     /gene="pdhA"
                     /locus_tag="ACHA02_000325"
     CDS             52070..53179
                     /gene="pdhA"
                     /locus_tag="ACHA02_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8668708.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53182..54159
                     /locus_tag="ACHA02_000326"
     CDS             53182..54159
                     /locus_tag="ACHA02_000326"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8668709.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54184..55827
                     /locus_tag="ACHA02_000327"
     CDS             54184..55827
                     /locus_tag="ACHA02_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8668710.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            55835..57241
                     /gene="lpdA"
                     /locus_tag="ACHA02_000328"
     CDS             55835..57241
                     /gene="lpdA"
                     /locus_tag="ACHA02_000328"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8668711.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57403..57678
                     /locus_tag="ACHA02_000329"
     CDS             57403..57678
                     /locus_tag="ACHA02_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8668712.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57684..58451
                     /locus_tag="ACHA02_000330"
     CDS             57684..58451
                     /locus_tag="ACHA02_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8668713.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58619..60448
                     /gene="typA"
                     /locus_tag="ACHA02_000331"
     CDS             58619..60448
                     /gene="typA"
                     /locus_tag="ACHA02_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8668714.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60709..62481
                     /gene="recQ"
                     /locus_tag="ACHA02_000332"
     CDS             60709..62481
                     /gene="recQ"
                     /locus_tag="ACHA02_000332"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8668715.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62595..62897
                     /locus_tag="ACHA02_000333"
     CDS             62595..62897
                     /locus_tag="ACHA02_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8668716.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            62900..64063
                     /locus_tag="ACHA02_000334"
     CDS             62900..64063
                     /locus_tag="ACHA02_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8668717.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64089..67517
                     /locus_tag="ACHA02_000335"
     CDS             64089..67517
                     /locus_tag="ACHA02_000335"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8668718.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67538..68662
                     /locus_tag="ACHA02_000336"
     CDS             67538..68662
                     /locus_tag="ACHA02_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8668719.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68643..69089
                     /locus_tag="ACHA02_000337"
     CDS             68643..69089
                     /locus_tag="ACHA02_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8668720.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69073..69411
                     /locus_tag="ACHA02_000338"
     CDS             69073..69411
                     /locus_tag="ACHA02_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8668721.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69530..70090
                     /gene="rsmD"
                     /locus_tag="ACHA02_000339"
     CDS             69530..70090
                     /gene="rsmD"
                     /locus_tag="ACHA02_000339"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8668722.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70087..70578
                     /gene="coaD"
                     /locus_tag="ACHA02_000340"
     CDS             70087..70578
                     /gene="coaD"
                     /locus_tag="ACHA02_000340"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8668723.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70568..71611
                     /locus_tag="ACHA02_000341"
     CDS             70568..71611
                     /locus_tag="ACHA02_000341"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8668724.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            71770..72498
                     /locus_tag="ACHA02_000342"
     CDS             71770..72498
                     /locus_tag="ACHA02_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8668725.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72614..72859
                     /locus_tag="ACHA02_000343"
     CDS             72614..72859
                     /locus_tag="ACHA02_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8668726.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            72856..73074
                     /locus_tag="ACHA02_000344"
     CDS             72856..73074
                     /locus_tag="ACHA02_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8668727.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73210..73749
                     /locus_tag="ACHA02_000345"
     CDS             73210..73749
                     /locus_tag="ACHA02_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8668728.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73742..76015
                     /locus_tag="ACHA02_000346"
     CDS             73742..76015
                     /locus_tag="ACHA02_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8668729.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76053..77099
                     /gene="holA"
                     /locus_tag="ACHA02_000347"
     CDS             76053..77099
                     /gene="holA"
                     /locus_tag="ACHA02_000347"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8668730.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77188..77442)
                     /gene="rpsT"
                     /locus_tag="ACHA02_000348"
     CDS             complement(77188..77442)
                     /gene="rpsT"
                     /locus_tag="ACHA02_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8668731.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(77842..78111)
                     /locus_tag="ACHA02_000349"
     CDS             complement(77842..78111)
                     /locus_tag="ACHA02_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668732.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78373..78501)
                     /locus_tag="ACHA02_000350"
     CDS             complement(78373..78501)
                     /locus_tag="ACHA02_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668733.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(78581..79036)
                     /locus_tag="ACHA02_000351"
     CDS             complement(78581..79036)
                     /locus_tag="ACHA02_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8668734.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79174..81102)
                     /locus_tag="ACHA02_000352"
     CDS             complement(79174..81102)
                     /locus_tag="ACHA02_000352"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8668735.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        26364 a        13976 c        17600 g        23340 t
ORIGIN      
        1 aaatgtcaaa actattatgt gcgtatattg actgcattac cgtttttctt aaacctgatt
       61 atttattttg gtaatacaat gggtcaaagc tttacttacg tcaatggaaa taaaagggca
      121 atgatttgga gtagtagtaa cctgaataat ctctttacag agttaggaac gaaactgtca
      181 cttttttatc caggtacttg gatagctact ttagtagtct tggcattatt actatgttta
      241 attgttggaa tttatttaag ttttgacaat aagaaaactt ctattttttt agttattttg
      301 atgataatgg gattttgttc aaggttaatt atgggatttt ctcccacagt atgggcttcc
      361 ggaatgcgga cgtactatat actttatgtt gttatcgcca tcttagtact aatggcagtt
      421 aaagagctaa tgaaaagcat gagtgtacaa aaaaatgaat ttatgcaatt tggattaacc
      481 gtgcttggta tttgtacctt tataataacg gtgataaaca gataatatac aaaaaatccc
      541 tcgctttagt attaggtgag ggattttact cgtttttcga ttaattgttt taacacttgc
      601 aagtttctac gagtagcttg tttccgacct tcatatactc acctcaggct gtttacatga
      661 ttgtacgttc tttttgtaaa ggatacagtg atatgtatat aatggcataa agaaacgttc
      721 aaacaaaaaa actcgtgaag caaaattaac ttcacgagtt gtagtgcaga tgatgggact
      781 cgaacccaca cgagctaacg ctcacatgac cctcaatcat gcttgtctgc caattccaac
      841 acatctgcgt aataataaca cactaatgat agcgaggttt tacatagttg tcaagctcaa
      901 aaatgggagg acttattaag atggagaaat atgacggaga attttcagga ctgggaatga
      961 tcttaggtgt attgattgga cttgcattcg gacgtttttt gcttggtttt atgctaggaa
     1021 tcatttgtgg aattgcaatg gattgggcag cgaatctatg gaatgactat catgacaatt
     1081 aagttctcaa cgcttgtttg tgttttttta atacttacgt ttttcctaaa taagtggcaa
     1141 aattttctag gatggagtat gatattgaaa tagtaaataa caaaaaattt ggaaagttct
     1201 aactttttga aatgagggat tggctaagtg atgagagaat ttgaaaaatc caacaaatta
     1261 gaaggtgtca gttacgatgt tcgcggtccc gttctagaag aagcggagcg gatgcaagaa
     1321 gagggcatta gtattttaaa attgaacacg ggaaatcccg caccttttgg atttgaagca
     1381 ccaaacgaag tcattcgtga tttgatcatg aatgcccgtg actcagaagg ctactcagat
     1441 tccaagggga tcttttctgc tagaaaagca attgagcaat attgccaact gaaacatttt
     1501 ccgaatgtga cgatcaatga tatctacacc ggcaacggag tgagcgaatt gatcacgatg
     1561 tgtatgcagg ggttgctgga caatggagat gaagtactgg ttccaatgcc ggactatccg
     1621 ttgtggacgg cttctattgc attagctgga ggcacacctg ttcattatat ttgcgatgaa
     1681 gaggcagaat ggtatcctga tattgatgat atcaaatcaa aaataacgag tcggacaaaa
     1741 gctattgtta tcattaatcc taataatcct actggggcac tttatccaaa agaattatta
     1801 gaagaaatcg ttgaagtagc tcgacaaaat aacctgatta tttattccga cgaaatctat
     1861 gatcgcttag tgatggatgg tctagaacat attccgattg ctacattagc gcctgatttg
     1921 ttcgttgtta cgctgaacgg tctatccaaa tctcatcgtg tagcaggatt tcgagtaggc
     1981 tggatggtcc taagtggaga taagtcacac gtcaaaggct atatagaagg gctgaatatg
     2041 ttgtcctcca tgcgtttgtg ttccaatgta ttgtctcagc aaattatcca aaccgcgcta
     2101 ggcggttatc aaagtgtcga tgagctgctc ctgccaggcg gaaggatcta tgaacaaaga
     2161 gaatatatct ataatgcaat caatgatatt cctggtcttt cagccgtaaa accaaaagct
     2221 gctttttata tattcccaaa aattgataca aagcgattca atattttgga tgatgaaaaa
     2281 ttcgttttgg actttttaca tgaacatcat attctacttg tacatggtgg tggatttaat
     2341 tggaaccagc cagatcattt tcgaatcgtt tatcttccta aaatggatga tttgaaacaa
     2401 acagctaaga aaatgagaga attcctagca acctatcgcc aaaaatgatt ttaaaaacca
     2461 aatttttcag taggagggcc acagacaaac acactgaggc tctctttcta gtggagtttt
     2521 cttaaataac taaagtatat tatttagttg caagtctttt cttgaagttt gcgacttcct
     2581 aaagtaaaat acgaatagaa tgcgaatacg catattttgg ggaggatact tatggaaacg
     2641 ttaatcgagt cctttggaac gatcattaaa gagattcgaa aagaacaaaa aatgacgcaa
     2701 aaaatgcttt cgcatgatat ttgttcacaa agtgtattaa gtcggatcga aaataatgag
     2761 gaactgccga atgtattggt gatgatgcag atttgtcaac ggctaggggt aacgattgat
     2821 catgtgatga atctctctcg aaaaactact cggacaaacg ataaattatt tgaactgttg
     2881 gatagttgtt tccaaaaacg tgattatcgg aagttagaac aagtactaaa atcttctgat
     2941 atttctgaaa atctgtatca agcaactgac tttcaacgct attattatta tctcggtgtt
     3001 tgcgaattca cgttgcgtca aaatatctct cagtcccttt attatttgaa agaagcatta
     3061 agctactcta gccaaaaaga aagaatccat gtttctgaca cagaaataca actgattagt
     3121 tgtatcggaa aaatatatgg tgtggcagga aaaacggctg aagcacgcta ctatttgagt
     3181 cgaagcatcc aactgctgca tcaatcagct gatgaacgat caaaaagtga attttcgcaa
     3241 attttttata attatggaaa tttcttattc catcaaaatg aaatcgacga agcgttagaa
     3301 caggtgaatc aaggaatcta ttgggctcaa gagaagaata gctactatta cctcaatgat
     3361 ttatttgtgc ttaaaagctt aatatacaag cgtaaagaag aaccagaaag agcagcattc
     3421 tatgaaggct tagcacaagc tgtgaaacaa atttcaaaaa atttgtaaat ggtgtagacc
     3481 attcttgact tcttctctaa ataagagtac tttagaggta gaaaaacaaa gggagagaat
     3541 agaatgagaa cttttatcta tgctgacaaa ttctttttag cgtcaggtgt caaacatgca
     3601 ggctatcttg aaattaccga tggacttttt ggtgaataca ccaaagaact acctgaggga
     3661 gaagtcaaca tcattgacca aagcggcaaa tggattgctc ctggtttggt agatacacat
     3721 attcacggct atatgaatca tgatgtaatg gataatgatg cagaaggcat caaagtgatg
     3781 tctgaaggtc tgttatcttg tggagtgact tcatttttac caacaacatt gacgtcaagc
     3841 aaagaacgtt taaaagatgt agctgagaca atcggcaaaa tgtatcagga agcaccagga
     3901 gctaaaatca aaggaatcta ttttgaaggt cctttcttca cagaagaaca caaaggggca
     3961 caaaacccaa gttatttcgg ggatcctgat ttggatactt tccatgaatg gcaagaagct
     4021 tcaggtggtt taatcaaaaa aattgcttta gcaccggaac gtcaaggtgt agaagagttt
     4081 gtcaaacaag tgactgaaga aggagtagtt gtttctctag gacacagtaa tgctacacta
     4141 gaagaagcgc aaaaagctgt tgaagcaggc gcaagtgtat ttgtccatgc tttcaatggt
     4201 atgcgtggat tgaatcatcg cgaaccaggt atggtgggtg cgttattatc tttagaacat
     4261 gtcttttctg aattgatttg tgatggccat catgtacatc caaatgctgc gggaatccta
     4321 atggaaaaag caggacatga tcatgtcgca ttaattacag actgtatgat ggccggtggg
     4381 atgcctgatg gaaattacaa tttaggcgaa tttccagtag tagtaaaaga aggaacagca
     4441 cgtctagaat ctggcaactt agctggaagt atattgaaat tgaaagaagc aattaaaaat
     4501 gttgtagact ggggattagc aacaccagaa caagcagtca tgatggctag tctgattcca
     4561 gcaatcagct gtaaaatcga tgatacatgt ggaaaaatcg ctcaaggacg cgatgcagac
     4621 tttatcgtat tgaaccctga tatgacattg gaagcaactt atctagatgg cgtggaacgt
     4681 ttccacgcat aatataattt aagttaacct taaaactaaa cttcttaagt ttggttttaa
     4741 ggtttttttg tgttctagta aaaatcactt tacataagtt tcaaaagttt ctttaatttc
     4801 ctattgttca ataagtaaca atgtaaagat aaaattaaaa atgttaatac gtattagcaa
     4861 aataaaagct ttttttattg tgaataaatg aacataatta ggaggagaaa gatgaactta
     4921 tttgacattt ttcgatcgac tttgacagat atgtcgattg tcagtgcaat cacttcaaca
     4981 gtttttatta tattattagg gtttgtttgt agaaaaaagg ggattttttc tgaatctgtt
     5041 ggaaaaattc tatcgaaagt cgtcttaaca gtggctttgc cagcactggc atttcgagct
     5101 ttcatgcaag atatcaatac agaattactg atgcaaggaa tcaatgtctt gatttgggga
     5161 ttagttattt atattattct tattctagtt tcaaaacctt ttttcttaag ctataaaggg
     5221 gataagcaaa caacgttgcg tgtcctgacg atctttggtt ctacgacctt tttcggaacg
     5281 cctatcgtca gtgcgattta tggtcctgtc ggcgtgatgt attcctcaat tttcaatatt
     5341 ggctaccgaa tctttttgta ttcttatgga tacataaaaa tgagtggctt gaagatggaa
     5401 gctaaaaata tcaaagcgat gttcgtcaat ccaatcatta tcgctacttt tgccggcctt
     5461 ttcatttggc tgttccaagg gtatctgcca caagtgtcag ttacaggtgc agaaggagag
     5521 ataagccaag tagcgttttt gcgaattgac cagactgcac cgtggctatt tcagccgatg
     5581 gattatttag ctggtttaag ttcaccattg gcgtggctag caatcggcgc aacattaggt
     5641 gaagtaagct tcaaaaaagc tgcatctgaa aaaacttcat ggtattacag tctagtcaaa
     5701 gtggtatttg tacctgcatt gaatatcgtg attctagcag ttttgacggc aacgaatatt
     5761 ctaccagtca attttgaagc actggcaaca atcgttatca tgatggctac accagctgca
     5821 acagtggcag cagcctatgc tatcagtttt gatagggagg cacttttagc ctccaatgca
     5881 tccttactgt caactatttt tgctgtggta ctgacacctg tatggatcgt tgtattacaa
     5941 atgattcatg gcataggttt attttcataa gaaatggagg aagaaagtat gagaatcgca
     6001 tgttacggtg ttcgtccgaa cgagatagat ttttttgaaa aattgaatat ttaccattat
     6061 gatctcagtc tttatgaaga actgttgaca catgacaata tcgtgtcagc aaaggaccat
     6121 gatgctgtat tgttgagggg aaattgtata gcagatgaga ccaatcttgc aaaaatgcag
     6181 gaatatggga ttcgttatgt ttttacacgg actgtgggag tcaatcatat cgatctacaa
     6241 gcggcagctg attttggtat gacagtagcc cgtgtaccat cgtattcgcc aaatgcgata
     6301 gcagagttat ctttgacttt tgccatgatg cttttgagaa atactgcgta tacgacgatc
     6361 cgaacatctt tcaaagattt tcgagtggat gagcaaatgt ttagtcgtga aatcagaaat
     6421 tgcacagtag ggatcatagg aacaggcagg atagggttga cggaagctgt tttgtttaag
     6481 ggactaggag ccaaagtgat cggctacgat attcatcaga cagaagctgc taaaaaagta
     6541 ttgacgttcc actcgcttga agagttatta gccaaaagcg atattgtcag tattcatgta
     6601 cctcatatcc caggtgaaaa cgatcaaatg atcaatgaag gatttttagc tcaaatgaaa
     6661 aaaggtagca tcttgattaa tacagcccgt ggagagcttc aagataatga agctatttta
     6721 agagctttga aaagcaatca tttagctggt tttgcaaccg atgtttttgc aaatgaaaca
     6781 gaggtttttt tccgatcctt caagccatgg gagacgattc cagaccctgc tatccagcaa
     6841 ttagtagaaa tgtaccctag agttcttgta accccgcatg taggttcaaa tacagatgag
     6901 gcattatcaa atatgatttc gacaagtttc gagaactttc gtgaaataat agaaactgga
     6961 aaaacgaaaa atgaggtatc tcttcctaaa gcccgtcaat taagataaga aaacatggat
     7021 attcgtcaat aaaatgttta aaaatagtcc aagtaaacag ttgttacttc tgtatacttg
     7081 gactattttg tatgggataa ttcgatgact caacagtaat ctgtgagctt tttccgccca
     7141 ttctttgtca attcataaca ggttagcggc tggccgattt ccaaatattt gattctttcg
     7201 ttcaatatat cttgttctac taaaaaattc aaatatttct tgacggatat acgtgacagt
     7261 gagctttttt gagccagttc ttttgttgaa aaaatagtat cttcttttag aataaatgca
     7321 cttatttttt ttaaagtcag ttttgacaga cctttaggca aatcgatgaa tgatggtatt
     7381 tcgtgaatgg gagtggtgtg gttgaatact tgatcgatca atttctgatc ggctttttca
     7441 gtcgtttgga ttgtctgaga gaaagcaaga tacttttcga tcgccagctg aaaacgttgc
     7501 aaagtgaacg gtttgatcaa ataatctaag gcaccgtagc ggatcacatt ctttatggtt
     7561 tcgatatcat ctgcagcagt gatcaaaatc actgggatat catttccttg ctgtctaagc
     7621 caagataaaa aatggatacc actttcgcca ggcatgtata catcaagcag cagcagatct
     7681 gcgttttttt gtctgaaata tttttttcct tcttctacat ttctgaaact ttcgattacg
     7741 ttcatattag ggatgctttc cacaaaagta cgattcaatt ctgcaaccat tggatcatct
     7801 tcgattatca ggacatttgt catcattttg atcttccttt tttggtaatt ctagatagac
     7861 gattgtaccg ccatttgttt gtttggaaaa ttctaaaaat ccaccgtggc gtttgacgat
     7921 tgaatggatg agattcaatc caaacccacg attgattccc ttcgtcgaaa aacctagcgt
     7981 aaatattttt tctgagattt cttctgatat cccgacaccg ccatcgcaga cggtcacgat
     8041 gaatacttca gcttctgtgt catagtttaa atgaatccgg acctgtccgt ttctttcatg
     8101 tttttgtagc acggcttctt ttgcattatc aagtaagttc cctaacactt gcaaaagtga
     8161 atggatggag tcatccatct ccattttagg gataaaagaa tcgctttcta acagcatttc
     8221 aacagattgt tctttggctt cgtttatttt gcctaataaa aaaccggcaa ttgcaggtac
     8281 tttgatcaga cttgagactt ggccaatctc ttttttataa ttttgattta gctgctggat
     8341 aaaatgggtg acttcttcat acttcttcaa atcaatcaaa ccagaaatga catgaagttt
     8401 attcatgaat tcatgggttt gagctctcat ggagtttata tattgtttcg tccccccaag
     8461 ctcctccatt aatttcatta cttctgatcg atttcgtaaa gttgcgactg ctcctaaaaa
     8521 acggtcattg ctaaaaagag gtgtaacgct agaaatagtt tctatcccat ttaaatacat
     8581 ggattgagtc cgaacggtcg tttgatgctc aatcgtctct ttaaacaaaa cgtcatatag
     8641 attatcactg atttttttcc tacttcgaaa tccaatagct gctctgtttg gtagaaaatg
     8701 ttttttgcag gtgtattgat caagaggata gttttatcag aagagatggc taagattcct
     8761 tcatctactt cattttcgat aatcgttttt tcacttaact gttctgctat ttcttgtggt
     8821 tcaagtccaa gcagtacttt tttcattttt tgggcaagaa aaacggcgcc tatgatgcct
     8881 acgctaagtc caataatcaa tgaaaaaaca atcatttttt gtccatcttt gacttgattg
     8941 tcaatcgtgt tgtttgttaa gccaacacaa actgcaccaa taatctcttg atcataataa
     9001 atagggacga aatagcggaa gccgaaccct aacattcctt ctttttcaga gaaaaattct
     9061 tgtcctccaa tcactttttt cacatcttca tgacttgaga aaggaagacc gatgtaactt
     9121 gggtctgcgt gagaatatcg aatgccttca gggtccatga caacgataaa atctaaattc
     9181 gatgcaatca tgatttcatt tgttattcct tgaatcgcat gtgtcgggtc accagtctgc
     9241 agcttttttt ggatgagttt atcattggca acgattttag caatgctaga gattttatct
     9301 gacacactag atcggttcat cgaaagaata aaattcctga tcaatatggc agaaacaatc
     9361 aaagaaacga atgtggagaa acaaacgaca aggatgatcg tgttctggag ctttaatggt
     9421 tttttcttaa aaaaaggaaa cataggctac ctcgaaagaa tttattgtga aaattttaac
     9481 atgaaaacaa aaatttaaca aattgtcaac gataaacaca accttcctat aaaaaacacc
     9541 actgagtcgg taaaagaagc tatacagaat tttcaaacat tggtatgata gacgtagaaa
     9601 tggaaggagg cgtgtatatg atagggggaa tcgcacttga gacgatttat tggtatacat
     9661 tgctgatttg tgcaggattg gcagtcctat tgattatttt tggagatgta ttcgattttg
     9721 atggcccgct tgatccgatg cttattatcc cttggttaac tttcacaagc ttatttggtt
     9781 ttctaggtga gcgtttaaca gatcaatcat ctttattttt atttattgct agtggaggca
     9841 tatcgatagt gttggtattt ttgcttaatt tctatatatt gatgccgatg aaacatgccg
     9901 aatcaacgat gtcgtcttct gaaaaaactc tggaaggaca agtagctgtt gtgatcacac
     9961 cgatccctgt acgaggtatg ggagaaattc agttgaaaag tgttaccggt tccattagtc
    10021 gtccagcttg tttttacagc ccccaagaac agacagctga gcggggaacg aaagtattga
    10081 tcattgagat caaggaaaga atctgttatg tcatgcctta tgaaagcatg ataaaagctt
    10141 aaaggagcgt gtgttaaatg gaaataccag ttgtttttat cgcgatcgca atcgcagctt
    10201 tgattttact gatgttgctg atcgtctttg tagcgaagta ccagacagca aaaccggatg
    10261 aagcgttgat catcagtgga agttatttag gaagcaaaaa tgtgcacatt gatgaaagca
    10321 ataacaaaat caaaattgtg cgtggaggtg gcgcattcgt cttgccagtt tttcaacgtt
    10381 cgaatcggat cagtctatta tcgagtaaat tggacgtctc cacccctgaa gtgtataccg
    10441 aacaaggcgt accggtgatg tgtgatggta cttcgatcat caaaatcggt tcatctgtag
    10501 aagagattgc tacagcagca gaacagtttt taggcaagac aagagaagaa ctggaaaatg
    10561 aagcaagaga agtattagaa ggacacttgc gctcgattct tggttctatg actgtagaag
    10621 aaatctatca aaatcgcgat aagttcagtc aaagcgtaca agaagtcgct tctgtagacc
    10681 ttgcaaaaat ggggttgatc atcgtttctt tcactatcaa agaggtcaga gacaaaaacg
    10741 gttatcttga ctctttaggg aaaccaagaa tcgcccaagt caaaagagat gcagatatag
    10801 cagaagcgga agctctaaaa gaaacgcgta tcaaaaaagc tcaagcagaa aaagaatcac
    10861 aagcagctga attacagcga caaacagaga tcgcagaatc attaaaagaa aaagaactga
    10921 aattagcaac ctacaaacaa gaacaagatg ttgctaaagc aaaagcagac caagcctaca
    10981 atttagaaag tgcccgagca caacaacaag taatcgaaca ggaaatgcag attaaaatcg
    11041 tagaacgcca aaaacaaatc gaactggaag aaaaagaaat cacacgtcgt gaaaagcaat
    11101 atgattcgga agtgaagaaa aaagcagatg ccgatcggta cgctaaagaa caagaagcac
    11161 aagctcaaaa agtcaaagaa gtgactgaag ccgaagcaga acgattccgt gtggaagcac
    11221 tggcagaagc ggaagccaat aagacacgtt tagctggaca agcagaagcc gaagcagcac
    11281 tagcaaaagg aaaagcagaa gccgaagcaa aacaaaagat cgccaatgcc ttcaaggaat
    11341 atggcgaagc agctgtcttg agtatggtta tcgatatgct accgcaattg atgcgcgaag
    11401 cagcacagcc gctaggaaat atcgaaaaaa tctctgttgt cgatacagga agcagttcag
    11461 gagaaaccag tggagcgaac cgagtgacaa attatgccac aaatttgctt tcttctactc
    11521 aagaaacact aaaagcgaca acaggacttg acttgaagga aatcgtagag aatttgtcga
    11581 cacgtgggac tactagccaa gtaaatatgt atgaagcgcc aaaagaggca gaacaaaact
    11641 cgatgaagga agaataaagt agtacacttc accgttcaat caaaaatgga ggaagacttt
    11701 ttaaagttct tctctccatt tttttatttt attgcaaaag aaaattagtc ctcgctattt
    11761 ttcttttgtt tttggatctt agcttctatc tcttgtaaat ttgttcttag caatcgttct
    11821 ttcttttgga gttcgaccaa atcttctgat agttcttcct tcttttctgt ttcaggagga
    11881 cgaagaaaga aattgatacc gattgcaatg aatgtcccta taaaagtatc tagtacacgt
    11941 tggatagcat acaaagcgga ttcgccttga gggatgctca acgcaatcag caacaacgta
    12001 gctattgcgg aaatgatacc ggaattattg ttaataccat ctgacacgac gatcacaata
    12061 ataactagaa taggaagcaa gatcaattct accaaaaaat cattgtgaaa atagcttttg
    12121 acaaaaaaat aaatgatagc taatgtacca cctaatgtat tgccgatgat cctagacttt
    12181 ccaaaagaaa cggttgtagt caagtcctga cgcaaagaaa aaacagcaga taatgcagca
    12241 atcatcggga ttcctcgttg agtcaaatga aaaaacaaga tacataaaaa aacagctaag
    12301 gcacttttga tcgtacgcat acctagtcga aaacggccaa attgcataga gattctcctt
    12361 tttggtcttt ttactatcat acctaaactt ttgtctgata gctagttttt cattattcaa
    12421 gtataattaa aataaaaaag ggggaagtaa atgcgtatag gaatgcgaac agtcaaaaca
    12481 gtgatcagtg tcttcttttc gatggtcaca gcgggtagct tatcgctgtt gtattggcca
    12541 gctgcaggga tcattgcatt attaagtgta ggaaatacca agcgctctac attgcgtacg
    12601 ggcatgaatc gaatcgcagc ctttatatta gctacggcaa cagcactgtt cagttttttt
    12661 gctgttgggt atactatttt tggcttcagt ctttttttat tcctgttcat cccgcttgcc
    12721 tctcgcttca aattaaccga agggatagta gtaaattcgg ttttggtcac tcattattta
    12781 gtggaggaaa acatgtcttg gcagctgatc ggcaatgaag cgggattaat ggcgattgga
    12841 ttagtatttg ctttgcttgc aaatgtttac atgccagatc gtacgaagca gctgaaggaa
    12901 aaccaaatac agatcgaaaa agaatttcga aatattttga gacaaatggc agaatttctt
    12961 ttatcagaaa ataaagaaga tgtgcagatc aaatgcgaac atttactgac attcattcgc
    13021 gaaagccaag aagatgccag agaacatcaa gaaaactact ggctcagaca gccgctatac
    13081 tacgaaactt acttctctat gagaagagca caggccaatg tgattaaaga catgctggaa
    13141 aatctagaga gaatccagca accagcttat tatggaaaac atatctatgg tttattgatt
    13201 tatactgcag aaacattttc agagtcaaat gatggcagac aaattttaac acgtattgaa
    13261 gaagtttatg tattgtatcg gcaaatgcct ctgccgacta gtcgccctga attcgaggat
    13321 cgagcagaat tgttccagtt tttgcagtct tttaaaagtt ttattgaaat caaggcagaa
    13381 ttctcccaac aaaaaatcca aaaatagagg ttgtgaattc agctaatatt caggtcaaaa
    13441 agctgatttc acaacctctt ctttaaacgg tgttctcctg tcaactcctc ggtcacaagt
    13501 cacaatattc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg actcggagct
    13561 gaacggtagg ctcacaacct ctttcttaaa cggtgttcaa aagctttcct ctgcggtaat
    13621 aagtccagcc aaacgaaaaa taggaggagc tatttttgtt tggctgttct tattacttga
    13681 tgcagaacag ctttttcaca accttttaaa attacatatt tttccataga acttgcattt
    13741 gatccaagat ttcgatgaca tcttttgtca agaagagaga gcgattgggc aagtcacctt
    13801 caatcatgtg taccatattc tttatttcgt aattcattgc ttcagatgta ttcccgcttt
    13861 cgatgaattc ctttgtacca tctgtaaata agatctccgc acgatcagca cgcgggtaat
    13921 cagcaatcgt cacataaccg ttctcaaagg caacgatacc gacttttggc atttttgcct
    13981 gaaaagtcaa gctgacagca gcaagttcat tttctttgtt acgcaagata gtgacggatt
    14041 gttcgtccac gcctgtttca aaaggaacca ttgtagaggc aactacttca ggttgagagc
    14101 tcaaaaagaa acgagcgaaa gatacagcat acgtaccgat atccaacaga gctcctccag
    14161 ctaattctgg attgaagaac cggtttttag ggtctggttc tttgtaactg ccgaaaggtg
    14221 cttggatcat ttttaaagcc cccagttttc ctgtatccat tattgagcgt aactgctgat
    14281 aaagtggcat gttgaagatt gtcatcgctt ctgctaagat caaattcttt tcttctgcta
    14341 agcgcatagc gtcagccagt tcttttttat tcatcgtgat ggctttttca catagaacgt
    14401 gtttgcctgc ttccaatgct gccaagatat ggtcgatatg ctgtctattt ggtacagcaa
    14461 tgtacacgat gtcgatttca gcatcgtcta acatttcttg atagcttccg taagcttttg
    14521 gaatattgta acggtgagcg aagttttccg ctttttctga agttctggaa gcaactgctg
    14581 ttagttcgct cgtctcttga ttgaagcttt cggcaaactc atgtgcgatg gtaccaagac
    14641 caacgattcc ccattgatat tttttcatct gaaaacgctc cttcatagtt ggattgagtt
    14701 tcttgtctgt ctattattat aacaaaaatc acgaagaata aagaggagag gacttgaaac
    14761 attaaaaaaa atgaaagcag cgatttaaga ggtgcttttt gatcaaaact taagtagaat
    14821 agttaaaagg aggggataaa cgtgcatcaa gtatatattg atcttattat gagtgtatta
    14881 ttagaacagt ttgaaactga aaaaaatttt ttagatgaat acttgctgat cagtccagaa
    14941 caatggcaaa aatggaaaga tggcgagaaa aatctgccat ctgaagcgat gcagaaagta
    15001 aaaaatcttt tcactgatta tgaatggatg ctaacgcaaa agattttgcg tcaaacgatt
    15061 atttttcccg aaaaacgaaa cgttgccgtt gcagagtaca agcaaatcaa aacacgcatc
    15121 gcgcaaaaat ggcttagcag caaagcagca aaagtcgagc tgatcccttt gaaaaataat
    15181 cagcaggctg gtagatacct tgacttgaaa gtaagtattc aatatgatga atggggctat
    15241 gatgatattt tgaattttcg tctgccagca gatgttcaaa aacagatcga aggagaaaaa
    15301 atcgaacttc tcgattgggt gaatgaaaat ctggaagaaa cttacgttca tgaggcgaat
    15361 ggggaacgtg aagaacaacg aaaggatttt tcaagtgaat aaatttttag gatttggagc
    15421 agttgtgatt atgatcgcag caatgggcta tgttattttt agcgatgatg attcgcatgt
    15481 tcaagccggc aatcaagcag tcgaacgaac aagcaccaaa acaactgcta gtaaagaaaa
    15541 gaacagtcaa acaaccagca gcaaggtaaa ggacaaagaa acaaaagatc gtacgtccaa
    15601 acaattgcca gatgttaaaa cagacgattg gaagctagtt ttggttggtc cagatcacaa
    15661 attagaaaaa gaaattgatg agtctactca attggcatca ttaagtaacg gatacttgat
    15721 cgacaaacgc attgctccag aatatgaagc atttgccaag gctgctgaag cagctggatt
    15781 tccattagtg atggtctcag cttaccggtc tgtatcatcc caacaacaag ttttcgaaca
    15841 aaatgtccaa gatgtgatga gtcgtcaagg tgtatcagaa gaggaggcga caaagattac
    15901 taaagaaacg attacggagc caggatacag cgaacaccac acaggtctgg cagtcgatgt
    15961 cgtcgatcaa aactggtaca acagttatcc ttctactgta ttggatgcaa gttacggaga
    16021 tcagcctgga gctaaatgga tcgcagataa tgctgcgcag tatggattta tcgttcgtta
    16081 tccaaaagga cacgaagata taactaagat cacatatgaa ccttggcact tacgttatgt
    16141 cggtaaagaa aacgctgagt atatcaccaa acaccaattg acgctagaag aatatctgaa
    16201 tgaattaaat gaaaagtagg aattgatcat ggcgaaaaaa aagtggaaaa agcaaaagcg
    16261 caagaatatc cgttggccag cagtggttgc cggtgtagca gtgatactct tagcctttgt
    16321 tttttccttg aagaatctgt cttctccttt tactgataat cagcaagaaa atgaagcgca
    16381 atcccatcag caatttattg aacggcttgt tccgcatgcc caagaactac aagatggtta
    16441 tggagtgctt cctagtatca ttttaggaca agctatatta gaatcaaatt ggggaaaaag
    16501 taccttggct agcaaataca ataatctttt cgggatcaag gcttatgggg atcagaagaa
    16561 agtaagcctg gaaacaaagg aatttgttaa cgaagaatgg ataactatcc aaggagactt
    16621 caaagtgtat gattcatggg aacagtccat ggatgaccat acacaactgt tcgtccaagg
    16681 agtagattgg aatcctgcgc tgtatgaaaa agtgattacg gccaccaact atcaggaagc
    16741 cgcacaagca ttgcaagatg caggttatgc gacagacccc ggttatgcgc aaaaaatcat
    16801 ccaggtcatc gagacgtatc aattgaatca gtatgaccgg agatagcaaa ggagaagtgg
    16861 ctgatggcag aacaatttac gccagaaatc atgacggaat tgagaaaaga tatcgtaaga
    16921 gtaccggaaa tcattttaga agcaagcggg atcaagatat ttggaaaatt gatccgttcg
    16981 atcatcttta ctacggatat tgcgatcata cgtaatacaa atgcgaatgc agtaattgct
    17041 gtgtatcctt ttacacctca tccagcgatt actaaaagca ttattgaggc agctgacatt
    17101 ccagttttct ccggtgtagg tggtggtttg acacaaggtg ctcgttcttc ctacatgagc
    17161 atgttcgcag aagcgcaagg ttcgttaggg gtcgttttga acggtcctac gcctttagag
    17221 acagtaaaag aagtttgtcg agtagttgat attccagtta tcagtacggt aacgtcgaaa
    17281 tatacagaaa tagatgaaaa gttagcagct ggtatttcga ttatcaatat cagtgcaggt
    17341 aaagaaactg cagaagcagt acgccatttt cgtaaaaaat atcctgaact tcctatcatt
    17401 gcgacaggtg gtccaacgga agaaagtatt gctgagacca ttcaagcagg agcaaatgcc
    17461 atcacatata cgccaccaag tagcggagag ctttttagtc gtaaaatgga aaaatatcgg
    17521 gaaactgaga aaaagaatca tgaataacag gtgagaatca aaagacgagg aagagcagat
    17581 agtggctctt cctcgtcttt tgtactccag tactatttgt tcagaattca aaaaaaagag
    17641 aaattttttt gtttttttac ctgtaatgta cataagaaga gttaaaacct tttgttatta
    17701 ttagcattct aacaaaaaaa tgtcttgatt tattgaagcg ctttctatag aatgaaaaac
    17761 gaataaaaac taaaaaaggc acccgacaat ttagtagcag ctaaattgtc ggggcttgaa
    17821 aagtcagcgg gaactgacca atcaagttgc catagtcaat gcttgtgcat cgacgtgtat
    17881 attgtaccta atttcataca gaattactag ttatttttct gttttttagg gaagagcgca
    17941 cacgaagaag catgcttatt tagctatgtc aaggatattg ggcagttgtc tgatatcctt
    18001 ttttctttgc gtaattatcc tcccatcatt gaagcaagaa gtgtttttag cgatgaaaga
    18061 agaatcagca ctatctaatt tttcttgaaa gtattgagtt gttcatccta ggcagaacga
    18121 taaggcaagt cttggttttt attccccttg aaaaagatcg tttaataaat atgttctgta
    18181 taaaaataca gtatattgaa aaataacaat ggaagtagag gtgcagagaa tgaagaaaag
    18241 aatgagaatc atcttagctt taagtgttat gttattcttc ttgacggctt gtgggcaaga
    18301 agatgacaaa aaaatcacgc tgaacatggc ttggtggggt tcgcaagtcc gacacgatgc
    18361 aacagtaaaa gtaatcgagc tttatgaaga aaaaaatccc catgtgacga tcgattatga
    18421 attttatgat tatgaaggat attacacaaa attaggtaca ctagcagcat cagatgatat
    18481 ctgggacatt ttccaactga acaatcaatt tcctcaatac atcaatcaga ttgaaccatt
    18541 agatgaatat gtggagcaag ggataattga tatttctaat gcagatgaat cctatttagc
    18601 aacgacgact tacgacgggc aattagttgg actgtctaac ggggtgaatt cgtttgcgat
    18661 tgcttacaat aaaaatatat ttgaacaatt aaatatccct gaaccagctt caaattggac
    18721 atgggatgaa tttgaacaaa tcagctatca aatcactgaa gaattaggaa tctatgctat
    18781 ttcaagattc gaagatttta tagctggctg tattattcaa ataccacaag ttgagaaagg
    18841 attgaatttt tataatcggg aagatctatc taacagcttg ggatttgaaa atccagacta
    18901 tctaacagat tttttccagc tgcgtaaaaa tctggtagac aaaggggccc atccagaacc
    18961 aggtggtgga gctgcaagta cgacggatct gtcttttcag gcggttaggg atagagaagc
    19021 ggcaatggtg tttctatcaa gtaaccagtt gacagaaata ttgtcagggg ctcctgaaga
    19081 tatggagatt cagttgatta atccacctcg cagaaaagca gatggagaat ctggtatccc
    19141 tttacgttct tcgcaaatgc tgagtatggc aaaaagctca gcccataaag aagaagctgc
    19201 taagtttatt gatttctttc aaaacagtga agaagccaat gaaattttaa aaggagaacg
    19261 tggtgtacca atcatgtcga atattcgaga acagctcgct cgtgcagatg atgcaacttt
    19321 gcaagcgaca tttgattatt tagaaatgat ttccgatatg gacaacggcg aagtgaatgt
    19381 ctttgatagt gctttgaatc cggaaattca agaccaatac aatcttttag ttgaaaaagt
    19441 catttatgaa gaagtaacag cagaaggaag gcatgaaaaa agaaaaacta aaacggttct
    19501 tggatcaaga caatgtggtc ggttacgtat ttgcttctcc attcatccta ggatttctat
    19561 tgataacagt gataccgatg atcttgtcac tttattattc gttcaccgat tatcgtttgg
    19621 gaaatccagt ctcatggatc ggcttggata attatataaa gatgctgagt gatgcccgtt
    19681 ttttgaactc catccgagtc acttttctct atgtatttgc atctgtacct gtgaaactga
    19741 tctttggtct attgattgct tatcttttaa cgaggaatat caaaggaacg actttctttc
    19801 gttctttgta ttatgtgcct agtttgatcg gtggcagtat ttctatcgct ctagtttggc
    19861 gggagttgtt ttctaaaaac gggttagtga atattttcct ggaacgatta ggcgcacctg
    19921 cgctttcctg gtttagcgat caaaaactgg tcatgatccc tcttgttttg atgtctgctt
    19981 ggcaattcgg ttcggcaatg atcatctttg ctgcaggtat caaagagatc ccaggctcgt
    20041 attatgaagc ggcagagatc gatggagcga acaaatggca gacgttttac aagatcacgt
    20101 tacctcttct ttcgccgatc attttgtata acttggttat gcagacaatc gcggcgttta
    20161 tgacttttac gcaggcattt atcattaccc aaggtggtcc gaatgatgcc acgaactttt
    20221 atgctcttta tgtgtataac caagcttttt cttttaattc tatggggtat gctagtgcta
    20281 tttcgtggat catgctgttg attatgggag ctgtcacctt tctgattttc aaatcatcaa
    20341 aaaaatgggt cgttgtgtga atgagaggag gaagaaatga ataatacagt aacgaaatgg
    20401 tttcaatacg gttttatttt gctattaggt ctagtcatga tttatccggt cttatggatg
    20461 atcgcaggtt cttttaaaac ggatcaagag attatgagtg gttcgctgaa tttgattcca
    20521 gagacttttc aatgggaaaa ttacgcaaaa ggatgggcgg ggtttgctgg aatttcattt
    20581 tttactttct ttaggaattc gtttgtcatc tctgcagttt cgacgatagg gacagttctg
    20641 agctcgactt gtattgctta cgcattatcg agaatcgatt tcaaaggaaa aagattctgg
    20701 ttcgtcgtca tgttagtaac gatgatgatt cctgcacaag tgattttgat tccgcagttt
    20761 attatttaca atcggctgaa tttggttggg acgtatgtgc cgctgatttt accacatttt
    20821 tttggacaag catttttcat ttaccagatc atgcaattca tggtcagcat ccccaaagag
    20881 ttagatgaat cagcgattat tgacggatgc agcaagtata gtgtctttac caaaattatt
    20941 tttcctttgc tgaaaccttc gatcatcacg acgatcatca tccagtttta ttggaaatgg
    21001 gacgacttca tgggaccatt gatttattta aacaagccga gatcttacac ggtatcgatt
    21061 gccatcaaat tatttgcaga cgctggatct agtacgactt acgcgagtat gtttgcgatg
    21121 tctacgttgt cgctgctacc ggtttttctg attttcttat tcttcaataa atatcttgtc
    21181 caggggatca gtacaagcgg tctgaagggc tgaatatgaa aaagccaata gaagagatgt
    21241 ttctttacaa gctcaactac tatgtctgtc ggattttttt aaccagtctt atcagtaatg
    21301 ctcttttcgc tttagttcta ggatgtttgt tcactttagg attagtgctg tctgctcgtg
    21361 ttgtctatct gatttatttt ttgatgatgt atccttctta ttatctatct gtccattact
    21421 tggcagaaaa aaaggtatgt tggaggaaag ggaaaatcaa acggatgtgt ttcatcatta
    21481 cgttgtttca gctgtttgtc attatttttg cagttgattg gtatttatta gtgggaaaga
    21541 ttggagcttg gtatctgatt ccttttctat tgtgtctgtt gctttttgta tcccatagtt
    21601 tttttagtac gctaattgat atctgcaaag aagataaagc agatagtctt gtttatttga
    21661 tcaaaaggaa tatctggctg ttgtcaaaaa acgtcaaggg gtttctgtgg tatttatgcc
    21721 ttttgtatgg actgttctgg tgtatacagc ctgatttttt actcactgca ttactgtatc
    21781 cagggctgcc atttgtcaca gtattttgct taaaagaaaa ggagaatgac tttaaatgaa
    21841 gaaaaaatat gtacaagtcg gacttggtgg gagagcgcgt tttttttatg aggcaattgc
    21901 ctctacgtat catcaaactt gtgaactagt cggtttttgt gatgtatctc aaacaaggat
    21961 ggattatgca aatcggatac ttgtgaacaa gtatggacag aaaaaagtaa agacatgtat
    22021 gccatcacaa tttgagcaac tactggatga cgtacggccg gctgaagtca ttattacgtc
    22081 agtagatcga acgcatcatg attatatcat tcgtgcaatg aaaaaaggat atgatgttat
    22141 ttgcgaaaaa cctctgacta tcgatgagga aaaagctcaa gaaataataa cggtccaaaa
    22201 acagacggga agaagcctgc gagtcacttt taattatcgt tatgcaccgt atcacacgaa
    22261 gataaaggaa ctgatagaaa gtgatgtgat tggtgatgtg ttttctatcc acttcgaatg
    22321 gctgttaaat acgcaacacg gggcagatta tttcagaaga tggcatcgaa ataagcacaa
    22381 tagcggcggg cttcttgttc ataaatctac ccatcatttt gatctagtga atttttggtt
    22441 gaacagccgt ccccaaaagg tatttgcatt cggtggatta aacttttatg ggatgcataa
    22501 tgcggagaaa cgcggagtag aaatatttta cagtcgatgc tatggaagcg aactagctaa
    22561 agctgatcca tttgctatag acctgtcagc caatgaggaa ttgcgagaaa tgtatttaga
    22621 agcagaaaaa gacagtggat atattcgtga ccgcagtgta ttttccgatg atatctctat
    22681 tgaagataca ctaggcgtaa cggtcaaata tgaaactggt gcgattttga cgtattcgct
    22741 aaacgcgtat atgccgtggg aaggattcaa tgtttctttc aatggcagca aaggacggat
    22801 cgaaatgaaa gtgatcgaaa agtcttacat caatgcaggt ggagataaaa aggatgaagg
    22861 agcagccgtt cttcagtcca tccatgtcta tccgatgttc ggtgagccat atgaggtaat
    22921 cgtcgaacaa gaagcgggcg gacatggcgg aggagatccg cgtttattag atgatctatt
    22981 tggtgaagaa aaagaagaag atccgtggaa tcgggcagca actcacgtag atggtatttt
    23041 gtcgattttg acaggaatcg cagcaaatca ttctattgct tcaggaaaag cagtagtgat
    23101 tgacgagctt gtcagcttaa aagaataaga atagatttac ggaaattact tcccgtagtt
    23161 tataaaatct tgccttattg ccgataggct gtcttttgaa cactatttat tcggaaaaag
    23221 gggctatgac atgctttttg tcatgccttc tttttatgtg ggctgctttt ttagatagga
    23281 gaaaataaaa ataactattt aaacccgaac aaaaaggaat aatgcattaa aaatcattag
    23341 attttacaaa aaaataagta aaccacgttg tcagtttcag aaagtagtgt tagactgttt
    23401 tcaatatttg aaacaacatt ttttattcaa atatggcaaa ctgccttcac gtgcaaaaca
    23461 gttgcaagaa aatgaattta cactattatt agttttacat acctacttat atatcgtcat
    23521 aatatggatg acagtttcta gccagtaccg taaatgctgg actataagta aaagattggc
    23581 tatttcaccc ttcttttctt atgataaagt ggagggtgtt tttctttaac agaaacatag
    23641 taggtttttg cacaaaagcg gttatttcgg tattaataaa tgataaagga gcaaataaag
    23701 tgaaagaatt agttgaacgt attcaaaaag acggacgtat attaggcgag ggcgtattaa
    23761 aagtcgacag cttcgtcact caccaagtag accctgtatt gatggaacaa atgggaaaac
    23821 ggtttgcaga agtatttaaa gaacaaggaa tcacgaaagt tgtgaccatt gaagcttctg
    23881 ggatcgcacc agctttattt gctgcaagag aattagatgt tccaatgatc ttcgctagaa
    23941 aagcaaaaag tttgacgatg gatgaagaat tattgacagc ttctgtttat tcttttacga
    24001 aacaagtgac aagtacgatt tctatttctc gtaaattttt atcttcagaa gacaaagtat
    24061 tgattatcga cgatttcttg gcaaacggac aagcggcaaa aggattgatc gaattatgcc
    24121 agcaagctgg agcgcaagta gaaggaatcg ggatcgtcat cgaaaagtct ttccaagatg
    24181 gacgtcagtt acttgaagaa atgggattga gagtcgtttc tcttgcgcgt attgcttcct
    24241 tgacgaatgg acaagttgag tttcttgagg aggacgcata atcgtggaaa aagaaacaca
    24301 aaatggaaaa gctgcggttt tagggctgca gcatctgcta gcgatgtatg caggagcagt
    24361 agcggtgcct cttttgatcg gtacaggact aggctttaat caagaacaga tgacttattt
    24421 gatttcaatt gatatcttta tgtgtggatt agcgacatta ttgcaattga cagttaaccg
    24481 atttttcgga attggcttgc ctgtcgtttt aggatgtgcg atccaagcag tagctccact
    24541 gatcttgatc ggaaccaatg aaggagtagg agctatctac ggatctatta tcgcttctgg
    24601 gatctttgtt gtgttagttt ctggtgtctt ttctaaaatt aaaaaactat tccctcctat
    24661 agtaacagga acggttatca ccgttattgg tttgactttg attcctgtag caatcgagaa
    24721 aatgggtgga ggtaatgcaa cagcagctga ttttggtgac aaaacaaatc tgctacttgc
    24781 atttgtgacg attctcctaa ttgtaggcgt gcaaatgctg gcaaaagggt tcattcgttc
    24841 aatcgcggta ttgattggtt tagtcggagg aagcatctta gctgctttca tgggaatgat
    24901 tgatctgagc gcgattggag atgcgccgtt gttccatgtt ccacaaccct tttattttgg
    24961 taaaccaact ttcgacgtat ggtcgatttt actaatgatc atcatttcca tggtcagcat
    25021 ggtagaatca acaggtgtat attttgcact tggtgacatt actgggaaaa atgttggaga
    25081 agaagaattg aaaaagggat atcgtgcaga aggacttgcc gttatcttag gcggtatttt
    25141 caatacattc ccttatactg gcttttctca aaatgtcgga ttggttcaac tttcgggaat
    25201 caaaacaaga aaacccatct atttctctgc attcttcttg attgttttag gattattccc
    25261 taaaatcggt gcgttagcac aaatcatccc tgagccggta cttggtggag gaatgttagt
    25321 catgttcggg atggtggcag tccaaggaat ccgtatgctg ttggaagttg atttcagcaa
    25381 tgataaaaat ctattgattg ttgctgtttc tgttggattt gggctgggtt tcaatgttac
    25441 gccgacatta ttcagtcaaa tgccgcaaac attgcaaatg ttcactggaa atggcatcgt
    25501 aatgagtagc attacggcta tcatattgaa tttgatcttt aatggattga aaaaagatga
    25561 agaaatattg gaagaagcct tcaagaagta atagagcaaa agaaaggttg tgaaatcagc
    25621 gttttgacct gattgttaga gaaacaaacg gaaaaatagt tcctcgtatt ttgccgtttg
    25681 tttgagctaa taccgagggt cagctgattg aacaccgttt atccataaaa aggaatgtga
    25741 cctaactttt gtcacattcc tttttttcat ctttaataaa cacgaacaat aaaatgtttt
    25801 tctttgttaa cattcgaaat ttctttgaca tcaaaaaaag tgattgttaa aatatgaatg
    25861 tagaaaaact gggaggaaaa acaatgacag caaaaatctc tgtgattatg gggagtactt
    25921 cagattggga aacaatgaaa gaagcatgtc aaatattgga tgaattcggt gtgtcttatg
    25981 aaaaaaaggt cgtttctgct catcgaaccc cagatttgat gtttgaatat gcacaaaacg
    26041 ctcgaaaaaa aggaatcaaa atcattattg caggagcagg gggagccgcg catctgccgg
    26101 gtatggtcgc atcaaaaaca actttgccgg ttatcggtgt accggtccaa tcacgaacat
    26161 taaatggttt ggattcgctt ctttcgattg tccaaatgcc tggaggagta cctgtagcta
    26221 ctacagcaat cggaaaagcg ggagcaatta atgctggact acttgcgata cagatgcttt
    26281 ccatgtatga tttagaattg gaggctaaat tggctgaaag acgttcaatg ttggctaaaa
    26341 ctgttataga aagtagtgat caacttggtt aaacctttgt tgcctggttc aacgatcggg
    26401 atcgtcggtg gcggtcaatt aggtcgtatg atggcgatta gtgcaaaaga aatgggcttt
    26461 aaagtaggtg tgctggatcc tgtgagcgat tgtccagccg cacaagttgc agattggcac
    26521 attgaaagtg tctatgatga cacctttgcg ctgaaagagc ttgccagaag aacagatgtc
    26581 attacctatg aattcgaaaa cgtcagtgta gaggcgttaa atgcgatttt acccatgtca
    26641 tttattcctc aagggacaga cttgcttgcg attacgcaag accgcctgtt agaaaaatct
    26701 tttttggaaa cgaataacat cgtgattgct ccttatgcaa cgatcgtcag tccgacggat
    26761 atccaagatg cgattgacgg gatcggctat ccttgtgttt tgaaaaccac tcgaggcggc
    26821 tatgatggca aggggcagta tgttttgaaa agtcgggcgg atctagctcc tgccatggac
    26881 cttttaagag aaggaacctg tgttttagaa gcatggatcc cgtttgaaaa agagatttcg
    26941 atcatggtgg caggaaatgg tcaaggagat ttcacgactt ttccggtcgt ggaaaacatc
    27001 caccacaaca atattcttca tgaaacgatt gctccagccg cgattgatca ggatgtgatc
    27061 gaggaggctg agcgtattgc ccgtgtgatt gcagaagcag tgagcctttc aggtgtttta
    27121 ggggtagaaa tgtttttaac aaaaacaggc gggctatatg tgaatgaatt agctcctcgt
    27181 cctcacaatt ctggacatta ttctattgaa gcttgttcta tgagtcagtt cgatgcccat
    27241 attcgaggta tttgtggttg gccattagga gaaaaagtac gtttgataag caatgcagta
    27301 atggtcaatg ttttgggaga acaactgctg gaaagctatc gcttgattga gaataagcca
    27361 gactggcaat tccatttcta tggaaaaaaa caagcaaaaa aaggtcgcaa gatgggacat
    27421 atcactatct tgacagaaga catccagcaa acgctggaag aaatcaaaca aacgaatatt
    27481 tgggattaga ggagaaaaaa catgttagaa cgttatacac gccctgaaat ggggaatatc
    27541 tggacggaag agaacaaata ccgtgcgtgg ctcgaagtcg agattttagc agatgaagct
    27601 tgggcagaac ttggtgaaat cccgcaagaa gatgtgaaaa aaattcgtga gcatgcatca
    27661 tttgatatcg atcggatctt ggaaatcgaa aaattaacac gccatgatgt cgttgcgttt
    27721 acacgagcgg tttctgaaac attgggagaa gaaagaaaat gggtccatta tggtctaaca
    27781 agtacggatg tcgtagatac tgcttatggc tatctcatca agcaagcaaa tgatatttta
    27841 cgggaagact tgaaacgttt tacggaaatc attggcgaaa aagcaaaaga acacaaatat
    27901 acagtgatga tgggtcggac acacggtgtc catgctgaac caactacttt tggattgaaa
    27961 cttgccacat ggtattcaga aatgaaacgg aacatcgaac gatttgaaca tgcggcaaaa
    28021 ggagtagaag caggaaaaat ctcaggagca gtgggaacgt tcgcaaatat cccgccattt
    28081 attgaagagt atgtatgtaa acaattgggg atccgtccac aagaaatctc tactcaagtt
    28141 ctccctcggg atctacatgc tgaatatttc tcagcgatgg cgcttattgc tacaagtatt
    28201 gaacgttttg caacagagat acgcggattg caaaaatccg aaacacgtga agtagaagaa
    28261 ttttttgcga aaggtcaaaa aggttcttct gctatgccgc acaaaaggaa tccgatcggt
    28321 tcagaaaata tggctggtct agccagagtg gtgcgcggac acatggtcac tgcctatgaa
    28381 aatgtcacat tatggcatga aagagatatt tctcattctt cagcagaacg aatcatcttg
    28441 cctgatacca cgattttatt gaattacatg ttaaatcgtt ttggcaatat cgttaaaaat
    28501 ctaacggtct ttcctgaaaa catgaagcgg aatatgtctg caacatttgg tttgatttat
    28561 agccagcgcg ttatgctgaa attgatcgat aagggaatga caagagaaga agcttatgat
    28621 cttgttcagc caaaaactgc gtacgcttgg gacaaccaag tgcagttccg cccgctgtta
    28681 gaagaagatc caaccatcac atcggtatta tctaaagaag aaatagacga tgcctttgat
    28741 tatcatcatc atttgaaaaa cgttgacgtg atattcaaac gagtaggttt ggaataaaga
    28801 agagtgagtt gcaaaattca attaaaatag aatagcaaaa ataaccaaaa aagaagcaga
    28861 tccgttagcg gttctgcttc ttttagttta ttatattttc ttatcaataa taggaacctg
    28921 attttaaaag cggctacctc tttaccgtgc ctctttacat acaaaaaata gaggaattaa
    28981 gcgtaaatgg cttggctatt ataactaaaa tgctggttat ggagatgttg cagcaagttt
    29041 tcgagaaaag cgtattccat ctcttgtcca ttgaataaga tctggatacg ataatgattt
    29101 ttgaaacgct tctgttcatg aatctgactg attgaaagtt cgcctttgtg ttctgtctga
    29161 taaagaaaag tccttttacg gcattcagca cagcgcttgg ttgctcggcg gatttttcgg
    29221 aatgtataaa aactagtatc tttattgcat aaataaacgt ctttgatcga cagcaaatca
    29281 ttatatagac aggaacgttc ttcagctgtt aattctgata aaaaaagctg accttgtttt
    29341 attgcatctt ccatcggcaa gtgcctcctc gaacccatta tccaaatttt tagttaattc
    29401 taatatacca tcattaagta taaatgtaaa cgcttgttga tggtataaga ttttcgtttc
    29461 tgataaaaca cgaacattaa aagaatctaa tgccttaaat gtacatattt ttgttgattt
    29521 ataaaaagat gattgctaaa atttaaagtg aaaaaacgaa caaaaaacga tgtatttgct
    29581 tttaatgtaa ataaaaagga gagacgtaca tggagaagaa agaacttttg tatgaaggaa
    29641 aagccaaaaa aatatatctt acttctgatg agaacttgat ttggatagaa tatttagatc
    29701 aagcaactgc gttgaatggc gcgaggaaag accagataag tggaaaagga gaattgaata
    29761 atcaaatcac aagtgcaatt ttccgttatc tgactcaaag aggaataaaa aatcatttta
    29821 ttgaacaatt gtctaaaacc gaacaattag caaaaaaagt acaaattatc cctctggaag
    29881 tggttatccg aaatattgca gcaggaagtt tctcaaaacg tctaggtgtt cccgaaggta
    29941 aagaattcat caaaccaatc attgaattct actataagga tgaccagttg gatgatccat
    30001 ttatcaatga agagcatatc tatttcttac aacttgcaaa accggaagaa atcagtgagc
    30061 tgaaagccca agcgttggct attaatgaaa ttttgaccga attatttgct aaggtcggtt
    30121 tggttttagt agatttcaag ctagaatttg gccggtcgaa tggacaaatt ttgcttgcgg
    30181 atgaagtaac accggatact tgccgattat gggatgaacg tacgaatgaa catatggaca
    30241 aagacgttta ccgcagagat ctaggtgatt tgatcccagt ctatcaagaa gtatataacc
    30301 gtctatcgga actaactgaa gaaaaggagc gagtatgatg tatttcgtaa aagtatatgt
    30361 aacctataaa gattcagtgt tagatcctca aggggaggca gtaaaaaaag cagtccatcg
    30421 tatgggatat gaaacaatcg aagatatccg aataggaaaa tactttgaga tacaacttgc
    30481 tgatgcagaa gaaccaatcg atacagtgat tgaaacgatt tgtgacaagc tgcttgcaaa
    30541 tgtaaatatg gaaacatacc gatatgaaat caaaaaaatg gaggaagtat aacatgaaat
    30601 ttgcggtcat cgtttttcca ggttcaaatt gtgacgcaga tatgttatgg gcagttcgcg
    30661 agatcatggg cgctgatgcc gaatttgtac ggcatgatga gtcctcttta gaaggatttg
    30721 atggggtttt actgccggga ggattctcct atggtgacta tttgagatgc ggtgcgattg
    30781 ctcggttttc tacaatcatg gaagaagtga ttcgttttgc tgaagaaggg aaaccagtat
    30841 tcggtacatg caatggattc cagatattga cagaagcagg acttttgcca ggtgtactga
    30901 gacgaaatga gtctttgcat tttatttgca aaactgtacc attgaaagtc aaccccaaga
    30961 cggcctttac ttcagagtat gaagccgacg aaataatcta tcttccagtt gctcatgggg
    31021 aaggaaatta tttctgtgac gaagaaacac ttcaagaact aaaaaaaaat aatcagattg
    31081 cctttactta taatgacaac atcaacggca gcgtggaaaa cattgcaggg attacgaaca
    31141 aagcagggaa tgtgttagga atgatgcctc atccagaacg tgcagtagaa gacttgctag
    31201 gatcagaaga tgggaaacgg ttctttgctt ccattttaaa aaacttcgga aaggtgaccg
    31261 tatagatgaa aacattgaaa gaaccgacag ctgaagagat caagaaaaaa agaatctatg
    31321 ccgaatgggg attgactgat gaagaatatc gaatgatcga agaagatatc ttaggaagaa
    31381 tgcctaatta tacggaaact ggactgtttt ctgttatgtg gagcgaacat tgttcctata
    31441 aaaattctaa accggttcta cgcaaatttc ctactagtgg acctcatgtg ttgcaaggcc
    31501 ctggagaagg cgcaggcatc gtcgacatcg gtgatggact agcagttgtc ttcaaagcag
    31561 aaagtcacaa tcatccttca gccgtggagc cttatgaagg agctgcgaca ggagttggcg
    31621 gaatcatcag agatattttc agcatggggg ctcgtccaat tgcgttgcta gattcattga
    31681 ggttcggcga attagataat ccaagaacga aatatttact agaagaagta gttgcaggta
    31741 tcggcggata cggcaattgt atcggcatac cgactgtagg cggcgaaatt gcatttgatc
    31801 cttgttatga aggaaaccca ttagtcaatg caatgtgcgt aggtttgatt gaacacaaac
    31861 atatccagaa agggcaagcc gctggcgtcg gtaattcgat catgtatgta ggtgcaaaaa
    31921 caggtcgcga cggtatccac ggagcaactt ttgcctcaga agaatttaat caagaagaag
    31981 aacagcaacg ttctgctgta caagtaggcg atccttttat ggaaaagctg ctgcttgaag
    32041 cgtgtttgga attaatcttg catcattcgg attctctaat aggtatacag gatatgggag
    32101 cagcaggact tgtttcatct agtgcggaaa tggcttcaaa agctggctca ggacttatat
    32161 tgacattaga tgaagtccca caaagagaaa ctgggatgac accttatgaa atgatgttat
    32221 cggaatcaca agaacggatg ttgatttgtg ttaaatcagg agaggaagaa aaaatccaag
    32281 aactatttca gaagtatgat ctagatgcag tcactatcgg taaagtaaca gacgacgggc
    32341 aatatcggct atatcatcaa ggagaagaag tagcaaatct gccagtcgat gcattagctg
    32401 aagatgcacc agtttatcat aaagaaatga aagaaccagc tcgaatcgta gaatttcagc
    32461 agttagctcc ttatcagcca gttatcgaag aacctggaca agtattgctt gatctgctgc
    32521 agcaaccgac gattgcttca aaacgttcaa tctacgaaac ttatgattca caagtacaaa
    32581 ccaatacagt tgtccgtcca ggaagcgatg ctgctgtgat gagggttaga gggacgaata
    32641 aagcgttagc tatgaccaca gactgcaacg cgcgctatct gtatttggat ccaaaaatcg
    32701 gaggacaaat agcagtggca gaagctgcta gaaatattat tgctagcggt ggtaagccct
    32761 tggcgatcac cgactgcctg aactatggtt ctcctgataa accagaagtt ttttgggaat
    32821 tatcgacttc agctgacgga attgctgctg cgtgtgaaac gttaggaact cctgttattt
    32881 caggaaatgt ctctttatat aatgaaacgg atggtcaagc aatctatcca acaccaatga
    32941 ttggtatggt cggattgatt gaggatcata aacatatcac tactcaggaa ttcaaaaaat
    33001 caggggatct aatctatata ttaggtaaga cgtttgcaga ttttgatggc agtgaattac
    33061 aaaagatgca gcttggaaga atcgaaggtg tgatccgaaa ctttgatcta tccattgaaa
    33121 agagaaacca agagttagta ttgacagcca tccaaaacgg attgattgaa agtgctcatg
    33181 actgttcaga aggtggcttg gcaattgcac tggcagaatc agctttcaaa catcagctag
    33241 ggatatctgt acagtttgaa ttgtcctctg ctcaattatt tgcagaaact caatcaagat
    33301 ttgtgttgac agtcgcaccg gaaaataaga cacgattcga agaaatgatg ggagatgctg
    33361 ctgttttagc aggtaaggtg acggatgaag cgataatcga aatttcagca actgacggtc
    33421 agatcaaaat agaaactgca gtagcaagaa agtgctggga ggatgcaatc gtatgtctta
    33481 tgaagtgaag agtttaaatg aagaatgcgg gatcttcggt gtttggggtc atccagacgc
    33541 cgctagtgtg gcatacttcg ggctccatag tcttcagcat cgaggacaag aaggtgcagg
    33601 tatcgtgtcc aatgatcgag gaaagcttaa ggggcatcga gatttggggc tgctttctga
    33661 ggtattcaaa gatcaaagaa aattgcagca gttaacagga gaagctgcaa tcggacatgt
    33721 acgttatgca acagcaggca atggaagtgt ggacaatatt cagccttttc tattcaaatt
    33781 tcatgatcag tcgatcggct tagcgcataa tggtaacttg atcaatgctc gttcccttag
    33841 aaaagagtta gaggaaaatg gggcgatctt ccattctaac tccgatacgg agatattgat
    33901 gcatttgatc cgacgaagtg aagaaacaga ttttttaaat aaattaaaaa gtgctttgaa
    33961 tgaagtcaaa ggtggctttg cctatttatt aatgacagaa acggcaatga ttgccgcttt
    34021 ggacccaaat ggttttcgtc cattagcaat cggacaaatg aaaaacggag catacgtgat
    34081 tgcctcggaa acttgtgcat tagaagttat tggggcacgt tttataagag atgtccagcc
    34141 tggtgagata gtgatcatca atgacgaagg aatccagatt gattgcttta cgaaggacac
    34201 gcagctttct atttgttcga tggaatatat ctattttgcc agaccagatt caaacatcgc
    34261 agggatcaat gtgcatactg cacggaaaaa tatgggacgg aatttagcga aagaatcgcc
    34321 agtaaaagca gatatggtcg ttggcgtacc taattcttct ttgtcagccg caagcggtta
    34381 tgcggaagct agtggaatcc cttatgaaat cggattagta aaaaatcaat acattgcccg
    34441 aacatttatc cagccaacgc ctgagctaag agagcaaggt gttcgaatga aattatctgc
    34501 tgtacgagga gtagtggaag gtaaaaaggt gattatggtc gatgattcaa ttgttcgggg
    34561 tacgacaagc agacggatcg tccatctttt gaaagaagca ggggcaaaag aagtccatgt
    34621 gaggattgct tctcctcctt tgaaatatcc atgtttttac gggattgata ttcaaacgcg
    34681 taaagaactc attgcggcaa atcacacgat tgatgaaatc aaagaatgta tcggtgcaga
    34741 ttctttaggt tttttaagtg aagagggtct gatcgaatcg atcggtctgg aaaaagatgc
    34801 tccgtattca ggattgtgta tggcttattt caacggagat tatccgacgc cgttatatga
    34861 ctatgaagaa aaatatcagg aatcactaaa agaaaaagta tcgttctttt aaaagcagac
    34921 caagaaggga gtatatgaat tcaatggcaa atgcatacgc aaaagctggc gtagatgtcg
    34981 aagctggata tgaagtagta gaacggatca agaagcatgt caaaaaaacg gaacgtttag
    35041 gtatgatggg cgctatagga ggattcggcg gttgttttga tttatcggtc tttcaattaa
    35101 aagagccagt ccttgtctcg gggaccgatg gcgtggggac gaaattagca gtggcaatcg
    35161 aagcagacaa acacgataca atcgggatcg actgcgtagc gatgtgtgtc aatgatatct
    35221 tagcacaagg cgcagagccg ttatatttct tagactactt agcaacgggg aaaaatgatc
    35281 cgttacgttt ggaaaatgta gtaaaaggtg tagcagatgg atgtgttcaa ggaaatatgg
    35341 cgctgattgg cggagaaaca gctgaaatgc ctggtatgta ctcaggtaat gattatgatc
    35401 tagccggatt tgctgtaggg atagctgaaa aatctcaatt attgacagga gaaacgatca
    35461 acccaggaga tgtgttgctt ggaatagcat ctagcgggat tcattcaaat gggtactctc
    35521 ttgttcgcaa aattttcttt gagcagcagc agttttcttt atcatcaaaa ctgccagaat
    35581 tgaaaaaaac acttggcgaa gaattgcttg agccaacgaa aatctatgtt tcttccttgc
    35641 ttccgctgat caaagaaaag aaaataaaag gagctgcaca tatcactggt ggagggtttg
    35701 tagaaaatat cccgcgaatg ttaccagaat ctatggctgc acaaatcaaa cttggctcat
    35761 ggccgattct tccaatattc ggctgtttac aaaaatatgg tgaaattcct gagatggaga
    35821 tgtatgaaat cttcaatatg ggcatcggta tggtcctggc agtcgaagaa tcgaaagtaa
    35881 aagaaatagt acacgaaatt gagcaagctg gcgaaaaagt ttatgaaatc gggaaagtga
    35941 tcgccaaaga aaaacatcgt gtggtgttta taggagaaga acgatgagaa tcgctgtttt
    36001 tgcttctgga aacggaagca actttcaagc gctagccgat tatctatcga aaaaaggatt
    36061 ggaatcttcg attgactggt tgttttgtga tcagcctgaa gcatatgttt tgaaacgtgc
    36121 aactgcgttg agtgtaccgg cagattgttt ttcacctaaa gaatttgatt caaaaaaaga
    36181 gtatgaagaa gcaattttgc ataaattgaa agaaaaaaag atcgatttga tcgtattagc
    36241 tggatatatg cgaatcattg gaccggtatt gctgaaaaat tatgataaaa gaatcatcaa
    36301 tatccatccg tctcttttac ctgccttccc tggtttgcat ggcatacgtg atgcctttga
    36361 agctggagta aaagaaacgg gtgtcacaat tcattatatc gaccaaggtg tagatactgg
    36421 accaatcatc cggcaggaaa aagtgagaat tgaacaggag gacacgttcg attctttaga
    36481 agaaaaaatc catagagtag agcatcgaat ctatccagaa gtgattagtg aaattattga
    36541 aaatggaggc tatctgaaat gacaagagca ttgatcagtg tatcagataa aactgggatt
    36601 gtagcattcg cccaaggttt gattgaacaa ggagtagaaa tcatttcaac aggaggaaca
    36661 aagcatgcgt tggataaagc tgggatcaaa acgctttcga tcgaagaagt gacagggttt
    36721 cctgaaatga tggatggccg tgtcaaaaca cttcatccta aaatccatgg tggtctttta
    36781 ggaagacgcg atctccctag ccatatggag gcaatggaaa cggaaaatat ccaaccaatc
    36841 gactttgttt gtgtcaattt atatcctttc aaagaaacga ttatgaaacc agatgtcgaa
    36901 atagcagaag ctattgaaaa cattgatatc ggtggtccta gtatgctccg tagtgcagca
    36961 aaaaaccatg aatttgtcac ctctgttgtt gatccatctg actatgaaga agtactttca
    37021 gaattacgtc agtatgggca tacttcactt tctttcagac aaaaactagc ggcaaaagtc
    37081 tttcgtcata ctgcggcata tgatgcactg atcgctcaat acttaacgga ttgggtggaa
    37141 gaaaaagaac cggaaaaatt gacacttacg tatgaacgaa aacaaacgct tcgttacggt
    37201 gaaaatagcc atcaagccgc tgccttttat cagtccgctc ttctggaaaa atattcgatc
    37261 gcagcagcaa aacaactcca tgggaaagaa ctttcttata ataatatccg agatgcagat
    37321 gctgcgctac ggatcatgaa ggaatatgac gagccaacgg tagtcgcctt gaaacacatg
    37381 aatccttgtg gtattgggac agcatctacg attttagaag cgtgggaagc agcgtatgaa
    37441 gcagatcctg tttcaatctt tggtgggatc atcgttttga atcgagaagt agatctgcca
    37501 acagcagaac agatgcataa gctgtttcta gaaatcatca ttgccccttc ttatacacaa
    37561 gaagctttgg aattactgca aaaaaagaaa aacatccgat tactgacagt agactttgcg
    37621 caggaagaaa atcaaaatcg agaagaaacg gtctcagtct taggtggact tcttgtacaa
    37681 gatcaagatc tggcaataga aaatgaagaa gagtggaaag tcgtgacaaa acgtaagccg
    37741 actccagcag aagcaaaagc attagcgttt gcttggagag ctgtcaaaca tgtgaaatct
    37801 aatgcgatcg tactggcaaa cgaacgtcag acagtaggaa tcggagccgg gcagatgaat
    37861 cgagtcggtt cagtaaaaat tgcgattgaa caagcgcaag agaaaatgga aggtgcagtt
    37921 ttagctagcg atgcttattt tccaatggat gattcggttg aatatgcagc caaacatggt
    37981 ataaaagcaa tcattcagcc aggaggaagc atcaaagacc aagattctat tgatatggcg
    38041 aataaatacg atctagcaat ggtctttact gatgttcggc attttagaca ttagagagga
    38101 gtcggataaa tgaacatttt ggtgatcggt gcaggtggtc gtgaacatgc aataagtaaa
    38161 aaacttttag ccagtccgaa ggtggatacg gtgtattgtg cgccaggaaa tccggggatg
    38221 acaaaagatg gtatcaattg tgtcgctatc agtgagcgag atcattcagc gttaatccat
    38281 tttgttaaga aagaggctat tgattggagt atcgttggtc ctgaagttcc tttgttaaat
    38341 gggatcgtcg atgattttat ggcggaagga ttgaaaatct tcggtccgac aaaagcagca
    38401 gctctgatcg aaggttcgaa aagttttgct aaacaattaa tggatactta tcaaatcccg
    38461 acagctaaat ccagaagttt tgaatgttac gaaaaagcag cagcgtatat caaagaaaat
    38521 ggtgcgccaa tcgtagtgaa agcggacggc ttagcagctg ggaaaggtgt agtcgtggct
    38581 accacagtga aggaagcgct ggatgcggca gaacaaatgt tgaaccagca tcgatttggg
    38641 gatagtagca aaaaaatcgt cgtagaagag tttttggcag gagaagaatt ctcccttttg
    38701 gcgtttgtcc gtaataccaa ggtgtatccg atggtcattt cgcaagatca taagcgggca
    38761 tttgataacg ataaagggcc aaatacaggc ggaatgggtg cttattcacc agtaccgcaa
    38821 atttcaaaaa agatgattga aaaagctgtg gaagaagttt taaaaccagc ggctttggga
    38881 atgaaagaac taggacgctc tttcacgggt gttttatatg cgggtttgat tgctacgaag
    38941 gaaggaccaa aagtgatcga attcaatgcg cgtttcggtg atcctgaaac gcaagtcgtt
    39001 ctttctcggt tgaagagtga ctttgcgcaa gtaatcgatg atttattaga gaatcgtaca
    39061 gtagagttaa agtggcagca agaaggatac aatgtaggcg ttgtggttgc agctgccggt
    39121 taccccgaag aatatgagac aggaatggtt ctaccagatt ttctggaaga agagctttct
    39181 gtttactatg ctggagtttc ctcaaataac ggcaaactag ttggagcagg aggacgtctt
    39241 tatttagtag aagcatacgg tgagacgatc aaagaagcac agaaaaaaat ttacaccgcg
    39301 ttaatggata aaacgacaga tggaacattt tatcgtacag atattggaga aaaagctgcc
    39361 aatagcgaac tgatcgtgaa gtaaatcagc tgcttccatt tataaaaaat acaacaaaca
    39421 taatcggaag cgccgatttt agtgaagcct tttcataaag agtgtggtac acttttgttg
    39481 tgtatataaa taaggaggag ttcagatgaa aaaaattcaa ttaggtactt ctgatcagaa
    39541 agcagctgca gtgatcttag gctgtatgcg tatcaacaat gcggaagatc cagtaaaagt
    39601 gattgagaca gcttatgaaa acggcattga tttttttgac catgcagata tttatggaaa
    39661 cggtgaatgt gaagaaatct ttgcagatgc tctagcaaaa acatcaatca aacgagaaga
    39721 tctttttatt caaacaaaat gtggaatcgt tccaggtgtg atgtttgatt tttctaaaaa
    39781 acatatcatt gaatctgtag aaaaaagttt gacccgtctg aaaatggatt atgtggatgc
    39841 tttacttctt catcgtccag atacattggt agaaccagag gaagtagctg aagcgtttga
    39901 tgaattggaa cggtcaggaa aagttcgtta ttttggcgtc agcaatcaaa aaccaatgca
    39961 aatcgaattg ctgaaaaaat atgttcgtca accattagta gctaatcagc tgcaatttgg
    40021 cttgaaacat actggaatga tcgatcaagg gattcatgtc aatatgacag atgaagcaag
    40081 tatcgatcat gacggtagtg tacttgatta ttcccgattg catgatatga cgatccaagc
    40141 atggtcacca taccagtatg gattttttga aggtgtattt atcgggaacg aaaaattccc
    40201 tgaattaaac caaacattag aagaactagc agctaaatat caaacgacac caactggctt
    40261 ggcttctgct tggattttac gccatccagc caatatgcaa gtgatcgccg gtacgatgaa
    40321 cgtagggcga atcaaggaaa tcgctcaagc ttcagaaatt aatatgtctc gggaagattg
    40381 gtacgaattg taccgtgctg ctggaaacat tttaccttaa agattgtcat aagcaggtca
    40441 aaagggctat aaattaaaaa gacagaataa cttgaaagct gttttcagct tcgagatatt
    40501 ctgtcttttt ttattgcaaa tggaaagagt atcattgtac ggatttcgtt tattagttat
    40561 ttttgttttt aaggaaaagc tgtgtccgat attcttttgg agaaatccct tgttttttct
    40621 taaataaata actgaaatag ttagggtcat tataaccaat ttcaaaagca atatctgaaa
    40681 tacgatagtc tgattgctgc aataattttt ttgctaattc gattctttgt tctgtcagat
    40741 aatcgatgaa tgtttggtta aacgattgag aaaaaatcgt actgaaatga gagggactta
    40801 atgcaacttg ttgagcgacc caagttaagg acatatctgg atcggtaaaa ttttctttga
    40861 taaaatccaa tgcatggttc aaaattggct gatatttgtt ccttgaaggc tgtgagggca
    40921 gagcagttaa gaaagaaatg aacatataaa ctgtcgaacg gtaaagcgag tcgtcggcgc
    40981 tgattgctaa gcgttgactg gtggatgtgc aatgaaccat ttcttgcggg attttctttt
    41041 ctttcaggca gacatgctgg agtagcttag tcaagacaaa taaacgatat aatcgtgttc
    41101 gagttggtgt gccttgtggt ttcgtcaagt gttcgatgta cttagatact tcgtttttcg
    41161 ataatgaaga caaatcaagt gagaggtcaa agtaaaagac agaatcaact ggaagtttcg
    41221 cttcttgaat caaatcctca tagctgataa tttgttcttt aagggaactg gcggtttcaa
    41281 gtatcatctg cgtgatctca aaagatgcag gtaattcgct gattctttct gcgacaggac
    41341 caatggcgac agaaaaatta tttttttgtc ccttttctaa ttcatggacc aacgtttggg
    41401 ctacctgata actggtctcg atcacatttt cttcaagaga atcaactatt aaaaatttga
    41461 tgaatcgaga ggaaacagca gagaagatca cttgttgatt ctccctaaat aaaaacttca
    41521 aataatcact gagatgagcg taatcttcaa agtctgtact gacttggttt gctgctaata
    41581 atacggtcat ttttttccct gctaaagagc gattgaaaga ttcagcaagt ttcaatgttt
    41641 cactgatcgt aagtgagcct ttgaacaaac cgttcaagaa atgatttttt ttcaattcga
    41701 aaacaagatc agaagaagtg tgcgtttctt tttgctgcaa atctaaagca gttactgctt
    41761 tttctattgc ttgtttcaat tcgctacttt taatcggttt aagcagataa tcatctgcct
    41821 gtacctggat tgctcctttc atataatcga aatcgtcgaa tccactaata aatatgagac
    41881 gagtccaagg aagtagtttc cttgcttctt ttgaaaaagt caacccgtcc atgaaaggca
    41941 tttggatatc agtcagaatg atgtcgggct gtaagtcgat aattgaagac agtgccatct
    42001 caccatctcc agcttcgccg acataagtga ttggataata agaaccaagg acagttattt
    42061 gttcccgcaa gttattgcga atgaggtatt cgtcttcgat gataaaaact ttatacatgt
    42121 ttgtttcctc caattgtttt gggagataga gggatcatca gtgtgacagt tgttccttct
    42181 cggtatttgc tgtcaatcgt gataggtttt tgatcatttc cgtaataaag aaggagacgc
    42241 ttgtttacgt tgtaaagtcc gtagccgata ctgaaatctg aatcgatccc tttggctaac
    42301 tctttacgta tttctgccag tttttctgca gggatgccaa tcccgttgtc tataactttg
    42361 aattggatca tctcatcaat tttgcatgcg gtaatacgaa taaatccttt gtgacggacg
    42421 tacttcgttc catggtatac agcattttca atcaatggct gcaagatcat ttttaaaata
    42481 gaaacaggaa aaagtgtttc tggtatatcg atcttatagt caagcatatg gccgtatctt
    42541 atttttaaga tgactagata atcattgata tggctaattt ctttttcaag aggaatccag
    42601 tcttgtcctt tgcttaaaga cgtacgaaaa aagccggaaa gtgcatgggt catagtcaga
    42661 gcttcttcaa agcagtcttg ttcgatcaaa gaaataatgg catccaaact gttataaata
    42721 aaatggggag taatctgagc ttgcaataca cgaacttcac tttgtgctaa atggtattgt
    42781 ttcaaagcgt tctcccgtaa agactggttt agatcttccg ccattttatt catgctagcg
    42841 gaaagtgttt gtaattccag tgtttccggc agggttaaac gaaagccaag gttccctgaa
    42901 gaaagttgtt ctgacatctc gatcagctga ttgatcggtt cttggacctt tctctctaaa
    42961 aagcgacgat tctttttcgc atagaaaaaa atggtactaa gaactccaag ttccactatt
    43021 gttaatagaa tgagcgagcg gacaagtgtc gtattttttt tgcttgctaa gtcgatctcc
    43081 acacgaacaa aatcttgtaa gatatctgac agcagttgag tgacagaatc tacctgattg
    43141 atgatctctt cattggtatc cataaaatct tttgccaaga tattattgat cagctcgttt
    43201 tgataatctt ccagtgtatc gatcgtacgt agcgatacat ctaacgtact aatttcttta
    43261 agtgtatttg tattattttt gatctctcga atatcattcc taagttcttc taggatattt
    43321 tcctcattga actcatctac ggttacttgg ccagaaatca agtcccacat actttccaaa
    43381 actttttgat caaccttcaa agcaattgca tctgcttttt ggatatttgc gatcgtctgt
    43441 tgataaatca aaagctgttg cgaatataac agtatgctca gtagcaaagg gataacagca
    43501 aatagaagca tggctttgtt tgcttgggca aaaatctgtt ttatgcttaa attttttgtc
    43561 atgatacttc tccagtcaat aatttctggt tgggatgtta gctatattct gctgggaaaa
    43621 gacagtctcg gaaataaaaa tatctttagg taatgttttt cctgtttgaa tatttccaaa
    43681 ataacgagta acggtattgg ctacatacca gccgacatca ggtttacact cgactacaca
    43741 attgacgatt cctgagcgta gcttctcgat catatcttct tgtccatcaa tagttacaat
    43801 gatcaaatct tctccaggtt tgattgatgt ttcttttatc gcagctaaag caccgtgagt
    43861 catttcatca ctatggctgt ataatacatc gatttcggat aaatcatttg tttctaaata
    43921 tcgtttcatc tggtcttttc ctttcatccg aataaaatca ccgacaattc ggtgaacaat
    43981 agtcatcgga ggtttatggt cgatggttcc atcttttttt agggttggtt tacgattgat
    44041 tgattccaaa aatccttcgg agcgtagctt ggtcggtgtg gagttctctg aaccagcaag
    44101 ttctaggatt ctgatttcct gtttctttga atttgtgaag tgattgctga caaataaacc
    44161 cgcccgattt ccttctgctt tgaaactggg gccaatgtgg gtgacgaaaa gctcgggatc
    44221 attcgtttga atatgtctgt cgacaagtat gacagggatc cctgctcgct ttgcttcttt
    44281 taatacagag tcccacccgg attcttgcaa aggagtaaaa atgatcatat ccactttata
    44341 ggcaatgaac gtccgtatat cttggatttg ccgttcctga ttcatgtagc catttcggta
    44401 gagaacttgg taatcttctt tttcaagcgc agtgataatt gattcagtat gctttttccg
    44461 ccagctactc tccgttcctg attgcgaaaa accaacgact agtgggtcag gtgcttcctt
    44521 tgtattaggt gtacacccca tcatttggta agcgattaca acaagggcta aaaaaaataa
    44581 aacacgtttc actttaaatc actccatctg gatttgttag tactatctta tcagataaaa
    44641 gggaaaatca aagtcaaaag gaatcaatcg aaaaaaatcc aagaaactta aaaaaatcca
    44701 agacaaaaaa agtacaccta aaaaatttaa agtaaatccc ataaaaaaat caatgttaac
    44761 gctttcttta ttagctatac tcggtgtatc aattgaaatg ggggataaaa aatgaaaaac
    44821 aaatttagaa agattgttgg gactatcgct attttgaccg caagcattct attaagtgcg
    44881 tgtggcaatt caggatctgc agatgatagt acggggtatg taggaatcgc tatgccgacc
    44941 aaatcagcag aaagatggat cgctgatggg aacaacatgg tctcggaatt agaaaaactg
    45001 ggttataaaa cagatcttca atatggagaa gataaagtag aaaatcaagt agcacaaata
    45061 gaaaatatga ttacaaaagg ggtagataca cttgtgattg cttctatcga tggttcggct
    45121 ttaactgatg tgttagctaa agcaaaagaa gcagatatca aggtgatcgc ctatgatcgt
    45181 ttactgatga attcagaaaa tgtcgattat tacgcgactt ttgataattt tggagtaggt
    45241 gtatcgcaag cagcttatat tgaagagcac ttaggattga aagaaggaaa aggaccattc
    45301 acgattgaat tatttggtgg ctcgcctgac gacaacaacg cactaatcaa ttacaacgga
    45361 gtgatgtctg ttttacaacc atatatggac aacggtcagt tggtcgtgcc atctggacag
    45421 acgagtttca gtcaaatcgc tacgcttcgc tgggatggtt ctacggcgca agcaaggatg
    45481 gataatctgt taagcgcaaa ttatactgac caaacattgg atgctgtttt gtcgccatat
    45541 gatccaataa gtttaggaat catttcgtca ttaaaaggcg tggggtatgg gagtgaaagt
    45601 aaacctttac ctgtgattac tggacaagat gcgacagttg ctggcgttaa atcgatcatt
    45661 gccggggaac aaacacaaac gatctttaaa gacacacgta tcttagcaaa aaatacgatt
    45721 gaaatgatca aagcaatcag tgatggagaa gaggttccgg tcaatgatac tgaaacttat
    45781 gataatgggg tgaaaactgt ccctacgtat ttagcaaata cagtatctgt cgataaagat
    45841 aattatcaag cagagctgat cgacaccgat tattataaag aatccgatct gaaaaattaa
    45901 gaggaaccaa acaaggaact aaagagatct aagtcagaga gaggataaga attatggcgg
    45961 attgtatttt aaaaatggca gatatcacca aaaaattttc tggaatccga gcgttagatt
    46021 gcgtgaatct gatgattgaa cgtggggaaa tccatgcatt gtgcggagaa aacggagcgg
    46081 gaaaatcaac attgatgaat gtcttgagcg gcttgtatcc atatggctcc tatgacgggg
    46141 aaatctttta tgatggaaag ctgtgtcaat ttaaagattt gaaagatagt gaagaaaaag
    46201 gcatcgtcat cattcatcaa gaattagcac taagtcctta tctttcaata aaagagaata
    46261 tttttctcgg aaatgaacaa gcagaaagag gcgtgattga ttgggaactg acagaaaaga
    46321 aaacatatga actgctgaat aaagtcggtt tgtctatcga cccgaatctt ctagtatccc
    46381 agattggcgt agggcaacag caactagttg aaatagctaa agcattatca aaatccgtcc
    46441 gtctgttgat tttggatgag cctaccgctg cattgaatga agaagaaagc gcgaatttgc
    46501 tgagattgat ccgagagttg aaagagcaag gtgtaacctc catcattatt tcacataagc
    46561 tgaatgaaat cgtggatatc gcagatcgaa tcaccatttt acgggatgga cagacgattg
    46621 agacactaga aaatgaaatg atcagcgaag agcatattat tcgcgggatg gtgggcagag
    46681 atttagttga tcgttatcct gaaagaaaac cggatattgg agaagtttat tttaaagtag
    46741 aagactggac ggtctatcac cctcttgatc acgagcgaat cataaacgac cacatcaact
    46801 tgcatttgaa aaaaggagag attgtaggaa tagctggact gatgggagcg ggcagaactg
    46861 aattcgctat gagtctattt ggacattctt atggaagcaa gatatccgga cgtatattaa
    46921 aggaaggggc agagatatca gttaagaccg ttccttcagc cattgcaaac ggactcgctt
    46981 atgtctcgga ggaccgaaaa tctttaggac tgaatctgct gatggatatt cgggaaaacg
    47041 tgacgctgag cagtttggat aaaataagtc aaaaaggagt attagataaa gaaaaagaag
    47101 taactgaggc agaaaatttc cggaaaaaga tgcgtataaa aacaaactcc atccatcaaa
    47161 ttgtcagtag tcttagtggt gggaatcaac aaaaagtcgt tttatccaag tggctgatga
    47221 cacaacccga tattcttttt ttggatgagc caacccgtgg gatagatgtt ggggcaaaat
    47281 atgagatcta cacgattatc gaagaaatgg cagcaaatgg caaagctgtc tgtcttatct
    47341 cttctgaact tcctgaaata ttagggatgt gtgatcgaat ctatacgatg aacgaaggac
    47401 gtattacagg agaagtctcg cgagaagatg cagatcagga agtcttgatg aaattaatga
    47461 cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc ttacggataa aaaagagaaa
    47521 aaactaaatt tgaaaagtag agcagctgat ctttttggtc gctacagcat ggtgatcatt
    47581 ttagcgggac tacttattgc gtttcaattg atgacggatg gtattttctg gcgtccattg
    47641 aatataacta accttgttct gcaaaatagc catatattag tactagctgc agggatgtta
    47701 ttagttgtgc tcttaggtca tgtggatttg tcggtagggt cagtgatggc gtttgttggt
    47761 gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc cgtggatgac agttatcgca
    47821 tgtcttgcag taggcgggct gatcggcgca tggcaaggtt tttgggtagc ttatgcaggg
    47881 ataccggcgt ttatcgtgac tttagcagga ttgctgatgt ttcgtggact gacacaagtt
    47941 gttctaggag gccaatctct agcacctttt ccagctgttt tccaaaaaat gtctacgggt
    48001 tatttaccag acttaagcga aggaagcagc cttcacttct tgacattgat tttaggaatg
    48061 atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa gaaatcgtcg gaaaaatcat
    48121 ttatttgaag tggaatctcc aacagctttc ttggtaaagg cggtatttac agcagtcgtt
    48181 gtactaggaa taacttatat ctttgcctct tatcaagggt atccgattat tttgatcatt
    48241 cttggtttga tcgttgcagc atatgggttt ttgacaaata aaacagtagc aggtcgacag
    48301 atttatgcga cgggcggaaa tcgaaaagcg gctgaactat cagggatcaa aacaaagcga
    48361 atcacgttct gggtattcgt caacatggga gtcatggcag ctttagctgg attagtttta
    48421 gctgctcgat tgaatgcggc aacacctcaa gcaggtacat cgatggaact ggatgcaatg
    48481 gcggcagtat attttggtgg tgcttcgact tccggcggca ttggaacaat catgggtgcg
    48541 attgtcggtg gcttggtcat gggtgtattg aacaatggca tgtcgatttt aggcgtcggg
    48601 gtagactggc agcaggcaat caaaggattg atcttgcttt tagccgttgt gctagacttg
    48661 tataataaga aaaaacgaac aacgtaggag aacgccggtt attcagaaaa aagactgtga
    48721 caagacattt gtcacagtct ttttttcgct aaaagcgaac attcattgtt tcgtttgtat
    48781 agactaaaaa taatatatgt ttttttacca ttcgtataaa ttctcttttt gctcttgatt
    48841 tttttgctta attgagttaa attacatagg gcgctaaaaa aagccttgat taaattaata
    48901 atgttcgtgt tttgctggat gtgaacatca ggaggataat gtaatgaaag tatttgatta
    48961 cgaagatatt caattaattc ctaataaatg tatcgtaaat agccgttctg aatgtgatac
    49021 aacggtcaca ttaggtaaac atacattcaa aatgccagta gtgcctgcca acatgcagac
    49081 gattattgat gaaacaatcg cagaattctt agcggaaaat ggttatttct atatcatgca
    49141 tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa atgaaaaagc gtggattgat
    49201 tacctcgatc agtgttggcg tcaagaaaga ggaatattcc tttatagaga agttggcaga
    49261 agaatcgcta aatcctgatt atatcactat cgacatcgcg catggacatg ctaactcagt
    49321 tattgatatg attcagcata ttaaaaaata tttgcctgaa acttttgtga ttgccggtaa
    49381 tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg ggcgcagatg caacaaaagt
    49441 cgggattggt ccaggaaaag tatgtattac aaaaatcaag acaggctttg ggactggagg
    49501 ctggcaactg gcagctctta gatggtgtgc gaaagcagct cgcaagccga tcatcgctga
    49561 cggtgggatc agaacacatg gcgatattgc aaaatctgtc cgttttggtg cgacaatggt
    49621 catgattggt tcattgtttg ccggtcacga ggaatctcct ggcgaaacaa aagtcgaaaa
    49681 tggaatcgtg tataaagaat actttggttc tgcttcagaa ttccaaaaag gcgaaaaacg
    49741 aaatgttgaa ggaaagaaaa tctggatcca acacaaagga tctttgaaag atacactagt
    49801 ggaaatgcaa caagaccttc aatcatctat ttcttatgct ggaggaagag atctggaagc
    49861 cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt tttaatggag acacaatcta
    49921 aaaaataaat aaaatattag gatgttgtga aaaagttgtc ctgcattaag taataagaac
    49981 agccaaacaa aaatagttct ttatatgttt tgtttggctg gaattattat cgcagataca
    50041 agcttttgaa catcattatt cagaataagg gactgtgata agatttttgt cacagtccct
    50101 tatgttttgc aaaaaaagtt tttctttttt ctcgtaaatt cggtaaaatc aaaagaaaaa
    50161 atagacaata gaaaagagaa gcttatgtgg gaaaaagtag agaaaaaaca aggaccgatt
    50221 tatcgtcaaa ttatggaaca gattatgaga gaaatcgaaa acggaaggat caatcctgga
    50281 agtcaacttg aatcagaacg aaaactaggg caactttttg gtgtgaatcg ttccaccgtt
    50341 gtccatgcat tggaagagct tagggagctt ggggtactaa cgagcaaacg tggcagcgga
    50401 tggtttgtta atcagactga atgggggaaa ttcgctgttc caagaatcga ttggcggcaa
    50461 atgatctctc ctcgttatga gcagacggac tggtatgagc aaaaaataag agaagcaaag
    50521 cagatccaaa aaacatcgtt tcttgattta tacgctagcg agatgccaga tgagcttttg
    50581 ccgaattttg agtttccttc ttattctctt gaagaaatct tagcggaaga aaaagaaatg
    50641 gatcttttag gctatactcc gttacgtaac aaaatcaaac ggtatttgga aagtaaattt
    50701 gaattggcat tttcccccaa ccagcttctt gtgactggtg gcggtcagca agcaatcttt
    50761 ttgattttac aaacgattct ttcagctgga gaggggatag ctgtagagtc accttctttt
    50821 ttttaccgat tacctctttt caaagcttca ggaacgcgtt tatttgggat accgatggat
    50881 gaagaaggga tcgacttaga aaaactgaaa gaatcgatcc ataaaaataa attgaaagca
    50941 gtgcttatca acccgaattt tcaaaaccct acaggaaaag tgatgagtca aaaacgacga
    51001 gaaaagctag tagaattgtg tcgacagtat cggcttccaa ttattgaaga cgatgtattt
    51061 acggatcttt catttctaaa ccaagaagaa gtccttccta tacgaacgat tgatccggat
    51121 aatgtgttat acgtcggttc gttatctagg gttttaggaa aaacaacgaa aatcggttgg
    51181 gtgataggac ctgagacttt tatctcgcaa ctagctcaag cgcaagaaat gatggagttt
    51241 tcgatgaata tccttacaca aatcacagtg gctaatgtat ttaatgaagc aattgatttg
    51301 aaaatgcaag atgtaagaaa aaagttgaaa gaaaaaagcc gactgataag cgcttgggga
    51361 gattcacaat cgtttttcaa gcttcattca attttaggtg gttactatgc ctggattact
    51421 tgggatggga aaaaactaga ccccgaacta gcagaaaaag tagtagatat gggtcttgga
    51481 attgcaccaa gtctactttt tgggcaagat ataaatggaa tcagaatcaa ttacagtagg
    51541 ctggatgaaa accaaatacc gttgttttat tcaaagatga aaattctaga aaagtggtta
    51601 acagaataat ttcacaaagt aaatagatta aagaatataa cagcttggat ttaaatttca
    51661 agctgtttct tttttttcgg aaaaagaata cgatataaag aaactaagaa aaaaccatta
    51721 tttgtacgtc cgttcacgat gtgtaaataa aataaatgtt tgcaacaaaa ctgtataaca
    51781 ccaacgtttt taaatagaaa cagatggttg taagtttgat tagaaaatag aaaaaatgca
    51841 gatgaaaaat aaaacgcttt ccttattgtg attttattca ctaatgctgt taatatgcgg
    51901 attggcataa tttcctttca tcgtgaaaat ttgtgctttt agcttgtatt tctcataaat
    51961 ccatgataat ttattaatga agacggttaa atagtgatac agttttattc actgtttcaa
    52021 tcgcagaatg aagagatttt cttatagatg agagaggaat gtgtgacaaa tggcaaacaa
    52081 aaaagcaccg atcgacttcc aagcactgct agatgaagtc aatgcagatt tcccagtata
    52141 tcaagcactt gatcaagatg gaaacgtagt taacaaagat ttagtacctg atttatccga
    52201 tgatgaactc gttgaattaa tgactcatat ggtatggtct cgtgtattag accaacgttc
    52261 aacagcgctg aaccgccaag gacgcctagg cttttttgca ccaactgctg gacaagaggc
    52321 aagtcagctg gctagtgctt acgcatttga taaagaagat gttcttcttc caggttatcg
    52381 ggatgtacct caactagtaa aacatggttt acctttatct caagcttttt tatggtctcg
    52441 aggacatgct gcaggtaatt cttatccgga agaattgaaa gctctaccac cacaaattat
    52501 catcggtgca caatatgtac aagcagccgg tgttgcatta ggtttgaaga aacgaaacaa
    52561 gaaaaatgtc gtcttcactt atactggtga tggcggttca tcacaaggtg atttttacga
    52621 agcaatcaat ttcgctggcg cttaccatgc aaatgccgta ttttatatcc aaaacaacgg
    52681 gtatgcgatc tctacacctc gtgaaaaaca aactgcagca aaaacacttg cacaaaaagc
    52741 tgtcgcagct gggattcctg gtattcaagt cgatggaatg gatccgcttg cagtatatac
    52801 tgtatctaaa atggcacggg aatgggcgat cagtggaaat ggaccagtcc taatcgaaac
    52861 actgacttat cgttatggac cacatacact atctggtgac gatccaaccc gctaccgtac
    52921 aaaagacaca gatgacgatt ggcagaaaaa agatccattg atccgcttcc gtaaatattt
    52981 gacggaaaaa ggactttggt ctgaagaaaa agaagaacaa gtcatcgaag caactaaaga
    53041 agaaatcaaa gcagctattg ctgaagccga caaagtacca aaacaaaagg tttcagattt
    53101 cttgaaaaac atgttcgaag tatctccaca atcaatcaaa gaacaaattg caatttatga
    53161 agcgaaggag tcgaaataat catggcacaa aaaacaatga tccaagcaat cacagatgcc
    53221 ttagcacttg aactagaaaa tgacgagaat gtcgttgttt ttggtgaaga tgttgggaaa
    53281 aacggaggag ttttccgggc aactgaagga ttgcaggaaa agttcggaga agaccgagta
    53341 ttcgatactc ctttagctga atctggaatc gcaggattga gtttcggtct tgcgttagaa
    53401 ggatttcgtc cagttcctga aatccaattc tttggtttta ttttcgaagc aatggacgaa
    53461 gtagtagctc aaatggctcg tacaagatat cgaatgagcg gcactagaaa tctacccatc
    53521 acaattcgtt cgccatttgg tggcggtgtt catacaccag aattgcattc ggataattta
    53581 gaaggtttga tcgctcagtc tcctggtatt cgagtagtga ttccttccaa cccttatgat
    53641 gcaaaaggac tattgatttc ttctattaga agtaacgatc cggttgtttt cctagaacac
    53701 atgaaactat atcgctcatt ccgagaagaa gtgccggatg aagcttacga agttcctttg
    53761 gataaagcag cagttactcg tgagggaaca gatgtatcta ttatcacata cggagcaatg
    53821 gtgcgtgaag caatcaaagc agcagacaat cttgcaaaag aaaatatatc tgttgaaatc
    53881 attgatcttc gtacagttgc gccattagat gtagaaacga tcattcaatc cgtggaaaaa
    53941 actggtcgcg tagttgttgt ccaagaagca caacgacaag cgggcgttgc cgcgcaagta
    54001 gtttctgaga tctcagaacg cgctatcctt tcattagaag ccccaatcgg acgagtttct
    54061 gcacctgata cagtcttccc atttggacaa gcagaaaatg tctggttgcc aaatgcaaaa
    54121 gatatcgaag acaaagtaaa agaaatcgcg gaattttaag aaaagaaggg acgaaacaaa
    54181 gtcatggctt atcaatttaa attgccggat atcggtgaag gaatcgcaga aggtgaaatc
    54241 gtcaaatggt tcgtcaaacc aggagatacg atcaatgaag atgatacatt actagaagtt
    54301 caaaatgaca aatcagtaga agaaatcccg tcacctgtta caggaacggt taaaaatgtg
    54361 attgttccag aaggtaccgt agcaaatgtt ggggatgtat tagtagaaat cgatgcacca
    54421 ggacacgaag acaatgaagg ggacagtggt gtagccgcag aatcccaaac tccagctaaa
    54481 ccggctgctg aacccactgt tgatacagaa tcagctggat cgtcaagcga aggagtcttc
    54541 caatttaagt taccggatat cggtgaagga atcgcagaag gtgaaatcgt caaatggttc
    54601 gtcaaaccag gagatacgat caatgaagat gatacattac tagaagttca aaatgacaaa
    54661 tcagtagaag aaattccgtc acctgtcaca gggacagtta aaaacgtgat tgttccagaa
    54721 ggcactgtag caaatgttgg ggatgtatta gtagaaatcg atgctcctgg acacaatagt
    54781 gcaccgtcaa ctagtgcgcc atcagctgaa gcaccaaaag aaaaagtgga aacatcaggt
    54841 tcagcaagtg tcgttgaagc tgccgatcca aataaacgtg tattggctat gccatctgtt
    54901 cgtcaatttg cccgtgaaaa agatgtagat atctcacaag taactgcaac tggaaaaggt
    54961 ggcagagtaa caaaagagga catcgaaaac ttcttggcag gtggtccaag cagtgcacca
    55021 gcgaaatcag aagcgccaga agcggcagca ccgaaagaag cagctcctgc agcagaaagc
    55081 aaaccagctg caccagcaaa accattcaag tccaacttag gagacttgga agaacgtgta
    55141 gcaatgacgc caacacgtaa agctatcgct aaagcaatgg tcaacagtaa gcatacagct
    55201 ccacacgtga cattgcatga tgaagtagaa gtgtctaaac tatgggataa tcggaaacga
    55261 tttaaagaag ttgctgctgc aaatggtacg aaacttactt tcttgccata tgtcgtgaaa
    55321 gctttaacag cgactgttaa gaaataccca gtattgaatg catctatcga cgatgcaaac
    55381 caagaaatcg tttataaaca ttactacaat atcggtattg caactgatac ggatcacgga
    55441 ctatatgtac caaatgtgaa agatgcagac cgcaaaggaa tgtttgctat tgcagacgaa
    55501 atcaatgaga aagcaaaatt agctcatgac ggcaaactat ctgcagaaga tatgcgcaat
    55561 ggaacaatta cgatcagcaa tatcggatct gtaggtggcg gttggtttac accagtcatc
    55621 aactatccag aagtagctat tttaggtgta ggaacgattg cacaacaacc aatcgtaaat
    55681 gctgaaggcg aaatcgttgt cggtcgtgtg atgaaactgt cattaagttt cgatcaccgt
    55741 atcgtagatg gagcaactgc gcaacaagca atgaataata tcaaacgtct tttagctgat
    55801 ccagagctat taatgatgga aggatgataa acaaatggta gttggagatt ttgctgtaga
    55861 attagataca gttgtaattg gtgcgggacc tggtggctat gtagctgcca tccgcgctgc
    55921 tgaaatggga caaaaagtag caatcattga aagagagtat attggcggtg tttgtttgaa
    55981 tgtcggctgt attccttcaa aagcactgat agctgcagga caccattatc aagagtcact
    56041 agattctgaa atgtttggag taactagtga aaacgtcaaa ctagattttg caaaaacaca
    56101 agaatggaaa gaaaacaaag ttgtccatac gttgacttct ggtgttggtt tcctattaaa
    56161 aaaacataag gttgaaacaa tcgaaggaga agcgttcttc gtagatgatc acacactacg
    56221 cgtcattcat ccagattcag ctcaaactta ttcgttcaat aacgcaatca tcgcaacagg
    56281 ttcccgtcca atcgagattc caggatttaa attcggagga cgtgtgttag attctactgg
    56341 cggattagca ttgaaagaag tacctaagaa atttgtgatc atcggtggcg gtgtgattgg
    56401 tgccgaattg ggcggagcgt acgcaaactt aggagcagaa gtaacgattc ttgaaggctc
    56461 accacaaatt ttaccaactt acgaaaaaga tctagtaaaa ttagtagaag atgatttcaa
    56521 gaaaaaaggc gtaacagtcg taacgaatgc aatggcgaaa gaagcagtcg ataacggtga
    56581 cagcgtgaca gtgaaatatg cagtggacgg gaaagaagaa tccgttactg ctgattatgt
    56641 gatggtcact gtaggtcgtc gtccgaatac agatgatatg ggcttagaac aagcgggtgt
    56701 cgaagtagga gaacgaggat tgatcacggt ggataaacaa ggacgtacga acgtacctaa
    56761 catctatgcc atcggtgata tcgttccagg ggcggcatta gcacacaaag ccagttatga
    56821 agcaaaaatc gctgctgaag caatctctgg caaaaaagta gctgtagatt ataaagcaat
    56881 gccggcagta gccttcactg atcctgaatt ggcttctgta ggtatgacga tcaaagaagc
    56941 aaaagatgct ggtatcgaag caaaagcata taaattccca ttttcaggga acggtcgggc
    57001 attatcgctt ggtaaaacag aaggattcat ccgtctagtt acgacgatcg aagataacgt
    57061 gcttattggc gcgcaaatcg gtggagtcgg cgcaagtgat atggtctctg aattagcttt
    57121 agcaatcgaa tcaggaatga atgctgaaga tatcgccttg acgattcatc ctcatccatc
    57181 tcttggcgaa atcgtcatgg atgcttcgga attagcatta ggtttaccaa tccatatttg
    57241 atttgaataa tacaggcgaa acgcaactga aaaatcagtt gcgttttgtt ttttagaatt
    57301 atcaataata ttattatgta aacttaaaaa tatgacaact tcttggttta ttaccacttc
    57361 ctatgctaca ctgaagagga atatgaaata ggagagttga agatgcaaga atatcagtat
    57421 ccacttgatt tagattggac aacagacgaa atggtcattg taatgaagat gtgggaagat
    57481 ctggaacgtg cgaatgaaaa agggataaac aatgaagctt ttttgaagac ttatcagcag
    57541 tttaaaactg ttgtcaaatc cattggtgag gaaaaacgat tgggacggga atttgaaaaa
    57601 gcttccggtt attctttata tcgtacagtg cagcaagcaa aaaaaaatcc aaacaaaact
    57661 ttgagaatga acgggtgaga taaatggaac agatgattga tcatatcaag tcctggcttg
    57721 aagaagctgg tgagaaaatc aaatcttctc aacaaacatt aacaatctcg caaaaatcaa
    57781 atcgaacgga tctagtaaca aatgtcgatc gaaacatcca gaattttcta atcgaaaaaa
    57841 tccattcctt tgatccagag gcgaaaatat tggcagaaga agaagggtgt aatcaattat
    57901 cggatctcga tggaagagtc tttatcatcg atcctattga tggcaccttg aattttgtga
    57961 ttgaagggga aaatttttgt attatgctcg ctgtttatga atccggcgtt ggaaagcttg
    58021 gtttcattta tgacgtgatg agaaatgaac ttttttgggg tggaaaagga ataggcgtgt
    58081 ataaaaatga tgagccattg cctagaccag caaataaatc cttatcggaa ggcttacttg
    58141 gtgtgaatag ttatcttttc ggtcataata aatatggaat ccgagaaatt gcggaaagca
    58201 gcatggggac acgtatgtac ggatgcgctg gtcttgaact agtggcaatc ttgaaaggaa
    58261 accacattgc ttatatttca aaccttagcc cttgggatta tgcagcggga aatgtcttat
    58321 tggaagagtt tggtatggta acaagcggtc tttttggtac tcctttgaca tttgaaaaaa
    58381 gggaatacta tttagcaggg acttcttgtg catatgcaga aatcgcagac cttttgaaaa
    58441 aagaaggctg aaaaaaaggt agaaatcatt cacaaaaatg ataaaaacga gcaaaagatt
    58501 aattttctcg aaaaaatgaa aacattttac tatatttcat gaaaaaagtc tgctataatt
    58561 agcagtcgag taaaatttgc cctgtcataa agaaccaata aaaaaggagt tagttacatt
    58621 gaattataga aacgatatcc gtaacgtcgc aattattgcc cacgttgacc acggaaaaac
    58681 aacattagtt gatgaactat taaaacaatc agatacatta gacgcacata cgcaattgca
    58741 agaacgtgcg atggactcaa atgcgctaga aaaagaacgt ggtatcacga ttttagccaa
    58801 aaacacagct gtagactata aaggtattcg cgtaaacatc atggatactc caggacacgc
    58861 ggacttcggt ggcgaagtag aacggatcat gaaaatggtt gacggcgttg tgttggtcgt
    58921 agatgcgtat gaaggaacaa tgcctcaaac acgttttgta ttgaaaaaag cactggaaca
    58981 acatatcaca ccaatcgttg tcgtaaacaa gattgacaaa ccttctgctc gtcctgagca
    59041 tgtcgtagat gaagttcttg aactattcat cgaattagga gcagatgatg atcagttaga
    59101 ctttcctgtg atttatgcat ctgcactaaa tggtacgtca agcttgtcag atgatccagc
    59161 agatcaagaa ccaaccatgg cacctatttt tgatacaatt attgaaaaaa tccctgctcc
    59221 tgtcgataac agcgacgaac cattacaatt ccaagtttca ttacttgact acaacgatta
    59281 tgtaggtcgt atcggaatcg gacgtgtatt tagaggaaca atcaaagtag gagaccaagt
    59341 agcattaatt aaattagacg gaacagttaa gaaattccgt gtaacgaaat tatttggttt
    59401 ctttggacta aaacgtctag agattcaaga agcaaaagct ggagacttga ttgctgtctc
    59461 aggtatggaa gatatctttg tcggtgaaac agttactcct gtggatcatc aagatgcgtt
    59521 acctatcctt cacattgacg aaccaacatt gcaaatgact ttcttagtta ataattcacc
    59581 atttgccggt cgagaaggta aattcgtaac cgctcgtaag attgaagaac gtttgatggc
    59641 agaacttcaa acagatgtat ccttacgagt tgaaccaaca aactctccag atgcatggac
    59701 agtttctggt cgtggagaat tgcatttgtc tatcttgatt gaaaacatgc gccgtgaggg
    59761 atacgaattg caagtatctc gtccagaagt tattgaaaaa gaaatcgacg gtgtaaaatg
    59821 cgaaccgttt gaacgcgtgc aaatcgatac accagaagaa tatatgggta gtgttatcga
    59881 gtccttgagc ttacgtaaag gtgaaatgca agatatggtc catacaggaa acggacaaat
    59941 ccgtttgaca ttcttaacac ctgcccgcgg tttgattggt tattcaactg aattcctttc
    60001 aatgacacgt ggttacggaa tcatgaacca tacttttgac caatatctgc caatgcttcc
    60061 tggtcaaatt ggcggacgtc accaaggggc gcttgtttcg atcgatacag gaaaagcaac
    60121 gacttattct atcatgagta tcgaagaacg cggtacagtc ttcgtagaac caggtacaga
    60181 agtatatgaa ggaatgatta tcggggaaaa cagccgtgat aatgatttga ctgtcaacat
    60241 cacgaaagca aaacaaatga caaacgtacg gtctgctact aaagaccaaa catctgtcat
    60301 caaaaaacca aaacaattga cgttggaaga atctttggaa ttcttgaatg atgatgaata
    60361 ttgcgaagta acaccagaaa gcattcgctt gagaaaacaa attttagaga aaaatgctcg
    60421 tgaaaaagca tctaaaaaga aaaaataatc tgtaaaagga tgaagagaga aaccagcttg
    60481 ttataaaaaa tagctggttt ctttttttgt ccaactcttt tctgtggaga agtcatcttt
    60541 gtcaatggac ataaattcag aaaatacgcc gaaagacgtt taaaagactt ttaaaaaact
    60601 tatttctaga gagtttttgt ttcataataa gataatgttt cacgaaagga gatcaacttg
    60661 tccttcgaag aaaatcgaag taaaatggca agagaaagga gcgggaagat gaacgactta
    60721 ttaaaacaat actttggtta tgatgagttt agacctggtc aaaaagaaat tatccagaaa
    60781 gtcattgatc aggaaaatgt attaggaatc atgcctaccg gaagtgggaa atcaatctgt
    60841 taccagcttc cagctttact attagatggg cttactgtgg ttgtgtcccc tttaatctct
    60901 ttgatgaagg atcaggtgga cgcggcgaat caattaggta tacccgccac ttttatcaat
    60961 agctcgttag atggttacga aacagctaga cgcttccaag aaatcgatag acagcagtac
    61021 cgtttacttt atatcgctcc tgaacgtttt atcatgccgg attttatcca agcaatgaag
    61081 cgatggaacg tccgtatgat cgctattgac gaggcacatt gtatctctca gtgggggcat
    61141 gatttccgcc caagttattt acagatggcg aaccagctag atcaactgcc aaatcgtcct
    61201 gtaattgttg ctttgacagc tacagcgact gtgcaagtag ctgctgacat aaaaagactt
    61261 ttaaaaattc cagaaaacaa ccatattcag actggttttg aacgagaaaa tctacgattt
    61321 caagtaatta aagatcaaaa aaaagaacaa tatctaatcg aatatttaaa gatcaacaaa
    61381 aatcaatctg gtatcattta tgcagcaacg agaaaagaag tagaccgtct ctatcatttg
    61441 cttaaaaaat tcgatttttc cgtgggtaga tatcatggtg gtctaaatga gaatgagcga
    61501 acagaaatgc aagaagcctt tttatacgat cgcttgcagc tgattgtagc gacaaatgca
    61561 tttggtatgg gtataaataa aagcaatgtc cgttttgtta tccattatca aattcctggc
    61621 tctctagaag catactatca ggaagctggt cgggcgggaa gagatggtct ttccagtgaa
    61681 gccatcctgc ttttctctcc tcaagatatc caagtccaaa agttttttat ccagcagtcc
    61741 caaagagaag aagggcaaaa gcaaaaagaa tatgaaaaat taaaagcgat gacagaatac
    61801 gtgtatattg agtcctgtct tcagcaatac atcctaaact attttggcga gacaagcagt
    61861 ccgtgcaacc gttgtggtaa ctgtttagat gacagagaaa tagtagaagt aacgacagaa
    61921 gcacagatgg ttctttcttg cttaaaacgt atgggtgaga actatggtaa acaaatgctg
    61981 atgaaagtct tagcaggatc gaaagaacaa aagctgcaag ctttaggttt tggacattta
    62041 tctacctatg gactgatgaa aaaccaatcg caaaaagaaa cgatgcagtt aattgaatat
    62101 ttgatatcga atggctatct tctgaccatc aatggtgagt atcctgtttt gaaagtaaca
    62161 gaaagaggga tacaagtatt gaaaggacaa gaatctgttt atcgaaagga acccaagaaa
    62221 gtccagcagc tatcggatga agaaaccgat acactatttg aagtgctgcg ggaactgcga
    62281 acggatctag catcagaagc aggcgttccc ccgtatgtcg tattttcaga ttccacattg
    62341 aaagaaatga gccgaatacg tccatcaagc agattggaga tgcttcagat aaaaggagtt
    62401 ggccaaagca agcttgataa atatggcgaa gcctttttat ctcggataaa aaatgcggac
    62461 tctaacgttc tggataagta gaaagaacga atacgtaaaa atgaataaaa acaactggtt
    62521 aaatctgttt tttttgctcc ttatgaaaat caaatatgtt ataattaagg aattagaatg
    62581 ggaggtaagc acagatggat tctttgtcga aaaaacttgc gatccaagta ttaaatgatg
    62641 aagcagaacg gatcaaacga ttaatccgaa atcaaaacaa cagtctctgt atctcacaat
    62701 gcaaagcctt cgaagaagtg gtagatacac agatgtacgg cttttcccgg caagtcggtt
    62761 ttgctgtccg tataggagta atcggaaaga acgagggcca gctgatgtta agtgagctag
    62821 aacgagaatt gaatcagtta tatagtgagg tttaccaaga aatttatgat aaaaaagaag
    62881 ttggcaagga ggaatagact tgcctaagaa agtgaaaaaa aggcatctat tagactatag
    62941 catcttgatt ccctatttaa ttttatgtgt attgggattg atcatggtat atagttctac
    63001 gtcgtatcta ttattagaaa acggacaaaa tccgtccgca tcagtcatta accagagtat
    63061 tttttgggtg cttagcttga ttgctatcgc actactttat aaaatgaaaa cagatgtttt
    63121 gaaaaaccag cgtttgatca tggcagccat cgcagttttg acgattttgc tgctgattgt
    63181 tgtgtttttt ggtaaggaga tcaatggggc gaaaggttgg ctgcaaattg caggattctc
    63241 gatccaacct gccgaatacc taaaaatcat ttctatctgg tacctgtctc tgactttatc
    63301 taaaagacaa aacagtgtac aaaaagattt tcttgggact gtgaaacgtc cacttgcaat
    63361 ggttatcgga cttactgcat tagtcgctat tcttcccgat tttggtaatg cggcagttat
    63421 ctttttgatt attttggttc tgcttttagc cagcggagtc aattatgtgt atactttgat
    63481 tgtaggagta ggaggctttt gtctaagtac ttttacgatc tggctgatca atatcacaaa
    63541 cggaaagatc ttacccggac gtttacagta tatctataat cgatttgcta tttaccaaaa
    63601 tcctttctca gacgaattaa ataaagggca tcaactagtc aacggctact atgccatgtt
    63661 caacggagga ctatttggca ggggattagg taacagtatc cagaaaaaag gatttttaca
    63721 agaagcacaa accgatttta tttatgcaat cgtagtggaa gaactgggag tgattatggg
    63781 gattttgata ttagcgttgc tattctttat gattgtgcgc attgtccttg taggtatccg
    63841 ttcaaaagat ccgttcaatt cgcttttgtg cattgggatc ggcgcaatgt ttttgatcca
    63901 agtatttgtc aatcttggag gaattactgg cgttattcct ttgacaggga tcacgttccc
    63961 atttttaagt caaggaggct ccagtttgct gatgctttcg atttgtgtcg gctttgtcct
    64021 caacatcagt gcggatgaaa aacgcaagag cttaggttta taataaaaaa agatgtagga
    64081 gtggttcgat gaaaaaagtt ttagttgcta acaggggtga aatcgctgta cgtgtatttc
    64141 gtgcttgtac agaattagga attaaaactg ttggaatcta tgcagaagaa gatgaatatt
    64201 ccgtacaccg tttcaaagcg gatgaagcat atttagtagg acaaggaaaa aaaccgattg
    64261 atgcgtatct agatattgaa gggatcattt ctatcgcgaa agaatgcgga gctgacgcta
    64321 ttcatccggg ttacggtctt ttgtcggaaa acttgaattt tgcgcaacgg tgcgaagaag
    64381 aaggcatcat ttttgttgga cccaaactgc atcatttaga tatttttgga gataaaatca
    64441 aagcaaaagc tgcagctatc gaagcgggga ttgcttcgat tccaggcaca gatggaccaa
    64501 tcgcttctat agacgatgcc ttggagtttg cgaaacaata cggttatccg attatgatca
    64561 aggcagctct aggcggcggc ggacgcggca tgcgtgtagc tcacgatgaa aagagtgcaa
    64621 gagagggcta cgaacgtgcg aaaagcgaag caaaagcagc ctttggtagt gatgaagtgt
    64681 atgttgaaaa atacatcgct aatcctaagc atatcgaagt acagattctc ggggatacgc
    64741 atggaaacgt catccatctt tttgaaagag attgttctgt tcagcgccgt caccaaaaag
    64801 tagtagaagt agcgccttgt gtctctatga atgaacagca acgtcaaaaa atctgccagg
    64861 cagctgtcca attgatgaaa tatgtcgggt atgtcaacgc aggtacagta gaatttcttg
    64921 ttgaaggaga tgatttttac tttatcgaag tcaaccctcg cgtccaagtg gaacatacga
    64981 tcacagaaat gattacagat attgatatcg taacaacgca actgttgatt gctcaaggtt
    65041 tggatctgca taaagaaatc ggattgcctc agcaagaagg aattaaatta aatggttcag
    65101 ccattcaatg ccgaatcact acagaagacc cgttgaataa ctttttacca gacacaggga
    65161 aaatcgatac gtatcgttca cctggtggtt ttggcgtacg tctggatgtc ggaaatgctt
    65221 acgcgggcta tgtagtgaca ccgtattttg attcattgct agtaaaagta tgtacgcatg
    65281 gtgctacctt tgaaacagcc attcagaaaa tggaacgttg cttgaaagaa tttagaatcc
    65341 gtggagtgaa aacaaacatt ccatttatgc tcaatgtcat cacacatcct gaattccaat
    65401 caggaaatgc aaaaacaacc ttcattgata gtacagcaac attatttgaa tttcctcgac
    65461 tacgagaccg cggaaacaaa acgatgaaat atatcggtga aattactgtg aatggttttc
    65521 caggtatcga aagtggcgaa aaaccatttt atgaagaacc acgtatgcct aaagatctaa
    65581 tcacaagaac tgattacgta acggctaaga atgtattaga tgcgaagggt gccgatgctt
    65641 tggtcgaatg gatcaaagga caggaaaatt tgttgttgac ggatacgaca tttagagacg
    65701 cccatcaaag tctgctagca actcgtgttc ggacaaagga tttcaagcag attgcacgtt
    65761 tgactggaga aggtcttcct gaactctttt caagtgagat gtggggggga gcaacatttg
    65821 atgtggctta ccgctttttg aatgaagatc cttggcaacg gttgagaaaa attcgttctt
    65881 taatgcctaa tacgttgctg caaatgcttt tcagaggttc aaatgccgtt gggtattcta
    65941 attatcctga caatgtattg gtggaatttg tcaaagaagc agcagcgcaa ggaatcgatg
    66001 tatttcgaat ctttgatagt ttgaactgga ctccacaaat ggaaaaaagt atccaagctg
    66061 tacgggatac tggtaaaatc gcagaggcag ctatttgcta tactggcgat atcaacgatc
    66121 caagtcgggc aaaatacaat gttcagtatt ataaagacat ggcaaaggaa ctagaacaac
    66181 tgggtgcgca tatcattgca atcaaagata tggctgggct gcttaaaccg caggcagctt
    66241 atcgtttgat tagtgaattg aaagaaacga cagatctgcc aatccatctg catactcatg
    66301 ataccagcgg gaatggaatc atcacttatt ctgctgcttc aaaagcagga gtagacatcg
    66361 ttgacgtggc aatgagtgca atgagtggga atactagtca gcctagcatg agcagcttgt
    66421 attatgcact ggtcaatggt ccacggctgc cagaaatcac gattgaaaat gcacagaaat
    66481 taaaccatta ttgggaagat gtgcgcatgt attacaaacc gtttgaaaac ggcttgaatg
    66541 ctcctgaaac agaagtatat atgcatgaaa tgccaggagg acagtattcg aatctacagc
    66601 aacaggcaaa agctgtcgga ttaggtcatc gctgggatga aatcaaacaa atgtatcata
    66661 ctgtcaactt gatgtttggc gatatcgtta aagtaactcc atcatctaaa gtagttggag
    66721 atatggcact atttatggtc caaaatgatc ttacagaaga agatatttat gaaaaaggcg
    66781 aaacactaag tttccctgaa tctgttgtga ctttcttcca aggagaacta gggcaacccg
    66841 ttggcggatt tcctaaaaaa ttgcaaaaga ttattcttaa aggcagacct gcattgaatg
    66901 aacgtccagg tcttttggct gaatcagtcg acttcaacga agtaaaaaaa gaattggcag
    66961 aaaaaattgg ctatgaacca aaacaagacg aagtattaag ctatttgatg tatccgcaag
    67021 tctttttgga ctatcaaaaa gcatataatc aatttggcga tgttacatta ctggacacac
    67081 caacattttt ccaaggtata cgtttgggtg aaacaatcaa tgtacaaatc gaacgcggaa
    67141 aaacgcttat cattcgttta gatgaaatcg gagaacccga tatcgaaggg aatcgcgtac
    67201 tgttcttcaa tttgaatgga caaagacgtg aaatcactat caaagataat tcgatcatca
    67261 gtacggtcca aacccgattg aaagctgaac cgacaaaccg agaacaaatt ggagcaacga
    67321 tgtctggttc tgttttggat gtattagtga aaaaaggcga taacgttaaa aaaggtgata
    67381 cgttgatgat tactgaagcg atgaaaatgg aaacggccat cgaagcacgt tttgacggag
    67441 aagtcgcaca tgtatatgtg tcttcaggag acactatttc gtctggtgac cttttgattg
    67501 aagtgactga gaaataataa gcgaaaggtg gggaaacatg aaaagactgg gtttgttttg
    67561 gctgattctg ctattagtta ttgtggcatt ctacatggaa ccagtcttgt ttccgccaaa
    67621 acttgaagtg aaggaaaact ctgatcaaca aatatcaaac caaatgacaa cgtggaaata
    67681 ccaggagctt aaagcaactg gtttttctac ttatatcgga aaaccgatcg atcagctgga
    67741 aaaggatttt ggccaagcct atgatcgatt aactagcgga tttggttttg aaacaagata
    67801 ttatgaaaat aaagctagtc atctcacatt tgaagcgaac atccaagaca gcagagtcac
    67861 aactgttaaa gtactgcaaa cagggcctgc tgatatcgcg ccgttttatt tagggatgac
    67921 gatgcaagat cttacgaaaa tcactactat ctatactaat ttcacttttg agtaccatga
    67981 tacagaagtt ggaatcgaac taatggaaga ggatatgaat tatcgtccgc taattgcttt
    68041 tgataacgac acttttgcta ttttattttt tgatcaaaca acaaacggcc tattttcagt
    68101 tgtgtattta gataaagata gtcttttgaa actattgcca tatcaagtgt ttggtgaagg
    68161 ccttccacat tatcaagcag ataaatcggc tgattgggaa tcaatcaatc gcgttaaaga
    68221 acgaaaaagt acgactttat taaatatgct tagaagagaa gacgaacttc ccatatacaa
    68281 tcaaacactt gcctttcaaa accacagtca gctgctacta catgattttt tgacaaggcc
    68341 tgaggaaata ttgtcagagg aacgtatggc tgattggcaa aaatcgctag gcagtgctca
    68401 aacgacagtg aaattctcac tgacgaatga tgaattggag aagttaatca gtaaacaaaa
    68461 attagaaaat actagtgggc tatttattca tccggcgatt gacgctactt tttcgttttt
    68521 atattggtac agcgatcctt acaaccatga ccgtttcatg gacccgggaa cggattctgt
    68581 aggcattgct ttttctaaag aaaatatgtt agtcttattg caagaagaaa acggagaaag
    68641 tagtgatagc agtgatcgtt aacgaagagc tgtttcaact ggaggatcaa tgcctccaag
    68701 tcgctgaaat gataaaaaag agtaaaacga tggcttctta tcttgaacat aaacagtcaa
    68761 tggaagaatc tgcaaaagtt tgccagttga aagaagcgtt cttgtctaag aaagaagcat
    68821 ttgaaaatgt agcggcatat ggtgagtatg cacctgatta tcgtgaaaaa aaacgagctg
    68881 ttcgaaaagc aaaaagacag cttgatatgg atgagcatgt tgcagctttc cgatatagtg
    68941 aaacacagct gcaaagtctg ttggatcaaa ttgtccaagt acttgccgaa gaagtttcta
    69001 atgaaatcaa agtagatgca ggtagcccgt tttttgaaac gaaaaaacac tttggatgcg
    69061 gaggtaattg tcatgcaatt agacaatagt gaatttgaaa taacaaaacg acgcgggctg
    69121 atcgtatggg tctacagctt aaaacaatta aagaatctga aaaagttcgg cttgttgcat
    69181 tatgtctcaa gaagaatgaa atatgtcgtg ctttacttaa atgaagagac attggaagaa
    69241 acagaagaac gaatccgtaa attacatttt gttcgaaacg tagaacgttc ttaccgaccg
    69301 gatatcgaaa tgaattttgc cgaaaaaata ggaaacaaaa cagtattaaa agacgaaggt
    69361 ttcgaaattg aagagctaaa tacagaaatc cgattagctg aacatcttta aaaatctctt
    69421 atgagcagtt tttaaataaa aaataacttg gataacgatc gatttttgat cattgtccaa
    69481 gtattttttt ggtatactag taccagtacc atttctaagg agtgagtaga tgcggataat
    69541 atcaggaaat tatggcgggc gccgtttaaa aagtttagca ggggcgaaca ccagaccaac
    69601 tacagataaa gtaaaagaat ccatattcaa tatgattgga ccatattttg atggagagac
    69661 agtattggat ctgtttgcag gaagcggtgg tttagctatc gaagcagtat ctagaggctg
    69721 cagtcatgcg gtatgcgtag acaaaaatta ccaagcgcta aaaatcatca aagaaaacat
    69781 cgaaattacg aaagaaccag agaaatttac cacaataaaa cttgatgcgg acaaagcaat
    69841 cgaacggcta gcagcggaga aacaacaatt cgactacttg tttcttgatc cgccatatgc
    69901 aaaacaaaaa ataatcgatc aaatccataa aatggaagaa ctgggacttt tttctaaaac
    69961 cgcagttatc gtatgcgaaa cggataaaac agtcgaattg ccagaaactg tggctggatt
    70021 ccatcaaata agaaaacaga tttatggcat aacggccgta actatctata gaaaagaggg
    70081 aaatcaatga aacgtgcatt attcccagga agttttgatc cttttacaaa aggtcactta
    70141 gatacggtgg aacgtgcggc taaattattc gatgaggtag taattggtgt ttttatcaat
    70201 acttcaaaga aaagtttgtt tcccccagaa gaaagaatga cattgatcac aaaagctgtt
    70261 tctcatcttc ctaatgtaaa agtcatgcat caagaaaatc aattgacggt agaaacagca
    70321 aaagagattg gtgcagatgc gttgatcaga ggcatacgaa gtatcaaaga ctttgaatat
    70381 gaacgagaga ttgctcagat gaatcatcat ctatatgatg aattggaaac tgtcttctta
    70441 ttagcaaaac cagaatatag tcacgtgagt tccagtattt taaaagaagt actgcacttt
    70501 ggcggagatg tgagcagcta cttaccgcca gtgatcaacg aagctttagc tggaaagcgt
    70561 gaatcaaatg aaagctaaac aatggttgaa aacagtactg attatcatat ttggaatgtt
    70621 gatcattgga tttgtagtag ttcccttgcc gtattatgta gaatcaccgg gagccacgat
    70681 cgatctgaaa gaactaatta cggtcaatga taaggaagat aaaggttctg ggtctttctc
    70741 tcttacctct gttggtatca ggcaagctac aggtttctta gctttaaaag ctaaaatgac
    70801 cccttttgaa gaattgatca gtgaagaaga attgactggt ggggcgacaa atgaagaata
    70861 taatcaaatg caacagtatt atatggattc ttcccaaaat gcagcaatcg aacaagcgtt
    70921 aaaacttgca gacaagcctt ataaaatgga attcaaaggc gtatatgtca tgagtgtaga
    70981 gaaaacatcg aatttctttg gcaagctttc agttggagat acagtaacaa aagtagacgg
    71041 gaaaagcttt caaagtacag aagcatttat ggattatgta aaaagccaaa aagttggaca
    71101 aacaatcgaa atcagttatt tgcataatgg tgaagaaaaa acagcttctg gtgatctgat
    71161 cgagttacca acagataaaa aggcaggaat aggtatcacg cttactgacc ataccgagat
    71221 cgaatcagat acagatgttc gtattaattc tggctctatc ggtgggccat cagcgggatt
    71281 gatgttcact ttagaaatct atgaacaagt cactggtaaa aacttgcgac atggtaagca
    71341 aattgcagga actggaacga tcaacagtga aggagaagtt ggccgaatcg gaggaatcga
    71401 taaaaaagtg gcaagtgcgg ataaagctgg cgtagagatt ttctttgcac cagatgatac
    71461 gatttcagac gatgttaaga aagaatatcc tgaaataaaa accaactacg aagaagcaaa
    71521 agctgccgct aaaaaattga attcctcaat gaaaatcgta ccagtaaaaa cagtacaaga
    71581 agcgcttgac tatctagaga aaatgaaatg aaactaaata attaaagagg ggttgtgaca
    71641 cgagttctgt cacaacccct ctttttcgtc aaaaatcttc acgataaaaa taaaaacaaa
    71701 agaaaaaatt gacgtaatta gatgttgaag ggacgatttt gccagagctc actgtttctt
    71761 caattttaaa tgaaaggagt aatgaatgat ttttactggt cattctttga aaaaagaaca
    71821 atgatagaaa aaatcagcaa gaaaatacta gcttcaccca aattgatcat ttggatgtct
    71881 atgatagttc ttatcttttg tatcgcaagt ggttttttat tatcaaattt gctattttat
    71941 agagaaacag aagagactgc tgcaaaagaa atactttctt cgtttagttc cagtggagaa
    72001 gatccatcag ctgaaggaaa cgatgtagaa gaggatgctc gtccaaaggt catgtacacc
    72061 gatatcaaag gaagtgtgaa ggagcctggt atctattcct tttcttcaga agaacgggtc
    72121 tatgatgtac taaagcgtgc aggtggatta ttagaagaag cggatagcga tcgaatcaac
    72181 ttttctgcga agatagaaga tcagcaagtc ctatatatcc ctgctgtagg tgaagagctg
    72241 ccagaacatc ttgatcagtt ggcatcctcg gaaggaaaac agcctacagc agattccgag
    72301 cctcccaaaa taaatatcaa tacagccact ccatcagaac ttcagcagat tcctgggatc
    72361 ggatcagtca aagcgcaaga aatcattcga tttagagaag aaaacggatc cttccagaaa
    72421 gtggaagacc tgcaggagat ttctggtatc ggtgaaaaga cagttgaaaa actaaaaaac
    72481 ttcgttacaa ttaaatagaa tgattcaaaa tcgactggca cgagcaataa atggacgcta
    72541 atggatacta agaataatcc aattctaaga gcttggcagg acgaggaggt agtatttttg
    72601 gataggaaat agcttgattg ttcgcaaaga aacattaaaa aaaccaatgc tgaatgtcta
    72661 tctccaaaat aaaatatctg ggattcatat tatgaatacg gctgtcagcg gcaataacag
    72721 tcaagctcta cgagaacgct ttgccaaaga tgtacttagc tatacagcag ataaagtatt
    72781 tattctcatt gggacgaatg atctagcaga gcataaacaa ctttctaaag aaacgtacca
    72841 aaaaatctgc agtggatgat agttcagctg aaaaaagact atagttctga tcatgctttc
    72901 tttatcacgc caccagcagt cgatgagaca aaacaaaaat atcggaataa caagttgatc
    72961 aaagaatata tctctgttct cacagaagta gcaaaacgcc atgattgtga agtatcgatt
    73021 tttatacgga atttcttgaa caaatggatt cgaagaattt ttataatggg ctaatggatg
    73081 acggacttca tttcggcagt gaagggtagg atcttttgtc agacaggatg attcgttata
    73141 tttaaagaat attttgttta agcaatgagt actttttaat caacttgaaa aaacggagga
    73201 atataaaaca tgttagaaaa atcttcaaaa gcatgcgata cgaataaaga aaaagttact
    73261 gataagtcac ataatgaacg cattccttgg gaccagtact ttatggctca ggcagtactg
    73321 ttatctttac gtagtacgtg tacacgttta gaagtaggag cgacactcgt tcgagaaaaa
    73381 aggatcattg ctggcggcta taatggagca gtcagtggag atgtccactg catagatgaa
    73441 gggtgttacg tcgtggatgg acactgcttg cgaacgatcc atgcagagat gaatgcactc
    73501 ctacagtgtg caaaacttgg tatcccgacg gaaggttcag aaatctatgt cacacatttt
    73561 ccatgtttgg cctgtacaaa ggctttgttg caagctggtg tgaaagggat caattatctc
    73621 catgactatc gaaatgatcc ttatgcccaa gcgttgatcg aacaattagg tgtatctgta
    73681 caccaagttg ctttagactc acagcatttt tcaaaacttc aaaatgaatt aacaggaaaa
    73741 catgtctagc gttttagaaa ggcatgttcg aaatcggttg atttttccgg caattacgtc
    73801 gatgattatc tgtttgttgt attacactga caattggggt tatcgtttgc ttcttgccgg
    73861 aattttttta tggttttcgc taaaagaagg acgttttatc gcgtacttgt gtattttgtg
    73921 tagcataacg gtatccattt cttataggct acaagagtat tcatttttag aggaaaaaac
    73981 aacatctgcg caaattactg tatttacaga tacgattcgt gcaaatggta attttttgac
    74041 aatggaagga aggttatcag agaataaaca aaagctccag ctttcttatc aagtaaacac
    74101 agaagcagaa ttgaaaacat ggcttaactt ccgaggaaag ggaatagaat tgactgctga
    74161 aggcgagttc atctctccag caaaaaagcg gaatctttat aattttgacc aagaagctta
    74221 cttcaggaac catagaattt ctggacggtt ccaaattcaa aagattgaac agatcaccat
    74281 gcaaaatcac tggcgaaact ggcttcagag aaaaagagga tcttttatct cctttgtcca
    74341 atctgaattt ccgcaaaaaa caagtgtata tattaattct ttattatggg gctataaaga
    74401 tgcaggattc agagaaccag aagaaatttt tagggagagt ggtttgctgc atctgttcag
    74461 tctatcgggt ctacacatcc aattttattt agggtggctg tattatttat ttcgacgtat
    74521 cggatggcca ctacacgtca gtattattcc gttgagtcta ctaactatct gttatgtttc
    74581 tattgcagga agttctattt ctgttttgcg tgctagtttg ctgtttttat tgcgattgct
    74641 agtcaaattg ctgaataagc attattccac catggactta tttgcagttg tcttttggct
    74701 tcttttacta tttgatcctc gatttctttt tcatgcgggt ggacagctta gttttttcat
    74761 gagttttttg tttatcctga tttcttttga tggagattgg ttgaaaaaaa cggcctctca
    74821 cgtcctgttt actatgatca cgatgccgtt gattgtatgg catttttatg agtggcctat
    74881 tttaggtagt ctttttacat tactgattgc accggtattc aagtttcttt ttcttcctgg
    74941 tgtgttattt ctcacactct tcaatcgctg gctgccggaa actttacttt tgctgataga
    75001 agagggatta atcttatttg agcgaataat cgatttttct tcagggtcgt ctctcattat
    75061 aggacgtttg ccccttgtgc tatctgggat gtatattttc ggattactga tttgtatgga
    75121 taggttgaaa gaacaacctg tgaaacactt gttttatgtt ggtctttttt cttttatttt
    75181 tttgactttg ccatttttac gcttttcttc aacgatcgct tttatcgatg tcggtcaagg
    75241 agacagtatc tttttgcaaa gtccttttcg aaaagaaacg attttgatcg atactggagg
    75301 tcagttacac tttgaaagag aaaagtgggc gcaaggtatg atacgtcctc ctgctgaaag
    75361 aaatatcgtt ccatatctaa aaggtcaggg gataagtgta atcgataaac tcatcttgac
    75421 acacgacgat acggatcatg tgggagaatt acccacgtta tctcagcatt ttactatcaa
    75481 aaaaatttat atcggttggg gcgctggacg aaatgagcca ttacaaaggc atttatctga
    75541 agctcaaaaa aatggtactg agatcattga aatcaaacat ggggacagga tcagcggtta
    75601 ttatgatctt tatgtattga ctccttttga aaagggagag ggaagaaatg aagattcgat
    75661 tggtctatgg atggaatata acaataggcg ctttttattt ttaggagatt tgaatcaagc
    75721 aatggaaaaa caattgcttt tgatttatcc taatctgaaa gccgatgtag tcaaattagg
    75781 tcatcacggt agccgtacgt caagtaacac agattttctt tcccatattg aagcaaaaca
    75841 tgggattata tcctgcggcg taaataatcg ttatggtcac ccgcacagcg aagtattgga
    75901 gacactagaa gaaaatcaga taaaaacatg gcgaacggat caacaaggaa tgatcaccta
    75961 tcgctggcat ccagtttttc atcctcatgg gctagtggaa acaatgatag actagtaatt
    76021 ctcatctgct catggtagga taatttttga aagtggtgag taatttgagt atacaagcag
    76081 agttacaaaa aatacgtaaa gaaaaacttg catcttgtta cttgatttta ggaacagaaa
    76141 agtttctcca agatcaagtt cggacagaaa ttgtcaaaaa aatccaatta gagggcgaag
    76201 atgacttaaa ttttttaagt tttgacatgg aaaatgcaag tttagatgag gtagtagctg
    76261 aagcagagac gttgccgttt ttcggagatc aacggttagt attcgtagag aatccttatt
    76321 ttttaacagg tgaaaaagta aacaatggaa tcgaacaaaa tactgattta ttgacggctt
    76381 atttgaaaga accattagaa tcgactgtat tagtattctt tgcgccatat gaaaagttgg
    76441 atgaacggaa aaaagtaacg aaacaattga aaaaaacagc aattatcatc aatgtccaac
    76501 agttgaatga aaaagaagta cgtcaatatc tgatgaatac cttagagaat tcctcaatca
    76561 aaatggaacg aagtgcaatt gatttattct tgaggttgac cgacttagat ttatccaaaa
    76621 tgatgagaga gttacagaaa ttggtccttt atggacaaaa tcaacagcaa atcacggtaa
    76681 gagaagtaga agaattagtt cctaaaacgt tagaacataa tatttttgac atgacccaat
    76741 atattttaaa agggaagacg gaacaagctt tgcgtttata cgaagattta gttatgcaag
    76801 gggaagaaac aatcaaaatt aatgcgattt tgctttctca attacgttta tttttacaga
    76861 caaaatttct tgtaaagatt ggttatcagc aggcaaatat tgctgaaaca ttgaaaatcc
    76921 atccatatcg tgtaaaactt gccatgcagg aagtaaaaaa attcgatgaa catttgctgg
    76981 tacagttgtt tgatcaatta gttgagatgg attatcaaat caaaacggga caaatcgaaa
    77041 aagaattaag ttttcaacta ttcgttttgc gcacagggca gttgttaaga aatcgataag
    77101 aagaaagacg agggcaaagc atgcgggaag gtcgtttgtt tttgtccttt ttttgacaaa
    77161 taaaaaaatc ggccgaagcc gattctttta tttagcgatt tttttgctta aacgagattt
    77221 gtcgcggtta gctttgtttt tgtggattag accttttgat tcagccatat caatagcttt
    77281 aacagcttct ttatataacg catctacgtt gtcagcacct gaagctacag catcttcaaa
    77341 ttttttgatt gcagtacgca tagcgcttgt ttgagatgaa tttttcacat tagcgttttc
    77401 gcttgtacga acacgtttga ttgcagattc aatgtttggc attagtttca cctccgataa
    77461 cttaagttct tacgcgtgtt accgcataat tcaacattca ttattatacc taaaggcagc
    77521 gtgctttgca atatgaacat gacaagtttt tttccttaac agtcttttat taggattttt
    77581 tttcagtaaa aacggatagt acaagctgaa aaaaatcaaa aagacgctat tgtgggaggc
    77641 aatagcgtca aaccaaacta gatttttatt tatctattta aacaaagttt gaatgggaat
    77701 gtttggtact aagttattat ataacaaaga gaaccgtttg caaagttttt tttgaaaagg
    77761 gatacgattc cctgttgttc tttgtttttc tattaagaaa ctgcagttat tttataacca
    77821 gtatcctttg atcagtaaag gctattctgg tgtaccgatt attttaatgt attcatcgat
    77881 attttttatc aacatttttt ttatgaccaa ctggttccga cggttccatt tgccacttac
    77941 ttccagcaca tatttattta ggggcaaacg taagatatat tttgccaaat agcgatcttt
    78001 aagcaggcca ttgatcacac ggtcctcaag ctgcaagttg atcccagtca gcatcattgg
    78061 ctgttcttgt aaaacgtacg tatcaattaa aagaccttca aaagtttcca caagcgtctt
    78121 cctttctttt caaagtaacc tgaaaacagg cactaattct tccattttga tattcgttga
    78181 agtggatgcg atcattcttt cttttgtcca ctgtaatcta gtataacaaa aaagagcaga
    78241 agagccaaaa agaaaaaaga gcatcgaatt ctataggaag tctcattggc acggcctaaa
    78301 ttattacaaa acattgatta aaaaaagccc ctgcatcgca ggagcatccc ctatatcgtt
    78361 ttaataggtc aatcaaaaag cagtatcaaa cacattttgt atcaaatcag gaaataatag
    78421 ataagctaag cccagaatca aaagtaccgc tagaagaaac cagccgattg ttcctatgaa
    78481 atactttaat ttttcgaaca ttgtatcacc tcgttgtcag tatatcacgg atcagtaaat
    78541 tgggggtgtt aagaatctcc taaaatagac aaaatttgtt ctattttcct aaagcaaata
    78601 cttgtaagat acagtaagtg ataccagaag caatgaccgg tccggcggcg attcctctta
    78661 agaagacaac acctaaaatc gtgccgaaaa caagtgcaac agtgatttgt ggatcagaag
    78721 caatcaatcc tacacccttg gaagataaga cagccactag cccaccgcaa aaaacagcta
    78781 cccagccaat aggggattta aaggcttcta atagatcacg tagaccgatt tctcctgttg
    78841 cgatagggat tagaatagcc gctgtgatga tcgtcacccc aatattgatt cctttgcctt
    78901 gaagccaagg gaaaaaggaa cgggaggctg gtatgagctt cataatcaaa acaaaagctg
    78961 ccgctatcat cagactttga tttttagcta aaattgctac aagtaaaata attcctaaaa
    79021 aaagccaact ttccatgaaa tatattcctc actttcttcg aaacaatcat aacatgtttt
    79081 tagaaagtta caaaagtagg aattcatcct tcaagaccta ttcataagct gtttttttca
    79141 taaaataaac aaaatcataa aaaaacactt tgcttatttg acagaagaca gccgcgcttt
    79201 gtatagtgga caagacttac atttttttga agaatcagaa agatcaggta aaggtcttag
    79261 taaacgcaag ccattcagaa taaccaatat tgtgcttcct tcgtgtccta caactccaag
    79321 aggtagattt aaaaattgca ggaagttaga agcaatcaac aagagaataa caagaatcga
    79381 aaaaataata ttttgtttga tgatccgccg catttttaaa gatagccgat gactgtagac
    79441 aaatttattt aagtcgcttt tcattaagac gatgtctgcg atatccatag cggcatctgt
    79501 cccttgcccc atagcgacac ctaaagaagc ggctgctaaa gcaggcgcat cattgactcc
    79561 atcaccaacc atcgcattta cttggtaaag ttcattctct tttttgacta gttgtgtttt
    79621 ttcttccgga gtacagcttg catagtaatg ttcaatccct acttgttcag caatggcctt
    79681 tgctgtgcca gaatggtcgc cggttatcat acttgtatgg atgttttggg atttaaagta
    79741 ggaaattact tgttgggtat ttgcttttgg aatatctagc agacctaaaa cagcaattaa
    79801 ctgctgatct ttagaaagat aaatcacggt tttgccttga ttttccaaac gttcgatcat
    79861 ttccgcaatc tctttgctga ttatcatcgt ctcaggatca tatgcgtgct tgccaacttt
    79921 ccattgcgca cctaaatagg tagtttgtaa tccaaaacca gtaatttctt ctattggtaa
    79981 gtattggatt tcttcaggta ttgtgcaatc gaaatattgc aaaatcgcat gagcaagtgg
    80041 atgcgtcgtt ttttgttcca tagcaacaag aagttgttgt gccaaatcct tttcatcaaa
    80101 gaaaaagctg tcagttacaa ctggttttcc tttagtgagc gttcctgttt tatcaaaggc
    80161 aatcgctttt aattcagaaa gcttttctaa atggataccg cctttgatta aaatcccatt
    80221 ttttgcagaa tttgaaagag cagctagaga agctggtgta gcagatgcga caagcgcaca
    80281 aggagaagcc acaactaata ataccattcc tcgataaaag ctttcatccc aactccaacc
    80341 taggaaaaag tgggggagaa ggatcattag aggaacagca agcaaaatca attttacata
    80401 attattttcg attcgttcga taaatgaagc tgtttgagtt ggcgtattct gcgcttcttc
    80461 tactaattgg ataatcttag caaagacagt ttcggaactc gtttttgtta ctcgaatcgt
    80521 gatgggattt ccttgattca gcgttcctgc atacatctca tcgcctaaat atttttctac
    80581 aggcacagat tcaccgctta atgcagactc atcgatcgtt gcctgtgtgc tttctaaaaa
    80641 gccatctatc ggaacagctg ctcctttagc gacaaatacc aagttatcca cctttagttg
    80701 agaaacatct acttcttgca tttgtccatt ttcttttaat aattgggctt tttgtggttg
    80761 aagattcatc aaagcagtaa tttctttttg acttttatta gttgtgtatt cctctaatgc
    80821 accgcttaaa caaaagatga atgtcaacat tgctccttca aagtaattac cgatcaaaca
    80881 ggcaccaata gcagctaaag ccatcaataa atccacattc agtgtccgat cattccacaa
    80941 ttctttcaag ccttccgatg tttgtttata tccaccgatg ataatggcac tgatataaaa
    81001 aagtggggtg atggtaagag agaatcggtc gaagataaaa ccgaagatca taaataaaag
    81061 gcagccgatt gttgctgcaa aagctttttt ctgattagtc atcgttatta gacctccttg
    81121 tcaaaacaag gatagcataa taaaaatgta attgataata ataacagcta aatgagaacg
    81181 ataatggtta tcatttggtg attaagcttg atgttgtttt tattatttat aatgattatt
    81241 attaaagaaa ataaaaaacc agaacatttc tcaaataaga
//
LOCUS       DAYYDS010000005        68785 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000005 DAYYDS010000000
VERSION     DAYYDS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.005"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..1055
                     /locus_tag="ACHA02_000353"
     CDS             174..1055
                     /locus_tag="ACHA02_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8668736.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1068..2678
                     /locus_tag="ACHA02_000354"
     CDS             1068..2678
                     /locus_tag="ACHA02_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8668737.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2755..3414)
                     /locus_tag="ACHA02_000355"
     CDS             complement(2755..3414)
                     /locus_tag="ACHA02_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8668738.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3697..4362)
                     /locus_tag="ACHA02_000356"
     CDS             complement(3697..4362)
                     /locus_tag="ACHA02_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8668739.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4509..5114)
                     /gene="dhaL"
                     /locus_tag="ACHA02_000357"
     CDS             complement(4509..5114)
                     /gene="dhaL"
                     /locus_tag="ACHA02_000357"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8668740.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5142..6152)
                     /gene="dhaK"
                     /locus_tag="ACHA02_000358"
     CDS             complement(5142..6152)
                     /gene="dhaK"
                     /locus_tag="ACHA02_000358"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8668741.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6127..6522)
                     /gene="dhaM"
                     /locus_tag="ACHA02_000359"
     CDS             complement(6127..6522)
                     /gene="dhaM"
                     /locus_tag="ACHA02_000359"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8668742.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6676..6996
                     /locus_tag="ACHA02_000360"
     CDS             6676..6996
                     /locus_tag="ACHA02_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8668743.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7108..7965)
                     /locus_tag="ACHA02_000361"
     CDS             complement(7108..7965)
                     /locus_tag="ACHA02_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8668744.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(8073..9376)
                     /locus_tag="ACHA02_000362"
                     /pseudo
     CDS             complement(8073..9376)
                     /locus_tag="ACHA02_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9373..9882)
                     /locus_tag="ACHA02_000363"
     CDS             complement(9373..9882)
                     /locus_tag="ACHA02_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668745.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(10057..12192)
                     /locus_tag="ACHA02_000364"
     CDS             complement(10057..12192)
                     /locus_tag="ACHA02_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8668746.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12275..13930)
                     /locus_tag="ACHA02_000365"
     CDS             complement(12275..13930)
                     /locus_tag="ACHA02_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8668747.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13932..14246)
                     /locus_tag="ACHA02_000366"
     CDS             complement(13932..14246)
                     /locus_tag="ACHA02_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8668748.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14416..14676
                     /locus_tag="ACHA02_000367"
     CDS             14416..14676
                     /locus_tag="ACHA02_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8668749.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14690..15004
                     /locus_tag="ACHA02_000368"
     CDS             14690..15004
                     /locus_tag="ACHA02_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8668750.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15734..16288)
                     /locus_tag="ACHA02_000369"
     CDS             complement(15734..16288)
                     /locus_tag="ACHA02_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8668751.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16414..18342)
                     /locus_tag="ACHA02_000370"
     CDS             complement(16414..18342)
                     /locus_tag="ACHA02_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8668752.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18648..19364)
                     /locus_tag="ACHA02_000371"
     CDS             complement(18648..19364)
                     /locus_tag="ACHA02_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8668753.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19365..21188)
                     /gene="glpO"
                     /locus_tag="ACHA02_000372"
     CDS             complement(19365..21188)
                     /gene="glpO"
                     /locus_tag="ACHA02_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8668754.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21202..22698)
                     /gene="glpK"
                     /locus_tag="ACHA02_000373"
     CDS             complement(21202..22698)
                     /gene="glpK"
                     /locus_tag="ACHA02_000373"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8668755.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22834..24294)
                     /locus_tag="ACHA02_000374"
     CDS             complement(22834..24294)
                     /locus_tag="ACHA02_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8668756.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24284..25579)
                     /locus_tag="ACHA02_000375"
     CDS             complement(24284..25579)
                     /locus_tag="ACHA02_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8668757.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25911..26588)
                     /locus_tag="ACHA02_000376"
     CDS             complement(25911..26588)
                     /locus_tag="ACHA02_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8668758.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26618..27709)
                     /locus_tag="ACHA02_000377"
     CDS             complement(26618..27709)
                     /locus_tag="ACHA02_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8668759.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27810..28244)
                     /locus_tag="ACHA02_000378"
     CDS             complement(27810..28244)
                     /locus_tag="ACHA02_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8668760.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28828..30285)
                     /locus_tag="ACHA02_000379"
     CDS             complement(28828..30285)
                     /locus_tag="ACHA02_000379"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8668761.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30282..32177)
                     /locus_tag="ACHA02_000380"
     CDS             complement(30282..32177)
                     /locus_tag="ACHA02_000380"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8668762.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32235..34010)
                     /locus_tag="ACHA02_000381"
     CDS             complement(32235..34010)
                     /locus_tag="ACHA02_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8668763.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(34060..35556)
                     /locus_tag="ACHA02_000382"
     CDS             complement(34060..35556)
                     /locus_tag="ACHA02_000382"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8668764.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35750..37372)
                     /locus_tag="ACHA02_000383"
     CDS             complement(35750..37372)
                     /locus_tag="ACHA02_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8668765.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37821..39134)
                     /locus_tag="ACHA02_000384"
     CDS             complement(37821..39134)
                     /locus_tag="ACHA02_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8668766.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39379..39603)
                     /locus_tag="ACHA02_000385"
     CDS             complement(39379..39603)
                     /locus_tag="ACHA02_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668767.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39623..40045)
                     /locus_tag="ACHA02_000386"
     CDS             complement(39623..40045)
                     /locus_tag="ACHA02_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8668768.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40189..40899)
                     /locus_tag="ACHA02_000387"
     CDS             complement(40189..40899)
                     /locus_tag="ACHA02_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8668769.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40917..41414)
                     /locus_tag="ACHA02_000388"
     CDS             complement(40917..41414)
                     /locus_tag="ACHA02_000388"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8668770.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41451..41903)
                     /locus_tag="ACHA02_000389"
     CDS             complement(41451..41903)
                     /locus_tag="ACHA02_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8668771.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(42038..42643)
                     /locus_tag="ACHA02_000390"
     CDS             complement(42038..42643)
                     /locus_tag="ACHA02_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8668772.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(43093..43713)
                     /locus_tag="ACHA02_000391"
     CDS             complement(43093..43713)
                     /locus_tag="ACHA02_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8668773.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43715..44584)
                     /locus_tag="ACHA02_000392"
     CDS             complement(43715..44584)
                     /locus_tag="ACHA02_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8668774.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44607..45014)
                     /locus_tag="ACHA02_000393"
     CDS             complement(44607..45014)
                     /locus_tag="ACHA02_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8668775.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(45033..46322)
                     /locus_tag="ACHA02_000394"
     CDS             complement(45033..46322)
                     /locus_tag="ACHA02_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8668776.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46349..46651)
                     /locus_tag="ACHA02_000395"
     CDS             complement(46349..46651)
                     /locus_tag="ACHA02_000395"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8668777.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46676..48769)
                     /locus_tag="ACHA02_000396"
     CDS             complement(46676..48769)
                     /locus_tag="ACHA02_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8668778.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49258..50022)
                     /locus_tag="ACHA02_000397"
     CDS             complement(49258..50022)
                     /locus_tag="ACHA02_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8668779.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(50133..51002)
                     /gene="mdcB"
                     /locus_tag="ACHA02_000398"
     CDS             complement(50133..51002)
                     /gene="mdcB"
                     /locus_tag="ACHA02_000398"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8668780.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(51027..52103)
                     /locus_tag="ACHA02_000399"
     CDS             complement(51027..52103)
                     /locus_tag="ACHA02_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8668781.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(52127..52720)
                     /locus_tag="ACHA02_000400"
     CDS             complement(52127..52720)
                     /locus_tag="ACHA02_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8668782.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52724..54400)
                     /gene="mdcD"
                     /locus_tag="ACHA02_000401"
     CDS             complement(52724..54400)
                     /gene="mdcD"
                     /locus_tag="ACHA02_000401"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8668783.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54390..54689)
                     /locus_tag="ACHA02_000402"
     CDS             complement(54390..54689)
                     /locus_tag="ACHA02_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8668784.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54791..56437)
                     /gene="mdcA"
                     /locus_tag="ACHA02_000403"
     CDS             complement(54791..56437)
                     /gene="mdcA"
                     /locus_tag="ACHA02_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFM8668785.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56444..57355)
                     /locus_tag="ACHA02_000404"
     CDS             complement(56444..57355)
                     /locus_tag="ACHA02_000404"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8668786.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57475..58332
                     /locus_tag="ACHA02_000405"
     CDS             57475..58332
                     /locus_tag="ACHA02_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8668787.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58519..58881)
                     /locus_tag="ACHA02_000406"
     CDS             complement(58519..58881)
                     /locus_tag="ACHA02_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8668788.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(59070..59252)
                     /locus_tag="ACHA02_000407"
     CDS             complement(59070..59252)
                     /locus_tag="ACHA02_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668789.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            59227..60666
                     /locus_tag="ACHA02_000408"
     CDS             59227..60666
                     /locus_tag="ACHA02_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8668790.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60673..63225)
                     /locus_tag="ACHA02_000409"
     CDS             complement(60673..63225)
                     /locus_tag="ACHA02_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8668791.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63339..64670)
                     /locus_tag="ACHA02_000410"
     CDS             complement(63339..64670)
                     /locus_tag="ACHA02_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8668792.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64636..66114)
                     /locus_tag="ACHA02_000411"
     CDS             complement(64636..66114)
                     /locus_tag="ACHA02_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8668793.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66419..68254)
                     /gene="tdc"
                     /locus_tag="ACHA02_000412"
     CDS             complement(66419..68254)
                     /gene="tdc"
                     /locus_tag="ACHA02_000412"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8668794.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19610 a        14630 c        11749 g        22796 t
ORIGIN      
        1 gagaaagtat caagctagca attccttgtt tatgaattca ttgacagaaa tttataaaaa
       61 gaatattatg tactaaaata ataaaaaaag ttttataaaa aggtataaaa cctttttatt
      121 aaactattta atcttgttta gtatatattt ttagaaaaag gagtggggtt tttatggcag
      181 aaatcgtcaa agtcacacat ttgaaaaaga gctttggtcg tttcgaagct ttaaaagatg
      241 tctctttcac cgtacattca ggtgaagtcg ttggatttat cggaccaaat ggttctggaa
      301 aatccacgac gattcggaca cttttaggga ttataaaaaa agacggcgga acggcaacga
      361 tttttgggca agatgtatgg gaagatagtt tatctatcca tgaacgtctt tcttatgttc
      421 caggcgatat ctctttgtgg ggaaatttga ctggaggagc gatcatcgat ctcttcatga
      481 agcttcatgg aaaagggagt gctcaaaaaa aagatgaact gattcaacgt ttctctcttg
      541 atcctaagaa aaaagcaaaa aactattcaa aaggaaatcg ccaaaaagtg ggcttgattg
      601 ctgctttggc tgttgattcg gatctctatt tatttgatga gcctacttct ggtttagatc
      661 ctttgatgga aacgattttc caagaagaag ttgaaaaatt gaaagaacag ggcaaatcca
      721 tcctactttc ttctcatatt ctcagtgaag tcgagcgact agcggataaa gtcgtgatca
      781 tacgcgaagg aacagtcgta gaagaaggaa cactcgatga attgcgtcat ttgactcgtt
      841 cgaccatcac tatagaaaca gaagcagaga atagtcactt agcacaattg aacggtgtcc
      901 atgactttgt gcaaaaaggt cacctcgctc aatttgctgc tgataacgaa gctttcgatg
      961 tgattttagc tgaagcgtcc aaattaggtg tgaaaaaatt tgaatctatt ccaccgactc
     1021 ttgaggattt attcatgaga cattatgaag gatgaggagg aagcgaaatg actgataaat
     1081 tttcacgctg gtccaccctt ttttctcaaa accttagccg tgactggaaa aagatcctga
     1141 tatggctagt cggtgtcact gctttttctg gtggttttgt ccctgcgttt gaagaattga
     1201 cttccggaca aggattagct ggattatacg aaaccttgca aaacccggca atgattgcaa
     1261 tggtcggacc aactccagct tcgaatgctg ctgagtatac tttaggagcg atgtatgcaa
     1321 atgaaatgtt gcttttttgc agtttgtttg ctgctgtgat ctctgtttta cacgtggtga
     1381 gccaaacgag aaaaaaagaa gaacttggtc tgattgaact tattcgttcc tttcgagtcg
     1441 gacgtcaagc aaactcttta gctgtcatcg ctgaaagtat tctgatcaat ttgcttttag
     1501 caatatcgat cagctgtacg atgatttttt tccaagcaga ttcgatcact gcagaaggtg
     1561 cttggctttt tgggctatct gtaggtagta tggggatttt ggggagtgcc gttgcactgt
     1621 ttgctgcaca actgatgcca agttcagcca gttcgaatgg tctgtcttta gcgtttttag
     1681 gtggacttta ccttctacga gcaataacag atgtagctga ttcctccctt tcgatgtgga
     1741 atcctttagg ctggtcttat ttgacctatc catttacaac taacaactgg attcctttgc
     1801 tttatttgtt tttgttcagt ctaatcttga taatcagttc atttgtttta gaggaacatc
     1861 gagatttgaa caccggctat atccccgaac gtgaaggaaa accgtatgct aaacgttctc
     1921 ttttatcctt gcgtggttta ttattctatc taaataaagg gatgacaatc agttggctta
     1981 tcacttttgg tttgatgggg gcggcatacg gctctatcta cgggaatatg caacagtttt
     2041 tagaaagtaa cgacttaatg aaacagatgt ttatccaatc tggcgtctca attgaaaaaa
     2101 actttacaag cacgatcatg gtcgtcttga tttgtctagc tgctatttta ccaattgcta
     2161 gcatcaatcg tttatttaca gaagaaaccc acggacgtat gaatcagctg ttcgcaacaa
     2221 aaatttctag gaaccaactt tattggacca atatgttact agctattcta tttgctatat
     2281 tggcaatcct gattagttct ggtgcactag ggctttctgc tttggcttcg atgaaggatg
     2341 cgaaagacat ggatctattg atgtttctta aagccgggtt caatttcctg cctgccgttt
     2401 tagtgtttat cggaattagt ggctttctat tcggatggct tcctcgaatg ggtaaacttg
     2461 cttatatcta cttagcttac tcctttgcac tcaattactt tggcggaatt ttggatttac
     2521 cagaatggtt ttccaaaaca gctgtcccta gctgggttcc acggatgcca aacgattctt
     2581 ttgatctttc cgtgttcctt acgttatcat gtatcagcct tgcactgttc gtccttggtt
     2641 ctattggtta taggcgaaga gacttactag aaggataata aaaaagcatg tacaatttca
     2701 tgctagcctc caaatctggg tcaaaaatcc aacatttgga ggtcattttt ttatttacaa
     2761 gtcttctaac tgctctttat caatgatcca gacttctcga ttctttcttt ttacataacc
     2821 attctcctct aataatttaa attttctaga taaagtttcg ggggtcgtac cgataaatgc
     2881 agcaagttct ttcattttca ttggcaggaa aaagtgatcc ttccctgtcg ctttagctaa
     2941 gtcaagcaga tagacagcga ctcgttcttc tactttttcc atcatcaaaa aagttgtctg
     3001 ctgctgtact tttgttaatt tctcaacgtt gattgctaaa agtttcagac taagggcggg
     3061 ataatgcagt aagagttccg agaaatcttt ttgtctcaat atgcaaattt ccgttttttc
     3121 tagtgcttcg ccaaataaag actcatttgg aacgcctagc aactggctct ctccctcata
     3181 tccgcccggc tctattactc gtaaaagctg ttcctttccg ctagcagata attgatacac
     3241 tttcatactg ccggaggcca cgatcactaa ttgaggttct ccattcggcg caagaatctg
     3301 ttctcctttt tccacatagt gatgtttgac aagtgcgtgt actttttctt gatcttgctg
     3361 tactaagtga ttgaacaaag gaacaaatga aacacataaa tgatcatgac tcataatctc
     3421 tcctccttct aaacagctat ttctaataaa gaaacataac ttcccttatt ccaactgata
     3481 attttctttt atcatagcat aaacgtcaag cgaaccagat accagcaaat agagtggatt
     3541 tctttttgtc ccttgcttta ttttccttct tttgagacct taagtaattg attcctatca
     3601 attttttaaa tatttacttt ttgaataatc aaaaacaaac cttcactcgc aagccgagag
     3661 taaggtttgt ttttcgcatt gcttatttcg tgttgtctat tgaaattttt ccgcacctag
     3721 cttttgatat agtggtgcta gttcttgtaa ggtttcagcg gttatttgct caacgtgttc
     3781 gatttcttgc ggatagaaga atagtttacc catttgaaag gttcggtctc tcatcgcttt
     3841 tttcaactct tcttgtttct ttgtatcgga caaatagtca gacaaagcta agtggtcgtc
     3901 aagttcatgt tctggttgcg gccatatgta ataatccttg atatcgactg ttttgctcca
     3961 ctctttcagt atcgcaagca actggttata cgctgcaatg ctaccatatc gttcttcgct
     4021 tttataatgt tggtacatca aatagatttg gtattgtttt tgattcaaca aaactgctag
     4081 acaggcattc tcgtcttgac gagattcgct tcggtacgcg ctccaaaaat gactgcgtaa
     4141 attccagcca ttcgtccagc tttcgatttt aggtttgctc atgccgtaca tatcaggcaa
     4201 aagtgcagct gtctggagct gaatctcttt ccatttttgc cagtgttctt tatattcttt
     4261 cttgatttgt tcaatcttat ctgttggatg gctctctttg agctgtttga atgcgtagat
     4321 tggtcgatca aacaactgat aatctgattt tccaagttcc attgtttcgc ctctttccgt
     4381 tatcttttgt attctacctg aattttgcga tatggacaaa tagaggttgt gaaaaagctg
     4441 ttctgcatca agtcataaga acagccaaac aaaaaactgc gatgagcatc accgcagttt
     4501 tattgaactt aaggttgatt gatcacatct aaaagcgttg aaaataaaat ggcactagaa
     4561 gcagctcctg ggtcgatatg tccgattgca cgctctccta gataggaagc tcgtcctttt
     4621 ttagctacca agactttggt gtgttctttt gcctggtcaa tgatttcttt tgttaaattc
     4681 cctgccttca atgcctctgt gactggcagc caaacatcta acatcgtttt atcgtcagct
     4741 tctgcctttc ctcggctttg aatacccgct gttccctgct cgatcacttc gcctaatttt
     4801 tcttgagaat caatagaatc gacctctttt gtcgctttca tcatgttcat gaatgcagat
     4861 ccgtataacg gaccagaagc cccaccgact ttcgatatca tcgccataga gaatactttg
     4921 aatgtatcgc tgattgtttc aggatgatcg gcttgaaaag cttcttcata agctgaaacc
     4981 cctcttccca tgttatttcc atggtctcca tcgccaatcg gggtatctag atcactgaga
     5041 tattgtttgt tctgattgat tgcctctgca aattgcgtga gccattcttg gatttcttga
     5101 acggttagtt tcatattatc acccttttat cttgagattt tttaccatga aatcgtctgt
     5161 acattgcttt ctaaagaatc tttccattgt gtctccgtca agtcgatcag tgttagagaa
     5221 agaccttgca tatctagtga ggtcataaaa ttaccgactt ttcggaaaac tacattgacg
     5281 cctctttctt ccaacagagc taaaacatca ttcatgaaca cgaactgttc catcaaaggc
     5341 gtcccgccca ttccgttgac tagtacacct gcttcttttg gtaaattcga gtaatcatct
     5401 aaaatcttag taacaagttc ctttgccagt actttagaag gctgcatctt ttctcgacga
     5461 tagcctggct caccgtggat accaacacca tactcgatct catcttctcc tagttcgaat
     5521 cctggtttcc ctacttctgg tacagttgct gctcgaagcg ctagaccgat cgtcttagtt
     5581 gcctgtacga ccttttctcc cagttctttc aactcagcta gggaagcacc gtttcttgct
     5641 gcatcgccca cgattttgtg tacaaggaca gtacctgcaa ctccgcgttt tccagcagta
     5701 tacgtactat tttcaacggc gatatcatca tctacgatca cactttctac ttcaatatcg
     5761 tccatagcag ctaattcctt tgccatatcg aagttcaatg catcccctgt ataattctta
     5821 acgatcagca aaactccttt tccactatcc gcttccttaa tagccgtctg aatctgatct
     5881 ggagttggag aagtaaacac atcaccaagc accgctgcac ttaacatccc ttcccctaca
     5941 aacccggcat gggatggttc atgtccgctt cctccaccag aaaccaaacc aacttgttcc
     6001 gctttctgat tcttggctac aacacgggaa gattctacac gatgaacaag ttctggatag
     6061 ctttttacta atccttccag catttcttcg acaatattgc ttggatcatt catgattttt
     6121 ttcaatttat tcccatcctt tctttccggc attttgtgct tctgttaaga tttgctgagt
     6181 atcatctgtt ataccggcag tgatggctgc tgcaaaagct ccttccacta aaggcgcatc
     6241 caccagatga taacgttgct gctgctcttc ttccaacatg tcttttgcca gttccgagtt
     6301 caataccgcg ctaccgatat ccgcaaaaac aaaataacta gctgcatcgc ttgattcatt
     6361 gactgcttct acgattttca tcgggtctga tccgatctcg ccatttattc cgccgagaga
     6421 aaaaatttgc acctcttcgg atttttgcat ctgctcaatc atttctttaa taccatctgt
     6481 tactttttga ctatgagaaa taagcaaaat actttttttc acaagaatct tcctttcttt
     6541 tacatagttg ctaacgattg gtttcgcttt cattaatgaa caattcaagt atataataaa
     6601 tttctaattt tgggaagata tgtgtagtgc ttcttgaagt ttgttatact ttaattaaaa
     6661 gagaggagtg atcgaatgat tacaacaaca acgaacagtg tagagggaaa agccgtagta
     6721 gcttataaag ggatcgtttt tggtgaggtc attactggga tccatgcatt taaagacttg
     6781 gaggcggggt tgcgaaatat ctttggcggc cgctcaaaaa gctatgaaaa cgaactaatg
     6841 ggtgcaagag aagaagcact tgccgaaatg gccgaacgtg caaaagcatt aggagctgat
     6901 gcgatcattg gcgtaaaaat ggactatgaa gtattaggca gcgacaatgg catgctgatg
     6961 gtcacatgta gtggaacagc tgtctccacg agataagtag gggacatctg ttttaataac
     7021 taaagagaga tgggacatgt agtggcactc aagaattaga tttttcagtc taacttcgag
     7081 gtactctacc ttcccatcct ctctttttta tttctttttg cggatgattc cgtaagctgc
     7141 tgcgactaac acacccgccc ctgctaagga ataactgaca gaagctgttt tttcatttgt
     7201 ttttggcaaa gtctgacttg ttgatttgga tggctctgtc gtttgctgaa ctgtttctgt
     7261 cgtgcttcca gaggtcactg atttttctcc tgccgaatgc aaaatatctt ttattcgttg
     7321 gaaactagct gctaagtcag ctttttttgc ttcaaaatca ttagcaactg tttgtgcttc
     7381 gctgatcttt ccgtggattg cttgactggc cgctgtgata tccgcttgag tttgacttag
     7441 ctttccagaa gctgtttgag caacctgatt taattgctga ttgaattctt ttgcttgttg
     7501 gatcttcccg tcaagatctg tttggacagc ttccgctttt gctttcgctt cggcaagctt
     7561 tttctcaagt tgtgcacggt catttcccac tttattttta agatcagcta aatcttgatc
     7621 gattgctgct tggtcttctt tcaatttgtt ttttgcagct tctaatgctt cttcgagttc
     7681 ttttctttta ttttccaagt cttgcaaatg atcgatttgg tcagaagagg tagaactatt
     7741 tattgatgta tcttctgaag atgaaatcgt ttctcctgaa ttatctgtcg gtacattgga
     7801 agtcatttct gtttccgagg caccgttttc agcaccagaa gagcttatag ttgttgaagg
     7861 ttctgtttga gcaggttcag aagtttccgc aaaagctgtc tgtcccaata agaaaatact
     7921 ggaaacagca attggccaga ttaacggctt cattcttttt ttcatcactt ttcccatcct
     7981 tactactttt taactaattt cttattataa gcaaaaatgt ttggaagaac cataaacaca
     8041 acaggctgtt tgtctaaaaa acggtgcatc ttttattttt ttgtacgttc cgggcttttc
     8101 catgtcccgc ttccataacc taaaatcgtt ttgactgctg gaatgaaagt cgtcacgatc
     8161 gttatcgcgt tcactatcca gaacaaaagc atatatagtg gtgcgaaaag aaggtatttc
     8221 aattttcttc gtcgatcatc tacaatcagc gaagctagca actggaaaaa tcctgcgacc
     8281 atttcaaagc agatgaacac aaaactgaat gtaaaagtaa tataaatctg ttcgtagttt
     8341 ccttgatata cataatgccc gattaatcca gcaaaaagaa ctacggaaat acaaaagaat
     8401 attgaccaga caatactcaa tgtttgatca atgaagatga tcgttcttcc aatatgttta
     8461 aaaggatgga gcatcacttt tttgaaattc gtcagccaga cttctgttcc accttttgcc
     8521 caacgttttc tttgtcgata tagcatcttc aacgtcacag gtacttccat gaaaaagata
     8581 atctcaggag caaaaacaga taaccagtca tttaactgat gatcccaagc aatactgatg
     8641 tcttctgtcg ctcgatcttg tctgaatccg cccacatcga tcaaggcttc ttttcgatac
     8701 atcgtatttg ctccactgta cgcataaaga ccaccgaaaa cagctgactg tgttcgttta
     8761 atgatgccca cgatacttga gaattcaacc gtttgagatt ttgcaatcaa tttggtacga
     8821 ttttggacat ccatatttgc agtgaccgca gcaatatgtc ttgcaccagg gcgaatgaaa
     8881 taattcacat acctaatcag tgcatctggc tccggtaccg tgtctgcatc attgcttaaa
     8941 atcaatttcc cttttgcaaa ggcaagtccg atattaaacg cgtgggcttt tcctttattt
     9001 ttttcgatcc gaaccacacg aagattagcg tattttttca tcagtctagc taatatttct
     9061 ggtgtttggt ccgttgaacc atcgtctgtc actaatacct catagttgtg ataattcaat
     9121 ttcgtcatca aatactcgat tgtatcttcg atcacaattt cttcattatg ggctggcacc
     9181 atgatcgtga tgaacggttc gacactcaag ggaacatcta cccattcttt ttgcttatgt
     9241 ttgtataaaa acacataaca ccagacacca ataaaccata caaatccgcc taaaatcgga
     9301 taagaaacga gaaaaaatac ataatagaca aacaagtatc taagaataaa ttacctgtat
     9361 gggtgattga gatcatttca gttccacccc attttcttta tacaaatctg caatgaatgt
     9421 cgtatctaga tttttctctt cggagacaga ataaaaacgg accgtttctc tctcttcttt
     9481 tgttccaaaa cgttccgtat acacttcttc aagtaattct tgtctttttt tcaatttttc
     9541 atcttcatat tcaaaagatt tatttacttt tttgtataga tttcgattgt tccacctagt
     9601 caatacaaat gaggtaatca ggaaactgaa aaaaatcaat acaaaaaagc cgattaagaa
     9661 atacagcatt ttcttttctg ataaaaaagc gtacaaggaa aaatccttgg ctctttctgg
     9721 tctcgttaat gggaaaaaga cccatataaa aggaagaaca acgatcatcc atcctaaaag
     9781 agccactaac gtttgccaaa tctttaacca gtaattacct tttgaaaaat acttatcttt
     9841 caccaaaatt gatgcaactt cttctcttgt tacttttttc atatcctcac ctcacgtttt
     9901 tttgttacga cgtaggagtt taccatacat ttcatttctt ttcacataaa gtgttcctaa
     9961 atttttccta agaaaaagaa caaaacattt ctcatcagca aatgaaaaat attttgttct
    10021 ttttatcagt tgttcttttt attttttatc tcttaattat gttgctcttt ccaatacgac
    10081 aatgcttttc attcctctag atatcgccgt gtacatttgt ctgcgtcttc tttgctcatc
    10141 tccatcgttt tcaaatggat ataagattac atgatcaaat tccagtcctt ttgctagact
    10201 caaaggaatg atttggcagt tttctaatcc atatgaattt tttttaatcg attcttgtag
    10261 cgtttgggtt tcctcttctg tttgggtgat aactgccgct ttttctcctt ttagagaaac
    10321 caaaatctca gctagtattt tcaacaattc ttcagaggat cgaaaagagc ggtattcggg
    10381 ttcttttcct tgcggtcgga tcgatacgat gtcgatcttc tcttgatcca cagcataggt
    10441 tttgaaaagc tctgtgattg ctccgctaga acggtaacta tttctaagat cataccgatg
    10501 gatcggcaga tggtagtcgt cgaaacaacg cataatatca gcaaaagtag tggaagaatt
    10561 aaagattgcc tgattctcgt cacctactaa agtaaagtga gtttttggaa ataattcgat
    10621 caatagacct aattgtgcca gggtataatc ttgtacttca tcaatcaata gatagcgaaa
    10681 tgctggtaca tttacctttt caacaagtaa atgacgaata gctaatataa tgactgcttc
    10741 atcaacagta cgtgtgggtt gccaagcata agctgagcca tatcgttttt cataaattct
    10801 ttcaaacaac tgatgctcat ccacccatgc catttcttcg atttgtctgg ttatttttcg
    10861 atattttttt tgtaaaagtt tccttgcata agctacaata ctttgttccg aatcatcaga
    10921 aatcaattta ccgaataatt ccaactgcct atcttctgtc aacgagagta cctgattctg
    10981 cacagcagga ctttttgctt gtttcaaaag atgattcttc cattcgctca tcagtgcctt
    11041 tttcgttgct tgaagcctat cgatcaattt aggatgagca ggaactttct catatatttt
    11101 ttcgatcgtt tttttagaaa ggactgtttt tccattttta gtaagtccac ggataaaatt
    11161 cggatgatct aaaaacaaag gatctgattg ttttagatta tcgataaact ttttgctacg
    11221 caaacgttct gtctgctccg atacattttc tcccgtgata cgtttgaaat aagcctcttc
    11281 accttctatt tcctctgctg aaagctgtga aaccagctgt atgatggtta gattcagtgg
    11341 atttctttca cctaatgatg gtaggacatt cgaaatatag tcgataaacc gatgattagg
    11401 tgatagaatc agcaagtcat cggatgtcat cgtatctcga tataaataga gcagatacgc
    11461 aatacgctgc atgatagcag aggttttacc acttcctgct acgccattta ccaagatatt
    11521 ttgacttttc acatcccgaa tgattgtgtt ttgttccgat tggatcgtag cagtgatagc
    11581 cttcatttca ttggtctcat cttggcctaa gacttctaat aatacgtcat cttgaatagc
    11641 tacagacgta tcaaaaaact tgaccaaacg atctctttcc aaaagaaatt gtcgtctttc
    11701 tttaataacc gtatcgattt tattttgacg aaccgtataa gaagatggac caatcatgtt
    11761 attgtaaaaa agttcggcaa ttggtgaacg ccaatcataa acaagatttt catctgcatc
    11821 atctgtgaaa tttgctgtac cgatataaaa ggattctttt ggctcattat ccagaaagtc
    11881 tacaacgact tttccaaagt agggactttt caataaacgt tcgatctttg ctagctgatt
    11941 ttctgcgttt ttctgtttga tattcatctg atcgatttct ctatttttca tttcaatcat
    12001 cgaaaaagta tccaggttat ctaagtaact gtcaaaattc aatcgaagct cgccagccat
    12061 ttctttcaac gactggtttc cttctatttt tgccgtatcc atcgattccc gcagttcgtt
    12121 ttttttatca attagctttt gatacacgtt cttcagatat gcttcttcta actgtttttg
    12181 tgtgttgacc atacacattc tcctcttttt ttaaagtggt ggaaccgatt agtttacctc
    12241 ttttaagagt cgaatgcaag ctttttaacg agtatcattt gttaggatat atcagttctt
    12301 caggtcggac agtttgatat aaagaaaatg caccatcaag gttttttacg tggtagccag
    12361 actgcttcaa gattctttcc gctagatagc tacgtaatcc gctatgacag ctgacgatat
    12421 attcttgttg gctgtctaat tcatttaatc ggtcccgaag ttcattcaaa gggatatgtt
    12481 tggcttcctt gaatttgccc ttttccaatt cttctgctgt tcgcacatct aataaaattt
    12541 ttccttttgc tagttctgtc ggcaattcat gccattgtac ggtttcacta agcccttctg
    12601 caatgttcat tgctgcatat cctagcatgt ttactggatc tttggctgag ccaaatggcg
    12661 gtgcatacgt caattctaat tccggtaaat cgaagatcgt caaatgccct ttgattgctg
    12721 ttgctaaaat atcgatccgt ttatccacac cttttgcccc aacaccttgt gcaccataaa
    12781 tctcgccagt ctctggatgg aagatcaact tcagtaaaat atctgttgct tctggataat
    12841 agcttgcatg atcttttcct gatacatgaa tgacttcaaa agacagttcg ttttctttgg
    12901 ctgttctttc acttaaaccg gtggatgcgg cagtcattcc aaacgttcgt ataattgctg
    12961 ttccaatacc gccttgattt cttcgagaaa ttccggaaat cgtgtccgca acttgtctac
    13021 cttgacgatt ggctggtgaa gcaagagaaa taagtgcatc ttttccagtg atttgctgtt
    13081 ttacaacgat agcatccccc accgcataaa tatcaggaat attcgtttga taacgttcgt
    13141 ctactaggat acccccgcgt atgcctaatt cgattccagc ggtcactgct agctggttat
    13201 caggacgaac accaatggat aaaataacta aatcagcagg gatggtacgc ccatcatcca
    13261 gtcgcaatcg tttcccgtgg tcttcaaatc cagcgacagc atgagaagta atcacttcta
    13321 ctccttgatg agacaattct ttttctataa aagctgccat ttcttcatcc agtgttggaa
    13381 gaatatgtgg tgcttgttcg atcaccatga cttctaaacc tcttcttttt aggttttctg
    13441 ccatttccaa tccgatgaat cctgcaccaa cgatcacggc acgctttggc tgtttttcta
    13501 atgcatgcat cactttatcg atatctggta catttctaat agaaaaaaca ttgtcggctt
    13561 ctgttatccc tggaatcgat ggaacaactg gttttgcacc aggagataaa accaatgcat
    13621 cgtactgttc aatgtgtttt tgaccctcat gtacgacttc taccgttttg ttttcgggaa
    13681 agatggctgt cacttcatga tttggacgca catctaaaca aaaacgagcg gataatgatt
    13741 ctggcgtttg gacaagaaga ttttcccgtt cactgatttc tcccgaaaga taataaggca
    13801 agccgcaatt tgcaaaagaa acatacggtc ctttttcaaa aacaacgatt tctgcatctt
    13861 ccattaatcg gcgaagccgt gtagcagcag acattccacc tgctacacct ccgacaataa
    13921 cgattttcat cttatttccc tcctactgtt cgatcaaacc attgattcat gccaccacgt
    13981 acattcacta catcataacc agatttcttt aattctttag ctgcttgttt actcctcatc
    14041 cctgattgac aaatcacata gacggtcttt tctttgtctc catcatatct gtttatactt
    14101 tgtaatggaa catttttagc tccttttata tgcccgcccc ggtattctgc aggtgttcta
    14161 acatcaagta acgctggatt ttccaaaagc ttttccttta attctgggac tgttatcgat
    14221 tttattgatt gaaatagcaa attcattttc tgcacctcat ttacattttt ataaatacct
    14281 catggggtat attaaacagg agctttatta atatgtcaac cagaatgact cttcattttt
    14341 ttcactactt tttatatttg cataaaatac cgataagggg tattatctaa ttataaaaat
    14401 agaggagaat gtgttatgga gattgataat aaaaatatcc tgaaccgttt gaaacgtaca
    14461 gagggacaaa ttcgtggtat ccaaaaaatg atcgaagacg aaaaagaatg tatggatgtc
    14521 atcactcaat taagcgctgt tcgttctagt attgatcgcg taatgggcat gatcgttgca
    14581 gataatctga aaaattgctt tgagaaacct gatagtaatc cagaagaaca agcggctaaa
    14641 ctagaacaag cgatcaagat gattattaaa aagtaaagga atgatttcaa tgaaagtcgt
    14701 tttccatatt gatgaattag aaaaatggtc agaaacaggg aaaaacgtaa aaaacctgat
    14761 aaaagcttct ccagaaacga cgattgtcgt atctgtaaat ggcatcgcga ttaccggtta
    14821 tctagactct gccaatgcag aattcttaga tttgcaagga gtcgtttttc acgcttgcgc
    14881 caatgctatg cgtgctaatc atatttcaga aagttcttta cctgaacaag taatcgttgt
    14941 accagcaggt gtccttgatt tagttgaact gcaatcccaa ggttatgctt atatcaagcc
    15001 ttagtcagta gaggttgcga cagaagtgat cagcttcaag aaatttcggc ttatttttcg
    15061 aagaagctac ttctggaaaa ccgtgaatca aaggttgtga aaacgctgtc ctgcatcaag
    15121 taataagaac agccaaacaa aaatagcttc tcatattttt cgtttggttg ggcttattac
    15181 cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cggtttgacc
    15241 taagtattag agaaaaaaac gggaaaatag ctcctcatat tttgccgttt tttgagctaa
    15301 taccgaaggt cagctgattg aacaccgttt aaggaagaga ttgtgaaaaa gtcgcttagt
    15361 ttcgagtatt aagaaaaatt ttgtgaaaat ggatttctac atttttgcat aaatttggct
    15421 taataccgag aaactggctt ttgaataccg tttaaggaag aggttgtaaa atcagcggtt
    15481 tgacctgagt attagagaaa caaacggaaa aatagctgct catattttgc cgttttttga
    15541 gctaataccg aaggtcagct gattgaacac cgtttaccaa gaggttgtga gcctaccatt
    15601 taactccgaa tcacaagaaa taatttcaaa aacaacttct cctgtttttg gtttggttgg
    15661 cttattatcg agaagtagct actgggagtc tatttatcaa aaaaaggaat gcgacatagg
    15721 tcacacccct tttttatttt ctttgaaaca tctttcggta ctcgacagca tacaagacaa
    15781 aagattgtaa aagaccgtaa gtgatgacgg caaaaatgac tctcacccac agcgttggta
    15841 attctgggaa aagcagcaaa aggatcatgc caacatagat gacagcagtc gctactttgc
    15901 cataccactt cgctccactt aatttgatat ttttagatag cccgattaat ccagctacaa
    15961 acataaagcc ttcttttatc aagaatagac caaacaacag ccaaagccac ggtcgatacc
    16021 atgccataga caaaatcaga aaaagttgtg tcaatttatc tgcaaacgga tcaagcacct
    16081 tccctaagtc tgttacctga ttatatctcc gagcaatgac cccgtctaga taatcagaca
    16141 aacctgagac aacaatgacc gctcctgcta tataaaatga ctgtctcagc acaaaataaa
    16201 gataaatcgg cagcaaaaaa atacggaata tcgataaaag attcggaatc gtttgtattt
    16261 ctttacgcca gtctgtgctt aatttcattt tatcgcccta cctcctcagc actcttttct
    16321 ttttatttta gcttacacgg ggaataaaac caatgatttc actgttttta tttttcttta
    16381 cagccactga tattatgagc taatttgaga taatcacttg ctgttttcat cgtttactct
    16441 tctttcttcc tgtcgtttgc gcatttcttt tactagtact ttatgagctt tttttgatcc
    16501 tcgctctagg tcacgctgcc gtttacgtgt tttataatcg ataaacagac tgtctaaatc
    16561 gtaacctgtc gggtacagat cttctgctga acgcaccaga cgaacacgct gacgaggcac
    16621 gtggattatt tttttgtcta aatatacatc aatcgtatct tcacctatat cttcataaac
    16681 taacgcttct ttttggtatt cattgacaaa tatccgatca ccttttgcaa agagctggcg
    16741 ttctttttta ttctccttcc tatttgtaac tgctgtctta ggaagaaatt cttttttctg
    16801 tgtccgatag gctttttccg ccaagtaacg ctctgcttga tggatcaatt tcatcgacat
    16861 atccatttta tgagcaatcc acaaagcttg gctttctcct gtttctccaa cccgcaactg
    16921 atatttagga cgaagtgctt cccgatcgaa agccatagca gccggcacaa aatcttcatg
    16981 atctccggcg aatttcttga tttctccata atgggtcgtg gcaatgatca atgccccttg
    17041 ctcatacata gattccatga ttgcgatcgc tagtgcagcc ccttcatttg gctctgttcc
    17101 actgccaatc tcatctaaaa gtaccaacgt attccttttt atctttttca aaatagcagc
    17161 gatgttttgc atatgaccag aaaatgtact caacgcattt tctaaatttt ggtggtcacc
    17221 gacatcgacg aaaatctcat caaagacagc aagctctgtc ccttcttttg ccggaacttg
    17281 cataccgaac atcgccatca aagtcaataa accgactgtc ttcaacacga ctgttttgcc
    17341 tccggcattt gctccggtga taatcaagcc tcgatagtct ttaccgattt caacatccaa
    17401 tggtaccgca tgctcaggta aaaatggatg tctgccttgc ttgataatga tatgttccga
    17461 tttattgatt ctaggtgtgc ttccattgat ttctcggcta aattttgccc gagcaaagat
    17521 gatatctaac accgtgatcg tttcgatcaa taaatcgatc acttcttcgt tttctgcgat
    17581 agcaccagtc aatgccgcca ggacttgata ttcttcactg atttcttcag cttttaacaa
    17641 ctgatagtgt tcattcaatt tagaaatcac agttggttca ataaaaaccg tcgttctttt
    17701 atttgattca tcgataatcg ttccatctat tttattttta tagctggctt tgataggaat
    17761 cgtatagtat tcgccttttt gaacaatcat tgcttcttgg atcatttctt tgtttttcgg
    17821 atgacgcaag aatttcagta attttgactg gatttctttt tcgattagtt gtaattgttt
    17881 gcgtacttta cgcaaattac gtgacgcgtc gtcactgact ttattatttt ttattttctg
    17941 gtcaatcaac tcttctacat ttatcaaatc aggaagatgt ttgctataag caaacaacaa
    18001 gggtgtctgg tattgattct tatcaaaaaa ctttgtgatc atccgactgc ttctaagaaa
    18061 atccgcatat tcgatcaaat cggttggctg cagaatcaac ccttttttga cttgttctgt
    18121 cagtgcatcg atcctcggca accccataaa tggaacatgc tgactgcttt ctaagatcaa
    18181 gcgtgcttct cttgtttctt cttgacgatc caagacagtt tgtaggtttg tcgagaccgt
    18241 catatcgctg attcgttttt tgctatagtt tccaattgct cttgcttgga tttccttttt
    18301 gatccgatca aattgtaatt gactaatcat ttcgttgtcc atattctctc cttttcttga
    18361 agagatacct gtcccaaaat aaataagggc ctagagataa ataaaaggat agaacacagc
    18421 ttaaaaaagt catgtcctat ccttattaga atacaaagat attgttttcc tgacaggtac
    18481 cgtttcataa gcacaccaaa taaactttgt ttattggcta ttcgacttac tgattagata
    18541 ccagctacac tcacaaaaca taccccattc ttctaggtct tatttatttt ccacttaact
    18601 atagcaagac acattttggt tgtcaaactt tagtatgttt tatagattta caccattaat
    18661 gaatagacac ctaccgcgat aattgctccg atcattggac ctgcgattgg tacccatgaa
    18721 tatgcccaat ctgaagttcc tttattagct attggcaata ccgcatgtgc taatcgagga
    18781 ccaaagtccc gagcagggtt gattgcatat cctgttggcc ctcctaatga aagaccgatg
    18841 gctaagatca atccacctac agcgaatacg ttcgttccag gtgcaaattc gttttgtcca
    18901 aaagctagca acccaagaac taatacaaat gtaccaatta attctgtgat cacatttgct
    18961 ggataattcc gtatagcagg acctgttgca aacgttccta aaatcgcgct ttcatctttc
    19021 gttgcattcc aatgtggcaa ataagaaagc caaacaagaa tggcccctgc aaaagctcct
    19081 aaaacttgcg caatgatgaa aggaacgaca agattccaac taattgcccc tgtcatcgcc
    19141 atagctagag agactgcagg attcaaatga gctgggccca tgaatccaga aatatagaca
    19201 gccattgtta cagcgagtcc ccaaccaaat gcgatgacaa tccagccaga agcctgtgct
    19261 ttacttttgt ttaaattcac agccgcacac acaccatctc caagcaaaac taggatcagt
    19321 gtcccaataa actctcccat gatttgagtc atatcactgt tcatttattt ctcccccttt
    19381 agttcccgca gatctgattc tgcaatcaat gcttctaaac gtttagtctg ttgcgccttc
    19441 tcctcttctg tccattcaaa atatccagca attgcatcga tcacaggttg cttgatctca
    19501 tcaagctgat ctcgttcaaa caacagatga tttgtacgac gaatgagata atcgcctggg
    19561 gccaaaacca tttcttcttc taaaccataa cgtaaccgag ctgactcagc gagactcaaa
    19621 ccaggataag gtgccatttc ttttgctaat tcaaagatac gtcgggcatt agtcccgtaa
    19681 aaatcagcga tataagtagc atcttcttcc tctaaaccgg ctgctactcc ttcttttgct
    19741 aattctgtca ctgtttcttc taatttagtt ggatcgaagt ttccacctga aatctgatat
    19801 tttttagagt cgatttcttt cgtctctatt ccgtattctt cttttaataa ttggcgtatc
    19861 aatcgcaaag ctccttctgc catcttacgg taatcggtga ttttcccacc agataaagtc
    19921 agcaaaccat ctggttctct ttctaaagaa ctacctctgg aaatcgtaga aggtgctttt
    19981 tcatcacggc tgttttccaa atggttcaac acgtcttcta cttcagcacg agaaactttg
    20041 ttttccttat attcacttac tgtatcgaca actttattga agctcttgtc tgaaatcgat
    20101 ccattatctc caccattgta atcagaacca gaattaccaa tcaacagtgg acgaagtcct
    20161 gcccagcttg cttcgatatc attcaatgtg atatttgctt ctggatagcg atgattgatc
    20221 acatccaata gataatctac atcttcttgt gtgactttcg gatccgtaaa atctccttgg
    20281 taatccgtat ctgtcgtacc aaagtaagtc ttgttttctc ttggaatagc aaaaaccatc
    20341 cgcccatctt gctttcctgt atcgaagtat gtgggttgcg gtacaggcag ttttttcgca
    20401 tctacaacta aatggatccc tttagttgga cgcattttag gagagactgg acgcgtaaaa
    20461 ttcaagtttc gtactttatc tacccaagga ccactcgtat tgatcactaa ttttgctttg
    20521 atttcgatca cttcatctgt cagcaggtca cgagctttga cgccaacgat ttgatcgcca
    20581 tcatataaaa agccaaccgc tttcatttta cttactagat aagccccatc ttcggcagcc
    20641 tttttgatat tatcaatcac taaacgggca tcattgttgc ggaaatctag atagacaccc
    20701 gcgcctttta gcccttcttt tttcaaaaac ggttctcttt ccaatacttc ttctggtgtc
    20761 agcgtataat tctcatattt ggttcctgtc acatttgcca gtttgtcata aaggtccatt
    20821 gctactttga ccgagaacat attgaaggtt gttgctcctt cgccctcata gattggcaaa
    20881 agcattggat ctggttttgg gatgtgtggg gcaatacttt gtacgactgc acgttcacca
    20941 actgtgtcag ctactacttc cacatcaaat gtcttcagat aacgaatacc gccatgaaca
    21001 agtttggtcg agcgagagga agtcccttct gcaaaatcct gcatttcgat caatcctgtt
    21061 ttgattcctg atgctgctga ctggactgcc acaccggcac cagtgattcc gccaccgatg
    21121 atcaacagat ccaattcttc ttgctgcatt ttttgaatgc tatcttgtct tgtcttgttt
    21181 gaaaacatat ggatttcctc cttattcaaa tgcttgggta gcttttactg cttttttcca
    21241 accgttgtaa agtttttctc ggcgctcttc ctccatttga acttcaaata gcttgccagc
    21301 ttcataaaat tcgcggatct cgtctgtatc tttccaataa ccgacagcta atcctgctaa
    21361 aaatgctgcc cctagcgccg ttgtttccaa gttttctgct cgttggatcg gtacattcaa
    21421 gatatctgtt tggaattgca tcagatattc attgtttgct gcaccgccat ctactttcaa
    21481 tacaggaatc ttgattccag tgtcatcttg catcgtatcg atgatatctc tcacttgata
    21541 agcaatcgac tgtaaagtag ctttaatgat atcttctttt gtcgtaccac gtgtcaagcc
    21601 aaacatagcc cctcgtgctg cctgatccca ataaggagca cctagcccaa cgaatgcagg
    21661 aacaacataa acctcatctt cgcttgtaga gcgtttcgct gcgtcttctg aatcgctagc
    21721 tttttgcaac atttgaagtc catctctcag ccattgaatc gaagaaccag ccacaaaaat
    21781 acttccttct aaagcatagt aaacctttcc atttatgccg taaccaattg tcgtcaataa
    21841 attatttttg gataattgcg gtttttcacc ggtattcatg acaataaatg aacctgtacc
    21901 ataagtattc ttgaccatgc ctggttcgaa tgccatttgt ccgaataatg cggcttgctg
    21961 gtctcctgcc atccctgaaa ttggtgcttc gcttccataa aaatgataac cttgcgtata
    22021 accataaact tctgagttag aagtgacttt tggtaacatc actcgaggaa tatttagtaa
    22081 atctaaaatc tcctgatccc aatctagctg atggatattg aacaacatcg tccgactggc
    22141 attcgagtaa tcagtaacat gtgcttgtcc atcggtcagt ttccatacta accaagaatc
    22201 gatcgttcca aacatcaatt cgcctttttc agctcgttct tgcgcgcctt ctacatgatc
    22261 aagaatccag cgtatcttcg ttgctgagaa ataggcatcg atcactaacc ctgttttttc
    22321 gtggaaaaat tcttgatagc cttcattttt taagttatct gcaattccag ctgactgtct
    22381 ggattgccaa acaactgcat gatagattgg acgtcctgta tctttttccc atattactgt
    22441 tgtttcacgc tggttcgtta tgccaatccc agcaatctga tttggtttga tccctgattc
    22501 gatgaacgct ccggcaataa ctgactgtac agaattccag atttcatttg catcatgttc
    22561 cacccaactt tcttgcggga aatattgcgt gaactctttt tgagaactgc cgatgtgtct
    22621 tcctttcttg tcaaagataa ttgctcgtga gctagttgta ccttggtcaa tcgccatgat
    22681 atacgttgat tctgtcattg tgattccctc cattatatgc aaacgctttc tttaactact
    22741 cttatcatac tactgtttta aaaaaagaaa ctgtcaaaat tttattcttt ttttcttttt
    22801 ttgacagttt tttattatga agctattttt taatcaattc ttttcttttt tcagcgtctc
    22861 tattcttctt ttcaatcgaa taatgtcgta aggtgacgca aattctgaca gataatattc
    22921 acactgtttt ttgtttctct ggcaaaatgt gatcaccaaa tcatattctt taccttcttc
    22981 ataacttcca atctcaatac cgccgattcc cctcagttct ctgatcagat attgttgaaa
    23041 agcaatacga taaatcggca gactttctaa atcgattcca atcactactg gttttgcaat
    23101 ataaaaatcg atcatcaaaa gaacattggc atactggatc atcaggtaac taagctcact
    23161 atcttcagct cttttcctcg gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa
    23221 ctgtgttaag gaatgcccga gcattttctg ctgctgttcc aaaagccgat cacgctcata
    23281 gacttcgatc tttccaacaa agaaaaacca ttcattgttg atctgggaaa tttggtagcc
    23341 tactgccttt tcttctttga ttttcaaacg acgaggacga taatgccata acatcgtttc
    23401 ccgaatgtaa gtatctaaaa cagcggttgg taactttttg ctccgtgtca aatcatactg
    23461 ataaaatatt tcttcgtcta ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt
    23521 ttcgtataca ttcagttctg ctgctgttcg actaagatgc atcacgatga ttggatcgat
    23581 tgcttgataa agccggtccg actgatagag taatttcttt tctttcaaag ttgcgtacgt
    23641 acttttttca ttcaatagtc tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc
    23701 tgctgatgcc gtaaacgttg tttctaatac acggtccaat cctagaatga acggtctttt
    23761 ttctggtgta ttttgcaaaa aaagcggtcg ctgatcttcc gggatatatt ggaatagttc
    23821 ataataaaaa tatcgtatct gtagctcttc tccgtataat tgaccgtttt tgatttgtaa
    23881 tccaaattcc tctaagagct ggttcaattc tttgatcttt cgaaaaacag aagactcact
    23941 gatattaaac gctgtcgcta atcgaacgat ggatacttgc tgatgttcta ataaatagac
    24001 gagcatctta aacttcaagc tttcctgcaa taaaaacaat agaatttttt ccaaacttac
    24061 atcttcgtcc aacacaagca gcgcctgata ctcatttcgc tgtacttcta cttttttttc
    24121 catcattcgg ccaatcaatt caatatcttc caaatattga tcaaacgagg cttttgacag
    24181 ttggacatgc tctctcaaac cattgataga ggctgttccc ccttgcagaa caaggtttcg
    24241 caaaatcagg atctgtaact gctcttttct ttctaataat gcttcaatct tcataggcag
    24301 tattcgccac ctttgaccac accatatctg gcacacggta ttctggctgg aaaaatagct
    24361 catgttcttc taactgttca aacgtccatc cttcttctac tgcaattgtc agttggtcaa
    24421 gaaggtggaa catctctttt ttgctttgga gctgtgctcc aagaatcttt ttttcagaat
    24481 caaatagaaa tttgagttta actttgcttt gatttattgc tgtaagcttg tagtctacgt
    24541 atcctgtttc aattttttgg ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc
    24601 ctaagaaata gccaaaccac tgattgccga ctggacgata ggttccctga tcctttgtct
    24661 gtgagccact aattgtcttg ctgacatttc gtgctgtacg gacagcgtta tttactagag
    24721 aaacatacat tttttcattt gttggacgaa aagttacttg gatcgcatcg ccaatcgcat
    24781 aaacgtcttt ttgagacgtt tgcaagtagt catctaccca gatcgttcca tcatcattca
    24841 atgttaattg ttcttcccac atctgattgt cggggcgaga atgattagcc agcaaaacaa
    24901 gatcatagtt ctcctttctt ttttccgtct ctacaatagc ggtttcattt tcggtcaaag
    24961 acacaacttg ttcttctaaa aaaagtgaaa gcccttgatt tttcatttcc tttactaacg
    25021 gttctgtcat ttcaggatct agataacgaa aaagaattga ctttcggctt tcgtaaagat
    25081 ggatttgttt ttttcttgat gccaaccctt cagctatttc gattcctgct tgtcctgcac
    25141 caataattgc aattttcttt gcattcatga tcttttgctg aagagccgtt acgttttgta
    25201 tctttttaac tgggtgaata agcgttgatt ctgcagaaat atttcgaaac ttttgatttg
    25261 aaccagtcgc tagaatcaaa cgatcaaaat ccaatttttt tcctcctgct aaaacgactt
    25321 ttttttcttt caatcctatt actgtctttt ctgtgtaaac atggattcga tagcggtcag
    25381 tcagttcttc tttcgtgatc caagaaagtt gtttttcact ggtaaccttt ccttttaata
    25441 tcaaattgaa cccgctggga atccatccta acttcgattg cttttcaatc aaatatattt
    25501 cacttgttgg catcaattgg cgaagataaa tagccgcatg aatccctgca aacgacccgc
    25561 cgacaattac aatgttcatc tgcattcctc tttcttataa ggtgttctac tgctaacttc
    25621 tcaatcagaa aacacagtat tcataactcg ctttcaacct tatgatacat agcgatgcag
    25681 cagaatcaac cctctccatc ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg
    25741 gacctaattg aagagtatca tttctgaaga gatgtttcaa aaaatgtact cttataaaag
    25801 aaaaaaactc gccttttacg tacctctgtc caaataggaa acagaaaaga acgaaaagac
    25861 gagttttagc gtgtgtaatg taatagttag aatagacaca tcatttattt ttaattcttt
    25921 aatgcttcaa caactgcttc gcctaattga tgagcagatt gaggattttg ccctgtaaga
    25981 agccgcccat caatttgaac cacttctgaa aaagctgctc cagactgata gtttgctcct
    26041 ttttctttca aagcatcttc tgctaagaat ggtacttcat tcgttgtacc atttgcttcc
    26101 tcttcttcat tactgaaccc agtcactgta cgtccagcta aaatagattt tccagactca
    26161 tctgtaactg gcaacaatcc tacaacaccg tgacaaacag ctgagaccag cccttcattt
    26221 cgatagattt gtgaagcgac tttcccaaga tctattgctt ctggaaaatc ccacattgtt
    26281 ccgtggccac cagcataata aaccgctgca taatcatttg catccacatc atcaggacgt
    26341 aaggtatttc ccaaggcccg atttctgaaa tcttcatctt cataataggc ccaatcgatt
    26401 tcatccatat tcaaaacact ttgcggttct aaaggtacat atccgccttt aggactgaca
    26461 aaatctgcat cgatcccatt ttttactaat acatcataaa aatgagtcag ttcacttaac
    26521 cacaaacctg taggacgctc gatcttttcg tacttttcta catttgttaa tacgatcaat
    26581 actttcattt tcccatcctc tttctataaa aattgcgtta ttgataacgt gctttcaaga
    26641 aattatataa tgggcttggc aaataaagct ctttatatgc ttcttcaaaa ggtttcgtta
    26701 caaattcatc ctcacggttt tccaataatt tctctgccca gtcaggatcg acgatcattt
    26761 gctgtccaat tgctgcaagt tctgcatatt tcaatacctg ctctacatct tcgcgattgc
    26821 ggatcccacc gacaccaact aaagggatct ttccttgaat cgtgttatgg accacttcaa
    26881 gaatcgattt atcgttgtat ttatcagaac gagctattcg atcatacata tttaatgaaa
    26941 catgcagata atctagtttt gactcacgta atcgttcaag aagccagatc gtgtcttcaa
    27001 aacggatacc tggtgtttca tattcttctg gtgagaaacg ataccctacg atgaatggtt
    27061 ttttagcata ttgttcaacc gctgagaaaa ccgttgttaa taattcttca ataaaacgat
    27121 aacgattttc caagctaccg ccccattgat cctctcgtct attcgaatgc ggagagaaaa
    27181 attgttggat caaatacgta tttgctccat gcaactcgat tccatcaaat cctgattcaa
    27241 tcgctctacg tgtggctttg ccaaatgctt ggattgtttc aacgatttct tcttcactca
    27301 ttgctcgtgg tgtttccgca tcaggacgtt ttgctgcaac tgcacttgca gatacaggct
    27361 gttctccaga taaagttgct cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg
    27421 cttttgcgcc ttctccttgg attgctttcg ctagctcact taatcgagga atcatttcat
    27481 catgagcgat gctgagttca cctggccagc cttttccgat atcttggaca tttgcagcac
    27541 cagtaataac agcaccaaga cctttagaac gttgtttata ataagtgatc tcatcttcgg
    27601 tgatcactcc attgaaaaag ctgagttgtg tcgtcatagg cgacagcatc aatcggttct
    27661 tcaattgaac tcctgaaggt aaagtaattg attgatcaaa ttgtgacatt gtcattcctc
    27721 ccataattcg ctctgattaa cctatcgaga atcatctatc attaaaccac tcgattagtt
    27781 gtacatgcaa ctaatttgct taaaaaattt tatttttttt gtaacttatc tgttgcatca
    27841 ttcattgtct gggctaattc tttacttgct tcatttccca aaactgcttt gtatgactga
    27901 tgaagttctt cccagctacc atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga
    27961 taaactaatg acaagcgacc ttcgctaact gttttgacgt atcctttata ttctaatttt
    28021 tcaacaaaac gagcaatcgt tgatggggcg gaatagagca tttgtgcaat atctttttgt
    28081 ggtattccat ctttttcttt taatatcaat aaaataaaac cataagttgg tgccagacct
    28141 gtccttctaa acgcttcatc agctaattta ttcaacatgc gatctagctt tttcacgcta
    28201 aagaaaagac attcatctaa cgctttttca attggctttt ccatttcatc ccctccattg
    28261 gcgtacatat catagtatga tttttatttt ttgtcaaaaa acacggaata atgtccgcct
    28321 cctctgtcta gatttgaaaa aaagaagtag ccatcttcaa agaatttcgg cttgttttcg
    28381 acaaaactcc ttctggaaca ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag
    28441 tatgaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac
    28501 cgaaaaactg gcttttgaac accgtttata tcagaggttg tgaaaaagcc gtcctgcatc
    28561 aagtaataag aacagccaaa caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat
    28621 gccgatggtc agctgattgt acaccgttta ttgagaggtc gtgagcctgc cgttgaactc
    28681 cgagtcacaa ggagcaattt caaaaaacag cttctcatgt tttatgacta tggtgacttg
    28741 tgatcgagga gttggcagga gaatactgtt tattcgtaaa gaaggggctg tgacaagact
    28801 tttgtcacag tcccttattt ccttttatta taggatttcg ccatttgatt ggatgacttg
    28861 tctgtaccaa tcaaatgatt ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc
    28921 ttgatcaaca tagatatatc cataacgttt tgacatttgt gaagtagaag cgctgacgat
    28981 atcaatgcag ccccacgacg tataaccaaa taaatctact ccatctttga tagcttcctt
    29041 catctgctca atatgctgtt tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc
    29101 ttcttctact ttgtctatag ctccgagtcc gttttcagct atgaacagtg gtttttgata
    29161 cctatcatat aattgattca aagcaattct caaaccctta ggatcaatct gccagcccca
    29221 ttcacttgat ggaagatacg gatttttaat tcctccagtt attaagtttc cttggacccc
    29281 ttcccaattt tctggatgag cactgactgc actagacatg tagtagctaa aagaaacaaa
    29341 atcaacaggg taggctgcta agatttcttc atcacctact tctattttta agttgacacc
    29401 tttttcttca aacattcgtt gcgtatacgc tggataatat cctcttgctt gaacatcact
    29461 aaagaacaaa gaagttcttt gctgctcaat tgttttttga atatcatccg gatcacaaga
    29521 atacggaaca agaagctgat agctgatcat acaaccgact tgtgaacctt tgataatttc
    29581 atgacaagct tttgtagcta aagcacttgc tataaactga tggtgtgcac cttggtattt
    29641 cgcttgttcc aaatggggat ggttttcttc aattactcct acgctaacaa aagggtgatg
    29701 ttttacacag ttgatctcat tgaaagtcat ccaatatttt actttatttt tatagcgttt
    29761 gaatactgtt tcagcgaaac gaacgtaaaa ctgaataagc tttcgattag gccatccacc
    29821 atattctgta accaaatata acggcatttc ataatgggaa agagaaacga ctggctcaat
    29881 cccatatttt agacattcgt caaacacttg atcataaaaa aataatcccg cttcattggg
    29941 tgtttcttca tcaccatgag gaaaaattct tgtccatgcg atagacatcc gatagcattt
    30001 aaaacccatt tcagcaaata aacggatgtc ttctttaaag tgatgataga aatcatttcc
    30061 gtgtctttta ggataataat acttgctgtt gggatcctgc gccgctttta cttgctgatg
    30121 cgtcatcata tgccattgat ctgtccattt ttctttaggt aaatcgctgt cgaatgtata
    30181 cacatcagac acagataatc ctttttcatc tacatcccat gcaccttcac attgattggc
    30241 cgctgtcgct ccgccccata aaaatccttc tggaaatttt ttcatatcga tccccctaaa
    30301 cttgctagtt ttcttgtttg attatttttc ttatgactag taatgatatt tttgaatcta
    30361 ctactatttg taacaataac cattgtggtc ggatcatatc cctctttgat taattcttgc
    30421 aaatctactt ccatcaataa atcgccttta cgtacttttt ttccatcaga tactgcactt
    30481 tgaaagtatt tgccatttaa attcactgta tcaataccga tatggatcag tatctctgct
    30541 ccttcttcac tgactacccc aattgcatgt ttcgttggaa aaactgttga aacttccccg
    30601 tttacaggac tgtagatttt tccgtccgtt ggaatgatcg caaacccttc tcctaaagat
    30661 ccactagcaa atgttggatc gtttacttca gataaatcaa ttatttcacc tgctgcagga
    30721 gtacttaaca ctatatcctt tgaggattct tcttcgattt catccttgta gccgaaaaga
    30781 aaagttagca tacaggaact gatcatcgct aagaaataac tagcaactag ccaaacaaat
    30841 ccttcaccaa tataaatcgg gaaagacaaa atacttcttg tagcaactgc caaagcaccg
    30901 gctcctgcgg caccagctac tccaccaaat atagctccac atatacaagc aatgtaaaat
    30961 ggtcgtttat atttaagagt cacaccataa acagcaggtt cagtgattcc aaataatgct
    31021 gtgataaatg cagataaaga taaacttttt actttcttat ttttcgtttt caaaaatacc
    31081 ccaagtactg ctcctgcttg cgcagctacg gcagtcatac atgctggacc tagcgtatca
    31141 cgaccgtaga aagaaagatt gttggtcatt actggtacaa gtccccaatg taacccaaaa
    31201 atcactaaca cttgccaaaa tccgccgata acagcaccag ataaaacagg gctaaagtta
    31261 taaacagcta aatatccagc tgcaatcagc tgacttgcat aatctgcaat tggacctaca
    31321 actaataaag taattgggac catcacgatg atacaaatga atggtgtcaa taaatttcga
    31381 atactttcgt gcaagctttt gcgaatctta ggctcaatat aagaaagaac ccatacagct
    31441 aaaattgctg gaataaccgt acttgtatac cttgctagaa tcacgggcat ccctagaaat
    31501 cgcaaggtta tgctatcact atatgcactg acaatcgtag gataaacgag agctgctgca
    31561 ataaccatag cgatgaaacg atctgtattg aacttttttg cagcagtata tgccaaaaat
    31621 ataggcaaga aatagaaaaa tgcatcagct gctgcattca aaatgatata agttcctgat
    31681 tcagtagtta ataaattcaa actcgtacat aaggcgagta ctcctttgat caaaccactt
    31741 cctgccaaga cgcctaaagt cggggcaaaa acagcggcga tcatgtctat aaaagagttt
    31801 aaaatgcctt ttttctcagt tcctctcttt ccactagatg catcatcttc tagtttcatt
    31861 tgtttggata atacgtcata cacttcattt actgcatttc ctactacgac ttgaaacatt
    31921 cctccagctt cgactacagt gatcacacct tctagttgct ctaccttctc cttatctgcc
    31981 ttcttattat cttttagggt aaagcgtaac cttgttgcac aatggtaaac attgctgaca
    32041 ttttcttcgc ctccgacaaa tcggacaata tctttcgcta attgtgaata gtccattttt
    32101 acttccccct tgttaattaa tgccgtagca atccttctta caagataagt atagttctta
    32161 gaaaacggtt tctccattca attagtaccc cttataatag cgaacgaaac tgtacaatag
    32221 tgtgtatggt ttacttagcc gagcaaatca ctcactgctt gaaagaaaac aagtatatcg
    32281 tttctcttat agtcaaagga gacaaaaaaa acacgcttga tcttcctttg ctgatgtata
    32341 agtggtatct caagctgata ctctgtgatt tttgtttcta cttggctttt gataagacag
    32401 cacaacaaag tctcggcatt taacggaagc ataactaggt cagaactagg agagattagt
    32461 tgctgaatgg aatctatctt tttatggatt tttatttgat tttcttcttt gtagtaatga
    32521 tcaagcagtt ttgttttctt tttttcttca actatggtca gcttttcacg caaaatctga
    32581 taaatctccc aagattcaaa ctcatctaga acactaggta gataaatcaa atcatcccac
    32641 tcaaaaataa gctcttgctc agtagtcaaa attgcatcat attccgttat tttgtttgat
    32701 tgatttttga aaagatacac tggttctatc cggcattctt tgatccacat gttcatactt
    32761 tgttgaatct gtttttttag tgtattcaaa atactaattg gctggttact aacaatcagt
    32821 atctcccctt gttttcgaaa aggattcaac gcttcggcaa gatacaaaac aaaataagcg
    32881 ctttcagcta aattgggacg taattgaaaa tgtattggga agaaacgccg aattaaataa
    32941 atttctaaag gataacggga gatgatcttt tgtacaaaat gattcatggt attatgtccg
    33001 gcatccatcc tcgttttcat ttgtttgata tgggttagca aaaattctgt ttcatggaac
    33061 aataacgaaa caggaagatc taaaaaacga ataatcgttt gctcaaatgc tcttagattc
    33121 ttctctattt ctttgttttt ttcatcatta atcatcaaat aatttaactc taatagtcga
    33181 ttttcaattg cttgtttttc ttgattggaa aaagaatagg gtagttctga tgataacttt
    33241 tttgtgaggg tctctaccat cggaataaat aatgtatacc tgtttgtttt ttctaaaagt
    33301 tcttctcttc gaagaatggt caaaacgaga taccgagaaa atcgtatgaa gtcctctcct
    33361 gaaacaatat aattatatga tataagaacc tcttttaatg tcttttggac agtcggcatg
    33421 atattggaaa aaattgtact gatttgttga ggcaaccttg cgtgttggat caccgtttct
    33481 gttccaacaa gagataagaa aattcttttt gaattttctt cagcaatgat ttttagtcct
    33541 tttgtcgacg aaacttccag ttctaattta ggattgcgct cgatccaacg caaaatcgtt
    33601 tttatttgta atgatacggc tgtttttgaa agaaagaaaa tgtctgctat cttttgcata
    33661 ctgacatacc ctttttgaaa caataagtac gtcacaatag ttgaagctcg gaaatccatg
    33721 ctggaataga ttttatgttt cgtcacctct tgtatgattt gctgtttagt ttcagattca
    33781 attgattcta aaaaataacc tgaacgttta tttgactgga taatgatttt cttctcgtag
    33841 atagcaagca tatttttgat ttcagtttgg atcgttttct tgttggtacc taaagcttca
    33901 gctaattctt cgcttgttat ccaatgattc ttatttctta atactacaag aatttggagt
    33961 tgtcgtttat ttaagtccat ttgcttacac ctcctctttg ttataaacat acaggaaaaa
    34021 cctctcaaat gacaatagtc acttgcagag gtttaaaaat catttgaaga aagatctata
    34081 ttcaaggttt ttaccattag actggataat ttgtttataa tattcaaaac tatctttttt
    34141 caatctccta aagcttccgc tcccatcatt ttgtctatcc acataaataa agccatatcg
    34201 cttcctcatt tcccctgttc ctgccgatac gatatcgatt ggtccccacc ataagtaacc
    34261 aatgatttgg caaccatctt ttattgcctc agcaatttcc tgaagatgta tttttagata
    34321 ctcacaacgt tttggatcgt ggattcgatt caattcatct ggtgcttcat ctagtccaat
    34381 gccattttca actatgaata atggtaattc ataacggtct gtcaattcat tcatgacata
    34441 acgaaaccct agaggatcaa ttggccaact ccaaggttcg ggagattttt ccttcaaaaa
    34501 gggattatcg aaaccatttg ctccgccagt atctacgcgc atttctgctt cttttttata
    34561 gacagcgcta cgataataac taaatgcaat ataatctgcc gtgttagctt gcaagcactg
    34621 ttcgtctcca tctaacatta ctggtgcaca gtcttcttcc ttccatgtcc tgtaaatata
    34681 agctggataa tgccctcggc acatgacatc ggagaaaaac catgaattgc gtttaaattg
    34741 cagtgaacca aatacattct ctggatcaca gtcatatgca taagtcgcta atgcagaaaa
    34801 gctacacata atacccatct tagcatttgg atcgattttc tttaataatt gagtagcacg
    34861 ggcgttcgct agaaacatat gatgacaggc ttgataaatc actttttttg ttagtgtagc
    34921 aatgggttca agtttatctc tagcagggaa tgtaaccaat ccagcagctg cataaggcat
    34981 acgaaaagca ttattgatct cattaaaggt catccaatat ttcactttcc cttggtagcg
    35041 ttcaaagacg gtctgcgcat aaaattcaaa aaagtcgatt aattttcgat tttgccaccc
    35101 cccatactct cctaccaatg ctaagggggt ttcataatga ctaagagtaa ttactggctc
    35161 gatatcatat cgatttaatg tattgattag tctgtcataa aatacgagac cagcctcatt
    35221 tggcttctgt tcgtctcctc ttggaaatat tcgtgcccat gagatgcttg ttcgaaaagc
    35281 ttttaaacct aactccttca gtaaacgaat atcctcttca aatcgatgat aaaaatcaat
    35341 cgcttcatga gaaagataat ggagtgattc gtctaaagca atctcccaaa tctttttttc
    35401 ttcattccac tgaatactag gatgctggtt catgatcccg ataagcgtat ctgtcacatt
    35461 aggtaatttg ccgtcttcca accatgcacc ttcacattga ttagcagcta ctgctccgcc
    35521 ccataaaaaa ttttctggaa atatttcctg atccataact atctctcctc gctatcaaca
    35581 atttcttact tgaagtatag ctcgattcat caaaacaaga tattctaact atttcggcag
    35641 aagagaagaa aaaaattgaa tgctactctt catttttaat atttatatat aacaaataca
    35701 taagacgatc cccgaaagaa aacactcgtt aaccatctaa gcaaacgatt tattttactt
    35761 ccaacacaaa tgcttcataa ggtcgtaaat caactttgcc gtctggtaac tgtttcagag
    35821 gataatttga cacaataacc cttaaagctt cacgtggaac agtgaacgtc tgcttttcac
    35881 tggacatatt acaaataacg agccattgct ggtcttcata tatgcgagaa taggcaaaga
    35941 catgctcagg cgtcttttcc actaattcat agtctcccca gacgatgatg tcatgttttc
    36001 tacgcaaatc gatcaatttc ttgtacatat aaaaaacaga atcttgatct gctaacgctt
    36061 cttctgcatt gatttcttta taatttggat tgactcgaag ccatggaatt ccagtagtaa
    36121 acccagcatg ttcacgggcg ttccattgca taggcgtcct agcattgtct cttcctttcg
    36181 cattgattga ttccaagatt tcctcttttg taaatccaga agaaatcctt tcgttataca
    36241 tattgattgt ctcaatatct tttgcttccg cgatatcatc gattgggcaa ttcgtcatgc
    36301 ctaactcttc tccttgatag atataagggg tgcctttcat catatggagc aagatagcaa
    36361 acatcttggc actttctttt cgatacacat cgtcatttcc ccaacgagaa acaattcgcg
    36421 gaagatcgtg attattccaa aataaactat tccatccttc atcacctaaa gatgtttgcc
    36481 atttggataa tacttttttc agttcagaga tactcaaggg tttcaaatcc catttgtctt
    36541 tgccttcttg ctgatctaat cctatgtgtt cgaattggaa gaccatggat aattcatttc
    36601 ttttaggatt ggaataaagt ttagcgattt cggtagttgc tccccatgtt tcgccaacag
    36661 tcatcagatt tttgtcacca aaagttgctt gattcatttc ctgaagatac tcatgcagtt
    36721 taggtccatt acttgtgatt tctttatccg gttctttgcc aatcatatcg atcacgtcca
    36781 tacgaaagcc cccaatacct ttatcgatcc agaaattcat catttgataa atctcttggc
    36841 gaacctgttt atttttccaa ttcaaatcag gttgacgctt gctatacaag tgcagaaaat
    36901 attgccccgt tttttcatcc aactgccaag cagaaccact gaatattgag cgcaatctat
    36961 taggctcttt tccattgacc ggatcacgcc aaatataata gtcccgataa ggattttctt
    37021 tatttttacg ggcctcaatg aaccacggat gttcatcaga agtatgattc accaccagat
    37081 ccatgatgat acggatattt cttttctttg cttcttcaat cagttcttcc atttcttcca
    37141 tcgtaccgaa ttctgccatg attgcttgat aattactgat atcataccca ttgtcatcat
    37201 tcggagactc gtatactgga gaaagccaaa ttgcatcaat acctaaatac gccagataat
    37261 ccaacctgga aataatcccc cgcaaatctc caataccatc ttgatttgaa tcttgaaaac
    37321 ttcttggata gatttgataa acaactgaat ggtgccacca tttttgttcc atgattttcc
    37381 tcctataaat ggatgtttct attttttgct taatcattta ttttagtctc tctcactaaa
    37441 aattgtaacg ctttcaaata gctattgcac cttgttatcg gtaacagaat aaaacaaaaa
    37501 aacagctgag gaaattccct caactgtttt ttgaaaggat tgatttatgc agggaagaat
    37561 gctgatttca caacttcttt ataaacggtg ttcactcagc tgtcccttgg tattagctca
    37621 aaaaacggca aaatatgagg aactattttt tcgttttttt ctctaatact caggccagaa
    37681 cactgatttc acaacctctt tataaacggt gttcactcag ctgtccctcg gtattagctc
    37741 aaaaaacggc aaaatataag aaactatttt ttcgtttttt tctctaatac tcaggccaga
    37801 acgctgattt cacaacctct ttatttcaca ttcaatatcc acatgaatcc cacaaataca
    37861 aagaacagac catacatgat tggagatact tcttttcctc ttttagctgc taacatggta
    37921 attggataga agataaaacc taatgcaatc ccatctgaaa tactataagt caaaggcatg
    37981 ccgatcaaga tcaagaatga agggatcgca atctctaagt ttccccattc gatttgtcgc
    38041 aaagattgtg ccatcaatac tcctaccata atcaatgctg gtgcagttac ttgtgaagta
    38101 acaactgata gtaatggaga aaagaacaag cctaacaaga agaaaatccc tgtagtaata
    38161 gctgtgatac ctgaacgtcc tccaacggcg ataccagctg atgattctac aaatgcacca
    38221 acaggagaag taccaaataa ggaacccgca agcatagctg ttgaatcaga agctaaagct
    38281 ttccctacac gaggcatctt gttgtctttc atgaaacctg cttggtttgc taatccaact
    38341 aaagttcctg ctgtatcaaa gaaagtcaca agcaagaatg tcagcactac gacccacatt
    38401 tgaagagaat tgatgtcccc tacatgtttt aatgcaacta aaaatgttgg tttcaaactt
    38461 ggtgctgcgg caacaatttc agaaggcatt ggaatcaaac ctgtgaccag acctaaaatc
    38521 gttgtagctg ctatcccgat gaagatgcca cctggtacac gccgaacaag caagatagca
    38581 gttacgacta atccaaaaat cgtcaaccaa gtagaaccaa cgttgagtga tcctaatcct
    38641 actaatgtag attcattcga aacgatgatc ccaccttcgc tcagaccaag gaaagcgata
    38701 aatagaccga taccgccaga aatcgcatat ttcaaatctg ctgggatcgc atcaatgatc
    38761 atttcccgca gcttgaagat tgtgatcaaa ataaaaatca aagaagcaac gaatacgccg
    38821 gctaatgctg tttcccatgg aatccccatt cctaaacaaa cagaataagc aaaaaaagca
    38881 ttgatcccaa gagcaggtgc cgttccaatt ggataacggg ctaatacccc cattaaaaca
    38941 cagccgatcg cactggctaa tgcagtcgca gtaaacactg ctccttcatc cattccagaa
    39001 gcacctaata cagttggatt tacaaataag atataagcca ttgagataaa tgttgtaaat
    39061 cctgccagta cctcacgttt taccgtggtg ttcaattttt ctatttcgaa gtaggaaagt
    39121 attttttcct tcatcttcca tccccctaaa tccgaacatt ataaataaag tccgttaaaa
    39181 tttcgattta ttaataaaac aacgtgtttt atcatatact aaaacaaaac ataagtaaac
    39241 aaaaaattcg gattttaagc aaaatccgaa cgttccatct gattaaatcc ctttatctca
    39301 aagttcatga acattcactt tggattacaa gaatttcttt attgtgttaa taaagtttct
    39361 tcattctagt ctcaaaaatc aatagtgtga tttgatttta ccatttcgtt tctgttcttc
    39421 tttgatgaat tcttttgtat cgacaggatt cagattattt agtccgccaa aacctgtatc
    39481 gaccattttt ccccagcctt ttgtcttctt ttttcctgat ttgagattct ttgctcgaag
    39541 cttctcacta tgttttgctc gccgatactt caaataacct tctaatcttc ccattagatc
    39601 cactgttttt cctccttttt attcaatcag acgactcagc ttttcctata acaaaataat
    39661 tgccttcagg gtcagaaaaa ttgaaagtca agatgccatt gttatctgtg atttctcctg
    39721 gttcttccat gttctcgtag atagtttcaa attcatccgt aaaaaatact aaagaaggcg
    39781 gtggtcctaa tacctctggt gaaaacttct gcacaaatac ttttgggaat acgcctaatt
    39841 ctaattcttt tgagatgctg agtacaatac tcttaaattc ttctggcaat tcgattgttt
    39901 cagaaatttc tgcaccaatt ttttctacaa agaatcggca aatcatctct acgtcattga
    39961 cataaagcat cacacgtgtt ttttgtatca tcatttgacc cttctttcta tttgattttc
    40021 tttcctgtct ataaactgat tccattatat cacgaaagcg caaacacctt gtgtttgata
    40081 ctattcaaga aaaataaagc aatagtttct aggattgctt tcctccacaa acttttttta
    40141 caaaaaaaaa aaacggcaat gaatgccgct cttcttattt cgattctttt ataattctgc
    40201 ttctgattca tctttggtta gtgtgtgtct aacactagtt acgccaaatg tttgatcgat
    40261 tttttccttc aacgcttcga ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga
    40321 ttcaatgacc agcaccgctg cttgtgtttc ttccgtcagc attgttcttt gggcttcacg
    40381 ccagttgaga cagcgacaga tcgctccttg ttcatcatag tagatgattt cttccggaag
    40441 tgccggagca tcggaatctg cacctaaagg caggaatgat tcgccacctt gggcttttcc
    40501 taaatgcaga tttcctgcga tcttttcgat atcttctccg ccgcaaggta cgccaaaaga
    40561 caaggaaata ctattataga tatctacaag cggattgatt gggaaaaatt cccgttcttg
    40621 atttgcacgt ttcagtaaag cttcaataga agaacgtgca ccttttttag tcttgaatgt
    40681 ggtaaatgcc tgacgccact gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag
    40741 gtaatttctt gcttcttttg ctgcctcatt caataattga tgatacgttt cttcactttc
    40801 ttttgttacg tggttatcga taccatttac ggataagatt tcgatggttg cttcaggaaa
    40861 tacttcccag aaattggtat ccactattac ttttttcatc tgttcacacc tcttgattag
    40921 aataattgct tgatgaatag atagtctgtt tggggatcat ctcccatata aaaaacatgt
    40981 tcgctgtatt tgataaatcc ttttttacga taaaaagcga gcgcaacatc gttgtgttcc
    41041 caaacaccga gccaaatggc tgttttttgc ttttcttttg ctttttcaat agctgtatct
    41101 agtaaaaatg taccgatacc ttgatgttta aatttttttc gtacatagat tcgctcgatt
    41161 tctaaaaaat gatcagccat ttcctcagat tgactcgttc ctgtatttag tttcaggtaa
    41221 cccgcaggct cttgatcgat actcactagg aaaaaatcgg tttctttatc ttttagctct
    41281 tctgtaagtt tttctagtgc ataagcttgt gtcaagtaat cagctaaatg ctccggtgaa
    41341 ttatcttttc caaatgtatc tgtaaatgtt tcgatactaa tttgttgtag taagtctaca
    41401 tctgaaggcg tcatttttcg aatttcgtat ttattcacgg cttcttcctt tcaatagatc
    41461 cttttattgc cttttttgac aaaattccaa tcttcgctaa cattcttgca tactttattt
    41521 aaaagatatt ccaattgttt tgcttcttcg tcagaaagac catttaatgc gactgcattg
    41581 gagtagttgt tttctcgaat gatgaaagga tagatttctg ctcctttttt agtcggatag
    41641 agtttcttga tttttttatt ttctttatct tccaaacgtt cgatcatccc attggattct
    41701 aatttcttga ttgctcgggc agtcgttgta cgatctactt tgatcatttc tgctagtttt
    41761 tcttggatga ttccaggatt ttcgcaaata cgtactaaat aaagatactg tcctcttgtt
    41821 aaatcgactt ctttgaattc gatattgcta atagaatcta gcgctcgggc aatcattccg
    41881 atgtctcgaa ggatttcagc catttcatcc cacctttatt ttgtttgtaa gactagttta
    41941 cctctttttg ttgcaaatgc aactattttc aataaaaaaa tcaagttctt ttttaataac
    42001 tagtaaagta tattcttttc ttcttttacg aaaaagctca atcacaatag atctggtttt
    42061 tgttttctaa aataagtaac tgtttcttcg ttgttcgctt ttctgatagc tttggcaact
    42121 cttctatact aaaaaatccc atgttggaag tttcattatt ctcaataaac ttggcctctt
    42181 caccgtggat cgcacaagca aaaacaagtt tataatactg aaaaagctgt ggaatatctt
    42241 tttgtttatt cgtatcaaaa actgctcgca actgagtagt ttctactgtt aatcctgttt
    42301 cttcatagac ttctttacgc acattttctt caggagacaa tcctatttca gcaaatccac
    42361 caggaagtga ccattctttc gtccgtttat cttcgattag taggaccttg ccatcttttt
    42421 tgatataggc acgaacatct acttttggtg ttggatgtcc ttctccaatc gttgtgagcg
    42481 cttttatttc ttcgaaagtt tcttttccta tttcactgat cagttccaat gaaatcgtcc
    42541 ggatctcctc aaaacgctct ttatcaaata catcttttcc ataatataga ccagcttgag
    42601 aaagtgcgag tagtcttttg tattgagcca acttcttgtt cattctcttc ccttcttttt
    42661 tagcaaatgt atgactatgt aatcaagtat agaagactgg tttaattacg gcaaataaat
    42721 ataatgaaat ctgcaataaa taaaagaatg ttaaaaaact cgagaacaaa agacattcga
    42781 cttctctggg gctgtgaata aatggcgttc aaaagcttgc atctgcggta ataagcccca
    42841 ccaaacgaaa aatatgacga gctattcttg tctggctgtt cttattaatt gaggcaggac
    42901 agctttttca caacctcccc actaaacggt gttctcctac caactcctcg gtcacaagtc
    42961 acaatattca tcaaacatga gaagctgttt ttttgaaatt gctccttgtg actcggagct
    43021 gaacgacagg ctcacgacct ctcggtaaac agcgttcaaa agccaatttt tcgatattaa
    43081 gccatttctt tgttacttat tgtcattgat ttctttttct aatttatcca atagtggagt
    43141 gtaatccact tgagtatagt ctgttacatc aaattcatat agtgttccta actggaattt
    43201 gtcatatttt cggtcggcga tgatcttttt gaatgcttct ttcgtaatat gattggtccc
    43261 tttcgttcga tcttccaata cgtcaccata gtgataatga tccatgatat agctcaaatg
    43321 acgatctgat tcacgatcac gtttgtaccg acgttgacat aacacttcaa agtctgctgt
    43381 taggcgaatc gtgatcacct gatatccgta tctatctgct aattcttgga gtcgtgcttt
    43441 ttgcttatca ctaaaaggat actcggacac aatcactttc tttcctgctt ccatatacaa
    43501 atcaagtgct ttgtaataga aaggataaac tttcgtcctt tcttgttccg cccgttcttc
    43561 caaattgtta aacccgatgg attctgcata gtattccttg atttcatcga gcgtgatggc
    43621 atacatatct ggaaatcgtt cccgtagttt attcataaga tatgtttttc ctgtaccagg
    43681 actaccagcc agaagaatga aatacttttt catctcattt atttcctaag ttaattggac
    43741 tttccaagtt cataaacagt ccaatcttat gcttgacaat ttcttttact gcttcatttg
    43801 ctggaatcac attatggcga agagacttgt tgatttctgt ttcactaaag gtttctttag
    43861 ctactttcgt ccagttgact aaaagctccg ttccaacatt gaatttgcgg atcccgttat
    43921 tgatcaattc aggataatct tcttctttca cacctgttcc tccatggata actaggggga
    43981 catcaactac tgcatcgatt tctttcagca agggaatgtt tacttctgtt tttgatttga
    44041 attgtccatg attcgtaccg atagcaatag ctaatgcatc cacgcctgta gcttctacaa
    44101 atttcactgc gtcttccggt cttgtatata ccttgtcatt ttcagcgaca tggattcctt
    44161 cttctgtacc gccgattgtt ccaagttctc cttctacgga aacaccgtgc gcatgggcat
    44221 attctactac tgcttttgtc ttcaggatat tttctttgaa aggcagatgg gacccatcat
    44281 acatgacaga agtatatccg ctgtcgattg cttctttgat atcatcaaag tctcgggcgt
    44341 ggtctaagtg caagactgca tcaaccattt cttcttctgc aatagctgtc attacattga
    44401 ctaacacatg catgccgata tatttagcgg tatcgacact tgtttgaata atgatcggcg
    44461 aacccatttc ttttgctgcg cctagcatag caggaagcat ttctaggtta tgcgtattaa
    44521 aagctccaac tgtaaaattc aactcttctg ctgtttttgt tacttctttc aatgttgtat
    44581 acatctttta ttgccacctt atctttttat ttgtttcctt tgatcgttac ttgttttgca
    44641 atttgtcgca tctcatccat tttgcccatg tagtaatcga tcccttgatc atcaccagca
    44701 cgagcagcat ctgcacgctt ctggttatag aggctcatca gttttttata cccatctccg
    44761 atcgtctgtt tattttccaa actcttttct aaatattgga tggcttgatc gttcacatac
    44821 aattcagaaa atttaagtcc aacttgttca taaagtttcg ctaatttatg cttatcttgg
    44881 gtagtggaaa tctcttgttt caattgtgta atctctgatt gaatcttttc ggtcgcttct
    44941 ggcgataggg ctttttcttc ttttgccaga acttttttcg gttctttttc ttttttctta
    45001 aaaaatccaa acatcatttt ccctactttc aattaaaatg ctttcaaaac tgggcttaag
    45061 atccaggcat ataagagtgc tgcggctact gtatctacat cggttgctgt agcattgatg
    45121 aatcccatcg aattaaagat cgttactagc aaagcgggca ataatgtaat aaagaaacca
    45181 tgagcaatac cgccaatgat cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct
    45241 gctgtccctc cggcaaagaa attggttagc ataccaggta aaatcatcgc taagccgaac
    45301 atgggcaaag taaacatggc aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg
    45361 acagcatttg ggctataagg aaagaaaact ggacaatcta aggcagggat cgcatcagga
    45421 actaatttca tagctatccc tcgaaatgcc ggcacgattt cacctaacag tagacgtaca
    45481 ccagctagca atacatacac acccactacg aattggatgg cttgtaaaaa ggcaaacatg
    45541 atatagtttt gatctcctga taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg
    45601 atgatatata gcgggaccat cacgaccatg acagacaaat aagtatcttg cagaaattcg
    45661 aaatgtgtcg gcaacttgat atcttcgatc gactttttat tttcgccttt ctcaccaaaa
    45721 agcttagcaa caccggcttc aaataagtac ccgatcgtac aaaaatgacc taaagcaata
    45781 tcatttgatc cagtgatttt acgaatgatc ggttgcgcta ctgccggcat agcaatggcg
    45841 aaacaagctc cgataaaacc accgaccaag atcaatacga tccctcgcag accggcaaaa
    45901 tagccaaaga ctgtcgtcat cgttgccatc cataaaattg cttgaccagt taaaaaaatg
    45961 tacttccatt ttgtgaaacg agccaaaata atattgaata caaaaatggc taagaacgtc
    46021 agtgcaatat ctctacctaa acccaaatca ttcattgctt gaccattgat agattcaata
    46081 gaagggatga tcccttgcat atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa
    46141 cttccaacga tgatgctcga accggcactc aacacttgaa atcctaggag tgtctttaac
    46201 gtgccagaga tgacttgtcc tgctgatttc ttctgcagta gtagacccaa catagcaatc
    46261 aatgcaatgg tgatcgatgc ctgagtcaaa atattttcga tgataaaatt gataacgccc
    46321 atgtttttac cctcttttct ttttgtattt agataactcc cttatcttgc agaactgggg
    46381 tgattttttc ttcaatttca ttttttgaaa caatattttt caaatagatc atcgccgttt
    46441 taggatcaac tgcgaacttt tcgaactgcg aacgaaaatt ttctgctgtg atgatcacat
    46501 ctggtttcat ggaaactgcg gaagaaatat cacaatggtc cagtttggct tctactccta
    46561 atttgttcaa aacatcctca gcagacatct gtgctgcaaa actacttcct aaacctgcac
    46621 cgcatacgaa taaaatgttc aatttgttca tctttgttct cccccgtatc atttattatt
    46681 tctcaactac tgaaaaaagc agttgtttga aatcttcgat atttgtgcat tcaatcaatc
    46741 gatccaactt ctcttcgttg ttgatcaatt caattagttc tttcatgatg ttcagatgcg
    46801 agtaggagtc tactgctgcc aaacaaaaaa taatctttac tggatccatt tcaggattac
    46861 cgaaatcgat tggttgctca atcgtagcta cgctgactcc taatttattg accccatctt
    46921 ctggtcgggc atgggctagt gccatgtgtt tgccaatcac gatatacggt ccgtaatcat
    46981 tcactgcacg aatcatcgct tcgacatagc gctcttcgac aatgttttct ttaaccaatg
    47041 gtctagcagc tagttcaatc gattcctccc aagtagtggc tttttctttg atcatgatat
    47101 tgctgtccgt caaaatatct tttaacatag gctggatctc ccttttgttg atttctaaat
    47161 tgtttgtttc gaataatttt tctaacttgc catagatgat tcgatctact cggccaccac
    47221 tttctttcat gacatctatt atttgagtga ataactccgt cgattcatga ctgttaggct
    47281 ctaaccgctg tagttcttca tttcttttca aaaagtcttc tataaccaag atattcgtct
    47341 cagttaaaat cggatcgaca actaaaagcg gcttatttcg ataattcaat tgaaaggtgc
    47401 tgaaaaccag attgacatcc aatttatcta ctaattcgat ttcttttgaa cttaaaactg
    47461 cagtaatctc gatctgagga aacttttctt tcagattctc tgctaacaga tttccagtag
    47521 ccatcccatg gttacagaca acgactgatt tataaacata attcaagtct cgcttgatta
    47581 cactggcgat cgtcgaaaag tggatgacaa gaaaagctat ctcgtcctcc agtatcttgc
    47641 caccaattac ttcttcgatc gtaggaacaa aatgagtgac tgcgcggtag attgggaaat
    47701 aatttgttcg gatgttttct ttcaatggat tcatgacttg gatatgattc ttgattctta
    47761 caatcagtgc agccatgtgc ttatagagat tttcgaataa agcttcttcc tttagatgga
    47821 aaggtacgtg tgtctcgttc tctacaaatt ggatcaattg gatcgttagt aattgagctt
    47881 gtacccactc gattgaattg gagatatctc tagcattgaa cgtctcaata gtgaaacgaa
    47941 gataatttct ttctacttgc gggacattgt ccaattgaaa ttcctcgatc atgtggtcaa
    48001 taaatgcaga aaagctactg tcttttattt ctgtattttt ccaattaatg gcagagataa
    48061 attcatgttt cctgattcgc tggatccaga tcaatgtaaa aagcaggatt tgttttttat
    48121 aaatatcatc ttcttgtttc aacaacttat tggaatagat ctcctgtatt tttttgattt
    48181 ccaccaaagg aaaataatgg agaaagattt tttgagccgg tgttaattgt gcatcttgat
    48241 tcataaagaa gtcggtcatt cccagattct gattgataaa atcatagatc atcgtacgga
    48301 tagatcgttc tgaaccttta taaaccagtc cttgctttcc ataactgacg atctctatcc
    48361 cataactgac cagtgttgct cgtaaccgtc tcatatcttc atctaatgta cttttagaga
    48421 tttgaaattc ttcttctttt ttgtttagat ggatcgggat tcggctgtaa gcaacggcta
    48481 aaactagatc aaaaatacgt tcgcgccgat tcaaaaagcc gtctttcaca tcttttattt
    48541 cagtttctaa tcgagcttgc ttttcctttt ccagcttcaa caaaaaaact ttgttgcgaa
    48601 ttgtacggat ctccggaagc tttttactgc tcaaccagat attcgcttca agaacttcct
    48661 gacggatcgt ccgctgactg acattgaact cacttgctag atgttttgct tttactggat
    48721 attgggctgt catgattctg tgaagtattt caattgttct tgttttcata gaaatgatct
    48781 ccttctgatt tcaagtatat ggttccgctt acataataaa aagtagatat ttctgccact
    48841 tgaagtggaa agaaagtaag caaagcaaac aaaaaataag ccatatgctt tttcatctcc
    48901 tcaacgaaaa agacatacgc ctattattac aaaaattttg cccatctata caaaaaagag
    48961 gttgtgaaaa agctgtcctg catcaagtaa taagaacaac taaactaaaa tagtgcttcc
    49021 tatttttcgt ttggttgttc ttattatcgc agatgcaagc ttttaaacac catttagtgg
    49081 agaggttgtg acagagatgt tcagccttga gcgataagaa gcgattttga aaaatcgctt
    49141 cccatatttt acaaaatcgt gccttattgc cgataggcta tctctggcac actgtttatt
    49201 cagaaaaagg agctgcgaca aggcttttgt cacaactccg ttcataaata tttcctgtca
    49261 atccagcata atcggctgaa taaaatcggc ttgccatctt ttttctttct cagaaaaatc
    49321 aaaataccat atctgagaaa aatactgtcg ttgtgtctta cttcctgaga tcaatcgccc
    49381 atcttttcga tcgactgaat aatcacagca tctgaaatca attcggatgg caaaactatt
    49441 ttcgtggatc tgtcgataat ttcctacttc ctgaatagtt atttttttag tatgatttct
    49501 tacaccgtct gctttgtttt tttgcagaac tttctgatgt tcggcaaaca gcttgtctgt
    49561 ataacagctt ctggcttttt caggagtttc ttcttcccaa gcggattgga tcttcatgaa
    49621 tatcttttgg atttccagaa actgtgcttt cttttcttta gccgttcctg acatgcggga
    49681 caatatctcc gctttcagcc ttttcttttg tcttttattc ttgacgatag aaaaagcaga
    49741 aacccctcct actgacaaaa cagctaatgc aagaaatccc gtttgctcat aactagaagg
    49801 acctgtacct ggaccataat acccataaga atctctgccg taagaacgac tgccaaaata
    49861 actgccccca tatgaacgac caccaaatga acctctgccc gaacttcgac cgctagatgt
    49921 acctccacca tgaccacccg cgacagcgaa tccttgggtc ggcagagcaa acaaacatag
    49981 gcccactaac aaaatgattg ctaaataatt gatttttttc attgtcttcc tcgttatctt
    50041 ttattacaaa aaactatttt tatgatagcc ttgctaaaag gaagagtcaa taaccagatg
    50101 cataaactgt ttttttagca attttgtctt atttagtcat tgagtaacat cggacaaatg
    50161 tatgtgtcgg cagtccataa ttcctctatc ttcagcatga acaagcttgc agacaagaga
    50221 tctgcgcttc ctccaggaga gatttggttt tcctgacaaa agtgcaacag tgctgtaaat
    50281 ggacgatttg gtaaagcact ttgactagca gcatgagcaa gtttttgcat ttccactaac
    50341 gttttctgat cactgcgatg caatacgcat gtgtcatcta gcgaactcat caaaactaac
    50401 aacatatgca tttgtttctg tacatatgaa tcattatcta gctccttagc tgcttggatt
    50461 gcaagcctga tatgcggata ccctaatatc gcttcttcat atgcaccatt tacgccatat
    50521 ttttgcttca ccctatgacc atgagtggtt tttttatgac gatattttgt atcaggaaaa
    50581 gcagcaagct tgctgacatc atcaagtatt ttcgataaag aaaaaggctg ttccatactg
    50641 attttttgtg caatgacact gatcaatagc cccattgccc aaatcgctcc tttatgggta
    50701 ttgatgccat ttgtagcaat cagcattgcc ttttccgctt ttcgcccaat ctcagcaatt
    50761 ctttctctta actcctgatc aatagcatga tgccatgcta tttgagccat ttcttcaaaa
    50821 aaaggagtta gcgtcttagc agacttaaga aataaagcat agtccatatc ggaatgtgca
    50881 cccgcatcca gtgcatccac taatccaggt tttggactta atgttacttc tgcgatcaat
    50941 gcttcctcta ccaacgcagc gagttgctga gtcactgctt tttttcgttc gtcatttatc
    51001 atgacactca ctccttcttc ctactattac ttgctgaaac cttttttgat agctacccaa
    51061 atgatatgaa gtaaccagct taaagcaaat gcacaacttg ccgtcattag ataagctaca
    51121 agaatcatga ctcctgaagg taactgcagc caactatctt taaacaataa taacagtact
    51181 tggaaaacaa atacattggc taaatacgta cgataagcat aaacagacaa ccatttgaac
    51241 aatggcaatt ttggcgaatc gtttacaatc attttggaag cgcctatgaa gacagcaaag
    51301 atgatgacta aactgtataa actttggatc gtattcaagt aaggtgcatt cgcaaagctc
    51361 aagtctcctg gatgtgcgag caatgaacgg acagaaaaga acgcgataac taaggcaaca
    51421 ggtaagaaaa gataacgtac tctgtccaaa tattgataga ctttttcgtg ataaacaaat
    51481 gcggctaccc cataaatcat gaaactagca acaaaccgat ctaataagta ccagctgtca
    51541 tgataaggcc cagtcaatac ttgtgtttga tagaacagat accatgcgac atataaaatc
    51601 gttgaaccaa tcaatactgg ccatacatat tttttattag caaatacttt gtaaccgagc
    51661 caaacaaaaa acggcatgaa caattggatc tgcagcatca taacggtata ccataaatga
    51721 ggcgctccat cacctgtgac atatttcagt aaaaagcttg cagtatccgt atacggagtt
    51781 tgttgttgga ctgctggaaa tagccaaaga taagccgtcg tccatagaag atacggaacg
    51841 actagttcaa aaaacttttc tttcaaaaat tcccaataag acaaatgctg acttgttttt
    51901 accatattat aaacaattgc aaaaataaac attggtgctg tgtatttaac gatgacgtaa
    51961 aaggtaccaa gaaaagctgt atttgttgcg ctgtggctgt ttgccaaagc aaagctcaat
    52021 atcgtttgca tcataacagc cgttacagct gatactttct ggaagtcact tacgttgatt
    52081 ttgatttttt tgttttcaaa catctctttc tctctctttt tctttattac caaagttctt
    52141 tggttaattt aggtccttcg actgttttca atagaatttt tttctctggc tctctcaaat
    52201 actcttttag tgaaaaaccg ccttgccgag tcactacttg ggtatcgaat tgctcaaaaa
    52261 aagcaggatg cgattgaatt acctgaattg gcaattcaat cggtgaattt gcgtacagca
    52321 gcaaatcaaa atcactggtt tcttttactg ccggaatccc agtcgataat tcaaatccta
    52381 agctgccgcc gattccccat ttacatcctg ataatatgtt tctagcagca tgaaattgct
    52441 gataaacagg ataatccatg attttctctt gtctatacga ttcttgttcg atgatgtccc
    52501 acggatgaat gacttgtttg acggcatgtt ttgagatttc tgttgaccat ctctgattgc
    52561 gggcaggtcc acgtaaacca atcggtagta tttctgcgtg atccgtcgac gcacgacgaa
    52621 ctgttccgaa catcttttca gttagaacga cccattcagg caaggtgaac ccttctattt
    52681 catgtttatc aaaaaggaca atatcatgtg cttgaatcat tttttatgca tcccattctt
    52741 cgtccatttt ggcacggact ttatttgttt cgacacgtcc gaatgttttg gctgtttcgt
    52801 ttgtgtatcg atttgtcagc atcgtgtcat ttgtattgcg gacatcttga atggcttcat
    52861 tgattgcttc gatgactttg ctgacatttt gttctgaagc agcttggtcg gtgatatctt
    52921 ctaggaaatg atagatcgct cctagttttg tataattatt gatatcgtaa gcgatagctg
    52981 gaactttttc agcagctgct tcgatttcag caaccgaacg ttttgtcaca cgtgcagcac
    53041 tttctttaga catcgcttgg acggtaattt ctttattgtt taaagcaatc aagcggcttg
    53101 attgtaatcc atgtgctaaa aatgcacctg aaatagcatt tcctgcaatc aatccaataa
    53161 ctggatgtcc agcttgacgg agttttgcat aagcagcagc actattagcc aaagccacat
    53221 ggataccgat caattcttcg ttatagccgt atgcttggct tggtacatcg atcacaacga
    53281 tgattggccg tttcactttt ttgtctttat cttcttcgta aacgtgatcc aaaatttgtc
    53341 ccatgcggaa accttcaact aaaccaactt caccgtttcg tacacggtac atcgggtttt
    53401 taggatcagg tacgattgat gtcacgatgg cttcttctat gaatttactt gatttaccat
    53461 aatacacagt actaatagaa gaagttggat ttttcatgcc agttagtgcc gcaaaccaat
    53521 cgtaaccaac acttgctgca gctggttcat ttccttctgc tacttctggt aagtcaattg
    53581 attttgcttg atggttagca attgcttcat tgtaatcgtc aatggccatt ggttttgaaa
    53641 gatctaaaga atcaagtaat gataaataaa atgctgagct ttcactgcga tgcgtgtctt
    53701 ttcgttcgtc cattgctgcg atgacagcag ctttgattgc ttctacacta tcagtcacta
    53761 attcatcaat gatttttgat tctacccgtt gacgagatcc taatgtattc caaatcaagt
    53821 ctttgtcact agaatcaaat tctcgaacgc ctgcttcttg ttcaataact tctggtccat
    53881 tcaaaccgac tctcgctttt tttgttgcga ttaaataact cattaatgct gaagtaatcg
    53941 acataccgcc aaatgatcct acacgtcctg gaatcaaacc gatgactggt acatattgtt
    54001 ttagagcaat gattacattt ccgatttctg aaatagataa cagtccgtag tttgcttctt
    54061 gcaaacgaac accacctgta tctagtacaa tgactggaaa gatctcattt ccttttttat
    54121 tttcttctaa cacatgttca agtgcagcaa cgatttttgc accggaaact tcaccgatac
    54181 cgccaccttg gaattttcct tccatagcga ttactactgc ttgtttgcca ttgatcgtgc
    54241 cacggctgac aacgatcccg tcatcacttt cagggacgat tccttgtgct ggtaaacttg
    54301 gcgcgatgat ttgatcgaat ggatcaatca attcacgtcc attgtcaagt agagcgttca
    54361 ctcgttctct ggcgtttaat tctacaaaac tatttttcat ctgaaagttc ctccattgct
    54421 tgcgtcaatc gtaggtacac aactcctggt gtagcaccga agtcattgat cacgatatct
    54481 gctgaaattg gataacgatc aaaaaatctc gttaatacat tgttccatac ttcgccgaag
    54541 ccatcgcttc ctgtacaaat ttttacagta gattctgttt tttctgttgg aaacatcaag
    54601 atttccaaat ctcctgaacc gaccactcct acatggattt tcttattgat cggctgtttc
    54661 gttggatatt tataagctaa ttgttccatg gatctctccc ctttttgtaa aacagagcat
    54721 acacgttctg cttattcgtc tttcttcttc catctgggta aatggcgaca aaaggttttt
    54781 ctcgccaatt ttatgaccaa gttctgaatt tcattggtgg ttcatacagg ccatctgacc
    54841 attcgatcaa atcatcgatg gtttgtgcag ctaataatga acgtttcgct tcactacgtt
    54901 tgatgcctag atcttctggt aatttcacga tgccggctgc acgcaagcta tctagtgatt
    54961 ctttcgtgct agtcattcca agaggtgtaa caccgccaat tgctgcaatt gctgcttgtc
    55021 gttcttcttt tgtttttgct ttgtacaaat aagcaattcc ttcttctgtc acgatatgtg
    55081 tagtatcttc gctataaatc atcactggtg cattggctaa gcctgcttgt ttttgaacag
    55141 ctagtgcatc taattcttcg acaaagactg gttttttatt tgaaccatat gtttcaacca
    55201 tttgaacaac taatttttgg cctttcccta atggatcatc attgtcgcga agtgattgcc
    55261 atgctggtgt agaatgacga cgaccgccag gattatgtcc catgttaggc gcaccgccaa
    55321 aaccagataa acgtcctttt gttacggtag aagagtttcc ttcgtaatct aattgcaagg
    55381 ttgaaccgat aaacatatcg atggcatatt gtcctgcagc ttgtgataat gttcggttac
    55441 tgcgcatcgt gccgtcttta ccgacaaaaa agacatctgg gcgagcagca atatattttt
    55501 ccataccaac ttcgccacca aaactatgga tgctttcaac ccaacctgat tcgatggctg
    55561 gaatcatcgt tggatgtggg ttcaataccc agttcttcgc gattttccct tttaacccta
    55621 gtgattcacc ataagttggc agtaacaatt cgatagctgc tgtattgaat ccaataccgt
    55681 gattcaaaga ttgtacgccg tgttctgcgt agattccttt gattgccatc attgccatta
    55741 gaatttgcaa ttctgtgatg ttttgaggat ctcgtgtgaa taaagcttct aattgataag
    55801 gtttatctgc tttcacgaca acgtctaccc agtcaccagg gatatctaca cgtggtaaag
    55861 aatcgacaac ttcgttgact tgaacgatca cgataccatc tttgaaagct gcagcttcga
    55921 tgattgttgg tgtttcttcc gtattgaaac ctgtgtacaa gttgccgtct ttatctgctt
    55981 tatctgcagc tactaaaaca acatttggaa tcaaatcgat gaataagcgt ccataaagtt
    56041 ccaaataagt atggatatca cctaatttca ttgtgccgtc ttcaatcatt tgggcgacac
    56101 gcaagctttg tgcaccagca taagagaaat cgatcttgct tgcgattcct ttttcaaaaa
    56161 tattcaaatg ttctggtcgt gagatactag acatgatcaa atgcagatca ttcacttttt
    56221 ctgcatctac tttttcaaga ctttctgaaa gaaaacttgc ttgtttttga ttgtcgcctt
    56281 ctaatacaac tttgtcgccg ggagcaatca gtttttccat tacttctacg attttttcag
    56341 tttctacata cttgccattg agcaagttgc tgattttttc caatcgctgc gctttttttt
    56401 cgcgtttaga agcccagttg cggtttggtt tttccataat tagtcatctc catttttgat
    56461 atagaaacgt ggcgtcatca ataccatgtt ggtctagatt gatcgtacgg atccctttat
    56521 ctacaccaaa ttttgtatga atcagctttg tcaaagtatt gccgggggga aattcaatcg
    56581 tcagatccat tcctaattct tttgccaagt ccatcatttg gttccactgc acagggaaag
    56641 ctaggttttc caacaaatct ttacgaatct cttctgcgtc ctgagtggct cttccatccg
    56701 tattttttat atacaaacat ctagcatcag atagatggac agactgcata tacggcttga
    56761 atttcttgac cgtatcattc atcaagagac agtgagatgg cacagggact ttcagcagct
    56821 tcgctgtttg cgcttggtgc cgtttggctt ctgccaacgt aacggataaa ccttcgatac
    56881 tcccagataa cgtatgttgc atcggacaat tttcatttga cagataaact ggatggtcag
    56941 gatcaaatgt ttttgctaca atttcctcag cttcgcttct cgtcacacct actactacgc
    57001 ccattgcata accgcttgga taagcttctt tcattaagac tgcgcggtga tagactagtt
    57061 ccgctgcatt ctcaaatctc aaagatcgac aagcaacagc tgcacaaaaa gcaccaatcg
    57121 aatgtcctgc tgcaatatca ggctgaatac cgtttttttc catttcatct aaataaaata
    57181 ctgcttttgt cagtaatgct agctggataa aaaccgtatc ttgatagttt tcttctttat
    57241 caactaaccg ataccccgtc acattttcta aacgttccat gtacatttca gggatatccg
    57301 ctaacatccc taacttctga ctgccttgtc caggaaataa gaaacctgta ctcatgagcc
    57361 tcgcctcctc agcacttact ttttccactg ctatcttagt caatttctgc tataataaaa
    57421 aatgccaaaa aaagaaaaaa ccataacaaa aaggtatgga tgggatggat aattatgaaa
    57481 ttgcttcatc tgcagtattt taaaaaagta ttcgaaacaa aaaatgttac ccaagcagcc
    57541 aaagaattat ttatttctca acctgccttg agccgtgcga tcaaacattt ggaaaacgaa
    57601 ttaggtgtcc ctttgtttta ccatagcggc agaaacattg agacgaccgt ttatgcagaa
    57661 gaattttatc catatgcagt caagacatta gacacattag aagaaggaat caaattattg
    57721 tcttcggtca atgagaagat cgttacttcc gtcatattgt atttagaggt agcctctgtt
    57781 tctattccga acttggtccg aatttttatg gaaaagcatc ctgatatcca attaacgatc
    57841 atgcaacatc atttaccaga tgatattacc gatcctgtac tgtacatcac ttcggagatg
    57901 aagcctggca tgaccaatat tcctatcatg aaagaacctg tttatgtcgc cattcctaag
    57961 aaacatcctc ttgctaaaaa agaacatctg caattagaag atatccaacg cacccccatc
    58021 ttgatgctgt caaagagcaa cgccttccgt catacactgg atgcagcact tgaagcgaaa
    58081 agtatcgaat tgacgatcag ttcgacgaca gatgatcctg cgacattacg ttctattgtc
    58141 aagcaaggat tagctgttag ctttttccca aaagtttctt ggtcttacga taaaaatgat
    58201 cctttcgtct tgcgggaaat caaagatttg cccttgacac gaacaattta tttatcttct
    58261 agcttcacag aagatcatcc tttgatcaaa atgattgcaa aaacgatgcg ggaattttat
    58321 ttaggaaaat aaaaaaaaaa gagctgggac aaaattcttg tcgcagctct tttttcgaac
    58381 aaacggtgtt ccagaagtaa tttcttagaa aataagccga aatttcccaa aaatttgaga
    58441 agcaattttc gtaaattcct tcttatttct tgaagctaaa catttctgtc acaacctctt
    58501 ctctattgtc ctagacattc attgcgttga tttaaaactt ccatcaatca aatctgctag
    58561 ttcatctagc ttttctattt tacgtagatc aatagagatc caatgttctt tattcatgtg
    58621 ataggctgga aaatacccct cttttcctcg caaaattccg ccaagttcag gatcgacctt
    58681 caaatcaaga atattgatga tcctatggtc acttgcatca tcatagattt tttcttctgg
    58741 aacagacatc aggaccccaa accatttttt gcttttttga tgtctgaata cgcaataatc
    58801 aggaaatttt ttaaaaatat attctggaga caaattgtat gtttcattga tgtgatcgat
    58861 tagttctttt ttcgttagca tctgcttcca tctctttctt attgattcgc tttattcaaa
    58921 gataccacgt aagaatagaa aaatgaatac aagtagttgc taatcaaata cttcgatatt
    58981 tttctttttt atcaactctt aaatgttcaa tccgcctgtg ttggtcttgc taccatctac
    59041 tattaaaatg tttattattg tttgcccacc tattttttga ttttagtggt atcatttttc
    59101 aagttcatga tgaaattctt atcatcagta aatgctccag aaaaataaac aatgattgaa
    59161 aaatcagttg aaacttgacc accgtttatt ttctccgtat tcttagaata gaagggagtc
    59221 tgattgatgt attcactaat gaaaaaaatc attaccgaaa aagatattcg acgtcatgtt
    59281 tctttagtag aacaattgct gaatcataca aagataaccg taaatgaatt agctgaaatc
    59341 atagggacaa ccgaacgaac gattttttct gaccttcaat ctattcgctc tcagctgccc
    59401 gaaggctggg acatcatcag cgatcaagca ggaatcagtt tgcaaaacca gcaaaattta
    59461 ttaacaaatg acctttggga gatttttttc aaacaatcag tcagtgtcga actgctgaaa
    59521 aatctgcttt ttacaaaaaa agttgctgtc cctgattttc tagccgacca tgggctttct
    59581 tatgggacat tgaaaagaca tgtgactaaa atcaatcagc gtttagcaag ttacgacttg
    59641 cagatcgatt tgacgaaata tacagcttgt attcttggaa aagagcgtgc gattcgtact
    59701 ttttatcatc gattgctgat tcctttcact cataacaact attttttcga ggattattcg
    59761 atccatgaat cccattattt tcaattttta agaaacctca gtcaaactga actagcggtt
    59821 gaaacagaag agattttcgg aacatgttgg ttctttatca atacgatccg tataaaagcg
    59881 aattgccgat tagattcttc tatccatacc aacagcacac tctcctccct gtatgactct
    59941 gcattgaaaa aattgtattt aacggaaggt atttatctta aagacactga gctttctttt
    60001 gcttctttct gctttctgga aagttggaat tacaacaata actatggaca agaaaccgct
    60061 cgctgcctgc atcattctcc atttttagag gtcctcgaaa cgtttgtaga ggaactagct
    60121 tcagagctgt ctcttgatca actaaaaaaa acatctctga ctgataactt ggctctcctc
    60181 atattaaaat atcatgaatc gccgatattg attgaagcat tggaccgtca ataccatcgt
    60241 tttttagaaa catatgatca gcaatatgca cacttgtatc aacagaaaga gaatttactg
    60301 aacgatctaa aaaaagaaat agaaatcaat gaaccaaatt actttctcca actcttgtct
    60361 ctactgatcc aacaaacgat tttttctatc aaaccgaatt tgttcaatgt ctattttatt
    60421 ttccaaggag aaccttcatg gaaggcgttc ttgcagcagg aattaaaaga ttacttagga
    60481 aaacgtgtct gcttcttacc tatcgaacta accgatcttt ccactatttc ttttgaaaaa
    60541 acagatatct tgatttccaa tttccctcta gattctctag atatccctat tgtatacatt
    60601 tcttctattc caacgaaaaa tgagctcaat cgtttgactg aactcacttt caaaactttt
    60661 ttatagtaac ggctagcgac tgtattctct agctgttttt tcttttttgc gaaacagttt
    60721 gttcatgatc cctttttcta tttctacaac taagaataga agaatcacat ttgctccaat
    60781 caataactgt tgtgtcatcg ttaaacttac tgtaccgatc agctgctggc cgactggtag
    60841 aaaaataacc atcccttgca ataaagcaag gatacctaat gaaagaaaaa gcgctttgtt
    60901 ttgcaaaaga ccttggttca atgacggctt ggacaactct ctgcaattga tcatgtaaac
    60961 agcttgagct aagacgatat tttgcagaag aatggtttgc tgtaagcttt gcccttcaaa
    61021 ctgcatagcc aataaatagg caggaaccat gatcagtagg gagacataga tgatccgaaa
    61081 caaatgataa ccagatagga ttccttgctt tggatctctt ggtgggcgct tcatgatttc
    61141 tggatctggc gactcgaacc ctagtgcata agataaagtg attgtcgtta ccatattgac
    61201 ccataaaatc tgtactgggg acaaaggtag cgggcgattc agcataagtg cccaaatcaa
    61261 gatcaatcct tgagctaatg cagttggcaa gaagaaattg atggtttttt gcaaattatc
    61321 caaaatccgc cggccctctt ttacggcttt tgcaatcgta tggaaattat catctcctaa
    61381 aaccatatca gctgcctgtt tgcttacttc actacctttg attcccatag caattcctac
    61441 atcggatctt ttcaaagcag gtgcatcatt tacgccgtct ccggtcattc cgacgatttc
    61501 tccatttttc tgcaaagctt taacaattcg cagtttatgt tctggtgtcg ttcgagcaaa
    61561 gacatcgact attggcactt gctgaatcaa ctcttcatca gacataagat ccaaatcgat
    61621 cccttctaaa actttggcag tatgttttaa gccaatttgt tccgcaatcg cttttgctgt
    61681 gtctttgtga tcccctgtaa tcattttcac tgagatccct gcttgttggc attcctttac
    61741 agctttgact gcactttctt ttggcggatc gatcaatccg gcgatccctg caaaagttaa
    61801 ttgggttaaa gtctcatgcg tgagttcctg tttcgaatct acttttttat aagcaaatcc
    61861 taaaacacgc tgaccttttc tcgcaaattc tgcagctgtc tgactccatg cttcttgttc
    61921 tgcagaagaa agagttgatt tagcaaataa tacttctggt gcacctttga cgtaaatcac
    61981 cgctcctttt tcctccgcat gcatcgttgc catatatttg tattcagaac tgaacggaat
    62041 ctttgcttct atttttttca aaggtagatt ggctgtttct gtgtgtcgaa tcagcgcaac
    62101 ttctgtcgga ttgccactta aattttctgt tttctgttct tcttttgttt ttaattcctg
    62161 acagtttttc ataatggaca acacgcaggc ttcttctttt gcagctacat ccacaactgt
    62221 catttcattt tttgtcaatg ttcctgtctt atctgaacag atcactgtca tagaacctaa
    62281 tgtttctacc gaaggaagtc ctttgatgat cgccttttct tttgccattt catgtactcc
    62341 aacggataga atcatggtca aaacagctgg caatccttcg ggtaccattg acaccaccaa
    62401 agcaatcgta gccgaaagaa ggatatgcca atccatacca tatcgaaatg tcgtaaaaat
    62461 aactaagaat aggctcaaac aaagaatgcc ttgaaaaatt tgtttgttta atcggttgat
    62521 ttttttaatc agaggcgtcg tttgttgttc gaccgattgc aatgcttgat tgattttacc
    62581 gatttctgtc gtgtttcctg tttctacaac gacaccgact gcagaaccag attgtacaag
    62641 cgttcctgaa aaagccatat ttttctggtc gccgatcgat tcttcttctg tcaaaacggc
    62701 atgacttttt tcagatgctt ctgattctcc cgtcaaaatc gattcttcca ctaataaatc
    62761 atgtgcttcg attaatcgga tatctgctgg aacgacatct ccggcacgta agacaatgac
    62821 atctcctttt accagttctt tcgcagggat tttttcttgc atcccgtttg atagtactat
    62881 cgcttcttgt cccatcagct gcttcaaacc attcaatgat tcttccgctt ttctttcttg
    62941 ccagtaacta acgagtccat tgacgatcac tacgaataaa ataatacttc cttcaatata
    63001 ttcactagaa gcaaatttca aaaaagaggc aatgatcaac acgatcatca gcagatcagt
    63061 aaaatgcttt gctatttttt tccatcgttt tgtctgctgc cttgctttta tttcattata
    63121 tccttgatca gtcaggcgtt tctgtcgttc ttcttgcgac aagccttctg ttgatgtttg
    63181 atagtttttc aatacatctt ctaccgtcaa tctgtaccag ttcatcctat cccctcctgt
    63241 ttaattgata ctgttattat acgcagggaa gttttttcag gcgtatcgat tatttcaggg
    63301 gggtctgaaa taattcgttt tgcaaaatgt atctgggcct aactgatatt tctattgctt
    63361 attcttttat atacaaaatt taagaataca aaccagataa ccaataaaaa aggaataaag
    63421 cgtgtcgtgc tttcgaataa caagctgaaa gcaattgaag taaaaaacac caacaaagca
    63481 agatctttat atctttgtgt agtgttccgt ttcttttctt taataaattg aacatgtgca
    63541 cacaagatcg aagaccacac aaatagaaaa caactcgttg tcatgcctgc caaaagctga
    63601 aaaaacaatc gactatcagc tacaaaaaaa tgtagacaaa gaccggcaaa aaataacatt
    63661 cctgtaatta aaataccgtg taaaggtacg cctttcttag aaagcgcaca aaaccaatgg
    63721 ggcaaacttt tttcttttgc tgtttgatat aaaatcctgc tagtactgta gactgcacta
    63781 tttgctgagg acaaagaagc aaccaaaatc attagattga caattgctgc tccgccttgg
    63841 caatggatca cttgcagcat agtcactaaa ggactttctt ttaaatctaa acaatgccat
    63901 ggaacaacaa ataaaatcga caaaatcgtt cctatataga aaaagagaat acgccaaggc
    63961 agacagttga ttgctttttt caaattcttt tgcggatcac ttgtctcgcc aacggttaag
    64021 ccgatcattt caattccagc aaaactgaat aaaaccatag gaaatgagac taccattcca
    64081 gcaaagccaa acggtagtaa ttcatcgatc tgtccaaaat tttctttgat tccgatagac
    64141 tgggtatgaa aatctgagac actcaatcct atacccaata agataaaaag gaaaatcccc
    64201 aagatcttga tgattgagaa aaaagattcc aattcaccaa accacttgac tgcaacaagg
    64261 ttgactgcca ttaagatcat tagtgcgcta cctccgctta tcaaagctgg tacatgtaca
    64321 aaccagaaat ggatatacaa tcctattgct gttaattccg caagtccagc tccaagccag
    64381 caaagccaat aagtccaatt gacaaaaaat gcccactttt tgcctaaata atagtcaacg
    64441 aaatcactga tagagttaaa tgtactattt gctaaaaata aatcacccat ggcccgcatc
    64501 aaaccataaa tagccaaacc gcatatcaaa taaacgagaa taacagctgg ccctgctgat
    64561 gcaattgcct ttgctgaacc taaaaataat cctgttccaa ttgcgccacc taatgcgatt
    64621 aactggatat ttcgattagt tagagtccgt ttcattgccg gtttttccat caagttcacc
    64681 tttttctttt tcaagcagca tggcgacttc ctgttcccaa gaaggtttct ttattttttc
    64741 aatacagagt ggtactacaa acaaaatgac actgatggct gtgactgcaa ttagattgat
    64801 ccatgttcca gctttcatcg tcagtccgac agaaaatccg attgccgcaa ataaaacaat
    64861 aacgacagtc caaactccaa gattgctctt tttctctttt tttccaattc taaatggtcg
    64921 attcaacttt gcatgtgtat aacgcaatct caaaactgcg atccccatga tgacatagac
    64981 gatacagtag ataacagtag ttgaatttac gagcatcaaa aatgcttgat tgacgccagg
    65041 aatcagcaaa taaaaaagtg caaagattga gataatgaca gactgagtca aaataacgga
    65101 tttggatacg tcaaattgat tcgtcttcca aaaattgaat tttggtggat acgcaccttt
    65161 tctagcactt tctgtcactg tctttgctgg tcctgaggcc caagcagata attggactgc
    65221 tacaccaatg aagacaagta aactaaacag attcacaata agatgtggca aaccaagaat
    65281 ttggcaataa atcgaaatag attgcgcgat attattcaat tccatttgtc cttttggtac
    65341 gacatttgct acaactaaag cattcaaaat attgacgaaa aatataaaaa ttaacgcggc
    65401 aaatactccg cgaatatacg ttttaactgg attttccaat cgagtgatat acacagagga
    65461 catttcgata ccagtaaaaa taaacatgat cggcgcaaaa taaacaaatg aggatgcagt
    65521 cgtagtatct ggtattaatt tgtcccatga aaaactgcct aatgttccgg ttggtacgat
    65581 cccaactttg atccacgcag caaaccctaa taagagcatc atcgtcaaag gcacgtacaa
    65641 tcctaaccaa acaccgactt ttccgccgat ttttgccata tcaaatttca aattcaaaag
    65701 agtgatgatc caataaacga ctaaaatgac aactaatgta aatttgctgt tcaaaccaag
    65761 cggcacatta tcgataatat taccaaacaa cggagcgagt gctgaagcga ccatcaccat
    65821 cccaggaaac atttggaccc ataacagcca tgagacaacg aacccccatt tttttcctag
    65881 agcattcgat acccaaagtt ctggaccacc tttttgggga aacattccag caaattcacc
    65941 agagatcaat gagattggaa aagcgtacaa taatactgca accagcatgt aaaaaatcat
    66001 cgtccagctc gttgctgcta catctggaat atttcgtaca cttgctacaa gggcgcaggt
    66061 cattccgata aaggtcatgg tcgtgatttg ctttgatgaa ccagtatctt tcatctttat
    66121 tccttctttc aatagcgaca tatgtttttg ttttctttta tttttgttca agaacagaaa
    66181 tgacaaggtt tcatcgattc agtacaaaac ccagtatcac cgaaacatcc attgaacgat
    66241 cttagtgaac tgggtttgat acatgaaacg aacgatccaa cttatagaac gttttataaa
    66301 aaagaatatc ttggcgagga atatatgaaa aacaggggga aatacaattg ttttgatgaa
    66361 gcaaataatt gtctgttctc catatgtctc ttgttattct ttcacacaaa aatcagttct
    66421 attggatgat ttcgtttagt ttcttttcca ttgctttggc gatttttttt gtataaaaat
    66481 tgaaaattcg atcatcattc aaataaggag tcatgatagc cgcacgtagc agagtgattt
    66541 tctgttcttt tttccattct tctttcgtta gacccattct ttcaacaaaa cgaatcggag
    66601 aatcaccgta atcttcttgt gtaaatgtag tatgagaagt aataaaccgt tcaccataaa
    66661 catcgccatc catataagaa gaccgatcga acattttttc atttaattca ttgatagcgt
    66721 tcaaatctgt acagccttgt tctttgaata cccaattgac catattaaaa tcaggatgat
    66781 tcaatggata gacttcgatt gtttttcctt taacagtaaa agtcagatga tcgagaaatt
    66841 ctctgaatct ttgagctgcc tcgattgaag ccccaatcaa ttgaccatat ccggtcacat
    66901 tcaatggcaa tacacgatgt gctgtccaga ctgcggcggc ggttgctcca gcttttgatc
    66961 cttccaaaat ataagctcct aacatatctg gagcctttac tgatttttca aacacatatg
    67021 gcgcgaaata agaaatgata tttctcatat ttttatgttt gattacgatt cctccagcag
    67081 cataagggac atagcccatc ttatgaggat ctatcgtgac agaatccgct tctgaaatag
    67141 ctctgaaccc ttcatatacc tctttgtcaa tcgttacgca gtggtggaaa acatgatgtt
    67201 cttcaaagaa ttcagctaag gaagcatacg gcacaaattc tcctgcttcg tttagaaaca
    67261 gtgaacgcgc atagccgcca tatgcggcat caacatgtaa atagaaatag atcccttcat
    67321 cttttaacct ttctcttaat tggacgattt tatctacact gtctacttga ccttcttctg
    67381 tcgttcctac gacagcaacc acacctagaa ttgggatttt ttgtccagct aattcacgaa
    67441 ttgttttttc caaagcatta atatccattc gataatcctc ttgaactggg atagcaacca
    67501 tctgatctaa gccgactccg caaatatcta acgccttcat ccaagaatag tgttttgttt
    67561 gagggaccag ccatttccct aatctttgga tattttttcc gctacgggaa gaagctgctt
    67621 tgacctcatc catttcttcg tccgtaaagc tttcggtcat ttccaaaatt tcttcaacag
    67681 ataaattcaa aagttcccac tcagacattt ttttcacttt ttcaggcagc acttccttga
    67741 cagctaaagg aatcgatttg atgcaacggg cgtaccataa tccttcaaga ttagcaatag
    67801 aaccatctgc tgcaatgtgt ccccatccgt ctgtcatatt gaataatgaa gcaaaatcct
    67861 gtcctacttc tgcttccatt tgcgaagttg ccatagaaga ttccaatgca acattatttg
    67921 ggttccacag catcgcatag ttgtaagcta ataaagcagg catcaatgtt tcagcattca
    67981 tttgtcccca gtaacggcct gctgaatgcc atgggatcga tcctgcgcgt aatcgctgac
    68041 tgacttcatc caagacctct atcatatggt cgcgggtggc tagataatct ggagtgagct
    68101 tatctccttc gctaattgca ggcatatctg aaggaatata attttctctc catccaagat
    68161 gttcatctac catcttgttc aacaacattt tataaactgg tccattttca gctttgtcgc
    68221 ctataaagac ggccttgata tccatatctt tcatttttct tccctccata ttttgttggt
    68281 caatgtcttg cagatacatg tgtttatctg taacacctct tcactatagc gccaaaaatg
    68341 agaaattttt tttataacat caaatccacg tagattttta caaacaaaaa taaataaagt
    68401 cggatttttc tgataattca acgcaaaaaa gcgaacaatt tttcaataaa tcagtcttct
    68461 aaaatagata acctaaaaaa agcgaacaaa caaataaaat gactgtcctt cattcactaa
    68521 acatattttt atttttttat attgttaaaa attcacaatt tattttttaa tagaattatc
    68581 tgttttccaa attttataac ataaaaaacc ttttttaacg gcatttatat gctttttcag
    68641 aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat tgtgcaaaaa
    68701 aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata ttttaagaca
    68761 gctgttttta tgtgggcaat tctat
//
LOCUS       DAYYDS010000006        60890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000006 DAYYDS010000000
VERSION     DAYYDS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.006"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..525
                     /locus_tag="ACHA02_000413"
     CDS             7..525
                     /locus_tag="ACHA02_000413"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8668795.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            653..1336
                     /locus_tag="ACHA02_000414"
     CDS             653..1336
                     /locus_tag="ACHA02_000414"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8668796.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1645..1718
                     /locus_tag="ACHA02_000415"
     tRNA            1645..1718
                     /locus_tag="ACHA02_000415"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1679..1681,aa:Arg,seq:tct)
     gene            1877..2236
                     /locus_tag="ACHA02_000416"
     CDS             1877..2236
                     /locus_tag="ACHA02_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8668797.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2359..3990
                     /locus_tag="ACHA02_000417"
     CDS             2359..3990
                     /locus_tag="ACHA02_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8668798.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4052..4693)
                     /locus_tag="ACHA02_000418"
     CDS             complement(4052..4693)
                     /locus_tag="ACHA02_000418"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8668799.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4879..4955
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4975..5283
                     /gene="rplU"
                     /locus_tag="ACHA02_000419"
     CDS             4975..5283
                     /gene="rplU"
                     /locus_tag="ACHA02_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8668800.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5310..5654
                     /locus_tag="ACHA02_000420"
     CDS             5310..5654
                     /locus_tag="ACHA02_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8668801.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5667..5960
                     /gene="rpmA"
                     /locus_tag="ACHA02_000421"
     CDS             5667..5960
                     /gene="rpmA"
                     /locus_tag="ACHA02_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8668802.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6394..9201
                     /locus_tag="ACHA02_000422"
     CDS             6394..9201
                     /locus_tag="ACHA02_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8668803.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9392..9883
                     /locus_tag="ACHA02_000423"
     CDS             9392..9883
                     /locus_tag="ACHA02_000423"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8668804.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9986..10975
                     /locus_tag="ACHA02_000424"
     CDS             9986..10975
                     /locus_tag="ACHA02_000424"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8668805.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11008..11811
                     /locus_tag="ACHA02_000425"
     CDS             11008..11811
                     /locus_tag="ACHA02_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8668806.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11832..12746
                     /locus_tag="ACHA02_000426"
     CDS             11832..12746
                     /locus_tag="ACHA02_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8668807.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12829..13203
                     /locus_tag="ACHA02_000427"
     CDS             12829..13203
                     /locus_tag="ACHA02_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8668808.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13342..13587
                     /locus_tag="ACHA02_000428"
     CDS             13342..13587
                     /locus_tag="ACHA02_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8668809.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13759..14823
                     /locus_tag="ACHA02_000429"
     CDS             13759..14823
                     /locus_tag="ACHA02_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8668810.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15069..15494
                     /locus_tag="ACHA02_000430"
     CDS             15069..15494
                     /locus_tag="ACHA02_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8668811.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15487..15936
                     /gene="nusB"
                     /locus_tag="ACHA02_000431"
     CDS             15487..15936
                     /gene="nusB"
                     /locus_tag="ACHA02_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8668812.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16033..16887
                     /locus_tag="ACHA02_000432"
     CDS             16033..16887
                     /locus_tag="ACHA02_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8668813.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16888..18225
                     /gene="xseA"
                     /locus_tag="ACHA02_000433"
     CDS             16888..18225
                     /gene="xseA"
                     /locus_tag="ACHA02_000433"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8668814.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18230..18454
                     /locus_tag="ACHA02_000434"
     CDS             18230..18454
                     /locus_tag="ACHA02_000434"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8668815.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18456..19346
                     /locus_tag="ACHA02_000435"
     CDS             18456..19346
                     /locus_tag="ACHA02_000435"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8668816.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19355..20173
                     /locus_tag="ACHA02_000436"
     CDS             19355..20173
                     /locus_tag="ACHA02_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8668817.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20280..20729
                     /locus_tag="ACHA02_000437"
     CDS             20280..20729
                     /locus_tag="ACHA02_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8668818.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20747..22429
                     /gene="recN"
                     /locus_tag="ACHA02_000438"
     CDS             20747..22429
                     /gene="recN"
                     /locus_tag="ACHA02_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8668819.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22484..22600)
                     /locus_tag="ACHA02_000439"
     CDS             complement(22484..22600)
                     /locus_tag="ACHA02_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8668820.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22673..23629)
                     /locus_tag="ACHA02_000440"
     CDS             complement(22673..23629)
                     /locus_tag="ACHA02_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8668821.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23800..24168
                     /locus_tag="ACHA02_000441"
     CDS             23800..24168
                     /locus_tag="ACHA02_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8668822.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24338..24769
                     /gene="mraZ"
                     /locus_tag="ACHA02_000442"
     CDS             24338..24769
                     /gene="mraZ"
                     /locus_tag="ACHA02_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8668823.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24790..25749
                     /gene="rsmH"
                     /locus_tag="ACHA02_000443"
     CDS             24790..25749
                     /gene="rsmH"
                     /locus_tag="ACHA02_000443"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8668824.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25754..26155
                     /gene="ftsL"
                     /locus_tag="ACHA02_000444"
     CDS             25754..26155
                     /gene="ftsL"
                     /locus_tag="ACHA02_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8668825.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26156..28348
                     /locus_tag="ACHA02_000445"
     CDS             26156..28348
                     /locus_tag="ACHA02_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8668826.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28374..29336
                     /gene="mraY"
                     /locus_tag="ACHA02_000446"
     CDS             28374..29336
                     /gene="mraY"
                     /locus_tag="ACHA02_000446"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8668827.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29350..30711
                     /gene="murD"
                     /locus_tag="ACHA02_000447"
     CDS             29350..30711
                     /gene="murD"
                     /locus_tag="ACHA02_000447"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8668828.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30737..31825
                     /gene="murG"
                     /locus_tag="ACHA02_000448"
     CDS             30737..31825
                     /gene="murG"
                     /locus_tag="ACHA02_000448"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8668829.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31840..33060
                     /locus_tag="ACHA02_000449"
     CDS             31840..33060
                     /locus_tag="ACHA02_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8668830.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33216..34541
                     /gene="ftsA"
                     /locus_tag="ACHA02_000450"
     CDS             33216..34541
                     /gene="ftsA"
                     /locus_tag="ACHA02_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8668831.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34564..35805
                     /gene="ftsZ"
                     /locus_tag="ACHA02_000451"
     CDS             34564..35805
                     /gene="ftsZ"
                     /locus_tag="ACHA02_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8668832.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35822..36499
                     /locus_tag="ACHA02_000452"
     CDS             35822..36499
                     /locus_tag="ACHA02_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8668833.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36513..37112
                     /locus_tag="ACHA02_000453"
     CDS             36513..37112
                     /locus_tag="ACHA02_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8668834.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37193..37414
                     /locus_tag="ACHA02_000454"
     CDS             37193..37414
                     /locus_tag="ACHA02_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8668835.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37463..38245
                     /locus_tag="ACHA02_000455"
     CDS             37463..38245
                     /locus_tag="ACHA02_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8668836.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38266..38973
                     /locus_tag="ACHA02_000456"
     CDS             38266..38973
                     /locus_tag="ACHA02_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8668837.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39235..42033
                     /gene="ileS"
                     /locus_tag="ACHA02_000457"
     CDS             39235..42033
                     /gene="ileS"
                     /locus_tag="ACHA02_000457"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8668838.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42082..43608)
                     /gene="zwf"
                     /locus_tag="ACHA02_000458"
     CDS             complement(42082..43608)
                     /gene="zwf"
                     /locus_tag="ACHA02_000458"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8668839.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43623..44270)
                     /locus_tag="ACHA02_000459"
     CDS             complement(43623..44270)
                     /locus_tag="ACHA02_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8668840.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44454..44783)
                     /locus_tag="ACHA02_000460"
     CDS             complement(44454..44783)
                     /locus_tag="ACHA02_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8668841.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44960..45688
                     /gene="sfsA"
                     /locus_tag="ACHA02_000461"
     CDS             44960..45688
                     /gene="sfsA"
                     /locus_tag="ACHA02_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8668842.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45704..46717
                     /locus_tag="ACHA02_000462"
     CDS             45704..46717
                     /locus_tag="ACHA02_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8668843.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46717..47994
                     /locus_tag="ACHA02_000463"
     CDS             46717..47994
                     /locus_tag="ACHA02_000463"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8668844.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48057..50759
                     /locus_tag="ACHA02_000464"
     CDS             48057..50759
                     /locus_tag="ACHA02_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8668845.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50911..51228
                     /locus_tag="ACHA02_000465"
     CDS             50911..51228
                     /locus_tag="ACHA02_000465"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8668846.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51258..51578
                     /locus_tag="ACHA02_000466"
     CDS             51258..51578
                     /locus_tag="ACHA02_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8668847.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51686..53146
                     /locus_tag="ACHA02_000467"
     CDS             51686..53146
                     /locus_tag="ACHA02_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8668848.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53214..53435
                     /locus_tag="ACHA02_000468"
     CDS             53214..53435
                     /locus_tag="ACHA02_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668849.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53466..53648
                     /locus_tag="ACHA02_000469"
     CDS             53466..53648
                     /locus_tag="ACHA02_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8668850.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53648..54061
                     /locus_tag="ACHA02_000470"
     CDS             53648..54061
                     /locus_tag="ACHA02_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8668851.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54184..55365
                     /locus_tag="ACHA02_000471"
     CDS             54184..55365
                     /locus_tag="ACHA02_000471"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8668852.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55436..55600)
                     /locus_tag="ACHA02_000472"
     CDS             complement(55436..55600)
                     /locus_tag="ACHA02_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668853.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55754..55827
                     /locus_tag="ACHA02_000473"
     tRNA            55754..55827
                     /locus_tag="ACHA02_000473"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55788..55790,aa:Arg,seq:cct)
     gene            complement(55873..55986)
                     /locus_tag="ACHA02_000474"
                     /pseudo
     CDS             complement(55873..55986)
                     /locus_tag="ACHA02_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(56369..57601)
                     /locus_tag="ACHA02_000475"
     CDS             complement(56369..57601)
                     /locus_tag="ACHA02_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8668854.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57855..58424
                     /locus_tag="ACHA02_000476"
     CDS             57855..58424
                     /locus_tag="ACHA02_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8668855.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58602..59042)
                     /locus_tag="ACHA02_000477"
     CDS             complement(58602..59042)
                     /locus_tag="ACHA02_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8668856.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59200..59964
                     /gene="map"
                     /locus_tag="ACHA02_000478"
     CDS             59200..59964
                     /gene="map"
                     /locus_tag="ACHA02_000478"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8668857.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            59996..>60890
                     /locus_tag="ACHA02_000479"
     CDS             59996..>60890
                     /locus_tag="ACHA02_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8668858.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKL"
BASE COUNT        19982 a        10369 c        13044 g        17495 t
ORIGIN      
        1 aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt gattatattt
       61 ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac tccttatatt
      121 attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata tgtacgaccg
      181 aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa ttcaggaaaa
      241 aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt ttatacaaaa
      301 gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat tatcggtagt
      361 cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt tgcatggatt
      421 caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg gaataccttt
      481 gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg ttaaaaacat
      541 aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa acactcatgc
      601 agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta gtatgcgcaa
      661 tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc gtacccagca
      721 aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag cagaagtacc
      781 agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc ttcagcctga
      841 acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg ccacgacaat
      901 cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg agaaagctaa
      961 aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag gtcaagcagc
     1021 agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata gtgcaaaatc
     1081 aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg gcgttttgat
     1141 ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga ttccccgtgg
     1201 caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg atcatcccga
     1261 tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa aggaatcaga
     1321 aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg aatcgagggc
     1381 tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg atacaggaaa
     1441 tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt gataacctta
     1501 gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg gccaaaataa
     1561 ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta attatctata
     1621 attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc atccgccttc
     1681 taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt tagaaactga
     1741 ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca aaggcaaata
     1801 aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa aactcatcag
     1861 aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag ttgcaattga
     1921 atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga caagtagggt
     1981 ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt ttaagaacca
     2041 aggagtgtac aatggactat taggagtggg actactttat ggagcttatc tttcttccgc
     2101 atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat tatatggcag
     2161 tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt tggcattgct
     2221 ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa ctttgtgaaa
     2281 aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag aaaatttcat
     2341 gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa agaattttca
     2401 ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt agctgctgta
     2461 gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc cgcaggtttg
     2521 attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt ttatcaaata
     2581 tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac aaagggaatg
     2641 caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc aggacttttc
     2701 cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt tacttcaggt
     2761 attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca ctcggaaggt
     2821 acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat aaacacagcc
     2881 tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa aaaatgggcg
     2941 aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat gctattgttc
     3001 catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag taatcgtttg
     3061 aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc tattagcatt
     3121 gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg aatggcaaat
     3181 aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct gttgcttcct
     3241 ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc catcaaatct
     3301 ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct ctccatgctt
     3361 gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt tttgattgtg
     3421 acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa taaatattgg
     3481 tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga tttaagtatt
     3541 gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag cgctgctatc
     3601 acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac ggaaaagctg
     3661 aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc agaaaaactc
     3721 aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat gcgaggcgta
     3781 ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa agcagagcaa
     3841 agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca actttctcac
     3901 ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt gacagcttta
     3961 catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac aagactgtaa
     4021 actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt gaagctagta
     4081 cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata gctgctggat
     4141 gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc ttttgttctg
     4201 ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc gtatattctt
     4261 tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt ggctggacct
     4321 tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga gtaggtgtcc
     4381 gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga gtcacgcgat
     4441 acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag ccagttgaag
     4501 aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc gtatcaatct
     4561 ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt ccttgcccag
     4621 cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt atcagttctt
     4681 ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa gaaacacaat
     4741 gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg cttgaatttt
     4801 tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg taagctagta
     4861 ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa gcacagcata
     4921 tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta tagcatgtac
     4981 gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc aatctatgtt
     5041 gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat cttagtaggc
     5101 ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga aggtactgtt
     5161 gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa aaaacattca
     5221 caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc gatcaacgct
     5281 taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag cgaaatgacg
     5341 caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct tatggaagtg
     5401 atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg attgcctcat
     5461 tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat ctttataccg
     5521 aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta ttagaaaatc
     5581 ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa attaaaacaa
     5641 ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa ttatttgccc
     5701 acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa cgtctaggtg
     5761 ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt caacgcggta
     5821 caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta tttgcaaaag
     5881 ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg tctgtttatc
     5941 cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca agacttttgt
     6001 cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag tttactttgt
     6061 ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat aactatcgtt
     6121 ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg taaacagtgt
     6181 tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt taaattgaac
     6241 atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt gacgaaacag
     6301 aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag aaaaggagcg
     6361 ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa aaccgctcat
     6421 ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga agtgttaggg
     6481 attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg aaaactgata
     6541 aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca cgtaaagcca
     6601 gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa aaatacactc
     6661 caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag catgaaaaat
     6721 tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa ttgtttgatt
     6781 acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca ttttgcaaaa
     6841 gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc tgattatgcc
     6901 aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc ttttacaggc
     6961 gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct gtttttggat
     7021 gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat ggatcatgga
     7081 acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg gatcgtaggt
     7141 gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg tatcccaatc
     7201 aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga tttagtaaaa
     7261 atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac agaagatgta
     7321 gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa gtcaaatatc
     7381 cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat ctcgatcaca
     7441 atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag caaccgcagt
     7501 gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc aaatgatacg
     7561 atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga tatcatcgga
     7621 gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa tcatttcatt
     7681 tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt ttcttttaat
     7741 gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac caatcagatt
     7801 tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat ttatgctatg
     7861 agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg gcatacgaat
     7921 gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc gaaagaagtg
     7981 cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga ctattacttg
     8041 accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg tgtagtcgtt
     8101 gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca gttgttagat
     8161 gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac agcattggac
     8221 cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact cttagtagat
     8281 atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat tccagtacgt
     8341 actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt ctcagtatta
     8401 gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta cgcagaaata
     8461 tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc tgttttggct
     8521 atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga acgagcggta
     8581 aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga agtgaaagaa
     8641 gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat tactgatttg
     8701 aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat cgaagtcatt
     8761 ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc cctagatgaa
     8821 gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt tattaatggt
     8881 agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac gacgggaatc
     8941 ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg aatgatcgaa
     9001 cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa tacaacgaat
     9061 gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca gataaaagta
     9121 aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca gttagataaa
     9181 aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg tatcgttttt
     9241 gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt tggcacgctt
     9301 tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat aggggtttct
     9361 gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac gtatagatga
     9421 tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg ttgaacgtat
     9481 cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac taaaagaagc
     9541 tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc aaatctatca
     9601 tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc aagatgttga
     9661 agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta tgagttttac
     9721 tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata ttgattcctt
     9781 tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg ctgacaggaa
     9841 aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat ggttgttgga
     9901 aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga atttgttaca
     9961 acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc atggacaatt
    10021 tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa acgtcaaagc
    10081 cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg aagaagcggt
    10141 tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg gcggaacacc
    10201 gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa tcgtcagcgg
    10261 tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg aatctgctca
    10321 tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa aaccggaaga
    10381 attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta aaggagcact
    10441 tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac gtgttgactc
    10501 acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac caaaccgtat
    10561 tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa tcgaacaagc
    10621 tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg aagtagcaaa
    10681 agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg aagacgtatt
    10741 gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa tggcgcattc
    10801 ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg ttgaagcgtt
    10861 tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta atgattcacg
    10921 cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag cgtaataaat
    10981 aatattaaaa tttataggag gcttatcatg tctattattt caataatttt agtcgtatta
    11041 gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca tcaaccacta
    11101 gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat cgtacttggc
    11161 ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt agcaccagat
    11221 gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca aggtgttaaa
    11281 ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt gttcttaaca
    11341 atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc tgctgaagaa
    11401 ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca aggggttcgt
    11461 atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca atcattcctt
    11521 gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat ggtcgtagct
    11581 gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc attcttcgtt
    11641 attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg tgctttaggt
    11701 gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc aaataatgga
    11761 ggcggaagca actctggtga ccctttaggc gacattttga atgactatta atcttgaagg
    11821 aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt ttagctgttg
    11881 cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa aacggtggct
    11941 gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa gaacgttctg
    12001 cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct tctccaattt
    12061 taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt gatgatgtag
    12121 cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt gacccagtgt
    12181 tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct atgggaggaa
    12241 atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg gcattcatgt
    12301 ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac ctttctggcg
    12361 gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta ttagctgccc
    12421 ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta aaattagata
    12481 aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc aagagtgcat
    12541 ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca ttacaagaca
    12601 acttagacca attgattcct ggtttagctg cattaggatt aacattgtta tgtatgtggt
    12661 tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta gtcggtgttg
    12721 ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt tgtcccgagc
    12781 tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat ggttcaatca
    12841 atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct gacagattac
    12901 gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga tagacgcaaa
    12961 tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat gctaaaggga
    13021 aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac attttcttcc
    13081 aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga tagaatggta
    13141 caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt taaacgcaag
    13201 taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt tgcagaagaa
    13261 aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct aaaattgatt
    13321 agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg agccgtgatc
    13381 ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt aacgaatatc
    13441 attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg gggaccaaaa
    13501 gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt agcagttgtc
    13561 tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa aaggagttgc
    13621 agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag ttttccggaa
    13681 atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa agcgtgcaaa
    13741 gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa aatgcaagaa
    13801 gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt aacgaacttt
    13861 acaggtacga caggattagc tgttattact ttggaaaaag cattttttat tactgatttt
    13921 cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa aaatgtcgga
    13981 cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc attagctttt
    14041 gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat cgatgcggaa
    14101 ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga agaagaaatc
    14161 gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat cctgaaaatg
    14221 atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta tatgcgttct
    14281 cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg ttcggctatg
    14341 ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac attggacttt
    14401 ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat tggtgatcct
    14461 ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc agttattgat
    14521 gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga ttatatttca
    14581 aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg tttagagatc
    14641 cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg aaatgtgatc
    14701 actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga ggatgacttg
    14761 ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt gatcatctta
    14821 tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt tgttatagaa
    14881 aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag ataataaagt
    14941 tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt tcttaccacg
    15001 tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc aaagggagga
    15061 ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat ttaggagaga
    15121 tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct aaagtagaag
    15181 gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta ggacgtgcag
    15241 ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta gatctttact
    15301 gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag gaacgggtca
    15361 gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc catgtagtag
    15421 ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa gacgaagagg
    15481 aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt gcaggcttta
    15541 tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa cgcaatcgaa
    15601 ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt ttatcttgat
    15661 acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat tgaaaaacat
    15721 ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat cttgcgtatc
    15781 gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt aaatgaagca
    15841 atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa cggtgtttta
    15901 tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt ctatgaatca
    15961 tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca aaaaatatct
    16021 gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa aatgcaggca
    16081 gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg attagtcgtt
    16141 ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga acgtgtagca
    16201 aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc tgaagacgaa
    16261 ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat attggtccaa
    16321 ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg ccctgaaaaa
    16381 gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa accggacaag
    16441 attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat cgatccggcg
    16501 ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat ggctcagcta
    16561 ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa tctggctgaa
    16621 ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt tgtcacaaaa
    16681 gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga tgctaacggt
    16741 aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg gatcactcct
    16801 gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac cgtaaaatct
    16861 gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt cactacatta
    16921 acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt atatttaaca
    16981 ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag cttaaaagat
    17041 gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt acgattccag
    17101 ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga agcgggaggt
    17161 tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt atatcaagaa
    17221 ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc taaaaaaatg
    17281 ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc ggttatccgc
    17341 gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt gttcccaaca
    17401 gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag agtagaagag
    17461 atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga ggatttatgg
    17521 ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc ggtgatttca
    17581 tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt tcgggctgcc
    17641 actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt gatgcgcatt
    17701 caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg caaacaagaa
    17761 cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt gtatgaaggt
    17821 cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca gcaaatttat
    17881 catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt ggctccgctt
    17941 tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt agaagagaaa
    18001 atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca atctcttgat
    18061 ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt aaatgatcaa
    18121 gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca ttatcaagat
    18181 ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata tggaaaatcc
    18241 aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag aacaaggaga
    18301 cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa gcaaacattg
    18361 ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag aatcaaatga
    18421 agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac ttttcagcta
    18481 ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat acaacggata
    18541 gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga atacgtccct
    18601 tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc gtctatcaag
    18661 tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat ttgcctgcga
    18721 tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc ggcgaagcat
    18781 tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc agtatggcac
    18841 acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt gcgggatcac
    18901 aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag ctttctttag
    18961 aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat gccttgctgg
    19021 ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa cgattagccg
    19081 aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt ggtacaacga
    19141 aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact tacccgcgtt
    19201 tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc gccaacaaga
    19261 tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg cgccagatga
    19321 tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg tagatgtatt
    19381 agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag tgatggctgg
    19441 tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa ttccagtgag
    19501 tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg gattaaaatt
    19561 agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt tagatattgg
    19621 tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa tgagttatgc
    19681 tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta gagtcgtcgt
    19741 aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag gaattccaga
    19801 agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc cattacatgc
    19861 tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg aagcaggcag
    19921 agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa aagttttaga
    19981 agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa gttattctcc
    20041 gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag tacccgaaag
    20101 cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac acgaacagtt
    20161 tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat tcttctttat
    20221 tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc gaagacgtaa
    20281 tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa gatatccgaa
    20341 agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact caagctacga
    20401 tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat ggcggctacc
    20461 gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa aaactattaa
    20521 aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa acattgccgg
    20581 gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa ttattttcga
    20641 ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag gcagttttat
    20701 tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc tacaagaaat
    20761 cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg aaggaatgac
    20821 cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt taggtctgtt
    20881 agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat gtgtgcttga
    20941 agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg aattagggat
    21001 cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg gaaaaaatgt
    21061 ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg gcagttattt
    21121 agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat cacatcttgc
    21181 tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt atcagcaaga
    21241 atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa atgaaaaatc
    21301 ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg ctgctgattt
    21361 gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca attatcaaaa
    21421 aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac aaaacagtgt
    21481 agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag atgtggagta
    21541 tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg caatcggcga
    21601 tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag aagttacgca
    21661 acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg agtcaatatt
    21721 ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag aaggtcaatt
    21781 ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac aagccgcaga
    21841 tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc tgcatgaatt
    21901 gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag aagaaaatag
    21961 acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca atccaggaga
    22021 gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga tgttgcttgc
    22081 tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg atgaagtgga
    22141 tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc aaatatcgaa
    22201 atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg ccgactatca
    22261 atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt cagagttgaa
    22321 aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga ttacaaaact
    22381 cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag ccacctcgca
    22441 gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg acactcatta
    22501 cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg acaaattttg
    22561 ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac ctcgtcatta
    22621 attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt ttttataaaa
    22681 acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt aatagaacta
    22741 gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt ccatagatac
    22801 tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt ttcataatat
    22861 tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct actagtttta
    22921 attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca cgtaaatgat
    22981 ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc aatgcatcta
    23041 aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc atgatttgat
    23101 aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg cattgagatt
    23161 gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat tgaaagaaga
    23221 tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact ttctttaaaa
    23281 acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa ataatagaga
    23341 gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa ggatattgta
    23401 acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta cgggaatttt
    23461 ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga tgtgtcagct
    23521 gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg tcagtttcct
    23581 ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg attttcctcc
    23641 tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa tgtgtttgta
    23701 agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta aagtatatac
    23761 agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt cacaccactt
    23821 atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt tcttgtaaaa
    23881 ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat tccttttttg
    23941 tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt accgtttttc
    24001 tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata ttattatgaa
    24061 gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct gttgacaatc
    24121 gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa aaaatagaaa
    24181 agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa atgtttatga
    24241 ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg gattgtggta
    24301 gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg aattccaaca
    24361 taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag aacttggcga
    24421 aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga tgagcgaatg
    24481 ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg cacgtacttt
    24541 tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag gacgaatcaa
    24601 tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag tcatcggtgt
    24661 atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg cagcagcaga
    24721 agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag aataggaaga
    24781 ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga aacggtagat
    24841 ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg tgccggacat
    24901 agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt tgaccaagat
    24961 caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca aggtatggtc
    25021 acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca tgtagataag
    25081 gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga agcagaacgt
    25141 ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc agctccttta
    25201 tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat tttcttccgc
    25261 tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt acgaaaaaca
    25321 aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat cccagctccc
    25381 gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg gattgcagta
    25441 aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt attgaaagtc
    25501 aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt caaaagtatg
    25561 tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat acctgacgaa
    25621 ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga ggaagagcta
    25681 acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat aagaatgaaa
    25741 gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg atttacctga
    25801 attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag aaatcattct
    25861 tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa ttgctgtggc
    25921 aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc gcacaaatat
    25981 cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata aaaagcaaga
    26041 aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga ttaaacaaat
    26101 cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga agtaaatgag
    26161 tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga tgaacaatcg
    26221 caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt tatttgccgt
    26281 ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag ctgaaaaaac
    26341 aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga tttatgatcg
    26401 taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga tactctcaaa
    26461 aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg ataaaatagc
    26521 agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata ttttagagga
    26581 cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa atatctccca
    26641 agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg gtctttattt
    26701 tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt ttattggata
    26761 cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg agtctgctta
    26821 caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata attttcaaaa
    26881 tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag atatctacac
    26941 gactttagac agcagactac aaagttattt agaaacactg atggatcaag taaatgaaga
    27001 atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag aaatcttggc
    27061 aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg gaaaagatgc
    27121 tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca tgaaagtttt
    27181 cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct tccaatctgg
    27241 aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa aaggtgtgct
    27301 gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac tggaacagcg
    27361 attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa gtactcattc
    27421 tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg accgagcaat
    27481 gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag ctttcacttc
    27541 tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg tggatcccca
    27601 aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt caaaagaaac
    27661 aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact acggtagtgc
    27721 ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa cagcccaaat
    27781 cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt cgattgtgga
    27841 gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac accctaagac
    27901 atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg caatggattt
    27961 taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat ccgtagccga
    28021 ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg gactgcaacc
    28081 tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg gtgatcagtt
    28141 gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc ctgacgtaac
    28201 aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg aagttagttt
    28261 tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa tcactgaaga
    28321 caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag agcatggaat
    28381 ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc gcaatgccgc
    28441 tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag gagggaccaa
    28501 agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt ctgatcggct
    28561 ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct actcttttca
    28621 tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat tttataaaaa
    28681 tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga cagattctag
    28741 ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta ttgaatttct
    28801 tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc tggcttgttg
    28861 gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct ggcttaggaa
    28921 gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat gatgtattaa
    28981 tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac agaaaacctg
    29041 ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta gcggcaattt
    29101 ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat gttatggaaa
    29161 cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga atttttaaaa
    29221 tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag attgatacag
    29281 ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt tggtaggaga
    29341 tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt cttaggactc
    29401 gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact tgtcaccgtt
    29461 aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc tttagggata
    29521 aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc attgatggtc
    29581 aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga aatgggtatt
    29641 ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat tattgggatt
    29701 acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact gaatgcagga
    29761 gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag tggtgtagcg
    29821 caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca actcatggga
    29881 atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc acatattgat
    29941 tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa aaacatgaca
    30001 gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt agcgcaaaga
    30061 acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg tgtgtatgct
    30121 gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga acttggcgta
    30181 ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa attgtatggt
    30241 atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc acatcgtacg
    30301 caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc aaccaatatt
    30361 ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt attggctggc
    30421 ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg aatcaaagca
    30481 atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa agcaggcatc
    30541 gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt agaaaacagt
    30601 acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca atacccaaat
    30661 tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata gaaaaagtag
    30721 aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg gacatattta
    30781 tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg aattcatgta
    30841 tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa tgcctttcca
    30901 taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca agacggtccg
    30961 attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc aaccagatat
    31021 cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag ccaaaatggg
    31081 tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata aatttttaag
    31141 ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt ttcctgaaaa
    31201 caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta aaaaatcaga
    31261 tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt ttggcggtag
    31321 tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat atattccttc
    31381 ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc taaataaaat
    31441 aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg cagaggtcat
    31501 ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag aattcacagc
    31561 tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc atcagactaa
    31621 aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata atgaactaac
    31681 aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat tgcaaaagca
    31741 aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc tttatgactt
    31801 agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa aaaagaacca
    31861 aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca aaaggctaac
    31921 cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga agacataaca
    31981 ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga acagcaagaa
    32041 gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa gaaaaacggt
    32101 ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca acggcacaaa
    32161 gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct gatttttttg
    32221 atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg caataagaca
    32281 gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa tgtttgggga
    32341 cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc aagaatcgaa
    32401 acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac agaatataaa
    32461 gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa tggaacagta
    32521 ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga gttcaaagac
    32581 tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga attacaaaaa
    32641 gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat ccaattgaat
    32701 atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca aatgaaatat
    32761 tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga agttggtatc
    32821 ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc ggaagaaaca
    32881 actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc agaaacgcaa
    32941 aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa tgattcaagt
    33001 gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa tgaaaattga
    33061 taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta tggtagaatt
    33121 ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag ttgttcgctt
    33181 gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga atgtatgtag
    33241 gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc gacagccaaa
    33301 tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc attatcgttg
    33361 atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa gaaaaagcag
    33421 gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa gtagaaaatt
    33481 gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa gatgtcagaa
    33541 atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc gtgtcaatat
    33601 tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt ggaatgatcg
    33661 gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc atccataaca
    33721 tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt actcctttag
    33781 cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc gtcattgata
    33841 tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag tttaccaatc
    33901 ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta aatacgtcat
    33961 ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa cggacatcac
    34021 cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa gtcgatgaac
    34081 gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa gcaaaagatg
    34141 cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca ggcggagctg
    34201 ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat gtaaaattgt
    34261 atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata agtatcgtgg
    34321 attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt acgggtgaaa
    34381 cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat gagagctatg
    34441 aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga gaaaatgtaa
    34501 aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg ggaggaataa
    34561 gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa agtcatcgga
    34621 gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt taaaggtgtt
    34681 gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc tgaaacagtt
    34741 atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc agaagttgga
    34801 caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg tgcagatatg
    34861 attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc aatcgttgca
    34921 ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc attcactttt
    34981 gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa agaaaatgtg
    35041 gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa aaagacaccg
    35101 atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca aggtatctct
    35161 gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa aacagtgatg
    35221 gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga tcgcgtagta
    35281 gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga tggagctgaa
    35341 caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc acaagatgct
    35401 tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg tacatctatc
    35461 aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat cgatgaatca
    35521 aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc acctgctcaa
    35581 aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga aaacagtttt
    35641 ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga ttccaatttt
    35701 gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa atctcatggc
    35761 gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag aggaagagca
    35821 catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt gtgctcttgt
    35881 tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg aatccgctca
    35941 agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag tagataaatt
    36001 attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt taattggtaa
    36061 tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc atgcgttgga
    36121 cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat cctgttttat
    36181 tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg agcttttacc
    36241 gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga tgactatggc
    36301 gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga aagaattgcg
    36361 tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa gtatggggat
    36421 gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg taggaacagc
    36481 gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga ggagcaattg
    36541 ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat cctgaaacaa
    36601 caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca actcattctg
    36661 tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct gtaacgcatg
    36721 aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca cctacaaaaa
    36781 ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca aatcttgctg
    36841 gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt gctaaacgaa
    36901 tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa caagcgagaa
    36961 gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc aaacgtgttg
    37021 gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca accgctcaat
    37081 cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt atactattgc
    37141 actattactt catttacttc aacgggcagc ctatttctat agcattatct tgatggtata
    37201 tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat tattagctaa
    37261 gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg gacccttgga
    37321 tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt tagcacagat
    37381 cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc tttattctca
    37441 gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc gggcagatga
    37501 acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac aatatgcacc
    37561 ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac tgattcgaca
    37621 aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa gaaaaagatg
    37681 tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag cattgtatga
    37741 aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg gaactttagt
    37801 gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg atcgctggca
    37861 agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa agattggaaa
    37921 ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga aagatagctg
    37981 gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct ctgagattta
    38041 taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa aagtgaactg
    38101 gacagaaaat acaagacctg attttgtact tgaactatta gatattattt cagtcagagg
    38161 ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa agtacagggt
    38221 actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc tttaactcca
    38281 ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca agacgaagta
    38341 gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag aaatcgagaa
    38401 ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga attaaaagat
    38461 gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa aacaagtgca
    38521 agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga gttagttgcg
    38581 aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc aagaaaaatc
    38641 ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga tcttaagaaa
    38701 aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt agaacaagtg
    38761 aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac tgacagccat
    38821 gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac tgatgtaaac
    38881 tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc tattgatatc
    38941 tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg ttattcttaa
    39001 agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat cctctacttg
    39061 agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat tggtcacaga
    39121 gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt tcgtcccttg
    39181 cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg ttgaatgaaa
    39241 atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa tttgccaaac
    39301 cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa aagacaagag
    39361 ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc caatggtaat
    39421 atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg ttcaaaatca
    39481 atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt gccgattgaa
    39541 caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga atacttggaa
    39601 aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt caaacgccta
    39661 ggagttgcag gagattggga acatccttat gttacattag atccttctta tgaagctgca
    39721 caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg attgaaacca
    39781 atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga atataaagat
    39841 gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga tattcttgat
    39901 gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc aaaccaaggt
    39961 attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg taagtttgtc
    40021 gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa tgtaacagtt
    40081 ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc attctatgaa
    40141 cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac tggattagtc
    40201 catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata taaactatct
    40261 gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg atttgaaggt
    40321 atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa aggagctttg
    40381 ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa aaagcctgtg
    40441 atctatcggg caactccaca atggtttgca tctatcgata aattccgtca gaatattttg
    40501 gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt gtataatatg
    40561 atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt accacttccg
    40621 attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga acatgtggca
    40681 aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa agaacttctt
    40741 cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga aaaagatatc
    40801 atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca gcgaccagaa
    40861 ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg ctggttcaac
    40921 tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc agttatttct
    40981 caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg aaacatcatt
    41041 gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg ggtatctagt
    41101 gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt ggcagaagta
    41161 tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga tttcgatcct
    41221 aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat gaccgttcga
    41281 ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa cttcatgcat
    41341 atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta cctggatttt
    41401 gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg catgcaaact
    41461 gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat tccgcataca
    41521 gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt aagcgagttt
    41581 cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac tgctttcatg
    41641 gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa attgattggt
    41701 aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga aatgttaaaa
    41761 gctttggatg cagatattgc acaattattg atcgtttcac cagactactt tgaagttatg
    41821 gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc tattacagtg
    41881 gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga tgtaggagta
    41941 gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga cttctatcct
    42001 gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt gacatgttct
    42061 ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat accagttatc
    42121 tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg ctttaggtcc
    42181 cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag cttttcgaat
    42241 gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt tcgttccatc
    42301 accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt caaccatttc
    42361 tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc ctacttcttt
    42421 cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta gaacattagg
    42481 tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat taacaggaac
    42541 ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc ctgtacgtac
    42601 atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg cgacgaatgt
    42661 ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga attgtacacc
    42721 atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt cttcttcgtt
    42781 ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt tttcggaaaa
    42841 tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat tttgcaccat
    42901 gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac cgaggttttc
    42961 gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct caaaaatatt
    43021 attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat aatgatcaat
    43081 cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt caaaagcaga
    43141 ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat tttttgtcat
    43201 aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg ccatagccaa
    43261 ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag acaattcttt
    43321 taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa aatggcttgc
    43381 aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt tgacaacttc
    43441 acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa aatgctcgcc
    43501 taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct gggctaggtc
    43561 acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt ggatacactc
    43621 ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg gattttcttt
    43681 ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat ctcatattct
    43741 tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc atcaagcact
    43801 cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg gcgttcttca
    43861 tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc aggatgattc
    43921 aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa tacttcagca
    43981 tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac ttcccataag
    44041 cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa ttgtacacct
    44101 tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat ttcactgaca
    44161 gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt cgttgtatct
    44221 ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat attcgatcat
    44281 cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta attgactatt
    44341 tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg aaagatgata
    44401 gagaagactc attctttcgt ttataaagga tatatatata cattgggagg gatctatttt
    44461 tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg ttcgggataa
    44521 gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc atctatttct
    44581 accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga tgtaatctgt
    44641 aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt tgagagtcct
    44701 tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag aacggtaatg
    44761 agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta tgagaaaata
    44821 atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc taaaatttct
    44881 ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg atacatagta
    44941 ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt ttattgaccg
    45001 cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta ttgcgcatgt
    45061 caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg ctattgtatt
    45121 tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa agaagggtga
    45181 ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta ttttagacgg
    45241 cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg aagtaactta
    45301 tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag catttgttga
    45361 agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg cgcccacact
    45421 cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt atgccgctta
    45481 tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg aagaaatgca
    45541 accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa tcctcgccta
    45601 taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat ttgatgtaga
    45661 tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca acttaggtat
    45721 tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca caaaaaaata
    45781 tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg gttctcatta
    45841 tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta ctcatatgga
    45901 caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc aagcaatttt
    45961 ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag aaatggatga
    46021 aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa gaaatatcca
    46081 tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga taatcggtgc
    46141 aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg gagaagaacc
    46201 gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg tttaccttgt
    46261 atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc caagaaagat
    46321 ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt ttgatgtatc
    46381 gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt ttgactcagg
    46441 aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg atcgcgcgca
    46501 taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag atgaagcaga
    46561 ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt tgtatgaaga
    46621 gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa aaaatggcgg
    46681 catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt acaagtattt
    46741 cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc tcttattcaa
    46801 cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc tcctacaggt
    46861 tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc aggtgaagca
    46921 agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc agatgttgca
    46981 cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg agccaacgtc
    47041 aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac accaggaaga
    47101 atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac aatggtattt
    47161 gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca aattcttcat
    47221 caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg ttcccttgaa
    47281 agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc agaggacgat
    47341 tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa agatgactac
    47401 ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa tcaactgact
    47461 gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg aagtctggcc
    47521 tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa gaaaatggtc
    47581 agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg tcttccttat
    47641 gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc gggaagaact
    47701 ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa tctaagagat
    47761 ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt atacggaggt
    47821 aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa taaaaagtat
    47881 gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa aataagaaaa
    47941 aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa ataagcgcct
    48001 ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg ggttatgtga
    48061 aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga ttgacagcaa
    48121 gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat ttaagtgaac
    48181 tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac tctttgccaa
    48241 cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg acttttgaaa
    48301 cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa gctgcgattc
    48361 tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca gggaagtcga
    48421 tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct gcagatgcgc
    48481 catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc tttggacaca
    48541 tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc ggtctaatag
    48601 ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg ccggaagggc
    48661 aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga gaaagtgcac
    48721 aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca gaaagtttct
    48781 tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt gcttcacgtt
    48841 cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg aatcgtttga
    48901 atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat gatgcggaag
    48961 gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct tttttacatt
    49021 accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc cttcaagtac
    49081 aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc atggacagca
    49141 aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca caagatccag
    49201 aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt tccgaaaccg
    49261 gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct tatttccaga
    49321 catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca gatgaaatct
    49381 ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt atttataatg
    49441 aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt gtgagagaag
    49501 gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag agggaattcc
    49561 aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag attccttttg
    49621 atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt gagcctgtta
    49681 aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct tcaagcatgt
    49741 tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat atgccactgg
    49801 ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac tatcaagagc
    49861 atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc ttcagtgatt
    49921 tgatttatga agagactggc attcggacga aagctatcac gatgacaagt accatgattg
    49981 tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt tatcaaaata
    50041 tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag tctgaaactt
    50101 tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta gctgccaaat
    50161 tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc cagatgcagt
    50221 ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa tatgagatca
    50281 aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt tcagcctatg
    50341 acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat gagatcccga
    50401 cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct attgatgaac
    50461 tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg attatcgtgg
    50521 aacctggagt agatactgga atcgttatcc atttagcctt catgttagac ggattgatca
    50581 aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt tatcgtttgg
    50641 aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag gtaaaaattc
    50701 cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata aaaaaatgaa
    50761 cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa aaagagctaa
    50821 aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg cgttatataa
    50881 aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat gttagtttgt
    50941 tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc agaagaaaaa
    51001 gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa tttagaatct
    51061 aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc tcaatttgaa
    51121 caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga ctatggcatg
    51181 atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat caagtatgca
    51241 gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg gggttaatca
    51301 tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct aaaatcggtg
    51361 attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata caagcgcatc
    51421 atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa gtgacattat
    51481 tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat ttagcaaaag
    51541 aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt ttttgaacgg
    51601 aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc tctttatata
    51661 aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg agaaatacat
    51721 cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc gtgatgcttt
    51781 tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca atgcaattat
    51841 tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag cagacaaagg
    51901 aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga acggtacact
    51961 agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc gagcttatgg
    52021 agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag gtatttcctt
    52081 tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg cagatgcaat
    52141 caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg gattatgggg
    52201 ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga tcatgggagc
    52261 tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca aaatgcctga
    52321 ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga cgatcgcttt
    52381 atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc aactgatcat
    52441 tgatttggtg caaaaatata ttgcagagcc attcctagga ttatctcaag ggttgggagc
    52501 agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc atggaccaaa
    52561 tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca atattgatat
    52621 tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg caatcgaaga
    52681 tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt ttggcggatc
    52741 aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg cagattatct
    52801 aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac cagtgatgtt
    52861 tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg ctccagtagt
    52921 agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg tatcgcagca
    52981 agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag cggattggcg
    53041 ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg caccatttgt
    53101 catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg aacgtgagga
    53161 gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact gaaatgatta
    53221 caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga tacaatgaag
    53281 aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt gaaacaagtg
    53341 cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca cagccttggg
    53401 gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa gataggatgg
    53461 ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc gtgttgtata
    53521 gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt ctttttctaa
    53581 caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca aaggagaaaa
    53641 ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt acgataaagt
    53701 tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa ctagaaaaca
    53761 gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc gcataaaaat
    53821 cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga agaatgaatt
    53881 cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc attacacaga
    53941 aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag gaacaatctt
    54001 gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag caacgtatta
    54061 aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag aaggaatttt
    54121 tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg aaggggcagc
    54181 aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat gtttgattta
    54241 gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt tcgtacactt
    54301 gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac aagtcaagtc
    54361 atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc cggcattggc
    54421 tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat catggaaaag
    54481 atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc attctcctat
    54541 cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta tcaactaaac
    54601 agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat ggattttgaa
    54661 gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca acgagctggt
    54721 ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa ttaccagttt
    54781 gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca gcttgcaacg
    54841 tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg cagccttgtt
    54901 cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg cggctacggc
    54961 ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat cacaccttat
    55021 atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt taaaaagaga
    55081 caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa tgaaggaatc
    55141 gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca aacaaaactc
    55201 ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta tcgtaaagtg
    55261 gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga aacgttaagc
    55321 tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa aacgggtgat
    55381 gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga aaatgttaaa
    55441 gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta gggttttctt
    55501 ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta gatgaatcta
    55561 agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac ctccctacat
    55621 tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt tttgcctaac
    55681 ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa gctacaattg
    55741 atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct aagcgatagg
    55801 tcgctggttc aattccagtc aggaacgtac aaataacccc tgtatccttt gcggtacagg
    55861 gggttatttt gtataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga
    55921 tacgctaagt atatttactc cttgataaag taaaatagat gcatgcgtat gtcttagtcc
    55981 atcaataaac agtcgaagag aaaataggga aaaattgatt actaaagaaa aacttactag
    56041 gtcgacggtg tagttgattt tcatacttca atagattcga attaaacgat ttcgattggc
    56101 ttataccagt cctatccaac gaaagatata tcgttcttaa ctctagcggt ataacttgaa
    56161 gctttacgta aagccaatgg gaatgaactg gagatgactt tttattattt ttagtaatag
    56221 ctgatgggtt aatacttaca actttccttt tcaaagtacc atataaaaaa acggtggggt
    56281 tgtttagcga aataaaggat ctaagccaat agcttagatc ctttattcta tgctttatat
    56341 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
    56401 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
    56461 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
    56521 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
    56581 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
    56641 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
    56701 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
    56761 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
    56821 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
    56881 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
    56941 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
    57001 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
    57061 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
    57121 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
    57181 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
    57241 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
    57301 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
    57361 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
    57421 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
    57481 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
    57541 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
    57601 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
    57661 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
    57721 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
    57781 tatcaaaaaa acatgttaca atgaaattaa tcaatggtgg aagcgttata aaaaaattaa
    57841 ggagattaaa aaatatggca agaaaaaaga cgattactag agaacagatt ttggcagctg
    57901 cttatgaagt agctgccacc gatggattta cacgatttac tgcgagaaac atcgcgaaca
    57961 aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa aaatatggat gacttgagac
    58021 aagctctttt tgagcagatc tatgatcatt tacaatatga agtgttcact gttgagcata
    58081 cagggaatac gattatcgat cttgctttta attatatcca ttttgcaaga agagagaaga
    58141 agttgtatcg ttcactgtac ttagaagaat atggtggagg aaaagagatg cagaatttct
    58201 cttatactta ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat ctttcagaag
    58261 agcagatcga atctttgtat aatgggacat ggatcatagc tactgggatc gcagctttga
    58321 tgtcttcaag catcatccat ccaactgatg aacagattga aaagatgatc caagacagta
    58381 tagatgctat cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac tcaaaagttc
    58441 cacaaatcag taggtaacaa gagatgtgtc aaaataagga aaagttcgtt tttcttacag
    58501 cgtatgcatt tttaactccc gaagaactct tccatgaatg caatgaataa aagagccata
    58561 cagattgaat aaatctggat ggctctttta ttgcaagtat attaaatttt tgcgactagt
    58621 tctttagcga aagcttctaa attttcgata tcttcttctt cagcattcag atccacttta
    58681 acattttcgg ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa atcatccacc
    58741 gatttgcaga attcatcgta aaaagtatca ccagatccgc agacaccgta aatttttccc
    58801 aatagatcca attcttgcaa gtcttcataa aagtctacga tctcatcagg cagatcgcca
    58861 tcatcgtatg tataagtagc tactacgcaa atatctgcct cctcgaattc atctgcatct
    58921 acttgggtac attcgttgat ttcgacttcg atatccagat tttcaaatgc ttctgcaaca
    58981 atatcggcga tttcttcagt gtttcctgtc atgctagcat atacgatttt tgctaaagcc
    59041 atgatgtgcc tcctcgttta taattattta acaattatga attataccaa aacatcattg
    59101 aaaagaaaat aaaacctaaa gacacctggg gaatgtcatt tggctaaatg tatgccgcta
    59161 tgttaaaatg agagggattt gtaaaaagga gacgaaaata tgattacttt aaaatcagca
    59221 cgtgaaattg aaatgatggc agaatctgga gctttattag cagatgtaca taaaaatctg
    59281 cgtgatttta taaaaccagg aatcacaagt tgggatattg aggtatttgt cagaaactat
    59341 atcgagagtc atggcggaat tgctgcgcaa attggctttg aagggtatga atatgcgact
    59401 tgtatcagta taaatgatga aatttgtcat ggattccctc gtaaaaagcc tttgaaaaac
    59461 ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag gcggcatttc tgattcttgt
    59521 tgggcctatg tagttggaga atctacacct gagatcgacc gattgatgga agtaacgaag
    59581 aaagctttgt acatcggtat tgagcaagca caagtaggga atcggattgg tgacatcggt
    59641 catgctatcc aaacttatgt agaaggcgaa aatctagcga tcgttcgtga ttttattggt
    59701 catggcgttg gtcctacgat tcatgaagaa ccagcagttc ctcattacgg agaagctgga
    59761 aaagggttgc ggttgaaaga aggcatggtt atcacgatcg aacctatggt taataccgga
    59821 acttggaaaa tgaaaatgga tcctaacggc tggactgcgt atacaagaga cggcggactt
    59881 tcttgccaat atgaacatac attggctatt actaaagatg gtccaagaat tttgacatca
    59941 caaggagaag aaggcactta ttgatctatc cagtcaatag gtacgggaga ggtacatgaa
    60001 agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga cgactcaaaa
    60061 gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt atttactctt
    60121 gtctttattt ccattgatta ttgcttttgg taatatttta ccgtttctcc acatcgatca
    60181 agagactgta ctgacctatt tacgagaagt gattcctgag accgtttacc agtttatcgg
    60241 tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc ttgttatccg tttctgcatt
    60301 agcggcttta tggtctgcaa gtcaaagtat caatgctttg caaattgcga tgaataaagc
    60361 gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac taattgtcat
    60421 tgtcttgttc atgattgcta ttagtggggt aactatcgtt cttgggttgg gacagctgat
    60481 tttggatgct attcagccaa tcgtacaaat acccgtgaac tttatcgatc agttccaaac
    60541 tttgaagtgg ccaatcacat tggtagcttt atttgtcatc atgttcttga tttatttaat
    60601 tgtccctaat gctcagttga agttgaagac gatcattccg ggcacgatct tcgctactgt
    60661 cggctggatg ctgttatcac aagtgtttag tatctatgct aggtattttg caacgaggat
    60721 cagcggttat cagatcatag gaagttttat tgtactcatg ctttggctta attttgctgc
    60781 tacgatcatc attttgggcg gaatcatcaa tgcagttgtt caagaatacg tgacgggtca
    60841 tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc aataaattga
//
LOCUS       DAYYDS010000007        51531 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000007 DAYYDS010000000
VERSION     DAYYDS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.007"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1333
                     /locus_tag="ACHA02_000480"
     CDS             <1..1333
                     /locus_tag="ACHA02_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8668859.1"
                     /translation="NLLHVCKTTLLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIP
                     RCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCG
                     IEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYD
                     KENKEILSIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKE
                     EFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGN
                     QPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLN
                     PNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSS
                     ISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1425..1790)
                     /locus_tag="ACHA02_000481"
     CDS             complement(1425..1790)
                     /locus_tag="ACHA02_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8668860.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1787..2152)
                     /locus_tag="ACHA02_000482"
     CDS             complement(1787..2152)
                     /locus_tag="ACHA02_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8668861.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2344..2592)
                     /locus_tag="ACHA02_000483"
     CDS             complement(2344..2592)
                     /locus_tag="ACHA02_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668862.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2591..2719
                     /locus_tag="ACHA02_000484"
     CDS             2591..2719
                     /locus_tag="ACHA02_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8668863.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2776..4470
                     /locus_tag="ACHA02_000485"
     CDS             2776..4470
                     /locus_tag="ACHA02_000485"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8668864.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4570..4743
                     /locus_tag="ACHA02_000486"
     CDS             4570..4743
                     /locus_tag="ACHA02_000486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668865.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5121..6254
                     /locus_tag="ACHA02_000487"
     CDS             5121..6254
                     /locus_tag="ACHA02_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8668866.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6285..6605
                     /locus_tag="ACHA02_000488"
     CDS             6285..6605
                     /locus_tag="ACHA02_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8668867.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6634..7098)
                     /locus_tag="ACHA02_000489"
     CDS             complement(6634..7098)
                     /locus_tag="ACHA02_000489"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8668868.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7230..8483)
                     /locus_tag="ACHA02_000490"
     CDS             complement(7230..8483)
                     /locus_tag="ACHA02_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8668869.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8512..8829)
                     /locus_tag="ACHA02_000491"
     CDS             complement(8512..8829)
                     /locus_tag="ACHA02_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8668870.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8855..9175)
                     /locus_tag="ACHA02_000492"
     CDS             complement(8855..9175)
                     /locus_tag="ACHA02_000492"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8668871.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9639..11054)
                     /locus_tag="ACHA02_000493"
     CDS             complement(9639..11054)
                     /locus_tag="ACHA02_000493"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8668872.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11071..12387)
                     /locus_tag="ACHA02_000494"
     CDS             complement(11071..12387)
                     /locus_tag="ACHA02_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8668873.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12537..13235)
                     /locus_tag="ACHA02_000495"
     CDS             complement(12537..13235)
                     /locus_tag="ACHA02_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8668874.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13524..14513)
                     /locus_tag="ACHA02_000496"
     CDS             complement(13524..14513)
                     /locus_tag="ACHA02_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8668875.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14593..15426)
                     /gene="msrA"
                     /locus_tag="ACHA02_000497"
     CDS             complement(14593..15426)
                     /gene="msrA"
                     /locus_tag="ACHA02_000497"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8668876.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15601..17235)
                     /locus_tag="ACHA02_000498"
     CDS             complement(15601..17235)
                     /locus_tag="ACHA02_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8668877.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17222..18910)
                     /locus_tag="ACHA02_000499"
     CDS             complement(17222..18910)
                     /locus_tag="ACHA02_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668878.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            19056..20693
                     /locus_tag="ACHA02_000500"
     CDS             19056..20693
                     /locus_tag="ACHA02_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668879.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(20838..21578)
                     /gene="truA"
                     /locus_tag="ACHA02_000501"
     CDS             complement(20838..21578)
                     /gene="truA"
                     /locus_tag="ACHA02_000501"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8668880.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(21769..22404)
                     /locus_tag="ACHA02_000502"
     CDS             complement(21769..22404)
                     /locus_tag="ACHA02_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8668881.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(22551..23771)
                     /gene="sstT"
                     /locus_tag="ACHA02_000503"
     CDS             complement(22551..23771)
                     /gene="sstT"
                     /locus_tag="ACHA02_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8668882.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            23990..24988
                     /locus_tag="ACHA02_000504"
     CDS             23990..24988
                     /locus_tag="ACHA02_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668883.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            25184..27019
                     /locus_tag="ACHA02_000505"
     CDS             25184..27019
                     /locus_tag="ACHA02_000505"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8668884.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(27079..27795)
                     /locus_tag="ACHA02_000506"
     CDS             complement(27079..27795)
                     /locus_tag="ACHA02_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8668885.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(28022..28564)
                     /locus_tag="ACHA02_000507"
     CDS             complement(28022..28564)
                     /locus_tag="ACHA02_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8668886.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(28569..28796)
                     /locus_tag="ACHA02_000508"
     CDS             complement(28569..28796)
                     /locus_tag="ACHA02_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8668887.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(28875..30755)
                     /locus_tag="ACHA02_000509"
     CDS             complement(28875..30755)
                     /locus_tag="ACHA02_000509"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8668888.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(30944..31558)
                     /locus_tag="ACHA02_000510"
     CDS             complement(30944..31558)
                     /locus_tag="ACHA02_000510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8668889.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            31700..32260
                     /locus_tag="ACHA02_000511"
     CDS             31700..32260
                     /locus_tag="ACHA02_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8668890.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(32565..32879)
                     /locus_tag="ACHA02_000512"
                     /pseudo
     CDS             complement(32565..32879)
                     /locus_tag="ACHA02_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(33133..34768)
                     /locus_tag="ACHA02_000513"
                     /pseudo
     CDS             complement(33133..34768)
                     /locus_tag="ACHA02_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35323..35886)
                     /locus_tag="ACHA02_000514"
     CDS             complement(35323..35886)
                     /locus_tag="ACHA02_000514"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8668891.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(35901..36944)
                     /locus_tag="ACHA02_000515"
     CDS             complement(35901..36944)
                     /locus_tag="ACHA02_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668892.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(37077..37481)
                     /locus_tag="ACHA02_000516"
     CDS             complement(37077..37481)
                     /locus_tag="ACHA02_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8668893.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(37714..38349)
                     /locus_tag="ACHA02_000517"
     CDS             complement(37714..38349)
                     /locus_tag="ACHA02_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8668894.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(38615..39667)
                     /locus_tag="ACHA02_000518"
     CDS             complement(38615..39667)
                     /locus_tag="ACHA02_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8668895.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(39680..40618)
                     /locus_tag="ACHA02_000519"
     CDS             complement(39680..40618)
                     /locus_tag="ACHA02_000519"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8668896.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(41069..41719)
                     /locus_tag="ACHA02_000520"
     CDS             complement(41069..41719)
                     /locus_tag="ACHA02_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668897.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(41819..42328)
                     /locus_tag="ACHA02_000521"
     CDS             complement(41819..42328)
                     /locus_tag="ACHA02_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFM8668898.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(42529..43398)
                     /gene="scrK"
                     /locus_tag="ACHA02_000522"
     CDS             complement(42529..43398)
                     /gene="scrK"
                     /locus_tag="ACHA02_000522"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8668899.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(43418..44539)
                     /locus_tag="ACHA02_000523"
     CDS             complement(43418..44539)
                     /locus_tag="ACHA02_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8668900.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(44541..44936)
                     /locus_tag="ACHA02_000524"
     CDS             complement(44541..44936)
                     /locus_tag="ACHA02_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8668901.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(44955..45752)
                     /locus_tag="ACHA02_000525"
     CDS             complement(44955..45752)
                     /locus_tag="ACHA02_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8668902.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(45754..46542)
                     /locus_tag="ACHA02_000526"
     CDS             complement(45754..46542)
                     /locus_tag="ACHA02_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8668903.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(46556..47032)
                     /locus_tag="ACHA02_000527"
     CDS             complement(46556..47032)
                     /locus_tag="ACHA02_000527"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8668904.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(47104..49761)
                     /locus_tag="ACHA02_000528"
     CDS             complement(47104..49761)
                     /locus_tag="ACHA02_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8668905.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(49825..50184)
                     /locus_tag="ACHA02_000529"
                     /pseudo
     CDS             complement(49825..50184)
                     /locus_tag="ACHA02_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(50362..50682)
                     /locus_tag="ACHA02_000530"
                     /pseudo
     CDS             complement(50362..50682)
                     /locus_tag="ACHA02_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(50798..>51531)
                     /locus_tag="ACHA02_000531"
     CDS             complement(50798..>51531)
                     /locus_tag="ACHA02_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668906.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLID
                     LKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDL
                     LIRWQVITNFLMELKKYSNTQTKFS"
BASE COUNT        16269 a        10175 c         8078 g        17009 t
ORIGIN      
        1 caatctcctg catgtttgca aaaccacttt actcaatgat atcaataaca taaaaaaaga
       61 atttgaagaa cagatcgctt atacacatcg ggaaaaggac cgttacactt tatatttttc
      121 agaacatata cctcgatgca agatcatgca gcaattgtct caaaattcgt tgtttttaaa
      181 aacttgtctg ctttatttgg aagaagatga acctgattat cttcaattaa ctgaatgtga
      241 attcatctct gtcagcaaag cttattcatt aaaaaaacaa gtattagctt actttaatga
      301 ctgcgggatc gaaatcgacc gctactcccc tcgattcact gaaatggaaa gacgtctgct
      361 gcttcttaat gtgtcgtatc gtttaggcgg ttttaattct tgggagctac ctgaatcgtt
      421 ttttgaacgt gccgatcgtt tcattgaatc cgtaacggaa aatagtggca gattttatga
      481 taaagaaaat aaagaaatct tatcgattgg ttttgctatc agtttcctac gccagcaagt
      541 gtgtgctgtg acaatagatt caaaatttat cgaagaaatc aaaaaacgtc ctatttacaa
      601 ttatgtcgaa tcagcatggg aaaacactga tttccaaacc tattataaaa aagaagaatt
      661 cgcctttatt ttgacactat tcaatctttg taattatggt ttccattctt atcagttgat
      721 tgcggaagac tttcaacagc tacaccaagt gtttattgac aatactcccg aaataaaaga
      781 actagttgct acctttgaat ctcacttcaa tcaagaatta ttcggaaacc aaccgttcga
      841 acgtgcgctg attcatctga tgcgttcggc ttgggataac tatcaattat tcatgccaga
      901 aaaattttat cttttaaatg aggaacagac aaatttatat aaagaagtcc agacaatttt
      961 ttccagctgg tccagccagt taccttatga tctacggttg aatcctaatt gcatgcgggc
     1021 ttttgtgatc gaattgagcg gaattctccg tctgacaaaa gaacatctga ctatctatat
     1081 cgtcacaaat tcggatgttc attatctgat ttatcgggaa gccttggaag ctgtaactac
     1141 tttcgatttt caagttgcac caacaattta ttcaagtata tctgatataa agaagtatgc
     1201 gcagcagtcc tcaaaccggg tattatgtga acgtacatta tatacaccag atgcggtcca
     1261 atatgaaaat attattccta tttcgatcaa tacgatcgat cgagctatca tttcagctgt
     1321 gcaaaacaaa taaggggctg tgacaaaaat catgtcacag cctcttattt gttgaagatg
     1381 cccatttctg taacaatctc ttgatacacg tcaggctcac aacctcactt attttggaca
     1441 tctaggggtc cgatctcttt atttaaaaaa taagaaatga tggttcggat aactaccaac
     1501 gccgctagtt ggaaaatatc ctgcaaggta ggtttgataa tcgattcgat gatcccagct
     1561 gaaacaagaa tttccaaact tagaagtata tagcctccca acatcttttt aatagcattg
     1621 ttctcgctta ctcggtcgct ttgcactttt gtatggacaa ataaatattt caaaaaatcc
     1681 ttgattgata gacaaacacc ccaaaccaat aaacagatag caaagatatc aagtcccagt
     1741 acaagttttt cgaaatatgg aagaagaaac tgcatcaact ctgtcgtcat cctacctctc
     1801 ctcttctttg tttttctttg tcttctattt cctgattcaa gaaataagaa atgactgttc
     1861 gaatgataat aataagtgca agcttcaaaa tatctgtcca agtcggcttg ataactgact
     1921 cgatgatatc agcaacgact agtacttcca agcttaacaa aatataactt gctagcagtt
     1981 ttcttgtatc tgcattacga gtattacgtt ctgcatagtt tttcttgaca tgagaccaat
     2041 caaaaagttt gaatagaaca agaacgatcc cgacaattaa gataccgata gaacaaatat
     2101 ttaaaatagc aatgatccat tctagaaatt caattaaatt tgagcgaaac atatacgcta
     2161 cctactttct tttaatttgt tcctattctt ttattgtaaa agaaagttac cttgttcgtc
     2221 tatttaaaag ttgttctaat gataacggtt cgcttaaatt tttctttttc actctcccat
     2281 ttttctccct ccatccataa atcgtggaag tatccaaaac aacgtagcaa gttaaaataa
     2341 ggcttagtcc gtcatcagct tttcaaagaa gcactgtctc ggtgcttttt ttgtacccaa
     2401 atttctaaaa atagaaccat acacctcctc aaatcttttc ttcttaccta ccctattttt
     2461 atttacagga aaatcagaca cttcttcttt tttgtcaaaa tttccttttt cttctcgtcc
     2521 tatcatttgg cattctaata aaaacgaact atccttggat atagaaagag aaaacaagga
     2581 ggcgtttttc atgagtaaaa aattaatcaa agtcggcatc ggtttaggtt tgcttgccct
     2641 tggcgctgca tatctaggaa aaaagacagg actattcgaa gatgacagtc atttatatga
     2701 tgaattcgaa tcaatctagc atcctattta gttgaatcac tacggacata cttggaagac
     2761 attggaggaa tcacaatgag aaaaagtaaa gcaataatga ttggtgccgg tattgccaat
     2821 atggcagcgg ctgtttattt gattcaagaa ggcaaatgga aaggggatca gatcaccttt
     2881 tattcattgg atgatcacgg ttcaaatgat ggtgcaccga ccgacactgt agtggatgaa
     2941 tattggaata aaaaccatcc attagaaaac caaaaaggct atattgctcg aggcggtcgc
     3001 atgcttaatt accgtaccta tgtagatttg atggatctgt taagtcgaat accttctgtt
     3061 actgagccag ggatgactgc tgaagaagat acgagagatt ttgatgcaaa acaccgaacc
     3121 tttgacaaag cacgtttgat ggaaggtggc aaaggaatca ttgatggcgg aaaactggga
     3181 ttcaataata aagaccgtat tcttctgact aaactgattg ctatgcctga ttcagaagaa
     3241 gaaaaactag acaatatcac tatcgaagaa tatttcaaag atgatccaca cttttttgaa
     3301 acaaacttct ggtttatgtg ggaaacaaca tttgctttca gaacacgtag ttctgctcaa
     3361 gaattgcgtc gctatatgca tcaaatgatt tatgaattca ctcaaatcga acatctagtg
     3421 ggcgtcaatc gaacacgtta caatcaatac gaaagtatta tgcttccttt gatcgagtac
     3481 ttgaaagaac aaggatgcaa aatcattttg aaccgccgtg tcactgactg ggaattcaaa
     3541 gaaacaccga tgcaagatga aatcactgtg actggtttga aaattaccaa tacactgact
     3601 caagaagaag aatacgttgt ggtcgatgaa gatacagcag ttattttcac taatgggtcc
     3661 attacagatt cagctacttt aggagattac gaaacaccag ctgtagaaaa tatggattat
     3721 ggtgcagctt ctagtctctg gaaaaaagcc agtgaacgtt tctacaactt gggaaatcct
     3781 gacaaattct ttgctgatcg agatgcaagc gaatgggtca gctttaccct gacgacgaaa
     3841 aaccatttgt tgttgaatga aatcacccgt atcacgactc aagaaccagg aaatgccttg
     3901 aattcattta tttcaaccac accgatcacg ccgttaggcc aaaaagatgt caatatgtct
     3961 attgtcgtgc atcatcaacc gcattttacg tctcagaaac caaatgaaac agttatttgg
     4021 ggttatttcc tttatccaag acgacgtggc gaatttgtgg ataaacagta tattaaaatg
     4081 aatggaaaag aaatgctaga agaactgatt ggacaacttt caaaagtaga tcctggacca
     4141 gtgaatattc gtgaaaaaga aacagaaatt ttcgacagcg taataaataa tatccctgtc
     4201 tatatgcctt atgcttccgc gttgttcaat aatcgtgcca aaagcgatcg accaaaagtc
     4261 attcctaaac attcgaccaa tctggccttt acaggagaat ttgtcgagca gccttatcag
     4321 atgatcttta ctgaacaaag tgctgtacgt tctggagaaa tcgctgcttt ccattttgcg
     4381 ggtattccta tgtcccgtct tgtcaaaaca ccgcgttacg acaaagatat tcctactcta
     4441 gctcgtgccg caaaaaaaat gtttgaataa tcaagaggtt gtgaaaaagc tgccctgcat
     4501 caagtaataa gaacaaccag gctaaaacag cttcttatgt ttttgatttg gttggactta
     4561 ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa agctgccctg
     4621 catcaagtaa taagagcagc caagctaaaa cagcttctta tgtttttgac ttggttagac
     4681 ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttatga aaaaggcgtt
     4741 tagtttcgag tatgaagaaa aattttgtga aaatggttcc tcacgttttt gcataaattt
     4801 gacttaatac cgagaaactg atcccttctg tcataacctc tttttattta tgtctgtttc
     4861 tttttgaaaa caactatttt ttcaacgttt actctcttgc caatccaaca tcatctaacg
     4921 gatacaacta tttcaatgat taccattaca ctaaaaaatc ggaaacacac ctctccgaac
     4981 acaaatacat aagactatag aatgtttcat ttaagaaaaa aagcgtttca ttatcaaaaa
     5041 ggtaagatct cctttaaaat gaaattatct gtagtactaa taatgcagac aattttatga
     5101 atctgaaagg agcatctcca atgaaagcag tcacttggca aggaaaacag aaaatggaaa
     5161 tcagaaacgt agatgatcca aaaatattag aaccaacaga tgcaatcatc cgtatcacag
     5221 caacagccat ttgcggctcc gatctgcatc tttaccatca tggtgaagca gttttggaaa
     5281 aagggtatgt tgtaggacac gaaccaatgg gaatcgttga agaagtggga tctcaagttc
     5341 aaacattgaa aaaagggcaa cgtgtggtta tcccttttaa tattagttgt ggtcattgtc
     5401 atttctgcct gaatcacatg gaaagccaat gtgatgaatc aaatccagat cagttatatg
     5461 gcgggctttt cggatttggc aagttgaacg gtaatcattg gggtggtcaa gcggaatatc
     5521 taagagttcc ttatgcagac tctacttcat ttgttgtacc agataatgat cttcctgatg
     5581 aaaaagtttt atttctatca gatattttgc ccacagctta ttggagcgtt gaaaacgcag
     5641 gagtaaaaaa aggcgataca gttgtgattc taggttctgg tccagtcggt ctattcgctc
     5701 aaaaattcgc tgttatggct ggagcagagc gcgtaatcgc agtagatcca gtacagcacc
     5761 gtctagataa agcggctaat tacaatgggg ttgaaactta tttcttagaa gacacggcac
     5821 aagctggaac agaattgtat gaactggcta aaggtggcgc agatgtcatt atcgattgtg
     5881 taggaatgga cggattagag cctgtcaaag aaaaagcgaa aaatttagta agcttacaaa
     5941 gcggcacaat ctctccatta cagatggcat cgcaagctgt caaaaaattt ggaacagtcc
     6001 aagtaactgg tgtttatatg actccagctt cttcttaccc gctgcaagaa ttttttatgc
     6061 gggatatcgc tgtcaagcat ggacaggcac caattatcca tctaatgcca aaaatttacg
     6121 caatgattgc agaaggacta ttcgatccta cacaaatcat tactcattcg ctgccactat
     6181 cagaagcagc acaagcatat gagatctttg acaaaaaaga agataaaaat ataaaagttg
     6241 ttttgaaacc ttgaattcag taaaaaatac aggtgatttt ctttatgccc aaaatcaaac
     6301 gaaataccca tgagatcgaa ataggaaaag aagaagatat cgaaatacga ggacgtcgct
     6361 tccgcttcta tttccaaaat cgttacacat tgatctcact ggctgtcgat ttattaaccg
     6421 ggattttcta tatttttgga agccttgctt ccttaactaa aatccccgat cgatatggca
     6481 cctattttta tttggctggt gctattttct tgacgattcg tcctattctc agaatcgtaa
     6541 ggaacatttt tatttacgac gaaaaaaaag caatgaagag aacaagcaaa aaaaaagagg
     6601 catgagaatt tttcaaatct catgcctctt ttttcaccct atcgtatcac tttgatacag
     6661 acgagcggtc gtcccacttt cttccttgac cattgtcaaa aatgtccact cgtatcgttc
     6721 ataaaacgtg gtatgatctg taattaagta aagtttttcg tacccaagtt gatgcatttc
     6781 ttttctgatt tccgctaata tccgcttggc tatcccctgc ttacgctttt ccggttcaac
     6841 aaacagggca caaagatttg gtgtcagatc agggcggtta tggaagtcgt tttcaatgac
     6901 gcccgctcct ccgacgatgg tttctttttc atctaagacc acataccatt gaggaatact
     6961 ttccttatta gcgattccag tacgaatact ttcctcatac acaacagcag gaactcccca
     7021 ttttgatgaa aaccaattcg cgctttgttt tactaattct gggtgttctc ttaatttgat
     7081 ccagttatat gtaaccaatg tttaaacctc cgactaaaat aaaacgttta caaaaattat
     7141 acaagtcttt atgaaaatta gcaatgacaa caatagtgat gtaaaaaaaa aaacggaaaa
     7201 caatcacttg ttctccgttt tttgattaat caagcatcta aagcttcttt tcttctttct
     7261 tggatcgtag ctaccttgac gaacggtaga tagatcaaaa tagaaagaac caaattgaca
     7321 gcggctagta caccacctgc aatacttttt gttgccaata caccgccaat aattggtgga
     7381 gttacccatg gaggcataac ggtagctgct ggcactaaac ctgtagaagt cgcaaagtaa
     7441 gcaacactaa ctaagaccat tggtgtcaaa ataaatggaa tgaacatgat cgggttcaac
     7501 acgataggta acccaaacat cgttggttca ttaatattga agacacctgg cgcaatcgac
     7561 aagtttgtta caaccatata tggtttgttt ttacgtccta ctaaataaat agctaacagt
     7621 aaacctaaag ttgcacctgt tcctcctaag ttgacaaagc tgtcaaagaa aggtttgttc
     7681 acgataaacg gtgcgctatg tccagctgcg attgcattaa cgtttgcttc aatggctggc
     7741 gcattgattg tttgcatcaa aggatcgacc atattcgctc cgtgtaaacc aaagaaccat
     7801 aagaatggtg tgataaatgc aagcagtaag gcagatggat aagagtttgc aagtcccata
     7861 aatggttctt ggaccactgt atagaaagaa cctacgatat catcaacacc gaatgcagca
     7921 aaaattgcag caactaaagc aaacgcagag atagtgatca ttgctggtag taatgaagca
     7981 aatgcttttg caactgccgg tggcacacca tcaggcattt tgatgatcaa tttttcattt
     8041 ccgcttaaac gtacaaagat ttctgttgaa ataatcccaa caataatagc aataaacaaa
     8101 ccgtttgagc tcatacctaa tgctccgcct aaagcgaaga aagagcctaa agaaatcaca
     8161 cctgcggaaa caccgtcttt tcggtagccg ttagctaaat gataagcaag caagaaagca
     8221 atcaagacag aaagaatggc aaatgtaccg ttccaagcag cagccccgaa tcctttccat
     8281 gattcaccac caaaaattga gttcatcaat tcttggaacc cgggaatcgg taagttattg
     8341 atcattactg ctaatgctcc taagatcatt aacggcatac tcaccataaa tgagtcccga
     8401 atcgcaacta aatgccgttg tgcaccaatt tttgatgcta caggtataaa atgtttctcc
     8461 ataaatgcaa caaatttatc cataaatctt ccctcttttc tttactgtag attacgcgtt
     8521 caatcgacga tacagattaa tgatctcttt tgctaaatct gtaaaagcta tagatgtcat
     8581 cagatgatct tgactgtgga ccgtcaaaag cgtgacttca acgtgatttc cttgtgcttc
     8641 ttgtgtcagc atctctgttt gagagtgatg tgcttggact aaagattcct ctgcatcttt
     8701 gattttttca tctgctaaag tgaaatcacc ttttttcgca gcagcaatcg cttccattgc
     8761 atcgcttttt gcatttccgc caaacatgat cagacccatg atggcttcta gatttttttg
     8821 atcttccatt tattgtatcc tccgataatt tttattattt gtttattagt ttttctgctt
     8881 ggtcgagtac tttttcacca ttcatcatgc catagtcagc catattgatt acatctaaag
     8941 gaatgccttt aggtgctagc ttttcagcaa attgattttg cataaagcgt acttgcggtc
     9001 ctaaaagtaa aacgtctaca tttttagatt ctagttgatt atccgcatct gaagcagata
     9061 cagcaaagat gtctgcttcc attccttttg cttctgctgc cttttgcatt tttgtgacta
     9121 ggagacttgt actcattcca gctgaacata caagcatgat tgttttcttt tccattttaa
     9181 acacgttcct ttctatactt acacttgtat ataagcaagt agcgtgccaa ctttaaaagc
     9241 gctttaaaaa gttgatatca tcacatttaa ggtctatacc aagttgcata cacactaaaa
     9301 aagaaagcgc acactaaaca cacaaattaa tttttattac cttttttctt ttaacgtgca
     9361 caaaaaaagt tgtgacacaa aacttgtcac aacttttttc aaataaacgg tattcaaaag
     9421 cttgcatctg cggtaataag tccaaccaag ttaaaaacat aagatgttgt ttttactttg
     9481 ctattcttat tacttgatgc aggacagctt tttcacaacc tctcaataaa cggtgttcaa
     9541 tcagctgacc ctgggtatta gctcaaaacg ctggattcac aacctctttt atttcaaata
     9601 accagtaaat acaatgaaca tctacaaaaa acttctattt atttatagat ttcgtcaggc
     9661 aaatccaatt ctctcttatc aatcaaagtc tgataccacg aagcagattt tttcaatgtt
     9721 ctgcttcgtt catcgttcaa ttcgattcga actaatccat atcgattctt aaatgcattc
     9781 attggtgaca cacagtcagt gaatgcccat aacatatagc cttcgcaatt tgcgccttct
     9841 tctgttgctt tgatcaagta atgcaaatgc tgtccgataa attcgatacg gtaatcatct
     9901 tgcacgattc ctttctcatc catatactgt tcttcatatt ctcttcccat accgttttct
     9961 gttatcagcc aagggatatt atgataattt tcttttagat acatggctat atcatacatg
    10021 attttcggat agatttccca tcccctggaa ttattcattt tcttcccagg taaatcaaag
    10081 acttcataat atcgttcagg atggaaaggt gtctcttcat tccattgata acgaggggcg
    10141 cgaacgcgtt taggataata ttgattgatt ccaaggaaat ctactgtatg atgctgaatc
    10201 gtttctaaat cctctgttgc aggatcgaaa taaatcgatt gtttttgaca caactccagg
    10261 aactcttctg tgtagtatcc ttttaccatc ggatcaaaaa atactcgatt ataaaacaaa
    10321 tcatacatat aagcagcttt ttggtcttct ggagaattgg atcgagcata gaccatttca
    10381 ggatttaaaa tacagccaat ccttccacca taatttccgc tgcggtaaat ctctactgct
    10441 ttagccgttg ctaaaacttt atgataattc cacatcatcc acttttttgt gttttgttca
    10501 tgcggccagc gaacagcatc caaatagcaa cgcgtttggg gaacgattgg ttcgttgaat
    10561 gtaaaccagt tttttaccct atcaccgtaa cgatcaaagg caatttgagc atacttagca
    10621 taaaaatcaa ctacttttct tgaagaccag ccatcatatt tttccaaaag aaaagctggg
    10681 acttcataat gttccaaaca aatcatcggt tctacgtctg cttcgatcaa agagtcgatc
    10741 atttcactta tatgctgagc atattcttca tctactatac agttctcata atcagtaaaa
    10801 aaacgagccc aattgataga agtccgataa tgtgtcagat ggatggattt catcagatcc
    10861 acatcctgct ggtattgttc cataaaatta gttgctacgg ctgggccata cccattatgc
    10921 caaacaaaat gttcattttt ataccagcta tctataaacg aatcctgtcc ttcttttttt
    10981 cctttccaac cttccgtttg ccaagcagaa gatgcagcac caagaataaa attttttgga
    11041 atagtaattt tcatgatttc ctcctgtttt ttagatccca tcattttcca cacgatttgc
    11101 tacttttaca aatggcagat agatcaaaat agaaacaata atacaaacaa gttgcgtaat
    11161 aactgcaccg atatttcctg ctgttgccag ataagcattg acgatcggcg gcattggcca
    11221 agggaccatt acaactgctt tcgccgcaaa tcctaaagcg gttgcaaaat aaccaatcgt
    11281 tccagtgata agaggcgtta agataaacgg aatagctaaa attggattca gcatgatcgg
    11341 cataccaaaa ataactggtt cattgatgtt gaagatccca ggagcaaggg ataacttggt
    11401 gatttgtttc atatcttccc gttttccaac taacaatact gcaatcagta aaccgatcgt
    11461 gactccagac ccgcccatac tcatatacat atcccaaaat ggcatagtaa taatgttcgg
    11521 tacttctttt ccagcttcaa aagcttccgt atttacagca attgccgcta aaagcaacgg
    11581 ttcccgtatc ggcttcacca tttgatttcc atggatacca atcacccaaa agacttgcgc
    11641 aataaacatc aatagcaaaa tacccggtaa cccttgtacg acaccttcta atgggcgttg
    11701 tacgacattg taaatgatat cataaaggta cattcctgta agttctttga tgataaatcc
    11761 cgcagccgcg atacccgata cagtcagaat cgtcgggaat aaagcagaaa aactagcaga
    11821 aacattcgtt ggcacggtat caggcattct cagctgtagt ttcttctgtt tcctcaacca
    11881 gcaataaagt tcaatagaaa ggatggagac caacattccc aaaaataacc ctttcgtatc
    11941 tgtatattgt ttagccaata cgttttctac tataacagtc tgattttcat tcactaattc
    12001 ataaacagtt ggattaacag ataaataaga catcactgcg atgataccag ggaatacacc
    12061 atccacttta ttcagcttgg ctaattcgat ccctatcaga aaaacagcat agatggtcaa
    12121 gaaacttagt gtgacatagc tgatggcaga tgcgataggc tttaattctt ctaaaaaacg
    12181 caagaaactt atctgtgcta agccattttc cgagtcaaag accattgctg aaaacaatgt
    12241 gccaaaagca cccgtaataa ttaccggaat caatgcagca aatgcgtttt ttatcaccat
    12301 gatatacctt aaattattga ttctcgttgc cacgttccct aaaacattgg ctaatttatc
    12361 cataaaactc attccagatc ccctcattcc tgcttcttct ccttgacgtc atctcttttt
    12421 gtctattagt cagcgcttac atttcgatga acagaaacta tttactcctc ttttttacca
    12481 aatataaaac aaaaattcat ctgatgtatt tatcataaaa tacatcagat gaactgtcaa
    12541 ttagtttctt tttctcctat tattttctgt aattctctcc gatttattgc catatggaca
    12601 gtcattgcat caaacgcagc aagtgcattc cgcctttcga tggcctgaac gatctctgta
    12661 tgaatatcga tcgtctcgat tttcagatgc tggatatcat aatttttatt aaacaaagca
    12721 attgattcat taatgattgg gataatatgc agcaaggcta cgtttccgct agcttctgca
    12781 atcatcgtat gaaaatcgat gtctaattga acatgttctt gtgtgccttc tcgaaaagaa
    12841 gtttcaatct tttgcgttaa ttttttcatt tggacgattt gattcttcgt tgcatattcc
    12901 gctgccaaac ctgccatctt aggctccaat aaatatctca gttcaaataa gtctcgtatc
    12961 atccgatcct gatcttcaat caaagaaaaa ccgagtgggt cattggcaat ccctcgctta
    13021 tcactgacaa atgtccccgc tccttgccga acttcaagga tattcctgga agctagagcc
    13081 cgcacagctt ctcgcaccgt gcttcgcccc acctctaaat aatcggacaa ttcatattca
    13141 ttcggcagtt tatctcctac ttgcaactgc atttccttta aatatctaag gatcttgtca
    13201 gtagttactt ctactagggt tttattttct tccaaaccac atacctcctt ttcatctgat
    13261 gtattttttc gtaattatta taacacgttt gaatcatctg atgatttttt gtatacaaaa
    13321 ttgattaatc cgttcataga catacaaacg aagaagatat ttaccataaa aaagactagg
    13381 ataaaattca ctcctagcct tcgatcattt ttattttgaa cgacaaaaaa tatgatgttc
    13441 aataacagac gatcttcatt cgattcttga tgtcatcttt tgtttttttt aatatatgga
    13501 taaaaagttt gattacatcg tttctattct agaatttggt cgattctcaa ttcttcttct
    13561 ggtgagaaat ctaaattatc catcattcga acactttctt gtaactgact taaccgactt
    13621 gctccgacca gtacacttgt aacagatttt tgtcgcaaat tccatgcaag ggccatttgc
    13681 gctaatgatt gtcctcgtga tacggcgatt gtttgcagtg ctttgatttt ttccagagtt
    13741 gatccaactt gttcttctga aaggaatggt atttccttac gatgtgctct agaatcttca
    13801 ggaataccat gtagataacg atttgtcagc aacccttgag caagcggact gaacgtgatt
    13861 gcccctagcc cttcttcttc taatactgga gatagatcgt cttctagtag tgcacgagaa
    13921 aacatattgt agcgcatctg atgaataatg aacggtgctt cctttctttt taaaatttca
    13981 gtcatttttt ttgtatcttc tccattatag ttggatatac caatatataa tgcttttcct
    14041 tgtcttacaa gttgcatcaa agcttctgct gtttcttcca aaggtgtatc agggtctggg
    14101 cgatgatgat agaagatgtc aacataatct agccccattc tttggagact ttggtcacaa
    14161 cttgaaatga tggatttacg agagccccat tccccatatg gcccttccca catatgataa
    14221 cctgctttgc tagaaataat cagttcatca cggtaggctt gaaaatcttc tctaaaaata
    14281 cgcccaaagt ttttctctgc agctccagca gggcctccat aattattcgc aaggtcgaaa
    14341 tgggtaattc ccatgtcgaa tgcgcctctt aaaatctctc tttgattatg gataggatca
    14401 taatcaccaa aattttgcca taatccgaga gataaaatcg gtaatttcaa acctgaatga
    14461 ccggatttac gatattccat tttttcataa cgtgcactat ctgcaatata caatttttct
    14521 cctcctcatc aaatcttttt aaaacgcttt cttttttcta tttttagttt actacatttt
    14581 aaagaaaagg agttaatcaa aatgattttt tcgtatattt ttttgccatt tgttttttac
    14641 tcgttgatag atcagaaatt gattacgtgc ttgctggatt tttttataac gtttcggttg
    14701 ttttttgtaa aattgctgat gatagttttc tgctggccaa aacgtaccgg ctggttcgat
    14761 ttttgtgacg attggttggt ggaaagttcc tgaactgtct agtttctgtt ttgattgttc
    14821 cgctaattct ttttgtcgtt cgacttgata aaaaatcact ggacgatatt gcttccctct
    14881 atcttgaaat tgtccaaatg tatctgtggg atcagtaatt tgccaataga ttgtcagcaa
    14941 ctcgctataa cttatttctc ttgtgtcata aatgatctcc acagcttcca catgacctgt
    15001 gtaaccacca cttacttgat cataatttgg tttttcaaca tgtccgcccg tgtatcctga
    15061 caaaacagat aggatccctt ttttcgattc aaacggctcg accatacacc agaaacatcc
    15121 ccctgcaaat actgcacgtt cttgatagtc agcatcttgc atggcatgct tggaaaagtc
    15181 atatttttgc tccttatcaa atccttccgt aatcaataaa taaaagtcca ttacatcagg
    15241 cgtcagattc attcggatag ctaatggacg tagtgttgct tctagttttg ataactgttc
    15301 tttaacggat atattttctc ttatgtcatt ctttgtctga atcaatactt ttcgttccca
    15361 atctcgtgta ttcggattca aaatcagatt ataaagtgtt tgaatgaata tttgttcctc
    15421 atccatagat tctcctcctt tccgaagaag acaagctagt gactttagct gtaatttatc
    15481 ataaagtgag gaaataagcg attacttcat ccccctacag atttttcgta taaaaaagag
    15541 agaatcctta tcgacagatt ctccttttta atcaatttat tttataaatg aaaagttaat
    15601 ttacctttta tggaaaacta ttttaaaaaa taaacataaa tatgcagtta atgtaacaag
    15661 ataagaaata atagtcgtta ctgtataact tgtgcttact ttaatatcag caactccttt
    15721 gactccgctt atttgtacca atccattagc attcacctgc tcctttacag gttgatgatt
    15781 tatttctaca ttcgttccta aataagcata tacaggaagt gtgattactt ggctcttctc
    15841 attagttact ctatatatga tagaactacc agttgtttta taaggaaggt ttacccattg
    15901 actgccaatc tttgcttcgt gttttattat atgctcctta ttctccaatg aagcgatagg
    15961 agaataatca tataaaaaat aatccattcc tttagtggta tacgcctcta ctgtttcttt
    16021 gtttacaaca atatgtcccg gctgagaaat tattttttga ttgaaatttt ttttactaga
    16081 aacagttaaa gtgattaata acaaaacagt cataaataaa taaaatagtt ctcttgtctt
    16141 attgtatctt ctatttttga aaaatatcat cgttaatatc agagaagaga atattatttg
    16201 caagcttaag atccgccaag gaaattgtaa caatttggct ggcgtatttt gcagaagata
    16261 ccatggcacc aaactagttg tagctagcca agtcaaacac gtaccataat aaatcttttt
    16321 ttctatgttt gttaattttt taaataaaat caggggaata cataaagata acagaccaac
    16381 tagtcccatt gagtaagtag ttaagtcgtt gttcatagct tcagtcagac tatcgaacac
    16441 attagatgca tgagcatata aatttgtcac gctaggtttg ttgatttttt ggtagagcat
    16501 ttgttcaaac atcggatacc aaaaagcact cgtcagaacc aatgtcattc ctgccgcact
    16561 aaataatttt ttaaatatag caataaaatc tgcattcttg aaacctttag aaaccaaacg
    16621 aataaaaata aaaatgataa ttaatgctgt actcattaat gttgatagta catggctgta
    16681 cactagcaaa ctcatactca atgctagctt tacagacgga tacttttctt cttttccaga
    16741 gatgatttga taagcataaa ataatacagg gataacaagt gttattgcga tagcttcggc
    16801 aattgcggaa cgatagtaga tgtctactgt ccgataagca gagaaaatat agagacaact
    16861 aaataaaaat gccgcttttt tttgttttaa aaaccgctcg ccataatgat aagctacgag
    16921 tgttgtacac aagttcaata accaaacgta aataatatag cttacaatca aattttttgt
    16981 catccaatat aacattacag ctggaaaaaa agtcagaaag ggataaaaat aatttacacc
    17041 atatccagta taattgaatg tctcataatt aatcggactg gtaaaaatag tcgataaccc
    17101 cttgattcga gataagtgaa aagaaagatc tccagtggta taaatactct gtgtattttt
    17161 gaaaaagatg aaagaatata tactactaac tataattaat ccactcaata ttactacttt
    17221 tttatttatt ttcattcttc ttgtttatct cctttttagt cacatatcga gaatctttca
    17281 acttattagg tgcaaatcgt ttaaaacttg aatacaccac taaacaataa gtcagaatac
    17341 tgatccataa agccatgaga gcagttggag tatattttgt tgtaatttct atcttatttt
    17401 ctccagcatt tacatccaca ttcactaagc catgtttttt ataagagtca tttacgactt
    17461 taccgtttac ttttacctgt gtacctaaat aagcatatac tggtactgtc actttttgct
    17521 ttttgttgct tgccactgtg taagtaatgt tattatctgc agctttatac ttcccttttt
    17581 cccacgattc tcctactttt acttcatgtt tttctaagtg aggcctttct tttatcgctt
    17641 ctgttggggc gtaatcgtat aatcctccca tatctgatgt tgtataacgt gggagtgttt
    17701 cttcattgat aacaatatga ccgtgttttg attggataac ttttgcgtaa ttttcttttg
    17761 ctgctataga cgtgacgagt agaaacacca cagtagcgcc aatgattaac catgaatatt
    17821 ttttattcat agtctttttc ataaaaacaa gcgttatgat cattgaagaa aaaaagattt
    17881 ggatacctaa gatacgccaa ggaaactgga gtaagcttat cggtgttttt tgaaacaaaa
    17941 accatggaaa taaattagtt acgcataccc atgtagaaac tacgccaaca tagatgattt
    18001 tttctttttt ctccatttct ttcagtatga ataaaggaaa aatcaacgca accaatccta
    18061 ataaccccat agtgtatgtt gtaatatcgt tatttaacgc ttgaatgata ctatctgaaa
    18121 tagaaagtgc ttctagctgc aaatctgttt tgaagggact gtgtatttct tgataattca
    18181 tctgctgcaa cataggatac caaaaatagc tagttaatgc acaagtcaaa aaaacagcct
    18241 ttactaaatt cacgaccaca aattttatat cgttttttat ttgcttgctt aaaaacaaac
    18301 gaactataat catacaagca atcaatgcac tacacaatag tgtagttaag acatgagtat
    18361 acacaagtag acttatactg acagataaag cgatgacttt tttttctcta ttgaatatca
    18421 atccataagc ataatataaa acagctggga taatcgtaag agcgattgct tctgcaattg
    18481 cggatcgata atagatatca actgttcgat aagttgcaaa agtatataaa caactaaata
    18541 aaaaagcagc atctatcttt ttaaaaaact tttctccata ataaaaacta atcagaatcg
    18601 tacaaatatt taataaccaa atatatagaa cataacttaa tattaagtta tttgaaattc
    18661 cataaaaaac gactgcagga attattgtaa gaaaaggata aaaatagttc agtccatctc
    18721 cataattgtt aaatgttgta tagttgattg gcccttcaaa aatagaagat aaccctttga
    18781 ttcgtgaaag atgaaaagat aaatcacctg ttgtataaat ttcttgagca tttctcataa
    18841 aatttactga atataaagta ctaagtaaga taagaactac taaaagaaat gcttttttac
    18901 ttattttcat agatttgttg ttctcctttt ttaattctat tttcagtata ctagaattgt
    18961 ctttttttaa taagttttgc gttaaatttt tcatagcact agtatgatat actagaaaag
    19021 aatcaatttt tattactttt aaataggaga aaaaaatgaa aaaaaacaaa aaccaaataa
    19081 tagatatcat ttttatgtta tttctaagtt ttgcttacgt catccctcta ttcatgtcta
    19141 agggaatcta tcatagtgtc aatcaggata cttactttca tttatctcga atcataggac
    19201 tagataatgt atggagttct ccagtaaact tcaataactt cgatcatcat ggaacaatga
    19261 tgaatatttt ttatccatgg cttactcttt atccagcttt tttattttat aaaatgatgg
    19321 gaaatcttgt tttaagttat aatatctact atttttttat cactttttta acaatggtcg
    19381 tttcttattt ttctatgaaa caaataaaaa acaatcgtta tatttcgctg ttattttcga
    19441 ttatttatac attttccgcc tatcgtgcca ttgacatatt ccatcgtgct tcattgggag
    19501 aagctgtcgc tttaacattt ttgcctttga tactaatggg gtgttacgaa atttatatta
    19561 gagattacca aaaatggtac tggttgtcta ttggcatgac gctagtagtg tatactcatt
    19621 tactttctgt cgctatggtt tctgtattta ttggaggaac gttgtttctt tctttttatt
    19681 tttgggacca aaaaatcgct agactcttaa gtcttttaaa agctaccgtc ctcacctttt
    19741 ttttaagtgc gggattctta attcctttta ttcaacaatc tagagcacaa gaattgaaag
    19801 tcccactggg aaaagaatta agtggtatgg ctcctagtga tatgctgaca catattttaa
    19861 ataataacta taacaactat actattggat tattcttatt tctcggattg attggtgctt
    19921 ttatttttat aaaaaaatta actgctgatg atttatttat tttctttctt ggtgtatttg
    19981 ttcttttttg ttcaaccaat ttatttcctt ggcaactttt taaccataca ccagttaaga
    20041 gtctacaatt tgtctggcgg ttaaatggtt tttcttcttt atttatagcc tatacgatga
    20101 gtatagtgat ctattatatt ttttctacta aaaataattc atggaaaata atggtcttta
    20161 cgtttcttgc actaatattg catctctcag gagtaagcaa ttctatccat gctaacaaag
    20221 attcattaac acttatagca cctgaagacg ctgttgctat agctgaaaat tacaatcata
    20281 ctgattatgc aaacaaagag tcaatctatc atcctgatat gattaataat gatcaatatt
    20341 tactaggtaa ccaggaaata aatccaacaa ctcattttat gagtaacaaa cttacaattg
    20401 aattagataa ttcgaataat aaaaatactg tcttgacgac acctatttat cgttataaag
    20461 ggcaagtggc ttctattaat ggcaaactag tacaaacaaa attatctaag tttggcacaa
    20521 ctgaattaac tattcctcca ggtataaata aagtcgttat tacttatcag tacacaaaac
    20581 tagcaattgc ttcaagatat ttatctatcg ttactttgat tttattttta ctttatcgat
    20641 ttactttttc taaacaaaaa caaccgagaa gagaagtaca acattctcac tagattttta
    20701 tttaagaaag actttttagc tgtacacata aacaggctaa aaagtctttt tttatatttc
    20761 tttaatttgc ttcatacatt atttaacatc gatatttata ctgtgaaaca tgcgtttcat
    20821 ccattatcaa agtattttta tcgataataa acttcatcca aaaacagacc ttgtgcaggg
    20881 acggtttctc ctgcatgttg ccgcactttg ctttcaaata tttcatcgat tgtttctatc
    20941 ggcatggcac ctgtgccgat ttctagtaat gtccccatga taatccgcac cattttatat
    21001 aagaatccgt ctccgacaaa agtaaaatga agcatttctc cctttcgttc gatagataat
    21061 tcatcaatcg tacgcactgt ggattttttt gattttttca atgcagaaaa accaatgaaa
    21121 tcatgtgtac ccactaattt ttcgcaagct gcattcagct tgtccatatc caactcttga
    21181 gggaaataaa agctgtggtt tcgctcaaat gctgaaggaa tcacgttgtt ccaaacataa
    21241 tagctgtatt ttttgcccac cgcattatat cttgaatgga agcgctcagg catttcttcg
    21301 attcttttga tgacaatatc ccgcggaagg taacgattca taaagtcgat catttctgcc
    21361 agtccaattt ctgtgtttgt tttaaaattt gctacttgtc ctcgcgcatg ggtgccagca
    21421 tctgttcttc ctgatccaat aatttctatc tcttcatttg tcatctgatg caatactttt
    21481 tcgatttttc cttgaatggt tttatcgtca tctccaagtc tttgccatcc tgaatatctt
    21541 tttccgtcat attcgattgt caacttgatg tttctcatat tttagtggct cctctgtctt
    21601 ccatttggtc ttctctctac ctttttaaga ttgtagcaca aaatatagct ccttgcttct
    21661 ctaagacaga agctatacaa gaaaactgtg gaaatatata tttcagaaaa acaaaaaaca
    21721 gccaccggat gaagtatccg ctgactgttc aatttgacgg ctcaagtttt actgtttgag
    21781 atgcaagata ctcaacctgg caatgggcac ataattaaag aaaaaactga ttctttggca
    21841 aaaacctatt ttattcagta atggccaaga atttatagaa ggaattcttg ccagtccata
    21901 aatcgccgca aaaagcaaac tgatcacaga cagtccaagg tataagtggc ttacgtccat
    21961 ttgcccattt tttcgataga tcaagaaaag aagaactgga aacaataaaa aaccactgta
    22021 acctaaacct gatccgatat tatggaccca tgtcgagaca ttccacgaag attcatgggt
    22081 attgatactg aacaaacctg taaagataca atcacctata ccatagaacg cgatggctgc
    22141 tgtaactagg gtagctaaca cacgagatgt actaacaaat gcagcataca atgcaggcaa
    22201 tgacaggatg aaaaagatcc ctgaaaccac agaccaaata agaaacgctt ttcttacagg
    22261 actggaaacc tctccaaata cactgatcac gctcttcatt tgatctaatt caggataata
    22321 caatcctaat acgtacggag tcaaaaaatc actcagcacg cctaataata aaaagaaaaa
    22381 accaaattta cgtaaaaaat ccatgatacg cctccaataa caattaccta tgcttttaat
    22441 tgttattata aacgacttta tcttgattta acagaaacct ctatttacac gaacatctat
    22501 ataactcatt aaaaactcga atcattttaa aaatactaaa tatctaacgt tcaacgttca
    22561 cttaatccat ctaaacaatc tttcaatttg attttttccc cacttctttt acgcgcgcct
    22621 agctctccta ttgcagtaaa caacagatca cttgacgaat ttaaggcagt ttccattgaa
    22681 tcctggataa caccgataat aaatccaata ccgacgattt gcatcgcgac atcattagag
    22741 ataccaaaca aactacatgc taatggaatc aataacagtg aaccgcccgc aacacctgaa
    22801 gctccacaag cagctaaagc agacaaaata cataaaagga ctttcaaaac aaaaggtgct
    22861 tcaatcccta aagaatgtac agcagtcaac gtcatcaagg aaatggtaat cgcggcaccg
    22921 cccatattga tcgtagcacc taacgggatc gatatggaat aagactcttt tgtcaatttc
    22981 aatctttcag ccaacatcat gtttatcggg atatttgcgg cagaacttcg ggtaaagaaa
    23041 gcgggaagtg cgctttcttt caaacaaaag aaaatcaatg gatagggatt ttctttaata
    23101 aacaaaaacg tgaggaatgg gtacacaact aatgcgacaa acagcatcgt accgatcaaa
    23161 acaagtaaaa gctgcagata ctctgccaat ccagcgattc cagtcgtagc aattgattga
    23221 taaaccaatc ctaaaatccc tatcggagca aatgcaataa tcacttgtac gacttgtgac
    23281 aacgctatag atatctgatc caccaactct tttgtcgatt ctgaacgtgc tcgaaaagct
    23341 agtcccaaca atacagccca gaataagacg ccaaggtaat tcgcatcaat aagagattga
    23401 acaggatttt gtgtgacatt cgtcaacata gtagataaaa cactttccaa actttgcggt
    23461 gctttttcag atactgtttc ttgtaaagta ataggaacag taaaaattga acttacaata
    23521 actgctacaa gtgcagaaag caatgttccc actaaatata agatcaaaat aggcttcacg
    23581 aatgtcttat tcccaatttt atgcttggca attgacgcca tcgtcaagaa aaaaaccaga
    23641 ataggtgcga ttgccttcaa gcaactgaca aacaacgttc ccaatattga aataaatgtc
    23701 catgtgggga aaaagatacc aaacaatatt ccgactaaaa taccaacaac aatacgttga
    23761 actaacgaca tatccaaaaa cttctctaac aaatgaccac cctttcacaa ttttaacaat
    23821 cattgtatca ttttatgcaa agaaactaaa ggtataattt tctaaaaatt ctaacaatac
    23881 aaacaaccat ttcttgctaa ttttgtactt tttctgtcta cacgttgttt tttgagatat
    23941 tctaagttag gatgttatta atgggcaact tatcaggaaa cgttttctta tgtttttgtc
    24001 caattttttt gtgaggtgtt tattcttgaa aaaaataaag aaggagagtt atggcttcct
    24061 gacaatcttt gtcgcttgta taatgatggt tttacagcgt atattgattt cagcagtcac
    24121 tattcctaca tatgcaacaa accctggtcg attctacatc tatacatttt tacaaacttc
    24181 cgttgttatt ggtgcaaatt tgatcccact ttacatgggt taccaatatc aaaaaatcgc
    24241 ccgtttaaaa gttttacatt atttaagtcg ttttgccttt atattcctaa tagccacact
    24301 agtttgcaat atcttttttt acgtacaagc tggcagcttg aatattcgtg attattggct
    24361 tatcctaaca cctatcagtc agaattactt tacttatgct gtttcttgta ctttgctttt
    24421 ttgtagtatt ccttatacag ttggattttt aaataaatta tcgaaaacaa caattaaata
    24481 tctactatta tttttaacaa ttggattagt tggctgctct actctattta ataaagaccc
    24541 gtttgcatta cagaatggac agagtatttt atggattttt tatttatttt taatgggtta
    24601 tggtttgaaa gaatggaact ggaaagaaaa ggtccgctat aagcttcctc aagtatttat
    24661 cagcttgatt gttttattcg gtttgattat tttgatgaca caaatttcac tcatcatcag
    24721 aggaaataca gatacagcgc tgaggttttc taaaccatat tctctcttcg caatgtatta
    24781 tactttctct tcgtttttat ttttagaaat gctatctgat aaattaggta taaaaggaag
    24841 aggaaatttt cttgctgtat ttttgattgt cactcaagtt atatgtaatt tccctttagt
    24901 ttcttatcgc gtatctacat ttatgaaacg tgaatttcca aactcaggga aggcatggct
    24961 gataaatatc gggcagtttt ttatttgata tttattagca gtgtccctcc tagttatctt
    25021 tttattttat atacaaaaaa tccgtttcat tcatcgtttc atcgaatact tcgcttttga
    25081 ttcgattgaa caattaactc agcgttggaa aaaaagaatt cattggcttt ttgttcataa
    25141 aagttatttt ttagttgcag cattttttta ctgctttacc ttcgtgcaga ttttcgtttt
    25201 agaaccaaaa gaaggttgga aagaaactat ccaggttgcc ttaaaaatat ttactcagcg
    25261 acaagcaccc atgatcctga caatcatcat tattatggga tttttcttct tactattgtt
    25321 gatttccaat cgtttttggt acgccttgac tgctaccttg atcattaact tactgctcac
    25381 tatttcgaca gtaatcaaaa tggaaatgcg ggaagagcct gtctttcctt cagatttaaa
    25441 aatgctgacc ggattgtcag aactattatc gatggttagc ccagtcttat taatcgtggg
    25501 cggattgatc cttctcctat tattgattac tagtattatt gtacaacgta gactacaaaa
    25561 acagtattcc ttgaagattc attggaaacg tcgatttatc agtatcgctg tattattagg
    25621 atgtttttcg ggtgtctttt tcattaatca caaaaattcg ccttctttct tgctgtttaa
    25681 tttatttaaa gtaaacaaga ctttctttaa ccagcaaggc gccgtaaaag ataacggacc
    25741 agtcattcaa ttcctaaaca atatcgacat taaaatcatg gaagaacctg ctggctattc
    25801 gaaagaaaaa attaatggaa taatgaaaaa gtacaacaca aaggcagatg aaatcaacca
    25861 aacaagaagt gactggttaa acaatcagac gatcgttctc aatttaagtg aaagcttcag
    25921 tgatcctgca cgtgtcccta atctgacagt acctacagac cctattcctt acatcaaaag
    25981 cattataaca gaaggaacag gaggattgat gttatccgtc ggttatggtg gcggaacagc
    26041 taacatggaa tgggaaggac tgacaggctt gagtatcagt aacctgtctg cttcgttagt
    26101 tacaccttat acccagctag tggagcgtca gaaaataagt ccaaatatta ccgatctatt
    26161 cgatgaaaaa atagccatcc atccttttac agctgctctt tatcgcagaa aacaagtgtt
    26221 tgataaattt ggttttgaca ggttctatta tttagacagt ccggataaat taacttatac
    26281 ggataaaata gaacaaagcc cacgcatatc ggatgattca gcatacaaag aaaccttgaa
    26341 agctttacaa agcaatacgg aaacgtcaca attcatccaa ttatccacta tgcaaaacca
    26401 tatgccttat gatgattact atagtgaatt gaactacact gctgaaggaa ctgctgtcat
    26461 tcctaataga agaaaagaat tacaaactta tatgcaaggc cttcactata ccgatacagc
    26521 agtaaaaaaa ttcatccaag aaatcgacaa aatcgataaa ccgattacct ttgtttttta
    26581 tggtgaccat ttacccgcaa tctatagtgg gaatgattcg aaaaaatatg gattagaaca
    26641 gcaccagaca gattatttca tttataacaa caagtatagt cgtgaacaag cagctacgtt
    26701 aaataaagaa gttgttgctc ctaataactt tggtgctatg gctcttgagc atgcgaatat
    26761 caaagtaact ccttactatg cgttgttgac agaagtatct aatcatctac ctgcattaac
    26821 aattgatcct acagaaagct tgtctaatcg ttttaatgga aaacaggtct ttgttacaga
    26881 ggatagtcaa attatccaag aaaaaaattt aactgagaaa caaaaagaaa ttctagaaga
    26941 ttatcgtctg atacaatatg atcttgttgc aggtaaccag tacagtgcaa catgggcgac
    27001 acaaaaaata aaagactaac aaataaactg agcaaggggt tgtgataaaa gacatgtcac
    27061 aaccccttgt ttatctcatt aatttgcttg tccataaacg atttgattaa agccattttt
    27121 caattcatta ttcgctgtct cgttgaagta aagataatta tcgggattcc cctgattttg
    27181 ttgtgtcacc aatacttttg gctgattttg ctgaaacccg aatagataga cgtgcttttt
    27241 cagatatggt tctgtctctg catcggaata gacggctgtg atttgatagt ctgcttgacc
    27301 tgtcccactt tctgaccatt ctgcagaaac aggcactcca ccaatagcca tcttccactc
    27361 cccgttcaaa atagcttgag ggactttaag accatataag tccacactca tttgattcgt
    27421 ataacttttg tattgttgtc ccattgtttg gccccatttg gtcataaatg cctctaattg
    27481 tgcggatttg tctgcattcc acagtgtttg ttcaacttgt tcgctgcttt ctgaagagga
    27541 agatgaagcc tctacagaag aacttgaagt agatgtttga gttgtttcag tcgttttgga
    27601 agactctgac gaagtagagg tatctgctgt ttctgtactt gctgcagttg acttgctctc
    27661 tgtgctagtt gttgtagtag ttgcaactgt tgctttcgct gtttcttttg ttgattcttt
    27721 tttcgattca ttcccgttgg tacaaccagc taacaaaaaa gctagaccaa ttgttactaa
    27781 aatccttttt ttcaattaaa aaactccttt cgattactcc cacttcttta ttctatcaaa
    27841 taagcaatgt gattttattg atttttcgta ttttctcatc aatctttcaa atcttctaaa
    27901 aactgagaag cacccgctga tgttgtctgt aatacatcag cgggtgcttc ttttatttta
    27961 atcttcgtga ttttccaatt tacctcacgc tttatatttg tatttgaaga aacatagact
    28021 tttagtcatc atcgtcttcc tcatccaaat cttgtcggac agtttttcct tggagtcttt
    28081 gtacttggta gatgatttct ttattgaaac ttaaaagttc aacaagcaat tgttgcaagc
    28141 catatttttc ttctttttca gctaatttga ttgcccgtgt gataaagaca ttttggaaat
    28201 caaaatcttt tgcaaagttt tccaacattg ttgaggctgg taaatatttg ttttgaccat
    28261 gttcgtaaat ttttccataa cgcatgactt cttctaaagt agacggaacg atttcatttt
    28321 caatcactaa ctcatgagag attttattga accatctcgc ttcttgatca gcatattctg
    28381 aaaaaagggt acttaattct tgcgaagatg tacccaaaac atacaattgt gcttggatca
    28441 atttcatttc ttctaattta aaatttgcta atacatgccc caccatggcg ccagcagttg
    28501 gtttatgatg gtcgatatct gcttgttttg tttcttgaac aaatttttct tccactatat
    28561 tcattgattt acgcctcttt tacttttgct gattggactt cataacctaa tttttccaca
    28621 actgcagcta attgttcagg gtggacttca tctggatcaa agtttgcttt gattttgctc
    28681 gcattaaaca aaactttcgt attgcttacc ccaggctgtt gagaaaccgc atgttcgatt
    28741 ttttgcatac agcttggaca agctagtgta tctaattgca taataatttt tttcatcgta
    28801 gttcttcctt cctttttctt gatagtctta ttctagatta cagcttccat aaaaaaattg
    28861 attcttatca agttttacga tttttcttct ttgctttcgt gtccaaaata caataaacgc
    28921 atcgcgttaa agataacgat caagatacta gcttcgtgaa cgaacatccc actagccatg
    28981 taaataaacc cggcaaataa gccgttgagt aaaaataaga cgacaccaat ggcaatcact
    29041 acattttcac gggtatttaa aacagttttc ttagccaagc ggtaggcatg aaccaacgat
    29101 tcaaaattag attgcatcaa gacaacgtca ctcgtttcga tagcaacatc cgttcctgat
    29161 cccatagcaa tcccgatatc agccgcagcc aaagagggag aatcattgat tccatctcca
    29221 ataaaagcaa cgcgcaatcc ttgttcttgg tattttttca caaattgaac cttttcgtct
    29281 ggtaataatt cagcatggac ttcatcaatg cctaacatat cagctacata atcagcagtc
    29341 atctgattat cacctgtcag catcactaac tgttttgctc ctgcttgttt caacttagct
    29401 aattgttcag cgacttctgg tcgaatcaca tctgacacac ctatgatttg agtgattttt
    29461 ttgtcgattg ctacaataat gaccgagctg cctgtttgtt gtaattgatg taaatcttgt
    29521 acttgctttg gtgtgagaga aagtttgtga gcggcaatca atttggtgtt gcccgcacaa
    29581 actgtatgac catcaatctc agctgtgatt ccttgtccct tgattgtctg attatctttg
    29641 actgctaatt gttgaaaatc aatgtctttc tcctgagcat aatggatcac ggcgcgcccc
    29701 agaggatgat cggataacgt ttctaatcga gccactaaag ctaagctgtc cccttcatcc
    29761 tctgtatagt tcttgatagt ggatacagca gtagttcctt tggtcaatgt accagtttta
    29821 tcaaagacaa aagtatcgat tttggaaaaa gtatccattg cttcaccgcc tttgactaag
    29881 atgccccgtt tcgctccatt accgattcct gctacattcg aaacaggcgc accaatgact
    29941 aaagcgccag gacaaccaag aaccaaaaca gtgattgcta aacgaaaatc acgactaatc
    30001 agaccaacaa ccaacgcaat aagtaaaacg gttggagtat aatactgaga aaatcgatca
    30061 atgaattttt ccgcatggga tttggtatct tgtgcttctt ctactaattc gataattttg
    30121 gcaaaggtcg tatcatctcc gaccttagtt gcttcaatcg tcacgtagcc atctgctaaa
    30181 atcgttccag agaagacttc atctccactg attttgacga cttgtcgtga ttcaccagtc
    30241 actgcggctt catccgcata ccctttcccg tcaatgactc gtccgtccac aggaatagct
    30301 ccaccagttt ttactaagac gatatcgcct tcttccacgt catcgatttc cacttcttca
    30361 gtcgttccat cttcttggac aacactagca gtagatgggg ccatctcggt caaagccttg
    30421 attgactccc gcgttttggt caatgttttt tgttccagat agctaccaaa caaaaacaag
    30481 aatgtaacga tagctgactc cttgtattct ccgatcataa aggcaccaag cacagcaata
    30541 gtaacgagta aatcaattga gataactttg actttcactg cctgatacgc atggatcata
    30601 ataggtatga ccgcaataat agaagctatg gctagagcaa tgttatagaa caagacaaaa
    30661 ccagtaaatt ttccgataaa accaaaaata atcaacacag ctgataaggc ggtgatatga
    30721 tttcgatgct cttggatcca cttttgtaat ttcatctttc tccctctttt cctttcattc
    30781 ttacgagact aagtatagat acttatccat cataaaaaat tgaccactat caagtttagt
    30841 caaaaaggcg gttttctttt ggtagaatac actacaaaaa atagttagca agaagactca
    30901 ctaaaagtga tcttgccaac tattgtcgtt tctatcgaaa ttttcattga atgattttcc
    30961 tgtacagcat acatcggaga tctatttctt tatctaagat ttttatcttg ctaatttttt
    31021 cctttagtct ttcaaatcca cgtcgttcat agaattgata cgtacagcca ctatctgtaa
    31081 aaaggaatat ttcttttcct ttctctcttt tttcaaattc tgttagcaac ttggtaccga
    31141 tgccttttgt tttcaaatca ggatttgcag ctaaaaaaat gatctctcca tctggcgaat
    31201 gcttctttaa atatgctgca agcatttgtt catttgctgt ctcgtaagct cctttacttt
    31261 ctttagcaaa tatattttgt aaaaaatcga atatcttaac atatatttgc tgtccaaaag
    31321 aacgatgttt ctttttttct ccttttatct ccgccagcaa aacacctgcc aactcgtcct
    31381 ctgcatacaa agcaagaatt tgagttgctc gattcaactc catatacaaa aaatatcttc
    31441 cgtaaagatt cagcagaagt tcattttcca tatatctctc aaagttcatt ccttcaacag
    31501 caaaatagat agcttttttg taatctttct tttctaaggg tttaaaagtt agtttcatca
    31561 tttcgcctcc tcaacttcaa tgattgtgtt atacttacat tataatagac acagtgtcta
    31621 ctatctataa tcaatcatct ataagtttgt cgattaagcg acagctttgc tttgatatgt
    31681 cggataagga gataggtaaa tgaaaaagac agatagacgc gtcaaaaaaa cagaaaaagc
    31741 tttggctgaa acactatcca cattattagt aaataaaaag attcaagcaa tcactatacg
    31801 cgaactaaca gaaacggcag atgtacatcg ttcgactttt tatacacatt ataaggatat
    31861 ctatgatcta tatgatcaat tagaaagcaa cttttttcgg gatctaaatg aaattctctc
    31921 ttatgatcca gcccatagct atgaggaact atacactcgc ttgattgact atgtttacgc
    31981 aaatactttt ttatatcgct tatttatcct caaccatggt gatatttcgt ttcagaagaa
    32041 agttaaacaa gttattgaag aaaactatct gaacatatgg ctatatgaag acaagaaaac
    32101 gattatcaca gaagaaatga gattctttac tacgtaccac gtccaaggtt gtctatccgt
    32161 tctcaatcgg tgggtagaaa gcaattacaa gtactccaaa aaagaattat tagaactctt
    32221 acagaaactg aatgatcaca tagagaaaat catgccttga ataaaaacat actaagctta
    32281 gttaagaaga accgtaacct tctcgtataa caactgaatc ttatttagcc aaattgttta
    32341 ctgaagattt tttgagtaaa atttctataa ttcctactta ttgaaaagta gtttgtagtg
    32401 ctgcttctcc ctctctaagt gatatatctt gttgtaccaa tcaaattcac ctcatcatta
    32461 ataaattact tttaggctaa tttattacaa cttataggtt gaagctgtaa ccaatttata
    32521 gtcatattat atttttatca tccagtttat tgaattcttc tcgtttacct caattcattt
    32581 tcaaggggaa tcagctcatc atctaacatt ttacgccatg ctcgcttcat ttcttgtggg
    32641 tatttaaatg gtttcgcttc tggatgtttg gctttatatc cttcgtaatc cttactgttg
    32701 ataattgctt tcatattagg aattggatct tcacctggca tatattgcag attagaagca
    32761 tgtaattttt tctcagaaac aaaccactca ttggaaaaga catgaatcaa atcatcactt
    32821 acttgtgtaa aatagcgacg tttaatgaca aagatatctt cattgctatc tagtgtacct
    32881 tcatttcttt gtgcttaaat tcaaagataa ttttctcaat aaagattttt gattccagta
    32941 agattccttt actcctttta caacatagcg ttgtcctttg ttttgaaaca tcttcttccc
    33001 ctctttgcca ttaaaaatag cccaattctc tataaagaag attaagcttc attattataa
    33061 tataaagttt aaatttggtt tattacttac tttttcttat tctctagtaa gctaatattg
    33121 aaaaaaatgt aatcagatca ttatttttat atgttgtaac ttcctgataa gaaaactaat
    33181 taaaagaaag aggtaactac ataaactaat taaaaaagct aacttcatag ttggaggata
    33241 aatggaagaa acttcaatga ttgtttctcc ttgtgttcct tcaatcgaca ctagtcctat
    33301 tatcaatctc tcttttttgt ccatttactt ttgcgactgt tccccaataa tcaaatacag
    33361 gaagcgttat tttttgtcct ttagattcat gtactgtata gactattttc gaatccatca
    33421 cttgataagg catagtatgc cattccttgc caattcttac ttcatgattc tgcaaatgtt
    33481 ctttgtattg taatgcttcc gtgggtgcat agtcataaag tcctccggga aggagtgtcg
    33541 tgtaacgaat aagattttct tgattcatta ccaagtgatc tctctgagtg acaactttat
    33601 gttcatagtt tgcttttgtt gcagcagata cagccaacag taataaaaca attgccgtta
    33661 tattaactac ttttctttgc ttagctgatg tgctcttcat gaaaacgaac gataaaatca
    33721 acgagccaaa cagtacttga aagcctaaga ttcgccaagg aaattgaatc atttgaatgg
    33781 gtgtattttg taataaagac catggaaaaa atttggtgga taagagccaa gagactaccg
    33841 cactaaggta aatcactttt tctttgcgat taaatccttt aaaaaagaag agtggacaga
    33901 tcaaagaaat aataccaatg attcctaccg taaaggttgt caggtcatta ttcatcgcac
    33961 caatcaaact atccgagaca tttaatgctt ggtgttgtaa ggtcgtttta tctggctcat
    34021 taatcgattg aaccatgatt tgtcggaaca tcggatacca aaagtatgct gttaaaagaa
    34081 tcgtcattcc tactgccttt gacaactcca caatcaaccg gatgctttct ttttttcttg
    34141 tttcacttac tacaaagccg cctaagacta gtaagccaac ggctattaca ctcataaaag
    34201 tggaaagaac atgtgtataa atgagtaagc tcatactgac tgctaatgga attgcacatg
    34261 gctttttctc ataaatcagt gcgtaagcat aatacataac tatcggtata accgtaagtg
    34321 caatcgcttc agcaatagca gaacgatgat aaatatcaat cgtacggtac ccataaaatg
    34381 tatataaaca gctaaatata aaagccgcat cgattctttt taaaaatttt agtccataat
    34441 gaaacatcag catgatagta caaacattca atagccaaac atataagata taactaacga
    34501 ttaaattatt acttatccaa taaaaaatga ctgccggaaa aaaggtcaag tagggataaa
    34561 aataattcac tccgctccca taatgattaa acgtcgtaaa attgattgga ccagaaaaaa
    34621 ttgaagataa ccctttgact ctagcaaaat ggaaggcaat atcccccgat gtagaaatag
    34681 tatgaacatt tctaaaataa ttgattgaat aaagtccagc aataattgct aggagaatgg
    34741 acaatattat ttttttcttt acttgcatta cttaattata tactcctttc atggattgct
    34801 ttatctagta taacttaaat tttaaataaa tcgttattta tttttactct cacttaaaaa
    34861 agcactaaaa ttataagaga gggaggtaat ggtctgaaca ccaagaattg ttccaacatc
    34921 gaattgctta actaatgagt tcaaagctta attatgtggc tcttatattt accgagcgtg
    34981 gattgcgatc tctttagcga aacaaacatc tttcataaaa aaaacagcca tatccgcttt
    35041 cttggatatg actatttact actactttac tatctttcat tcttcttgtc tcacttatga
    35101 taaggcgctg cgtatcatga gtgaatgata ttaggaaaac ttcataaaat aaatctttta
    35161 tacatttcca cttctagatg gtcattcatg ttagaagaat caaactattt ttttactgtc
    35221 gtaataatat taaaacacta gaacaaatca attaaattcc ataaaatata aataatagac
    35281 ctagcatcat ctggtataaa atggatgcta ggtccgttat atttactcaa aattgattct
    35341 acgaatagat cttcttatca tttctatttt agtagtcatg cgttgttgtt cttctaaagg
    35401 tggtaatggt ataagtagtt tagataaatt tgtaccattt acatttggtt gcccagttcc
    35461 gtaagaaatt ttttctaata acttccaata taatggcgag tctaagaagc agtctacgta
    35521 ttctgataat aatttctcaa ctaacctaat tcgaatcaag taagaagcaa aaacagattc
    35581 ctcacttatt tccttaacca agaaactttt tcccatagtt cctcctgtcc ttgctatcaa
    35641 aatgtcattt tcttccaatc ttaaatcaac tagtttagaa ttactaatat cacagtaagg
    35701 cacactggac caatttacac gtccctcttg tatatctgtg attctcaaaa acttaacatt
    35761 gccttgtttt ttcgccgaat ctgtatagcc atattgaatc ttagtagaaa tacttcctaa
    35821 ataaacccat agtcgaacat cgctagatga tttttcttgt cgttctttat gaacaacagt
    35881 gatcatcttg agttgtccga ctatttttta gttgcagcaa tctttttggg acgcttattt
    35941 gcatgaagca acgttgctaa gtctagtaat aaaacattgg ctaacacagg attggctttc
    36001 tcgcgatacg ctgcggcgcc actttcaaac aatactgcta gacgatcttc tacaacaacg
    36061 atttgttcca aatacggtgg aaatttgatt cgttttagga tccggctagg agagtcccgc
    36121 tcggacacta agagttccga ggtataagga ccataagaac gactggctaa gagataatgg
    36181 ccataaaaag ccacaccttg aatcttatca gatgtttttc tgctcattgt cacggttggt
    36241 tggattttgt ctttgcggcg aattgctgtt ttcatttcag ctatcgtcgg caatagatag
    36301 aaagtcaact gtccttcacc ttttagtgtg aagttgccag ataccagttg atcattgttt
    36361 tgagtcaaat acgatgcttg tggaatctct gagagatcag ttgtcagcca ataagcgatt
    36421 ggctgtcccg tctcttcaga tgtatctgct aagatatcga tcaaacgtaa agcggctact
    36481 cgtccagtag cagatcctgt gattgtcaac cataaaagtt cccgttttgt atcatatact
    36541 aagcccccta catgtggccg tttaggtaaa atcagcagtt tgatcggctg tcctgtttct
    36601 ctatccagta catgaataat agaatgatgc tgatggtcat agcagtaggc agaaataaac
    36661 acaaaatctt gagaaaaagc caacccttgc ggcgtcatcc attgacaaac ctcattttcg
    36721 ccggtttccg gcaccaccat tcttgtttct gttaatccag gaatgttata ttgttttgct
    36781 tcagcctttt cccaagctgc ttgaacaata ggataccgtt ttaacttttt ttcaatatcc
    36841 tgatgtatcc gttgctgctt ccaatgctta aactttagga catagtaaac aagaaggcca
    36901 atgaccagca acactaaaat cattattgct acccgctgtg tcattttttt gcctccttgt
    36961 tctattcgat caatcgtcca tataccgcta ttaattaccg taactacatg tttcttttac
    37021 tagcgtttct ttataggatc tttcataatc tcatttatag taacattaag aatcccttat
    37081 tctactatag tcacttccac atcaatattg cctcttgttg ctttagagta tgggcaaaat
    37141 gcgtgggctt cttctgcgat tttctctgct tggtccttct caagtcctgg caaagaaact
    37201 tctaaaacga ccccgatttg gtagccccca tcttctaatt gaaataaaga tacccgtgct
    37261 ttgacttcag aatcagactc aagattattt tcttccagca tgtgttgaac cgcgccgttg
    37321 aagcaagatg cgtatccagc cgcaaaaagt tgttccggat ttgtcccttc actatggatt
    37381 cctgggcggt cgatcttcat gtgcatactt ccgtttggtg cctcaacttc gccatcacgg
    37441 cctccacgat tgatcattgc tgtttcgtac atttttttca ttgagatcat cctttctttc
    37501 gtcgttctat attaagtgta acgagtattt tttaatctga caaaggtaat gcttgcattc
    37561 ggcgacatca gttagacaac atcgatgatc agtgatagaa gcagtttatc tatacaaaaa
    37621 aagaagggtt ctatgatatt tggtgtcctt actcgccttg agtaacaaca ccaaatatca
    37681 tagaaccact taatcagctt cacactgctt tttctacttt cgtagttgaa aaaaacttaa
    37741 acaaatcatc ggcaaatagt taaagaaaaa acttaatcgt tgaaagaagc ccaattgctt
    37801 aaagaaggca aactgactaa tcgacggaat ccgtgctaat ccgtaaagac ctgcaattag
    37861 taaactaata actgttaaaa caagataaaa atggctaagc gtaccttgac cactttgacg
    37921 ataaagcaga acaagtaaca atgggaaaag tagaaagcct gcatagccta gcccagaacc
    37981 tatattatgg atccaagtcg atagattaca agagctttca ttcgtattga tactgaataa
    38041 gccagtaaaa atacaatcac ctatcccata taaaccaact gttgcaacaa caagtattgc
    38101 aagtgttttt gatgttccaa caaataggtg atacaacgca ggcaacgaaa gaacaaaaaa
    38161 aagtccagat acaactgacc agaccaagaa agcacgtctg actggactat ctacgtcacc
    38221 aaacacacta attacggtag tcatttgatt cagtttggga taaaaaaggc caaggatata
    38281 cggtgtcgat aaatcactca tcacacctaa taataaaaaa taaaaaccat atttttgcaa
    38341 aaaaatcatt ttcttcccct actttttagt gattattcga tacatccaac aatcaaattt
    38401 tttctcattt taacacatat ttttattcta gttgtctttt gaacaatcaa taacataaag
    38461 gtgagtttct tctacatttt aaaaaatcga atggcactga agagttcagt tgtcagcatc
    38521 aatctcctac tcaaaaaggc aaaaaaacgt aagtcagatg ttgttgacat ctcacttacg
    38581 catccattga ttgaaaattt cttagtaaaa tctactagcc taaaaatttg aagacttctt
    38641 ctcgatcata cagtttaaat actttctcgc ataaaaattg agtaattagt gccgcaaaaa
    38701 cagggatgac taaccaacaa agaacaacaa gaaagccatt tagccctgca tctaaagaag
    38761 ccaacggacc aacaagtccg accacgccga aaccagcaga agcaggcgta cctgaaatag
    38821 aaaatagagc gacaggaata gctgtaatca ctgcactaac taaacaagga ataagaatca
    38881 caggttttct aaatagatta ggcatcatca tcttcatagc gcctaaagca atcgcaattg
    38941 tcacacctga cttattaacc ttccaagagt tcactactaa aacaaaagtg gttgcagcta
    39001 cgcccatcgc agccgcacca gcagaaacac catctagttg gattgccatt ccgattgcta
    39061 ctgttgaaat aggagagata atcaaaaagg caaaggaaca accaatcaag atagacatta
    39121 acagcggttg taaggtagtg aagttattaa tcaatgatcc aattgcagaa gtaattgctg
    39181 ttacaaaagg gaataaataa aaaccaagta aacctgcacc tacacctaca acaatcggtg
    39241 ttaacacaat cgacactgac ccaaaactat ttccaatcag taaaatcagc ccgacagcaa
    39301 tcgatgctgt cagcatcgta ttaataatat cgcctgtacc agcaccaacg taaacacccg
    39361 tttcacccaa atcaggtgtg aatttgacta caccagaacc aacaaaagaa gctccggcta
    39421 caaccatcat tctcatcgga tcaaatccaa attgtaaggc aatcaaacca ccaatgatca
    39481 aaggtgttgc tagttgaaaa ataactgcta actgtgcgat cgttaccgca aagggatacg
    39541 cactaaaata ttttaaaatg gcacctaata ctgcattagg aatcaatcca ataatgattc
    39601 ctgttgctgt accagcgagg actttattca gaaaaatttt cggtgatatt ttttgtgaag
    39661 tttccattca atctctcctt tattttacgt tcaaactatc ttcttttgca tggacaagct
    39721 cagtcaagaa atcacaataa ggcgtcgcaa caccatattt cttccctttt ctagaaattg
    39781 cgccattgat ataatcgatt tctgttaatc gatggttctt aatcaagtct tgatacattg
    39841 aaggataatg taacccaatt gtttccgggt caaaacaaga ttcacaatgt gcaatgactt
    39901 ctgggacatc tagttcaatc ttctctactg ctgctacttt ggcaaactca ttgacaatcg
    39961 tcgccaccat tttatgagca gtcgatgttt ttcctagctc agccatatta acgtctaaga
    40021 ttgtgcatag tccgttcatt gttccgttca cacatgcttt gcgataaata gaatagtgaa
    40081 tattgtcaga aaaatgagca tttaatcctg attcagacaa tttgtctgct aatttttttg
    40141 cagctgcttc ttttccatca cctaaatttt gtaattctac tgaaccactt ccaaataatt
    40201 tgacctgacc aggaccttca agacctgccg tccacatggt atttccaata tagatatttt
    40261 ccattggtac aaatttctca ataatatctt catgcccgat accatttaat agacataaga
    40321 cctctgtatc ttttttgatt aatgattgga tatcctgcag cattttttcc agctgcatcg
    40381 ctttggtaaa tagaataatc agatcaactt gatcttcttt ttctacttcg gattgaagaa
    40441 cgattggtag ttttgcttct acctctttac cattaaaatt tgcttgtaat ccatgttctt
    40501 tgatttgttg gacatgttct gcccagccat cgattaataa tacttcgttc ccactttggt
    40561 gaagcatcaa cccaaagcga cttcccattg cgccagcgcc tgcaattgct attttcattg
    40621 attcttcctc ctttttcttt ctattatact catttgagaa tcttttcaca acctcgtttt
    40681 gttattcttc tcttatttat catgtttata tccaccattc aagttatttt atcagcaaat
    40741 caataaaaac tggaatagaa tcccaatttc atcgtaagag aaactagact agcaataaga
    40801 aaatagaaac attcatagaa aaaatctcac tcctctgatt ataaaagagg gggcattaac
    40861 ctcgtgtata ctaaaacctt ccattcattc gatggatgat tttctttttg tgaaaaaata
    40921 ttcattgtag tacgatgttg cgcaacacag acggatggta ttgcttatta tttattaaaa
    40981 tcgaacaata cacttattta aaaacttctt tcccagattt tcttccattt ttacagttat
    41041 aactagtatt atcatgaaaa atagcctttt attgtctatg ggaataataa tttaaaattg
    41101 gtactttttt attaattagt tcatcgccca ataatctctc cagtttctta gcatctcctg
    41161 actcaaccgg aattatccaa gcccagtcta aataattttt taaaatctca tcatattttg
    41221 caaatatttc attgccatat tccgtcctta aaattatatt ttcagtgcca gacaagtgat
    41281 ttatattgag ttgataaaaa agatctccat ctaaaaaaat atcattagaa gaagatttcc
    41341 ccacagtaat gaccattcca tcagctctat gtgttacata caaataattg tatgacctca
    41401 tttcacctaa tgaatcaatt atttcttgcc ctttagctct aatatattga tctctattac
    41461 ctatctcact cttatttaac aaaagttttt tcatgtcaaa tttaatcggc tgttttattt
    41521 ttgtaaattc gcttctacta ctaatccaat ccgagattaa tttttcaaca tgtcccagag
    41581 taaactctga tttattcgct aaatactttt tttgttgtat taaagacatc tttccccgaa
    41641 gctcaaaatt gagcctacat cgattctcta tctcttcaaa aagcatctta tcttcatcat
    41701 tcttatattc atcattcata aaaccaatcc agctcctcga ttaagataat attttaatct
    41761 catacaaaaa ctagctctca tcaaaacaac cattgttttg atttctcatg aaatttcttt
    41821 atacttttct ccgattataa attcctaacc ccttgagaaa attacgcata tatttatctc
    41881 cgcaaggctt aaaatttcgt tggccttctt ttctcaaaaa tgccccgatt tcgccttttg
    41941 atggatagat ttctgcctca gcaaaaacgt ctaaaatatc atcagtggtc atagacaagg
    42001 cgatttttaa tttttttagc aacacattgt tgatatattt gatttccttg cttttttgcg
    42061 gcgtgtctac tatcggctta tctttttgcg gaccacgcgc caagagaatc aggccattta
    42121 ggaatgcgtc aaaaacttgg ttgttgatgg ttttttcata cacattattt tctgcattat
    42181 ctttgttctt atcctgtact ttggtcagta ccgcctgagc ttcttcttta gtcagtggta
    42241 caccgcctag ctcaaaagct ttaatcaaat ccgtatcttt caaatcaatc gcataacgca
    42301 atcgcaataa cctatcattg ttattcattt gttcctccga agtatttagt ctttactttc
    42361 ttacgtactt tttgtcttgc taagattttt aggatcaatg ataaaaaccg catcacttat
    42421 gataaggcgc tgggtatcat gagtgaatga tattgctgaa tacaaatagt ccgaccacat
    42481 taagttgatc ggacttcata agcattttat tgactaatac ttttttgctt aagaattgat
    42541 tctaacatct tttgctaaag ttaagcatcc tatagttcct gcattatctg ctaacttagg
    42601 tgtgacaatg tattcttcga tatttggcgt atcgacatag ccgttaatta gttctaaaaa
    42661 acgtctttgt actttctttt tcagatgttc ttgttgcatg acaccaccac caaaaattat
    42721 aatatctggt gaaagcatca gggtcgtatt acaggcacat tgtgcaatat aatccgcctc
    42781 gatttcccag aacggatctt ctatagaaag atcttgtccc ttggttcccg tacgcatctc
    42841 gatagcgggt ccagatgcca acccttcgag acaatcatga tgaaaaggac aagtaccaga
    42901 gaaagtatct cctgaatgtc tcttaactaa cgtatgcccc atttctggat ggctaaaccc
    42961 ttcaacaaaa atcccatctt gtatagctcc tccaccaatt ccagttccaa cagtgtaata
    43021 aacaacactg gataaacctt ttccatgacc tgaaacatac tccccgtaac aagcagcatt
    43081 cacatcggtt gtccaggcaa ttggaatttc gaaatgctta ttcaacgttc caacaaaatc
    43141 aaaatttttc caggcagttt ttggagtaga ggtgatatgt ccataggtct ttgattcacg
    43201 atggatatca atcggtccaa atgaccctac tccaatgctt tctaattgtt ctttatagtg
    43261 attaaagaaa tcaattacca aactcattgt ttcattcggt gtagttgtag gaaaactcac
    43321 tctttctaaa acctttaaat catcattgcc aatggcacaa acaaatttag tcccacctgc
    43381 ttctattgag ccatacattt cttactccac cgtcctttta tctaggaatt cgttcttccc
    43441 tgtctaatag tttttcgaaa aactcatgtg gttccttttg gtaccaatag gcaacagaag
    43501 aaatatcgtc ttgacgctca taaagcttga tgtcatcatt tcctagggtt tggaaagtaa
    43561 cacgtaaatt ttctttgaaa ctaatggggt cgggtaaatg ccagcggtac aacccatgca
    43621 tcggtaaagc atcatcgcca aaaccatgaa ctggatttgg attaggtttt ccagcactaa
    43681 aataatcccg ggtgtgatct cttttagatt gaaaaggata gcccataaat agcgtattaa
    43741 aattttgagc atatgccctt tctccaaagt tctttttatg gaaagcccaa gcgccgccaa
    43801 aataatcttc tgctcctgta gatgttattg tagggaaatc ttcatctcca tctatataaa
    43861 acttaaattc tccttccccc caccagtatc tttccaatgc gcacaaagca aagtaggttc
    43921 ctacataata cccagtgcct ttgattccat caataagggt gtaatctttt ccttttgtcg
    43981 tgatgggttc acggttccat ttagcatgaa aatacatcgc atcatccggt acttcagtca
    44041 atgcataatt aaatgtataa aaaatgtgct taataaactc aggatgttca ttcgttaaag
    44101 taatcttagc tttttttcga aatggcatct caaaatagca attcatccct gatgttggat
    44161 tcactacgat tggtaaggaa ttcacttcgc atcttgtccc aaacccatta caaaaaaaat
    44221 ctcctaatgg actttctaca gaaggagtgg attcatcatc ccaataaata cgcaaaatta
    44281 aatcacgtaa gacgaaactc ccagcgattg ttttgtctgt cactgtcatc caaaaatgac
    44341 gtaaaattcc agggccttcc atttctgcta aagtaattgt ctctccagca tttatcggaa
    44401 cagaaggtga cccttttctc ccaattccta gatgactcgc tgtcattgcc gctttccctt
    44461 tttcaccatt tttattttct ggtgaaatag ttctactttc ctctcccttg tggagccata
    44521 tattatttaa acaattcatt ttattcgtcc tctatccttt cttcactttt ttgattattc
    44581 atatagatga tttgttgttg tgcctccgca ataattgctc caatatctaa attatcattt
    44641 cctaactgca aagcgattga cattgccaag ggaagattca ttcctgcaat tacgtgcact
    44701 ctgtcagaaa tataggtaaa aaacttttga tttacacttc ctccagccat gtcagttaaa
    44761 acaattattt cgtctgtagt atccactaac tctttaattt gttcatctat atcatcaatt
    44821 tcttcgacat atgcacataa gctactaatt tgattatgca tacctgttaa aaactctagt
    44881 gtctctttaa aaccctcagc tagtattgaa tggctagcaa tgatcaatct tctcataatt
    44941 caaatccttt tcctttacgc taatattcca aatgcagcca taaccatcgc aaaaacaata
    45001 acgagtagaa tcaaaatggt catactgatt tttttcttac tgagtagata atatgctgca
    45061 gcagtaataa ttactggaat taatgttggt aaaattttgt ccaacattgg ttggattttc
    45121 atactaactt caccaaaagt gaatttaagt ggcgttgaaa cactgataac tgaagcaatt
    45181 aatgatccaa ctactgttag ccccaatact gaagcagctt cagttaaata attaatcttt
    45241 tcaccgtatt cagtaacaat cttcattccc tgcttataac caaattcaaa gcctctcatt
    45301 cggaaaaaga agattaccat gattaaaatg aaccataaaa agattcctac aggattattt
    45361 tctaaagcca tataagccgc aattgatccc atgatggttg ggatcatcgt catcaaaaga
    45421 gagtccccaa taccagcaat tggacccatc gtaccaactt taaagtcatt aatcgcatct
    45481 tttgcttcta atccttgctt ttcctccatt gcaacacaag cacctaaaat aatggctgcc
    45541 agccaaggct gtgtattgta atactgaaag tgattattta aagcttcgat gtattcatca
    45601 tcatctttgt ataattttct taaaataggt gacaatgcgt aagtcacaga tgcaccttgc
    45661 tgtgtttgat aattaaaggt acaaactgac atcaaccaac gatagttcgc tttcctaaga
    45721 tcctttttcg ttacttgata tttttcgctc attttattca tcctccccaa taaacccaac
    45781 atcagaagct cccgcagaag cagtgatttg tgcatctttt tcagctctat gatacacatt
    45841 aaatgctaaa gcgaatccta tgatcgccgc tccaataata ggtaccttca aaaaagcggc
    45901 taagaaaaat ccaacaagta aataactaat gtatcgacct gtaggcataa agcgtaacaa
    45961 catcgcaata ccaacagcag gcaacatttt tccggctaat gttagcccat ccataaacca
    46021 attaggtaaa acatccaaaa cgtaattaac agcagactgt cctaaagtaa cggcaataaa
    46081 tactggaatt gctgcagata aaccaaagaa aattggcccc aaccacaaaa cactattcat
    46141 tttcttaaat tctcgtttat tcgcatattc ctgtgatttg cgaacaacaa aaccatttaa
    46201 gattttgaca attacatcta gttgcacccc taacatacct acaggtagtc ctattgctaa
    46261 tccggcttct actccaagct ggctaccaaa tgccgttgca ataattgcgc acgttccata
    46321 gtccggtaca gatgagccac caaaaccggc aactcctaat gacatcaatt gaactgtacc
    46381 gccaactatc aaacctgttt ttatatcacc catcaaaatt cctgtgaata atccccaaaa
    46441 aactgcgtaa tttacaaaga tcatcggaat cccataataa tcaacatgtt taataaatgc
    46501 taatatagtc agcataataa tttgaaaaat agtaaaatgc attatcctta ctcctttatc
    46561 ctaattttga ttgaatttcc tgtgccttat ccgccggaac aaattgtgta acaatttcta
    46621 caccagaatc caacaatttt ttatacgtgc tgatattttc atctgatgca taggctctag
    46681 aagatagctg taatgcatta tctttaacaa cagtaccgcc aacaattagt cggtcaattc
    46741 taataccgtt atctactaaa tgaaggattg tttccggctc tttcacgatg acaaatactt
    46801 tttcctgatc gtatttcccc tttttaaaat tcgcaatcgc tgtttcttca gtgataattg
    46861 atacagccat tccagcaggt ctagctaaac gcatacttga tttttggatg tcgttatttg
    46921 cgacagaatc atcgatcacc atcactctgt ttgctttaga cattggtgcc cattgcgtga
    46981 caataatccc atgcaataaa cggttatcaa ctctcgacat aacaatactc ataaatactt
    47041 cctcctttat ctttcatttt cttacaagca attaccatgc caaaacaaaa agaacttgat
    47101 aaatcaagct ctttttacat aatcataaac gtatttaacc tcatgaatcg ggatgtcgac
    47161 actataataa tgttcaacga cactgaaaga ttgcttaaaa tgtgtctgaa atttttgaaa
    47221 ttcctcactc gtttcattga aataaatttc atcttccaaa gtattttgtt ttactaatcg
    47281 ttcaattaag caactaatgt gaacatataa tccgaaacac gtattattat caaatgaaat
    47341 tcctagatca ttttgtaaga tgtatatagc atctgaaacc cgttctaata atttagtcgg
    47401 atttaatatg gtcaattgct ccattaaatt cgttagacta aaattcttca gaatattatt
    47461 gttcaaaact ttcagttcat tatcattaaa gtgtctattc aattgattgg agaatttact
    47521 tatattcatt cctaaaatca tatcttcaat tgaaaagaat tctacatctt ctagatgtgg
    47581 atttagtgtt ccaacaataa aaagaatatt gttctcttta cgaagctccg ataaatagtt
    47641 ttcattcact aaagagtaat agtcacacgt ttctatgaca agatcagtat cccttggaaa
    47701 gctatcttca agtatctttt ttaatttaaa cgaagttcct aatccgcttg cacaagaaca
    47761 gacaatcata tccttcttaa tatttgatga ttcataaata taatccacat tttgattttc
    47821 ttttacttgt tcaagaatct gtttaagcgg taaatctaat aatatctgat ttgcaacatc
    47881 aatagccatt cttgttgaaa cattgtttag tagtccgaat tttttttgag cgtctagttc
    47941 caaatttaga taaattttct ccagtgaacc catatcaaca agtaaaataa tttcttcata
    48001 tccggtacac tcttgtaagt atctattaat tttgtctgca atctcactac ttgaagtcat
    48061 tagtggcata tcaatggcgt cgtatatata tctctcaacg atcctattag cagtacttgc
    48121 tatacaggtt gccgttagat ctccatgagc caaaatcagt gctaaacgtt tttgctgaaa
    48181 ttctttacta cacattaact taaataaaat aaccaaaaca tttttttgaa tttttgttaa
    48241 ataaatatcc cctatttgaa taaaacgatt gactaattga acttctcgac tacaccaact
    48301 attaatatca ttgatgaagg aatataattt atcgtccgat gttaattgga tttgataaat
    48361 ccaagaattc aaaaagatag acgttatttg aagataataa tagtcaaatt ctatttgaaa
    48421 atctctctct atttccttaa catactgtct aaatcgaatc tcttgatagg tgggatatgt
    48481 taaagctgaa ttattttgga tatcaacatt cttttgtcta aaatatctat catatttctc
    48541 aaaaagctgc tgcttatcag tgtctttctt ccacgtttga taaagatact ctataagttc
    48601 gttgatatta aaaacttcgt ctactttttc caaatcatcc atttgaatat attttaattc
    48661 attctgtctt ttcataacct tcatgccgtt gaaattcacc cattgtggta aatgatacat
    48721 ctgaatataa agttcttcca aatcatctaa gaaataaaag gcattggcac aggtcaattg
    48781 aatgatattt ttcaaattgc caacattttc actaaagcga tagtttaata aaaaatctaa
    48841 taattgttga gatatcagta ttttctttgc tagtctttct tcttctgatt taaaaaatat
    48901 cttgattaac tgttttcttt cataaagaga acgactgtct aatgttggaa gtgttagctt
    48961 tacaggtatt cttctcaaca aagttgataa tagtttatcc tcaggtggtt ctgatgttgc
    49021 aaatataatc cagcaacttg atgtgtacca gttttcatta tcacccatcc gatgaaattt
    49081 cctcgtatcc ataaactgaa aaagtttctc ctgacattca ttgctcaatg catgaatttc
    49141 atctaaaaat aagactccat tattagctaa ttctgctaaa ccctttcttc catcttcggc
    49201 tcctgtatat gcacctttta catgcccaaa aaaattggac aagaaaagtt ctggattatt
    49261 ggcatattca ctacaattaa ctgaaatgaa agatgccttc tcttctatat aattattggc
    49321 caacaaatcc ttatacacat aatttgctaa atagcttttt ccagttccag tctcgccatg
    49381 aagtaaaatg ggtaagccat ttggcggata gtagattgct gctttaattt gagcaattac
    49441 atttcgcaga cttccttgac ttccgataac cctttctatt ccaaccctaa aagagctatc
    49501 ataccatttt tccgcttcct ctgaagagct aaaaatttgt tgatttggaa ctttctccgg
    49561 taaagaaaag aagattactg gacgagtatt gatttttcca aattctccat cttttacgaa
    49621 ttcattcaaa tattgagaga caatattacg actaacattt aatttttcag atattttctg
    49681 tgctgtattg ttcccccatt tatttttaat ctcagagttc tctatgacat aattgacaat
    49741 gcgttctttt aattcttcca tcacagcacc tcctcgacac tattcgaatt ttgccgacag
    49801 tatttttcca taaatatgac ttgctaaaaa ggctttgttt agatcattca attggaacgt
    49861 tgcttgtttt gcttgatccg ctaagacgtt gaaaagatac tctggcgaaa tcgcataccc
    49921 caaaatatca acgattgttg caatcagatc ttcttttgat tcttcatctg aaagtaattc
    49981 acgatacaac atcgtttgtt tgcttactgt gtcatattcc tctaaggatt catctgctaa
    50041 aagaaccact tgttccaaca aacggtcgga taaatattta tagaaaatca gccccaataa
    50101 ataattttta tattctgacg catccatttt actacggaga ttatctgccg cactaaataa
    50161 cttctgattt aattctgcac ccaaaaagat acactcctat ctcttattta aagttgctat
    50221 ataccctctt attttagcat actcagtttt agaaaaagca aaaaaataaa gaaacttatt
    50281 cttgataaca agctacgtga cgtatatccc taacacaaaa atagccatat cctttggttt
    50341 ggatacggct atttgatatt actcattcaa ttatcaaatc acagtaggtg tgaatctcat
    50401 cgggatcggc aatatctact tctgattagg atcaaaggtg attttctcaa taaaggtgtg
    50461 atacaaagct ttaacttgct tcttctcact ctggataaga aagcaatcca aactagtcaa
    50521 tatctgttgg acatctttga cttgaatcgg ttccccttgt tgttgaaaga tccctaagat
    50581 ttcattctct cgaatttgta actgtcttcg tttttcagtc aattcattta aacgatcttt
    50641 taacatcgga aacaattcgg gactatcttc caaagcagct tcctgttttt gctctaatag
    50701 ttagattttg cgtaccattt tcttctttta ttttccttcg attgctgatt ttaatatagt
    50761 ctaaaggaaa tttatattta ttgtattctt tataacttca agagaacttc gtttgagtat
    50821 tactgtattt ttttaattcc ataagaaaat tagttattac ctgccaccta atcagcaaat
    50881 ctgaacctga gtcaatataa ttaatattat tttccatctc caacattgta agtactgttg
    50941 gtgttaatat agggactttg tacaattgta tttttttgat tttatcatct gcacaaagga
    51001 gattttttaa atctaatcct tcttttattt caaaagttga attttttaaa tctataagag
    51061 taaaaccaat tccttcatac tgaatttctg aaaatcgaat ttttctccca gttgatagat
    51121 caaggggcag aaaacctttt attacattgt gagtgtcacc atcatttaca atgtcattag
    51181 gaattttgat tttagaattc tgtggagcac ctattggaag ataataattc attataattt
    51241 tgtagtcttc atgaggaaat aaagtacttt taggattgct aagatattct cgtaccgaat
    51301 ctaagaatgg cttatccatt gaatcttcat ccccatttaa actaaaaaac attcctacaa
    51361 ttgcttttgc aactctaagt gaattcattt tctttattga atgtgttaaa tcagacttac
    51421 cattctttaa acttgtattg ttataaagga aatttttatt gtcttctgca aatctaatat
    51481 attcctcaac atactcacta ccaaaaaaac tattacattt actacataac g
//
LOCUS       DAYYDS010000008        51399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000008 DAYYDS010000000
VERSION     DAYYDS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.008"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACHA02_000532"
     CDS             330..1175
                     /locus_tag="ACHA02_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8668907.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACHA02_000533"
     CDS             1456..2676
                     /locus_tag="ACHA02_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8668908.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACHA02_000534"
     CDS             2673..5393
                     /locus_tag="ACHA02_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8668909.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACHA02_000535"
     CDS             5377..6321
                     /locus_tag="ACHA02_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8668910.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="ACHA02_000536"
     CDS             complement(6390..7400)
                     /locus_tag="ACHA02_000536"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8668911.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="ACHA02_000537"
     CDS             complement(7580..7924)
                     /locus_tag="ACHA02_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8668912.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="ACHA02_000538"
     CDS             complement(7917..8351)
                     /locus_tag="ACHA02_000538"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8668913.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="ACHA02_000539"
     CDS             8489..9226
                     /locus_tag="ACHA02_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8668914.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="ACHA02_000540"
     CDS             9219..10430
                     /locus_tag="ACHA02_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8668915.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="ACHA02_000541"
     CDS             10634..11416
                     /locus_tag="ACHA02_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8668916.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="ACHA02_000542"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="ACHA02_000542"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8668917.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACHA02_000543"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACHA02_000543"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8668918.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="comGA"
                     /locus_tag="ACHA02_000544"
     CDS             14132..15061
                     /gene="comGA"
                     /locus_tag="ACHA02_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8668919.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /gene="comGB"
                     /locus_tag="ACHA02_000545"
     CDS             15018..16067
                     /gene="comGB"
                     /locus_tag="ACHA02_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8668920.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /gene="comGC"
                     /locus_tag="ACHA02_000546"
     CDS             16064..16342
                     /gene="comGC"
                     /locus_tag="ACHA02_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8668921.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /gene="comGD"
                     /locus_tag="ACHA02_000547"
     CDS             16317..16787
                     /gene="comGD"
                     /locus_tag="ACHA02_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8668922.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16759..17106
                     /locus_tag="ACHA02_000548"
     CDS             16759..17106
                     /locus_tag="ACHA02_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668923.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            17069..17494
                     /gene="comGF"
                     /locus_tag="ACHA02_000549"
     CDS             17069..17494
                     /gene="comGF"
                     /locus_tag="ACHA02_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8668924.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /gene="comGG"
                     /locus_tag="ACHA02_000550"
     CDS             17466..17825
                     /gene="comGG"
                     /locus_tag="ACHA02_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8668925.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="ACHA02_000551"
     CDS             17997..19004
                     /locus_tag="ACHA02_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8668926.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="ACHA02_000552"
     CDS             19029..20213
                     /locus_tag="ACHA02_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8668927.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACHA02_000553"
     CDS             complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACHA02_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8668928.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22338..22466)
                     /locus_tag="ACHA02_000554"
     CDS             complement(22338..22466)
                     /locus_tag="ACHA02_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668929.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22811..22954)
                     /locus_tag="ACHA02_000555"
     CDS             complement(22811..22954)
                     /locus_tag="ACHA02_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8668930.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="ACHA02_000556"
     CDS             complement(23176..23655)
                     /locus_tag="ACHA02_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8668931.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="ACHA02_000557"
     CDS             complement(23728..24012)
                     /locus_tag="ACHA02_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8668932.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="ACHA02_000558"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHA02_000558"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="ACHA02_000559"
     CDS             complement(24356..25642)
                     /locus_tag="ACHA02_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8668933.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="ACHA02_000560"
     CDS             25780..26451
                     /locus_tag="ACHA02_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8668934.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="ACHA02_000561"
     CDS             26417..26905
                     /locus_tag="ACHA02_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8668935.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="ACHA02_000562"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="ACHA02_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8668936.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="ACHA02_000563"
     CDS             27866..28630
                     /locus_tag="ACHA02_000563"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8668937.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="ACHA02_000564"
     CDS             28863..31076
                     /locus_tag="ACHA02_000564"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8668938.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /gene="dtd"
                     /locus_tag="ACHA02_000565"
     CDS             31087..31533
                     /gene="dtd"
                     /locus_tag="ACHA02_000565"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8668939.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="ACHA02_000566"
     CDS             31704..32963
                     /locus_tag="ACHA02_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8668940.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="ACHA02_000567"
     CDS             complement(33052..33999)
                     /locus_tag="ACHA02_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8668941.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /gene="hisS"
                     /locus_tag="ACHA02_000568"
     CDS             34393..35685
                     /gene="hisS"
                     /locus_tag="ACHA02_000568"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8668942.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="ACHA02_000569"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="ACHA02_000569"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8668943.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="ACHA02_000570"
     CDS             37578..38453
                     /locus_tag="ACHA02_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8668944.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="ACHA02_000571"
     CDS             38719..40602
                     /locus_tag="ACHA02_000571"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8668945.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="ACHA02_000572"
     CDS             complement(40677..40961)
                     /locus_tag="ACHA02_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8668946.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="ACHA02_000573"
     CDS             complement(40951..41685)
                     /locus_tag="ACHA02_000573"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8668947.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="ACHA02_000574"
     CDS             41814..42437
                     /locus_tag="ACHA02_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8668948.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="ACHA02_000575"
     CDS             42629..43750
                     /locus_tag="ACHA02_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8668949.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="ACHA02_000576"
     CDS             43757..46885
                     /locus_tag="ACHA02_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFM8668950.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="ACHA02_000577"
     CDS             46904..50098
                     /locus_tag="ACHA02_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8668951.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="ACHA02_000578"
     CDS             complement(50244..50951)
                     /locus_tag="ACHA02_000578"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8668952.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16843 a         8960 c        10654 g        14942 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acagtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgaaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttctgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac
    51241 tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaattt tatttagtaa
    51301 tcattgcttt aaaatactac gcttatataa cagagaaaat aagcgttata ataaagaaca
    51361 gtattattta aaatgacaga aaacattact attaaagaa
//
LOCUS       DAYYDS010000009        46782 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000009 DAYYDS010000000
VERSION     DAYYDS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.009"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(186..770)
                     /locus_tag="ACHA02_000579"
     CDS             complement(186..770)
                     /locus_tag="ACHA02_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8668953.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1101..1334)
                     /locus_tag="ACHA02_000580"
     CDS             complement(1101..1334)
                     /locus_tag="ACHA02_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8668954.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1349..2299)
                     /locus_tag="ACHA02_000581"
     CDS             complement(1349..2299)
                     /locus_tag="ACHA02_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8668955.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2296..3138)
                     /locus_tag="ACHA02_000582"
     CDS             complement(2296..3138)
                     /locus_tag="ACHA02_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8668956.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(3140..3859)
                     /locus_tag="ACHA02_000583"
     CDS             complement(3140..3859)
                     /locus_tag="ACHA02_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8668957.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            4407..4709
                     /locus_tag="ACHA02_000584"
     CDS             4407..4709
                     /locus_tag="ACHA02_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8668958.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            4781..6007
                     /locus_tag="ACHA02_000585"
     CDS             4781..6007
                     /locus_tag="ACHA02_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8668959.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(6095..6487)
                     /gene="rpsI"
                     /locus_tag="ACHA02_000586"
     CDS             complement(6095..6487)
                     /gene="rpsI"
                     /locus_tag="ACHA02_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8668960.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(6503..6931)
                     /gene="rplM"
                     /locus_tag="ACHA02_000587"
     CDS             complement(6503..6931)
                     /gene="rplM"
                     /locus_tag="ACHA02_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8668961.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(7465..7932)
                     /locus_tag="ACHA02_000588"
     CDS             complement(7465..7932)
                     /locus_tag="ACHA02_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8668962.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(8052..9590)
                     /locus_tag="ACHA02_000589"
     CDS             complement(8052..9590)
                     /locus_tag="ACHA02_000589"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8668963.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9670..10356
                     /locus_tag="ACHA02_000590"
     CDS             9670..10356
                     /locus_tag="ACHA02_000590"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8668964.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(10557..11084)
                     /gene="msrA"
                     /locus_tag="ACHA02_000591"
     CDS             complement(10557..11084)
                     /gene="msrA"
                     /locus_tag="ACHA02_000591"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8668965.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(11168..11845)
                     /gene="pgmB"
                     /locus_tag="ACHA02_000592"
     CDS             complement(11168..11845)
                     /gene="pgmB"
                     /locus_tag="ACHA02_000592"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8668966.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11842..12972)
                     /locus_tag="ACHA02_000593"
     CDS             complement(11842..12972)
                     /locus_tag="ACHA02_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8668967.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(12972..15155)
                     /locus_tag="ACHA02_000594"
     CDS             complement(12972..15155)
                     /locus_tag="ACHA02_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8668968.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(15152..16177)
                     /locus_tag="ACHA02_000595"
     CDS             complement(15152..16177)
                     /locus_tag="ACHA02_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8668969.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(16222..17049)
                     /locus_tag="ACHA02_000596"
     CDS             complement(16222..17049)
                     /locus_tag="ACHA02_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8668970.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(17046..18113)
                     /locus_tag="ACHA02_000597"
     CDS             complement(17046..18113)
                     /locus_tag="ACHA02_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8668971.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(18139..18975)
                     /locus_tag="ACHA02_000598"
     CDS             complement(18139..18975)
                     /locus_tag="ACHA02_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8668972.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18972..19841)
                     /locus_tag="ACHA02_000599"
     CDS             complement(18972..19841)
                     /locus_tag="ACHA02_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8668973.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19911..21194)
                     /locus_tag="ACHA02_000600"
     CDS             complement(19911..21194)
                     /locus_tag="ACHA02_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8668974.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(21208..22893)
                     /locus_tag="ACHA02_000601"
     CDS             complement(21208..22893)
                     /locus_tag="ACHA02_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8668975.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            23168..24193
                     /locus_tag="ACHA02_000602"
     CDS             23168..24193
                     /locus_tag="ACHA02_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8668976.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24788..28441)
                     /gene="rpoC"
                     /locus_tag="ACHA02_000603"
     CDS             complement(24788..28441)
                     /gene="rpoC"
                     /locus_tag="ACHA02_000603"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8668977.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(28520..32137)
                     /gene="rpoB"
                     /locus_tag="ACHA02_000604"
     CDS             complement(28520..32137)
                     /gene="rpoB"
                     /locus_tag="ACHA02_000604"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8668978.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32865..33833
                     /locus_tag="ACHA02_000605"
     CDS             32865..33833
                     /locus_tag="ACHA02_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8668979.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33851..34756
                     /locus_tag="ACHA02_000606"
     CDS             33851..34756
                     /locus_tag="ACHA02_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8668980.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34753..35559
                     /locus_tag="ACHA02_000607"
     CDS             34753..35559
                     /locus_tag="ACHA02_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8668981.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            35716..36684
                     /locus_tag="ACHA02_000608"
     CDS             35716..36684
                     /locus_tag="ACHA02_000608"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8668982.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(37001..37525)
                     /locus_tag="ACHA02_000609"
     CDS             complement(37001..37525)
                     /locus_tag="ACHA02_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8668983.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            37812..39623
                     /gene="scm"
                     /locus_tag="ACHA02_000610"
     CDS             37812..39623
                     /gene="scm"
                     /locus_tag="ACHA02_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8668984.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(39778..40764)
                     /locus_tag="ACHA02_000611"
     CDS             complement(39778..40764)
                     /locus_tag="ACHA02_000611"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8668985.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(40787..41353)
                     /locus_tag="ACHA02_000612"
     CDS             complement(40787..41353)
                     /locus_tag="ACHA02_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8668986.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(41367..41783)
                     /locus_tag="ACHA02_000613"
     CDS             complement(41367..41783)
                     /locus_tag="ACHA02_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8668987.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(42240..44174)
                     /locus_tag="ACHA02_000614"
     CDS             complement(42240..44174)
                     /locus_tag="ACHA02_000614"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8668988.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            44476..45411
                     /gene="pplA"
                     /locus_tag="ACHA02_000615"
     CDS             44476..45411
                     /gene="pplA"
                     /locus_tag="ACHA02_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8668989.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            45501..46604
                     /locus_tag="ACHA02_000616"
     CDS             45501..46604
                     /locus_tag="ACHA02_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8668990.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            46617..>46782
                     /locus_tag="ACHA02_000617"
     CDS             46617..>46782
                     /locus_tag="ACHA02_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8668991.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLAT"
BASE COUNT        13843 a         9747 c         8517 g        14675 t
ORIGIN      
        1 atgaccggaa aaaaaatatt taaagtaatc aaggttaacg gattgatttt tgacactctt
       61 tcacctccaa aaaataagat cgcactgtta tcttaagctg ataaaaatgc accgttattc
      121 agataacagt gcgatcttat caaaaagcat aactgagaac aattctcaat gcaagctttt
      181 tttaattaac tcagtaattc aatccaccca ggaacagaaa attgatacaa tcttctggtt
      241 aatagcatag tcttttcttt tgaatagcta tgcttcaata tatcatagaa tgtagcgtag
      301 aatgagtaaa tgtagaagtg gatttcttct tcattaaaga ctaaattttg attgatcatt
      361 ccgatagtct ttaacttatc tagacaacag actgtctttt tcgtcacaaa tgatgtaatt
      421 ttttcaacaa tatcttgata tttggttcca gctgattgga ataaaatcaa atcgaatata
      481 cgtttattct caaaaatata attaataaat ggatctaatt cattgaagtt ctctctccaa
      541 cttttctcta tatctatatt ttctaagttt aagaggtatt tggtaaactc ttcattatac
      601 atattaataa gcgcattaat atgcggctga ataatttctt caaataacgc ttccttagaa
      661 gtaaaatgtt tataaaaagc acccgtagtt acgcctgcgg tcaggcacag ttgccttatc
      721 ttagtttgct gaaatccttc tttttcaaaa tggcttagtc cactttctat taacttttgg
      781 tgagttataa tataatctct ggtttctatg ggtacttcct cctaataaga tgaattaact
      841 ttattatctt acacaggact tgaagtttca atcgttaacg tttattactg aactcattcc
      901 ttagagctaa actaatgaga aagagcagaa taactgatac aaaaagcaat cctacaagaa
      961 ttaatagact catctaaatc attcaccttt tctaaaataa tccgagaaag atttaaaaaa
     1021 attatatcca gctattagga taagtcctat cataatcact ccaatagcca ttttcttatc
     1081 ctccctaaaa ataaagtctt ttacttaaaa ccttttttgt aatcgtcaat aaattttttc
     1141 cctttatcag ttaattgaaa tcctatatct aaatagtcct catatctaag tttagccagc
     1201 atctcactta cagatgctgg actaacactt agaacttttg ctaaatcatt attttttaat
     1261 ttcttttttc tttttgataa ttcctgaatt gctagtaaat aaattagtat agtagtcgtt
     1321 ttaatttgta ccatacctac cctccaaatt atttcgaaag gccttcagca attttatcca
     1381 ggttccattt catcatcgca tagtaactat caccatcttg tcctttttct gcaattgaat
     1441 cagtaaaaat cgttgaatag ataggaatat tggtatcttt tgatactgtt ttcattggtc
     1501 tatcatccac actgctttct acgaataagg agggaacttt tgttgtgcgt aatttttcaa
     1561 ctaagtgttt tatttgatct ggtgttcctt cttcttcagt gttgatttcc caaatgtaag
     1621 cagaaggcac attatacgct ttcgagaaat atttaaagca tccttcactt gtaacaatca
     1681 tttttttatc attcggaatt gaagcaaatt tagatttagc ttctttgtct agagaatcca
     1741 atttttcaat atacttatct agattttctt tatagaattt tttattatca ggatcttttt
     1801 ccgctaactg tttttcaata tttttagcgt aaataatacc gttttctaaa tttaaccaag
     1861 catgaggatc ttccttccct ttctcactct gaccctctaa gtaaataaca tctatgccat
     1921 cacttgctgc aaaatagtct ttgttttcct ctttgttcgc atttttaact aattttgtaa
     1981 accaagcatt tcctccagtt tccaagttaa caccgttata aaaaatcaaa tctgcttttg
     2041 aagttttttg aacatcttca ggcaaaggtt catattcgtg gggatctttc ccaataggta
     2101 cgatactgtg taaatcaatt ttatcttttg ctatattttc ggtaatatct gctaaaatcg
     2161 aattagtaac tactactttt aatttttcgt tcgaagccac tttatctttg tctttactat
     2221 ttattgaaca tgccccgaat ataaaaagag caatcaaaat ccccagactt cccagtactg
     2281 ctttccattt tctcatcata acgatctctc cttctttctt aaaaattttt gttttggtga
     2341 gattagaaaa ctaattacaa ataagaatgc cgctgttaaa acaatacttg atcctgctgc
     2401 tatgttaaaa ctataaccaa taaacaatcc aagcaatgat gaacttgctc cgactgttgc
     2461 agaaataaat atcatagttt tcaagctatt tgcatataaa tatgctgtag ctgctggtgt
     2521 aattaacatt gcaacaatta atatagttcc cacactttgc atagctgtta ctgctactaa
     2581 agttaataga accatcaata gataatgata aaaactgaca ttcatcccta ttgcttttgc
     2641 cattaatgga tcaaaagaag tgattaataa ttctttaaaa aatagaacta ctataaccaa
     2701 cactaaaacc gaaacgccaa ttgtaatcca cttatcaata tcttgtacag ctaaaatatt
     2761 tccaaataaa atatgaaata aatccgttga gctatttgca actcctataa ggataactcc
     2821 tagtgctaaa aaagaactga aagtaatacc aattgctgta tctcctttta ttacactatt
     2881 gctttttata aaagtaataa tgatagaagc taatagacca aatacaatcg caccaataaa
     2941 aaaattaata cctaaaatat aagaaattgc tactcctggc aaaacagcat gagaaatagc
     3001 atcacccata agagacattc ctcttaaaat aataaaacaa cctactgttc cagaaacaat
     3061 ccctataact acagatgtaa ttagagcatt ctgtaaaaag tgaaagtcat acagaccatt
     3121 aataaaattt tgaagcatat tattccccct ctccaataaa tatagtatct ccataggctt
     3181 tttttaaatt ttcttcatta aatacagatt ctacacttcc gtgagcaatt agatttcgat
     3241 tcaaaagaat aatattatca aaatacttct ttaccttgct taaatcatga tgcacaatta
     3301 gcactgtttt tccctgatgt ttcaattttt ttaacgtatc cataattatt cgttcactaa
     3361 ctaagtctat ccctacaaat ggttcatcta aaaatatgaa atccgcattt tgagcaagac
     3421 atcttgctaa aagtacacgt tgaaattgtc ctccagataa ttctcctatt tgatgattag
     3481 aatattttcc catatcgacc ttttccaaag cctttgaaac tctttgccat tctgcatttt
     3541 taattctttg aaaaactttc attccggcat atgttcccat agatacacat tcttttactt
     3601 ttattggaaa agtaaaatca atatcagctt tttgctctac atatgcaatc ttcaccagtt
     3661 ttttattgat atcttcatta ttaatggaga cctctccctg atgagggata atatttaaca
     3721 ttgctttaag caatgtggat tttccagcac catttggacc aataatacct gttaaagcac
     3781 cttcttgtat ttttaaagaa atctcgttca atgctaaaaa atcgttataa caaacattaa
     3841 gctttttcac ttctaacata actttctcct tctaaatgat aatgattatc acgatcatat
     3901 ggtatacaaa aattatattt tagtcaaccc taaattttat agtttagatt tttttctgtt
     3961 gctgaagatt agaatgacaa aactatttga aactacgcgt atagtaagta gctttcaaaa
     4021 tatataaaaa aggagcgttc tcaaatgaga acatgctcct aaaactactt tattggcgta
     4081 tggaaaaatt atacttgaag tggatagcgg gttgaataag ttgataagca tcaaaacatt
     4141 tataaaaatt attcaagact atgtctgttc catatacaaa tatgaaaatc agtaaactcc
     4201 atttaacttt tttcgtagtg tgtcttttta tacatgttta tcgttattaa tagtgaatag
     4261 gaaaaaaact atttttgatc attgacaatt aaatacaatt tttgaataac gatccctgct
     4321 attcgagcta gaaactcaag tacgccatga cctgattagc gacaaatact tagagatttc
     4381 tttttgttct gggaaaacaa aatgccatga caatagcagc aaataatgat acttccgtaa
     4441 ggctcgccgt aaactgggga gaggtgaaat tcctatgtgc ttacccaaag caattcaaaa
     4501 atttcataac tactatttta ggagatgatt ttatagaatc cagagtctac ctgaataaaa
     4561 aacaaacctg tgcatttcta ggaatcgata ataatactct agataagtgg attcgtatat
     4621 ataatttccc agtaataaga gttggtggtg tagttcgctt tgacgtagaa gatattaaag
     4681 actttatgaa tacatttaaa ataacttgaa attttcttct cgccgcatat actaaaatta
     4741 gtgaaatata ctgccttact ttttttaagg agtgaggatt ttgcctacaa agctttcaaa
     4801 tgggaaatat aaaactaatc tgagatatcc aaaaaggttt aaagaaataa caggaattgc
     4861 atcagaaaag tatcaaaaaa catttccaaa taggcaattg gcgattaaag ccgaaaatga
     4921 tatgaagaag aaaatagaaa aagttttgag agaggagaat gcaaactcat tagaattaaa
     4981 aggaaaaata acgtttaaaa aattttacga atctaaatgg cttcctagat atgaattagg
     5041 tcaaacaatt agatcaaaca ggcctcctag tgatataacc attagtaata caaaagatat
     5101 atttcggttg catattttac ctatgtttgg tgaatacgca atgaattatc taaatatcaa
     5161 tactgagata atatcagatg aattaaccaa aaaatcgaaa gaatatgcaa atataaaaat
     5221 cataaaagga tatgttcgtt ccatgttcga tattgcagaa atactaaatt atatcgaatt
     5281 taatcgaact actaaaatta ttcaaagtat cactgcacct aaaaaaaatg ctttagaaga
     5341 aaaaagaata caagaaggta agcaagcact atcatcaaaa gaacttacta attggataga
     5401 agcagtaaat gatgatttta ataatcattt acttactttt catgattaca cactttttat
     5461 gattaccctg tatcttggtg atagaaaaag tgagacttac gctttgcaat ggaaatatat
     5521 tgattttgaa aaacaaacgg tccgtcttaa acatacttta gataaatatc aaagaaaaaa
     5581 gtttacaaaa ggtcgaaaag ataccgtcat tcaagtacca gaagttgtaa tgacattgct
     5641 aagtgaatgg aaatcggtac aagctgatca attgttgaaa ttaaaaatta agcaaacgtt
     5701 agatcagtat ctctttactt atactaaacc atctggagaa gtaaattgtc ctgttcatgc
     5761 cgattaccta aactatcgaa tcaattcaat taaaagaagg catcctgact tagttcattt
     5821 atctccacac aaactccgtc atacttatgc aactatagct agacaaggtg gagcagatat
     5881 gaatcaaatt tcaaatgctt taacacactc agatatttct acaacaaaga tatatgtgaa
     5941 cacaccagat atagttgata aggctgtttt tgaagctttt cagagaggat tgaataagtg
     6001 tgattgatat gtgttcttaa atgtgttctt aaaaacaaaa gaagcacttt ccaaataatt
     6061 gaaaagtgcc agaaacgttg atatataaag gatattaacg ttttgagaat tgtgaagctt
     6121 tacgtgcttt cttaagacct ggttttttac gttcgaccat acgtgcgtca cgagtaagta
     6181 agccagcacg tttaagagct aaacggaagt caggatcaac ttctagcaat gcacgagcga
     6241 taccatgacg gatagctcct gattgtccag cgtatcctcc gccgtttacg ttaacaaata
     6301 cgtcatatgc gccttttgtt tcagtaacag cgaaaggttg attgattact tcacgcaagt
     6361 cagcatgtgg aatgtattct tcaacatctt ttttatttac agtaattttg ccagttcctg
     6421 gtactagacg tacgcgagca actgcgtttt tacgacggcc tgtgccgata tattgaactt
     6481 gtgccaatga aattccctcc tattagatta agtttgtgat gtctaaaact tctggttgtt
     6541 gtgctgcatg tggatgttct gctccaccgt atacgttcaa ttttgtaaat tgtttgcggc
     6601 ctaaagtgtt tttaggaagc atacctttta cagaagtttc gatcaaacga cgagaatttt
     6661 tagcacgcaa ttcgccagca gtgattgatt tcaatcctcc tgggtagttg ctgtgacggt
     6721 aataaatttt gtcagtcgct tttttacctg ttaatttcac tttgtctgca ttgatgacga
     6781 tgacgaaatc gccagtatcc aaatgaggtg tgaaagttgg tttattttta ccacgtagta
     6841 cagatgcaac aactgttgaa agacgtccca atggaacatc agttgcgtct actacatacc
     6901 atttacgttc tacttcgcct ggtttggcca tatatgttgt acgcacgtta ttttcctcca
     6961 atttaagttc tgatgtttga catacacttc gagtttccgg ggctctttgt ggggcaaaca
     7021 ataccattta acatattacc cgctaatcta gtcaatgtca acgtttttct gcttgattga
     7081 aaaatttata tgaaacttta cttcttttct ttcttttata taaaagggtc gtttcctgtc
     7141 aaaatgaaaa acaaaaagag gctgtgacaa aagtcttgtc acaacccctt attttgaata
     7201 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatccataaa acataagaag
     7261 ctgtttttta aaattgtttc ttgtgactcg gagctgaacg gcaggcttac aacctcttgg
     7321 taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa aatatgagga
     7381 actattttga cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt
     7441 ttcggtcaag ttcaacaaca gtttttacgc gtcgatctct ggcgctttgc ccaaatgagc
     7501 ttggatcatc cagatacgtt tttctgtggc tgttttgaat tcgataaaga tatcttgtgt
     7561 tggatcgtcg ccttcttctc cagaaacttc gattcctttt tggtaaagat ctgctagata
     7621 acgatagcct gcaacaagtg tttccaaacg ttctggaata gtacgatccc agcggccaat
     7681 ttcatcaggg atttttgtgt tatctgcaaa ttcacgcaat gtagaataag gtgaaccgtc
     7741 taatgtgatc aagcgttcgg atacttcatc taactgagcg ttcaaatcat ccatgaactc
     7801 atccatcatt gggtgcaatg taaggaaacc tggtcctctc atgtaccaat gtgtttggtg
     7861 aacaacaaca gacatttggc ttagatctgc aactaattga tttaatactt ctttggtttg
     7921 ttggaatttc atctttcttg cctcctaaca ttttcttaca cttaaaataa tagtagactt
     7981 tcaatgaaaa gtacaacaaa atcatttacc tgagaacgat tttcagttcg cttttttatc
     8041 cgtaaagcca atcatttttc agaatcagct aattcttctc gcagttgagc aaattgctga
     8101 taagtctgtg ctaaatgctt ttgtgtcgaa acaaataatg gatagatttt ctgatatttt
     8161 ttatgatcag tttcttgtac tccaacttgc tctttcaatt ctatcaattc ctgtgcttca
     8221 ctgatacttt ggatctttcc gatgcttctc cagcctaaaa tcacagctcc taatgcggat
     8281 gcttctgtga cagtagggaa aacaagcggg taacccagta catcagccat catctgacga
     8341 aaaatcggag attgagcaaa cccgcctgta gcacggatct ctgtgagctg attttcctgg
     8401 caaactcctt ttaaaatccg ataaaggttg agacagatcc cttctagaat cgccttcatc
     8461 atttcttttt ctgtatgttg ccgctgtaaa ccgataaaac tgccaaacgc atctgcgtcc
     8521 caaagcggtg ctcgctcacc aagtaaatag ggatgaaaaa tcagaccatt ggcacccgct
     8581 ggcacaaaag cgatagccga aagcagtgca tcatatggat tttctccttt ttctttagca
     8641 gattgaacga cttcctgtaa aaatgtatgc cctgcccaat caaagatgcc agcaccattg
     8701 ctagtcgctc ctcccactac ccaatgtttc tgatccagaa cataacaaaa cgtctccatt
     8761 tctggatgga actgaggctg agtagtaata aaacgaagtg ctccgctcgt tcctacggtc
     8821 aaagcggcga tcgtatcacc aaaagcacct aaccctagat tagaaagcgg cccgtctgcc
     8881 cctcccaaaa caaatgatat atcatttggc aaacctagtt ctgctgctgt ttttgatggt
     8941 agacctgtta cttgataggt ggaaggaaag acttgcggca attgctctat tcgaatgttc
     9001 aagtagtcaa gcacttcttc tgcccattgg aattcatgga tatcaaaata tccagttcct
     9061 gaagctgttc cgtagtcgct gatcagttcg ccaaacaatc tataaaagat atagtctttg
     9121 ataccgacaa aataagcagt ttttttgaat aattcaggtt ggctgttttg cagccacttg
     9181 attttgttca atggtgtcat tggatgaatc ggcgtcccag tctgccgata aaattctttg
     9241 ccaagcacgg tatatttcag tttttctgct tcgtccgctg ctcggttatc tgcccaagtg
     9301 atcattcgag ataacggtgt tttctcttca tccaacaata tcaggccatg catcgcagag
     9361 gagaaggaga caaattgaat gattgtgtct gaaggcaact gtttaattac ttcttttata
     9421 acggtgcaga caccttgaaa gatcaattcc tgatcttgtt ctgccatccc ttgtgtttct
     9481 tggatcaaag ggtagcgaaa ataagcggat gctgcttctt gggcttcatc tgtgaaagca
     9541 actgctttgg tttgtgtggt tccgatatct aatccgattg aaacgttcat tttttcacct
     9601 caaattttat gtatgtacaa aaatttagtt taagcttgcg tatcttggtt ttgaaaaggg
     9661 ggagatggcg tgttacttta ttttatcatt ggttgctgta tcggatcatt tttatgttta
     9721 accgcccagc ggattccgtt aggtcactct attatttatc ctagatctca ttgtgtcaaa
     9781 tgctgtcgtt cgctttcttg gtatgaattg attccgattc tctcgatcat cgtccaacga
     9841 tttcgctgcc gctattgcag gtgtcggttg cccttctact atttgttggc agaagcctta
     9901 tgtggtggtc tttttgcctg gttctttact ttttcttccg caagaaattt ttcgacgttc
     9961 atatggatgc tttcggcttt gctattttcg ttgatggatt tattttactt catggtccat
    10021 tctcacacgc tattctgttc ttgggcaatt ttatggattt tttggttgca ggccggcgtc
    10081 ttccaatggc aaagtgtgct gttaacgttt atcgtaggcg gtgtgtgcct tcgctatggt
    10141 caatcctatc tcggagctgg cgatactctg ttgatgcttt cttggagtgg tggcttgtct
    10201 ctagaagagt tgcttcagat cttgtttctt gctagtctgt ttggaatatc cgttttttct
    10261 gtttacttta tctgtcataa aaaaaagttg gaaaagctgc ctttcattcc ttttttgagc
    10321 agtgcgttgc tgattgtcct tcatcttagg tattgaaaaa aggttgtgac aaacgacttg
    10381 tcacaacctc tgatatatag tgttctctta ccaaatcctt gtgacttgga gctaggcgac
    10441 aggttcacaa cctctccact aaatggtgtt caatcagctg accctcagta ttagctcaaa
    10501 atgctggttt tacaacctct gatatacggt gttccataag tagctttttc ggcttcttat
    10561 ttcttgacgc cccaatgtcg acgaaggaaa cctgcacgtc cagaaccttc tcgataaaga
    10621 ttgtaatgga gcttgttttt cttgtaataa tcctgatggt aatcttctgc aggataaaat
    10681 tcgcttcttg gttcgatttt tgtcacgatt ggcttatcga atcgcccgct gtcttgcaat
    10741 gcttgttttg aacgctctgc aatatttcgt tcttcttcat cattatagta gatcactgga
    10801 cgataagaat caccgcgatc agcgaactgt cctaatgcgt cagtcggatc ggtttgctgc
    10861 caatagattt ccaccaactg atcataagaa ataacttctg gatcatattc gatctctact
    10921 gcttctgtat gccctgttgt gcctgttgtt acttgttcat aagttggatt tggtacatgc
    10981 ccgccagtat aaccggatgt cacagaaatg atccctggct gctcttcaaa tggtttgacc
    11041 atacaccaaa agcatccgcc tgcgaaaact gctttttttg tcatataaaa tccccctttt
    11101 ttctcatcat accttgattt ccaaaaggag gataacgttt tgcttgtgac agaaaaaacg
    11161 gaaactatta tatacgtgat acggcttctg ataaatgcca aaacgaacgt acttgatcaa
    11221 aaacggttaa atcaggaatc acatattcag cagaaggaag atttatgttt tcacctattc
    11281 ccaatacatg catgttacca gccaaagctg cttgacaacc agcttctgaa tcttcaatga
    11341 ccaagcatgc atttggcggt acattcaatt gctgtgctcc tttgagaaaa acttctggat
    11401 caggttttgc ttttgatact tgcgtgccgt caataaggac atcaaaataa cttgtcaaat
    11461 tcgttttttc taaaatgagg cgtgcatttt tgcttgctga tcccaatccg atcttgatgt
    11521 ttgttttttt caagtactca agaacagcta ttgcaccagg caaaacggat gtttcatcca
    11581 ttttttgaat cgcttgaaga tatagattgt ttttttgctc agcaaaggct gatttctctt
    11641 tttctgagaa tttctgttcc atttttccaa atgacagcaa tcgctctagt gactcggttc
    11701 tggatattcc tttcagttgc tcgttttctt tctcagtgaa tggaatggac agctggttag
    11761 ctaaaaccag ccacgcttcg taatgataac gagctgtgtc tacaagaacg ccatctaaat
    11821 cgaatagtgc tgctttgatt ttcatcgatt acctccttga aaatgaacga cttgaagtaa
    11881 agatgcgatg tttttagctg tgcgatgaag attatcagtg gcttgctgta aagcttcttg
    11941 taaaggcaca gctgcaggaa ggatcggaaa aacggccgaa atgccaacat ctgctgcttt
    12001 ttcgcttcct ttaccaacac ttccgcaaat agctatcaca ggaacgccct ctggtactct
    12061 tctagcaatc ccaactggcg cttttccttt tagactttgt ccatctagac gtccttcgcc
    12121 tacgattacg aggtctgctt gctgtacccg tttatcgaac tccagtaaat ccaatacgta
    12181 gtcgatgcca gagactattt tccctgaagc aaatgctaac agtcctgcac ccattccacc
    12241 accagcacta ctgcccgatg cctcgattac ttgaggggca aatctcatat aaaaatcatg
    12301 catggcttgg tcagccactg ctaattcatt ttctttcaag cctttttgtg gtccgaaaac
    12361 aaaagaagcg ccttcttttc cgcataaaag attagtaaca tcagtaatga cagtgatttt
    12421 ggctttaatc agctcaatag gcacgttcgt tttatcaact gtagcaattt tacctagatt
    12481 ttctcccagt gcttctactt tttgattatc ttgatcgtaa aaagcataac ctagaccttc
    12541 tgccatgccg atgccgccat cgttggtact acttccgcca acgccgatga tgatgtgtcg
    12601 aacaccttgt tgcaacaatt gtcgtatcag ttctcctact cctcgcgtgg ataacctaag
    12661 tggctgttgt tggtttagtg ggacatcttt caatccgacg atttctgcca tttcaaaaat
    12721 cgcagtttga ccgtctgaag cgtactggat ttctttgttt ttgccaaatg cattagtaac
    12781 gactgctgtt tgccgcttca accttaaact gtctgctaat gatgaaaccg ttccttctcc
    12841 tccatcgcct ataggtagca gatcatattc agcctgaaca aaaacttctt gaaaacctga
    12901 ttgaatggct tgtgctgcct ctaaggctgt cagactttct ttgaaagaat ctggcgcaat
    12961 cagaatacgc attattttcc cctccgttcc aagatcagtt ctttttccag ctggtattgg
    13021 tctccattga caaaaatagg taatgtttct ttcgtctctt tttttattac tatttgtttt
    13081 tttgtcaaaa caaatagcaa cctgtgattt ttccaaataa aaggaaaaga aattttttcc
    13141 catgctgaag gaagtcttgg agaaatagaa agagttccat tcgttactgt aatacctgaa
    13201 aaaccaaata taactgctag ccatagtgct cctaatgaag cagcatgaat tccgtcatct
    13261 gatgaatgcg gatctggtcc taaatcaatc aggcacgctt cttggaataa atgatacgcc
    13321 cattccattt cttggatacg gcaagcctct atggcgtgaa tcgctttact caacgatgag
    13381 tcgtggattg ttttgttttc atagtaatgc aaattcttag aaacgattgg ttgagaaaaa
    13441 tactcaggta ataagtataa cagcatgaca agatctgctt gcttgctgac ttggagttca
    13501 tttacttcgc tacgggcata atcaagtaaa atagactggc tgccaggacg ttctttgtaa
    13561 gtggtcaaat caataactgg cttactgaga tacgtgtcat cttgcggaat cagctgttct
    13621 ttgttaggca ctggcaaata taacttatct aaaaaatttt gacattcttt caaaaaaagc
    13681 ggatcgccag cttgatattt aacagctaag gccacattat aatgcgccag atagttcgta
    13741 tatgcattat tgtcgatata ttctgtgtat tcatctggac caatgactct gtgtatctct
    13801 aaatgatcat cgacttttac agctctgctc agccaaaaag ctgctgtttc atgaatgagc
    13861 gtttttccct ttgtttctaa aaacgattga tcctctgttg cttggacata ctcttgaacg
    13921 gcataagcaa ggtctgcact gatatgatgt tctgcggcag ctgaagcaac ttcttgtctt
    13981 gttccggttt tgatattcaa tgcagcaaat ttgggtgtct cttcctgacc agtgtgtgcg
    14041 ctttcccatg ggaaaagtgc accttgatag tgataatctc ttgctttttg ccgtgcgcct
    14101 tctaatcgat cataacggta cagcagcaaa ttctttgcta cttcaggaaa ctgatataaa
    14161 aagaaaggca ggataaaaat ttccgtatcc cagaaaacat gccctttata accttcgcca
    14221 gttagtccct tcgcccctac actaacactt ggatcattgc ggggagtcat aatctgtaaa
    14281 tggtaagcag cgaagtccat cgctaactga tcgaaagaag aatcgccttg gatcgttatc
    14341 cgatgctgat gccaaaaatc tgtccaataa gacatcgact gctctaaaat ttctgtataa
    14401 ctaacgcttt tctgggcttg ggcaatactt ttcttaaacg ggtctgtttg ctgaagagag
    14461 gtagagagca cgatgatctt ctccagcgta aaggacgtat tttttctgat cttttctgaa
    14521 aaggaagcgg tgatccggcg attttttgct tggaatacac ctgctttatt gaatttagct
    14581 gcaatcccaa tatcaatttt actttctgtt gtctggtagg atgcactgat caaatcgtca
    14641 ccaaatactc gcagttcttt ttcaaccaac tgctgtgtac caaaattggt ttgctgtcca
    14701 tcgatacccg ttttcacatc tacttggcag tcttcggtca acggcttgat gaccactttc
    14761 agggctgcct gatggagatt attttgatca gcaacacgac aagtaatgac ttccactttt
    14821 tttccttgag cagtttcaat gagcgcatag cgtttaagct ctcccatttc taaatctagt
    14881 gcccgctcgt atgcaagaac tttcatcgtc tcaagagaaa aatgttcacc gtcgattttt
    14941 atttcccatc tgatgacatc tgggagattc actaactcgg ctgattctgc tcctaaaggt
    15001 cggttatata caccagctgc aaacattccc cttacttgat tccgatagtc ttcctcctgg
    15061 cttcctctga taccaaaaaa tccatttcca atggtcatct gactcgcata cttattgata
    15121 tactctaaat caaactgttt ttcttctact ttcataaggt aataacttct cctgtttctg
    15181 ccgaacgata taaattttcc accagattct ggatgacata tccttgccag ccatctgcaa
    15241 tcatctgagt ctggtcaccg agcgcccgat tcacaaaatg agccatactt agttcatgtg
    15301 ggtcattaac tggcgtggtc ttttgtaagg aaaccagctt tccatctaca tcttcgaata
    15361 gttcgcctgg atacagatta gcacctgcct gatcgccaca aaaagccaca ttcagtttgt
    15421 cttctctgat attcaaagca aacgaagtat tcagttggat gatcttgccg ttttctaatt
    15481 ccagtgtgcc aaaaattgca tcttctactg tgaatttgct tggatcccat ttcccgaatt
    15541 tccctgaatt ttttttcgta ccaatttttt ggaagctgta cgcccgaacg ctttttactt
    15601 taggaaatcc agtaatatac atcgctgtat caagcatgtg aatgccataa tcgatcaaag
    15661 gcccgcctcc ttgaagttct ttgtcgataa acgttcccca gcctggaaca ccagaccgac
    15721 gaagcgcatt cgcttctacg taataaatat ccccgagaac atcaagatga ttcttgatca
    15781 ttgttgcttc gtcagaaaaa cgatgctgga atccataagc caaaatctgc ccgtttttat
    15841 cggctgtctc tgccatcttt cttgcttgat cagctgtcat agctggtggt ttctcgcaca
    15901 tcactgaagc attagcttcg agggcagcca atacatgttc gaagtgaaag cgattgggtg
    15961 tacagacact cacgatatcg ggagaaatct cattcagcat ttgtgtaact gatgtatacg
    16021 catgagaaaa tcctgtacgt cggcaaaagt cttctgctcg ctggctgact ggatcaacca
    16081 cagcggccaa ttctaagaca tctgatcttt tttgataata agcagcatgt actttttctg
    16141 ctacttgtcc agcgcctatg attgctactt tcttcattta tttctctctc ctttgcttat
    16201 ttacttattg ttattctttc tctattcttt ctctaaatca tttaaatgac gcgctgtttc
    16261 cagacagttt ttcaagtatt gcaatgattc ctgataaatt ttcacttcat tttctcctcg
    16321 caaacgacat tcatatacca tataaccttc atactcattt tgaagtagcg tttgaaaatg
    16381 gcgagtaaaa tcaattgcgc cagatcctgg ttggtagcga tgattatctg ctaaatggat
    16441 atgacccaca tactcttttt gcgtagtcaa tgcttttgaa atatcatctt cttcaatatt
    16501 catatgataa aaatcagcga tgatctgcac atgtttcaat tgattagagt caatgtaatg
    16561 acgcgcatcc gctaacaaat tgatcatatg gtcttggtat cggttcaaag gctccaaata
    16621 aataaccgta cctgtttctt cggctacttt atctaaataa cgaagtgatt cgctgactgc
    16681 tttatagtct ccttctttgc ttcggggtga aaccatcggc ggtaatcgat aagtgaacat
    16741 tccccaagct gctgggataa cgattccttt gcctccaacc tcgcttagtg cacgcaaaat
    16801 ctcagcaatt tgacgtacac cattaagacg ccgttcttcg ataaagtcac cgatccaccc
    16861 atcgtagccg ttacaagctg tcactactgg gatccctgtt tctttgattg cctgtttgac
    16921 tgtgtcgata ttttctatta acaacttacc gtcgatttcg tattcttcaa atccacagtc
    16981 tttgataaat ctaaattttt ctaaaatgtc tcttgggaaa aatgcttgat cttgcgtacc
    17041 gaatttcatt gttctcctcc tcggacttga acgcccattt tgatactttc ttctgggtgc
    17101 tggtctacat aacgcatgta agcttctgcg ctttctgcaa atggcacaac tggatcaatg
    17161 atttcttcac aatccaagta accgttcatc aataattccc aacatgtgtc ctcgattcgt
    17221 ttacggttcc atcttgggta atctgggttt ggttcactag atgctcgagc aaagacgatt
    17281 tttgcattat tgaaatgtgc ttcgcgacca agattaaacc cagctggaaa aggtttagca
    17341 aaggcaacat aagaaatgat cccgccatag ccgatcccta ttaaagaagc ttgtaatgcg
    17401 gcagcactgc cactcgtttc aataactgaa tctactccac gtttatctgt caattccttt
    17461 agacgaaaac cgacatctgc tttcatagga tctagcgtgt aatcggcacc gtgtttttct
    17521 gctaattctc tacgctgcgc tatgggatcg atcccaacga tggtagtagc acctgctttt
    17581 ttagctaatt gaatcgcaat ttgcccgata gcgcctaaac cgaagattgc tacagcatca
    17641 cctgctcgaa catgtgcttc ccggatacca cctaaagcga attgagcagg atcgtaacat
    17701 actgcatttt tccattgatc caaccgatca agtttacgta gtttataatt atcaacaccg
    17761 tttgtaatca gcgtttcacg aatgggtcca taagtgcaga ctaactctcc tttttgatac
    17821 tctgtcacca aactgcccgt ttcttcgatc gtaccgacaa tcatatttcc taattgaaat
    17881 tcgccaaata ccacaccgcg ttggcttcct tcttctcttg gcaagaatag gcgccattct
    17941 tctgaaaatt cttcatcgat aaatggactt aatcctctaa agtcgacaat ttctgttcct
    18001 ggttttggag tagcgaaaat tactcggata cgtacttcgt tcttttcgat ctcacggtct
    18061 tcgtaatctt tgaatccggc tattcttggt tctaatgcaa ctaatttttt catcttttct
    18121 ttcctctttc ttttcttttt atccttttac gccgccttcg actaaatcac ctttaataaa
    18181 tcgttcagat aacgcataca taatgatgac tggtaaagcg gtcaccaatg aagcggccat
    18241 cattcgtccc caaatgtagt ctggtgtact gaataatgtg ttcaaaccga ttggtaaagt
    18301 aaatttttcg gaggtcgaca ggaaaatcga agcaaataaa taatcattcc acgaaaccat
    18361 aaagcaataa acgaagacag aaatgattcc agaaatagcc aatggtataa tgatccgaat
    18421 aatgatctgg atccggctaa gaccatcgat cattgctgct tcttcaatat cagtcggaat
    18481 cgtgtcaaaa taacttttta gcatgaatat cgcagtagga agtgtttgaa cgaccatcgt
    18541 gataatcagt gctgtattgg tatcatacaa acctagaccg gaaatgattt taaataatgg
    18601 aacaacgatc aaaataccgg aaaacatata aacggtgtaa aagctagcat tgattttcga
    18661 gcgagctttg aagcgtagtt ttgacaaagc gtatgccccg ctgatcccaa tcactactgc
    18721 agttacggag gctatcaagg agatcactaa actatttttg aaatacatga tgaatggaaa
    18781 aatctcagca ttgaaaatat cctgataatg ttcaaaagtc cattctttcg gaaaaatagt
    18841 ttgtgtcgtt gcaattgctt cttgtgaatc cttgaatgat gtcatcagca tgatcaaaaa
    18901 tggaaacaaa ttgacagcta atagcaacaa aatcgtcgcg taaaataaga ttttcctgct
    18961 ctttttattt ttcatttcaa gtccaccttc tttcgtgaga gcaagacgac cgctgcaata
    19021 atgatgaaca atagaatcga tatagctgct gcttttccaa gatcattgaa cgcaaaggct
    19081 gtctgataca aataaacgcc taagatattg actttattcg tcagtagata gacatctgta
    19141 aacatataga acatccagat agatcgtaaa acaatcacag ttgctagtac tggtttgatt
    19201 gccggcagtg tcacgatctt gaacttctgc caagcatttg caccgtccat ttcagctgct
    19261 tcataaagcg ttttgtcgac tgtttgcaaa atagccaaaa atgaaataaa cgcatacgga
    19321 tagtatctcc agatagcaaa caaaacgacc ataaagaagc tagcaactgg ctggtcgaac
    19381 cacagcggtg cttcgttgaa aagctgcaac ctatctacta acagatcatt gacgattcca
    19441 taactattat tgaacatata tttccaagca aaaatcaaag aaatagatgg cgtaacataa
    19501 cttaataaaa tcagtgaacg caccgttttc ctaaatctga acttgcggtt aagcaaaata
    19561 gctacggcta atcctaaagc tgtactgcct gctacaacta agaatgtgta agcaactgtt
    19621 aaacctaaag aacgataaaa ttcactatca cttaaaattt caagataatt gcttaatccg
    19681 ataaaaacgg cgtctgagtt aggattcagc ggcatatcga aaaaactgat ctgaatattg
    19741 gaaagcattg gaatcagcac taaaacagct aataaaagca tgcttggtgc taaaagtccc
    19801 atggctaatt ttaagtcaga atgtttcctt ggacgattca ttgaaacctc cccctgataa
    19861 tctgccaata gctatctact tttttcgaac accattggca gaacttcttg ttatttcgta
    19921 tttttttgtg cgtcagctaa agcttgatcg acatcttcgt ttccgacagt tacttggttc
    19981 actgcttttc cgatcacacc tgagcttgtg atatctccca ttttcgtgaa attcttttcc
    20041 ccgactagtc caaagacttg tacttcatca aaagcactag caatctcaga tggcagctcg
    20101 ccaaaagact tgatgacttc attttctttg taaccatcca gttcgactac ttgtttattc
    20161 actggttgag caccaccagg agacatcaag acccactttt ccatgttttt cggttgagat
    20221 aagaaagctg tgaatgcttc agcagcctct ttttgtttct cgtctagacc agaagaaatc
    20281 gtcaatccag atactgtgcc atagacagct tgatcttttt gtgttgggat agcaaaaccg
    20341 atattttctg agttccccgc ttcataaaca gaaggcaaaa tgtaagttga atagattgcc
    20401 attggtgctg ttccattcat gaaagcatct ttgatttctg tcacatcatt agagcctggc
    20461 atcgtgtatt gggataagtt ttgataaaac gacaatgctt ctctcatctt ttccgtatcg
    20521 atcgttacct cgccttcatc atccaaaaca tttgctccat ttgacaaagc aaattgagag
    20581 aacgcttgct cactcattgt tccttcagca gtaggcatag caattccata ttgtttattc
    20641 tctttatctg tgaaatgttg agcaattttt aaaatatcat cccaattttc tggttctgaa
    20701 aaaccagctt ctgataattt ttctttgtca taccaaatac cttgaaccca gccgctaatt
    20761 ggtgcagcaa tatagctttt tccatcttct gaacgaacaa ggtttttggc tccgtcataa
    20821 taattatctt ctccagcttc ctcgataacg ttttggactg ccgtctgatc aatcagttca
    20881 tctttatcca tcactttagc aaaatcctga ctgacttcca ttacttctgg taatttaccg
    20941 gaacgagcta acgtaacgat ttttgtattg aacgctgatt cttctaccgg cacttgcttg
    21001 attttgatgt ctggattttc tttttcaaaa tcagcaacca gttcattgat cacacttaat
    21061 cgttcctgtt cgacagaaga gtgcatgaat tgaatcgttg tctgttgtga ttcgttagag
    21121 ctttgacttt caccacaagc agtaagaaga aatccagtag ctagcagaca ggtagtaaca
    21181 ataatttttt tcattttttt ccacctctta ttttattatt ttcagccaca tatattcata
    21241 cggctggaat atcaactttc gttcttgact gcaatggata atttgtccag aaaacaggtc
    21301 taggtaatct ccttttggta aaagataacc tgctgattga ttggaaaaat tatggataac
    21361 taagactttc tcttccttcg cttcgcgaat aaaactgata aaagaatctg ctgagtccag
    21421 taagtaaatc aaactattcg gatggaaggc tttttctttt tttcgaatag atagcatctg
    21481 ggtcaattca ccatatactg ctgttcttaa tggatctgtc tgcaactctt tctcaatatc
    21541 tgctaacaaa aatttttgcc gattgatttt tcgattctct cctgtttttt ctacgccttt
    21601 gtaatcattt cgacttccca gcacactttg aatataaatt gccggcacgc ctggtaacgt
    21661 taccaaaaat gaatgagcag ctaaaaagcg tttgcttctc cattccgtcg tatctgttgg
    21721 agaacttaac gcatctagat acgtagcgtt tatctcatat ggactggtcg ttccatctgg
    21781 gtttttcttg tagtttactc gggcaccctc tttcttcagc tgttccacta attcaaggat
    21841 cgttttttcc ggcagaatac ctcgtaccgg attcaatccg ataccgtcat gggaggctaa
    21901 aaaattaaaa aaagttgttt ttttcgatgc gaaatcgatg gtttttaacc attctttcaa
    21961 cttacttgta ttacccgaac gaatcgcttg aagggtcaaa ggtggtagcg aaaactgata
    22021 gaccatctgt gcttcattct cgccattccc aaaataactg atattatctt tgtgtggcac
    22081 atttgtctca gtgatcaata ccgttcctgg agctgcttca tcagcgattg cacggaataa
    22141 ttggatgatt ttatgcgttt ctggcaggtg tatacaagaa gttcctagtt ctttccataa
    22201 aaatcccaca gcgtccaagc gaataaatga agcgcctttt tctaagtaaa acaaaagaat
    22261 atcgatcatt ttcaataaaa cttctggatt tttaaaattc aagtctactt gatcttcgct
    22321 aaaagtcgtc catacgtgtt tttctttccc gtttttgtca atgaacgttg taagtaacgg
    22381 gctggtcctt ggtctagtca cgctgcttgt atctgtatcc agtggcaaat caataaaaaa
    22441 atctttgtac gtatcattca agtttaaata ttcctgaaac caagtacttt cggcagacat
    22501 atgattgcag acaaagtcga acattaaccg tgtttcgctt ctcatttctt ctatatcttc
    22561 ccatgtgccc aaacggtcgt tgattgcctg ataatttaca acggagaaac catcatcaga
    22621 tgtatatggg aaaaatggca agaaatgtac aactctaaat gtatctgcca aatacttttg
    22681 ataaaatttc ttaaatgtac gcaaagttgg ttgctgctct tcaaaaaatt gatccccata
    22741 agtaatcaag aaaatatctt cttcgtccca atgcagtttg cctgcctggc ctttcttctg
    22801 ctgataaaca ctgacttttt gttcaatcat agaggcggtt ttctcagaca aatctgagtc
    22861 gcgataaatc gcttgtaaaa gttttgtata catcggattc ccctttcttt tttgggagcg
    22921 ttcccaacga tttgatcata ttatggaaaa cgcttactgt caatagcatt tttttaaata
    22981 attttgatat acacaaaaat acaaataaga aagcttatgt atcaataatt aatagcttct
    23041 taaaaaaata tggtctctca tgatcatgca agctattcct aaattttcaa aatcatagac
    23101 aaaaaagaat gtctattgta ctatggaatt attctaaaac gctcccaaaa aggaggtgca
    23161 tataaaattg aaacctacta tttatgacat tgcgaaaaaa gctaatgtct caaaatcaac
    23221 tgtctccaga gtattaaata accagccaaa tatctctgat aaagcaaaag caagtgtact
    23281 tcaagcaatc aaagaattga attatgaacc gagcaaaatt gcaagaggac tgtcaggaaa
    23341 tggttttgac gcgattctag cattatctca tcgtacttct catacgacct atggcaatcc
    23401 ttttttttca gatgttattc aatccatttc tgccgtttct gaacaaaata acttcgactt
    23461 gattctacaa acggcaaaaa caccagaaga agagctagaa aaatgcgcga aaaagatcca
    23521 agaaaaaatg atccgaggaa ttattatttt aagctctacg atggatgaaa cacttctttt
    23581 tgaattggat caatataata ttcctattgt ggtaatcgga aaagtcggaa acgactacac
    23641 gaacatctac tcagtggaca cggataattt ccaggatagt tacgatttgg tggaaacact
    23701 gatcaagaat ggacaccgtc atatcggctg tctccatgct ccgacagaag tttatgtatc
    23761 caatgatcgt ctcaatggtt accgtcaatg cttatttgat caccacctag atatccgtaa
    23821 tgaatggatt atagacggcg gctttcgctt agaagatagc atggcagcaa tgaatgattt
    23881 aatgaagcta cgagaaagac caactgcgat tttttctacc gatgcattaa aatcattgag
    23941 tatttacaaa gtggcagaac aatctggaaa gcagatacct gatgatcttt cgatcgcagg
    24001 attcagtgat cccttattgt cctccctatt ttcgccacag ttgactcgga tagacattcc
    24061 gacaaatcag ttaggaatca aagccacaga acttttattc caactaatcc accaccaata
    24121 ccctgaagaa aaaagaatca ttctttcaac cgagattact ctcacagatt cgatccaaca
    24181 acaagaaatg tagaacagaa ttataaaaga acagaaacac tgaggataag tcagttactc
    24241 tgttcttttt tcaatcactc atccctcgtt accccctccc ggtattttag cgaatttttc
    24301 ttattttcca tctgttttag atagctgtgg ataactataa tgtttgtgtt aaactatttt
    24361 ttccacaggg aagaaaaaac tatttttaac cacgttgtgt ataagttttt atagttcgtt
    24421 tttttaaaca catttaccct tatttcaatc catgttattt ggggatactt atctctaaaa
    24481 cataaataga ataacagaca tgtctactgg ttttgtttta ttcgaggaat tattcaataa
    24541 tagctagcaa aatattaaaa ataaccgctc tatcttccca tcaatttatt ttgcattcaa
    24601 aaaatgatct atctttactt ttgttgattg acttctcttg gaagatgatc gttccgatac
    24661 cttaattgga tacgaaccga tgttgttaag atcctttccc acttttcttc tagttagaca
    24721 aattatttta gacaaaccaa aaaagaggtt gtgacaataa tttgtcacaa cctctttttt
    24781 aaaatatcta ttttgtttga ttgttcattg cgtcttctgc agccatttgc gcttcaatat
    24841 ctgaaatact gtagacattt tcgcttgcta caccgacttc ttttggttcc atgtcacgat
    24901 aacgttgcat accagtacca gctgggatga tcttacctat aataacattt tctttcaaac
    24961 caagaagagg atctttctta ccgcggatcg cagcatccgt caagacacga gttgtttctt
    25021 ggaatgatgc agcagataag aaactatttg tttctaatga tgcttttgtg atacctagta
    25081 agacaggtcg agaagttgca ggaacgcctc ctgagactag cgtattgtag ttttgatctt
    25141 taaagtctgc gatgtctaat aatgtacctg gtaagatatc tgtatcacct ggatccatta
    25201 cacgaacttt acgcagcatt tgacgaacca ttacttcgat atgcttgtcg ccgatttcta
    25261 ccccttgcat acggtaaaca cgttgtactt cacgaagcaa gtagttttca acagataata
    25321 catcgcgaac ttgcaacaat tgttttggat cgattgatcc ttctgtcaat ggtgcaccac
    25381 ggtggatcat atcgccttct gctactttca tacgtgctgt ataaggaaca gtatacgtac
    25441 gtgtatctgt ttttcctttg attgttactt ctttggtacg tgttgcagga tcttctgaga
    25501 tatcgatgac atcccctgtt acttctgtga tcactgcttg ccctttcgga ttacgtgctt
    25561 caaagatttc ttggacacga ggaagacctt gagtgatatc gtctccggca accccacctg
    25621 tatggaatgt acgcatcgtc aactgagtac caggttcacc gattgattga gcggcgatcg
    25681 taccaactgc ttctccgact tcaacgtcag agccagttgc aaggttgcgt ccgtaacaat
    25741 gtttgcagac accatgtttt gtattacatg tgaagacaga acggattgtc actgtttcga
    25801 cacctgcatc aacgatttgt tttgcgatgt cttcggaaat caattggttt cctgcaatga
    25861 tcaattcatt tgtttcagga tgacgtactt ctttacgggt gtaacgtccc actaaacgat
    25921 cttctaatga ttcgatgatt tcatttccct cgcggatggc ttggatttca agacctcggt
    25981 cagtaccgca atcttcttca cggatgatta catcttgggc cacgtcaacc agacgacgag
    26041 tcaagtaacc tgaatcggca gtcttcaagg ctgtatcggt catcccttta cgagccccgt
    26101 gagttgagat aaacatttcc aagacggata gtccttcacg gaagttcgaa atgattggca
    26161 attccatgat tcgaccatta ggagcggcca tcaaaccacg cattccggca agctgtgtaa
    26221 agttggaaat gttaccacga gctccagaat cagacatcat gaagattgga tttttcgctt
    26281 ccaaaccttc catcagtttt tgttggatgc tgtcttttgc agcattccaa acagcaatga
    26341 cacgttcata acgttcgtca tctgtgatca gaccacgacg gaattgtttt gtaatagttt
    26401 caacttgttt atgggcattt tcaatgattt cttgtttttc attcagtaca ctgatgtcag
    26461 cgatccctac tgtgataccg gcatgtgtcg aatgtttgta ccctaagtct ttcatacggt
    26521 caagcatctt agaagtttct gttactttga atcgtttgaa gacttctgcg atgatgtttc
    26581 ctaagttttt cttcttgaac ggtaatacta attcttgctc tttgatatgt gcaggaatat
    26641 cagtaccagc ttcaacaaag tatttgtccg gtgtttggac ggtcaagttg aagtctgttg
    26701 gttcattcag ataagggaat tcaggaggca tgatctcgtt gaagatgatc ttaccaactg
    26761 ttgtgatcat cgtacgttct ttttgccatt cagtaaatgg tttttcacct aatgagttag
    26821 ggttcacacc gatacgtgaa tgtaaatgaa catatccgtt gcgccatgcg ataacggctt
    26881 cgttttgatc tctgaagacc attccttcgc cttcgcgtcc ttcttcttcc attgtcaagt
    26941 agtagttacc tagaaccata tcttgagaag gagtaacaac tggtttacca tctttagggt
    27001 tcaagatgtt ttgagcagct aacatcaaca tacgtgcttc tgcttgtgct tcttcattca
    27061 atggaacgtg gacagccatt tggtctccat cgaaatcggc attataagct tcacatacaa
    27121 gtggatgcaa acggattgcg cgtccttgta ctaaaactgg ttcgaatgct tggataccaa
    27181 gtcggtgaag cgtaggtgcc cggttcagca atactgggtg ttccttgatt acgtcttcta
    27241 aaacatccca tacttcgtct tcttgacgtt cgattttacg tttcgcattt ttgatgtttg
    27301 aagctagttc gcgttgaaca agctcacgca tcacgaatgg tttgaataat tcaatggcca
    27361 tttcttttgg aagtccacat tggtacatct tcaagaaagg tccaacaacg ataacggaac
    27421 gacctgagta gtcgacacgt ttacctagca agttttgacg gaaacgacct tgtttccctt
    27481 tcaacatatg agaaagagat ttcaatgggc ggttacctgg tccagtaact ggacgtccgc
    27541 gacgaccgtt atcgatcaat gcatctactg cttcttgcag catacgtttt tcattttgaa
    27601 cgatgattcc aggtgcattc aagtctaaca aacgtttcaa acggttatta cggttgatta
    27661 cacgacggta aagatcattc aagtcacttg ttgcaaaacg tccaccttct aattgaacca
    27721 ttgggcgtaa atctggtgga ataacaggga taacgtccat aaccatccag cttggttgat
    27781 ttcctgaagc gcggaatgct tctaaaatat ccaaacggcg gattgcacgt gtacgttttt
    27841 gtccttgtgc agatttcaat tcttctttca attcggctgc ttctgcatct agatcaacat
    27901 tatcaagtaa ttgtttaatc gcttctgcac ccatagcagc ttggaattct tgtccatatt
    27961 gttcacgttt ttcgcgatat tcgcgttctg tcaacaattg ttttttctct aaattcgtat
    28021 ttcctggttc gatgactaca tatgaagcaa aataaatgat ttcttccaaa gcgcgtgggc
    28081 tcatgtctaa tacaagaccc atacgtgaag gaatcccttt aaagtaccag atatgtgaaa
    28141 caggagctgc taattcaata tgtcccatgc gttcacggcg aactttcgaa cgagtaactt
    28201 ccactccgca tcgatcacaa acgattcctt tataacggat acgtttgtat tttccgcagg
    28261 cacattccca gtcttttgta ggaccaaaaa tccgctcaca gaacaaccct tcacgttcag
    28321 gttttaaggt acgatagtta atcgtctcag gtttttttac ttcaccgtaa gaccagcttc
    28381 tgattttttc gggagaagcc aaacctattt gcatactttc gaatttattt acatcgatca
    28441 aaaggggttc cctccatttc atttaatggc aaaccgcaag acggctgatc ttcgaccgcc
    28501 ttgcagctca tagaccggat tagtctaaat tatcttctgc ggtttcaaag ttttggatga
    28561 catcgtcttt ttcatcttcc acttgttcag cagctttctt ttctaattct ttggcagttt
    28621 gctgttcggc aaattttgtc aaagcatcaa cagtgatcaa atcgtcgtct tcatcgtcca
    28681 tgtcgcgaag ttcgatctct gtttcttcga tatccaagac acgcatgtcc agacctagtg
    28741 attgcaattc tttcaccaat acgcggaatg attccggtac acctggtttt ggaattggtt
    28801 cacccttgac gattgcttcg tatgttttca cacgtccgac tacgtcatct gatttgtatg
    28861 tcaagatttc ttgtaaagta taagccgcac cgtaagcttc cagtgcccat acttccattt
    28921 caccaaaacg ttgtccacca aattgagctt tacctcccaa tggttgttgt gtaaccaatg
    28981 agtaaggtcc gattgaacgt gcatgcaatt tgtcatcgac catgtgagca agtttgatca
    29041 tgtacatcac accgacagac acacgtccat caaatggttc acctgtacgt ccgtcgtaca
    29101 gaacagtttt cgcatcgctg gccataccgg cttcacgaac tgtttcccat acgtcttcat
    29161 cacttgcacc atcaaatact ggcgttgcta cgtggatacc taactgacgt gcagccatac
    29221 ccaagtgtaa ttccaatact tgcccgatgt tcatacgtga aggcacccct aaaggattca
    29281 gcatgatatc gattggtgtt ccatcaggta ggaatggcat atcttcttcc ggcataatac
    29341 gggatacaac ccctttattt ccgtgacgac cggccatttt atccccttca tggattttac
    29401 gtttttgaac gatataaaca cgaactaaca tgtttacgcc tggtgacaat tcatctccag
    29461 cttcacgagt aaagattttc acatcgtgaa cgataccgcc gccgccatgt ggtacacgta
    29521 atgatgtatc gcggacttca cgagcttttt cgccaaagat cgcatgcaat agacgttctt
    29581 ctgcagataa ttctgttacc ccttttggcg tgactttccc tacaagcaaa tcgccatctt
    29641 ggacttctgc accaatacgg atgataccca tttcgtctaa atcttttaat gcgtcttccc
    29701 cgacattagg gatttcgcgt gtgatttctt caggtcctaa ttttgtatcg cgggcttctg
    29761 attcatattc ttcaatatgg atagaagtgt atacgtcatc ttttaccaaa cgacggctca
    29821 tgatgatcgc atcttcatag ttgtaccctt cccaagtcat gaaaccaact aaaacgtttt
    29881 ggcctagagc catttcgcct tgttccatag atggtccgtc tgccagagta tccccttttt
    29941 cgactttctc acccaaatga acgattggac gttggttgta gcttgttccg gagtttgaac
    30001 gacggaattt tgttacatcg tatttatcta atgcaccgtt gtcgcggcgt acgcggattt
    30061 cagaagcatc aacgtattca acgacaccgt catgtttaca taacaaagca gctcctgagt
    30121 catgcgctga tttgtattcc ataccagtac caacccatgg tgcttgagga ttgatcaacg
    30181 gcactgcttg acgctgcatg ttggcaccca tcaaggcacg gttggagtca tcgttttcca
    30241 agaaaggaat acatgctgtc gcaacggcaa ctacttgttt aggtgatacg tccatgtagt
    30301 ctactttatc aatagatact tctaagtttt cactttgagc acgagccata acaacgtctt
    30361 gagcaaatgt tccatcttca ttcaatgggc tgtttgcttg ggctacgatg taatggtctt
    30421 cgatatcagc tgtcaagtag tcgatttggt cagtaacacg acctgtttca cggtctacac
    30481 gacgataagg tgtttcgata aatccgaatt tatttacttt cgcataactg gacaaactat
    30541 tgatcaaccc gatgtttggg ccttcaggag tttcgatcgg acacatacgg ccatagtggg
    30601 agtagtgaac gtcacgaact tcataaccgg cgcggtcacg agtcaatccg ccaggtccta
    30661 aggctgaaag acgacgtttg tgtgtcaatt cgccaagagg gtttgtttga tccatgaact
    30721 gtgacaactg agaagaacca aagaattctt tgatacttgc cacaactgga cggatattga
    30781 tcaattgttg aggtgtcaat gtttcagtat cttggatcga cattctttca cgaaccacac
    30841 gttccatacg tgctagcccg atacggaatt ggttttgtaa aagttcaccg actgaacgga
    30901 tacgacgatt tcctaagtgg tcgatatcat cgacattgcc gataccttcc atcaagttca
    30961 agaaatagct cattgaagca atgatgtcgg ctggacgaac cgtttttaca gctgcttctg
    31021 gatagccgtt tccgatcacg tttacttcac gttcagggtc tcttggtgaa aatactttga
    31081 tgacttggac agtcattgga tctgtgacca caccgtcttc tgatggatag taagtcactg
    31141 aatttaggcc attatcaatg aaaggagcta gtgtttccat gacttggtga gtcagaactg
    31201 ttcctttttc aacgatgatc tcgcctgttt ctggatcaac taatgtttca gctaatgtca
    31261 gattcaatag acgtgttttc aaatctaatt ttttgtttac tttgtaacga ccaacgtttg
    31321 ctaagtcgta acgttttgga tcgaagaaac gagcattcag caagttacgt gagctgtctg
    31381 ctgtttttgg ttcgcctgga cgcaaacgtt cgtaaacgtc tttcaaacct tcttctgttc
    31441 ttgagtcgct tgcgttttta tgcaagtctt tttcgactgt attgcgtaat gtttcgctgt
    31501 cgccgaagat ttcaaagatc gtatcatcgg aaccaaatcc taacgcacga actaatacgg
    31561 ttaaaggaat cttacgtgta cggtcgatac gtacataaga gatgtctttt gcatcagttt
    31621 ccatttctag ccaagctcca cggttaggga taacagtaga accgaatcct tcttttccgt
    31681 ttttgtcgac ttttccatgg aaatagacac ctggtgaacg gactaactga gaaacgatta
    31741 ctcgctctgc cccgttgatg atgaatgttc cttgttctgt cattaatggg aaatcaccga
    31801 agaatacttc ttgtgctttg atttcgccag tctcgcggtt tgtcaaacgc aatgttacat
    31861 gtaatggtgc agagtagttt gcatcatgcg cacgagcttc tgctactgta tactttggtt
    31921 ctttcaattc ataatctaca aattccaaag atagattgcc attgaagtca tcgattggca
    31981 aaatgtcttc aaacatttct ctaagtccct catctaagaa ccattggtaa gagtctgttt
    32041 gaatttcgat caaatttggt aattccaaca cttcactgat tcttgcgaaa cttctacgtt
    32101 cgcgatgttt tccgtatttt actacgtgtc cagccaagct cttcacccct ctaaaatgtc
    32161 attcaaaaag actacccgat caattgttac aataatattt caaatattat ttttaggtaa
    32221 caacagacat tttttgcggc ttttaggcaa aaaaaaacca aaaatagaac attctcttga
    32281 aaccttactt cgtgagcttc tacttttgtt tccttatatt aaagccgaaa cggacaatat
    32341 ttcgtttctg ccttttcttc atcagatagt ttttgcaact tcatatacta cacttatttt
    32401 ctataaaagt caacacttcc tggcgtttct tctgcttttt cgcaacattt tcttaatgtt
    32461 ttcaatttta tgttttcaat tatctgacaa ttcttaattg acagaccttt ttatctcggt
    32521 tatactttag aaaattcatt ttgtggagga acttctatgc gaaccaacat caaccaaatc
    32581 gcaccaacac aactgaataa cttttacttt agctacttta gataggtttg tattcatcat
    32641 gatggataca aacgttaaac gacagtttgt atctatgatg gctaaagaac tttgtattgc
    32701 agcaaaaagc cacgtagaca aaatgctaag tggctgagca gtgaaaatac aaagtaaaag
    32761 tatcaagcat tctacttttt caatttattt tccgactttt caatccattt ggacattttg
    32821 tctgaatgga ttttttgttt cccaaaatta aggaggaaaa agaaatgaaa aaaaatataa
    32881 tcatcggatt aggcgctgcc ctgctactag ctttaggagg ttgcgcacaa aaagacaatt
    32941 ccgcagaggc atcagatgga caaaaaacaa ttggcgtatt gcaacttgta caacataact
    33001 cgttagatgc tgcctataaa ggattcaaag aaggattagc agaagaaggg tataaagaag
    33061 gcgataatct aacaatcgat tatcaaaatg cccaaaacaa tcaagacaac ctgaaaagta
    33121 tgagtgagaa actcgtcaaa gaagatcctg atttgctact tggaatcgcc actccagcag
    33181 cacaatcttt attaaatgaa acaaccacta tcccgatgac tgtcacagcg gtcacagact
    33241 tggaacaagc ccatttagtc acatccaatg aaaaacctgg cggcaatgtg actggcacga
    33301 cagatatggt gccaatcgaa aaacaaatcg gcttactgtt aaatatcgtg ccagacgcca
    33361 aaacaatcgg catcatgtat aacaacggtg aagcaaactc taagatccaa gctgatttgg
    33421 cagaaaaaga attgaaaaaa gcgggcgtca aagtcaaagt actgacagca aattccacaa
    33481 atgatgtcca acaagtgacg aaaagtctag ctaaagatgt agatggaatc tatattccta
    33541 ctgataacac gttcgcaagt gcggcaagtg ttgtcggtga agtcgcaaaa gaaacaaaaa
    33601 cacctgtcgt tgctggcgca gttgatcaag tcaaagacgg cggtctagca acagtaggaa
    33661 tcgactacga aagcttgggc aaacaaacag ggatcatggc agcaaaaatc ttagacggca
    33721 agaatccaag tgaacttcct gtcgaaacag cgaatgattt ggaactgtat gtcaataaag
    33781 acatggccaa agcattaggc atcaatccag acagcatcaa agaagctgaa taaaacacag
    33841 gaggattctc atgacaaatt tgctctctct tttcttatca tctgcatcgc aaggggtctt
    33901 atgggcccta cttgcgattg gtgtatacgt cactttccgt atcttggaca ttgccgactt
    33961 gactgctgaa ggaagctttc ctttaggtgc agggatcgcg gcagtaagta ttacaaatgg
    34021 ctatcatcca ttgacagctt gtcttctagg ctttttaggg ggtgcggcag cagggcttgt
    34081 ttccggactg cttcacacaa aattgaaaat tcccgccctt cttgcaggga tcatcaccat
    34141 gactggttta tattctgtca catcccgtgt gatgggtgcg ccgaacatct ctttattggg
    34201 tcagccaacg atctttcatt gggtcgagtc attcggcata acaaaagaca acgctgtact
    34261 gatcgtcggt ctgctttttg ctttgatcgt tgtatgttta cttgttttgt tcttacgtac
    34321 agaaacagga cttgctattc gtgcaactgg cgataatctg gcaatgagcg aagcaaacgg
    34381 gatcaacaca gataatatga agatcatcgg ttatatgatt tctaatggat gcattgctct
    34441 ttctggctcc ttacttgcgc aaaacaacgg ctttgctgat ttaaattctg gtattggaac
    34501 gatcgtaatt ggacttgctt ctattatcat cgcagaagtt ttgttacgta accagccact
    34561 atttctccgt ctcttgactg tcgttttagg tgcggtcatt tatcgtttta ttttagcgtt
    34621 agtcttcgaa ctgaatgttg aaccaagtga ttcgaaactt gcttctgctt tcgtgctagt
    34681 gatctgtctt tctttcccgc agatccagca aaaattccat ttacctcaaa aaaagaaagg
    34741 cggtagtgtc aaatgaatga cgtcttaaca ttggaaaatc tccatcatac ctttgaaaaa
    34801 ggaacgatca acgaaaatca tgtcctgaaa ggcattaatc tttccttaga tcccggcgac
    34861 ttcgtgacga tcatcggtgg aaatggggct ggtaaatcta ctttgctgaa cagcatcgcc
    34921 ggtacatttt ctgtcgatca gggtcaaatt ttattgaatg gaaaaaatat cacgaaaaaa
    34981 tctgttgtcg aacgttcgaa atccatcagt cgcgtattcc aagatcctaa actagggaca
    35041 gccgttcgtt taacggtaga agaaaatctt gctcttgcta tgaaacgagg aaaaaaacgc
    35101 ggcttcttcc gtggtgtcaa accacaagac cgaacatttt tcaaagacca gctggttcgg
    35161 ctaaatttgg ggttggaaaa tcgtttatcc tcagaaatcg gtttgctgtc tggaggacaa
    35221 cgtcaagcta ttacgttatt gatggccaca ttacagcgtc cagaactgat cttattagat
    35281 gaacacactg cagcgcttga ccctgctact tccaaaacgg tgatggaatt gacagaaaag
    35341 ctgatcactg agcagaatct cactgctttt atggtcactc atgacatgga agacgcgatc
    35401 cgctatggga gtcgactgat catgctgcat caaggaagga tcgtcgtaga tatccagcaa
    35461 gaggaaaaac agcagctcac agtgccccaa ttattagcgt tgttcaaaca aaacagcggc
    35521 acggcattaa aagacgatca agtactttta tcttcataat cagaaagagg atgggacaaa
    35581 aaatgaacgc ccaccctctt cttttttctc tttccttctt tcgtttttgc tttatgtatc
    35641 aaccgttgtt tcttcgaaaa ctattttgtt ctctccttct tcctgataga atggatggga
    35701 aaggagcttg atgccatgac aacgaaagac aaggtacttc aattattgaa aaagacacca
    35761 gattttcttt ccggagaaaa gttggcacag caactggaag tttcccggac aagtatttgg
    35821 aaagccatca aagaattgga aaaggcaggc tatcgttttg agcacgggcc tacagggtat
    35881 cgttacctgc cctctgacgt actagatgca aaagaaatcc agcaagagct acggtcgttt
    35941 attcctgatt tgaaagtaca aacgatgttg acttctgaat ctacgatgaa agatgccaaa
    36001 ctggcagcag ctcaaggaga ttctgtttcg aagttatttg ttgctgatac acaagaacaa
    36061 gctcgtggac gttttggacg gccttttttc gctgaacctg gacgaggaat ctacatgagt
    36121 ctcttgctcc atccagacaa acggtttgat gaattgcctc aatatacgat catcgcagcc
    36181 agtgcctgtg tgaaagccat tgaatctgta accggtcgtt ctgcttcgat caaatgggtc
    36241 aatgatatct acatggatgg aaaaaaagtc tgtgggattt tatctgaagc agtcagtgat
    36301 atggaaagcg gcaggatcag tcatatcatc attgggatcg ggcttaattt ttctgttcct
    36361 caaaatcatt ttccagctga tctccaagaa aaagccatct ctgtctttcc cgacggacaa
    36421 gctagtgtca caaggaatca gctaatcagc aaaatctggc aagaattgtt cacttatctt
    36481 tctcacccag aaaaagcgtt ggacttctat aaagaaaaat cattcgtatt gggaaaaacc
    36541 gtgtcgttca atcaaaaagg aacgacttat gtcggtcttg caaaagaaat cggtgatcat
    36601 ggcgaactga tcgtccaaac aaatgaacag aagatgagtt tgtcttctgg cgaaatcagt
    36661 ctcacatcta tccgtacgca ataattatca gatttcacaa aaggacacct gatctcatac
    36721 agtgtccttt tttctgaatt ctcttcaaaa gactctcctt attctatcag ctgacagttt
    36781 ctctatcctc tagaaaaagg tattctaaaa atatctataa agcatggtac actttcttta
    36841 aatgaatcaa tgcaaaaaac aagagagtca tcagtttaca cgatcaaaag agatagcagg
    36901 ccgcttcctt tttcaatctt atagatagac catgggtaac gataaaaaca aaaaagaact
    36961 tgcgacttat tttgccgtaa gttctttttt agctagtgcc ttaatctctt aatcccaaca
    37021 aacgtctcgc atagatcacc tgaccagagt gcatagcagc atcatcaata atagaaacga
    37081 gacgcattcc tcgtgtgact ggtggtgtcc agttctcgtc aattacttca tctagctcgg
    37141 aaggttccag ccttcttaag taagaaatag caaaatcagc agctcctcgc aagtatccta
    37201 gcagatcttc tatttgatta gcttggatct gctttgcttg ttccagacta tgcttccaat
    37261 cttgttcgtt atctgctatc gtcatcggaa accggctttc ccagtcttga ctatgccaaa
    37321 ttggagaagt tcctgctaaa tcagcaatct ggaaatctaa ttctcgagcg gtatgccaag
    37381 ccagccaagt cattgatttg atctgcgggg ctctatcttc cactggaaaa cgattggcct
    37441 cctcaataga gacgccttca aacaaacgtt caaaccgatc gtatgcccga tccacactct
    37501 cgcatagcat atctaaataa tccatttatt ttcctccttt ttatttatcc tacttacttt
    37561 ttcctaattc taggatacct acttatatgt tactacacac ccttattttt tacactttaa
    37621 cagtcaccat catcaatagt ttggagattg aaagaaaaag atagtgtcga actatcaatt
    37681 gaacaagaag caacaatatg aacgtttcat taatattctt taaataaaca atgatttttc
    37741 aaacgaacag tttaagacta ggagaaaaag agtataatag gaaaatgttt gaaaggaaag
    37801 gaaggggaaa aatgaaaaaa agggcaagtt tactagtcct agttgctttg ttgttcaata
    37861 tcttttcgta tggtgtggcc gtttcagcgg aaacatacat tcctaaagac cctgggaata
    37921 tttataatga tctaactggt aactatggct tgttaggaat tgctagtcaa ttccacgtat
    37981 ttgctaaaaa taagacaact gtgaatgcac atacagacgg aaatatagca acacatgaac
    38041 ttgatgcaaa aaataatttt ggtacagagc ttcatgaagg tttattgaac caagaaataa
    38101 attatataca aaaaactgat tctttcgcta attcttcagg catacctaca ggtagcacaa
    38161 gaatgaataa atttgtcgtt ggcagtaacg tggatgtagc acttcaagat ggtcatccta
    38221 cagtcaatgg atccaggctt gatcacttaa ctccagatga tttttataaa gatcgtgctg
    38281 ggcatgaata tattgatttc gagcaggaat ttgctaaatt gaatgtagca gcaaatgatc
    38341 ttgcaacgat cactccagct aaaacatata ctgctgcaga ttttccagac atgaacaatc
    38401 ggacaatcga tttaactgat ttatcagata ccgggttttt attggtaaat atcgatgcag
    38461 aagtgttaac gatgaatacg ccacttcaaa tcattaatcc aaatgatcaa gtagttgtat
    38521 ttaatgtcat caacagttct tctgcattga acgtacagtc accaatcaaa tacaatgatc
    38581 gttcaaatca tgaaacagaa gatttttcgg atgcgaatat ctcttggaat tttggaaatg
    38641 agatgactga tttgacaatc agtgctcctt ttcaaggaac aatccttgcg cctaatgcta
    38701 ctatccgcat tactcaaaac caagacggaa cgattatagg aacaaatgtg attttagatg
    38761 cagctaccaa tcgttgggat cctaacgaaa tatttatcag cgacaatggt acagatacga
    38821 cagacacaag cgacagtaca gatacatcta ccacaagtga tagctcagat tcatcaactt
    38881 caagcgacag tacagatact acctctacca caagtgatag cacggataca tcagcttcaa
    38941 gcgacagtac agatactacc tctaccacaa gtgatagcac ggacacatca gcttcaagtg
    39001 acagtacaga tactacctct accacaagtg acagcacgga cacgtcagct tcaagcgaca
    39061 gtacagatac tacctctacc acaagtgata gcacggacac atcagcttca agtgacagta
    39121 cagatactac ctctaccaca agtgatagca cggacacgtc agcttcaagc gacagtacgg
    39181 atacatctac cacaacaagt gatagcacgg acacgtcagc ttcaagtgac agtacagata
    39241 ctacctctac cacaagtgat agctcagatt catctaccac tacaagtgat agcacggaca
    39301 cgtcagcttc aagtgacagt acagatacat cctctaccac aagtgatagc tcagattcat
    39361 caacttcaag cgatagtaca gacacgtcgt ctaccacaag cgatacacgt catgcaagtg
    39421 tctccttctc aaaaacaagt tcggataaca ggacgaactt taacttaact aataaaacaa
    39481 gcaatgacaa tggaaacaaa tcaaaaagag cacttcctaa aactggaagt caatcgaata
    39541 attggataac actcgcaggc gttatattac ttgtaatcgg tttaagaatc acttttatga
    39601 gttataagaa atcgaaaagg taaaaaaata ttcaagataa aaaagagcaa cgtcgctaac
    39661 ggacgttgct ctttattttt gctttttatc ataaatacaa gaactacaat tcaatttgtc
    39721 aaaaaacacg tagatttcct tctgagaaat ttatttacgt atcgaacctt tccctattca
    39781 gtctttgcga tccaaaatca gttgggtaag ggaaaagaga ttctcttttg ttcgaagaga
    39841 ggtggcaggt aatttcttta tttccttcaa agccttttct gtgtaagagg ccgctaactt
    39901 cctagcttct tccacgcctc ctgtatgttc aacgattttg cgtacggcgt cgacatcttc
    39961 ttctgtcatt gccgctttct tctctaaata aggtagtagc tttggtttgt ttttttgcaa
    40021 agagtagatc aacggtaaag aatagacccc ttgacgcatg tcttctagta caggtttgcc
    40081 gatttcctgg ctctcttttg tataatctaa aatatcatcg ataatctgaa aggccatccc
    40141 gatagaaagt ccgatatctc ctgctttttt tgcgaagcgc tcgcttgttc cactttcaaa
    40201 agcgccaaca gaacaactta attggaacag ttcagctgtc ttacctgaaa tattttccaa
    40261 atattctttt acagaaagtt cataattata gcgatcatcc atctgcccta attcgccatt
    40321 caatactttt tccatactgc gagaattcaa ttggagactt ttcaaggaag tggagtaatc
    40381 agacaatagt ttaaaacagc agacaaataa atagtctcct gcatagacag aaacgacatt
    40441 accaaactga ctacggatat taggctgacc acgtcgcaga tccgcttcat caacgatatc
    40501 atcatggatc agtgtggccg tatggagcat ttcgatagaa gctgccaaag cgatggcttt
    40561 gtctcgatct tgctctggtc caaattgtga gaacaatagc tgataggcag gacgcaacag
    40621 ctttccgccg gcatggatcg tttctttgat ggcttgttcg acaggtttgt ttttcaggcg
    40681 gacagtttct tccatccgtt ttagtgtttc tgtcaattct ttacttaaag caggatagtt
    40741 ttcccacatt ggatgtagct tcataaaaaa actccttgtg tgacgattat tttttggtaa
    40801 acattgggct ccatttttgt ttggttccct gctcataatc tttttggaat cgttgaagtg
    40861 tctgcacatg tcgtaaaaga taattcgctg cgatcccgat tgcaatccct gataaaatcc
    40921 ctaaaactga aaggactggc aagtacaaca tgactgtcca tgattgtgca atgaaagaag
    40981 ccatcaaaag ctgtccgaca ttatgcatga aaccgccggc cgcactaatt ccgatgatac
    41041 tgacacgttt tggtcctaat tgcttgacca ataacattcc aaaataacta agcaaagcac
    41101 cactcatact ataaaggaat gtggaaatcg ttccgcctag caaagtagtc aggaatagtc
    41161 gtagccaaag tagtaagaag ctgtcttgtt ttttcatggt aaaaatggca acgatcgtga
    41221 ttagattggc taagcctagt tttgcaccag gcgcaaaaga aaaaggataa gggatcatat
    41281 tttccaataa accaatgatc actccttgtg caacaagtag cgaaatataa atcatttttt
    41341 gtaatttact catggtgtgt gaaggtctaa taaatcagac tgtcttcgtc acctccatct
    41401 gttgaacgga cttcgatcac taatttatgt ggcaaacaga cgatggtttc gccatttttc
    41461 gttgcccagc ctcgacgtac gcagatctgg tcatcgcaat ctgcttcttt tatccgtact
    41521 cgatcgccat ctatctcgat caagttataa tctccgtgtt catctttgta agtatatgtg
    41581 ttgctctctt ctccagccat caaagggaat gttttgattt ctgtgccatc cacacgcagt
    41641 accgcttctt tttctgctgt tgcggtgttt tgctgaagac caaaaatcat gactggtaaa
    41701 aaagaaagaa cagtcaaaag caaaataata aaaacatccc aaggtttgag cagacttttt
    41761 tcagcaaatt ctttaaggct catttttgtt ctcctttttc cagattaact cacccaattt
    41821 tagcataaaa acaagaggtt gtgacacctg tgtcttgctt tgagtaataa gaacgaagaa
    41881 tagaaaacag ctttccatgt tttctaattc tttgggctta ttaccgagaa gcagcaggtg
    41941 gaacaccgtt tataacaaga ggttgtgaca gaagtgggca acttcaagaa ataagaagga
    42001 atttacgaaa attacttttc aagtttttgt gaaatttcgg cttatttttc gaagaagcta
    42061 cttctggaac accgtttata acaagaggtt gtaacagagg ttgtgacacc tgtgtcttgc
    42121 tcatggaggc ttatctcggg catcttattc ctttaatttt acataaaaag gaggctgtga
    42181 cggtttgtct cagccccttt ctattgaaat cttgcattca aataacgttc tttatctatt
    42241 tatttcttgc cgtactggga acttggtcta ccgtaccacc aattacctag tgtacgttgt
    42301 agccatggaa ttacccagcg atccaaccca atcgcacgtc cggaaccgtt catcaatgcg
    42361 aaagcaacaa aaatgaacca aatgtttacc cagtaaaaca tgcctgataa gcagaacgtg
    42421 atcaccaata caatcgttgt cgcactgcat agccatgtga ataatccagc aatcaatgct
    42481 aatgccaagg caatttcaaa gaataccatg aatttttgga agaacattgc tacatcattg
    42541 tttggcatga tcacttgcat gatctttgtg aaccagtctg gtgacttctc aatcacagcc
    42601 attggttctt caccgtatgc ataactcaaa ccaaaatgtg cagcttgagt cgctgcttct
    42661 ccggcacttg cccctgctcc gctggcagct gtggttgcat caccagcagc agaagcacca
    42721 ctggcaacat ctgttacatt gtattcccat gtccaaggga acacactgtt tccagtgaac
    42781 catgatcctt caccaaacca agtacttggt ttgaacactt caccattccc aatgattttt
    42841 ttggaagctt caacgaacca aactaaacca tagaaaatac gaagtggcac actccataag
    42901 acattgccgt aacgagaaga atgtcctcga gtcacgttgc gatcatcttt gatatggaaa
    42961 aactcatgca ttaaatattg gaacatataa taaccagaac gaatatcaaa gaagtatttc
    43021 aaattcacga tatgtttcat tacgatagct aaaaatccgc ttaaatggat cttatcaaat
    43081 aggttagcta ccccccattt ggcaccaata gaaaccataa atccttgata gtttccttta
    43141 aatttatgct tttctccgcc tttgatcgaa gccacgatat tcgctgcggc tgtgtgtcct
    43201 gtttgttcag cagcttgaac gatctgcggt gtcggtgttc ctgggaattc ttcataaaag
    43261 actaaatcac caatgatata gatgttttta tcttcgtagc cttttgcctg catatattca
    43321 ttggcgatca accgctgtcc gcgtgctgat tctaatccaa agtcagcagc gtcagaagtt
    43381 cctttgacac cagctgtcca aatcagtgtg tgagtcggaa tgttgcgtcc gtctttcaat
    43441 ttgatatgat cagcagccac ttctacgatt ggtgcattca ataataattc cacgttgtgc
    43501 ttacgcaaat agcgttcagc ttttgcagca tcatttcgat ccaacatatt caagatcgtc
    43561 ggcattgcct ctacgaccat cagtgtgatt tcgtttggat ctaatttata atctttggct
    43621 aggcgatttt tccagtcgat cagttcccca atcatttcaa taccggtaaa tccagaacca
    43681 cagacaacga aagtcagcat ggctttacgc agttcatcat ctggttccaa tgcagctttt
    43741 gctactgtct tctcaatatg ttcccgaatc cttacagaat cttcaaatga ccaaagggtg
    43801 aatccgtgtg ctttgacacc aggtgtacca aagtcattcg gctctccgcc cattccaagg
    43861 atcagctgat caaaaggata ggaacctaat ttggtcttga cgatttttcg ttctttatcg
    43921 ataccgatta cagtgtctgt gacaagttgt acattctttt gacgagcaaa caaatgctgc
    43981 agatcgtatt ggattgcttc tggttctaca cgtccgccag ctacttcatg caactcggtc
    44041 atcatagtat ggtacgaatg acgatcgatc agagtaattg ctacatcttc ttttttcagt
    44101 ttctttgcca agtattttgt agctgaaaca ccggcatatc ctgctcctac aacgacaata
    44161 ttttgctttg ccattgatca tccgctcctt atagactatc tatataatga atatatgata
    44221 attcttttag tttgatactt attacacaat ctccatatat tatatagatg ttctgttctt
    44281 ttgtctaact ccttttggat ttttttcaaa ttttaaaata aagcgttttt tcacgaaatt
    44341 tgctgaaaga aaacgtttga aatattacat gggttattta tcaaaaatag ttgacttata
    44401 ggctaaaaag attagaatgt ataatgaatc acaaagaaaa attgtggact agaaatggaa
    44461 tggtggggta ttaaaatgaa gaaaaaacgt tttgtaactg gatttgccgt attagcattt
    44521 tcagctctag tgttaggcgc ttgcggcgca gatgacaacg gaagctcaaa ctcttcttct
    44581 gaatcatcta cagcacaatc ttcgactgca aaaagcacaa cagaatcatc tgcaaaagtt
    44641 gtagctggcg gagatttgca agacggcaca tataaattag aagaaaaaaa ttattcaaat
    44701 ggttaccgtg cagtctttga aatggttgta aaagacggca aaatcactga atcaaaatat
    44761 gataacgtca atgaaaacgg cgaatcaaaa acaaaagatg cagaatacaa caaaaacatg
    44821 gaagcaaaat ctggcacaaa cccagaaaaa tacattcctg agttgaatga ccaattctta
    44881 aaagcacaaa gcgcaagcgg tgtggaagtc gtaactggag ctactcactc atctgaaagc
    44941 ttccaaaact acgcacaaca attgatccaa gctgctcaag ctggtaacac agatacaatc
    45001 gaaatcgaca atggtgctga tttgaaagac ggcacttaca agcttgaaga aaagaactac
    45061 tctaacggct accgtgtgca attcgaaatg acagttgctg gcggcaaagt aactgaatca
    45121 aactttgatt atatcgacaa agacggtaaa tcaaaacaag atgatacaga atataatgaa
    45181 aatatgaaag caaaaagcgg tacagaacca aaaacttaca tccctactct gaacgacgaa
    45241 ttcgtcaaag caatgggcga agaagatggc tcacctgcta atgtagaagt tgttacaggt
    45301 gctacacaca gctcacattc attcatcatg tacgcacaac aattagtgaa tgcagctgaa
    45361 aaaggcgaca ctcaaacaat cgaagtagat aatatcgtaa cagaaaaata atcacataat
    45421 catactaata aaagttggag ggtgggacga tagccagatt aggcagtcgt ctcacctttc
    45481 aaaatgaaga gaggaaaaag atgcggaaaa aactactatt ttccttaatc acactgggca
    45541 tctcttttat ggtgctaact ggctgcagca actcagcaaa taaaacaacc actgaaagtt
    45601 cagcagctac caagatattg aaagatccat attctgatga acagttcttg ttagggactt
    45661 acgttcgtat ccgtgtgtat gatgaaggca aagaatcagc attgaaacct gccttcgatc
    45721 gagtaaaaga acttggtgat aaaatcacga tcaatcaaaa aggttctgag atcgatgaag
    45781 tgaatgagca agctgggatc aaaccagtaa aagtttctga tgacgtctac acgttagtca
    45841 agcgggccta cgaatatagc caagattctc aaggcggatt cgatatggcg attggtgcaa
    45901 tcacacaatt atggcgaatc ggttttgatg acgccagaaa accttcccaa gaagaaatcg
    45961 atcaggcatt gaaattagtg gattatcata agatcgaact aaatgacaaa gaaaaaactg
    46021 tttatctaaa agaaaaagga atgatcatcg atttaggtgc aatcgccaaa ggttacatta
    46081 cggacgaagt agtgaaagtc ttgaaaaaac aaggagtgac cactgccatc gttgacttgg
    46141 gcggtaatgt ctatgtctta ggccacagcc caagaggcga aaaccaagac tggacagtcg
    46201 gcatccaaga tccgaacatg gctcgcggat ctgttttagg ttctattaaa gaacggaaca
    46261 aaacattagt tacctctgga atttacgagc gttatttaga agtagatggc aaaaaatacc
    46321 atcatttgtt tgatccaaaa acgggttatc cgttcgacaa tgatatcgca agtgtcacga
    46381 tcataacaga taaatcaatt gatggtgacg ggttgtctac agcagtcttt tcaatgggtg
    46441 tgaaaaaagg actggaatac gtcgaaagcg aattgaataa cgggacggaa gcgatcttcg
    46501 tgacaaaaga cgataaagta tatgtgaccg atccaatcaa agacaccttc aaattatcgg
    46561 aggactccca ttacacgatg ggtgaccgtt ctgaactaaa ataaggagta cgaaccatga
    46621 cactaaaaat ctttttagaa gtagttgaaa tacgaacaaa ggtggctagt gtatttcctt
    46681 ttgcggtagg ggtattattc tctatcgctt tttttcatga gatacattgg ctaaacactt
    46741 ttttattttt tattggaatg ctagtttttg atttagcgac ta
//
LOCUS       DAYYDS010000010        44939 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000010 DAYYDS010000000
VERSION     DAYYDS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.010"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACHA02_000618"
     CDS             complement(38..1189)
                     /locus_tag="ACHA02_000618"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8668992.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACHA02_000619"
     CDS             complement(1202..1639)
                     /locus_tag="ACHA02_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8668993.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACHA02_000620"
     CDS             complement(1654..3420)
                     /locus_tag="ACHA02_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8668994.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACHA02_000621"
     CDS             3684..4361
                     /locus_tag="ACHA02_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8668995.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACHA02_000622"
     CDS             4364..5491
                     /locus_tag="ACHA02_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8668996.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACHA02_000623"
     CDS             complement(5691..6131)
                     /locus_tag="ACHA02_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8668997.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACHA02_000624"
     CDS             complement(6142..8175)
                     /locus_tag="ACHA02_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8668998.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACHA02_000625"
     CDS             complement(8177..9880)
                     /locus_tag="ACHA02_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8668999.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHA02_000626"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHA02_000626"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8669000.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACHA02_000627"
     CDS             complement(10963..11988)
                     /locus_tag="ACHA02_000627"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8669001.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACHA02_000628"
     CDS             complement(12197..13066)
                     /locus_tag="ACHA02_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8669002.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACHA02_000629"
     CDS             complement(13525..15417)
                     /locus_tag="ACHA02_000629"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669003.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACHA02_000630"
     CDS             complement(15414..17141)
                     /locus_tag="ACHA02_000630"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669004.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACHA02_000631"
     CDS             complement(17157..17807)
                     /locus_tag="ACHA02_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8669005.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACHA02_000632"
     CDS             18011..18307
                     /locus_tag="ACHA02_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8669006.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACHA02_000633"
     CDS             18479..19099
                     /locus_tag="ACHA02_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8669007.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACHA02_000634"
     CDS             complement(19191..20357)
                     /locus_tag="ACHA02_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8669008.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACHA02_000635"
     CDS             complement(20369..20722)
                     /locus_tag="ACHA02_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8669009.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACHA02_000636"
     CDS             20850..21872
                     /locus_tag="ACHA02_000636"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8669010.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACHA02_000637"
     CDS             21891..22052
                     /locus_tag="ACHA02_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669011.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACHA02_000638"
     CDS             complement(22311..22745)
                     /locus_tag="ACHA02_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669012.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACHA02_000639"
     CDS             complement(22855..24714)
                     /locus_tag="ACHA02_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8669013.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACHA02_000640"
     CDS             24715..24855
                     /locus_tag="ACHA02_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8669014.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACHA02_000641"
     CDS             complement(24832..25992)
                     /locus_tag="ACHA02_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8669015.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACHA02_000642"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACHA02_000642"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8669016.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACHA02_000643"
     CDS             complement(27739..28437)
                     /locus_tag="ACHA02_000643"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8669017.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACHA02_000644"
     CDS             complement(28394..30055)
                     /locus_tag="ACHA02_000644"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8669018.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACHA02_000645"
     CDS             30273..31673
                     /locus_tag="ACHA02_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8669019.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACHA02_000646"
     CDS             complement(31925..33004)
                     /locus_tag="ACHA02_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8669020.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACHA02_000647"
     CDS             complement(33090..34049)
                     /locus_tag="ACHA02_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8669021.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACHA02_000648"
     CDS             complement(34061..35572)
                     /locus_tag="ACHA02_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8669022.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACHA02_000649"
     CDS             complement(35594..36157)
                     /locus_tag="ACHA02_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8669023.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACHA02_000650"
     CDS             complement(36163..37026)
                     /locus_tag="ACHA02_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8669024.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACHA02_000651"
     CDS             complement(37040..37936)
                     /locus_tag="ACHA02_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8669025.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACHA02_000652"
     CDS             complement(38111..39400)
                     /locus_tag="ACHA02_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8669026.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACHA02_000653"
     CDS             complement(39372..40889)
                     /locus_tag="ACHA02_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8669027.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="ACHA02_000654"
     CDS             41087..41977
                     /locus_tag="ACHA02_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8669028.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACHA02_000655"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACHA02_000655"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8669029.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="ACHA02_000656"
     CDS             complement(42886..44358)
                     /locus_tag="ACHA02_000656"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8669030.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12989 a         9387 c         7783 g        14780 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgttttt
//
LOCUS       DAYYDS010000011        44814 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000011 DAYYDS010000000
VERSION     DAYYDS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.011"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHA02_000657"
     CDS             212..604
                     /locus_tag="ACHA02_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8669031.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHA02_000658"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHA02_000658"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8669032.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHA02_000659"
     CDS             2698..2943
                     /locus_tag="ACHA02_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669033.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHA02_000660"
     CDS             3165..4775
                     /locus_tag="ACHA02_000660"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8669034.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHA02_000661"
     CDS             complement(4752..4964)
                     /locus_tag="ACHA02_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669035.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHA02_000662"
     CDS             5067..5936
                     /locus_tag="ACHA02_000662"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8669036.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHA02_000663"
     CDS             6101..7372
                     /locus_tag="ACHA02_000663"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8669037.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHA02_000664"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHA02_000664"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8669038.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHA02_000665"
     CDS             8763..9026
                     /locus_tag="ACHA02_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8669039.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHA02_000666"
     CDS             9471..9920
                     /locus_tag="ACHA02_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8669040.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHA02_000667"
     CDS             9977..11395
                     /locus_tag="ACHA02_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8669041.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHA02_000668"
     CDS             11395..12255
                     /locus_tag="ACHA02_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8669042.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHA02_000669"
     CDS             12429..13202
                     /locus_tag="ACHA02_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8669043.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHA02_000670"
     CDS             13349..13714
                     /locus_tag="ACHA02_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8669044.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHA02_000671"
     CDS             complement(13862..15070)
                     /locus_tag="ACHA02_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8669045.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHA02_000672"
     CDS             complement(15089..15367)
                     /locus_tag="ACHA02_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669046.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHA02_000673"
     CDS             15560..16216
                     /locus_tag="ACHA02_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8669047.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHA02_000674"
     CDS             complement(16658..17455)
                     /locus_tag="ACHA02_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8669048.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHA02_000675"
     CDS             complement(17452..18636)
                     /locus_tag="ACHA02_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8669049.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHA02_000676"
     CDS             complement(18661..19908)
                     /locus_tag="ACHA02_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8669050.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHA02_000677"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHA02_000677"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8669051.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHA02_000678"
     CDS             21888..22202
                     /locus_tag="ACHA02_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8669052.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHA02_000679"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHA02_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8669053.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHA02_000680"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHA02_000680"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8669054.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHA02_000681"
     CDS             23546..23875
                     /locus_tag="ACHA02_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8669055.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHA02_000682"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHA02_000682"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8669056.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHA02_000683"
     CDS             24863..25687
                     /locus_tag="ACHA02_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8669057.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHA02_000684"
     CDS             25680..26027
                     /locus_tag="ACHA02_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8669058.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHA02_000685"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHA02_000685"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8669059.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHA02_000686"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHA02_000686"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8669060.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHA02_000687"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHA02_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8669061.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHA02_000688"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHA02_000688"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8669062.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHA02_000689"
     CDS             31684..32451
                     /locus_tag="ACHA02_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669063.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHA02_000690"
     CDS             32441..34483
                     /locus_tag="ACHA02_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8669064.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACHA02_000691"
     CDS             34764..34913
                     /locus_tag="ACHA02_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8669065.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACHA02_000692"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACHA02_000692"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8669066.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACHA02_000693"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACHA02_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8669067.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACHA02_000694"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACHA02_000694"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8669068.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACHA02_000695"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACHA02_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8669069.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACHA02_000696"
     CDS             39330..40412
                     /locus_tag="ACHA02_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8669070.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACHA02_000697"
     CDS             40412..41215
                     /locus_tag="ACHA02_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8669071.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACHA02_000698"
     CDS             41285..42025
                     /locus_tag="ACHA02_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8669072.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACHA02_000699"
     CDS             42036..43040
                     /locus_tag="ACHA02_000699"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8669073.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..>44814)
                     /locus_tag="ACHA02_000700"
     CDS             complement(43085..>44814)
                     /locus_tag="ACHA02_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669074.1"
                     /translation="FIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKD
                     ETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLP
                     ANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIG
                     TFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFII
                     LTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASV
                     ISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILY
                     IIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFV
                     QEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRN
                     IQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFV
                     ICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQ
                     LSRNYPNPV"
BASE COUNT        14396 a         7823 c         9336 g        13259 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcat cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaat
//
LOCUS       DAYYDS010000012        43368 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000012 DAYYDS010000000
VERSION     DAYYDS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.012"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..2389)
                     /locus_tag="ACHA02_000701"
     CDS             complement(8..2389)
                     /locus_tag="ACHA02_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8669075.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="ACHA02_000702"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="ACHA02_000702"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8669076.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="ACHA02_000703"
     CDS             3090..3629
                     /locus_tag="ACHA02_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8669077.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="ACHA02_000704"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="ACHA02_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8669078.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="ACHA02_000705"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHA02_000705"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="ACHA02_000706"
     CDS             4736..5902
                     /locus_tag="ACHA02_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8669079.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="ACHA02_000707"
     CDS             5948..7444
                     /locus_tag="ACHA02_000707"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8669080.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="ACHA02_000708"
     CDS             7525..8208
                     /locus_tag="ACHA02_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8669081.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8853..10043
                     /locus_tag="ACHA02_000709"
     CDS             8853..10043
                     /locus_tag="ACHA02_000709"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8669082.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10145..11098
                     /locus_tag="ACHA02_000710"
     CDS             10145..11098
                     /locus_tag="ACHA02_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8669083.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11167..12516
                     /locus_tag="ACHA02_000711"
     CDS             11167..12516
                     /locus_tag="ACHA02_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8669084.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12699..13022)
                     /locus_tag="ACHA02_000712"
     CDS             complement(12699..13022)
                     /locus_tag="ACHA02_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669085.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13151..14314
                     /locus_tag="ACHA02_000713"
     CDS             13151..14314
                     /locus_tag="ACHA02_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8669086.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14384..15733
                     /locus_tag="ACHA02_000714"
     CDS             14384..15733
                     /locus_tag="ACHA02_000714"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8669087.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15778..16209)
                     /locus_tag="ACHA02_000715"
     CDS             complement(15778..16209)
                     /locus_tag="ACHA02_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8669088.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16597..19239
                     /gene="alaS"
                     /locus_tag="ACHA02_000716"
     CDS             16597..19239
                     /gene="alaS"
                     /locus_tag="ACHA02_000716"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8669089.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19439..20035
                     /locus_tag="ACHA02_000717"
     CDS             19439..20035
                     /locus_tag="ACHA02_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8669090.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20157..21305
                     /locus_tag="ACHA02_000718"
     CDS             20157..21305
                     /locus_tag="ACHA02_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8669091.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21343..21756)
                     /locus_tag="ACHA02_000719"
     CDS             complement(21343..21756)
                     /locus_tag="ACHA02_000719"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8669092.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21835..22266
                     /locus_tag="ACHA02_000720"
     CDS             21835..22266
                     /locus_tag="ACHA02_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8669093.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22365..22598)
                     /locus_tag="ACHA02_000721"
     CDS             complement(22365..22598)
                     /locus_tag="ACHA02_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8669094.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22781..24451
                     /locus_tag="ACHA02_000722"
     CDS             22781..24451
                     /locus_tag="ACHA02_000722"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8669095.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24598..24984
                     /locus_tag="ACHA02_000723"
     CDS             24598..24984
                     /locus_tag="ACHA02_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669096.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            24971..25669
                     /locus_tag="ACHA02_000724"
     CDS             24971..25669
                     /locus_tag="ACHA02_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8669097.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25674..26138
                     /gene="lspA"
                     /locus_tag="ACHA02_000725"
     CDS             25674..26138
                     /gene="lspA"
                     /locus_tag="ACHA02_000725"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8669098.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26135..27046
                     /locus_tag="ACHA02_000726"
     CDS             26135..27046
                     /locus_tag="ACHA02_000726"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8669099.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27281..27820
                     /gene="pyrR"
                     /locus_tag="ACHA02_000727"
     CDS             27281..27820
                     /gene="pyrR"
                     /locus_tag="ACHA02_000727"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8669100.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27834..29117
                     /locus_tag="ACHA02_000728"
     CDS             27834..29117
                     /locus_tag="ACHA02_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8669101.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29130..30056
                     /locus_tag="ACHA02_000729"
     CDS             29130..30056
                     /locus_tag="ACHA02_000729"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8669102.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30075..31355
                     /locus_tag="ACHA02_000730"
     CDS             30075..31355
                     /locus_tag="ACHA02_000730"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8669103.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31360..32436
                     /locus_tag="ACHA02_000731"
     CDS             31360..32436
                     /locus_tag="ACHA02_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8669104.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32441..35626
                     /gene="carB"
                     /locus_tag="ACHA02_000732"
     CDS             32441..35626
                     /gene="carB"
                     /locus_tag="ACHA02_000732"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8669105.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35680..36456
                     /locus_tag="ACHA02_000733"
     CDS             35680..36456
                     /locus_tag="ACHA02_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8669106.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36453..37385
                     /locus_tag="ACHA02_000734"
     CDS             36453..37385
                     /locus_tag="ACHA02_000734"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8669107.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37382..38098
                     /gene="pyrF"
                     /locus_tag="ACHA02_000735"
     CDS             37382..38098
                     /gene="pyrF"
                     /locus_tag="ACHA02_000735"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8669108.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38105..38731
                     /gene="pyrE"
                     /locus_tag="ACHA02_000736"
                     /pseudo
     CDS             38105..38731
                     /gene="pyrE"
                     /locus_tag="ACHA02_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            38758..39036
                     /locus_tag="ACHA02_000737"
     CDS             38758..39036
                     /locus_tag="ACHA02_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669109.1"
                     /translation="MRFVSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFI
                     FLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39074..39715
                     /locus_tag="ACHA02_000738"
     CDS             39074..39715
                     /locus_tag="ACHA02_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8669110.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39758..40450
                     /locus_tag="ACHA02_000739"
     CDS             39758..40450
                     /locus_tag="ACHA02_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8669111.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40447..41349
                     /locus_tag="ACHA02_000740"
     CDS             40447..41349
                     /locus_tag="ACHA02_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8669112.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="ACHA02_000741"
     CDS             complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="ACHA02_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8669113.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13874 a         7663 c         9440 g        12391 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaagc
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8281 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8521 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     8881 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     8941 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9001 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9061 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9121 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9181 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9241 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9301 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9361 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9421 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9481 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9541 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9601 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9661 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9721 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
     9781 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
     9841 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
     9901 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
     9961 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10021 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10081 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10141 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10201 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10261 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10321 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10381 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10441 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10501 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10561 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10621 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10681 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10741 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    10801 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    10861 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    10921 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    10981 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11041 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11101 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11161 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11221 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11281 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11341 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11401 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11461 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11521 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11581 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11641 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    11701 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    11761 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    11821 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    11881 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    11941 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12001 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12061 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12121 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12181 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12241 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12301 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12361 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12421 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12481 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12541 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12601 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12661 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    12721 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    12781 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    12841 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    12901 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    12961 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13021 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13081 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13141 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13201 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13261 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13321 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13381 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13441 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13501 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13561 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13621 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13681 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13741 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    13801 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    13861 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    13921 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    13981 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14041 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14101 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14161 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14221 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14281 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt
    14341 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14401 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14461 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14521 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14581 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14641 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14701 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    14761 tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    14821 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    14881 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    14941 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15001 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15061 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15121 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15181 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15241 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15301 gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag atctagactt
    15361 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15421 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15481 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15541 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15601 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15661 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15721 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    15781 agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    15841 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    15901 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    15961 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16021 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16081 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16141 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16201 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16261 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16321 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16381 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16441 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16501 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16561 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16621 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16681 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16741 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    16801 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    16861 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    16921 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    16981 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17041 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17101 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17161 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17221 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17281 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa ctttgagaca
    17341 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17401 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17461 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17521 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17581 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17641 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17701 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    17761 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    17821 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    17881 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    17941 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18001 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18061 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18121 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18181 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18241 ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat caaaaaccat
    18301 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18361 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18421 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18481 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18541 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18601 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18661 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18721 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    18781 caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    18841 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    18901 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    18961 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19021 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19081 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19141 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19201 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19261 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19321 cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19381 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19441 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19501 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19561 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19621 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19681 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19741 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    19801 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    19861 attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc ttttatttaa
    19921 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    19981 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20041 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20101 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20161 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20221 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20281 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20341 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20401 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20461 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20521 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20581 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20641 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20701 aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca ttatatatcc
    20761 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    20821 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    20881 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    20941 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21001 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21061 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21121 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21181 tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta cgtagtcaaa
    21241 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat tatcttttat
    21301 attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag tctttttttc
    21361 ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat ttttgttttc
    21421 gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga ttctttgaag
    21481 gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt
    21541 gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa
    21601 agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa gcgggataga
    21661 tagctgttct tgtttacctt gatagacaat caagattcca cctccactag gaccagggtt
    21721 gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc ctttttgttc
    21781 aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta gagaatgaaa
    21841 aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc attgaccttg
    21901 atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta ttggaaaagc
    21961 aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcggcg ttcttttatt
    22021 ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg agaaatatct
    22081 gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat gttgcaagat
    22141 gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct ttttgatcat
    22201 tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa aaaaagaaaa
    22261 agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag aaaagagtga
    22321 gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc atgcatacta
    22381 gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc tcgattccct
    22441 tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc ctcttcttca
    22501 atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa gccgtatcct
    22561 tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac ctttctaaag
    22621 tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa ttaaaaacct
    22681 gtaattttat gttttttttc tgaataaaaa aaggtataat tggcatatct taacaaagaa
    22741 gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa atgaagaatg atctgcaaat
    22801 ttcacaagaa acagagctag aaccgattgt tgaagtggct agtaaattag atcttaagga
    22861 agacgatttg gaactgtatg ggaaatataa ggcgaaaatc aatttttctg gaatcaatcg
    22921 atcaaaggat aacgctgagg ggcatttgat cttagttact tccatcaatc caacaccagc
    22981 tggcgaagga aagtcaacga tcactgtagg gttaggagac gcgctcaata aaatcaataa
    23041 aaaatctgtt attgcattgc gagaaccctc acttggtcct gtgatgggaa tcaaaggtgg
    23101 tgctgcaggc ggaggatatg cacaggtatt gcctatggaa gatatcaact tgcattttac
    23161 tggtgacatg catgctatca caacagctaa taatgcatta tctgctcttt tagataatca
    23221 tattcatcaa ggaaatgaat tgaacatcga tgcccgccga gtgatttgga aacgggtagt
    23281 cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc ttgggtgggc caatccaagg
    23341 tgttccaaga gaagatgggt ttgatatcac agttgccagc gaaatcatgg ctatcttgtg
    23401 tcttgcggca gaccttgaag atttaaaggc tcgtcttgca aggatcgttg tcggctatac
    23461 atttgaccgt cagcctgtga ctgctggaga cttgaaagca gaaggcgctc tggcattact
    23521 gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt tacggaacac cagcttttgt
    23581 ccacggggga ccgtttgcca atatcgctca tggctgcaat agtattttag ctaccaagac
    23641 agcattgaga ttagcagatt acgttgtcac agaggcagga tttggtgcag atcttggcgg
    23701 agagaaattt ttagatatca aagtacctaa cttgaaaaaa gcgccggatg ctgtggtgat
    23761 tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg aaaaaagatg aacttaaaaa
    23821 tgagaatctt gatgctttga aagtaggttt tgcaaattta aagcgccata tacgtaatat
    23881 ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa ttcgtaacag atacagacaa
    23941 tgaactcaca ctgttggagc acttgtgtga aaatcagggg attcttgcca agagagcgag
    24001 tgtatgggca gatggagcag aaggcggcgt tgatttagca aaagctgtag tacgattgat
    24061 tgatagaaaa gaagctaatt acaagccatt ataccgccta gaagaaacga tacaagaaaa
    24121 gacagaaaca atcgtcaaga aaatttacgg aggaaatggt gttgttttca gcaaaaaagc
    24181 gcaaaaacaa atagaagagt ttacgaagaa tggatgggac aaactgccga tttgtatggc
    24241 aaaaacacag tattcatttt ctgatgatcc ttcactgcta ggtgcaccgg aagatttcac
    24301 catcacgatc cgcgaaatca tcccaaaatt aggggcagga tttttagtag ccttgactgg
    24361 agatgttatg acgatgcctg gtttgcctaa aaaacctgct gcattgaata tggacgtgac
    24421 aaatgacgga gaagtgttgg gattgtttta agcttgatta taatctacca tcgaaggagc
    24481 tgtattttga ctacagcttc tttttttaga cgatagggaa ttaattttgt ttacactagg
    24541 atcattcttt ttctttattg tattttgagc tatactaaag cataatagga gggtatgatg
    24601 aaaacgacat ttataagaaa aaccggtttt tacggtatgg gtagctcgat tcagctaaga
    24661 atcaacaatg aaaaacgcag tgttctcagt catgaacaaa agataacgct tgatctggaa
    24721 gtgcccttta cgatgcaggt caccttttat tggctaaaaa gccccgttta tacagtgaaa
    24781 gaagcaaagc ctgcttatgt gatcacaatg aattacttga ttcttcaaat ctatccgttt
    24841 ttatttctag caactgggtt gggggccgtt ttcctacaaa atttattttt cagttttttt
    24901 gcagttattg tgttgattgc gttctttttc tatataaaaa accgggcata tttgatcaag
    24961 gaggaaaatt atggcaaact ctgatctttt tttaatcagt ttcaatcgaa tcgaaaaatg
    25021 gatgagagac gagatgggaa atgcacgaaa tatgggattt accgaacttg tcagacgcct
    25081 agctcaaaga aaacaattaa tgatcagaaa atacgaagat gatcttttac agcttgctca
    25141 gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat tttgtcattg cagagccaaa
    25201 tgattggaca gtcaagcgaa tccagctgat cgaacaagaa ctgattcagc ctgaaaccgt
    25261 gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa gatcttccgt tacttgaact
    25321 gttaaagata gtagcagaaa aaaggtattc acaatttccg ttatacaata aaggaaaatt
    25381 tgtagcattg attactttac gtaatatcgg gttttggcta gcaaaagaaa gccaaaaagg
    25441 tccggttgat ttaacaaata aacgagcgtt ggatttgatc atccaaaacg gaaagtacac
    25501 aaattatcat tttgttccag ctaaaacaca tatttacgag gttgaagcga tgtttcgtga
    25561 gcaagggact ttggaagccg tacttatcac taaagatggg aatcccgatg gtaatctgct
    25621 cgggatcgta cgacctcgtg atatttataa acaaatagaa aaggattgat tttttgttaa
    25681 ttatttattg gatattcagc gctttgctag taggagtgga tcagttggtc aaatggtgga
    25741 tcactgataa tctagcgctt ggagagacga aaaatcttat accaggtatt ttgtcactga
    25801 atcatattcg aaacactgga gccgcatgga gtatgctaga agggaagatg tggttcttta
    25861 ctatagtgac actcattgca gtcgttgtga ttctcacatt gatgattaaa aatcgtgaaa
    25921 aaggtaatcg ctggttcatg attggattga gtttgattct agctggtgcg attggcaatt
    25981 ttatcgaccg tattcgtctt ggctatgtag tagatatgtt ccagacagat tttatgagct
    26041 ttccgatttt taacgtagca gatgtgacac ttgtatgtgg agtgatttgt atgttaattt
    26101 atattatttt agatgaaaag gatcaaagaa aaaaatgaca gaacttaaaa cgacgattca
    26161 ccatgaatct ggtaggatcg ataaagtatt gactaattta ttcaccgatt acagtcgttc
    26221 gcaaattcag ctgtggctga aaaatggagc ggtttctgtc aatggacagc cagtcaaagc
    26281 aaattacaaa gtaaaacaag gagacgagat tactgtcttg atccccgaac cagaaacatt
    26341 agatgtggtc ccagaaaata tcccgttgac gatcgtttac gaagatgaag atgtagcggt
    26401 gataaacaaa cctcaaggaa tggtcgttca tccttcagca ggtcatacgc atggcacaat
    26461 ggtcaatgcg ctgatgtacc atatcaagga tctttctacg atcaacggcg ttattcgtcc
    26521 aggtattgtt caccgaatcg acaaagatac ttccggactg ctaatggttg ccaaaaatga
    26581 ccaggcacac gaatcattag ctcagcaatt aaaagataag acctctttgc gcaaatatgt
    26641 tgctttagtc catggagaga ttcctcacga aaaaggaacc atcaatgcgc caatcggccg
    26701 ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat gggaagtcgg ctgtcacaca
    26761 ttttactgta ttggaacgtt tcgaaggatt tactctggtt gaattaaaac tagaaaccgg
    26821 aagaacacac cagatccgtg tgcatatgaa gtatatcggt tatccgttag ctggagatcc
    26881 agtatacgga ccgaagaaaa ctttgaaagg caatggtcag tttctccatg cgcaaatgtt
    26941 aggttttacg cacccgacta caaacgaaaa gatgatcttt gaagcacctc ttccagaagt
    27001 ttttgaaaaa actttagaaa aattaagaaa aaacattgca ttttaattta gataacggta
    27061 tagtaattaa caacgaaagc ataaaactta atagaattcc tttaagttta gtcccgtgag
    27121 gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat aagtagattg ctcattgaca
    27181 tagtatagct atcagtgtat cagtatacgc tcagtctaaa cattcctcct gtcctcttag
    27241 ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa atgcaagcaa aagaagtagt
    27301 agatcaggta accatgaagc gagcattgac tcgaattact tatgagatta tcgagagaaa
    27361 ccatagtatc caagatatcg tattggtagg tatcaaaaca cgaggaatct acattgcatc
    27421 aagaattgcc gaacgcctga agcagctaga agatatcgat attcctgtag gagaactgga
    27481 tatcacttta taccgagatg acaaaaaaga aaatccagaa gaaccggaac ttcattcttc
    27541 tgacatccca gtttccttag aaggaaaaga agttattttg attgatgatg ttttgtatac
    27601 aggacgaacg attcgagcag caatggacgc agtaatggat tttggcagac caagaaaaat
    27661 ttcattagca gtcctggtcg atcgtgggca tcgagaattg ccaatccgtg cagattatgt
    27721 tgggaagaac attccaacat ctcgggcgga agagatttta gtagagatgc aagagcttga
    27781 tggacaagat cgcatcatga ttttgaaaga ggaagattag aggagagaga aacatgtcgg
    27841 agaaaaaagg atttcataat gatgacgtgg tgttagatat ccacgatcgc ccaaccccag
    27901 gacattgggt aggattaagt ttacaacact tgttcacgat gttcggagca accgtgttag
    27961 tccccatctt agtaggtatc gatccaggta ttgcgctagt cagttcagga ctaggtacat
    28021 tggtgtactt atttactact aaaggaaaga tacctgcgta cttaggaagc agcttcgctt
    28081 tcattgcagc gatgcagctt ttgatgaaaa cagatggtta tccagcgatt gcacaaggag
    28141 cagtcacaac tggattagtc tatttgattg ttgcgctgat catcaaaaag atcggttcag
    28201 catggctgga taaaatcttg cctccaatcg tcgtcggtcc agtcatcatg gttatcgggt
    28261 tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa tcagggcacg tatgatttca
    28321 agtatattgc tgtagcgctg atcacattag gattaacaat tttcttcaac atgtggctaa
    28381 gaggttttct cggattgatt ccgattttac taggtattat tagcggatac atcgtagcct
    28441 tgctgtttgg aatcgtggat actaagccaa tcgtagacgc tgcctggttt gctgttccta
    28501 actttgagat tccctttgta caatatgaac caaagctata tcttggagca atcacaacaa
    28561 tggcacccat cgcttttgtg acgatgacag agcatatcgg ccatcttatg gtattgaata
    28621 aactaactaa acgtaatttc tttgaagaac cgggtcttca taagacgttg acaggtgatg
    28681 gattagcaca aatcgtggca gcctttgtcg gaggtcctcc tgtaacaagt tacggtgaaa
    28741 acattggtgt tctggctatc acacgtgtcc atagcgtgtt tgtgatcggt ggagctgcag
    28801 tatttgcagt cattttagga ttcgtaggaa aattaagcgc tttgatttta agtatccctg
    28861 gaccagtcat ctcaggaatc agttttatct tatttggagt aattgccgca agtggaatga
    28921 agattttaat cgagaacaaa atcgattttg ataaaaagaa aaatctgttg attgcttcag
    28981 ttatcttagt agtaggaatt ggcggactcg tctttgaagt aggcacattt acattgtcgg
    29041 caatggcatt agcaactgta cttggcatca ttttaaatct tatcttacca gaaacttcac
    29101 gaagcgaaga aaactaagga ggaaacatta tgatcatcaa atcagaacgt atcagcttaa
    29161 agcatttatt gaccgttgag gcacttactg atcaagaagt tatgggcttg atccgcagag
    29221 ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa gcgccagtac tttgctacaa
    29281 acctcttttt cgaaaatagt actcgcacac ataaaagctt cgatgtagcg gagaaaaaac
    29341 tagggctaga agtgattgaa tttgaagcaa gcacaagctc tgtccaaaaa ggggaaacac
    29401 tatacgatac agtactgacg atgtcagcat taggcgtaga cgtagcggtt atccgtcatg
    29461 gtgatgagaa ctattatgac gaactgatac aaagtaaaac aatccagtgt tcgattatca
    29521 atggaggaga cggaagcgga cagcatccaa cccaatgctt gcttgatttg atgaccattt
    29581 atgaagaatt tggtcatttt gaaggtctga aagttgccat cgtaggtgat atcactcact
    29641 ctcgcgtagc taaatccaat atgcagatgt taaaacgtct aggcgcacaa gtattcttct
    29701 caggtcctag agaatggtac gacgatgaat tcgaagtgta cggacattat atgccattgg
    29761 atgaactact agatcaagtt gatgtcatga tgatgcttcg ggtgcaacac gaacgccatg
    29821 atggaaaaga aagtttctca aaagaaggct accatcagga acatgggtta acagttgagc
    29881 gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc agcaccagtt aaccgtgatg
    29941 tagaattagc tgattcatta gtggaaggat tacagtcgcg gatcgttcag caaatgagca
    30001 atggagtatt cgtcagaatg gcaatcttag aagcagtgct aaatggaaaa gcgtaattat
    30061 aggaggatga aaaaatgaaa acactcatta aaaacggtca gatcaataca cgaaaaaata
    30121 tgacgacacc agcagaaatt tggatagaag atgggcgaat caaagcaatc ggtacaggat
    30181 tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg acaattgatc acacctggtt
    30241 tagtggacgt ccatgttcat ttgagagagc caggatttac atacaaggag acgatcgaag
    30301 ctgggactcg ctcagctgct cgtggcggtt tcactactgt atgcgcgatg ccgaatttaa
    30361 atcctgtacc tgatacggca gaaaagctga gacaagtata tgacatcatt agaaaagatg
    30421 cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa tctgcgaagt gagaaattag
    30481 ttgatcaaga agcactgatt gaagaaggtg cttttgcttt cacaaatgac ggtgtaggtg
    30541 tccaaacagc tgggacgatg tatctggcta tgaaagaagc tgcaaaaaac aataaagccc
    30601 tagtagccca tacagaagat gaatctttat tatttggcgg cgttatgcac gctggcaaga
    30661 aggcagaaga acttggtctt cccgggatct taagcgtaac cgagtcttct caaattgcta
    30721 gagatctgtt gctagctgaa gcaactggtg ttcattacca tgtttgccat gtttctacta
    30781 aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg aatccacgta acagcagaag
    30841 tttcacccca tcatcttatt ttgatcgacg aagatattcc cgaagatttc ggcttttgga
    30901 aaatgaaccc gccgctaaga ggacgggagg acagagaagc tttgatcgaa ggcttgcttg
    30961 acggtacgat cgattgcatc gctacagacc atgcccccca tggattagaa gaaaaaagcc
    31021 aaagtttcat gaaatcacca tttggtatcg taggaagcga aacagcgttt caactgatct
    31081 atactcattt tgtagaaact ggtcgattca cgttggaaca agtgatcaat tggttggcag
    31141 ttaaaccggc tgagattttt ggtttgaatg caggaacgct aacggttggt gcccccgctg
    31201 atgtagcagt ttttgatatt acacaaactt gcacgatcga taaagaagac tttttatcaa
    31261 aaggggagaa tacacctttt atcggctgga aagtaaaagg cgaaacacaa atgacttttg
    31321 tcaacggaaa acttgtgtgg cagaagggag aataaaagta tggaacgttt gcttatatta
    31381 gaagatggaa cagttttcga aggaaaggct tttggtgcag atgctagtgt cttcggagaa
    31441 atcgttttta caacaagtat gactggttat caagaaacta tcactgatca aagttttaac
    31501 ggtcaaatca ttacatttac ctatcctatg gtgggtaatt atggggtaaa tagagatgat
    31561 tatgaatcga tttctccaac atgcaaagga gtagtagtaa aagaacatgc acgtgtagct
    31621 tcaaactggc gaaaccaaat gacgctagat tcatttttga aacaaaaagg aattcctgga
    31681 atatcaggta tcgatacgcg cgcccttact cgcaaaatcc gtgaagtcgg aacgatgaaa
    31741 gctagtatag ttgatgctgg agattccttc gaacatgcat ttgatcagct gaaagcaacc
    31801 gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca aaccttatcc aagtccagga
    31861 acaggtcgga aagtcattgt cattgacttc ggcttgaaac atagtatttt acgagaattg
    31921 agtaaaagaa actgtaacct cacagtactt ccctataaca cagatgccga gacgatttta
    31981 gcgctatcac cagacggagt catgctgacg aatggacctg gtgatccaaa agatgtgcca
    32041 gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac cgatttttgg tatttgtctc
    32101 ggtcaccagt tatttgcttt agccaacggt gcagatacct ataaaatgaa attcggacat
    32161 cgaggattaa atcacccggt acgagaagta gcgaccggaa gaattgattt tacttctcaa
    32221 aatcatggct atgcagtcga tgaagccacg attgatccag aaaaactcat cgtaactcat
    32281 gtagaagtaa atgacggaac agtagaagga gtcaaacaca gagattatcc cgctttcagt
    32341 gtgcagtttc atccagatgc tgcccctggt ccgcatgatg cactacattt atttgatgaa
    32401 tttatggaaa tgatggacgc aggaaaggag cagtaaacag atgccgaaac gtacagatat
    32461 caagaaaatt atggtgattg gatctggtcc gatcatcatt ggtcaagccg cagaatttga
    32521 ctacgcagga acacaagcct gccttgcctt aaaagaagaa ggatatgaag tggttttagt
    32581 aaattctaat cctgcgacga tcatgacgga taaagaagtg gcagatcaag tatatatcga
    32641 accgatcaca cttgaatttg tatcaaggat tcttcgaaaa gagcgtccag atgctctcct
    32701 tccaacttta ggcggacaga caggcctcaa tatggcaatg gagctggcag catcaggtat
    32761 tttagaagaa ttgaacatag aacttctagg aacaaagctt tctgcgatcg atcaagcaga
    32821 agatcgtgat ctattcaaac agttgatgaa agaattgaat cagccaatcc ccgagtctga
    32881 aatcgtcaat acagtagaag aagctgtaga ttttgcaaat aaaatcggct atcctgtcat
    32941 cgttcgtcca gcctttactt taggcggaac tggtggaggt atgtgtgaaa atgaagaaga
    33001 attacgtatc gtatcagaaa atggattaag tctttccccc gctacccaat gtttgattga
    33061 acgaagtatt gcaggtttta aagaaattga atatgaagtg atgagagatt cggctgataa
    33121 tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg ggtatccata caggtgattc
    33181 tatcgtattt gcccctagtc aaacattgtc tgaccatgaa tatcaaatgc ttcgtgatgc
    33241 ttctctttcg attattcgag cacttaagat cgagggaggc tgtaatgtcc aactcgctct
    33301 tgatccacat agttttaatt attatgtgat tgaagtcaat ccgcgtgttt ctcgttcatc
    33361 tgcgttagca agtaaggcga ctggctatcc gatcgctaag ctagctgcta aaatagcgct
    33421 tggattaaca ttagacgaaa tgaaaaatcc tgttacagga acgacttttg cagaatttga
    33481 acctgcatta gattatgttg tagctaaaat tcctcgctgg ccgtttgata agtttgaaaa
    33541 cggagaacgg cttttaggca cgcagatgaa agcaactggt gaagtgatgg ctatcggtcg
    33601 gaatatcgaa gagtctttat tgaaagctgt gcgttcttta gaaatcggta cacaccatat
    33661 cgaacttcct gagttggcag acatcacgga cgatgaccta atccaaaaag tggttcgagc
    33721 acaagatgat cgcctgtttt atgtagctga agctattaga agagggtaca ccatagaaga
    33781 attagcggat ttgacaaaaa tcgatttatt ctttttagat aaactgttgc atatcgtaga
    33841 aatcgaacag cagttgaaac agcagccgtt ggatgacaag atattgaaaa cagcgaaaca
    33901 aaacggtttt acagacaaaa aagtggcgga gctgtgggaa ataaacgaag aagccgttcg
    33961 ccagatgaga aaagaaaatg atttgattcc tgtatataag atggttgaca cttgtgcagc
    34021 ggaattcgaa tcaaaaacac cttattttta tagttcctat gaatatgaaa atgaaagttc
    34081 acgatcagaa aagccctcaa ttctagtact gggatctggt cctatccgta tcggacaagg
    34141 ggttgaattt gactatgcga ctgtgcattc tgtcaaagca atccagcagg caggatacga
    34201 agcaatcatc atgaacagca atcctgaaac agtttcgact gacttttcta tttcggataa
    34261 attatatttt gagccattga ccttcgaaga tgtgatgaat gtgatcgagc tagaacagcc
    34321 gataggagta atcgttcaat ttggtggtca gacagcaatc aacttagccg aacctttgac
    34381 tgctgctggt gtaaaaatct tagggacaac gatcgaagac ttggatcgtg cggaaaatcg
    34441 tgatttattc gagcaagcgc tgaaagagtt ggatattcct caacctttag gggatactgc
    34501 tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt ggttatccag ttcttgttcg
    34561 tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt gaaaaccagc gagatttaga
    34621 agattacatg gaacatgctg tgaaagcatc tccagaacat ccagtactag tggaccgtta
    34681 cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac ggtgaaacag tactcatccc
    34741 agggatcatg gagcatattg agcgtgctgg tgtccattca ggggattcaa tggctgtgta
    34801 tccacctcaa acattatctg aatcgatcaa gaaaacgatc gaagattaca cgatccgctt
    34861 agcattaggt ttgaattgtg tcgggatgat gaatatccag tttgtcatcc atgaaaatga
    34921 ggtttatgtg atcgaagtca atcctcgagc aagtcggact gttccattct taagtaaggt
    34981 gacaggaatt ccaatggcac aaattgcgac gaaagctatt ttgggagaga aactaaagac
    35041 acttggttat gaaaacggac tttatccaga aacaaaagaa gttcatgtta aagcacctgt
    35101 cttttctttt acgaaattgc agaaagttga tacttactta gggcctgaaa tgaaatcgac
    35161 tggagaagta atgggatctg acagacaatt agacaaagct ttgtacaagg cattcgaagc
    35221 ttctggatta cacttgcctg aatatggagc cgtattattt acgattgcgg acgaaacaaa
    35281 agaagaagca ctagaattag ctaaacgttt tacagaaatt ggctatagtc tgataggaac
    35341 gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc gttgaaacag tagcgaaaat
    35401 ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata agagatggta aaacacaagt
    35461 ggttgtcaat acgattgata aagatcgggg aaatgcttct aaagacggtt tcatcatccg
    35521 aagagaagca gttgaacatg gtattccatt gtttacttct ttagacacag cagatgcgat
    35581 cgttcgagtg atggaatccc gtgctttttc aactaaccct atttaacggc gagcacatgt
    35641 taagacaaaa agacgaaaaa ccgttgcagg aggggacaga tgaaacaaga aatgatggaa
    35701 atcgttcagc aaaaacagct ggcaccaaag atttttgaaa tgacattgaa aggtgatcta
    35761 gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc tcgtgccgcg tgcagacttg
    35821 ctattacgcc gaccgattag cttgaatcat atcgaccacg aaacaaatac ttgccgtatc
    35881 atttatcggg tggaaggtga ggggactaga gtcttctcta ctctttctgc cggagaccat
    35941 ttagatgtca tgggtccttt aggcaatggt tttgatttgt ccaatcttca tacaggagac
    36001 gaagtattta taatcggtgg aggaatcggt gtccctcctc tatatgagtt atcaaaacag
    36061 ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt ttgccacaca tgaagtcgtt
    36121 tattatgaaa aagaatttaa tcgtctagga gaaacaagaa tcgctactga cgatggaaca
    36181 tacggtatgc acggaaatgt cggtaattta ttgttaggaa cgaagacgga acctacagct
    36241 gtttttgcat gtggttcaaa cgggttattg aagacagtgg aacagctgtt ttcttcgcat
    36301 caaaatgtcc agttatcatt agaatcccgc atggcatgtg ggataggggc ttgctatgct
    36361 tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa gtgtgaaagt ttgtgacgaa
    36421 ggaccgattt ttaaaatagg agaggtaatt atatgaatga gaatccttta gcggtcaaac
    36481 ttcctggcct agaattaaaa aatcctatca tgccggcaag cggctgtttc ggttttggca
    36541 aagaatacgg aaaatactat gatttgaatc agcttggatc gatcatggtc aaggcaacga
    36601 ctccaaatgc ccgctttggc aatcccacgc caagagtggc ggaaacacca tctggcatgt
    36661 tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt tatgcataaa cttcttccag
    36721 aattagagga gtacaatgag ttacccatca tcgcaaatgt tgctggtgct tgtgaagaag
    36781 actatgtaga agtttgtagc aaaattggcg aagcaccaaa tgtgaaagca atcgagctga
    36841 acatttcttg cccaaatgtc aaacatggcg gaattgcttt tggaacagat tctgatgttg
    36901 cttttcaatt aacacaagca gtaaagaaag tttcttctgt tcctgtctat gtcaaattgt
    36961 ccccaaatgt aacagacatc gttccaatcg ctcaggcaat cgaagctgga ggggcagatg
    37021 ggtttacgat gatcaatact cttcttggta tgcggattga ccttaagaca agaaaaccga
    37081 ttttagccaa tcaaacaggt ggactctccg gaccagcaat caagcctgtc gctattcgtt
    37141 tgattcacca agtagcaagt atatccagcc ttccaatcat tgggatgggc ggcgtgcaga
    37201 cagtggatga tgttcttgag atgtttatgg ctggagcaag tgctgtagct atagggaccg
    37261 cgaatttcac cgatccgtat atctgtccta aactaattaa agagctccct gtgcgaatga
    37321 gcgaactagg catcgaatcg ctggaaagat taatcaaaga agtgagagag gaacgtgaac
    37381 gatgaatcaa ctagaccaac gaccaattat tgcattagat ttttcaacta ggcaagaggt
    37441 agaagacttt cttcgatttt tcccgaaaga agaaaaattg tttgtgaaaa tcggaatgga
    37501 attgttctac caagaaggac ctgaaatcgt acgttatcta aaagaggctg gtcataatgt
    37561 atttttagat ctgaaacttc acgatattcc gaatacggtc gaaaaagcaa tgcggggatt
    37621 ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct ggcggtattc gtatgatgga
    37681 ggcggcaatg cgaggattag aagaggggac tccagaaggt ggaaaacgtc cgctacttct
    37741 agctatcact cagcttactt ctacaagtga agaagaaatg catgccgatc aactaatcga
    37801 agtttcttta gaaaaaagtg tgatccatta tgctagctgt gccaaaaaag caggattaga
    37861 tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa gaaatggcag gagacgaatt
    37921 tgtttgctta actccgggta taagaccaga aggaacagtc gcaggagatc agacaagagt
    37981 agtcacacct tctcaggcaa aaaagatcgg ctcaactttt atcgttatag gtcgaccaat
    38041 cactcagtca actaatcctt ataaagcata tcaaacgatt caaacagaat ggagctaacc
    38101 taaaatgaat gttgaacaaa gtatcgcaaa agacttatta gaaattgaag cagtattttt
    38161 aaatccaagt aatcctttta catgggcaag tggaatcaaa agcccaattt actgtgataa
    38221 ccgaatcacg atgagctatc caaaagtccg aaaagagatc gcaaaaggat tagcaagcaa
    38281 aatcaaagaa gcttaccctg aagtacaagt gattgctgga actgcaacag caggaatccc
    38341 tcatgccgca tgggtagcgg aaatactgga tttgccaatg gtctatattc gaagtaaggc
    38401 gaaagatcat gggaaaggta accaaatcga aggacgtata tttgaaggtc agaagatggt
    38461 tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg gaagcagctg aagcagcaaa
    38521 aagagaaggt gcggatatcc ttggtgttgc ggctatcttt acgtatgagt tgccaaaagg
    38581 aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg ttgaccaatt attctgtatt
    38641 gattgaagca gctttagaag atcgttatat cgatgagcaa gaattaactc tgctaaaaga
    38701 atggaaaaaa gatcctgaaa attggcagat tgaagcgcgc ttgatgaaga tagagaaatc
    38761 cgatttgtgt ctccagaatc atcaattgga atagcaaaaa tccaaaaact gattgcgaga
    38821 agcatctttt catcaggtat gaaaagaaac ttctatcgtg atcagttttt gattttttta
    38881 aaattcattt ttctaagact ggttagcaga aagaagagaa taatctcttc tcttttttct
    38941 attatatata gattttctct attttttggt aagggagctg tttcaaaaat ggtaaagagc
    39001 ggaaatgttg cgaatatcag ttatttaatg atatgatatt cggaaaaaga aatctcaaag
    39061 ggaggtaact tttatggggt tgaatgaatt tattttagga ttgaacaatt tagagacgat
    39121 cacaagggca cctggatttt ttaaatatac agagcataca gttgcagcac attcgtatcg
    39181 tgtagcatct attgcgcaag ttctaggaga cattgaagaa gcgaatggac aaccgatcga
    39241 ttggaaactg ctctatgaaa aatcattgaa tcatgactac acagagcgtt ttattggtga
    39301 tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga gggatgctgc aaacagttga
    39361 agaaaagatg acggacgaat tcatcaacca agagattcca gctgagttcc aagaagttta
    39421 tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa ggagaactat tagccatttc
    39481 agataaggtt gaccttcttt atgaatcttt tgatgaaatc atcaaaaata acccggaaaa
    39541 agtttataag gagatgttca ttgaggcagt gactacgatt aaaagctttg aacatcggcc
    39601 ttctgttcag tatttcttta aagagatatt tccggaacta ttgaaccagg aattttatgg
    39661 aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag aaagaagaaa aataaaaatg
    39721 atatgagatg cagggggaag tatagttgaa aaagaatatg gatgtcgaat ggggctattc
    39781 tggagacaaa ggtccggaaa attgggcgag tttatgtgat tggtttgcga gaggtgcgga
    39841 atttccattg cagtcgccta ttcatttgac caagggggaa gaaaccaaaa tagaaggaga
    39901 cacgctttcc tttcattatc aaaagcaacg gtttactgac aaggaattca aaaacacgat
    39961 tcatttagta ccttttgacc aactcagtta tgtagagttc aagaaagaac gctattattt
    40021 gacagatatt catttccact tgccttctga acatatcatc aacggaaaac aggaagatat
    40081 tgaatttcat tttgttcata tgaacagcaa aaaagagaat ctagttgtgg gtgtcatgta
    40141 ccagctaatg gtacaggaag gttggctgta taatcaagaa aatggggaaa gttggaactt
    40201 agaaaaacat gagcattggg taaatccgga tgtgttcttt ccggagcaaa aaagccattt
    40261 ccattatatt ggctcattga caactccgcc taccagcggg ccaatccaat ggtttgtaat
    40321 ggatcatgta ggaaaactga accaagagtt tcttctcccc ttcgatggtc agtatgctcg
    40381 accgaacaac cgaccaatcc agcccttgaa tggaagagaa atttattatt tcgaggagtt
    40441 tgagcaatga atattcagca gatgaaatac gtggtcgctg tagcaaacaa tggtagtttc
    40501 agagaagcgg caaagaaaat gtttgtctcc cagccaagtc tttcagctgg tatcaaagaa
    40561 ttagaaaccg agttagggat cacgctgttt actcgaacga atcgaggggc atatttaaca
    40621 gaagaagggc aagacttctt aaatcgtgcc gagaaaatat tggtccagct tgatacatta
    40681 gaaactcact atctaacgag tgaaaagaga gacaattttt cgatcgctgc ccagcattat
    40741 gatttcttag gtcctttgac cgcacagcta ctcgaggaat tcaaagacga gatcaaacaa
    40801 tttcggatca tggagacgac aacagcgaga gtgatcgaag aagtcaaaga gcagcacagt
    40861 gagatcggca ttatttatat gaatgagcat aatcaagctg gtatcagaag atatttacaa
    40921 caaggagagc taagtggccg cagcattgga aaattcaaaa cgcatatttt tttaaggcaa
    40981 ggacatccat tagctgaaca cgcagagatc accaaagaag aattgttatc ttatcctcaa
    41041 gttcgtttta cacaggatgg aaataatttc ccgtattttt atgaagacct tatcgagatc
    41101 cctgatcaag aaacagtgat ttatacgagt gatcgaggta ctttgatgaa tatcgttctt
    41161 gaaactgatg cttacgcttc aggatctggt attgtgattg gagggattcg cgagcacttg
    41221 cggttaatcc ctttggcaga aggcgaacta aatgaatttt atatcattta ttcggcgaaa
    41281 cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc ttactcggtt actggaccag
    41341 cagagctaag aaaagcagat gagaacgctg tttattgaac accgttgatc aaaaaagagg
    41401 ggttgtgaca gaattttgtc acaactcctc tttttgtagc aaacaattta taactaattg
    41461 ctgttgtggg aaagtacgca aatatgattc tagaaacact cattgattac ctgttttgtt
    41521 tcatagctgt aattttttct tcttctggtg tcacgataat cgtcttttgg ttttcataaa
    41581 taacataacc aggcttggcg ccatttggtt ttcggatatg ctttacctgt acaagatcaa
    41641 ccggcacttg ggcagagtat cggtattttg aaaaataggc agctaactcg gcagcttctg
    41701 taattgtttc atctgagggc ttgtcacttt taatgatgac gtgcgaaccg ggtatatttt
    41761 ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat tgtcagctgg tcattttgaa
    41821 gattgttttt accaacaaga atgagcgttc cgtcagaaga aaagtattga tcaggctgag
    41881 atggtttctt acgtttttgt ttttttaagt tcttcttttt caggtatcct tctgaagtca
    41941 attcttcttt aattgcttcg atatccattg ggccggcaat ttccagttga gataagacag
    42001 attcaagata ttggatttca tctttggctt cttggatttg ttcgccaatc agcttaacag
    42061 cgttcttcag cttttggtat cgatggaaat atttctgtgc attttgattt ggtgtcaatg
    42121 ccggatctag tgctattttg attggccgat cttcttcata ataattaggt aaaacaactt
    42181 cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag tagttcgcca tttctacggt
    42241 aattctcagc gttttcggat tctttgagtg tttgttcacg ttttttcaat ttatttttat
    42301 ttcgttttag ttcattttcg atttttcgaa tcagttctcc gccttgttgt tttgcccgat
    42361 ctttctctgc tttttcgtga taatagctat ctaataattg actgagtgtt ggataagctg
    42421 ttacagcaga tgcctgttcg gacaatacct gataagcaat cggtgtgaaa aaatctttat
    42481 tttccgtttc gtaaaaagta ggaataggct gatggctgat ggctgagaaa aattcgtccc
    42541 atacaaccat cttttcatta ggacgttcca gtaatctttt ggtcagctct tgagcgatat
    42601 cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg atctagttca gtttgggaaa
    42661 gccgctgaaa gattttttct ttctcggagc taaatgggtt caagacctct tgttttggcg
    42721 gagcaacata ttctacacca ggaagcaaag aacgataagt gttttgagag cttccaatgt
    42781 gtttgatcgc atccagtatt tttccagttt ctcgattaac taagacgatc gtactatgac
    42841 gtcccattaa ttcaacgatc aatatgatat tttgtagatc acccaactcg tttcgtttag
    42901 caaagtggaa atggatcact cgatcatttt cgatttgttc gatgtcttct aaaatagcgc
    42961 catccaaata ttttcgaagc atcatcacga agttaggcgg attatcagga ttctgataat
    43021 cgatttgggt aatctgtaca cgagcataac ttgggtgcgc agataataat aatcgatggt
    43081 ttttgccgcg tgaacgaatc accagcacga tttcattttc ataaggctga tggattttcg
    43141 agatccggcc agatagtaac gtttcacgca attcgttgac catcgcgtgg gtaaatacac
    43201 catcaaagga catggttcat tcctcatttc ttttcataag tatattcatt ataacgaaaa
    43261 aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag tctagaaaaa tcttggacta
    43321 attacataaa aaacagtgtt tctatcgttg aaatccaaat ttttaggt
//
LOCUS       DAYYDS010000013        41165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000013 DAYYDS010000000
VERSION     DAYYDS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.013"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1233)
                     /locus_tag="ACHA02_000742"
     CDS             complement(451..1233)
                     /locus_tag="ACHA02_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669114.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1298..2134)
                     /locus_tag="ACHA02_000743"
     CDS             complement(1298..2134)
                     /locus_tag="ACHA02_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8669115.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="ACHA02_000744"
     CDS             complement(2152..3561)
                     /locus_tag="ACHA02_000744"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8669116.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="ACHA02_000745"
     CDS             complement(3575..5398)
                     /locus_tag="ACHA02_000745"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8669117.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6241..6567)
                     /locus_tag="ACHA02_000746"
     CDS             complement(6241..6567)
                     /locus_tag="ACHA02_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669118.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6613..6975)
                     /locus_tag="ACHA02_000747"
     CDS             complement(6613..6975)
                     /locus_tag="ACHA02_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8669119.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7012..7227)
                     /locus_tag="ACHA02_000748"
     CDS             complement(7012..7227)
                     /locus_tag="ACHA02_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8669120.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7354..7689
                     /locus_tag="ACHA02_000749"
     CDS             7354..7689
                     /locus_tag="ACHA02_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8669121.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7765..9762)
                     /locus_tag="ACHA02_000750"
     CDS             complement(7765..9762)
                     /locus_tag="ACHA02_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669122.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9860..10135)
                     /locus_tag="ACHA02_000751"
     CDS             complement(9860..10135)
                     /locus_tag="ACHA02_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8669123.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10367..11101
                     /locus_tag="ACHA02_000752"
     CDS             10367..11101
                     /locus_tag="ACHA02_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8669124.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11140..12282
                     /locus_tag="ACHA02_000753"
     CDS             11140..12282
                     /locus_tag="ACHA02_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8669125.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="ACHA02_000754"
     CDS             complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="ACHA02_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8669126.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13773..14243)
                     /gene="sufU"
                     /locus_tag="ACHA02_000755"
     CDS             complement(13773..14243)
                     /gene="sufU"
                     /locus_tag="ACHA02_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8669127.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14230..15465)
                     /locus_tag="ACHA02_000756"
     CDS             complement(14230..15465)
                     /locus_tag="ACHA02_000756"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8669128.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="ACHA02_000757"
     CDS             complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="ACHA02_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8669129.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="ACHA02_000758"
     CDS             complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="ACHA02_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8669130.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17731..18579)
                     /locus_tag="ACHA02_000759"
     CDS             complement(17731..18579)
                     /locus_tag="ACHA02_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8669131.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18603..19289)
                     /locus_tag="ACHA02_000760"
     CDS             complement(18603..19289)
                     /locus_tag="ACHA02_000760"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8669132.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19282..20316)
                     /locus_tag="ACHA02_000761"
     CDS             complement(19282..20316)
                     /locus_tag="ACHA02_000761"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8669133.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20379..20635)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20711..21049)
                     /locus_tag="ACHA02_000762"
     CDS             complement(20711..21049)
                     /locus_tag="ACHA02_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8669134.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21051..21407)
                     /locus_tag="ACHA02_000763"
     CDS             complement(21051..21407)
                     /locus_tag="ACHA02_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8669135.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21413..22600)
                     /locus_tag="ACHA02_000764"
     CDS             complement(21413..22600)
                     /locus_tag="ACHA02_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8669136.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22791..23636)
                     /locus_tag="ACHA02_000765"
     CDS             complement(22791..23636)
                     /locus_tag="ACHA02_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8669137.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23870..24835
                     /locus_tag="ACHA02_000766"
     CDS             23870..24835
                     /locus_tag="ACHA02_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8669138.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25212..26255
                     /locus_tag="ACHA02_000767"
     CDS             25212..26255
                     /locus_tag="ACHA02_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8669139.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26317..26781)
                     /locus_tag="ACHA02_000768"
     CDS             complement(26317..26781)
                     /locus_tag="ACHA02_000768"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8669140.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27027..27974
                     /locus_tag="ACHA02_000769"
     CDS             27027..27974
                     /locus_tag="ACHA02_000769"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8669141.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28185..30257)
                     /locus_tag="ACHA02_000770"
     CDS             complement(28185..30257)
                     /locus_tag="ACHA02_000770"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8669142.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30190..30567)
                     /locus_tag="ACHA02_000771"
     CDS             complement(30190..30567)
                     /locus_tag="ACHA02_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8669143.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30696..31256)
                     /locus_tag="ACHA02_000772"
     CDS             complement(30696..31256)
                     /locus_tag="ACHA02_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8669144.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31406..32698
                     /locus_tag="ACHA02_000773"
     CDS             31406..32698
                     /locus_tag="ACHA02_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8669145.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32758..33876)
                     /locus_tag="ACHA02_000774"
     CDS             complement(32758..33876)
                     /locus_tag="ACHA02_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8669146.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33890..35311)
                     /gene="arcD"
                     /locus_tag="ACHA02_000775"
     CDS             complement(33890..35311)
                     /gene="arcD"
                     /locus_tag="ACHA02_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8669147.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35557..36678)
                     /gene="mnmA"
                     /locus_tag="ACHA02_000776"
     CDS             complement(35557..36678)
                     /gene="mnmA"
                     /locus_tag="ACHA02_000776"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8669148.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37082..37681
                     /locus_tag="ACHA02_000777"
     CDS             37082..37681
                     /locus_tag="ACHA02_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8669149.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37819..39105)
                     /gene="efmA"
                     /locus_tag="ACHA02_000778"
     CDS             complement(37819..39105)
                     /gene="efmA"
                     /locus_tag="ACHA02_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8669150.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39376..39720)
                     /locus_tag="ACHA02_000779"
     CDS             complement(39376..39720)
                     /locus_tag="ACHA02_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8669151.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39857..41002)
                     /locus_tag="ACHA02_000780"
     CDS             complement(39857..41002)
                     /locus_tag="ACHA02_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8669152.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11848 a         8652 c         7193 g        13472 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
     6121 cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact
     6181 ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa
     6241 tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg
     6301 atagtactca atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta
     6361 tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta
     6421 ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc
     6481 tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa
     6541 cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc
     6601 ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac
     6661 ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat
     6721 aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat
     6781 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
     6841 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
     6901 ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca
     6961 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt
     7021 tactttatta aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag
     7081 catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
     7141 ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta
     7201 tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa
     7261 tatgatgtat ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa
     7321 cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc
     7381 attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc
     7441 tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc
     7501 ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc
     7561 gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt
     7621 cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg
     7681 aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa
     7741 gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac
     7801 tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc
     7861 tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa
     7921 aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag
     7981 agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt
     8041 atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag
     8101 atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg
     8161 tttttccttg ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc
     8221 gttaaaacat atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc
     8281 cagaagtgga aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc
     8341 taatcgatag acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa
     8401 cctatttagg tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa
     8461 tcctataaaa gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc
     8521 atagtaagaa ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc
     8581 cttactatca tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc
     8641 ctctagataa ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa
     8701 aagtactttt gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc
     8761 taatagccaa ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc
     8821 aataaaattt tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt
     8881 tttactggta aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt
     8941 tttacttgac ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt
     9001 aaaagacatg tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt
     9061 gtcgtcattt tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc
     9121 tgaatcactt tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat
     9181 atctaacatg ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa
     9241 cattctattc gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc
     9301 gtaaacagca accattttac catagtcagt aattgcttct tttaatagaa agacaatctt
     9361 gtatttttct agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg
     9421 aactaaattg atagataaaa ccttttctac gcttaagtat ctactatcat ctttactcaa
     9481 atctactacc ttccccttct catctttaac agtgttatcg acatccagaa taattactct
     9541 ggtttccaca aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga
     9601 ttgtgatgaa tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa
     9661 acacctatct cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc
     9721 ttgatctaca taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata
     9781 aaattctgtt aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt
     9841 tttagtctta tttgtttact caacctttat accgttccat gaaactatca atatcagatt
     9901 ttttaaatct aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt
     9961 ttctaatcgt tcctactgat acacctagat attcacttgc ctgctgatag tccatatact
    10021 ctagagtgac tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt
    10081 tcacttgttg ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc
    10141 cccctccttt cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc
    10201 aaaataaaag tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc
    10261 gacatacaca taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa atttatttgc
    10321 aacacccctt ttttgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc
    10381 accaataaat tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa
    10441 gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga
    10501 caaaaaaact atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc
    10561 ccgaattgct gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga
    10621 atatattttg ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc
    10681 agataaaaac ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga
    10741 tacatggctg gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa
    10801 gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga
    10861 attattcatc aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag
    10921 tatagaagaa ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat
    10981 gtcttatccc gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac
    11041 gatttcatca attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta
    11101 gtggtaagta ctccttgtaa actaagagga gatgatttaa tggcaaaaat atcaaagtac
    11161 aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga
    11221 actggtgaaa aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa
    11281 gattatgacg agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg
    11341 acatttgaag acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca
    11401 acaatcgcaa tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt
    11461 aaactatcga atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa
    11521 tatgaaagtt acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca
    11581 atgcaatata ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat
    11641 gagaaaaatc gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa
    11701 gattttggta acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga
    11761 aagagtgaag tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat
    11821 gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca
    11881 agagcttcta gacgagttat ctctttagat actgagactc tttctatcct caacaactgg
    11941 aaattacagc aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa
    12001 catgttttta caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta
    12061 agatatattt tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca
    12121 catgctagtt tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg
    12181 catgaaaata gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag
    12241 aaaacaggtc aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct
    12301 ggtcatttct agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct
    12361 agctcctata acaggtttac ccaacactgc cttccatctc ataactgatc aatcgattga
    12421 gttcgactgc atattccatt ggaagttctt tggtgaatgg ttcaacaaag cccatgacga
    12481 tcatttcagt tgcttcagat tcggtcaggc ctcggctcat cagatagtag agttgttctt
    12541 ctgagatttt tgagactttg gcttcgtgtt ctaaagagac ttggctgttg tggatctcat
    12601 tgaatgggat cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca cattcgatat
    12661 gagagatcga acccgcactg tttttggcaa aggttacttg tcctcgatag tttacttctc
    12721 cgccgtcttt tgaaattgat ttagagacga tagagctaga tgtatttggt gcattgtgga
    12781 tcatttttgc acccgtatct tggatttggt ttgctcctgc aaaggcgatg gagagcatgg
    12841 tgcctcgtgc accttttcca tctaagtaaa cacttgggta tttcatcgtt gtttttgcac
    12901 ctaggtttcc gtcgatccat tcaacggttg cgccttcgta tgcttttgct cgtttggtta
    12961 ctaagttgta cacattgtct gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt
    13021 ttcttgtaaa gatttctacg atggcggcat gcaggctgtt acttgaataa gtaggtgccg
    13081 tacaaccttc gacatagtgg atgcttgctc cttcgtcaac aatgatcagc gtacgttcga
    13141 attgccccgt gttttccgca ttgatccgga agtacgtttg taatggaaca tcgactttga
    13201 cgccttttgg cacgtaaata aaggtcccac ctgaccatac ggctgaattc agcgctgcta
    13261 atttgttgtc ggttggcggt acaagttttg aaaagtactc tttgaataat tcagggtatt
    13321 ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa cttttcgaat tcatctttca
    13381 tattatggta cacgacttct gattcatatt gtgcggaagc accggctaga taagctcgtt
    13441 cagcttctgg tataccaatc ttttcaaatg tatctttgat tttttctggt acgtcttccc
    13501 agtcacgagc gggtttgtca cttggttttt ggtaatattt gatggcatcg aaatcgatgt
    13561 ctgaaaggtc ggggccccat gtttgcattg gcattttgtt aaatgtttct agagatttca
    13621 gacggaattc aagcatccat tctggttcgc ctttgattct tgacatctca cggatcactt
    13681 cttctgtcaa gccttctcct gtactgaaaa ccggttcgac atcatcgtga aacccgaatt
    13741 tatattcttc taattctggt acgcccatgt tctcactcct cttccttgtg gtaatgatgg
    13801 aaagctgcgg taccatcgtt ttccattgct tttcctagtg ctttccaggc aagtgtcgca
    13861 catttgatcc gagcgggaaa tttagctaca ccaccaagca tagcagcatc gcctaattgg
    13921 tcgggatctt tgacttcttt tccttggaca agctgtagaa agtctacagt taaatgttct
    13981 gcttcttcta ttgattttcc gatgacggcg tctgtcatca tcgaggcact tgccgtactg
    14041 atggagcagc cactgccgtc aaaggcgata tttttgatga ttccgtcttt tacatcgact
    14101 tgcaattcga tcacatctcc acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca
    14161 gtcaagcttc cacgatgatg cggatgggaa gaatgatcca agatcacttg acggtataaa
    14221 ttgtctaatt tagatagtcc catgttggaa aaactccttt gttgccatga ttgcttcaac
    14281 taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga
    14341 tacttcaagg tacttcaata gcggttgcgc acaatgatga cctgctctga cggctacccc
    14401 ttccatgtct aaagctgtcg cgacatcatg gggatgtaac ccatccaaat tgaatgcaac
    14461 gactcctgtc cgaaaattcg ggtcttgtgg accataaaca gtcaatccat caattgcttg
    14521 taattttggc aacacataag cggctagttc ttcttcatat cgatggatat tgtccatacc
    14581 gagtttttgc aaataatcga ttgcatggcc taatgcgata gcgccggcga tatttggtgt
    14641 ccctgcttcg aatttccaag gtagttcttt ccatgtactg tcgtataatt cgacaaaatc
    14701 gatcatttcg ccaccaaatt caacaggttc catttcttct aaccattttc gtttaccata
    14761 taaaacacca atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca
    14821 gtctagtgct tggacatcaa tggccatgtg cggggcagat tgagcaccat ctactacgat
    14881 gaccccgccg ttttcatgga caagttcagc taattcttga acaggattga agacacctaa
    14941 gacattggat acatgagcaa tggagacgat tttggttttg tccgtaatct ttttcgctgc
    15001 atcctccata tccaggaagc cttcttctgt tagttcgata tatttcaaga cagcacctgt
    15061 tcgttttgcc aactgctgcc acgggataac attagaatga tgttccatgt aagagagcac
    15121 tatctcgtct cctgctttga tatagcggtc gccgaaggtt ctggctaccc aattcaagct
    15181 tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct tttgcatgga tgaacgcccg
    15241 tactttttct cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg tatggacacc
    15301 gcgatgaaca ttggcgttat cgtgttcgta atagtacgct aatgcctgga gtacttgttt
    15361 tggcttttgg gtcgtcgctg cattatccag ataaactaaa ggttcatcat tgacgtcttg
    15421 aaataagata ggaaaatcat tgcgtagctt tttagcatcg atcatccatt caacttccct
    15481 tcgattactt cgactaattc tttccgaact tgttctacag ggattgctgt taagacagaa
    15541 ccgaggaatc ctcgaatcac taagcgctct gcttcttctt tatgcaatcc tctactcatc
    15601 agatagtaca tttcttctgg atcaactcgt cctacactgg cagcatgtcc agcagttact
    15661 tcgttttcat cgatcaaaag aataggattc gcatcaccac gtgctttatc agaaagcatc
    15721 aagacacgac tttcttgttg ggcatctgca ccttttgccc ctttcaagat gtggccgata
    15781 ccattaaagg tcaatgttcc tttttctcgg atcactccgt gttgcaaaat atgcccgatc
    15841 gtatgagggg ctttattggt tactcgtgta tcgattcctt ggatttgtct gcccgagctg
    15901 attgccacga ttttgacttc tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca
    15961 gcgatcacat gaccgtcatt catgacacca attgcccagt cgacagaagc atctcgtaaa
    16021 atgtgcccac ggcgattcat ataagaagta atattttctc ctaattgatc tacggctgaa
    16081 tatttgattt ttgaaccagc tttagcgatg acttccacga tgatattacc tgatgatttt
    16141 gctacttgtt ctttggttgt ctggaaacgt tccagataag aaaattcact atgttcatct
    16201 gccacgatca acacatgctt gaagaagtgc tcatcggatg cgccatcttg aatgaataag
    16261 gactcgagtg gttcatcgat cacgacattt ttaggcacat acaggaaaat accgctgttc
    16321 ataaaagcta catggagtgc agtcaattgg tcttcgttca tgtttactgc ttttttcata
    16381 taatattctt ggactagttc tggatattcg atcattgcag tgaacaagtc ggtaaaaatg
    16441 actccttttt ctgctaattc cacaggcagt tgttcaaaaa tcgtccatga accttgttgg
    16501 acaagcacag gattgtcttt cattgcatca aatgccggaa tcgtgcctgt ttcactgatg
    16561 gtttgtgttt ctttcacatc aaataacgac cagcgatgga atttcacccg atcgatatgc
    16621 ggcaatggca gttcatctgc tttcgctagt gcgtcttgac ggaaggttct catccaatca
    16681 ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa gataatcgag ccagtttttt
    16741 tctttcattc ttgctccccc ttacgcttct tcttctttat agtcgattcc taattcttta
    16801 ctgataccag cgtagccttc tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg
    16861 actactcggc cttccatcat gatgtgaacg acatcaggtg tgatatagtt caataatcgt
    16921 tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc gcatttcatt tactccttta
    16981 gcaacgactt tcaaagcatc gatatccaga cctgaatcga tttcgtctaa aattgcaaat
    17041 gtaggttcta acatcaacaa ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa
    17101 ccttcgttca gataacgttc agccatttct tctggcatat ttagtaaagc cattttttca
    17161 tctaactttt tcaaaaatgc cataacagaa attttgtcgt tttcatcacg tttcgcattg
    17221 atcgctgcac gcatgaattc cgcattcgtg atacctggta tctcacttgg atattgcata
    17281 gctaaaaata agccaagtcg cgcacgttca tctacttcca agtctaaaac atttttcccg
    17341 tcaaacaaaa tttcgccttc tgttacttca tagtttggat tgcccatgat agcggcagat
    17401 aaggtagatt ttcctgtgcc gttaggtccc atgatggcat ggatttctcc ggttttcatt
    17461 gttaggttta cgcctttaag gatttctttt tcctcgatcg acacatgtag attttttatt
    17521 tctaagacag acatgcatta tacccccaaa ttctctttcg ttctcactca ttttagacta
    17581 attgagaatg aagcgcaatg accttcctta ctaaaatcaa atatctttta tttattatta
    17641 aacaattttg atttctaaat tagaataggt ataaaaaaga tgagaaataa gccattacag
    17701 acttatttct catctttttt gttccatacc ttattttgca tcagccttta ctggtttaac
    17761 agagccgccc catttgtctg tgatccattc ttggacttct ttgcggtgaa gcacttcaat
    17821 cagttttttg attttgtcac tgtcttcatc acctttacga gtcgcaataa tattcacgta
    17881 aggagagttg tccttttcaa gcaatacggc atcttctaat ggattcaacc ctgcgccgta
    17941 agcgaagttt gcgttgattg caactaggtc accttcatca ttttggtacg tagaagctaa
    18001 aagtgatggg tcgatcgtat ggttgaactt caagttctta gggttttctt caatatcatc
    18061 gaatgttgct gtttccagat ctactccatc tttgactttg actaagccag catcttgtag
    18121 aattgtgatg atgcgtcccc agtctgattc agaatttgaa gtaatgattg ttgcaccatc
    18181 ttttaattcg gaaacatctt tgactttttt agagtacaag cccattggtt cgatgtggac
    18241 agcaccggca ttgacaaagt catatccttt ttctttgatt tctttgttca aataaggaat
    18301 atgctggaag taattggcat caatgtcgcc ttcttccaaa gattggttcg gtaatacata
    18361 gtcatcgaat ttgacgattt caagatcgat tccttcatct ttcaataatg gtttcacatg
    18421 ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc
    18481 tttatttgct gaatctttgc tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag
    18541 caaaagcgct gctgcaaatc caaaaatttt ctttttcata tcctacatcc ccctatatcc
    18601 tattatcttt tgtctgtttt cttagttaag aaatcaccga ttccttgaac gataaatacg
    18661 attagtaaaa taattaaagt cgcaacgaat gtcactgtta gattaccacg ctggtagcct
    18721 tcctgccaag caagacctcc aagtccacct gcgccgatcg cgccggccat cgcagtaaat
    18781 ccgatcatcg agatcgttgt aactgttaat ccagaaatca atgcgggaat actttcaggt
    18841 atcaagacct tccagatgat ttcccagtaa cttgctccca ttgcatctgc tgcttccagt
    18901 acaccagggc tgacttcacg aaaagcaatc tctactagac gggcataaaa tggtgctgcg
    18961 gataaaacaa gagctggaat ggctgcttgc gcacctagca tagtgcctac gattgcttta
    19021 gtaaatggaa tgatcaaaac catcaggatc ataaatggcg ttgaccggaa aatattactg
    19081 atgatcgata cgattccata aatgattcga gcatacggtt tgtttttgcg tccaatagag
    19141 taaaggatca atcccaaaac taatccgatg atcgtcacta agaccattga gattaatgtc
    19201 atatatagtg tatcaaggat agcctgattc atcgtttccg ggttgatttg tttaaaatct
    19261 agatatgttt ctgaaaagct tttatccacg atggatcacc tcaattccta ctccttgttc
    19321 tttgatgaac acttctgctt gatctaattc tttggtttca ccagtcatca agacagtcaa
    19381 tgaaccaaag gaaccttctt tcacttgttt gatctttcca tacacaacgt tgacatcgat
    19441 gttatagcgt cgaatcgctt gggaaatgac tggctcgttg gcatttcttt cgctaaaggt
    19501 caaagttgcc aaacggccgc ttggattttc tttgatgagt tcagctaata gttctgtcga
    19561 atcagcttct ggttcgatgt cttgttgtac aaagcgtttg gttacttctt ttttcggttg
    19621 tctgaagaca gtcaatacgt ctccttcttc tacgactttc cccaactcca tcacagcgac
    19681 tttgttgcag attttgcgga tcacattcat ttcatgtgtg atcaagacga tggtcaaatt
    19741 caatttctta ttgatcgcta acaataagtc taatacttct tctgttgttt gcgggtccaa
    19801 tgcgctagta gcctcatcac aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat
    19861 accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc
    19921 taacccgact aaacgcaaca gttcttctgc ccgttcttta cgtttgctct ttggtacgcc
    19981 agctaattcc agcggcagct ggatattttc aagtacagtt cttgaccata acaggttgaa
    20041 atgctggaag atcataccga ttttctttcg gaatgtacgt aattctttgt tttttaattt
    20101 tgtaatatct tgatcgttta cgatcacttc gccgctcgtg ggaagctcaa gtccattcag
    20161 gagacggact aatgtacttt ttcctgcgcc agaatagccg acgattccat aaatatcccc
    20221 tttttcaacg tttagtgaaa cgtcattgac ggcttggatg tttccgtttt tcccattgaa
    20281 tgtctttttg acattcgtta gttggattaa tgacatgcca ttcacatctt tctcttatga
    20341 tttttcaaac aaaaaagcct tcgccctctt gccttaagca aaaggacgaa agcttgaact
    20401 tccgtgttac caccttgatt taccgttact tcgcaataac agcctcttcc agtacttgga
    20461 cttagcttct ttccgtatac tgtcgcgctg taacgggcac tcccgtagta agtttgctta
    20521 cgctcgctta ctctgctcta agaccatctt ccatctcatt ttcgttaccc attttcagca
    20581 aacagggctc tcttcaaacg tccgtgacat gtactcttct tttcaaagct ttctcgtttc
    20641 atataggatt gcttatgatt atagcaactg gaagaatcag tgtcaacttt tttcaagtga
    20701 ttttctgatt ttataattcg tcaaattctt ttggatcaac atctttgaaa cttaagatcc
    20761 acgcattcat ttcatctgaa tcattcaaga tattgatatc ttcaccgatt ttatcattaa
    20821 cagaagaaac tgtacccgtt agcgggctgg caaattcatt gaccgctttt tccgcttcaa
    20881 cttcgatcag aggttcatct ttttttagcg attgtcctgc ttttggcaga ttggcaaacg
    20941 tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc aacacagtat tcttttccat
    21001 taaataagat ccaaaggttg tcctttttct tcaaatgctt gcctgccatt ttatatcagt
    21061 tctccttcat aaattttttc attgaatcca attgcaacaa gctggtcctc tcgtaagatc
    21121 agtggtcgct tgatcagcat accatctgtc gataagactt ggctcgcttt tgaagccgta
    21181 aactcgccta cttgatcttt cagtccaagt tcccgatatt tcatcccgct tgtgttgaac
    21241 aggcgacgga ttggtacttg actcgtttct agccaagtct caatcgttga ggccttcggt
    21301 acttctttga tcatatcaac ggtttgataa ctaacgccat gctggtctaa ccacgctttg
    21361 gcttttttgc atgttgaaca tttcgggtac cagtaaaagg tgtacaattt tcttacctcc
    21421 ttgaagatct tgcaggtgct tcattttggt atctcatcga taatatcaac ccgataccga
    21481 tcatgttccc taaaatcgag gatcctccct gactaatgaa aggcaatggg atccctgtta
    21541 aaggaagtaa tccgatattt gctccaatat tctcaaagac atggaacagc agcatcatga
    21601 tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt atcgaaacac acccgaatca
    21661 tacgatagat taaaataaaa tacaagaaaa tgacgaatgc accaccgata aagccgaagt
    21721 tttcaccgat aacggtaaaa atcatatcgg actcacgaac aggcacatag atattgctta
    21781 cgttgaatcc tttaccaaac aagccaccgg taccaattgc caaaagtcct tgggccggct
    21841 gataactcat tcctgacgta tcatggaaag ggtccagcca tgaatcaata cgtgcaaact
    21901 gataagattt gaatccaact ttataaagca agtctcttcc gccttcagtc gtaaccaaga
    21961 agatcgttcc tgctccaacg actactgcca agattactac aggtgcgatg atgcgccaag
    22021 agattccaga catcaggaaa atcccgccaa agatcgcaag aaagacaagc atcgttccaa
    22081 agtcatcttg ggccatgatc aatgctaaaa ccggtattgt cacgatcaag agcttcccaa
    22141 tcagccaaaa gtccgatttc atcgtccgtt cttttacttg cgtattatgt ttagttacga
    22201 tcagtgccat catcaagatg tacgcaatct tcatcaactc tgctggctgg aaagtaaacg
    22261 caccgaatcg aaaccagttt ttagaccctg ttgaagcagc taaattctga tcatagaatt
    22321 tgagcaagag tcccattacc actaacccca aaccataaag gtaaggagtc agtttccaga
    22381 tccatttgga attcaaatgc atgatgacaa cgattgctgc cgctccaact aaataccaga
    22441 caacttgcat ccctacgcct ttcaagactg ctggattgtt cggatcatgg ctaagtgcta
    22501 catataatga gagcagacca atcaaacata acagaaagac tggtaaaatc acgccataat
    22561 caatacgatt atcgttcgaa gcttttaatt ttttttccat gtttcatccc ttttcttaaa
    22621 aaccatactg ctgattatta tagcggtcca gttagataat ggaaagtctt tccatagaca
    22681 tttgggaaaa attaactttc cgtttgtgca gttgcttgag caaatgcagt acaaaagcct
    22741 actgcttatc tttagtgtat catagaactt tttttgatac atgcaattat ctaccagctg
    22801 agattctcct cgctgtgaga agtttctgtg ctttcattgt atcgaaggat ctcttcggca
    22861 atctctagca gctgatcaga aaccagattg gttcgttgga tgaaataaaa atatcgatgg
    22921 aaattcaacg gagtcattgg cacattttct ggaacagagc gcttagacaa aatcgagcga
    22981 ccgattcctt ccttcagcaa cgcaacaatt acatcgttac ttttcacgat catcttctta
    23041 tcttggatat tcatctcttc aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct
    23101 tctcttacaa gccacaattt gctttttggg tccccagcga tgactagctg gtcttccatc
    23161 aaagagtatc tttgtacgcc ggaagtctct aatggctttt cgataaaacc aaaatcggat
    23221 tcatgtttcg ctacttgttc taatgattca tgagaattcg tgatttcgat ctcaaaatgc
    23281 actttaggaa accttttgat cagatgtttc atcaactgag gcaaaagata gacagcaaag
    23341 gtgtgggaag caacgatccg acatacttct tcttcatttt gttcattgag caatttttgc
    23401 tgagtttctt cccattcttc taacaaaacc agtgtccgtt catataataa gtcggcttgc
    23461 ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa gatcgatatt caattcccgc
    23521 tccaactgtt tgatttgatt cgagactgct ggttgagata aaaacagtac ttctgctgct
    23581 ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg ttaataactt aaacatggca
    23641 ccacttccat aactttttat gataattact ataaaaacta tttgatttaa atatacaaaa
    23701 aaccatctat aatagaaatg tacaacaaaa cagttgctga tccaactagt aacttagatg
    23761 aagacaatca gctgtctgct tgctcgtaat catacattcc tattcatttt acaatgtatt
    23821 cgttcatctt ggtactaaaa tctattaaat aaaaaggaga atattatata tgaagaactt
    23881 tttgaaaatt attccgatcc cgatctgcgg gttgatcctt gggttagctt cccttggaaa
    23941 tctgtttaaa gactatcacc atgttgcttt aggcaatcta actggcggta tctctatgat
    24001 tcttatgata ttcatttttg ttaaattgtt tatcttattc gaacacacga aacaaacgtt
    24061 gcatgatcca atcatcgcct ccgtttcacc aacttttacg atgtcactca tggttatttg
    24121 tacttatttt gtttcatatg aaagtattgc accaattgtc aaattcattt ggctgtgcgc
    24181 tgttatcttc caagtgatcc ttgtctttta tttcaattat caccatgtta tcaaagctga
    24241 tctttcaatt gaagcaattt atccaagctg gtttatcatt tacgttggat ttggggtcat
    24301 cactgtaact gccgggaact tcttcccact tattggaaaa attttctttt ggatttcatt
    24361 gatctgctat gcgttcttat tgccgatcat catcaaacgt gtctttttcg taagaaatat
    24421 ggctcatccg acacgtccgc tgattacgat cgttgcagca cctggttctc tttgcctaac
    24481 aggttatctt aaaaactttg ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt
    24541 atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt
    24601 ttatccaagc tatgcagcct ttacattccc gcttgttatc tctgcaacag ctatatttac
    24661 tactgttcat tacttcaaca gtttaggtat ctatagtagc gtattggatt tcttaaaagt
    24721 tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt cttgctcatt acgtacgttt
    24781 cttaattaaa gaacatagca aacatggaaa atttgtttct aaaaaaatcg cttaataatc
    24841 gattcaatag gttgtgaaaa agctgttctg catcaagtaa taagaacggc caaacaaaaa
    24901 cagctgttca tgtttttgta ctggttgggc ttattaccgc agatgcaagc ttttgaacac
    24961 cgtttaccaa gaggtcgtaa aagaagtggt cagcttcaag aaataagaag gaatttacga
    25021 aaattgcttc tcaaattttt gtgaaatttc ggcttatttc cgtagaagct acttctggaa
    25081 caccgtttat tcgaaacaag aggtcgtaca agacttttgt cacgacctct tgttcttttg
    25141 tcaaaatact ggtttagtgc tactatgttc gagtatgctt ttagtattta ttgacgaaag
    25201 gaaccgatca aatgaaatca ttgtcacatc caataaaatc tgtaaaggat tcagcggtcc
    25261 gcgaaatctt acccggttta ctgctttctt ttgccatcgc tgccttcagc aggcttcttg
    25321 ctatttatct gccacagtta ggtggagcga ctattgctat tttattgggt attttattag
    25381 gaaatacttt ttttaaacag cctgttctag caatcggcac aaaatttgct gaaagcaaac
    25441 tattagaatt ctctgttttt ttactaggtg caactgtcac agctcaaacg attgctcaaa
    25501 ttggtgtaaa aggaattgtt tttgtgttac tgcaaatgtc agtcactatc atcggtgctt
    25561 acttgattgg taaaaaactg caattcagcg atacttcttc tttgatgatg gctggaggaa
    25621 acgctgtttg tggttcttct gctattgctt caatcgcccc ttccatccat gcgaaagagg
    25681 aagaaaaagg acagatcatc acactagtca atctactggg tactgtgatg atgttgactc
    25741 tcccttttct tggattacag ttgtttggtg gaaatctcat tgaaaaaagt gcacttttgg
    25801 gtggtacact ccaatctgtt ggtcaagtag tcgctggggc aagcttactt gacaattctg
    25861 ttgtcgaatt cgccatgcta tttaagattc tacgtattat gatgctcgtt gttgttgtct
    25921 atctgtttga aaaattcatg cacaagaaac aaccagctct gtttgaaacg ccaactgata
    25981 cgcagaaaaa acgaaaatta ccgcttcctt ggtatgtatt agggtttgtg atcttttgtt
    26041 tggtcaacag cttgttttct ttacctgtag ttattgacca atcggcacat tttatcagca
    26101 gctggtttga gatcactgct ttagcggcaa ttggtctgcg tttagacttt gcaaaattca
    26161 tgaaagaagg tacacgcttc ttatcttatg gattagctgt cgggcttctc caaacagtga
    26221 tggctttact atttatttgg ctgatcaaag tttagaatga acatgaaaaa acagttgtga
    26281 ttctcgcatg gaacaatgtc cgaatcacaa ctgtttttaa ttttgcagat atccttctaa
    26341 caacaacaat tggcgtttga tttcaacccc atctattgcg tttcccatat atcctgtcag
    26401 ttttccattt tgcccgatca ctcgatgaca aggaataacg attggcaatg gattggttcg
    26461 atttgcctgc ccaatcgctc gcacagcttt cggactatca acagcaacgg ctacttcttt
    26521 atacgttcgg atttctccat aaggaatggt cgacaaagct ttccagacat tttgctgaaa
    26581 aggcgtacct ctctttatag ataaaggaac atcaaattct tttctttttc ccgcaaaata
    26641 ttcttcaaac tgttcttgtg tgcgcgttaa aattcgctga ttcgctgatt cgctttctga
    26701 aattggctga aaagatacag cagtcaatcc ggattcatct gcatctaacc ataaaaagcc
    26761 gataggtgtg tgtattttca ttttgtttgc tcctagtcaa tagactggaa cttcctcctt
    26821 tttctctatc caaaactact tgccaactaa taaaaaatgt tcttaaaagt ttgataaacc
    26881 gaggaaacat ggtaaactaa atacgttagt ttacaaaaag aatacctgtt cttttacgta
    26941 aaatcaaatt ttaaaggtgt tatttaaggt ttattaaatt ttgaatgtaa accatatata
    27001 aacatacgat tgaaaggata gaaacgatga aagtatcaat tgttgtccca tgttttaatg
    27061 aagaaaaaac tgttcctctt ttttttgatg aggttgaaaa attcaaaggt gcccatgagt
    27121 tcgaatattt gtttattgac gacggatcaa aagacggtac gttagcagca atcaagaagc
    27181 tagcctcagc ttatccttat gtccgctttg tctcattttc cagaaatttt gggaaagaag
    27241 cggctctgta cgctggattg caagcttcta caggagattt agtgaccgtg atggatgttg
    27301 atctgcaaga tccgcccgaa ctgcttcctg agatgatcaa ccggatcact acttctgata
    27361 tcgattgtgt cggcacccgg cgttcgactc gtgaaggcga gcctgcgata cgcagtttct
    27421 ttgcgaaaaa attctaccag ttgatcaata aaatcagcga tacagaaatg gtcgatggcg
    27481 ctcgtgattt ccgcttgatg actcgtcaaa tggtggatgc cgtcctagaa ttaacagaat
    27541 acaaccgatt ttcaaaagga ttatttagct gggtcggatt taaaacagaa tatatttctt
    27601 atgaaaaccg cgaacgtgtg gctggcgaaa catcttggtc cttctggaaa ttattcaact
    27661 actcgattga tggtatcgtg aacttctcgg atgcaccgtt aacgattgct tcatttatcg
    27721 gcgctttgtc ttgtgtcggt tctggtattg caattttgtt cattattttg cgggcattgt
    27781 tgtttggtga tcctacatca ggatggcctt ccatggtctc gatcttcctg ttcattggtg
    27841 gaatccaatt gttatgtctt ggaatcatcg ggaaatatat tgggaaaatc ttcttagaaa
    27901 cgaaacagcg tcctgtttat atcataagag aaacagaaaa agtgaagcca aatgaattga
    27961 agcctcacca ataatatacg tcaaatgaag taaacatttt tttaaatgaa aaaggactgt
    28021 gacaaaagtc ttgtcacagt ccttttttcc gaataaatgg tgttccagaa gtagcttctt
    28081 cggaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
    28141 ttcttgaagc tgaccacttc tatcacaacc tctttcatat cttattactt cttcattgct
    28201 gcttttagtg ctgctgctaa tgggctttct tctggttctt ctgcttgtgc atattttttc
    28261 agcaagcgtc gttcttcatg tttggtcatt ttcttcttct gctctttttt atcaagcaat
    28321 ttttctgtcg ttccatcgta tttacaacgg aaataagcac cgtttttacc ttcgatgatt
    28381 tccattttcc ggtgacattg cgggcatcga tgattagaga cttttggatc ttttcttctg
    28441 cggtagttgc aatctgaatt ggtgcagaca tagattttgc cgtcacgtgt gtttttctcg
    28501 cgcagattgg ccccacattc tgggcatttc ttttgggtaa tcgagaaatc ttgatattct
    28561 tgtttgcttg atttgatctc attcaccaag cgttttgttt ctttttcgat tccattcaag
    28621 aaggttcctg cttgtttctt tcctgcagca atagcttcaa ggtctttttc ccagctttct
    28681 gtcaaatctg gtgttactaa agagggattt accaatacta ataattgctt acctttagga
    28741 gatacttgca gtccttgagg ggtacgttcc atcagttcag atttgattag tttctcgata
    28801 atctctgcac gcgtagctgg ggttcccaga ctgtattttt ccatttgacc aagcaagctt
    28861 gcttctgaca atggtttagg cggtgcagat agttcttttt tgatggcgaa atcgggattg
    28921 attctcattc ctttttccca tttgactgtt tccgtctcag tttcagtttc gcctttccat
    28981 cctgcttgga cgacacgatt ttgccgaaaa atgaatcgtt ctttaccaaa ttcaaccgta
    29041 acttttgttt ggctgacttt atggggttga gcaaacagtc ctaaaaaacg agagacaatc
    29101 atttgataga tttttcgctc ttcattactt aatttttcat aacgtgctct gttttcagtt
    29161 ggcaaaagtg catggtgatc ggtcacttta ctgtcttgga atacttttgt ctgttggacg
    29221 atggctccgt ttttcaaata ctgcttgacc tcaggagcaa agtcgctgac tgcttgcaat
    29281 cgttctttca tcgtactttt catgtcattt gtcagatatt tgctgtctgt acgcggataa
    29341 gtaacgatct tatgggtctc atacaaactt tgtacaagac ttaatgtttt ctttgcagaa
    29401 tagccatagc gttgattggc ttctctttgg atctctgtta aatcataagg caatggcgca
    29461 gattccgttt ttaatttttc ttggacttct gtgacagttc ctttgcttgt actgatcaat
    29521 tgaacaagtt tttgtgcttc ttcttgtgtt tttaaagcaa attgattttt ttgaagcatt
    29581 ttggcttgtt cttttccagc ttgcaacgtg atcgtgaaat acgtttgtgg acggaactgc
    29641 tcgattttct tttcttggtc tctcactaat gctagtgtcg gtgtctgcac acggcctgct
    29701 gataaattat ccgcgtactt cacagttaag gctctggtca cattcaatcc taccagccag
    29761 tccgcttttg cccgggcaag agcagattca tacaatgcat catattcttt cgcaggtctt
    29821 agttgcttga atccagtttt gattgcttta tctgtttgag aagagatcca taaccgcttc
    29881 actggtttat tgaaatgcac atactctaag atccagcgag caacaagttc cccttctctt
    29941 ccggcatctg tcgcaatgac tgcttctgag acatcttttc gctgagccag ctgcttgatt
    30001 gcttttaatt gatgtcctgt tttaggaagt ggtttgattc ctagtttttt agggatcatt
    30061 ggcaacgtgt ccatttgcca gctctgccat tccttattca gatcttccgg cattttcaat
    30121 cctaataaat gaccaagggc ccaagtaacg atcaccttgg gtccttcata ataatttttg
    30181 tgcttttgat tagcaccaag caccttgctt aggtctttcg caacgcttgg tttttccgct
    30241 agaattagtt gtttcataaa attcctttct gctttctact gtgatgggct gatgattttt
    30301 caacaacgca agtcccttgt attgctgtaa atcatcgtca cactcttcac accagcgctc
    30361 atctccgtca ttccaaaagg cgctgagaaa gttacttttt gcattgatat attcataatt
    30421 ttgatggatt tcgttccatt gtttttcgta cgcttgtcgt gcttcttcga aagactcgaa
    30481 tgtttcttct tcttcaatat cttcttccca gtcttcaaaa aaccaccaag gctcattgtc
    30541 cccgaacatg gtaattactt gatacatact cgtgctcctt ctttcagctt ttctagactc
    30601 atcttagtca cttttcaaga aagttgcaag aattttctct tataaaaggg tcatttaatg
    30661 aacatcttaa aattgttaca aatgaatctg attgtttaag gcaaagtagc gataaagtta
    30721 ttgatcatcc aaaaatagct gtcttctgga tcgattgttg gttcttttgt aaagtatcct
    30781 aaaaaacgta aggaaagata accgtggatc atactacgaa gttccctgct tcgatgaact
    30841 ttttcttctt tcgttaattg aaaagcctca aatatctgca aaatgatctg attcgtttca
    30901 tggattccat ctaataaaac agcttcattg gtatgcggta cactgatcag taactcatac
    30961 actgcttgat ttgaaaaagc aaaggatcga taagtttctg catagatctg cagtgcttcc
    31021 tgaccacttt ttcccacaag agcccgtcgc agctggtcat taagttcctg cagaagtttc
    31081 gctgttagcg ctgttttgac atcacgaata tttttgaaat ggttatacag cgagggagcc
    31141 ttgatatcca agcgtttggc taaatgctga aaggttattt gctgaacatc catctcttct
    31201 gctagttctc gaaagcagtc gatgattttc tcttgagaca gatttctttt tttcatttcc
    31261 atgtttcctc tcataaaata aatccttcac tctttcagtt taccacgaaa aagaaagaga
    31321 aaaaagagac tttcattgat aaactacata ttgtagttta taataaagag ctgtagatga
    31381 aaattttcag aaagaaggag tctaattggc tgcaagaata aaggaaaaat tatttaggaa
    31441 aagagaaact tgggaacaaa acttgtttgt cttatggttt ggaacattta tggctggtat
    31501 cggatttagt ttagtcatgc cttttatgtc actttacata gatacgttag gtaattatag
    31561 tacgtcacaa ttgaactttt ggagcggatt gactttttca tctactttct tggtgactac
    31621 gcttatctct ccttggtggg gaaaattagc cgaccaaaaa ggaagaaaat tgatgctgtt
    31681 gcgtgcttca ttaggaatgg caatcgtcat ttctttgatg ggttgcgtga ctagtgtgta
    31741 tcaattagtc gcattgcgtc tgcttcaagg gattttttct ggttatatca gtaatgccac
    31801 tgcccttgtc gcaactggga cgccaaaaga aaaaagtggt caagttcttg gaaccttagc
    31861 aactggttct gttacaggaa cattacttgg accgcttctt ggcgggatct ctgcttcagc
    31921 gtttggctat cgccctacct ttttcattac agggatcatc ttatttttcg tcttcttgtt
    31981 gagtctgttt ttcgtccatg aaaaatttgt cccagtgaaa aaagaaaaca tggtttctgc
    32041 caaacagatt ttcaaagact tgaaatatcc acacgtcgtc atcggtatgt tcgtcacaac
    32101 gatgatcatc caagcatcca acaattccat tagtccaatc atcagtctgt acattcgcca
    32161 attgctgcat ggacatggga atgttacgtt gatcagcgga atcattgctt cgatacctgg
    32221 gattgccact ttgatagccg ctccaagatt tggtcgctta ggtgacaaga ttgggagtga
    32281 aagaatactt gcgattggtt tgggttttgc gattctggtt tatatcccaa tggcattcgt
    32341 cacgaatgtt tggcaattag ctgccttacg ttttttgatt ggtatctctg atgcttgtct
    32401 gctccctgct gtccaagcat tgatcacaaa atattcgcct catcaagctg ctggacggat
    32461 cttcagttat aaccaatctt tccaagcaac tggtaacgta gtagggccta tgatcggctc
    32521 cagtgtttcc agtgcatttg gttatcgtgg cgtctttctt tcgacttctc ttcttgtttt
    32581 ggtcaacttc ttgcttgtac gacacaatac acaagagatc catcagcaag aaactgcaga
    32641 agaaaaagct tcaggagtct ccatagcttc agggcactct ccttctcata gacaataagc
    32701 gtaaatgaaa aacactgctt gaacagttgt cacagactgt ccttgcagtg tttttgttta
    32761 ggcctgtttt ttcaagaact cagataagag gaataatccc ttttccaagg tttctttttt
    32821 acaacagtaa cctagtcggg catgcttcgg taaatcaaag gcacttcctg gtactaatag
    32881 tactcctgtt tcttctaata aattgataca aaatgtctgg tcatcttctt cgatggctaa
    32941 tttaatgaaa gaagtagaga cgtaattggg agcgacaagt tcaactttcg gttcattggc
    33001 tacccattgt tttaaaatca caaggttctc tgtaatgatc tttttgtttc gcgccaatat
    33061 tttttcttta ttcttcaatg catgtacagc aagatcatct gacaggactc cgccgcaaat
    33121 catcgtgtag tctcgatact ttcgaaaaac ttctgccaac tcaggtccag tagctgtcca
    33181 gccaatacga atacctggaa tggagtaggt tttggatagg gaattggttg ctataccttt
    33241 ttcatagaca tctacgatcg acaagaattc acctttatcc gttaaaggcg catatacttc
    33301 atctatcaat acatatgcat ctactgtacg agcaatttct gcgatttttt ctaaagtttt
    33361 ccgatctagc aatgtgcctg taggattgtt tgcactattc aaacagatga tttttgtttc
    33421 aggtttgact aaggcttcta gctgttctaa atcaaactgc caatcttctt cttccttcaa
    33481 atgaacaaag tcaacagcgg cacctaaaga tttggggatg tcatataatt gctgataagt
    33541 aggaagcatc gaaacaacat ggtctcctgc ttccactaac gcgtatagag ctaaaagatt
    33601 agcaccagtc gctccattcg tttgtaagat gttttccgga tcgacggttt gataaagaga
    33661 agcgacttct tgtttgaaaa catctgatcc ttcgatccag ccatagtcca atggttcgtt
    33721 tttgcgtttt tcgaaaaatt tacttacgct agtcccatct aagccaacga gttcgtctaa
    33781 tgtcaatgct tcgatcgagc tttgtgcaat atcatatacc gctttctttt cccatttatt
    33841 tagccattct tctacaccga aatttgcgat tttcatgttt ctttccccct tatatcttga
    33901 tccagcctac agcaagcaac ccgattgaaa gcagtgcggc cacacaaatc agcccaatca
    33961 ttactttttc ttttcccgta atttgatgtt tggtttcacg acaagcgatc cagtagaagc
    34021 aaaaaccagg aagataggca atactacata aaaggacata agaaaatcct gctaaaatga
    34081 tgcccatcaa ttcaaaaact acagcaatcc caccgatcac tacttgtttt ttgattcctt
    34141 cttttaaaga aaactggact tgatagatcc cgacaagtag ataacaaatc aaaattgccg
    34201 ctgtacataa agaataggca aattgatagg cataatcagt aactaagaaa gtcaataaga
    34261 atagattagt caagattgca gtaacaacta acgaataagt tggggcattt tttttattcg
    34321 tttttcccca acctttcggc aacagaccgt ctcgagccat caacatcgtt gtttctgctg
    34381 ggagcatcgt ccaagacaac caagaaccaa tgatcgaaat aatcaagccg atattgatca
    34441 gcgctgctcc ccaatttccc acgattcctt ttaaaatatt tgccatagca ggttgtgaaa
    34501 tagttgctaa ctcttcttga gataagacgc cgtatggcaa gatcgaagca aggatataga
    34561 taaacaataa acaaagcaga ccaatgatcg atgcttgctg ggcttctgaa cgtttcttcg
    34621 cgcggttggc taaaacactt gcgccttcga tccctacaaa gacccacatc ataaccatca
    34681 aacaaccttt aatctgcaac cagatcgttc cttgctgccc tgttccattc actagattat
    34741 cagatacgtt accccaaaaa tcagcagtga atatattcac tttaaatgcg ataaatacaa
    34801 atattaaaaa taggaataat ggcactaatt tacagatcgt cacgattgta ttgatgacgc
    34861 tcgctccttc aactccattg tttactaata aagtcagacc ccaaataaaa atacttgcaa
    34921 ataaaataga aggaacattt tgtccacctt tgaatatcgg gaaaaattct cccatggcgc
    34981 tcatcagcat cgttgcaaaa gcaacatttc ccagccacgc agacaaccag taaccccaac
    35041 cactgatgaa gccacctaat ttaccaaatg aagcttccgc gtaaccgaaa ataccaccat
    35101 ctagatctgg acgcttttct cctaaatgat tcagcgataa taccaaagct aaaataccaa
    35161 tcccaacgat tatccatgaa aggatcgctg gccctggagc tgcagaacta gccagatcac
    35221 ttgtgatccc aaacacaccg cttccgattg acgaactgac aaccagagca accagtcccc
    35281 atttgctaat acccgtcgat ttcttttcca tttccatcac cctcacttat aaaattgaac
    35341 aaatcacttg caaaagattc ccttctattt tgcaactgct cttgcttaac aaaaagcaac
    35401 tttattcata ttattggata ttatgaactt tttacacaag tatttcaagt ataaaatgaa
    35461 taaaaattaa ttttattgca aaaaaacagc tatgctatcc aaaagaaaag gtgtttcttt
    35521 tgttagccat agctgttttg attcatttaa tatcgcttaa acgtattgta aaacttttgt
    35581 ttcttgatat gcgtggtcga tcaaaccacc acccagacat tccattccat cataaaatac
    35641 aactgcttgt ccaggagtga ttgcacgtac aggttcgtcg aatacgactt ctgcacgatt
    35701 atcatccaat aagcgaactg tcacaggaac gtcttgctga cgataacgga atttcgctgt
    35761 acacttgaat tcttttggca ttggttcatt cgtcgtaaag tggatttcac ttgcatccaa
    35821 gcttgtggca tacaatgcag gatgatggaa cccttgcccc acatataatg tgtttgtcgc
    35881 tagatctttt cctacaacaa accagggttc ttgcgaatct cctccgccac cgatccctag
    35941 tccttgacgt tgaccaatcg tataatacat caagccagca tgttgtcctt tgacttcacc
    36001 atcaagagtg accatatttc ctttttttgc tggcagatag ttgctcaaaa attgtttgaa
    36061 gttcttttca ccaataaagc agattcccgt tgaatctttt ttcttagcag tcgcaaggcc
    36121 tgcgcgttca gcaattgccc gtacttctga tttttccatg ccgcctagag ggaacatcgt
    36181 ttttgaaagc tgttcttgtg ataattggct aaggaagtaa gtttgatctt tattattgtc
    36241 tactccacga agcatgtgga ccacgccgtt ttcatcgcga gtgacttgcg cataatgtcc
    36301 agtagctaca taatcggcgc caagatccat tgcataatcc aagaatgctt tgaatttgat
    36361 ttctttgttg cacatcacgt ctgggttagg tgtacgtcct gcgcggtatt cggctaggaa
    36421 atattcaaat acacgatccc aatattcttt ttcaaaattc acagagtaat aaggaatccc
    36481 gatttgagca gctactttcg ctacatcctt ataatcttcc gtggccgtgc atacaccgtt
    36541 ttcgtccgtg tcatcccaat ttttcatgaa tattccgatc acgtcgtatc cttgttcctt
    36601 taacaaaagt gctgttacag atgagtcgac accgccgctc atccctacga caacgcgtgt
    36661 tttgctgttg tctgtcatga tatcaccatc atttcaatta gattctgaaa catctacgag
    36721 ttgaaggtcg caacttttca gtattttaca ttatacgtca tatttctgaa aatttcatcc
    36781 cttaattatc aagtcgtaaa aatactgttc tactggacct attcagtaca caaacatgat
    36841 agtcaaccaa aaaatcactc atcttttgag cattcgatat aaaaaattgg catactcata
    36901 aaaagggcca gcctttcggc aataaggaat aattttataa attgtgaaaa actatttttc
    36961 aaaattgctt cttatcgctt aaagctgcac gtctcattta caaccttttc tttttagatg
    37021 ttatgagttg ctcagataag tgattttgtt tactctaaga aaaaagggag gcaaaagata
    37081 catgaattac tttatcagtg atatgcactt tttccatgaa cgcttattag gaaaaaacga
    37141 ctttgctccg cggctctttg caaatgtgga tgaaatgaac caacagttga tcactagctg
    37201 gaatgcagta ataaaagaaa ccgaccatgt ttatcatcta ggcgaccttg ctatgcatcc
    37261 gcaatatgaa aaagcaaatg cagaaatttt ggaactggtc actcaactgc aaggaacaat
    37321 ccacttgatc aaagggaacc atgacagtcg cgcttttttc cgttatttag aaaaaaatga
    37381 tcctaggctt actgacggaa gtcccaaatt ttatttttat gatgtaggtg ccatcgttaa
    37441 attcgaccat caacaatatt acttgactca ttatccgatg ttgctcgggc ctaacgacac
    37501 tatccgcaat cttcatggac atatccatca ttacagcgta cctatcgaga acgatgtcaa
    37561 tgtaggcgta gacgcaccgg aaagagaact cttaaaggaa aaactaccat ttggcactcc
    37621 gttatctgag caacaaatca aagaaatcta tgaagcaaag aggaagtacg ataggtcgtg
    37681 aaaaaactgc cctatatcaa gtattaaaca aataaagata attactcata tttttcattt
    37741 gagtaggctt attgccacag gaacattagg atcagaaatg gagttgtaac aagacttctg
    37801 tcacaactcc attccatact attcgtcttt ttgctcatgg tgttctttta ccagtttttt
    37861 ctgtaattct ttatcataat ttcgggcagc tgacgttaaa aataaaacga ttccgcataa
    37921 taaaatcccg attccggaaa acaagaacat tttttctacc cctaaacgat cagctaatgg
    37981 tcctgcaaat aataatccaa ttggacctgt caggttcatc aaagagttga agttccctaa
    38041 gacacgacct aaaacattcg attcataact ctgctggatc attgccatca gcagtgtatt
    38101 gaaatatggc gttgcacaac ctgctcctgc attcaagata agaaagtaca agaacccttg
    38161 actatcgttt ggaaccaatc cgcttgcacc gatcgtcagg cccataatga tgtaggctat
    38221 aataataggt ttcattcggt ctttccatgt tcctataaaa ctgatcagtg caccgcctaa
    38281 gagcattccg actgcgtaga ctacttccac tgctcccgcc tgtccaacgg aaccaccaaa
    38341 gtaatctagc gtcatcaatg gatacagact gattgctggc atgaacaaca gcatcacaaa
    38401 tgcaccattg atcgttatgt accataaccc tttattatcc atcaattgct gcagcccaaa
    38461 ttttgcatct ttaaaaatag taagcggaac ttccaatatt tcaggagaaa cttttgggat
    38521 cttcacaaaa atcaataagc cgacaccaaa aactgccccc aatacatcta ataaaataag
    38581 gtaattgaca ggaacgactg aaaacaaagc cgcacctaga gctggcgcaa tgatgaaatt
    38641 cgctgattgg accattccta actgtccatt cgtccttgtt atgtgagaag atggaacgat
    38701 tgtcggcata atcgactgga tcgttggcat ttggaatgtt tgtgcgaccg aacgcataaa
    38761 caaagacaca aaaactagcc aaatcgggaa ggattctgaa atcgttccga ctactgctaa
    38821 aataagcgcg aaaatcgcta caataatatc cgtgacaatc aaaagagctt ttttatccca
    38881 gcgatccact agcggtccta caaatgggct tagtaaaacc atgggaatca ttcctagtaa
    38941 agtagcaaaa ctcaaaattg tcgccgaacc ggtttctctt gtcaaatacc agatgatcgc
    39001 atattggacg accatactag tgattcctga caaaaactgt cccgataaaa agaacaaata
    39061 atttctcttc caattcgtta aaacgaccga ctgttcattt tccataaaca tccctctttt
    39121 ctttttcatt ttgtatagcg tttgcacaac aatcattata cgggattctt ttttcaaaaa
    39181 ataccttttt ccctaaataa caatcgtttc aatttaaatt tctttactta gaaacaataa
    39241 atcagcaaaa tattaaaaat aacaacatgt gttaaattta aaaataaaaa agacaactaa
    39301 tttgcgtcgt ccttacaaag ttagtcatct aacaataggc cgtttcaaaa ctagttgtct
    39361 ttttatatcc acagatcatt cgactttttc gaataaccca cggctaatca tcccatccat
    39421 cagaaaataa aatttatctt ggctcatttt ctgttttgga tctttgaaaa tattgactgc
    39481 tttcaatcca tttgctgtag tgatcgacat tttcaatgtt tgaatatctt ttgatacagt
    39541 gatttttaat ttgaatcctt ctttgctttg cggcgtggca tctaatggtc ggatcaattg
    39601 ataattgcct ccgtttgttt ctgtaaatcc attatcgcgc aatgtatatc tttttgcatc
    39661 aggatgcaaa cgatagctaa ttggtgcgcc cagtaccgtt gctgttgttt caaatgccat
    39721 gattttcacc tcgataaaat tagtttctct ttattataaa agtttatcct gatttttact
    39781 aggtttttca gttaagttcc ctaaaaaatg aaggcgttct ctcagacatc tgttcatata
    39841 agaatgactt cactagttat tttttgacag cacgaactaa ttgatcaagg aaatactgga
    39901 tgtcgtcttc ggtatttccc aaaccaaagc tgatacggat ggattctttt agtatcggtg
    39961 tatcttctcc atacattgct tccaatacgt gtgaaggctc gatatttcct gctgtacaag
    40021 ctgaaccagt agaaatagcg accccattaa gatcaagctt catcagtagc aaatcatttg
    40081 cttcgccaat taagcgaaga ttcaacacat gagggagttt attcgttaaa tcaccattta
    40141 cttgataagg gatttcgttt gcttctaatg tatcaagaat caattgctgg aatcctttat
    40201 atttgttgtt acgtgcttca cgttcttcag gtgacagcag ttcaactgct tttgccatac
    40261 cccagatccc tgctaaattt tctgtacctg cacgccgctt ttcttcctgc tccccgccta
    40321 aaagcaatgc agggatattt actccgtctc gtttatataa gaaaccgacg ccttttggcc
    40381 cattgatttt atgagcagag atacttaata aatcgattcc taattcttct ggatggatcg
    40441 tttggtttcc gtacgcttgt accgcatctg tatggaaata agctggatga tccgctaaaa
    40501 gttcaccgat ttctttgatt ggcatcaaat ttccgatttc attgttcccg tacatcacag
    40561 agaccaagat cgtatcttct cgtaacgctt ttttgacctc ttcgatcgac agattccctt
    40621 tttcatctac aggcagatat gtaacgtcaa acccctttgt ctctaaatat ttcatggtat
    40681 taagaacagc aggatgttcg atagctgtag tgattagatg tctgccttct ttttgacgcg
    40741 agaacgctgt ttctaagata gctgtattat ccccttctgt tcccccactg ttaaagatga
    40801 tctcgtgagg ttttgcttgc agactgtctg caatgatttg tcttgctgtt tccaattttt
    40861 catgcgcgtg gcgaccaaat ccatgtatgc ttgaaggatt tccaaacgtg tcttgcatga
    40921 ttgccgtcat cgcttcaatt acttgtggat gcatgggggt agttgccgca tgatccagat
    40981 aaatagtttt cacttcattc atcccttcca aattattgct aggcacattc tagcataaaa
    41041 tagcgaacaa accaaagaag agctcatttt tgtctagagg acgtttcatg ggcatagaaa
    41101 aaaggagctg tgacaaaagt cttgtcgcag ctccttgaag ctgctcactt ctgtcacaac
    41161 ttctt
//
LOCUS       DAYYDS010000014        40414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000014 DAYYDS010000000
VERSION     DAYYDS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.014"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACHA02_000781"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACHA02_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8669153.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACHA02_000782"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACHA02_000782"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8669154.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="ACHA02_000783"
     CDS             complement(2303..2965)
                     /locus_tag="ACHA02_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8669155.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="ACHA02_000784"
     CDS             3149..4795
                     /locus_tag="ACHA02_000784"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8669156.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="ACHA02_000785"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="ACHA02_000785"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8669157.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="ACHA02_000786"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="ACHA02_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="ACHA02_000787"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="ACHA02_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="ACHA02_000788"
     CDS             complement(8944..10023)
                     /locus_tag="ACHA02_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8669158.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="ACHA02_000789"
     CDS             complement(10126..10566)
                     /locus_tag="ACHA02_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8669159.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="ACHA02_000790"
     CDS             complement(10623..11033)
                     /locus_tag="ACHA02_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8669160.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="ACHA02_000791"
     CDS             complement(11213..11647)
                     /locus_tag="ACHA02_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8669161.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="ACHA02_000792"
     CDS             complement(11747..12595)
                     /locus_tag="ACHA02_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8669162.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="ACHA02_000793"
     CDS             12984..14750
                     /locus_tag="ACHA02_000793"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8669163.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="ACHA02_000794"
     CDS             14852..16111
                     /locus_tag="ACHA02_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8669164.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="ACHA02_000795"
     CDS             16258..17559
                     /locus_tag="ACHA02_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8669165.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="ACHA02_000796"
     CDS             17562..18413
                     /locus_tag="ACHA02_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8669166.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="ACHA02_000797"
     CDS             18426..19241
                     /locus_tag="ACHA02_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8669167.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19392..20558
                     /locus_tag="ACHA02_000798"
     CDS             19392..20558
                     /locus_tag="ACHA02_000798"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8669168.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20788..21435
                     /locus_tag="ACHA02_000799"
     CDS             20788..21435
                     /locus_tag="ACHA02_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8669169.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21519..22463
                     /locus_tag="ACHA02_000800"
     CDS             21519..22463
                     /locus_tag="ACHA02_000800"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8669170.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22598..24049
                     /locus_tag="ACHA02_000801"
     CDS             22598..24049
                     /locus_tag="ACHA02_000801"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8669171.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24070..24906
                     /locus_tag="ACHA02_000802"
     CDS             24070..24906
                     /locus_tag="ACHA02_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8669172.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24906..25565
                     /locus_tag="ACHA02_000803"
     CDS             24906..25565
                     /locus_tag="ACHA02_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8669173.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            25691..26380
                     /locus_tag="ACHA02_000804"
     CDS             25691..26380
                     /locus_tag="ACHA02_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669174.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26386..27342
                     /locus_tag="ACHA02_000805"
     CDS             26386..27342
                     /locus_tag="ACHA02_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8669175.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27386..27898
                     /locus_tag="ACHA02_000806"
     CDS             27386..27898
                     /locus_tag="ACHA02_000806"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8669176.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28154..28702)
                     /locus_tag="ACHA02_000807"
     CDS             complement(28154..28702)
                     /locus_tag="ACHA02_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8669177.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28791..29972)
                     /locus_tag="ACHA02_000808"
     CDS             complement(28791..29972)
                     /locus_tag="ACHA02_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8669178.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30208..31716)
                     /locus_tag="ACHA02_000809"
     CDS             complement(30208..31716)
                     /locus_tag="ACHA02_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8669179.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31875..32066
                     /locus_tag="ACHA02_000810"
     CDS             31875..32066
                     /locus_tag="ACHA02_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669180.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32269..33186
                     /locus_tag="ACHA02_000811"
     CDS             32269..33186
                     /locus_tag="ACHA02_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8669181.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33179..34252
                     /locus_tag="ACHA02_000812"
     CDS             33179..34252
                     /locus_tag="ACHA02_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669182.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34400..34918
                     /locus_tag="ACHA02_000813"
     CDS             34400..34918
                     /locus_tag="ACHA02_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669183.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34899..35291
                     /locus_tag="ACHA02_000814"
     CDS             34899..35291
                     /locus_tag="ACHA02_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8669184.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35367..36302
                     /locus_tag="ACHA02_000815"
     CDS             35367..36302
                     /locus_tag="ACHA02_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8669185.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            36435..37214
                     /locus_tag="ACHA02_000816"
     CDS             36435..37214
                     /locus_tag="ACHA02_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8669186.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            37207..37524
                     /locus_tag="ACHA02_000817"
     CDS             37207..37524
                     /locus_tag="ACHA02_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8669187.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37768..39378
                     /locus_tag="ACHA02_000818"
     CDS             37768..39378
                     /locus_tag="ACHA02_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8669188.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            39535..40383
                     /locus_tag="ACHA02_000819"
     CDS             39535..40383
                     /locus_tag="ACHA02_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8669189.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13231 a         7260 c         7799 g        12124 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    19321 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    19381 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    19441 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    19501 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    19561 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    19621 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    19681 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    19741 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    19801 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    19861 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    19921 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    19981 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    20041 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    20101 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    20161 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    20221 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    20281 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    20341 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    20401 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    20461 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    20521 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    20581 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    20641 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    20701 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    20761 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    20821 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    20881 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    20941 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    21001 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    21061 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    21121 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    21181 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    21241 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    21301 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    21361 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    21421 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    21481 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    21541 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    21601 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    21661 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    21721 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    21781 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    21841 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    21901 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    21961 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    22021 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    22081 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    22141 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    22201 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    22261 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    22321 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    22381 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    22441 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    22501 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    22561 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    22621 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    22681 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    22741 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    22801 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    22861 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    22921 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    22981 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    23041 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    23101 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    23161 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    23221 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    23281 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    23341 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    23401 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    23461 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    23521 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    23581 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    23641 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    23701 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    23761 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    23821 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    23881 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    23941 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    24001 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    24061 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    24121 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    24181 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    24241 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    24301 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    24361 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    24421 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    24481 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    24541 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    24601 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    24661 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    24721 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    24781 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    24841 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    24901 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    24961 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    25021 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    25081 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    25141 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    25201 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    25261 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    25321 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    25381 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    25441 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    25501 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    25561 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    25621 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    25681 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    25741 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    25801 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    25861 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    25921 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    25981 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    26041 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    26101 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    26161 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    26221 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    26281 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    26341 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    26401 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    26461 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    26521 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    26581 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    26641 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    26701 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    26761 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    26821 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    26881 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    26941 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    27001 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    27061 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    27121 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    27181 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    27241 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    27301 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    27361 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    27421 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    27481 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    27541 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    27601 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    27661 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    27721 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    27781 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    27841 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    27901 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    27961 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    28021 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    28081 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    28141 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    28201 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    28261 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    28321 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    28381 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    28441 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    28501 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    28561 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    28621 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    28681 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    28741 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    28801 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    28861 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    28921 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    28981 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    29041 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    29101 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    29161 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    29221 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    29281 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    29341 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    29401 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    29461 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    29521 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    29581 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    29641 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    29701 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    29761 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    29821 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    29881 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    29941 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    30001 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    30061 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    30121 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    30181 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    30241 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    30301 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    30361 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    30421 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    30481 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    30541 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    30601 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    30661 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    30721 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    30781 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    30841 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    30901 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    30961 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    31021 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    31081 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    31141 atgatacagt tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac
    31201 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    31261 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    31321 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    31381 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    31441 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    31501 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    31561 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa
    31621 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    31681 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    31741 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    31801 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    31861 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    31921 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    31981 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    32041 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    32101 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    32161 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    32221 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    32281 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca
    32341 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    32401 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    32461 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    32521 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    32581 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    32641 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    32701 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    32761 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    32821 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    32881 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    32941 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    33001 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    33061 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    33121 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    33181 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    33241 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    33301 tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac
    33361 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    33421 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    33481 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    33541 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    33601 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    33661 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    33721 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    33781 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    33841 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    33901 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    33961 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    34021 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    34081 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    34141 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    34201 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    34261 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    34321 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    34381 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    34441 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    34501 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    34561 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    34621 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    34681 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    34741 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    34801 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    34861 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    34921 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    34981 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    35041 aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga
    35101 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    35161 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    35221 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    35281 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    35341 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    35401 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    35461 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    35521 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    35581 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    35641 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    35701 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    35761 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    35821 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    35881 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    35941 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    36001 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    36061 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    36121 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    36181 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    36241 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    36301 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    36361 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    36421 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    36481 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    36541 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    36601 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    36661 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    36721 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    36781 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    36841 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    36901 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    36961 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    37021 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    37081 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    37141 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    37201 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    37261 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    37321 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    37381 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    37441 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    37501 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    37561 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    37621 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    37681 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    37741 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    37801 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    37861 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    37921 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    37981 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    38041 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    38101 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    38161 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    38221 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    38281 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    38341 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    38401 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    38461 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    38521 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    38581 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    38641 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    38701 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    38761 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    38821 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    38881 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    38941 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    39001 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    39061 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    39121 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    39181 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    39241 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    39301 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    39361 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    39421 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    39481 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    39541 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    39601 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    39661 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    39721 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    39781 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    39841 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    39901 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    39961 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
    40021 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    40081 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    40141 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    40201 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    40261 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    40321 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    40381 taattccttt tattcaaaaa acataccaaa aata
//
LOCUS       DAYYDS010000015        38315 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000015 DAYYDS010000000
VERSION     DAYYDS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.015"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            271..591
                     /locus_tag="ACHA02_000820"
     CDS             271..591
                     /locus_tag="ACHA02_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8669190.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            745..1650
                     /locus_tag="ACHA02_000821"
     CDS             745..1650
                     /locus_tag="ACHA02_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669191.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1647..2420
                     /locus_tag="ACHA02_000822"
     CDS             1647..2420
                     /locus_tag="ACHA02_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8669192.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2601..4025)
                     /locus_tag="ACHA02_000823"
     CDS             complement(2601..4025)
                     /locus_tag="ACHA02_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8669193.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4236..4682
                     /gene="nrdI"
                     /locus_tag="ACHA02_000824"
     CDS             4236..4682
                     /gene="nrdI"
                     /locus_tag="ACHA02_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8669194.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4747..6729)
                     /locus_tag="ACHA02_000825"
     CDS             complement(4747..6729)
                     /locus_tag="ACHA02_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8669195.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6743..7639)
                     /gene="murQ"
                     /locus_tag="ACHA02_000826"
     CDS             complement(6743..7639)
                     /gene="murQ"
                     /locus_tag="ACHA02_000826"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8669196.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7642..8703)
                     /locus_tag="ACHA02_000827"
     CDS             complement(7642..8703)
                     /locus_tag="ACHA02_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8669197.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8914..9441)
                     /locus_tag="ACHA02_000828"
     CDS             complement(8914..9441)
                     /locus_tag="ACHA02_000828"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8669198.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9508..11010)
                     /gene="eat(A)"
                     /locus_tag="ACHA02_000829"
     CDS             complement(9508..11010)
                     /gene="eat(A)"
                     /locus_tag="ACHA02_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8669199.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11268..12389)
                     /locus_tag="ACHA02_000830"
     CDS             complement(11268..12389)
                     /locus_tag="ACHA02_000830"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8669200.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12515..13456
                     /locus_tag="ACHA02_000831"
     CDS             12515..13456
                     /locus_tag="ACHA02_000831"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8669201.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13606..14502)
                     /locus_tag="ACHA02_000832"
     CDS             complement(13606..14502)
                     /locus_tag="ACHA02_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8669202.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14771..16111
                     /locus_tag="ACHA02_000833"
     CDS             14771..16111
                     /locus_tag="ACHA02_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8669203.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16329..16772
                     /locus_tag="ACHA02_000834"
     CDS             16329..16772
                     /locus_tag="ACHA02_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8669204.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17276..18694)
                     /gene="nhaC"
                     /locus_tag="ACHA02_000835"
     CDS             complement(17276..18694)
                     /gene="nhaC"
                     /locus_tag="ACHA02_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8669205.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19097..20320)
                     /locus_tag="ACHA02_000836"
     CDS             complement(19097..20320)
                     /locus_tag="ACHA02_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8669206.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20351..21190)
                     /locus_tag="ACHA02_000837"
     CDS             complement(20351..21190)
                     /locus_tag="ACHA02_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8669207.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21207..21887)
                     /locus_tag="ACHA02_000838"
     CDS             complement(21207..21887)
                     /locus_tag="ACHA02_000838"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8669208.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21889..22962)
                     /locus_tag="ACHA02_000839"
     CDS             complement(21889..22962)
                     /locus_tag="ACHA02_000839"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8669209.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23018..23256)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23299..23532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23609..24280)
                     /locus_tag="ACHA02_000840"
     CDS             complement(23609..24280)
                     /locus_tag="ACHA02_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8669210.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24367..27015)
                     /gene="mgtA"
                     /locus_tag="ACHA02_000841"
     CDS             complement(24367..27015)
                     /gene="mgtA"
                     /locus_tag="ACHA02_000841"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8669211.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27357..27531)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27704..28141
                     /gene="ndk"
                     /locus_tag="ACHA02_000842"
     CDS             27704..28141
                     /gene="ndk"
                     /locus_tag="ACHA02_000842"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8669212.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28163..29029)
                     /locus_tag="ACHA02_000843"
     CDS             complement(28163..29029)
                     /locus_tag="ACHA02_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8669213.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29189..31282)
                     /locus_tag="ACHA02_000844"
     CDS             complement(29189..31282)
                     /locus_tag="ACHA02_000844"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8669214.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32031..32654)
                     /locus_tag="ACHA02_000845"
     CDS             complement(32031..32654)
                     /locus_tag="ACHA02_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669215.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32813..34210)
                     /locus_tag="ACHA02_000846"
     CDS             complement(32813..34210)
                     /locus_tag="ACHA02_000846"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8669216.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34242..35213)
                     /locus_tag="ACHA02_000847"
     CDS             complement(34242..35213)
                     /locus_tag="ACHA02_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8669217.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35587..36012
                     /locus_tag="ACHA02_000848"
     CDS             35587..36012
                     /locus_tag="ACHA02_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669218.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36500..37201)
                     /locus_tag="ACHA02_000849"
     CDS             complement(36500..37201)
                     /locus_tag="ACHA02_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8669219.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37217..37657)
                     /locus_tag="ACHA02_000850"
     CDS             complement(37217..37657)
                     /locus_tag="ACHA02_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8669220.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37657..38232)
                     /locus_tag="ACHA02_000851"
     CDS             complement(37657..38232)
                     /locus_tag="ACHA02_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8669221.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT        11067 a         8002 c         6676 g        12570 t
ORIGIN      
        1 tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag cattcatctt
       61 cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa gtatgagatt
      121 gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg ccaccgggta
      181 ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct ctttttgtgt
      241 atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat catagcagga
      301 attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc taaaatcaat
      361 tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc aaaggcaatc
      421 aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc agttggttcg
      481 gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg gttctttgtg
      541 gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta gacacttgga
      601 ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt ttatgaaaaa
      661 ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat gcttgtacta
      721 tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt atccaagaac
      781 tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg caaaatcgtt
      841 ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat tttaggactg
      901 atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc ctctagtcat
      961 aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc attcttaaca
     1021 ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat cgacactatc
     1081 gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta ctcgttggga
     1141 atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat cgttatttta
     1201 gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa aatcattacc
     1261 gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag cgagcttgaa
     1321 aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca atgtacaatg
     1381 gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa tgatctagct
     1441 gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa aatcaagctt
     1501 ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc acaaaatatc
     1561 catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt ggaaattctg
     1621 gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa ttatttaaat
     1681 tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg ctcataggaa
     1741 cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg actgctcagc
     1801 tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc acgatcattt
     1861 cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat tctcgaggag
     1921 aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta taccttttag
     1981 ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc ttcacggaga
     2041 aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg ggtagttata
     2101 tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc aatagttccg
     2161 gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata tctggtatcc
     2221 ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt atgttgaatc
     2281 tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt tctactaatc
     2341 aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga tatctcgtat
     2401 ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag acgttcagcc
     2461 ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc tgtgacaaca
     2521 tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc acaacctctc
     2581 tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa aaccattgca
     2641 atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat atgttgtaca
     2701 ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa gaaaagaaca
     2761 agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg aaaggcactt
     2821 gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag tttcatttgg
     2881 tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt gtaagcaaag
     2941 ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca taacgtcgtc
     3001 aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc tttaggcaaa
     3061 agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca tgagagccaa
     3121 acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca tttaccaacc
     3181 atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag ctgattcatc
     3241 gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa gccgaatatc
     3301 cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc tacgacagag
     3361 gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa tacttgactg
     3421 gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa gttatcaatg
     3481 aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa tggcactaat
     3541 ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg attcactagc
     3601 catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt ttgcccacct
     3661 tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa agctacgttg
     3721 cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa acggccaaaa
     3781 ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt attgatatta
     3841 ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact gaaaatagct
     3901 gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc tccaatcgct
     3961 gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg ttgctctgta
     4021 tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac aaaccttttt
     4081 tatttttact taattaataa aaactttaat agaaatataa ttctaccggc ttttttctca
     4141 ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac ttaaaaaaga
     4201 tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat atatcgattt
     4261 caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa atgcatgcag
     4321 aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt gattttacga
     4381 aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga aatggtttag
     4441 ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa catgaagaca
     4501 atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat aatcagtatt
     4561 gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca gattatgaat
     4621 tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa aacaataaat
     4681 aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc tattgtttct
     4741 atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac ctgttttttc
     4801 caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg atgtttcttt
     4861 gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag gtgtcacttg
     4921 ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg tgttgattcc
     4981 catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt gtttcgttgg
     5041 gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt ctggttctac
     5101 aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt tatctaaaga
     5161 aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg aagtttgagg
     5221 tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt cttctttttc
     5281 taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag cataagctgc
     5341 caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga tcaaagcggc
     5401 tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca ctgccccgcc
     5461 gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg ttactccgta
     5521 aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga tcatttctgt
     5581 taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca cttgtcctgc
     5641 accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa tcatttgcac
     5701 atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga ataatgtgcc
     5761 tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc caccgaccat
     5821 gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca gaccgatggc
     5881 aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta ctttatgcag
     5941 ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga tcccaccttg
     6001 tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg gattcatccc
     6061 tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac caaaaacttg
     6121 tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt tcaagatgtt
     6181 catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg tgactaagtt
     6241 tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag gaatcattgg
     6301 cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt tttgtttcga
     6361 tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc cagatgctga
     6421 tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca cgcctgccaa
     6481 atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga cttgaagctg
     6541 ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga catttacaag
     6601 atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc tttctacatt
     6661 gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag tcagttcgtt
     6721 ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt gcttcagcta
     6781 attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata gccagcttaa
     6841 cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct tctgtcgctt
     6901 gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg acatcgacca
     6961 tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc atattcaaga
     7021 taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat tctggacctg
     7081 catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta ttgcaagaaa
     7141 tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc acatatggtg
     7201 tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc agcttgatta
     7261 gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca gtcattgctt
     7321 cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat gtcggtacgc
     7381 attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata taaatcagac
     7441 gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata cttgtcagtt
     7501 ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga acggcttgag
     7561 aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt gttaggttct
     7621 ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat agttcatacc
     7681 agctttgatt tggttgatca atggtagctc ttctttgata actttagcta ctacattcac
     7741 tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat agcgcaaata
     7801 cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc ttgctgggat
     7861 aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg catcgtcttg
     7921 ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag gttccgtatg
     7981 gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt tttctgacag
     8041 acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta gagcgcctgc
     8101 aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata gtgggcctct
     8161 caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt tgagccattg
     8221 attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat aataattatg
     8281 ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga tatctttagg
     8341 cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg caatctgctg
     8401 attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca aaggcgttag
     8461 atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga ccatcaattc
     8521 tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat atgccgaaac
     8581 atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg atgccatttg
     8641 gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg aaaagccaaa
     8701 catattatcc ctctttttct gtatccgtct tcatttctat gactagagta tatatctaaa
     8761 gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa ggttgagttt
     8821 atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa gcagtgcctt
     8881 gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca attctgtatt
     8941 atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag cattgatcac
     9001 tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt cgatgcattc
     9061 tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat ttgggaatcg
     9121 tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca gtccatcata
     9181 atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc tgagacgatc
     9241 agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat
     9301 tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct gtcctttttc
     9361 agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag ttgtttctcc
     9421 gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc ccttaaccat
     9481 accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa tctcagtaga
     9541 tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt tcacgttcaa
     9601 gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg gttcatccca
     9661 tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt tccgttggcc
     9721 catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa gctttcggag
     9781 gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg ccaacgttcc
     9841 tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg gctggtattt
     9901 ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg atgactttcc
     9961 tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa aatgaattgg
    10021 ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa gtcgcttatg
    10081 gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt ttaatagttt
    10141 ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc gtttcatcgt
    10201 ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag tatcgatgaa
    10261 tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg ctttttcagc
    10321 tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag ccatttcaaa
    10381 ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat aaagttccag
    10441 ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc cccggtcatg
    10501 gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt gttttctacc
    10561 agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt catccacaaa
    10621 taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga attctctcca
    10681 taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc atatctcaaa
    10741 atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg tctgttgcgg
    10801 gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt tgttttgtag
    10861 aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta gtttccattg
    10921 tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt cgtagccgaa
    10981 tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa aagaccagtc
    11041 ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa agacccatac
    11101 atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct ccacaaggta
    11161 cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat ccctcacttt
    11221 gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta cttcgggagg
    11281 atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa tgtttgcctg
    11341 tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac aattgggaga
    11401 aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat tattggtttt
    11461 cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat cgtttgctga
    11521 tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt ttccaatcca
    11581 gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa aaaaagggct
    11641 ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac ctcccagaaa
    11701 tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag cgtcgtatct
    11761 attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt atatggattg
    11821 gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct tcgatcaaaa
    11881 tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa agaagcaccc
    11941 aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa agtgacaata
    12001 atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc ataactggaa
    12061 gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa tgcttgtgca
    12121 gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct gatttttcgc
    12181 tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag cgcgtaatag
    12241 attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt tcttccttta
    12301 cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc acttccttta
    12361 aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct taagtaagac
    12421 actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt ctatatacta
    12481 aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag ctggggccgg
    12541 agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg tcctcttgat
    12601 cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg ctgactttaa
    12661 cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca tcgaaagcaa
    12721 cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg atatgttttg
    12781 ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa atggattagg
    12841 tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg ggatcacgat
    12901 gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg gagaaatcga
    12961 actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag aagtctttca
    13021 agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt ggcgaaaagc
    13081 ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata ttgctgaact
    13141 aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat ttgcttctat
    13201 cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata ttgcttctac
    13261 ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc tgatcaaaaa
    13321 ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag gacaaaaata
    13381 tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa aagaaggaat
    13441 ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag ctggttctct
    13501 ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc cataagaatt
    13561 gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt tgttttcaac
    13621 agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga taaatcgttt
    13681 tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct taaatggtgt
    13741 atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa tattttcagg
    13801 acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct tttgaaaata
    13861 catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca gttccagaat
    13921 cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc caggttgttc
    13981 attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta taaattcctg
    14041 cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa atagatccgt
    14101 ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat tatgtttcag
    14161 cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg ccggttcatc
    14221 ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa aaaataacac
    14281 aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa atccataatc
    14341 taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa tgaattccga
    14401 ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca aaaaataaag
    14461 tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg cctcactttc
    14521 tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa aacgtttcct
    14581 atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc attcttctat
    14641 taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg ttatcattag
    14701 tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat ttaaaagaaa
    14761 gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac atatcaccat
    14821 gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg aagcgattgg
    14881 gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt tatatatcgt
    14941 gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg gattatctgg
    15001 tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg tctactggtc
    15061 tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt tgcagttgtg
    15121 gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa ctacgatcat
    15181 caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt ttgctaaaat
    15241 cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc aaatgttggg
    15301 cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta ctcctcatgg
    15361 tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg gttctgaact
    15421 gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta aagcaatcaa
    15481 aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc ttttgattct
    15541 ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg tatttgaaaa
    15601 aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg ctttgttctc
    15661 atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt tgaaggatcg
    15721 aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc aacgtgctgt
    15781 tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg tcggtgacaa
    15841 attatttggc tatctggcag gttccctttc gtatacagta ttattgatct ggatcattat
    15901 cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata aaggaatcag
    15961 cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca cgaactccat
    16021 tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt atcaaaaaaa
    16081 gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat tttatttatg
    16141 tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg caactccttt
    16201 ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc tctgtgcaaa
    16261 ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa aaaaggagtt
    16321 tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca acggtagtta
    16381 tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc agtcgtacac
    16441 gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg ttgttcatgt
    16501 ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt cttcttgttt
    16561 ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca gcttggatga
    16621 aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct gatttaaaag
    16681 atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa gagatcgaca
    16741 aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata aaaaaagagg
    16801 atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga acgccgtgaa
    16861 taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc
    16921 ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt gaacaccgtt
    16981 tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa atggaaaaat
    17041 agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat tgaacaccgt
    17101 ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat tttgtgaaat
    17161 ggctttccac atttttgtat aaatttggct taataccgag aaactggctt ttgaataccg
    17221 tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta tgtttttatt
    17281 gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt tttgagattc
    17341 cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg aaaaatacga
    17401 atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt ccggcaattc
    17461 cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta cctaatacag
    17521 ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc agaataacag
    17581 ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat gttgtaaaaa
    17641 tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt gagatcaagc
    17701 caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac acagtaggca
    17761 tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt ccagtatttg
    17821 aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt gggttttgaa
    17881 taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt gccggcacct
    17941 ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt gaaatagtaa
    18001 agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg gctttacttg
    18061 tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca gttgaccaca
    18121 tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct agatttgttg
    18181 attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata gctccaacaa
    18241 ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc ccaatcgttg
    18301 acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca acaaatacag
    18361 aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga gttggaatga
    18421 tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata aagattggga
    18481 tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc gggtatcttt
    18541 ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata ttaggtgaga
    18601 cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat acaagtgttg
    18661 cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt ttagttgttc
    18721 ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc gcgattcctc
    18781 ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc ggtaccacct
    18841 taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat ggactttcca
    18901 tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct gaacaaaaca
    18961 cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat aagatcattt
    19021 gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata actttcacac
    19081 acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct actatttctt
    19141 gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca agagctgtat
    19201 tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt ttagcagcag
    19261 ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc gtaggctgga
    19321 ttgttggtat gacttggctg acatttccta catcagaaga accgagactt gttctttgtt
    19381 ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag aataagtcat
    19441 caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca aactcaaaag
    19501 tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca tatactttat
    19561 ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa tctggaatga
    19621 cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct tttggcaatt
    19681 gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa gcatttactc
    19741 ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg tcgactggat
    19801 cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg cataacgcta
    19861 catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat ccgttctcgc
    19921 ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca tcaatgaatt
    19981 gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc ccgcatgcat
    20041 gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga ccgggtttat
    20101 tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt tctacccaaa
    20161 acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca tagttactta
    20221 cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa cgatattgga
    20281 ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc tcctatgcta
    20341 gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat ccttttgatg
    20401 ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt ttttgatacg
    20461 ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga cgtgcatggt
    20521 catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca tctacagcta
    20581 caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt tgggaagcat
    20641 ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca gtgggcgctt
    20701 gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga agagctctgc
    20761 cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata tcagctacat
    20821 ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg atcgatacta
    20881 aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg aaagaattca
    20941 aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta aatttcacgt
    21001 attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat acatctgtat
    21061 cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta tctgcttttc
    21121 cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca aaacgaataa
    21181 tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag ttgattactg
    21241 ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat cgttacatct
    21301 gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc tgcacctact
    21361 gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga gacccgaatg
    21421 ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg actggttccc
    21481 atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt ctcgatttga
    21541 cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt cgcaccaatc
    21601 gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat aatgaaaggg
    21661 atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg gttagctagt
    21721 aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat catcccgcca
    21781 atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac aaattcatcc
    21841 ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt atcttcctcc
    21901 ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta tttttacacc
    21961 agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag ctaaagtaac
    22021 gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata aaatattcgc
    22081 tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct gatttccaat
    22141 ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag aatgggcaat
    22201 gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt tggtcaattc
    22261 ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc ttccatcttc
    22321 cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag tgatcaggac
    22381 gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct gaagggtcgt
    22441 tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt cattcgccaa
    22501 agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag gataagcaga
    22561 tgctttttct tccaaaccga caagttccag tagttcattc actttttgac gacgttcctg
    22621 tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag ttcggctttc
    22681 cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc gcaattcttt
    22741 gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg ttggtttctg
    22801 cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac cgacgattcc
    22861 ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta tctctttttc
    22921 tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc ctccttatct
    22981 aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt ttttgcgtgt
    23041 taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg atcatactct
    23101 agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg aattgctcga
    23161 tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc tctgtcaaag
    23221 gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa gtccttttac
    23281 taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt ttcggaagtt
    23341 tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta acggtcgaac
    23401 agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc agcttttctt
    23461 acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta ctttcctttt
    23521 caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg aaaagtcaag
    23581 tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt aacatttcaa
    23641 taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt tcttcgacgg
    23701 tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct gctcgtgaag
    23761 cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct tctaatcctg
    23821 ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac acttcgtctc
    23881 cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata tctgttgctc
    23941 ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt aagtctgcca
    24001 atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt tgataaaaat
    24061 attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct gctttcacta
    24121 aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt tcttttggga
    24181 tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt ttttctgtgt
    24241 caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta atcctttcca
    24301 aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa gaaccagcct
    24361 tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag ctgtcacaag
    24421 cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag gaagtggcat
    24481 caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa taattcctaa
    24541 tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta ctttcggtgt
    24601 acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc atcctgcatg
    24661 gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac ttccgcccac
    24721 tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa atatcgaact
    24781 tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt tttttggctc
    24841 gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa catcatagat
    24901 cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga acgtacttgc
    24961 aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga tatatttcat
    25021 gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg tcaaatcttt
    25081 ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat ctacagaaat
    25141 accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta aaaatccgac
    25201 agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag gattcaattt
    25261 tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca tttccgagat
    25321 tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca ctgaacgtgt
    25381 aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt tcagagcttt
    25441 caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac caatcaaaac
    25501 catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg tcttttgtgc
    25561 gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa gtacggcttc
    25621 ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt tagaaatgtt
    25681 cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg gatcagcaac
    25741 cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt tttgataatt
    25801 gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat ctaacaaatt
    25861 tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac gatcatcttc
    25921 atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca atgtccccgt
    25981 tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca aatttttgat
    26041 cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg taacgatcat
    26101 tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa aagcttctag
    26161 ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca atgacataaa
    26221 tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt gaattggttt
    26281 ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag aaacaatgat
    26341 tccttccgca cttccactga tgacattact tcccataaag acaagatttt cgtaacttgt
    26401 ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat agctttcccc
    26461 tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca tatccgctgg
    26521 tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt ccataggaat
    26581 ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg atttcagttt
    26641 ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc tgacgataac
    26701 catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg ctaatacata
    26761 ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg gcgtgatgta
    26821 tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg cattttcacc
    26881 gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg atgcatggaa
    26941 tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt tttgtgtatc
    27001 tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt ctcgctgctt
    27061 ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct gagaacagat
    27121 agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt catccatttg
    27181 ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt tcgaatagtt
    27241 atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg cggcaacaaa
    27301 aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa aaagcttctc
    27361 agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct gcttttagct
    27421 accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc tctcgacgtt
    27481 tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat tcggttttcc
    27541 ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt ttttcatact
    27601 ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc tcattttcca
    27661 cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa gaacacttgt
    27721 tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc aacgttttga
    27781 agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg aattagcaaa
    27841 agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg attacatgac
    27901 ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa
    27961 gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag gagactacgc
    28021 gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg cggtaaaaga
    28081 gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag cggcgaagtg
    28141 atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc agttttttct
    28201 ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag aattcttggt
    28261 agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa taatctgcca
    28321 tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt ttcatatctt
    28381 cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa gtcaggttca
    28441 ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt cgcattcttt
    28501 ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa ggaaaatcag
    28561 ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat tcttcaatga
    28621 ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt ttttcgacca
    28681 tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc ctacggttat
    28741 tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc attcgtcttt
    28801 ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat tctgcaagtg
    28861 tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt ttcactgaat
    28921 ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat tgggactgtg
    28981 tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata atagttctcc
    29041 tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga aaaatacacg
    29101 aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat tagaatgttg
    29161 tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt tcaacgtctg
    29221 ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa aagacacttg
    29281 tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca tttttatagt
    29341 catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc aagagatttt
    29401 cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga tctttggaga
    29461 tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga ttattgtaag
    29521 cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga aaagcaacta
    29581 cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct ttatcatcga
    29641 ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat tcatgattga
    29701 ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc atctgtgcgt
    29761 tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa tctttattat
    29821 ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt tggtaaatac
    29881 cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa tgtgccgttc
    29941 gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg aaattcttgt
    30001 catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa aacggctgtt
    30061 gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat atcccataac
    30121 ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca taacctaagt
    30181 ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa acagcgcttg
    30241 tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg tctgacccaa
    30301 gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac atattttcag
    30361 catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg atcgttgctt
    30421 gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc ttcatattaa
    30481 tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct ttagcgatcc
    30541 caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa acttcatcaa
    30601 tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg attccgtcat
    30661 aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca aatgttcgct
    30721 gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa ataagccctg
    30781 aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg ataggtattt
    30841 tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc cagtagatga
    30901 catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac actttcgaaa
    30961 agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa ataatattca
    31021 agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac gctggctttg
    31081 gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt gggttgatcc
    31141 acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca tagtaggcaa
    31201 tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa cgattttggc
    31261 acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt ttcttatttt
    31321 gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt ttattctttc
    31381 taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag cgattaaact
    31441 aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa aagccagttt
    31501 ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct ccttatttct
    31561 tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg ccaactcctc
    31621 ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt gttccttgtg
    31681 gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgaccc
    31741 tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg gtgttcaaaa
    31801 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta ttttttgaaa
    31861 ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca
    31921 atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact attttgccgt
    31981 ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt tcattgtcca
    32041 taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg attttcttca
    32101 cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt cgactcgttt
    32161 ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca ttccgctaaa
    32221 cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac ttttccattt
    32281 ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa atgcttgtct
    32341 ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt aataaattga
    32401 ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta attggcagtt
    32461 attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc tactgattct
    32521 ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa
    32581 aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag aagaaacggt
    32641 ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta ttatatacaa
    32701 aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa cttctttttc
    32761 acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt tcttaagcct
    32821 caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg attgttcttt
    32881 ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat gcattggtcc
    32941 acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca ccttcttgaa
    33001 tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa tcatcgtgga
    33061 tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg ttttcagaaa
    33121 taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca tagattcctt
    33181 gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt tcataattgt
    33241 cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct tttgctttta
    33301 cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct ttgataaccg
    33361 tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc tcaatcaatt
    33421 cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag aacgcatctg
    33481 ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag cttggtgtca
    33541 gattcaagat gataccaata acaccacctt gctccatttc atggtaagat ttgatggcta
    33601 acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata tctgactctt
    33661 ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt tcattatgag
    33721 taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca tatttttcat
    33781 aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac atcggcatat
    33841 cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca tcaaaataac
    33901 gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt atcaaacggc
    33961 tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac tgtacatcct
    34021 cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca aagaactttt
    34081 gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg cctgaaatcc
    34141 ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct ggaaattgat
    34201 attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat tccatgtatg
    34261 atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat tcgttttaac
    34321 agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt gattttcgga
    34381 aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat atccttcccg
    34441 attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt gtaattgctg
    34501 acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata gccgcttttt
    34561 ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg gaccgcttct
    34621 ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc aataaatccg
    34681 atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact ttctcgatca
    34741 ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc gataactgga
    34801 atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa gaagacaatc
    34861 agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt ttctggctga
    34921 tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct ttcgatccct
    34981 gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc aatagagaag
    35041 cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa cgctgaaatc
    35101 gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa gtaacgggaa
    35161 actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc catacattct
    35221 cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat gaccgcattc
    35281 tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt agagcaactt
    35341 cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt aatatagcat
    35401 ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg attttttgcc
    35461 tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg tttttctctt
    35521 ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc aaatggggga
    35581 agtattttga agaaattaat cgtactattt attttgtctg cgggactttt gacattgact
    35641 ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga ttcttctgtc
    35701 ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt acgtaaagat
    35761 atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg gacacgcacg
    35821 tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt gctgacagat
    35881 atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa taaaaaagaa
    35941 acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga tggcaacatc
    36001 tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt ttctgctata
    36061 gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc ctgattattg
    36121 tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa gcttgagcat
    36181 atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat cttatcctgt
    36241 tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc actatggctg
    36301 atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag taaaacaaac
    36361 agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca aatatttgaa
    36421 tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt tttctttccc
    36481 gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg ttatccgtaa
    36541 aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc tgacggatca
    36601 ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc acttgtattt
    36661 taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac gtttcataat
    36721 aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt tttttgatac
    36781 gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc cgtttttttg
    36841 gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac tgctgtactt
    36901 cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta tcttcataga
    36961 tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct aaaaatcgct
    37021 tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa taaaagagcg
    37081 gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc tcttttgcaa
    37141 gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa atcactgaca
    37201 tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc aatgtttcaa
    37261 aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt tgtgtaatca
    37321 tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc aacatagttg
    37381 ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag tcaaggatag
    37441 caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact tcctgcgccc
    37501 atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag tagaaacttg
    37561 tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg atttgctcct
    37621 ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct tcaataataa
    37681 ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat tgttggaaaa
    37741 gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag tttctaataa
    37801 ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct tgtcgggcgt
    37861 cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac ctagacctcc
    37921 agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt catcgatcgt
    37981 ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa aaagcccatt
    38041 cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa tcactcccat
    38101 aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca tgtattttcc
    38161 taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac cgcaaaaaac
    38221 agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc tacacgtgtt
    38281 tttttaaaat aaaagactag attttgtagt ttttt
//
LOCUS       DAYYDS010000016        38015 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000016 DAYYDS010000000
VERSION     DAYYDS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.016"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(424..1368)
                     /locus_tag="ACHA02_000852"
     CDS             complement(424..1368)
                     /locus_tag="ACHA02_000852"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8669222.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1664..2608)
                     /locus_tag="ACHA02_000853"
     CDS             complement(1664..2608)
                     /locus_tag="ACHA02_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8669223.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2829..4043
                     /locus_tag="ACHA02_000854"
     CDS             2829..4043
                     /locus_tag="ACHA02_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8669224.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4065..6080
                     /locus_tag="ACHA02_000855"
     CDS             4065..6080
                     /locus_tag="ACHA02_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8669225.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6163..7536)
                     /locus_tag="ACHA02_000856"
     CDS             complement(6163..7536)
                     /locus_tag="ACHA02_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8669226.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7562..7846)
                     /locus_tag="ACHA02_000857"
     CDS             complement(7562..7846)
                     /locus_tag="ACHA02_000857"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8669227.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7848..9863)
                     /locus_tag="ACHA02_000858"
     CDS             complement(7848..9863)
                     /locus_tag="ACHA02_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8669228.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10042..10344)
                     /locus_tag="ACHA02_000859"
     CDS             complement(10042..10344)
                     /locus_tag="ACHA02_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8669229.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEVALAGTNSGETEDKEESLAGTN
                     SGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10433..11464)
                     /locus_tag="ACHA02_000860"
     CDS             complement(10433..11464)
                     /locus_tag="ACHA02_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8669230.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11671..12546
                     /locus_tag="ACHA02_000861"
     CDS             11671..12546
                     /locus_tag="ACHA02_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8669231.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12698..13540)
                     /locus_tag="ACHA02_000862"
     CDS             complement(12698..13540)
                     /locus_tag="ACHA02_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8669232.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13632..15509)
                     /locus_tag="ACHA02_000863"
     CDS             complement(13632..15509)
                     /locus_tag="ACHA02_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8669233.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15506..16927)
                     /locus_tag="ACHA02_000864"
     CDS             complement(15506..16927)
                     /locus_tag="ACHA02_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8669234.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16930..20319)
                     /locus_tag="ACHA02_000865"
     CDS             complement(16930..20319)
                     /locus_tag="ACHA02_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8669235.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20576..21967
                     /locus_tag="ACHA02_000866"
     CDS             20576..21967
                     /locus_tag="ACHA02_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8669236.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22040..22687)
                     /locus_tag="ACHA02_000867"
     CDS             complement(22040..22687)
                     /locus_tag="ACHA02_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8669237.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22695..23381)
                     /locus_tag="ACHA02_000868"
     CDS             complement(22695..23381)
                     /locus_tag="ACHA02_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669238.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23387..24460)
                     /locus_tag="ACHA02_000869"
     CDS             complement(23387..24460)
                     /locus_tag="ACHA02_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8669239.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24682..25356
                     /locus_tag="ACHA02_000870"
     CDS             24682..25356
                     /locus_tag="ACHA02_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8669240.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25361..26701
                     /locus_tag="ACHA02_000871"
     CDS             25361..26701
                     /locus_tag="ACHA02_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8669241.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26850..27836)
                     /locus_tag="ACHA02_000872"
     CDS             complement(26850..27836)
                     /locus_tag="ACHA02_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8669242.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28560..29591
                     /locus_tag="ACHA02_000873"
     CDS             28560..29591
                     /locus_tag="ACHA02_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8669243.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29700..31232
                     /locus_tag="ACHA02_000874"
     CDS             29700..31232
                     /locus_tag="ACHA02_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8669244.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            31371..32183
                     /locus_tag="ACHA02_000875"
     CDS             31371..32183
                     /locus_tag="ACHA02_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8669245.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            32173..32658
                     /locus_tag="ACHA02_000876"
     CDS             32173..32658
                     /locus_tag="ACHA02_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8669246.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32681..33226
                     /locus_tag="ACHA02_000877"
     CDS             32681..33226
                     /locus_tag="ACHA02_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8669247.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33299..34942)
                     /locus_tag="ACHA02_000878"
     CDS             complement(33299..34942)
                     /locus_tag="ACHA02_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8669248.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(35071..37299)
                     /locus_tag="ACHA02_000879"
     CDS             complement(35071..37299)
                     /locus_tag="ACHA02_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8669249.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(37332..37811)
                     /locus_tag="ACHA02_000880"
     CDS             complement(37332..37811)
                     /locus_tag="ACHA02_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8669250.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11090 a         7195 c         6913 g        12817 t
ORIGIN      
        1 aaaaatgata aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa
       61 tctgcttatg caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa
      121 ttgaatgata aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga
      181 atcattgtat ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac
      241 gattcgctta ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt
      301 cggaccgatt aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa
      361 ctgcttaaca gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt
      421 ttcttataat acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc
      481 tttttcttct tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat
      541 tgatggcaca cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac
      601 aggcaataca gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc
      661 aataccatag taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt
      721 atcagcaatc acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa
      781 cactggttta ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc
      841 acctaagata tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt
      901 acggaaacga gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc
      961 agattcgttc caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat
     1021 accgtcgaat cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt
     1081 tttaactaaa tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac
     1141 gatatcagca tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc
     1201 ccaagccata ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc
     1261 gatcaatact aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc
     1321 aacaaaacct gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct
     1381 ccttttcata actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca
     1441 acatgttaat tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc
     1501 ataacagata aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta
     1561 gaactgaaaa taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc
     1621 taaaacaatt ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc
     1681 attcattggg agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt
     1741 tatatccgat acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca
     1801 acgcaaagtc gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt
     1861 tcaatttatt actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg
     1921 tacaagaagt aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc
     1981 atccatctgc tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata
     2041 gtgatgtata gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca
     2101 gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt
     2161 gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg
     2221 tgcttgccaa atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga
     2281 ttaaaatatc ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca
     2341 ccacgtcact ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc
     2401 tatacatata attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac
     2461 ggtgtttact gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg
     2521 gtgtctgaag atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca
     2581 gagtttcgat tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga
     2641 ctccctcctt tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata
     2701 tgcaaagcta tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga
     2761 ttccgctatt gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg
     2821 aaaggaacat gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta
     2881 ttttgacttc tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca
     2941 cgcgttcttt atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt
     3001 ttgttttgat tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg
     3061 gattagtgat tgcatcgatt tgtggtatcc tccccctctt ctcacaaagt tacagtattg
     3121 tcttagcttc aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca
     3181 gcatgattgc tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg
     3241 catttcaagg atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg
     3301 gttggcaata taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt
     3361 tgtttgttcc tacacctgaa acaactgaac ttcaatcgca tggcacagcc ccaacaagac
     3421 aagtaatcaa aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg
     3481 tggtatataa tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa
     3541 acgaaactaa tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag
     3601 tttgttttgg ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac
     3661 tgatgggaat cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg
     3721 gtgcgatcct aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa
     3781 gtatccaagc gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta
     3841 atttaggtac tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga
     3901 gtaaaacact tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta
     3961 gccttctagt agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa
     4021 aagaacaatt gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac
     4081 ataaagatag atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa
     4141 atttccaacg ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc
     4201 atcggaacgt caagtttaag agatattact ttcttgatca accttccatg tttaaagtga
     4261 cgatccaagg agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc
     4321 tgacttgtcc agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac
     4381 cagcgatgtt tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt
     4441 ttattgaaga atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa
     4501 atcaaatcta ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag
     4561 gaatcttatt cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg
     4621 accatatcga atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg
     4681 attattgcta ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt
     4741 ttcgctattt gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg
     4801 aatttttatc tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga
     4861 tctggtcagt atcagaagaa gccttcaagt tatgtagtga tttgtttttt atcgacggta
     4921 tcaaacgaga tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat
     4981 atcttttttt tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg
     5041 aaatcaagca gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg
     5101 aaaaccacta tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc
     5161 aatcattaat gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag
     5221 caaaagattg gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag
     5281 aacaagtctt attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg
     5341 aaacatatgg agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat
     5401 tgaaacaaaa cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct
     5461 atgaatctgg gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt
     5521 ttgctaacaa agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca
     5581 cacagattac tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac
     5641 agacgaatat cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg
     5701 cggtaacttt ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt
     5761 atggagatcc ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt
     5821 taggacgcta ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac
     5881 aacctaatct agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga
     5941 tttcgcttga aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag
     6001 tgaaaatgaa tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct
     6061 attcaaaaat aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc
     6121 ttcttctgat attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga
     6181 aacaatgttt gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa
     6241 agcgatgatt ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag
     6301 tcctggtact agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa
     6361 tactggaagg ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa
     6421 taccgcccct cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa
     6481 gaaatgtcct actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat
     6541 ggatagtgtt cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata
     6601 tggaaaaacg ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat
     6661 cccttggaat gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac
     6721 aaacccagct gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt
     6781 gcttagttct ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat
     6841 cgacatcaag agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat
     6901 ctttatgtcc tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga
     6961 agcataaccg atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt
     7021 cataaacggt tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc
     7081 taataagatc atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag
     7141 acaagaaaca taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct
     7201 agcaagtaaa atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta
     7261 ttgttgcaaa gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag
     7321 agctgtttgg aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc
     7381 gatcagtact aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt
     7441 cttcttctga agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact
     7501 gacgattgac acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt
     7561 tttattcttt tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg
     7621 attgcaaagg tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct
     7681 caatgaagaa ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt
     7741 caaactcgtc cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca
     7801 taaaacttgt tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct
     7861 tttcttatga tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt
     7921 tgtttattag aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg
     7981 atagcagcta aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca
     8041 ggttgttgaa gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca
     8101 tgaggtaatg cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg
     8161 atcatagctt tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca
     8221 aatgtgattg cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt
     8281 aataattcag atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta
     8341 cgatttaata cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc
     8401 agtagcgccg acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct
     8461 ctttgtactt gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca
     8521 tagagagact ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa
     8581 gaagtttcgt caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc
     8641 gggaataatt gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag
     8701 acgatcacgg ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg
     8761 ccaaatagta tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt
     8821 tctaacggaa gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt
     8881 tgatcgatca tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc
     8941 aagtgataaa agacattcat caataattca cgaaacgaag caaattctac ggccatcagg
     9001 aattccatgc gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc
     9061 tggacatctc cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt
     9121 tgatgttcgg aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga
     9181 gaagccaaca gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt
     9241 gtcaaactca aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat
     9301 agtgaaatcg ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt
     9361 gcggttaaat acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga
     9421 ttttttgctt ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta
     9481 gtatacgaca gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac
     9541 aggactgtcc ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt
     9601 gcagtcgaat aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct
     9661 tcaaatcgag taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata
     9721 cggatacggg tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt
     9781 gtatttactt cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg
     9841 agataactaa ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta
     9901 gcgcttttaa atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta
     9961 ccgaacgatt tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt
    10021 ttatgaattg atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc
    10081 aaatttttct ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc
    10141 attaggcttt gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc
    10201 tgtttcacca gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt
    10261 agttcctgct aaagccactt cttctgtttt aggggccatt gaacctctta atcgtttgtg
    10321 tttttcaccg tcataatttg tcatgttggt tcctcctagt agttctatac tttttcatcg
    10381 tacaataaga cagtttatgc gtcaaaaaac aaacccgatt cttacaaata tgtcatattt
    10441 ttccttctga ttcttgaatg cttttgattc cttcttccaa aattttcatt tgtaagattg
    10501 tttcttcatc tttgatgact ttttctgcgc cattaactaa tgtctcgtaa atcccttcgt
    10561 agacacggct gtaatcacca acctcggaaa ttactttttc ttcatgataa ctcccttgtt
    10621 catctatata ggtcaataca ccataatgtt cgggcaaatc gataccaaag tcttcatgat
    10681 caggcatgta aaacattttc agatgttctt cttgtcgatc ttttgtttgt ttcacgaaca
    10741 tccctttttt tccgtaaacg acgaaacttg gacgttcttt gatgcggaaa taactggatt
    10801 ttatcgaaat tttcatattt ccatagaaaa aatccagatc gaagtaatca ttcattcgtc
    10861 ctttccctaa aagttgacgt acatcatagc tgatatcatc tggttcgccg aaataagaaa
    10921 gaacttgatc tactgtatga caagcatgtc catataaata gctgttcgca gtagaaaatt
    10981 catgcgtatt ttctggtact tccggtctga aataatcata atgcatttct acttctaaaa
    11041 tatcccctaa ttttccgctt tcgattactt tttggactgt caagaagtca gaatcgaacc
    11101 gtctgttttg gtaacattgg ataaatagac ctttttcttt tgctaatgca aataactctt
    11161 ctgcttcctt tgacgtttct gcaaaaggtt tctctactag tacattctta ccatgttcga
    11221 gtgccatttt tccatattca taatgaaatt gactaggtgt agtgatgaca actaattgga
    11281 tctcaggatc atcccaaaga tcagacagct gatcagtata ttcgatgtct tcgaatgttt
    11341 tccacactgg ttcagatggg gaaaaaattt ttttgatatt gattttgttc gtaagttttc
    11401 ttgaaaaagg caaatgatag cgatttgtac tctttccatt gccaagataa gcgattgtaa
    11461 gcatgagtgg tgcctccttt tattgatgtt ttcagtatat cctagaaaag cgcagaaagt
    11521 atttataata gctattctgg taaaaaagtt cagaaaaaca cttgttatca ttcggtttgg
    11581 tgaaattaat gttcaaagta aggataaaaa tcaaaaagat agtagcggaa ccaagtgttt
    11641 atcagtaaga tagaatttgg aggtcagatc atgcttattg aagaaaaact aacaaaacaa
    11701 gagttgttta ccacgacaga gaaacggatt gtcgattaca tccgtaggaa tatcgaagct
    11761 gcagtctaca tgacgattga agaattagca aaagctacgt atacttccca ttcagctatc
    11821 attcgtttat gcaaaaaaat gggattcagc ggatttaaag aattccgttt cgagctgggg
    11881 cgggaagttc atcaattgat tgctcagttc aatcaaacga ttgatcctaa ttttcctttt
    11941 tcaagtgaag atagcactca gcaaattgca aagaaaatgg cggatttatc gatccagtca
    12001 ataaaaaaag cacaattgca gattgaacat gaagatttag atcaaatcgg taaagtattg
    12061 aacaaggcac agcgaatctt tctttttgca aaaggtgatt ctcaaatcac ggcaaggaaa
    12121 ttccagaata aaatggtcaa gttgaataag tttttgatca tggctgaaga atacagtgat
    12181 tcttcttgga atgcagccaa tttgactagc gaggattgcg ccatttttat tagctacagc
    12241 ggtcgcatcc atcattatga acgaataatg acctatttga agcatgtaga ggcccctacc
    12301 ttgctgatta ccggaaatca gcattcagaa atggccaaac aggctgatat gtgtcttgtt
    12361 atatcgcagg aggaatatga ttttgctaaa gtagcgactt tctcctctca aattgccttc
    12421 gattacgtat taaatacgtt attctcagtg atatattctc aaaattttga agagaatatc
    12481 cgcaacttga aaaacaaaca agaattgatg caaaaaggcc ttcttaaaga atatcggaaa
    12541 acttaagaaa agatcctcat ttagtaataa gaaagatagt gtttgatagg ctctctttga
    12601 tatcatcata aaaacaggct gaagttttct ttttcaaacc tcagcctgtt tgtttacgga
    12661 acgattcgaa tagatatatt atagaaatga ggaatagcta acttctgttt cttattttga
    12721 acgattgacg aaaaagatat ataagaagga ggaaaatcag tatacatgca gctaacaatc
    12781 catacaggaa aaatcgtttg ttctcggata ctttctgtac ttctttttga acttcttttg
    12841 catgataagg aacccggtgc cctctgacaa gtaagcgatg actgttgacc atataaggtg
    12901 tacaagttag gagtgtcacg aaatcctgcc ctttttcgat tctcagatct tttgtatcag
    12961 tcggttctac cgtcttgatc tgatcgactt gataagcgag tgtccgtcca ttgatttcga
    13021 tatagaaaga atcacctaaa gtcagtttcg gtaagtctgt gaacaacttt gcttgaggca
    13081 gtcctcgatg gctggaaata acggcatgtg tattttctcc ccccgtagga taagacgttc
    13141 cttccaataa tgaggcacct ttttctagca gcacagtatt cgtttcatca aaaattggca
    13201 aatggacatt gatctcaggg atcgtcaaaa taccgatcgt atgtttctca aaataggttc
    13261 tatccttttt cgcaggttct tttttcttag taaagggatc tgcacctgga tttccacctt
    13321 cttttgctaa ttgctggttt ttctttgtca tgttttcttg gattttagcc atgacttctt
    13381 cattttcttt gaccgcttgt tgctgataat gagagatgat ctgctgatcc agataattat
    13441 tcagcgcgtc gctaacaaat ggataagcta gtatccctat tcctgagaga agcaagaaaa
    13501 gcatcagcag atctagaagt ttccttttct ttggtttcat ttgtgttctc cttgtgatga
    13561 ctgtttgata ataaaagaaa aaggagcatt tgcgtaatcc gctgctcctt tttccaattc
    13621 ttgcaaaaag actaaatctg actgtgcttg cgtctagcaa agtacagtcc agcaatcagt
    13681 agaaggacta ctcctgcacc gatatacaca tagattccct taccgcctgt tgaaggaagt
    13741 gtacctttgt gtttatttgg tattttttca ggagaaatca actgtcctgc tgttacataa
    13801 gattgttcat cgatagtaaa tgctacacgg tttgttaatg gaacatattt ttctggcgct
    13861 ttcgtttctt ccagatagta cgtgccatat tttagacctg tcacatcgat taaaccgtta
    13921 cttgtggttg taaaaaccgt tgctgattct ttcgccgata cccagctgac ggcttttgtg
    13981 gatgggtcga tcgataaata tttcgctgtg tcactatctt gatcacgaac gacgaattct
    14041 gctccagcaa gtgtttggtc tgatgtaacg tcaccatcta ctttaacaaa tcgtttgccc
    14101 ccagtaacga catcgactga cggtggtgtt tgatcatttg tatgcccgtt atcgacattt
    14161 gcttggttag aaaatccttt ggttggatct gctttttcgt tcaaatgcat atagtaaacg
    14221 aatttcaatg taccgccagg agttaatgaa ggaatataat tcggatcaac tgaaaccgtg
    14281 aatccgtctg tttgctcagt gacagaataa tttactgggt cgatttcttt atttccatca
    14341 tataaggcat aagcatacgt tcctgaagaa tcattatcaa atgttaaagc agcgtcatga
    14401 gtatcgataa gtttgaatgt tgtgtacttg ttttgcgttc cttctttatc agcgatacct
    14461 aatgggatat tgacagaaat ctcatattga attttttcac cgattgcgac atcttttcca
    14521 ttcaattgag gtgttgtttt atcaacttta gacgtatcat ttttgatggt cttttctact
    14581 ggtgtttggc tatttgccag gatctcaaaa ggcgtgattg tttgcttttc aatcatttcc
    14641 gcattatcag gagcttttac ttcttctaaa ttatattttc caacttctaa atgattaacg
    14701 atcaactggc ctttttcaat agatgcctcg gcaaagtcat tgttgccgat gtcataagaa
    14761 tgaccagtaa tgaaatgttt ggctttggtt tgatcagttg tccaagtgta caatccatct
    14821 gtgacacttt ggatgtattt tttgacgctt ggtgttcctt cttctttaga aataataaat
    14881 tctgctccat ttagtgcttc gttttcggca gtaccgattt ttgtaacttt caacgttcca
    14941 tcattaccga ctgtattttt agggtagaga tggatagtat ctagttcttc tgtcccgtat
    15001 ttataagagc catctgcttg tttgatcatc tcatatacag ggaaagctaa aaccatgttt
    15061 gcggcagctg acactccgtc ttttggttct tctttgatcg tgtagactgc atctttccca
    15121 ttttgttttt taggtaaaga taaagtgaca tttccatcag catctgtcgt tcctgaagca
    15181 actggtgtac caggagtcaa agactggact gcttgttttg ctgcatccac ggacgctcct
    15241 ttatctcgtt gcgcataaaa ttcttgagtg acgttataaa ctgaaaatga aatatcggct
    15301 aatccttggt attgatcgaa ttcgctcatt tctttcccgc tgttttggat taaaggatcg
    15361 ggtaaatcag tcattttctt tttatgaagg atgacttgag ctgctgtctc ctctgcaaac
    15421 actttttttg cgccgaagct gtttgtgagt aatggtaaaa taaggaaaag gactcctaac
    15481 ataacgttta tttttttatg atttttcatt ttttcactcc tgttcttttt ttgataaaca
    15541 accagattgc aaagcttgcg aataccatac caacaacgcc taaaagtgta tttttcgttt
    15601 cgtttgtttt tggaagaaat ggacggctat tttctttttt attgactact ttcaatatac
    15661 tgtccgcctc tttatcctca gaaagaaaag ttgaaatggt aaacggaatt tctgtttcac
    15721 ttaaaatata accagaaggt gcttcgatct cttttaatcg ataacttcct gttttcaacc
    15781 cggaaattga aaaagcgcca tttttatcgg aaatcaggtg aagcgcatct gattcgttct
    15841 ttgtccactt gtatccgttg gctgtttgct ggaggtaatt tcctgctttg tttttaataa
    15901 ggaatactgc gccagctaat cgtttatctg gtttatttgt ctcgattttg acaaattgct
    15961 tactccctgt atatacagag actttctttg tactgacacc aaaaccaaaa tccagtgttc
    16021 cgttgttgtt gatttccttg ttcgcctgtg ctgtactgct tagctgcatc tgataagaaa
    16081 tggtcagttt ttttccagca tatttttcca acgaagggat cgaaaataaa acatcaaatc
    16141 cgtgaggttt cttgtgtatg acaaagcctt ctgtcagctt ttctccattc gatgaaatcg
    16201 ttaaactatt aggtaaaaac gttaatgctt catccgcact atctgacaat tcgaactttt
    16261 gatagtcaag gatatttaca ggaattgtcg tatgcaagga ataagtgatt ttttcaccaa
    16321 tcgtaaaatc atttctaggc tcttctaata ccttctcaaa aggtggatca gggtagtcgt
    16381 tttcttcatt tttaggatac aaatggatag ttgaaagttt ttgattgttt tgtccatgta
    16441 caggaagaac aactaccata ttctctgcct tttctttgac gacttctggt actttggatt
    16501 caatgaaaag atacgcttta tctctttttt cttgatcttt agcggccaga gaaaaagaag
    16561 cgataccatc ttcgttgtta agagttgtag ttgttgcttc tgcggtaaac ataccggaag
    16621 acgcaccgtt ttttgcgatc tctgcttgtg cttcttctac cgttttgccc ttttcccgta
    16681 gatggtaaaa agaatctgtg acatcataaa cttggaatgt gacaccattt aatcctcgat
    16741 acgtttgtaa taaagctttt tcttgtccgt catttggatg atttttcggc agttgcccgt
    16801 ttgggaaaag caatttgtgt aaagtaatct gaaccatttc tgtttctgtt tctgttgcta
    16861 cctgagtaaa agctggttta aatagtaaca agaagagaca catactaagc caaccaagtt
    16921 ttttcatatt tagctgcccc ctctttcttt tttcaaaaaa agataccaaa tagaaaacgc
    16981 acaaccaatc atccctacta gatagattcc taaacggcca attcctcccg tttcaggtaa
    17041 tggtactttt gcctgattcg tgatgtctaa agaaatctgg ttgtttttgg ctcctggtga
    17101 cagaacagat tcatgatctt gtccatccac ttgaattgac ccatcttcgt gtataactat
    17161 agtaactggc tcttttagac cttggtatcc ttctggtgta aaagtttcgg tcagcgtata
    17221 agttccaggt tttaaatttt cgaatagaaa ggtatctgtt tcttgtccat cttttggcga
    17281 ttctaagtcc atttctggtc cctgcagtct gaattttgct ccttttaatg tctgtccgtt
    17341 gtcttctttt ttgtgtaccg ttagatcaaa cgcttttagt cgattttgat gagtgagcgt
    17401 ccatacatta tttgtcatct cggtattttt tcccgtgtaa atgatttttc catatttatc
    17461 gatttggaat tcataatttc ccggcgaagt atcatatccg gcaggaccag ctgtttcaac
    17521 gactcgatat ttaccaggtg tgagtgaatc aaaactggca agaccttttt cattcgttgt
    17581 ttggctgtca attggctgat aagtaccatt tgcttctttt ttctgtaggg aaaatgctgc
    17641 tcctttaaga tttatctctt tgtccgtccc ttgcatcgca tacttacgta ctgctacagg
    17701 aacttcaaca aaaggatttt caatagtcaa ctggatcgta tcatcggaag tagtgactgt
    17761 ttttccatca atggttaccg taccatcaga agcgatcttg atttcccaag tagtcttttt
    17821 gcttaatcca tgcccttctg gagcttttgt ttctgtcagc gtataggtca tttctttttg
    17881 caatttgaca ttttctggaa gagaataggt tccgtcgcca tgatctgtta gtaaagtatc
    17941 aatagaatct cctgttaatt tgaaaacagc gccaataaga tccttttcac ctgtagagga
    18001 atttttcgtt attttcaagt ttaacggtgt gaattgttta gtatttttcc atgtcattgc
    18061 atctatttgt tgagaatcgt atcctggtac aggtaattct ttgattgttt ggtaactgaa
    18121 tgcttgacct tgattattgt attgaggtag tgataatatc tcttgataac tttctccgct
    18181 atttgcagat aatttgtcaa tgtctgcacg ttcccacgta tttgttgtat ctttagctgg
    18241 tttaatgatt cgaatatatc cgtttttcca agctgcagca tttgtcgtat gttcccgttg
    18301 aatctcaaaa gtaacttgat ctggacgatc agctagattg ttgtcaaatt cttcccataa
    18361 ctttttgatg tgaagactga ctccgggagc tttagcagat ggtataccga attcagctaa
    18421 ttcattggta tcaatgtttg gctggaaagt tgtcctgccg ttcatttgat accagaaatt
    18481 tggatggaag tcctcatttt ctgtttggat tctcacttgg taatggattt ggatttcttg
    18541 gtcttttcct aaatagatct gattgctctt gatggtattt ccttctatgg aaatagatgg
    18601 agtgaccgtt gtaggacttg tccccacact cttgactgaa agtgtaccag gctgataaat
    18661 gaatggttct gcgattggat catttatttg tccattgctt acagttgctg agatctgtac
    18721 agcttttttg gctaggtatt cagagatatc tgttgcatga tcagctgatt catagtaaag
    18781 atcgcctttt tcatctgatg aaaccatttg tcgcatacga gactctactt ctgcttttga
    18841 gagaccagct gccggatcgc tttgaagttg gatgccaaga ccatgtattt cgattcctcg
    18901 ttctttgagt gccatcgctt ctccgatggt tgcgataaac gtactgtcga ttcttctgga
    18961 tagattgttt tggtcaggtg catcatagat tcttgagatt agagaagtat ttcccggccg
    19021 atcttgcgta ttagaaaact gagttccgta ataattgctg cttgattgtg cgtgtacccg
    19081 ctgtacttta taggaaaatg ttggtacacc atccgtcagc aaaacgatca cttttttatg
    19141 tccatttgga acggatagca tgcttcctgc atctcttagt gctttttgtg taaaagtacc
    19201 accatttgtc cgtgaaggtg taatggattt tacttgattt ttcactgaat caaatgaacc
    19261 catctgtact gcaccgttac tgtagctata tccttcgctt gagtagccga catatcccat
    19321 attgattttg tctgtgatac cgctatctgc taaagtatcg acaaaacgat cgacaccaat
    19381 ctttacttca ccgatccgat tattgtcgtt catacttcct gaccagtcta cgaccaatac
    19441 gagatcaaga ggcgtgatat ctttttgtac atttccacga gcattcaaat aaacatcaaa
    19501 caatccgggt gtacttgttt cctttgcata ctttcgtatc gcatagtctg cttgattacc
    19561 ggttcctcca tattcgatat acgaatgggt ccgatcatta ggatctccat tccaagaatt
    19621 tataccatcc cattgatttg ttccgttttt attgccttga tgatcaagaa catttgtatt
    19681 tcctgtaggt tgccaactgg ctttaggata agtgccctga tcatctgttg tatattctgt
    19741 aggaatcgtt tgattagatg gcacactcac agctggcaag gataatgtag gcaatgtaaa
    19801 tgggctgaca gaattttcgt tttcttttga tgattcttct tctatttcgt ctggctttgg
    19861 tactgacaca tctgtatctt tcttattttc tgtctcatca ataatgtcat attctttatt
    19921 atcgacgatc agcttgtttg cctggctgtg gtaaagtgct ggatcgattg gaaaactcag
    19981 atcgatagtt tgtttagtgt ttgatgagat ttccaacgta attttcccgc tatttacctg
    20041 ataagcatag ccatctcctt cttttctttc cacatcactt aagtaatttt cgactagacc
    20101 aatttctttt tctatctttt cagcactggt gttattcgca tttagcttga tgttcagtgc
    20161 tttttttcca gactgatccg aaatgacttc agctgaaatt gtttctaaaa ctgttggatc
    20221 atctgtagtg tctgctattg cagataatgg cacaaagttt gataaactca atattatcag
    20281 catgaaaata actttcagtt tcttccctgt ggtcgtcatt tttcccctcc tttttctttt
    20341 tttctgtttt gacgtgaata gtgaaaatta ttaaaataaa caggcgttac ataattttat
    20401 ttattttaat atgataatta ttatacgaag ggcgctaaag ggaatcacac caattttcat
    20461 accaattggt ttcatttatg aagttgattc cttcgattaa tgagcgtttc attgaaagaa
    20521 aagggttgag ggagaaaaac atatgatcag gttgttggaa ggaaactata gaaagatgga
    20581 aaaaatcatt cgaattctgt ccatttctga caagtggatg actaaaaaag aacttgccaa
    20641 acgaattggt agttcagaat ctacatttgt aaggtatatc gaagagatca aaatccgctg
    20701 gggaacggat gtaacgattc aatcttcaca aaaattgggg tatcgtcttg aacatttgaa
    20761 tgtaccagct tatttaaatg ttctgatgga aatgtcgcaa agttctacaa acagtcgttt
    20821 attaaatgag attattgtca atccgggtaa aacgattgag tattattgtg aaaaaatttc
    20881 catcagccgg tcttctttta cacgtaagtt gaaacaatgc aatcaaattt tagaaagcta
    20941 tttattaaaa gtgatcgttg accaaggttt tcagttaact agccttaaaa gtgagctcca
    21001 gttgcgtatc tttgttacct ttttcttttt aagttactac ggaagacgca cgttgccatt
    21061 tccaatcgaa aaaaagaaga tcaaagaaat tatggtcaag aataattgtc agatgaattt
    21121 gtttcaggaa gaaagcccat atgagcaatc tttctttatt atgtattttg ttgtgcatct
    21181 catgagagaa tcccaaggaa ctccatttga atggaaagct ggagactctt atgtacagtt
    21241 gataaatatc gataaaaaag acttcttaat cttgaaaaat ctcttaggaa aattgaagta
    21301 cagcagctat ataaaagtag tgaactattt tgtcaatacg attttttcag aattttcgga
    21361 gaaagaaaaa aatgaaatca gtgagcagat acgaaaaaat atgtcctact gttcgttttc
    21421 atccgctgat tgcaaagaaa aaaacgaaga atatgttgtt caattattaa cgcaaattta
    21481 ttttttctcc cgtattttcc catacgatac gactcatttg acgatccgtg caacttactt
    21541 tgcacagcag atgcaacacc gccagatacg tctatatgct ggatttcgtg cagaacttca
    21601 attttattcc actctactga atcagaatct gatttattat ttatctagct atatcttttg
    21661 gatgcttatc ggtaatcctg aaattcacca ttatacttct gataaaaaaa tactgattat
    21721 cagcgactta agtcttcgac atagtcaata tattgaagaa tatatcagtg atattcttgc
    21781 tgtgcataaa attcactctg agacaactgc tattactgaa gatcagctgt cgaaatacaa
    21841 tcttctcgag tatgatctga tcgttacgaa tcaacccatt ttgaatgccc atgttcctca
    21901 tatattgatt gatgatagtg tgtcattcgc aaatgaagaa gaactcatcc gcttattcga
    21961 gttatgaata atcaaaaaac agctatgtat aaaccaccct gataaaaatt tcaggacaaa
    22021 tacacatagc tgtttctttt tattgtattc cttcagcaat ccaacgagat ttatagcgaa
    22081 agtcttttaa caactcttct tttgtccaat tatttgcaat gagatccgtc aaagtgttca
    22141 ttacgagggt catggaaaag tataggaaac taactgcttc ttccgacgat tgaatggaga
    22201 aatgattgat tcttaataat tccagccatt tttcgttcat tggagaatca gccgatagtt
    22261 ctctacgttt ggttgtctgc tgatcatttc gctgggttaa caagtataac ctttgccaat
    22321 agatactttc gtgatctaag tcgcagcacc actctaaata tttttcgatc cccaaaaaga
    22381 aatgttgttt gttttcgcta atgaatttta agatatcttg atggactatg tgagaactgc
    22441 ttttgatcgt atacaaataa gcatcttcca aatcttgaaa atatttataa aaagcaccgc
    22501 gcgacatccc catcttgtca acgatttctg agactttgac ttggctgatg tgctgtgagc
    22561 aaaatatctc cagtaatagg tcatcgattc tctgtttctt tgcgttcgat aaattgaaaa
    22621 aagtttcttt tggcatccac atctctcctt tatctctgat tatacgtgga tagcccttca
    22681 ttgtcatgaa ggattcattc cttctttttc tcttcttctg tcaagtaccc atcttccatt
    22741 cgataaacgc gatcactcca ttgaatcata cggacatcat gagtaaccat aatagtcgct
    22801 ttttgtttct catgagcttc tttaactagt aatttgacga cttcatatgc atgatctgta
    22861 tccaaactag ctgttggttc gtctgctaag atcaagcttg gctcattata taaagcgcga
    22921 gcaattgcca ctctctgtct ttctccacca gaaaggtctt taggataact atttttaagt
    22981 gactggatat ctaaagattg cagcaaggaa tcgatttttg ttgaatcttc tttttttgaa
    23041 gtaacttggt caactagttc aaattgttcc tttaccgtta aaaaaggaat caaattagag
    23101 ctttggagaa taaatcctat ttctttaaag cgtaaatctg ctcgtttttt ttctggaagc
    23161 tcagttagtg agtgaccgtt gatcactatt tctccggaag tcggtgtttg caatcctcct
    23221 gaaatggtaa ggaaagtact tttacctgaa ccagaaggac caatgatgct gacgaattct
    23281 ccttccttta cataaaaatc aattgctttc aaagctgaga cctttgtatg accagaacca
    23341 aatgttttgg taactttttt catcgttaat atttttttca tttttattaa cctcctattg
    23401 ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa caatccgcct agcattgcga
    23461 caatgatcaa aatcccacta tatagcaacc attgtgacca aaaaacagca aaaggcatcg
    23521 cgcttggtaa aatcagacta gtcaaataag caaacaaaag agcaagcaga acgcctacca
    23581 ccccaacaat aaaggactgt gctactaaag atttagcgat gaagaaattt ttgatccctt
    23641 gtgctttcat tactccaaaa atagcagtct tctgaagtgt catcacgtac atgaaaatac
    23701 cgaccacggc agcaaccact aagaacaaaa agtatatcat cgcatttaag gtcatatttt
    23761 gggcagaata gccaggaata ctttcaatga atgtcccaat agttaataac tgtagaccct
    23821 tcgatgcttt ttctttcgaa attttagctg aatcctttat tacgatccca ttgatcggct
    23881 gttcttcttc agaagcaaac ggctgttgtc catacttcaa agcagtccac gtatctaggg
    23941 acgcatagat gacgggagaa actgtgtaat aagtttcagg aaagataccg acaatcgtca
    24001 aaggatcgtc gtaactgcca attttgatcg tatctccaat tttatagcct gaatcagcca
    24061 gattttgaga gatgatgatt tcattctttg ttttaaattc atgccctttc gtgagttttg
    24121 gtaataaaaa tgcttctttc gttgtgccga aaactgtaac attttctttg ttttttcctt
    24181 tgattgctcc gctgtacaat ccgattggtg ctttctctcc tccttcaatg tatttcagat
    24241 ctcctcttgt taattgtgaa gctgcaaata cttgatttgc atcctccgac aaaacaatct
    24301 cttgtgcgtc ccaatcatcg attgcttttt taaactcttc tgaaagtcca ttcgctagtc
    24361 ctgacaacat gaatacgaca tacgcaatca agaacataat accgacgatc aagccatatc
    24421 gtaatttaga atagcgaatt tctttgatag ctagaaacat ttgtttttcc tccctatgat
    24481 agattgatag tgacaaaatg tcacttgttg aatagtgtaa ctctaaagtg acaatttgtc
    24541 acttgtgttg tttttttctt atcaataaat tgtctaagac atctttcgat aaaaccttaa
    24601 catgagtcat aaaaaattta tttaagcctt atttatgctt tccatgataa actaaagtga
    24661 ccaaatcaat gaggtgggaa aatgattaaa ctattagtag tagaagacga taaagtatta
    24721 tccaataata taaaagaaat actcgctgca atcggcgaga taacacaagt ttatgatggt
    24781 agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc ttgcaatctt ggatttgatg
    24841 atgccggaaa tgaatggata tgaagtgtta gcaaaaatac ggaaagaaaa aatacagact
    24901 ccagtattga ttttgactgc aaaagatagt ttggaagata agattgaagg atttgaacga
    24961 ggtgcagatg attatctaac aaaacctttc tatcgagaag aactgatcat gcgcgtgaaa
    25021 gcattattga aacgctcgct aggtctcttt gatgaaaatg ttctgaagta taaggatatc
    25081 agttgtgatt taaaaagcaa tgaagtcttg tataaagaac aagttttgcc catacaagga
    25141 aaagagtttg aattgctcgt ttatttttta caaaataagg gaatcatttt gacaaaagag
    25201 cagatatttg accggatctg gggatttgat tctgatacga cgattacagt tattgaagtt
    25261 tatatgagca acttacgaaa acatctgcga agtactggat tggataaaga aatcaaaacc
    25321 ttacgtaatg tcggttatat actgcaaagg gtgtgatgca atgaatggag atattacacg
    25381 aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca tttgctttta tcttttttct
    25441 tttgggattc attactttac agatattaaa ccaatctgca tacagagaaa cagatctgtc
    25501 attagagaaa ataaattttg attctcgaac agtacaatta gagatacaaa gatatcagca
    25561 aggcgatccc tttttaaacc cgactcaaac ggaacaaaac agaacaaaca tggaacagcc
    25621 attaatggaa ccatcaaatg accgttttaa cactcaagtg atcttgtggt ctaaaaatgg
    25681 tgagatattg aataaagcag ctattggtgg gcgtttcaac caaatcaaag aattaaaaat
    25741 ggatacgaaa aatctgaata ccatccaaaa tattgaatta aatgaagaca gtggctcatc
    25801 tgccctctct ttccgttcga ttacgaaaaa agcggagacg aatcaagatg gcgttgccta
    25861 catccaaatt ttagaaaata caaatcaaat caagaattca ttagcgactt ttcgcacgat
    25921 tatgattttg tgtatgattg ttttttggat tctctctatt ggtatcagtt attacttgtc
    25981 tagtctaagc atgcgcccta ttattctttc ttggagaaga caaaaagaat ttgttgagaa
    26041 tgcttctcat gaattgcgca caccgttaac gattattcaa aatagtttgg aacatctttt
    26101 taccaagcct aatcatacta ttattgaaga atccgaaagc atcgctcaag cattgtcaga
    26161 gacgaggcgc ctcactggat tgacttcgga tttgctgaca atcgctcgga atgattctga
    26221 tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa tatatcgaac aacttgttaa
    26281 accatttcaa gagatggcca tcatagatgg aaaagagttt attctccata acgatgcaac
    26341 attagaagta ccaattgatg aaaaaaaaat ccatcaagtt ttggttattt tgctggataa
    26401 tgcattaaaa tatacgaatc cagaagataa gatcattttg gattcatctg ttaaccataa
    26461 agaatggctg attcacgtaa agaatactgg cccttctatc agtgaagaag atcaagcaag
    26521 gatttttgat cgattttatc gggaagatca atctcgggcg aaagagactg gcggatacgg
    26581 gcttggcttg gcaattgcaa aacaggtaat cgaacagcat catggacaga tttctgtatc
    26641 caattatctt ccacaaggag tcgattttaa agtccaattg ccgttgaagc ctaaaaaata
    26701 atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc acagcccttt tttgttgcga
    26761 ataaacggtg ttccagaagt agcttcttcg gaaataagcc gaaatttctt taagctgacc
    26821 acttctgtca caacctcttt agcttacgct tacgattttt tattgcggtc ttcaatgtgt
    26881 acatcaccgc ttgtgtcaat atccaattct tctcggctaa catcttctgt cactttttca
    26941 acatcttcat gttcttcttt atgaatccca acttcttcac gaataactgt atgtttgctt
    27001 acatctactt gctcttcttt gattgggata gtaatcgtgt catcttccaa tttttcgtct
    27061 gttccctgtg aagattgatc tgttactggt ttgcgttcga tgacgatttc ttcacgtttg
    27121 accggtactt ctaccgtttc agtatcgttg accacatgtt tgtggatgtc gacttctcca
    27181 gttgtcacat catgtgtatt tacatctaat ttttcttctt ttagttgaat tgtatcatca
    27241 tctagatttg gtttgcgatc gatgtcttca gtcttgcttg tacccattgt atcagtctcg
    27301 ccaggtacgt ccatcggatc agttcctggt gtcatattca atggatccgt tgtattttga
    27361 aagcctgtcg tgccacctac gtccattgga tctgttccag tgccagtttc taatccagtc
    27421 cccattgtgc ttgctgggct aacagtatct tgtgtatcca ttgtatcttc tgttgtttct
    27481 ggctgacgat agccttttac tgtgatgaca aattttccat cagaaatatc tcctctatat
    27541 tgatataaag gatcattttc tggtgtagcc gtctcagaat cataggtatc aaaagaaaaa
    27601 gtatctttta ttttttccca taaagaacgg tcttcttcac catccatccg attttcatct
    27661 gtttcaacat ctataccaga caatccgtca aatcctagtc tagatgctgc ttcatcagta
    27721 gtataaataa cgatatcatc tcgttcatac ccttcgttac gcaaccgttc aattactgat
    27781 aatgcttctt ccctcgtact atagcttccg atgaccgtta aatttttctc ttccatgtaa
    27841 attcctccta gcaaatagat ttatgattgg tatattgtta cttataaatt tattctatag
    27901 gagacgattg ttttctacaa atatttagat tggaatttga ttaaaacaat ttaattgaaa
    27961 tttgaaaaca tttatgttaa tgaaagtcat attctaataa aatgtcattt gtttctatat
    28021 gaatttttat gaaaaatttc atataatttc cactcgttgt gatggtttaa tttatccaat
    28081 ccatcaataa gcaaacaact tatataaaaa aggattaatt ctcaatataa ttgctcctac
    28141 catagaataa atcttcatgt aaagtaatga agtaccaact atttctaata cttctttaag
    28201 aattatccca acaatatcgt tagacaaacc attattgaat aaatggagag ccaatcgatt
    28261 ctgaaaaaat gttttctaat ttttcggaaa taaaagaaag agcctcctgc cattagaacg
    28321 aaccaaatcc atttcattgc tcatgaagta accacatagc taaaaagagg ataaatcaat
    28381 taaaagatca aaagaatgac tgcactaata aaataactaa aaatgtatct tcttacttca
    28441 tatcttttag ctagtactta gttaaaaatt tgcttcctaa gatcaagaca aactgtctat
    28501 cttcatttga tgaaatgcgg taaaatatag aaaatgaaca ctattggagg gaatttgtaa
    28561 tggacagcaa aactagaaaa caactgcaag aaaagttaaa gcaacgtaca cacttgctcc
    28621 cttccgtctt tcaacagttt ttagaagaaa atcgttcttc cctttcatta ggcagtcgct
    28681 atgaatacgc aaaagatttt catctgttta ctgattattt gagaaaagta ttttctgaag
    28741 aagtggatga tcagctgatt atccatttag aaaaacaaga ttatcagaat ttcttagcta
    28801 aaatctatcg acatactcgt tcgttccaaa ctacagcgaa ccaagaaacg gagcaattgt
    28861 ttgaaaatag ctattacggg atttcaagga agcaatattc attaaatcat tttttacgat
    28921 ttttgtatca aaaaggactg attgaagaag agatttctct tttgggtaca agtttaccgg
    28981 aaacaaccaa agcagctccg cgatatatcg atgcagaact tttcaaagat tttgagcatt
    29041 tgtttatccc gattgaaggc tttcaaacaa gcagagaagc tagctttgaa gaaaagaata
    29101 gaccgaggaa ccttgcaatt actgccattc ttctgtattg cggattaaag atccatgaag
    29161 tagtagaact taaacgtaaa gatgtatctt tgaccaagca gattcttaat ctgaatagaa
    29221 aagaaaacag actgaataaa gtaccaatac caaaagaagc gatgccattt ctggagaact
    29281 attttaagtt aacagcacaa aatacggacg aaaattcttt tgtttttcgt tcctcacatg
    29341 atcagcaaat ttctcctcgg actattcgcc aaattttgag caaaattaca gtatataaaa
    29401 acgtatcgcc agaactatgc agaaaaacat atcgttatta tgcagaacta tatttagatg
    29461 atgctgacgc ggtaaattat ttatgtggta atcgatacct ctcatcccat tcttttcaag
    29521 atatctggga aaagatggtt gatttttctt atcacgaatt agcggcattc gttcgggttt
    29581 cttccatata aaacaaatgt atgatctttt tttcacaata aactatgtta ttatataaag
    29641 gattatgtta cgattatctt gaatttgtat attacttaac aaaagataat ggagtgtgaa
    29701 tgatggagac cataacgtat tcccctttga acttatttac gaattttcaa gaagctgcag
    29761 ctaacttttc cgagaccccg attatctttg atgagccact ttcagctttc ccagaattag
    29821 gacttgaaac aacttacaaa aaatgctgcg aaagtgtgtt gaaacgggct tatcaattgg
    29881 ctcatcttgg agtgaaatcc ggagacaaag ttattattta taagagttcc gcttttgaca
    29941 cataccttct agctgtttct gtgtcttacc ttggtgcagt gcctgtcatg acttcttatc
    30001 atctaccaac aacaacaatg gaagtattta tcgatcgttt agaagaccct ttcattttat
    30061 ttgatgatga gacaaaaaag cgagtgagag aaatttcaaa tggaacaaaa tcaaaacaga
    30121 ttcctattct ttatttaatg gaacaacctg ctttgtctgt ttctcaatca tttttagaca
    30181 aaaatgaaat ttcttatatg acccatactt ctggtacaac tggtatccct aaacttattt
    30241 gtcattctgc tcattctatg ggatggcgga cgaaatggca aaaaacagtt ttcacaaaaa
    30301 tttcggaaaa gaaattaatc gcttttcaca tctctccagt gcattccaga ttcaatattg
    30361 gtatctcttc tttgatgtcc atgggatttc ctatgatgcc tttagcaaac gcacaaagtt
    30421 caaaagttgt tcatatgttg gaagctcacc ggccaattgc actagagacc catccgaata
    30481 actttgttca gtggcgtttt actgctaagg agcatccaga agctttttcg ggtatccgat
    30541 actatcactc cacttttgat gctataaaca accagacaat gaagacattt ttgcgaacgt
    30601 cgatggcaca ggatgcggta tttcttcaag tatatggcca aagtgaatgc ggaccaatga
    30661 ttcttaaggc tcatacacta gagagtttga aaacaagtga tgcaagagat atgggcgttg
    30721 gactggaaga catgacaagc gctcggatta cagattcagt tgggaatgtc ctacctgaaa
    30781 acacagatgg tcatatacaa ttactgtcta aaggacgggc tttgacttat tacaaggaag
    30841 atgcacggtt ccaggaaaat gtttatggcg attggtggga tagcggagat tacggtttta
    30901 tggataagca tggccaccta tttttgaaag atcggcaagt ggatttgatc gaaacgatca
    30961 atagtacatt agctattgaa gactttttat tagattcgtt agattttttg gcagaagtag
    31021 tgattgtaag ggataaaaat cattctccac aaccgattat tgctttagct cctgataaag
    31081 aaatggattg gaatcgatgg tgggagcaag tccatgaact tcctcgcttg aacgtcccta
    31141 tcatccgtga ttttgatgca atcccgcgaa cagctacaat gaaagtgcaa cggcttcaaa
    31201 tcgagaaaga attaaaatca caaaagtcct aactatttaa ggaaagaaag atccattttg
    31261 gtctttcttt tctttttgtt tttttaaatt ggatgagata aaaatatgcc aattggctgt
    31321 ttttcttttt tgaaaatttc gttatactct tattcatgga ggaaaatgtt atgaaaagaa
    31381 tcttgacaat tggcggttct gatccatttg ctggtggagg aatccaaaca gatttaaaaa
    31441 cttttgagaa ttttggtcta ttcggactaa gcgccttgac ttctataggg gcactagata
    31501 ttaatgagcg atttatgctg gaatctatat ctttggaatt gcttaaacga cagttagttt
    31561 cgattgataa aatgacttca ttagatggta tcaaaattgg gttattgaat tcaagtgaag
    31621 caatcatggt tgttcgagac ttcataaaaa caaaacaaaa tatcccaatc attttagacc
    31681 ctgttttggc attcaaagaa actgaagctg tgatcaatca attgtatatt tcgcagctca
    31741 tcgaagaact ttttccactt gttacgatga ttacgcctaa tttggcagaa gctgctttac
    31801 tttctggtca agctcttccc tcttcgttaa cagaaatgat ccatctcgct aaagaactca
    31861 ctgcttcagg aacaaaaaac attgtgatca aaggcggcac aaggatggct ggagatgaag
    31921 ctgtggatct tctctattca attgatcatt atgaggtatt ttctcataaa aaaatcgaca
    31981 ctacaacagt aaacggggcc ggttgtgcct tttcttctgc aataacagcg aatcttgtgt
    32041 taggggcaga ttttaccaaa gcagtagcag aaagtaaaaa atttgttgcc cattgtattt
    32101 ctaatggcgt gctgatgaac gatcataccg gaagtgtctg gtctggcggt attcaaaaag
    32161 gaggaatgag aaatgaaaac taagcaatta acgatctatg ctatgctgat tgcgttgaca
    32221 gttgcccttt cattaacaat tttgatccct gtaccggcta cgaatggatt tgtgacatta
    32281 tgcgaggccg ggatctatac cgctgctttt ctttatggtc caacgggtgg attagttgtt
    32341 ggtgcttcca gtggtctatt gatcgatttg atttcaggat atccgcagtg ggcagttttt
    32401 tcattagtga tccacgggtt acaaggatat gttgctggtt ctttcagcaa atcttctact
    32461 cgtatgtggg gattagggct tttattagga agcttagtta tgatcatcgg ctatggtatc
    32521 gccggttgga ttttatatga ttggccggct ggagtggctt ctataccagg aaacatcttc
    32581 caaaatctga ttggaatggg acttgccttt cctataactg caagtcttca acgtattcaa
    32641 gcacggaagc aatattaatt gaaaaagaag gttttagaaa atgaagttgt cagaaaaaga
    32701 attaaaagac caattgactg aaatagttaa tgacatattg gctgaggcac acttgaaaaa
    32761 aggtgatctt tttgttttag gatgcagcac aagcgaagtc gttggaggac atattggaaa
    32821 aaattccagt gcggaagttg gtcaatggat catccgtacg ttaaaagagt tgttggatcc
    32881 taaagaaatt gcgctagctg tccaaggttg tgaacatttg aaccgagcat tagtagtaga
    32941 acgaacagtg gcagaagcaa aaaatttcga aattgtctct gttgttcctg cactacatgc
    33001 tggaggtgct tgttctgttg ctgcttttga ccagttcaat gatcctgtcg aagtggaaca
    33061 cgtagttgga caagccggta tcgatatcgg tgacacatca atcggtatgc atatcaagca
    33121 tgtccaagta cctgtcagac ctcgtttgaa aacccttgga caagctcacg tgacagccct
    33181 tcgctctcgt cctaaatata tcggtggtcc tagagcgaat tattgaaatg agtaattgac
    33241 agagcaatct gaaaaacttc ctcgcaaagt ccaactaatg aactttaacg aggaagtttt
    33301 acttgtttgt tttatataac cgagcttcat aaggtgctag tatgccttta gcttcaaatt
    33361 ctccgctaga ggaatttgac aagagtaatg tccaggactg atctgcttct tttggaagtt
    33421 catagtcagc aagtgattct gttaagttta ccataataag atattgttca tcttctaaat
    33481 atctttcata gacataaagt tgtggatgtt catgaagata ttctttataa gaaccgtata
    33541 tcagaccttt attggtttgt ctaatgcgga tcatgttctt atagaattgc aggaccgact
    33601 ctggttcata ttcttgttgt tctacattga tcgttttggt atttggatta gtgatcagcc
    33661 aaggttcatg tgtagaaaaa ccagcatatt gttccgaagt ccattgcatg ggtgtcctgc
    33721 tgttgtctct cgttgtttca cgaatgatat ccaatgcttc ttctcttgtc ttaccttccg
    33781 caagcagcct tttataaagt cttttcgaat ctactgcatc aacttgatcg atcgattcaa
    33841 agggcatatt cgtcattccg atttcctgtc cttgataaat aaatggcgta ccttgcaaaa
    33901 agaaatacat catagcgatt gcttttgcag acatagccca aaaatcggga cgagtatctc
    33961 caaacactga aacggcacga ggaacatcat ggttttccat atataaagcg ttccatccga
    34021 taccatccaa tgaagtttgc caagcgctta atgcatgttt caatttaagg acatctaatt
    34081 gcccttcttt ttcttgctgc cagatatgga tatgatcaaa ttcgaatatc atatcgaaat
    34141 aaccgtcttt ccctacccat tgtggtcctt cttctgcagt gacaccactt gcttctccga
    34201 cagtcatgat atcgtactct tcaaaaacat gtttcaaatc ggtcaaatgt tcttcgattc
    34261 ctgagacatt ttgaaagggt gagaaagggt tctctgtggc ttttactgaa tattcatctt
    34321 tttttacatg agaaatcgca tctaaacgaa atccatctat ccctttatcc aaccaccatc
    34381 ggatcatatt gaatagttct tctttcaatt tctcgctttc ccaatttaag tctggttgtt
    34441 cttttgcaaa aacatgcaga taatactgtc cagtttcatg agaatactcc caagcagaac
    34501 cgccgaaaat cgatgtccaa ttattcggtt ctttgccatc taccccgtcg acccaaatgt
    34561 aataatcgcg atacgggctt tctttagatt ttttcgattc ttcaaaccat tgatgctgat
    34621 ccgaactatg gttgatgaca agatccataa tcactttgat ccctaattca tgtgcttctt
    34681 ttagcaataa atcaaaatct tccatcgttc cgaatctctc ttctatatct tgatagtcac
    34741 ttacgtcata accattatcg acaaatggag attgatagat cggattgatc cagataaagc
    34801 cgataccaag atctttcaaa taattaagtt tttcacggat cccattgatg tctccttgac
    34861 catcgccatt gctatctttg aaactagcag gatagatttg atagcctact gcttctttcc
    34921 accatttttg ttcttgtatc atatcgtttc actcctttaa cggtccctct tgattataac
    34981 cgattctatt ttagaagtat ccttgttacc ggtaactaga aacgaaaagc tcaacgaatc
    35041 tgctaagtgc gttcgttgag ctttatttta tcatagatag cgggcaatca gttgaatcat
    35101 ttgcttggga aattcattca agtcagtaat atctacaaaa cgactttcac cgtagatcga
    35161 cttgatttct tctttgtctt ggccaatagc tgcggagaca aataggattc cttgacgttc
    35221 atattctttc aaaacagact ggatatcttc ttttgctttt ttgcctgtat aatccggcaa
    35281 tgcttttggc tgaccgtcac taatattaag caacagtttg gttgttgcgg attgtctgtt
    35341 taatttttct gctgctaaac gaagtgcagc accatcacga ttgttttgcc ttggtttcat
    35401 agtgactaat ttttcattta accattgaaa tccgtcttca aattctttat aagaaaacaa
    35461 agaagtcttt tctcttgacg aacggtctgc tgtatctccg taaatcaaca atgggatatc
    35521 gactttctta gaaaattcgg caatggcaat cgctgctttt tttgccgctt cgatccgatc
    35581 atcccgtatc attgacccag attcatcgat ccgtacagct accgctaaag aaggttgctc
    35641 atgtggcggg ttctttttgt caaaatttct caaatcacca tatgctactt tgcttgcatt
    35701 gaaccgctga ccttcgtatt ttcctttttg gtacgtctct gtttgttcat tatccagtaa
    35761 ctctaaaatt tgccggctaa gtgattctac tattggcatt acttgcatgt gtagctcacg
    35821 tgcttccggc gtttttcctt ggaaagctgg tcggtgaacg atgagacctt ctttttcatg
    35881 aatcgtttcc ttcatgattt gtcgcgcttc tttattcagt tgcttcttga tttccttatc
    35941 atgtgcttcc atgtcagaag cagacatttc ttgagaatca tctcctgttt gttgttcttt
    36001 ttgatttttt ccttcgatat cgctactgcg ttctttttgc atttgaggag gaatctcgtt
    36061 tttttctatt gtttcttctt gtttctcttc tccctcattt ggttcttcag cagacaacga
    36121 tttcttttca tctaagcgca gaatttcttc agcattttca gtgatgtttc gttcatttgc
    36181 ttcttcggcg ctaatggttc tttcttttgt atgctcagat tgaggatgac cccatttttc
    36241 ttttctcaat atgtcctgaa gttccagtag caatccactg tcagacaaag cttgttcaag
    36301 aattgccaat tcatctgaat cagtggataa tttatacaaa acttttggat aaatcgactg
    36361 gagtaaatct tgtccttgct tataggtatt tgcccaataa aaataagaac gcattcctgc
    36421 tacacctttg atggcgttag cttttgcagt ttcatccaac aaacggataa tctctgccat
    36481 ctttaagaga agttcttgtt tttggatcat agttttcgcc atcgcacgtt ctgccatgac
    36541 ttctaaggca ggaagatcca ttttttccgc atgttgcatc cgatctctta aagattcatt
    36601 taatgggcga tttccttggt aatttcgatt tgtagtgatc acaatgacgc aatctggatg
    36661 gcgacgaatg attcctgtcg gtaaattcaa catcccattt ggttcgagcg ctgagttaag
    36721 tgccacaaga acagaggcat ctcggatgac agtcggctct tgaatctcta ataaatagcc
    36781 tttttcaatc gctcgaacga tttcagaagg gtaatagctg taatggattg catctggata
    36841 atcagcttcg atcctttgaa ctagctctgc taatttttct ttagcagaaa aaggatcaat
    36901 gccgtaatgg ttctggatca gtgtcagtac agcttcaagt gtatctgttt ggtaaatagc
    36961 ttctagtaat tcttggtctt gttccttatt agaatctact acaggtaata aggctccgaa
    37021 aacatctgat ttgtccatat ctgcaaaaca ggtcactttt gtatagggaa gttgcagatc
    37081 agcagatagt gctttggcta attgtgtctt ccccgacccg gcatctcctt ccaaaagtac
    37141 gttactgatt ttcatttcag gatcttgcca attccctttg atttcggagg cgatccgcca
    37201 ttcttctgca cttgtccgat ggcttttcgg tttcttccag atcatttgct tttctagccg
    37261 tgaaaaggtt cggttttctg ttagtgagaa attctccatg cttctcttcc ccttttgatc
    37321 atctctgtct attaatatcc tagctcatct aaaagacgtt ttaaaatacg gaatcgttcc
    37381 ccaatcagtt ctccgtcttt ttgtagtgta tcgtaataca aaatgatgtc ttcatcgatc
    37441 tttggatttt tggtatctac ttctaccgtc attcggttcc cttgtaatcc aatgatgtct
    37501 acgactggat tttctggatc ataaaatttt tcataacgat aagcatcttg gaagtataaa
    37561 cttccttgcg cagctaagat cgctaaatct aaaatacgtg tgattggtaa ttcttccgac
    37621 tgtcgtgacc atttttctcc agtatgtcgc cataccttgc ctgagatatc gacttttcca
    37681 cgatcattcc actgagcaag gcccagtgaa agcccttttg catccatatt taatgcattt
    37741 cttccatcaa ctttatcata gtcttctaca acgattactg gtttatgttt taaatttgtt
    37801 ggtatttcca ttattttaac tcctcatcct tttattttta gtaaataagt aaattagtag
    37861 tttactcatt tactaaacta tatgcgacat tatagaggat tgaagtgagg attgcaaaga
    37921 aaaagagctt cattgataga aataaaaaca ccatctcgtt cttttacacg ctcattagta
    37981 acatgtagaa agtccaagat gatgttggaa tattt
//
LOCUS       DAYYDS010000017        37495 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000017 DAYYDS010000000
VERSION     DAYYDS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.017"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..315)
                     /locus_tag="ACHA02_000881"
     CDS             complement(151..315)
                     /locus_tag="ACHA02_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669251.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            610..1938
                     /locus_tag="ACHA02_000882"
     CDS             610..1938
                     /locus_tag="ACHA02_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8669252.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2140..2973
                     /locus_tag="ACHA02_000883"
     CDS             2140..2973
                     /locus_tag="ACHA02_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8669253.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2989..3726
                     /locus_tag="ACHA02_000884"
     CDS             2989..3726
                     /locus_tag="ACHA02_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8669254.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3881..4849)
                     /locus_tag="ACHA02_000885"
     CDS             complement(3881..4849)
                     /locus_tag="ACHA02_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8669255.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5081..5914
                     /locus_tag="ACHA02_000886"
     CDS             5081..5914
                     /locus_tag="ACHA02_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8669256.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5948..6787
                     /locus_tag="ACHA02_000887"
     CDS             5948..6787
                     /locus_tag="ACHA02_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8669257.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6813..7373
                     /locus_tag="ACHA02_000888"
     CDS             6813..7373
                     /locus_tag="ACHA02_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8669258.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7572..9293
                     /locus_tag="ACHA02_000889"
     CDS             7572..9293
                     /locus_tag="ACHA02_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8669259.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9458..10600
                     /locus_tag="ACHA02_000890"
     CDS             9458..10600
                     /locus_tag="ACHA02_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8669260.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10616..11827
                     /gene="thiI"
                     /locus_tag="ACHA02_000891"
     CDS             10616..11827
                     /gene="thiI"
                     /locus_tag="ACHA02_000891"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8669261.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12400..13047
                     /locus_tag="ACHA02_000892"
     CDS             12400..13047
                     /locus_tag="ACHA02_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8669262.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13176..13392
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13440..16085
                     /locus_tag="ACHA02_000893"
     CDS             13440..16085
                     /locus_tag="ACHA02_000893"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8669263.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16395..17708
                     /locus_tag="ACHA02_000894"
     CDS             16395..17708
                     /locus_tag="ACHA02_000894"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8669264.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17698..18351
                     /locus_tag="ACHA02_000895"
     CDS             17698..18351
                     /locus_tag="ACHA02_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8669265.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18406..18957
                     /gene="radC"
                     /locus_tag="ACHA02_000896"
                     /pseudo
     CDS             18406..18957
                     /gene="radC"
                     /locus_tag="ACHA02_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            19088..20173
                     /locus_tag="ACHA02_000897"
     CDS             19088..20173
                     /locus_tag="ACHA02_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8669266.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            20170..22062
                     /locus_tag="ACHA02_000898"
     CDS             20170..22062
                     /locus_tag="ACHA02_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8669267.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            22069..22446
                     /locus_tag="ACHA02_000899"
     CDS             22069..22446
                     /locus_tag="ACHA02_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFM8669268.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            22600..23382
                     /locus_tag="ACHA02_000900"
     CDS             22600..23382
                     /locus_tag="ACHA02_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFM8669269.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(23508..24239)
                     /gene="erm(A)"
                     /locus_tag="ACHA02_000901"
     CDS             complement(23508..24239)
                     /gene="erm(A)"
                     /locus_tag="ACHA02_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFM8669270.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(24297..24356)
                     /locus_tag="ACHA02_000902"
     CDS             complement(24297..24356)
                     /locus_tag="ACHA02_000902"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFM8669271.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            24748..25410
                     /locus_tag="ACHA02_000903"
     CDS             24748..25410
                     /locus_tag="ACHA02_000903"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8669272.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            25645..25788
                     /locus_tag="ACHA02_000904"
                     /pseudo
     CDS             25645..25788
                     /locus_tag="ACHA02_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            25938..26138
                     /locus_tag="ACHA02_000905"
     CDS             25938..26138
                     /locus_tag="ACHA02_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8669273.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(26392..28071)
                     /locus_tag="ACHA02_000906"
     CDS             complement(26392..28071)
                     /locus_tag="ACHA02_000906"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8669274.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(28073..28285)
                     /locus_tag="ACHA02_000907"
     CDS             complement(28073..28285)
                     /locus_tag="ACHA02_000907"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8669275.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            28678..30408
                     /gene="efrA"
                     /locus_tag="ACHA02_000908"
     CDS             28678..30408
                     /gene="efrA"
                     /locus_tag="ACHA02_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8669276.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            30405..32165
                     /gene="efrB"
                     /locus_tag="ACHA02_000909"
     CDS             30405..32165
                     /gene="efrB"
                     /locus_tag="ACHA02_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8669277.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            32260..32457
                     /locus_tag="ACHA02_000910"
     CDS             32260..32457
                     /locus_tag="ACHA02_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669278.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            32610..33095
                     /locus_tag="ACHA02_000911"
     CDS             32610..33095
                     /locus_tag="ACHA02_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8669279.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(33199..33792)
                     /gene="clpP"
                     /locus_tag="ACHA02_000912"
     CDS             complement(33199..33792)
                     /gene="clpP"
                     /locus_tag="ACHA02_000912"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8669280.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            33972..34499
                     /locus_tag="ACHA02_000913"
     CDS             33972..34499
                     /locus_tag="ACHA02_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669281.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            34590..35327
                     /locus_tag="ACHA02_000914"
     CDS             34590..35327
                     /locus_tag="ACHA02_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8669282.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            35331..36248
                     /gene="miaA"
                     /locus_tag="ACHA02_000915"
     CDS             35331..36248
                     /gene="miaA"
                     /locus_tag="ACHA02_000915"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8669283.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            36250..37479
                     /gene="hflX"
                     /locus_tag="ACHA02_000916"
     CDS             36250..37479
                     /gene="hflX"
                     /locus_tag="ACHA02_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8669284.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        12580 a         6174 c         7847 g        10894 t
ORIGIN      
        1 cctactgccg gagtaaataa agcttatgca aagaaaaaga aatccaatca cttgggtttc
       61 tttttctttt attattatga tgaatcaagc tttctgtttt tgtcacgtat gttgtaatct
      121 ttacgaaaga gtagagttgg ctactcataa tcaaccatac ttcccatcat cgctaaatac
      181 gaaataaatc agcattccta caccaatcaa agcacaagct gcaccaagaa aagggatgac
      241 attcccaaag ttatctgtat tcataagtat gccaccaccc acaaacaaaa agccgccagc
      301 aatcaggttt ttcattttat tcatctcctt tcttacgtta tagtatttcc ttctttcaat
      361 gagtattttg aaagctatct tattgtatga tgttcttcta ggcctcttct ttattataca
      421 ggccattttc gttatctcat agaaatattc ctttttatct gaaaactatt gtataccgta
      481 tgacattttt cttcatgaag ttgtaagtat attctcattc aaatgaaaat ttcatcatag
      541 aagagattgt atttctaaat tcttaagagt aaaatacgaa gtataacttg gatttggaag
      601 gatgattgga tgaaagctat tacgcctgat atgacaacaa aatttaacaa acagtttcaa
      661 gcaaatatca aacagcaggc attgcagcgg gcagttgtga agaacggaat tactgcttct
      721 gctgaaaatg tttcagcaca tgtggagaat acaccggtat tttctataga tttggctacg
      781 gggaaagtgg ctaatcaaat gcaatcgggg cgttgctgga tgtttgctgc actgaacaca
      841 ttccgtcata agatgctgaa tcaattccat ttaaaagagt ttgaattatc acaaaattac
      901 acttttttct gggataaata tgaaaaggca aattatttct atgaaaacat cattgcaaca
      961 ggaaaagagg caatcgatag ccggaaagta gctttcttac tcgctactcc tcaacaagat
     1021 ggcggccaat gggatatgat cgtttcatta ttccaaaaat atggcgttgt gcctaaatcg
     1081 gtgatgccgg aaagtgcgaa tagttcaaat tctcgagatt tgaataacta cttgaacaaa
     1141 ttactgcgta aagatgcagt agtgttacgg aaaatggttg ctcaaggaga aacattagac
     1201 gctattgaag aaaagaaaga agaaatgcta gaaagcattt ataacttctt agctatctca
     1261 ttaggaacac ctccaaaaga attcgatttt gagtaccgcg atgaagaaaa gaactatcat
     1321 cttgaccgag gactgacacc acaaattttt tatgataaat acatcggtgt aaaattagat
     1381 gattatgtta gcgtgatcaa tgcaccaaca aaagacaaac cattcaatcg ttcttataca
     1441 gtagagatgc taggaaatgt cgttggcggg aaagaagtca aatatctgaa tgttgatatg
     1501 gaaactttca aaaaattagc gattgctcag cttgaagaag gagaatctgt ttggtttggt
     1561 tgtgatgtag gtcaatcttc tactagaact agtggcatca tggccttaga tgcttattca
     1621 atggatgatc tatttgatac ggattttaca atgactaaag ctgaacgttt agattttgga
     1681 gaaagtctga tgacccatgc gatggtattg accggtgttg acttagtaga cgggcaatca
     1741 acaaaatgga aagtcgaaaa tagctggggc gaaaaagttg gaaagaatgg cttctttgtt
     1801 atgagtgatg cctggatgga cgaatatacg taccaaatcg ttgtaagaaa agatttattg
     1861 acgaaagagc aattaaatgc gttcaatgaa gaaccaattg ttctatctcc gtgggatcca
     1921 atgggtgctt tagcatagac gagtatttca atgaagagta tgtagtaaag ataaaaagag
     1981 ctggcttaag ccagctcttt ttatctttaa agtccattcc ctgtttttta tggtccctgt
     2041 gctataattc taaataaagg ataaggattg gaaataatcc ttttatcatt aaaaatatga
     2101 tgacgaagta gaataaaatt gggagtaggg gggaaagtca tggatatcca aactgtttta
     2161 tctgatctga aattaaatgg taaagtgatt ccagttgttg gtggcgatgt taatcagacc
     2221 tatcgtataa aaaccgaaca tcgtgcttat tttttaaaaa tccatccgaa tgtaaaaaaa
     2281 ggattttttg aagcagaagt agacggatta aaagaactgt cagcattcgt acgtgtaccg
     2341 gatacgtata tgctaggtga aacaagtgaa ggcgcttatt tgttaatgga atggatcgag
     2401 ccgggaaaag gggatcaacg agatcttgct gcagctttag ctaatctcca tcagcaaaca
     2461 gcgccacaat ttggttttcg taaagataac tatttgggta cacttgttca gaaaaattct
     2521 tttgaagaag attggtggac ttttttcttt aaagatcggc ttgaatccca gatttctttg
     2581 gcagaagaaa cgaatcgatg gaatgtacag cgtcaagaaa agtatctgcg atttaaagaa
     2641 cgtgtattga aatcggttga accaaaaaag atcactccac gcctcttgca cggagacctg
     2701 tggagtggta atgtgttctt tgaccagcaa gggcatccag tttttgtcga cccagctgtt
     2761 tcttatggga atcgggaaca agatattgca atgagccagt tattcggtgg tttccgaccg
     2821 gaatttttag atgcttatca aactatattt ccattagaaa agggatggaa agaccgactg
     2881 ccgatttacc agttatatta tcttttggca catttgaata tgtttggaga aagttacggt
     2941 tcccaagtag atcaattact agaaaatttt taagaggagg gcgtgttttt ggcaaaaaaa
     3001 tataccaaaa agcatgaagt ttcatattat gagtgtgaca tcaaccaaac gatgacattt
     3061 ccttcgatgt taggagtagt tatcaaaacg tctgaagatc aaagcgacca gttgggaaga
     3121 ggcagtgaat tcgtcaatag ctttggactt acttgggtca tcacgaatta ttccatggag
     3181 attaatcgtc tccctagagt cggtgaagta atctcagtaa cgactcaagc catggagtac
     3241 aataagtttt tctgttatcg gaacttctgg atccatgatg aagatgggaa agaattagtc
     3301 aaaatagaat ctgttttcgt attgatggat tttgtgaatc gaaaaatgag cagtgttaat
     3361 gaagagatca ttgctccttt tgagagtgag aagatcaaga aaatcaagcg tcaggaaaaa
     3421 attgaaaaga ttgattcggg cttcatgttg ccatatcgtg ttcgttttta tgatatcgat
     3481 agtaatcagc atgttaataa tgcgatgtat tttaattgga tcattgacgt attaggatat
     3541 gactttttga ctacgcatgt tccagaaaaa gtaattattc gatttgataa agaagtagag
     3601 tacgggaatg agatcgaaag ccattttgaa caaatcccta tcgaaaatgg tgtcaaaaca
     3661 aaacatgaaa tccgaatgca agaacagctg tattgtgaag caaatattat atggaaaaat
     3721 aaataaaaaa gaggttgtga cagaaggggg caacttcaag aaataagaag aaatttacga
     3781 aaattgtttc tcaaattttt gtaaattttc gacttagttt tcgaagaagc tatttttgtc
     3841 acagcctctc tttttaagtt tagctattag ataatgatgt ttagttttgg ataaacgaaa
     3901 tcaattcttc acccgacaaa ccatagttta cagcaatcaa tagttttaaa cgtgcttttt
     3961 gactattaat actgttacag aaaataatcc ccattttttc tagttctttt cccccgcctt
     4021 cataatcata aacaggctct gcgattccat tgaaacaacg agaaacaagt actacaggga
     4081 ttttttggac aagaagcttt tccaaagcag ctaaggcttg tggagggaga ttccccgcac
     4141 ctaaagcttc gattaccaaa ccgtctactt tcgtatgggc gatggcttcc aataagtccc
     4201 cttgcatccc agcatatgct ttaacgatcg ggatagtccc atctacagcg gaaatgtcca
     4261 gtcgctttgt ttctaaaagt tcctgcaaga ataaaattcg attcttagtt actaagccga
     4321 tgggacccaa tgtaggtgtt cggaaagtag caacatttgt ggtatgggtc tttgtcacgt
     4381 agcgtgcaga atggatctca tcgttcatga ccaccaaaac ccccttatct agcgcttctt
     4441 cacaacttgc gacacgaatc gcactttcaa aattataaag gccgtcactt ccaagttcat
     4501 tacttgaacg cattgcccca gtcaaaacaa ttggtaattt gttgccaatc gttgtatcaa
     4561 gaaaaaaagc agtttcttct aatgtatctg taccgtgtgt gatcaccact ccggatatac
     4621 ctgatgcata agcggacaag atacgttctt ttaataccaa catgtgagaa ggcatgatgt
     4681 gtggtgaggg aagattgaag atatcttcaa ctaataattc tacatgcttg gggattgtga
     4741 tttctgcatc taataatggg tttgccacat caggactaac accgcccgtg agcatgtttt
     4801 ctttcatggc gatcgttccg ccggtgtgta gtacaagtat ttttttcatt ttacagactt
     4861 ccttacgaat aattttgcat agccgttaaa tcttattgta tactatataa aagagttttg
     4921 ctatcagtga agaataacga atagcctaac ttatcataaa aaagctgacc ttattaaaat
     4981 ggaaaagact caaccttgac ctcctaatac aatggagtat ctggttgagt ttcttttaca
     5041 agaaagcagc caaggaaatc aatcacgaag gtggccggaa atgatcagaa tcggattaac
     5101 atcgtttaaa gaacatgaca aattaactgg taaaaagaat aacactcttt atgaatatgc
     5161 agggtttcta cccttagtgg aattagatac tgcctattat ggaattcccc gaaagtcctc
     5221 agttgagaat tggataagtg aagtacctga atcatttcgc tttgtagtca aagtctatca
     5281 aggaatcagc ggtcaaggag actggaagcc ttattatcag accgaagaag agatgattgc
     5341 gcatttttta gatgttatgt cgccaatggt agatgctgga aagatgtttt gctttttagt
     5401 ccaattcccc tcatcattca aatgtacgaa agaaaatgtc cagtacttgc gcaaacttag
     5461 aaagtggttt gagggttatc ctgtttctat agaattgcgg gattattctt ggtacagcga
     5521 tgaatttctt gaacgtacaa gaaactttat gtctagagcc gctttttctt tagccatcat
     5581 cgatgagcca cagttgctga atacaacgat tccttttgat ccatttgtca ccaatccgaa
     5641 ctttgctctt tttcgattcc atggtaggaa tgcgcaagga tggcaagcaa atgataaaga
     5701 gtggcgtaaa aaaagaacgt tatatcgtta ttcagaaaaa gaactgcaag aactagcaca
     5761 tgaagtcaaa gtagttgatt ctaagacaaa agaggtaggt gtgattttta ataataattc
     5821 tggtggtgat gcggctgaaa atgcattagc actgcaaaaa atattaaata tcaagccaga
     5881 tggactcaat ccaacccaaa tggatctgtt ttaagggctt attttattca agaaaggggc
     5941 gaagtctatg atcaaagcta ttttttttga tattgacgga acattggtca ccaatcgttc
     6001 aaaagcgttg gaatcaacaa aacaagcaat tgaatatgct cggaaaaacg gcattttatg
     6061 cggtgtagca acggggcgca gcccagtaaa gatcaaagaa atcattgatg aattggaatt
     6121 agatatgtat gtcgtgtata atggtcaact ggtttttact gcagatcgca cgattatcga
     6181 ccaccctttt gaacaaaaag tattggaaca tattgttgaa tttgcagatg agaaccatcg
     6241 acaaatcgtt tttggtgcga gaaatcggtt agatggatca acgacgatgc tattaggaca
     6301 atcgattttt atcaagcgac tagtcagctt tttaccgcgt aaattcccgg tacgtctgat
     6361 gaaaaaaatc ctccaggttt ttagccctca ccgacaaaag gatcgttatg aaacgttaga
     6421 gatattaaaa gaaccaattt atcaatgtat attgctcagt ccggagtcag aacagaaaaa
     6481 gttagaacat cgctttcccg agtgtacgtt tcagcgttca aatacgtata ctgtagatat
     6541 catcccaaaa ggtggttcaa aattactagg catccaagct ttcgcaaatg ctgtcgaaat
     6601 tgaaatggaa gaaattatgg cattcggcga tcattacaat gatatcgaga tgctgaaagg
     6661 tgtaggaatt ggagtggcca tggggaatgc tcagatcgaa gtaaaacatg ctgcagactt
     6721 tgtgactcaa tcaaatgaag aagatgggat atacgaagga ctgaaacact tcggtctgat
     6781 ttattagtca atgaaagaag gaaaaaatca gtatggaagc agagacacct tttcaaaaag
     6841 tagaaacatt ccacaaaact tttgatccaa gaagaccaga gatacccacg gcttttacag
     6901 aaaatgaagc tcatacacgt gctgcgttca aaatagaaga acttgttgaa tttgttgcag
     6961 ctacttcacc taataaagaa caactgcatg aagctgttat gctgctgcac gaagcattag
     7021 ataaagcaga agaaaaaata cttggtaaaa cacagtgggg aagtgatccg ctggttgaac
     7081 aagtagatgc actgacagat atcctttact ttacttatgg ttctttttca ttactaggaa
     7141 tagtgcctga tcgaatattc gaaatcgtcc atcaggcaaa tatggggaaa ttatttccag
     7201 atggaaaacc tcattatgac cctgtaacaa ataaagtgtt gaaacctgaa aactggcaga
     7261 tggaatatgc tccagaacct aaaatcgcgg cagaaatcca acgtcagatt caagaagcga
     7321 ttgctaagaa gaaatccgct gatataggca tgtgcacaaa tgaaaaagag tgatcagagt
     7381 ttcactattt ttgttatctt tatacgatgg aaaaacaaaa gattttcgtt taagttagtg
     7441 aatgcatgaa aatagtatgc tataatctag aatagatgtc ttttgcacga gagacgtatt
     7501 atttggattt atgccaagca gaacgccatc tgttgcataa aagatgcata ctgaaaacgg
     7561 aggaaccata aatgggaaat aatgttatca tcggaatcgt cattgcaatc attatagtcg
     7621 ccattgtcgt ctttttggtt agttacttga tgaagaaaaa aaaccaagat cgattggatg
     7681 ccttagaaaa acgcaaggaa gaattatttg acttgccagt gattgaagaa gtcgatgatg
     7741 ttaaaaaaat gcatctggtt gggcaaagcc aaaatacctt tcgtgaatgg agtcaaaaat
     7801 ggaatgacat atctacttcc tcgttcgctg aactagaaag tcaaattttc gaagtggaaa
     7861 acctcaatga aagttttcgg ttcttaaaag gaaaaaaagc agttgttgaa gcagaagcta
     7921 caatggacca aatggagaaa caagtgacgg aaatccgtga aggtctaaag gaattgcggg
     7981 aaacggaaga acgcaactca ctagctgttc agcaggcact tgacgtctat gaagaactga
     8041 aagaaaaagt cagagaagac gagcatgaat acggaccagc tctttttgaa ttgcaaaaac
     8101 agatcaaagt gatcgaaaaa gaatttacgc agtttgtgac tttaaacact tctggagacc
     8161 ctgttgaagc ccgcgaggtt ttggataaag cagaaaaaca tacgtatgaa gtggaagctc
     8221 tgatgaaaaa aattccaccg ctgtatgagg agctccatac cactttccct gaacaacttg
     8281 aagaaatcag tgatacttac gagaaattga tggaagaaca atatgtattc ccagaagaag
     8341 atcttgctga agatatcgca aaagtaagtc gaagaattga aaattcatta gccaatcttg
     8401 aaaaaacaga agttgaaacg gtcgaatttg aaaaccgtga aacagctgat ttgattgatt
     8461 cgttatatga tatcttagaa cgggaaatgg aagctcagag atatgtaaaa actaaccaat
     8521 caacgattgc agagtatatc aaacatacaa caaagaataa ccgtcaatta ttgattgagc
     8581 tggatcacac atctcaaagc tatacattga accacaatga aattggccgt gtacgaggtt
     8641 tccaaaccga agtggaagaa atggaacgtc aaaatgaaca gatgattcca cagatccgtc
     8701 aacacgaaat tccttattca gaagtgcgca cgttctataa aacagtcttc aaagtcttag
     8761 aagatatcga aaagcaacaa gtagagatcg acgactcatt acatgaattg agaaaaggcg
     8821 aaaaagaagc gcaagaaaag atcgatacat tcgaattcaa attgcgcagt ttgaaacgtt
     8881 ttgtagaaaa acaacgtttg cctggcttgc cgaatgatta cctagaattc ttttttgttg
     8941 ctactgaccg tattgaagaa ctaagtgttg tcttgaataa aattcgagta aacatggaag
     9001 aggtcaatcg tttagttgct ctatgtgaag aagatctaga attattagat aagaagacac
     9061 atgatttagt agatgcagca gcattaacgg agcaaatgct ccaatatgca aatcgttatc
     9121 gtcatacaca cgaagaagtg agagaagcaa ttgataaaag ctactattta ttcaataaag
     9181 aatatcacta tcaagaagca ttagacgaaa tcggtacagc attagagcga gtagaacctg
     9241 gtgctttcaa acgcatcgaa gatttttatt ttaatcatcc agacttagta taaagcaaaa
     9301 aagttagtaa accagcaaga gcaagacgga ttattgtctt gttcttgtct ttttttgtat
     9361 atttgaaaat tgaaacaagt gaaaatttca cctgctcttg aaagcacctt ggacaaccgt
     9421 tataatagaa aagaatttca aaaaagaggg gtttcttatg atatattttg ataacagtgc
     9481 aacaacccca atctttccgc aaagcttaga tgcatatgta aaaaccagtc agcggattat
     9541 tgggaatcca tcaagcttgc atgatttagg caatcaagct aaccggttgt tgcagcaagc
     9601 aagaaagcag atcgctgatt tgatccatgt agaaccagaa gaaatctatt ttacttcagg
     9661 cggaacagaa ggagataact gggtattaaa aggaacgatg atcgaaaaaa gagaatatgg
     9721 gaatcatatg atcatttcag gagtcgaaca tccggccgtc agcgagacag cagaacaatt
     9781 aaaagaatta ggaatcgaag taagtatcgc cccagtggat aaacgcggat ttgtacgtgt
     9841 cgacgaattg aaagaattaa taagaaaaga gacagtatta gtctctgtta tggctgtgaa
     9901 taatgaagta ggatcgattc agcccatcca ggaaatcagt gatcttttgg atgattttcc
     9961 taaaattcat ttccatgtag atgctgttca ggcgattgga aaagtaccat tctcgcaatg
    10021 gttgacagaa agagttgatt ttgcaacatt ttcagctcat aaattccatg gcccacgggg
    10081 aactggtttc atttattgga aaaagggtcg tcgtttagca ccattactga caggtggagg
    10141 gcaagaaaga aatcagcgaa gtgggacaga aaatgttcca ggtattgtgg ctatggttaa
    10201 agctttacgt ttatcattag agataaagga acaaaaacct gagcagacac aacgcttaaa
    10261 agtagctcta atcaatgcgt tgaagacgta tgagaaagtg acgatttttt cagaaggaac
    10321 tgactatgca ccgcatattt tatgttttgc tctaaaagga attcgcggcg aagttctcgt
    10381 ccatgcctta gaagaaaaac aaatatttgt ttctactact agtgcctgct cctcacgtaa
    10441 acatatggca agcagtacat tgcacgcaat gcatgtacca aatgatctgg ctacatctgc
    10501 tgttcgaatc agcttagatg aaaacaattc gcttgcagaa gtcgagcaat ttatgattat
    10561 tttcaagcag ctttataaga aattttctgc aatcaattaa aagaggagtg ttcttgtgaa
    10621 atacagtgaa attatggtcc gttatgggga actttcgaca aaaggaaaga atcgaaaaag
    10681 ctttatcatg caactagcac aaaatgtcag acaagctttg aaagactttc cagaaatcaa
    10741 aatccatgct gatcgggacc ggatgcatct tttattgaat ggcgcagacg ggcagctagt
    10801 tattccaaaa ctagcaaaaa tatttgggat ccaaaatttc tctccaagta ttcgtgtaga
    10861 aaaagatgtc gaagttttga aacaagctgt tcaagacatc atgaaagaaa tttatactgg
    10921 gaaagaaaca tttaaaatta tcgctaaaag aagcgatcac caatttgaat tgacaagtaa
    10981 tgaattgaat caaacattag gtaatgctgt ttttgatatt ttcccgcata tccaagttca
    11041 aatgcgtcaa ccagatattc cattacgtgt agagattcgg cgtgatggcg cttatctatc
    11101 gtatgaaacg atcaagggag ctggaggatt gcccgtagga acaagcggaa aaggaatgct
    11161 tatgctttct ggggggattg attctcctgt agcagggtac tttgccatga agcggggagt
    11221 ggaaattgaa gctgttcatt ttgctagtcc gccgtataca agtgaacaag cactgcaaaa
    11281 agcaaaagat ctaacagcca aactagcacc ttatgtggga acgatccatt ttatcgaagt
    11341 gccatttacc gagattcaag aagaaatcaa aaagtcagtg ccacaaggat attggatgac
    11401 aattactcgt cgaatgatgt tgcgtttgac cgatgaaatt cgccgtattc gtcatggttt
    11461 agtcattttg aatggtgaat cattaggtca agtggcttca caaactttgc aaagtatggt
    11521 ggctatcaat gaagtaacga atacgccaat cattcggcct gtagcgacaa tggataaact
    11581 tgaaatcatt gaagtagcag aacagatcga tacatttaat ctttctatcc agccttttga
    11641 agattgctgt acgatttttg ctcctccaca gccaaaaaca cgccctcaac tggaaaaggt
    11701 ccatcaatat gaagagcgtt tagcaataga tgaaatgatc caacgagcac tagctggctt
    11761 gaagatggaa gagatacaag cagaatcgtc taatgaaaaa actgaagaat tttcagaatt
    11821 gctataagag gttatgaaaa tagtgttttg accagagtat tagagaaaca aaacgaaaaa
    11881 atagctccat gtattttacc gtttttgaac taatgtcgaa gattagctga ttgaatacca
    11941 tttatcaaga agttatggaa aagccggttc ttttttacta actgaaaaat aaaattttat
    12001 ttagagatca agctacagtt gataaatgat ataaaaaaga gaatttccca tgaaatagcg
    12061 aggttgtgac agaagtgggc agcttcaaga aataagaagg aatttacgaa aattgctttt
    12121 caaatttttg tgaaatttcg gcttattttt cgaagaagtt acttctggaa caccgtgtat
    12181 cagaggttgc gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagtt
    12241 cctcatattt tacgtttggt tgagcttatt accgcagatg caagcttttg aacaccgttt
    12301 tatttgtctc acaccctttt ccttcctact tgtaaccttg ctcccgtgtt agaaacatgt
    12361 tacactaaat atgtgaaatt attaacaagg agcgtcgtta tgcgagaaaa aacagataca
    12421 ctgccaaaag caactgcgaa aagattacct ttatatttac gctacttgaa gatgctggat
    12481 gactcaggta taagtagaat caaatcaaat gagttcagcg aaatcaccca aattccttct
    12541 gcaacgatcc gcagagactt ctcgcaatta ggcgagctag gcagaagcgg atatggctat
    12601 gatgtccctt ttcttatcga tgtattcaat aatatcctta atactaaaga agaaaaacga
    12661 atcgcattag taggatatgg aaatcttggc aaagcattga aacacaacaa ttttagaaga
    12721 aatgaaaatc taaatatcgt gtgcgtcttt gataatgatc cagcactgat caatcgagta
    12781 attgatggag aaatgatcta tccgattgat cgtttcgcgg agatagccaa agcaaaaaat
    12841 gtgacagtag caatctctac ggtaccaagt aaatattcac aatctgctat tgatgaaatt
    12901 gtcaaaggaa atgtaaccgc tatacttaat tttgcgcctg acagagtaac tgtcccagct
    12961 tatgtcaatg ttcaatacat tgatttgaca accgagttgc agacgcttat ttactttgat
    13021 gagaattact cagaagtttt ttcttaggaa aatttgagta ggcttattga tcttgcgaga
    13081 actcgtcgta attctttcaa aaatttttta gtactaaatg aagtggtttt ttcacgacca
    13141 cttgttgact aaaaaaaaaa accattgtag aatgaagcat aacgctgaac aagaggagta
    13201 actggcacat tcattcttag agagagaact atctgctgaa aggttctcaa tgacaaccag
    13261 tgaaggtagc ttgggagttt ctgtcttgaa tggagtagag atagacgagt cgtgatcgtt
    13321 aactcacttg agaggcaata atgattgatt attgcaattt aggtggtacc gcgtaaatca
    13381 tacgtcctag aatacgaaag tattttagga cgttttttag tttaaaggag gaaaacagca
    13441 tgacggaaga aaataaccta tcaacaaaat ataatccgca agaagttgaa gctggacgtt
    13501 atcagcattg gttagaacaa gatttgttta aaccgagcgg agataaaaaa gcaaaacctt
    13561 attcaatcgt aatcccgccc ccaaatgtta ctggaaaact tcatttaggg catgcttggg
    13621 atacgacctt gcaagatatg ctgattcgtc aaaagagaat gcaaggatat gatactttat
    13681 ggctgcctgg tatggaccat gcaggaatcg cgacacaagc aaaagtagaa gcgaagctta
    13741 gagagcaagg gatttctcgt tatgatctag gtcgtgaaaa gttcatcgat caagtgtggg
    13801 aatggaaaga agagtatgcg agccacatcc gcgaacagtg ggctaaaatg ggcttatctt
    13861 tagattacag tagggaacgt tttacattag atgatggatt atctgaagca gtacgtaaag
    13921 tttttgttac cttatacgaa aaaggtttga tttatcgtgg tgaatatatc atcaactggg
    13981 atcctcaagc acgaacagct ctatctgata tcgaagttat tcataaagat atcgaaggcg
    14041 ccttttatca tatgagctat gaactagcag atggatcagg cgtagtagaa attgcgacaa
    14101 cccgtccaga aacgatgttg ggagatacgg cgattgctgt tcatccagaa gacgagcgtt
    14161 atcaagcgct catcggcaaa aaagtgattc tacctttagt caataaacaa atcccaatca
    14221 ttgcagatga atatgtagat atggaattcg ggacaggtgt agtaaaaatc actccagcac
    14281 atgatccaaa cgactttgag gtaggtaatc gtcatgatct tccacgcgta aatgttatgg
    14341 atgatgatgg tacgatgaat gaactagcag gaaaatatgc tggaatggaa cgttttgctg
    14401 ctcgtaaagc aatcgtgcaa gatctgaaag aaatcggtcg attgattaaa atcgatacga
    14461 tgatccacag tgtgggtcat tccgagcgga caggtgtcgt tgttgaacct cggctgtcta
    14521 ctcaatggtt tgtaaaaatg gcgccgttag cgaaaaaagc cattgataac caagaaacag
    14581 atcaagcagt ggaattcttc cctccaagat tcaatcaaac gtttttacgt tggatggaaa
    14641 atgtccatga ttgggtaatc tcacgacaat tatggtgggg acatcaaatt cctgcatggt
    14701 atcacaaaga aacaggtgaa atgtatgtag ggatggaagc accagcggat agtgaaaact
    14761 gggtacaaga tcctgatgtt ttagacactt ggtttagttc agctttgtgg cctttctcaa
    14821 caatgggctg gccagatacg gaaagcgaag attataagcg atacttccct actagcactt
    14881 tagtaaccgg ttatgacatc attttcttct gggtaagcag aatgattttc caaagcttgg
    14941 aatttactgg cgaacgtcca tttgagaatg tcttgattca tggattgatt cgtgacgaac
    15001 aagggcggaa gatgagtaaa tcattaggta atggaatcga tccgatggaa gtaatcgaaa
    15061 agtatggagc agatgctctc cgctggttct tatcgaatgg ttctgcacca ggacaagatg
    15121 tccgcttcag ctatgaaaaa atggatgctt cttggaactt tatcaacaaa atctggaatg
    15181 cttcacgttt cgttatcatg aatatcgaag gaatgaccgt agaagagatc gatttatctg
    15241 gagaaaaatc agtggctgac cggtggatcc ttactcgttt gaatgaaacg atcgaacgag
    15301 taactgagct atttgatcgt tttgaatttg gtgaagcagg tcgacaactg tataacttta
    15361 tctgggatga tttttgtgac tggtacatcg aaatgagtaa agaagtactt tatggagatg
    15421 atgctgtttc aaaacaaacc acccgtagca tcttagtcca tacacttgat cagatcctac
    15481 gtttattgca tccaatcatg ccattcgtta cagaagaaat atgggaacat attcctcatc
    15541 agggaaattc tctagtagtg gcagaatatc ctgttgtacg tccagagttt gatgatgaaa
    15601 cagcttctaa agggatggaa gtattgaaag aattgattcg agccgttcga aatattcgtt
    15661 cagaagtgaa tacaccattg tctaaaccaa ttacgttgct aattaaaaca aacgatgcta
    15721 aaatcgatca atttttagtt gaaaatacaa gctatatcga acgcttctgt aatcccgaag
    15781 agttgacgat atcaagtgag atcactgcgc cagatctagc gatgtcagct gttctgacag
    15841 gtgcagaaat cttcttgccg ttagcaggat tgatcaatat tgaagaagaa atcaaacgat
    15901 tagaaaaaga actggaaaag tggacaagcg aagtaaaacg tgtccaagga aaactttcta
    15961 atgaacgttt tgtttctaac gcgccggaag aagtagtaga ggcagagcgt gcaaaagaaa
    16021 aagattattt agagaagcaa acggctgtca aagaacgaat cgaaagtttg cgtactattc
    16081 aataagaata gaaaaagaga gtgtgaaatc agctagctat tactaaaagc tagctgattg
    16141 aacatcgtta caggaaagag tttgagcctc actcttagct tcgattcata aggagatgtg
    16201 acataaattt tgtcgcatct cttttttagt ctactcttat tatgtaggag aaggagggag
    16261 gtggatcatg tatcaagagt caataaagta gttgataaaa tcgttcctct ctctgtttct
    16321 tttctctttg tcatactttt atttttctta tgtgttctat ttgttataat aaaagagaaa
    16381 tgggggaatt gttgatgacg attgaagaag caatagaatg gatccatagt cgattaccat
    16441 ttggttcacg tcctggcctt gaccgagtcg aagctttatt agaagtggta gggcatccag
    16501 agagaaaagt aaaaacgatc catatcgctg gaacaaacgg aaaaggttct actgtagctt
    16561 atttgagaag cctattagaa gaggctgggc tgacagtagg tacatttact tcaccttata
    16621 tcgaaacgtt caatgaacgg atcgcaatca atggcaaaag cattccagac aatgatttga
    16681 tccagcttgt aaaaaaatat cagccactga tcaaacaact tgatcaattt gaagcagtag
    16741 ctggtattac tgaattcgag acattaacag caatggcatt tgattatttt ttgatgaaga
    16801 aagtggacgt agcgattatc gaagttggct taggcggctt attggattct accaatgtag
    16861 ctgtaccaat gctgacggga ataacaacga ttggacttga tcataccgaa atacttggag
    16921 atacaattga agaaatcgct gcacaaaaag ctggaatcat caaacaaggc gttccagtag
    16981 taacaggaaa tattgcccca gaagctttta ctgtcatcca acaaacagct aaagcaaaaa
    17041 atgcaccgct ttatgtatac ggggaaaatt ataaaataaa ctatctgcat cctgatgaga
    17101 tgtggggaga agtatttgaa ttttttaatg aaataggaaa gatccctcaa ttaaccactc
    17161 cgcttttagg gcaacatcaa gctgaaaatg caggagtagc catcgaattg tattttgttt
    17221 actgtcaatt ggaaaagctg cctttccgag aaaaagatgt tttgaaggga ttgaagaaat
    17281 ctcgatggcc aggaagaatg gagcgtttga gtaacgagcc attagttgtt ttagatggtg
    17341 cccataatac gcatgcaatg aaccggcttg ttgagaattt gaaaaaagaa ttcaaaggat
    17401 atcaaatcaa cattttgttt tccgccttac gaactaaaaa tgttagagaa atgctagaac
    17461 gattgcagca agtaccaaat gtccatattt atttgacgac ctttgaacat ccaaaagcca
    17521 tcgaattaag tgaagtatca gatttagcaa gtgagagagt atcaatcgtt tcattgtggc
    17581 aattcggatt agcagatata ttggaaaaga tgacaaatga agacgtatta gttgtgaccg
    17641 gttcacttta tttcatttca gaagttcgcc atttattaat acaattagga ggagatcatg
    17701 aagtataatg cagtgatttt tgacatggat ggtttgctgt tcgacacaga gttagtgtat
    17761 tacgaagcca cacaagtagt agcagacaag atgggttttc cttatgataa agacctgtat
    17821 ttaaaattct taggtgtttc agatgaagaa gtatgggcga attatcatat gattttctcg
    17881 gaatttggca aaacaagtgt acaaagattc atagatgatt cataccagga aacgcttgaa
    17941 cgtttttcgc aaggagcggt acaaatgaag cccggtgtgt tggaactcct ctctttttta
    18001 gaagaacatc agattcccaa agttgtcgct tccagtaacc aacgagcagt cattgaactt
    18061 ttattgaaga agaacaatct gtttgaacga tttgagacga ttgtttctgc agaaaatgtt
    18121 caacgagcaa aaccagaccc agaaattttc ttgttagcta atcagaagct aggtacagcg
    18181 aagaaagaaa cattgatttt agaagattca caaaatggta ttttagcagc ttctgcggct
    18241 gagatcccgg tagtcatggt accagatctg cttccaccat cccaagaatt gatcaaaaaa
    18301 acagctgggg ttgtttcttc tttacatgaa atccctgcat tgttaaaata aaaatccatc
    18361 tcttttcgta tcttatttta gataagatag aaaggagatg tttttttggg aaaacgattg
    18421 atcaaagaag tacctactag ctctttacca agggaacgta tggaaattta tggcgcggaa
    18481 gctttatcgg atcaggagct tttggcaatc ttactgcgta ccggtcaaca cccttatagt
    18541 gtgatgtcga ttgctggaaa tctgttaaaa acgtttggag gactggcctc tttacgacag
    18601 gctactttgc atgagttaga agagattcga gggataggac gcgtgaaagc aatcgaaatc
    18661 aaagccttga tcgaattagg tcgacgaatt cagacggatc atgaaagagc ggctccaccg
    18721 gttcgtacca gctacgaatt ggcccagcaa ttgattctgg aaatgaaaga tcacaaacaa
    18781 gaacatttgg tttgtattta tttggacacg aagaatcaag tgatcttaaa aaaaacagtg
    18841 ttcatcggtt cgctgaatca gtcagtcgct catcctagag aaattttcca ttatgctgta
    18901 cgttattgtg cagcaaggat cgttttagcg cataatcatc cttctggtaa tgttttaacc
    18961 atagtataat aaataatagt agttaaagta gtttaagagg agatattaaa gggatatttt
    19021 actttaaata aaaattaaca aggtttataa tttgtagtag ttaaagtatt taaagaggtg
    19081 ggaacatgtg aaggttcaaa ggatagaagt ggagaataag ccgtatccat tgtatttatt
    19141 actagataaa gaataccagc taatagaacc agtaatgaaa tttattaaat acttagataa
    19201 tactggtaag tctcctaata ccattaaagc atactgctat catttaaagt tgctgtacga
    19261 gttcatggaa cagagaggtg ttattcttaa tgatattaac tttgagttgt tagcagactt
    19321 cgtaggttgg ttgagatatc cttcagcatc aaatgtaatt gatcttcagt caaaaaaagc
    19381 cataagagaa gaaacgacag tgaatacaat tttaaatgta gttatgagtt ttcttgatta
    19441 tttaagtaga ttaggagaat ttaaatcaat tgatgtattt aaacaagcaa agggaagaaa
    19501 tttcaaagga tttttacatc atgttaataa gggtagatac caaaagaatg tcttaaagtt
    19561 aagggttaaa aagaaacaga taagaacatt gagatcagag gaagttaagc aaattattga
    19621 tgcttgtcat acgaaaagag ataaattaat tttaatgctt atgtatgagg gtggtttaag
    19681 aatcggtgaa gtgttatcgc ttaggcttga agatattgtc acttgggaca atcaaatcca
    19741 tttaacacct agagatgtta atgttaatga agcttatatt aaattaagga aggaaagaac
    19801 aatacatgtg agtaaagaac ttatgtcact ttatacagat tacttgatat atgagtatag
    19861 tgaggaattg gagcatgatt atgtttttat ttccttaaaa gaaggctatt ttgggaaacc
    19921 actaaagtac caaagtgttc ttgatctagt tagaagaata gttaaaagga ctggaataga
    19981 atttacatca catatgcttc gccacactca cgcaacgcag ctaattaggg aaggatggga
    20041 tgttgcgttc gttcaaaaga gattaggtca cgcacatgtt cagacaacgt taaataccta
    20101 tgttcatctt tcagatcagg atatgaaaaa tgagtttaat aaatacctcg agagaaagga
    20161 gcataagaaa tgaatgcttc tagtaaaagg aaaattatta gtcagagtga gattagcaaa
    20221 aaaatagctg taatgaatga agaaatgcag gggttttggg ctaataatag ttgggatata
    20281 agaaaatgtc cacatccttc tgccatagaa ttaagtaaga atcctgcttt aaggaatcgt
    20341 tgggttcgtt ttgaacgtgt taaaaatctg tggttaagaa cagaattgaa atatttttat
    20401 ttttaccatt taaacaatgg aatatggaat gcaaaaactg tctggattag aaaaggaaca
    20461 gtaattaata aaatgttaga tttcttagat ttaaagtatc ctagcattac ttcaattact
    20521 gaagttccta ttgaaaaagc aatgacggag tatagaactt atttgacaaa acggggtgtt
    20581 agaattacca ctactaatta taagattact gctaatcaag aaaaaacacc tgtaaaagct
    20641 aattcctact atgttactaa tctaaaacaa tttatggagt tttatgagaa cttttatttt
    20701 gatggagagg agtgggataa agacgtttgg gatagacgta acttaccttt gccagatgat
    20761 aaggttaacc caacacaata tgaatataca attaacttta aagggtttcg gaatacatat
    20821 tttaaacaac ttgtaaaaag atattgtaag ttgagattga acatggatag cttttcctat
    20881 gtaagtgata ttgcccaaag acttaaagag ttctttaatt ttctggacat gaaatttaaa
    20941 caagttcaga gagtacacca attaacgaga gtggaaattg aagcatattt aagtgaacta
    21001 aacatgatgg gaataaaacc tagtacaata actgggagga tctctatatt ggaaggacta
    21061 tttagtaccc ttcttaggct agaatgggat gatgttcctt ccaaaatatt aatttattct
    21121 gaggactatc cgaaaatacc aagagcaaaa ccacgcttta tagatgaatt cgtcctagag
    21181 caattgaaca gtcatcttga taaattaccc gaatatatag ctacgatgac tatgattgtt
    21241 caagaatgtg gaatgaggat aagtgaattg tgcaccttga aaaaaggctg tctattagag
    21301 gacaaagatg gagatttctt tttaaagtat tatcaatgga aaatgaaaaa ggagcatata
    21361 gttccaatat ctaaagaggt agctttactt attaaagttc gggaagataa agtttcagag
    21421 gaatttccag atagtgaata cctctttcca agaaaagatg gatcgccatt aaaacaagaa
    21481 acatttagag gtgagttaaa taaattagct tatgagcaaa atatagtgga taaatcaggt
    21541 gagatttata gattccatgc ccatgccttt cgccatacag taggaacaag aatgattaac
    21601 aacgggatgc cccagcatat tgtgcagaaa tttttggggc atgaaagccc agaaatgaca
    21661 agcagatacg ctcatatctt tgatgaaact ctaaaaaatg aatttactaa atttcaggaa
    21721 aaactggtta ccaataatgg agatgtgctt gatctagatg aagataatga agtcgatgat
    21781 gtagagcttc aatggttcaa gaaaaatata aatgcacaag tgcttccaaa tggttattgt
    21841 agattgccag tagtagcagg tggttgtcca catgcgaatg catgcttaga ttgcactcac
    21901 ttctgtacca gtaagcaatt cttaccacag cacgaagaac agttagagcg tacagaagag
    21961 ttattagcca tagctaagga taaacaatgg caaagacaag tagagactaa tagccgtgtt
    22021 aaagagcgtt tagaacaaat cattggaagt ttgacggggt aattatcaat ggataaacaa
    22081 gttagaaata caacagaaat tgtacgtttg gcgaagcaga aatcaaaaaa gacaagggaa
    22141 aaagtagaca aagcgatttc taaattttcg attgaaggta aagttattaa ttttaattca
    22201 atagcaaagg aagctaatgt ttctaaatca tggctttata aggaacacga tattaggcaa
    22261 agaatcgaat cccttcgtga gcgtcaaata acagcaaatg tagtctcaaa acccaagaaa
    22321 agttctcgtt cggaggaaat ccttattaaa accttaaaaa gaagagtaat ggaattagaa
    22381 aaagaaaata aaaaattaca gaaccaaatt caaaaattat atggagatct gtataataaa
    22441 gaataattat taatctgtag acaaattgtg aaaggatgta cttaaacgct aacggtcagc
    22501 tttattgaac agtcatttag gtatatgtcc aatctagggt aagtaaattg agtatcaata
    22561 taaactttat atgaacataa tcagttacga ggtgaaatca tgagcaattt gattaacgga
    22621 aaaataccaa atcaagcgat tcaaacatta aaaatcgtaa aagatttatt tggaagttca
    22681 atagttggag tatatctatt tggttcagca gtaaatggtg gtttacgcat taacagcgat
    22741 gtagatgttc tagtcgtcgt gaatcatagt ttacctcaat taactcgaaa aaaactaaca
    22801 gaaagactaa tgactatatc aggaaagatt ggaaatacgg attctgttag accacttgaa
    22861 gttacggtta taaataggag tgaagttgtc ccttggcaat atcctccaaa aagagaattt
    22921 atatacggtg agtggctcag gggtgaattt gagaatggac aaattcagga accaagctat
    22981 gatcctgatt tggctattgt tttagcacaa gcaagaaaga atagtatttc tctatttggt
    23041 cctgattctt caagtatact tgtctccgta cctttgacag atattcgaag agcaattaag
    23101 gattctttgc cagaactaat tgaggggata aaaggtgatg agcgtaatgt aattttaacc
    23161 ctagctcgaa tgtggcaaac agtgactact ggtgaaatta cctcgaaaga tgtcgctgca
    23221 gaatgggcta tacctctttt acctaaagag catgtaactt tactggatat agctagaaaa
    23281 ggctatcggg gagagtgtga tgataagtgg gaaggactat attcaaaggt gaaagcactc
    23341 gttaagtata tgaaaaattc tatagaaact tctctcaatt aggctaattt tattgcaata
    23401 acaggtgctt acttttaaaa ctactgattt attgataaat attgaacaat ttttgggaag
    23461 aataaagcgt cctcttgtga aattagagaa cgctttatta ctttaattta gtgaaacaat
    23521 ttgtaactat tgaaaataga aagaaattgt tccttcgata gtttattaat attagtgaca
    23581 tttgcatgct tcaaagcctg tcggaattgg tttttagtga aaagaacacg atattcacgg
    23641 tttacccact tataaacaaa agatcgatac tttttgtagt ccttctttga aatcaatggt
    23701 tgatgtcgtt caagaacaat caatacagag tctacacttg gcttaggatg aaaatatagt
    23761 ggtggtactt ttttgagcat ttttatatcc atctccacca ttaatagtaa acccaaagct
    23821 cgttgcagat tttgcaatct tttcgcaaat cccttctcaa cgataagata gctatattta
    23881 gcctgacttt caaaggtaat tcttttgaca atatccgtac tgatgttata aggaatatta
    23941 ccatatatct tatagtttat atgttttggg aaggaaaatt ttagaatatc cgtttgaatc
    24001 acttttatat tctcagaggg gtttaccgct tctttagtca cttgacataa gcctccatca
    24061 atttctatag cagtaactga tcgactcatt ttgactagct ctttggtaaa atgtcctttt
    24121 cctgatccga tttctattac gttgtcttgt ttactgatat tcgtgtgatt caatatttct
    24181 tttacatgct ttttagaagt aataaaattt tgcgtgtctt tagggttttt ctggttcatt
    24241 ataaccttct ccttactggt tataatgaac tgacattaag cgttcattat aaccaattaa
    24301 ttttgatttg gttgataacg aactttatta ataacaaata tagaaaaagt acccatactt
    24361 ttatctcctt tccatttttt tcattatgaa tgagataaag taatttctac tactgcgata
    24421 ctggtgcaca taattgaaac ctccttatgt aactgaatat gattgtatca tcttttcatt
    24481 ttagaataaa cctttatatg taattttgca cttgacactt cagatggacg ttttcttcaa
    24541 aataatatgt tttgaaactt gataagagaa gccactgtct ttactaaaaa gtaaaggctg
    24601 cgataaagga cagtgacttt aataaagttg cactgttgta ttgtctttgg ataaaattgt
    24661 ctaaagggaa gttttatccc caacgaaaaa acatctaaag tggtaagtaa tgaaagaatg
    24721 tcgataaagt aggaatgggg gatctttttg aatacaatag aatcacagtt tgataaggtt
    24781 gcagaagatt acgatttcgt gaatgagctt ttgaatgatt attcattctt tgtgtctaat
    24841 atgtctccaa agaaaggcag agcattagat atcggatgtg gctcgggttt gttagtggag
    24901 aaattagcaa gttattatga tgaagtggta gggattgata tttctaatca aatgctcgat
    24961 cttgccaaat ctaaacgtca actaacaaat acggtctatc tgaatatgaa tgcagaacaa
    25021 cttaatttta atgagaagtt tgattttatt gtaagccgaa caacctttca tcatttggat
    25081 gatatagcca gcgtaataca gcaaatgaag gaactgttga atgaggaagg aagaatagtt
    25141 attcttgata atgtatctga agtcgagaca ccgcctacct atgtttataa attaggggca
    25201 atccaagagt ttttaccgca ttgtttcaaa tttggaataa aaaatgcgat tagaatttat
    25261 aatcacaaca catctaaatc atggcttgag catttagctt cagataagta cctatctgaa
    25321 caaaattatt acgatttata tgaaaagttg ttgcctggat gccaattcca taaaatgggt
    25381 tgggcaatgg gggttgtctg gacaaaataa cttaaagctg tggctttgaa aagttgaatt
    25441 tgtgattgtt ttcagagcta tcgggtgctt tagtataaat aacaatgaat aaaaaatcag
    25501 cctgtatctt atgttactat taagtaattt aagatacagg ttttactatt tttgaaataa
    25561 gtaccttcca cttcaaatac atgtattcaa agtgaaagta ttgatttaaa tgattctttt
    25621 gtaatttact cttgataata tgaaattcca tcgcagcagg atatgaattt taccaaaagg
    25681 atacaaaagt gtggagaaat gatggggatc actgtactgg accacttgat catcggacgc
    25741 aaacgatatt ttagtttgag agaagaagga atgatggaag agaagtaagt tgctttcttg
    25801 caaaaaaaat gaagcaagtg tacaattata ttgtaatttt gtttgggaat agaagttgaa
    25861 acatctgttg cacctctatc gtacacgagc aatgttacaa tttaaattag tgcttttggc
    25921 acgaggagga tattattatg gaaaacggaa cagtaaaatg gttcaacgct gaaaaaggat
    25981 ttggatttat ctcacgtgaa gatggaagcg acgtattcgt tcacttctca gctatccaag
    26041 gtgacggatt caaaacatta gaagaaggtc aagcagtgac tttcgacgtt gaagaatcag
    26101 accgcggccc acaagctgca aacgttgtaa aagcttaatt ttatctgtaa atcaaattga
    26161 tggtagacat caatttttac gagagaacaa agcgagtaac aaaaatacct gccacaagag
    26221 acaatttctc ttgtggcagg tatttttttg tatgaaaaag taaataaatt taagaggcta
    26281 tgaaagagcc accttgtatc aaggaataag aacaaacaaa aatggcttct catgtttttc
    26341 atttgttttc tcttatattc aggacaaggt gctgatttca taacctctta tttattcttc
    26401 atctggtgtc attaccattg gcaaaatcat tggatgtctt tctgtacgtt cgaataagaa
    26461 aggctgaagc gcgccagtca tcgcttcttt caatttatat tctgaacagt caggtccatt
    26521 caaacattct cttaaagcgt ggaataagat tcgctgagct tcattgatca tttctcctga
    26581 ttctcgcatg tagacaaatc cacgagagag aatatctgga ccagccaaga tttctttttt
    26641 cttgatatcc actgtcgcga cagctaatac aagaccttct tctgaaagca ggtgacggtc
    26701 acgtaaaacg acattaccaa tatcaccgat cccgctacca tcgatataca catctccagc
    26761 cacaaaatgc ccagcaggac gcgcagaatc gctagttaac gctaaaacat cgccattttc
    26821 catgatgaag cagttttctg caggaacacc tacatcttgt gctagagaag catgtacttt
    26881 caacatgcgg aattctccgt ggacaggaat aaaatatttt ggcttcatca aacgaagcat
    26941 taatttttgt tcttcttgtc caccgtgacc ggaagtatgg atgttattga tttttccgtg
    27001 aatcacttct gcaccagctt cagacagcaa attgatcaaa tgattgacac ttgttgtatt
    27061 accaggaata ggtgaacttg aaaagatcac tgtatcgtct ggctggattt ggatttgacg
    27121 gtgagtacca ttcgcgatgc ggctcaacgc ggccatcgct tctccttgag aacctgtaca
    27181 taggatcatc gtttcattgg caggcaagcg attgatttcg tgtgcatcta caaatgtgcc
    27241 gtctggaact ttgatatatc ctaaacgttg accgttgacg atggcatttt ccatgcttcg
    27301 tccgaaaaca gcgattttac gaccagttgc cacagcggca tctgctgctt gctgcaaacg
    27361 gaagatgtta gaagcaaagc tggcaaaaat gatacgtcct tcgatttttg aaaagatctt
    27421 caaaatagaa tgcccaatcg ttttttctga tttagtaaat gttggaatct cagcatttgt
    27481 actatcagat aaaaggcaca agactccttc ttccccgata cgagccattt tatgcaagtt
    27541 tgctggttcg ccaacaggag tgaaatcgaa tttgaagtct ccagtcgcta cgatatttcc
    27601 agaaggtgtt ttcaccacga tccctaatgc atctgggata ctatgggtcg ttctgaaaaa
    27661 gctgacagaa gtctttctaa aacggatgat cgtatcttca ttgatctcat ggagctctgc
    27721 atctcgaagc aagccatgct catctaattt gtttgtgatc aacgctaatg cgagaggacc
    27781 agcatagata ggaatatttg cttgtttcag caagtagggt attccgccga tgtggtcttc
    27841 gtgcccgtga gtaatcacca aggcttttac tttgtgtaag ttttgtatga tgtagctgta
    27901 gtcgggaatg acatagtcaa ttccaagtaa atcgtcttcc gggaatttga tcccggcatc
    27961 aatcaaaatg atttcatctt ggaattgtac cccatacgta tttttcccga tttcacctaa
    28021 tccaccaata gcaaagacgc ctgtctcgtt atttttgatg gaaagtttca tattaaaact
    28081 ccactaatga gaaatcagca ttttcttttt cgtactctag atgacttcct tctagaagtt
    28141 gaacgaattc aatattgtat ggggtatttt cttccacacg ttggcgagcg tctacatcgc
    28201 tttttgcctc aatgtaaaga gattttgttt cttcccgttt agggttgcgt acttttgttt
    28261 cttgataata aactttatag atcattgttt tgtctccttt atctgcgtaa agcagtgatt
    28321 tagctagttg atttttttag acaacacaaa acgaggggac aagccacctg gtttttcata
    28381 tgctttgact agaatactgg attcgagtca aagagacaag cctcgttttt gcttatctgt
    28441 tcggatattt tttcaaccgt accttctgta taaaaaatat acaacaatta attgttattc
    28501 tagcataaaa cagcaaataa gtatagaaat caactagatt gaaagaaaaa atcaatgttt
    28561 atcatcccgc tataatcata atgacttttt tatgagagaa gtgttctatt aataggctcg
    28621 ttgtgctatc atttttagta atgaaaatgt aacaaaagat taaaaggaga tgagaaagtg
    28681 aaactaatgt ggcattatac catgcgttat aagaaattct tgtttttcaa tttcatatgc
    28741 gtatttggtt ttattttgat tgaattagga cttcctacga ttttagcacg tatgatcgat
    28801 gtaggaatcc gcaatgatga ttttgattat gtgaagcaac aagggatatt tatgatcgtt
    28861 attactgtaa tcggtattat tttgaatatc ttgttaggtt attttggttc gaggattacg
    28921 actaatatcg tagcagatat tcgagatgat ttgttcgaaa agatccaaag ttattcgcat
    28981 caggaatacg aaaatctagg cgtatcttca ttgatcactc gtacaacaaa cgatgcttat
    29041 cagatcatgt tgttcctgca aaatatttta cgtatcggtt ttatggcacc aatgatgttc
    29101 attgtcagtt tgtacatggt catgcgtaca agtgtgacac tcagtttgta tgtagtaggt
    29161 gcattacctt tactattatt ggcagtcgta gcgattgcta aggtgtctga accgttatct
    29221 aaaaagcaac aaaaaaactt agataaaatc aacagtatct tgcgggaaaa tctttcagga
    29281 ttgcgtgtga ttcgtgcatt cgtcaatgaa aagtttgaag aaaaacgttt tgctgtagtc
    29341 aatgagaatt atgcttctag ttcaaaaagc ttattccgtt tgatggctgt tgcacaaccg
    29401 ggtttttatt tcttatttaa tatcgtaatg gtattgatca tctggagcgg ggcagtgcaa
    29461 atcgatgcag gcgagcttcg tgtaggagat ttgattgcgt ttatcgagta tatattccat
    29521 gccttgttct cattcatgtt atttgctagt gtgttcatga tgtatccaag agcagctgtt
    29581 tcagctggac gtatccaaga agccctagat gccaaacctt cgattgtaga agatccaaat
    29641 ggtatatctg atacaaaaac taaagggtat atcgaatttc aaaatgtaac atttgcttat
    29701 ccaggccatt cgcaagagcc agtgatacga aatgtcagtt ttacagcctc tccaggagag
    29761 accgttgctt ttattggaag taccggtagc ggaaaatcta cgttgatcca attgatccct
    29821 cgcttttatg atgtgagtga aggaaagatc ttgatcgatg gagtagacgt gagagattat
    29881 cggctaagca agttgcgaga taagattggg tttattccgc aaaaagcgct gctatttact
    29941 ggaacgattg cagataattt gcgatacggg aaagaagatg ctacgcaaga agaaatggaa
    30001 cgtgcagctg agattgctca agcttcagaa ttcatttctc gaaaaccaga tggatttaat
    30061 gaacatttat cagaaggtgg ttccaatttt tcaggtggac aaaaacaacg attggcaata
    30121 gccagagcca tcatacgccg tccggaaatc tatatttttg atgacagctt ttctgcatta
    30181 gattatcaaa cggatgcgaa gttacgtgct cgtctgaaaa aagaaacgac agaatctact
    30241 gtattgatcg tagcacaacg tgtgggaacg atcatgcatg cagataagat catcgtatta
    30301 aatgaaggag aagtcgtcgg tatggggacg catcgggagt tgttggaaaa atgcccgatt
    30361 tattatgata ttgccgcttc tcaattatca aaggaggaat tagcatgaaa aaaacctttt
    30421 cttccttaaa acgtttagct cgatatatca gaccgtatcg tgagactttt cttttagtga
    30481 tcctatttac agttctgaca gtggctttca acgtggcttt gccatatgtt acaggacttc
    30541 ctactacaga aatcagccgt aatattgcca atggcgaatc aatcaatttt tcttatatat
    30601 tcaaatgtct catttggatc acagttgttg gaatagggta ttgtatttcg caattacttt
    30661 ctggtgtttt gatgacaaat gtcgttcaaa gtgccatgca tgatttgaga agagacatag
    30721 aagaaaaaat caatcgtctt ccggtttcat atttcgataa aaaccagcaa ggaaatattt
    30781 tgtctcgtgt gacgaatgat gtagatgcag tcagcggtgc tttacaacaa gcttttattg
    30841 gtatcgtaaa tgcgatattg ggaattacgt tggctgcagc aatgatgttt tatatccagc
    30901 cgatgatggc gctgatctct atgatcatga ttccaacatc gatctggatc tcaaaacgag
    30961 tgatcaatgc ttcgcagaaa tattttcaaa gcatgcagaa ttctcttggt gagctaaacg
    31021 gctacgtcca agaaaatatg acaggtttta gtgtattgaa agtatatgga cgcgaaaaag
    31081 aaacattgga aggattcaaa aaagttaatc acagcttgaa atactttggg tttcgtgcag
    31141 cattcatttc tggcttgatg atgcctctag ttcaattaac tgcctatgct acttatattg
    31201 gtatggctgt tctaggaagc ttttatgtta ttactggggc gatcgttgtc ggtcaactac
    31261 aagcattcat ccaatatatt tggcaaatca gtcagccaat ggggaacgtg acacaattgt
    31321 cttcagtttt gcaaagtgca tcagcggcaa cgaaacgtgt attcgagatt ttagatgagc
    31381 cagaagaaaa agaaaacaaa gtagatgctt cactgccaga aacgatttta ggagatgtga
    31441 cattcgatca tgtcgatttt gcatatgatc cgaagaaacc attaatccaa gatttgaatt
    31501 ttgaagtgaa agctggtcaa accgtagctg tggtcggtcc gaccggtgcc ggcaagacga
    31561 cattgatcaa tctattgatg cgtttttatg acgtagacaa aggagcaata aggattgatg
    31621 gaatcgacac caaatcaatg agtcgcagtg atgtgcgttc gctatttggt atggtcttgc
    31681 aagatgcatg gctttatcaa ggaacgattg cagataatat ccgcttcgga aagcttgatg
    31741 cgacagatta tgaagtagtt gatgctgcga aaacagcaaa cgtagatcat tttatccgaa
    31801 cgatgccaga tggatatgat atggccatca attctgaggg agacaatgtg tctttaggac
    31861 aaaaacagtt gctgacgatt gcccgagcag tggtctccga tcctaaaatc ttgattttag
    31921 atgaagcaac aagttcagta gacacccgtt tagaagcgtt gattcaaaaa gcaatggatc
    31981 gcgtgatgga gggacggaca agtttcgtca tcgctcaccg tttatcgaca atccgtgatg
    32041 cagatctgat tctagtgatg gatcaaggtc agatcattga aaaaggaacg catgagagtt
    32101 tattggcaca aggtggattt tatgaaaaac tgtataacag ccaatttgct gaggaagcag
    32161 aataagtcat ataagaataa aacagtttaa aacttatttt ttgttctcta ttcttgataa
    32221 tggtaaagta aagttatcaa gaaaagtgag gacacggaga tggaacagtt tttaaatgaa
    32281 aaaattactg aagccaaaag agaagttgat cgtttaacgc acgtctgtca gacgaagcct
    32341 tttgattcag ctgatttttt tgaaaatgag tttcaaatcg aacgcttggc agcacaaata
    32401 gaagtgtacg aattaatttt aaaaaaagta atagaagaaa atatagatgt acaataaaaa
    32461 caggtaaaac gtcagttacc agatactgta aatagacatc tggcctgagc aggttttacc
    32521 tgtttttttt cttatgaaat aaatatatgc tatgatacct ttattcaata ggattagttt
    32581 ctacaaaaaa tgaaaggaga aaaataaata tggaacggtt tatagcatca aatgccacag
    32641 tgattggtga tgtgacattg tcagaagatg tgacgatctg gtatcaagct gttttacgcg
    32701 gggacagtaa ttggataaaa ataggacaaa gaacgaatat ccaagatggt acaatcatcc
    32761 atgtcgatca tgatgcacct gtagatattg cggagaatgt gacggtagga catcagtgca
    32821 tgctacatgg ctgtacaatc gaaaaagggg cgctgatcgg gatggggacc acgatattga
    32881 atcatgcggt tattggtgag aatagtttga ttggtgctgg atcattagta acagaaggaa
    32941 aagttattcc tcctaatgtc ttggcttttg gtcgcccagc taaagtgatc cgtcctttga
    33001 caaaagaaga aatacaaaaa aacaaagaaa acatccagca ttatgtcgag ataggtcgga
    33061 tgcatgcgca aggaaaattc aaggagcaaa gataggcagt agcatccata aaataaaaaa
    33121 ggatggtcag tttgagttga ccatcctttt ttatttggtt tagataagag agcttaaaaa
    33181 agcttccttt gaaacagatt aatttaatga agaactgttt tccatgactt catcgatcaa
    33241 tccatatgct tttgcttgtt cagctgtcat atagttgtca cgatctgtat ctttttcaat
    33301 cacttcaagc ggttgtccag tgcgttcagc taaaattttg tttaagcgtt cgcgtgtttg
    33361 caaaatatgg cgtgccgcaa tttcgatttc agttgcttgc ccttgagcgc cgccaagagg
    33421 ttgatggatc atgatctcag catttggtaa agcaaatcgt ttaccttttg taccagctgt
    33481 cagcaagaag cttcccattg aagcagccat acccataaca attgtttgga catctgcttt
    33541 gacaaagttc attgtgtcgt aaatagccat acctgctgta actgaaccac caggagaatt
    33601 gatataaaga tagatatctt tttcagaatc ttgagcgtct aagaaaagca attgggcgat
    33661 gatcgaatta gcaagatcat ctgttacttg gccgcttaac atgatgatac ggtctttcaa
    33721 taaacgtgag tagatatcgt aagctctttc acctcgtgaa gattgttcaa tgactgtagg
    33781 aattaaattc ataatagagt tcctccttga tttcgtttag aaatagtgta ccataattta
    33841 gtttctaata cctaaaatgt agtatacatt tttggtcaaa aaaggtcaaa taaaaaactt
    33901 aggcaaatta gtcgcataaa cccgctttat ggtatgataa gcagagaaac aaaacaagga
    33961 ggaacaacag attgatagtt ggtattactg gccttatcgg agtgctgaca ggtgctgtag
    34021 ttgtctatat cggcatgata ctgcaaatgc gtcacttgga aaataaagaa cagcgtagac
    34081 gtgaattgga attgaaagtc aaagaaatcg agacgctgaa tcaattaaat aaaaagatca
    34141 acgagatctt gcaaaaacgg acaacgctga tggaagaata tgtcagcttt gatgcatttg
    34201 atgattgtta tatcacgatt gacgattttg cgtatctcca atcattttca gcgcaaaata
    34261 gtttttatct gccaaattat attttagacg agctatttaa gaatatctcc catcgaaaag
    34321 tgattttatc tccagatgaa acaatgaaga ttggtggata tgcttataaa ggcggacgcg
    34381 tgattttaga gaattttacg gatagtctga ctgaaatgat caatgaaaaa aaagctcagt
    34441 tgaagactat gacgcgccag ccgattggtt tcttctcgaa agatgatgtt tggagctgat
    34501 attttgaagc attgccgttg cacatgagag cgtggtaatg ctttcttttt ttcttaatga
    34561 tacaatagaa ctgaacggag tgaagaaaga tgttacaaat ctatgcccat cgcggaagca
    34621 gcggcaccca cccagaaaat acgcttcctg catttgcaga agctgtacga gtaggtgcag
    34681 atggtatcga actagatgtc catctaagca aagatggtta tttgatcgtt atgcacgacg
    34741 aagaagtgga ccggacgact aacggtaaag ggctgattcg agaaaaaaca ttggaagaac
    34801 tcaaaaaatt gaatgcaggg ggcaaattca gcaaagaatt tccagcagct aaagttcctg
    34861 aattgagaga agtgttagga ctgctcctcc aaaaaaatta ccgagggatg ctaatgattg
    34921 aaattaaaac tgatcagtat gaatatcccg gtatcgaaga aaaattagcc gaaacattaa
    34981 aaagtagaga ttggcctttt caacatgcat attgcagctt tcatttggat tctttggaaa
    35041 aaatcgcatt actggaaccg gagatacagc tggacttatt gatgagtact tctgataaaa
    35101 agatccaact agcagcagaa accccttata tagaaggagt acatccaaaa aatacttggt
    35161 tgttcgaaca tccagtccag gcagctgatt ttccaaaagc aattcgtgca tggacgatta
    35221 acgatgaaga agaaatggcc aaatgtcaag aactcaaaat agatggaata ataactgatt
    35281 ttccggagaa agctcttcgg tggaaacaat caacgaaaag taggtaaaat atggaaaaag
    35341 tattagtgat cgtcggtccg acagcagtcg gtaaaacagc gctaagtgtg gaactcgcac
    35401 aactatttgg aggagagatc atcagtggtg attcattgca aatctataaa aatctggata
    35461 tcggtacagc aaaaatcaaa gaagaagaga tgaataatgt tccgcatcat ttgattgatg
    35521 tgatcgaacc tactcaacgt tactcagccg ctgattttca gcattcaggt cgacaattga
    35581 ttaccgagat tgcgaaccga gggcacctac ctatcatagc tggtggtacg ggtctctata
    35641 tccaatccct tctatatgat tatcagttag gagcgacaga agaagcgaat cctgagatta
    35701 gaaaaaagta tgaaacgtta gctgaaacaa ttggttccca agcattatgg aatcagttga
    35761 aagaaaaaga cccggcagct gctgagaaaa tccactggaa caatcaacgc aaagtgataa
    35821 gagctctgga agttttagag acgacaggac atagtatcac ggcacctaaa gaagagccaa
    35881 agcaattgta tgattacctc atgattggat tagatacgaa gcgtagtatt ctttatgaac
    35941 gaatcaatca aagagtcgat ttgatgctag cagaaggttt agtagcagaa gcgcgtaagg
    36001 tgtatgagct tggagaagtc caggccagtc aaggtattgg atataaagaa tttttccctt
    36061 attttgaagg gaaagaaaca ttggaagaag caacagaaca agtcaaacta cattcgagaa
    36121 gatacgcgaa acgtcagttg acgtggtttc gtaaccgttt ggatgcacag tggtttgatc
    36181 ttataagtga gccaaatcaa aaaagtcaga ttgagcaaac aatcaaaaaa tggctggagg
    36241 cggaataaaa tggaagaaaa agtgatcatc gtaggggtag aaaccgaagc aaaccagcgt
    36301 tacttttcag agtcaatgga agagctagtt cagctgacga ataccgcatc aggagaagtc
    36361 gtattcacaa ttacacaaaa gagaccacaa gtggatcggc aaacgattat cggtaaaggg
    36421 aaattacaag aattagttca acaagcggat gcgcatgaag cggatttgat catctttaat
    36481 catgagctaa ctccgagaca gagccaactg attaccgatg ctgttggtct tccggtaatt
    36541 gatcgcgtgc agcttatctt agatattttt gcgatgcgtg ctcgttcgaa agaagggaag
    36601 ctgcaggttg aacttgctca gttagaatac ctgctgcctc gtttagtagg gcaagggaaa
    36661 acattatctc gtcttggcgg tgggatcgga acaagagggc caggtgaaac aaagttagaa
    36721 acagaccgcc gacatatacg gaataaaatt tttgctgtta agaaagaatt gaaagaagta
    36781 gaagctcatc gagagcgcaa tcgccaaaaa agaaaaaata gcgagatttt tcagattgga
    36841 cttattggct acacgaatgc cgggaagtca actatcttga atctattgac acaagcggat
    36901 acttattcga aagatcaatt gtttgcaacg ttagatccat tgactaagag atggcgtttt
    36961 gctgaaggat ttgagattac tgtaacggat accgtcgggt tcatccaaga cctgcctacg
    37021 caactgatcg atgctttcca ttctacatta gaagaaagtc aaaatatgga tcttttgcta
    37081 catgtagtcg atgctagttc tccagatcgt attttacagg agcagactgt cttaaaattg
    37141 atggatgaac tgaatatgaa agaaatgccg atactgactg tttacaataa ggcagaccag
    37201 atcgacccag cgatgttcac gcctacgcta ttcccaaatg tattgatttc tactcagacg
    37261 aaagaaggta agatggcgtt gatcgaagca atcaaacgtc agctgatgga actaatgacc
    37321 ccttatacac tttctgtgcc aagcaacgaa ggacagaaat taagcgagtt gagaagacaa
    37381 acaatggttt tggacgaagc gtacatggaa gaaacaaacg aatatgaagt taaagggttt
    37441 gcaaaaacca attcaaaatg gttaagaaaa aaccaataaa attttatttt tttaa
//
LOCUS       DAYYDS010000018        37245 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000018 DAYYDS010000000
VERSION     DAYYDS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.018"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..839
                     /locus_tag="ACHA02_000917"
     CDS             <1..839
                     /locus_tag="ACHA02_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8669285.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1192..2040)
                     /locus_tag="ACHA02_000918"
                     /pseudo
     CDS             complement(1192..2040)
                     /locus_tag="ACHA02_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2193..3305
                     /locus_tag="ACHA02_000919"
     CDS             2193..3305
                     /locus_tag="ACHA02_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8669286.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="ACHA02_000920"
     CDS             complement(3383..3676)
                     /locus_tag="ACHA02_000920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8669287.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="ACHA02_000921"
     CDS             3844..4158
                     /locus_tag="ACHA02_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8669288.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="ACHA02_000922"
     CDS             4179..6158
                     /locus_tag="ACHA02_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8669289.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="ACHA02_000923"
     CDS             6171..6497
                     /locus_tag="ACHA02_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8669290.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="ACHA02_000924"
     CDS             6513..7031
                     /locus_tag="ACHA02_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669291.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="ACHA02_000925"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="ACHA02_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8669292.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="ACHA02_000926"
     CDS             complement(8467..9972)
                     /locus_tag="ACHA02_000926"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8669293.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="ACHA02_000927"
     CDS             complement(10064..10654)
                     /locus_tag="ACHA02_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8669294.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="ACHA02_000928"
     CDS             10833..12479
                     /locus_tag="ACHA02_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM8669295.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="ACHA02_000929"
     CDS             12528..13247
                     /locus_tag="ACHA02_000929"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8669296.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="ACHA02_000930"
     CDS             13726..14673
                     /locus_tag="ACHA02_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8669297.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="ACHA02_000931"
     CDS             14685..15605
                     /locus_tag="ACHA02_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8669298.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="ACHA02_000932"
     CDS             15646..17103
                     /locus_tag="ACHA02_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8669299.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17448..18062
                     /locus_tag="ACHA02_000933"
     CDS             17448..18062
                     /locus_tag="ACHA02_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8669300.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18055..19779
                     /locus_tag="ACHA02_000934"
     CDS             18055..19779
                     /locus_tag="ACHA02_000934"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8669301.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19776..21227
                     /locus_tag="ACHA02_000935"
     CDS             19776..21227
                     /locus_tag="ACHA02_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8669302.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21700..23853)
                     /locus_tag="ACHA02_000936"
     CDS             complement(21700..23853)
                     /locus_tag="ACHA02_000936"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8669303.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23855..24901)
                     /locus_tag="ACHA02_000937"
     CDS             complement(23855..24901)
                     /locus_tag="ACHA02_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8669304.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25051..26340
                     /locus_tag="ACHA02_000938"
     CDS             25051..26340
                     /locus_tag="ACHA02_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8669305.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26353..29055
                     /locus_tag="ACHA02_000939"
     CDS             26353..29055
                     /locus_tag="ACHA02_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8669306.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29040..29897
                     /locus_tag="ACHA02_000940"
     CDS             29040..29897
                     /locus_tag="ACHA02_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8669307.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29968..30825)
                     /locus_tag="ACHA02_000941"
     CDS             complement(29968..30825)
                     /locus_tag="ACHA02_000941"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8669308.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31036..33207
                     /locus_tag="ACHA02_000942"
     CDS             31036..33207
                     /locus_tag="ACHA02_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8669309.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33275..33616
                     /locus_tag="ACHA02_000943"
     CDS             33275..33616
                     /locus_tag="ACHA02_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8669310.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33892..35646)
                     /locus_tag="ACHA02_000944"
     CDS             complement(33892..35646)
                     /locus_tag="ACHA02_000944"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669311.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35657..36493)
                     /locus_tag="ACHA02_000945"
     CDS             complement(35657..36493)
                     /locus_tag="ACHA02_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8669312.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36495..37178)
                     /locus_tag="ACHA02_000946"
     CDS             complement(36495..37178)
                     /locus_tag="ACHA02_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8669313.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12016 a         6551 c         7639 g        11039 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    17221 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    17281 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
    17341 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    17401 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    17461 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    17521 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    17581 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    17641 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    17701 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
    17761 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    17821 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    17881 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    17941 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    18001 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    18061 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    18121 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    18181 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    18241 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    18301 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    18361 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    18421 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    18481 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    18541 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    18601 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    18661 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    18721 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    18781 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    18841 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    18901 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    18961 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    19021 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgagg
    19081 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    19141 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    19201 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    19261 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    19321 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    19381 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    19441 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    19501 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    19561 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    19621 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    19681 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    19741 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    19801 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    19861 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    19921 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    19981 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    20041 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    20101 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    20161 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    20221 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    20281 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    20341 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    20401 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    20461 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    20521 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    20581 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    20641 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
    20701 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    20761 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    20821 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    20881 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    20941 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    21001 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    21061 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    21121 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    21181 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa
    21241 tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac
    21301 ttgaggcaag gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc
    21361 ggtattaagc caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat
    21421 actcgaaact aaacagcttt ttcacgacct ctccactaaa cggtgttcaa tcagctgacc
    21481 ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta
    21541 atactcaggt cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca
    21601 tctgcggtaa taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc
    21661 ttattacttg atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga
    21721 aaaaggcaaa tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga
    21781 gatcgtgccg cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctctgtc
    21841 catccgtttg attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt
    21901 caattccttt ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac
    21961 atattcaccg gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc
    22021 ggccatactt ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg
    22081 agtcatcaac tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa
    22141 gtaaggatag tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc
    22201 catctcactc atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg
    22261 attgcacagc tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag
    22321 tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc
    22381 ttgtccccaa cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg
    22441 cataaatcct gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt
    22501 cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata
    22561 agcatagtct aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt
    22621 caagtaatct tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc
    22681 tttttccatt gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc
    22741 atctgcaatg accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag
    22801 ccattttggc aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc
    22861 gtatttttct acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa
    22921 cccattattt gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat
    22981 tttttgccca ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa
    23041 attatgataa aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa
    23101 ataggactgc cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc
    23161 tagttccagg ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt
    23221 ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc
    23281 taactcatca attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg
    23341 atcttctgaa ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa
    23401 gagctgatgg cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat
    23461 agataagatt ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg
    23521 acagatcgac agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc
    23581 atggaaaagg gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa
    23641 atcacccatc caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc
    23701 aggatgaaac caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc
    23761 aaaaggaacg ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt
    23821 tgcatgtcta gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt
    23881 tgatgaatga aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga
    23941 ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg
    24001 gcgggtcaac aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag
    24061 ggatatgata accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt
    24121 cacaaacaaa agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg
    24181 cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt
    24241 aaccaaggta acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga
    24301 tttttgaatg tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat
    24361 cggaaagaac ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga
    24421 attctttcaa tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat
    24481 aaatcaatcc gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa
    24541 gcgtcgtcat catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa
    24601 aatcaccaac agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg
    24661 taggagaagc aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa
    24721 cgcgataaat aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc
    24781 ttactttata ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt
    24841 caccagttaa aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgagtca
    24901 tataaacacc tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa
    24961 ataaaaagat attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa
    25021 gatatatcaa atagaaagaa ggttttatta gtggtttata aagatatacc aaaatcggtg
    25081 cagaaattta tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag
    25141 aattttacag cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga
    25201 accacctttt tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa
    25261 gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg
    25321 ttagtcaaaa agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa
    25381 gaagctaacg gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa
    25441 agaaaatatg aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa
    25501 aatacaggaa aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg
    25561 gacgtgttca aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg
    25621 acaagaaaag aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga
    25681 atgacatggt ctggtttccg tcctagcgat gatgcttgcc gttatggata tctggtacca
    25741 tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc
    25801 gatgataaag aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt
    25861 cagaatcacg gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat
    25921 ggaatgggaa acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct
    25981 tacttgggat attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg
    26041 agtaaagaaa atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat
    26101 acgccagaaa aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac
    26161 aaatctgaaa aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt
    26221 atgcacgaag gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg
    26281 gcaaatatga tgttttgcga attagtcatg gattactttg atattagagt agaaaagtag
    26341 gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa
    26401 tggtacatgg cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta
    26461 gagctatttg aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt
    26521 ttagatgact atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat
    26581 gaaggaaaat tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg
    26641 gaatcgaacg tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct
    26701 gtcatgttag gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg
    26761 aaacaagctg gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat
    26821 aatcaagttt tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaaggc
    26881 cctgatcaaa cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag
    26941 attccagtag aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa
    27001 caatatgcct ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa
    27061 aaagatatat caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt
    27121 cattctaatt ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc
    27181 tcagtagaag gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca
    27241 gcatctgctc gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat
    27301 attacagaac cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa
    27361 ttagattatg cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt
    27421 tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga
    27481 aagtatcttg cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc
    27541 ccaaaagaca gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa
    27601 gcagaaataa ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta
    27661 tatcaagagt tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc
    27721 actataccag ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac
    27781 cgtttccgtg tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg
    27841 ccagcatttt cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag
    27901 gaaacaatga ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt
    27961 gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg
    28021 catatttttg aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt
    28081 gatcagccaa tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca
    28141 ccggaaatag caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag
    28201 cgtttagaaa aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc
    28261 aaagaaaaac gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa
    28321 atcgactttg aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt
    28381 cctacaaagt tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc
    28441 ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt
    28501 gtcaatattc atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat
    28561 gaagtaacag aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc
    28621 gattggggat attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac
    28681 agcattgaac tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca
    28741 cagattcctt tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa
    28801 caatatttaa cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt
    28861 agtgataaag tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata
    28921 actaaagaca atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg
    28981 gttgtcccaa taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa
    29041 tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag
    29101 aaattttaga aaaattaagt atccctacca caacaccaga agaaacattg gatcaggtat
    29161 ttgaattttt tgatcgttat gaattgaagg gaatcgggat tggctcattc ggcccgattg
    29221 gtatcgacaa aacaaaagat aattacggat acgttttggc aactcccaaa aaagggtggg
    29281 cacaattcga ttttcttggc acaatcaagg aacgctacaa tattcctgct gcttggacaa
    29341 cggatgtcaa tgctgctgca tatggggaat tgaagcaagg agcagcacaa ggaaaaaata
    29401 gttgtattta ccttaccgtg ggaacaggaa tcggtgcagg tgtcgtgatc aatgaagaaa
    29461 ttttttcagg gatcgcacat cctgaaatgg gacatatttg ggtgaaacgc catccagatg
    29521 accattatga aggtacttgt ccttatcata aagattgttt agaaggatta gcagcaggac
    29581 cgtctattga ggcacgcaca gggatcaaag ggcaagatct gccgcaagat catcctgttt
    29641 gggctattca ggcttattat attgctcaag cactgatcaa ctatacgttg acgttagcac
    29701 ctgaaaagat tattttaggc ggtggggtaa tgaatcagga tcatctcctt caaaaaatcc
    29761 gtcagcaatt tgttgaattg atgggtggtt atatggaaac acctcaagta agtgaatata
    29821 tcgttcgttg gggactgccg aatgaaagtg ggattatcgg tagtttgctg ttagcagaac
    29881 acgaacatca aaagtaagca gacaaattac acaaaaagaa cagagtctta tcattatctg
    29941 ctgataagac tctgttcttt tttatttcta ttcccataat ccaggtaaag ctttaccgac
    30001 tattttttct tgagtaaatg gatggatcat gtgcagttct aatgcatgga gcatcagtcg
    30061 ttcagctgtt ctattttggt agagagggtc tccaacgatc ggatgtccct tatgagctaa
    30121 atgaactctg atctggtgtg tacgtccggt ttccaaaaca caatagactt gtgtttgttc
    30181 agaaagtact ttgttgacag tgacatgtgt caaggcgctc tgaccatttt tatcatctac
    30241 gacccttttt ctccgatcat ggcgatctcg tccaatttta ttggtgatgg tcatatcttt
    30301 ggcaatttct ccccatacga tggcttgata tctccgatag atcatttttt gttccaacat
    30361 tcttccaagt atcggcaaga caaatggatt ttttgcaaat aaaacggcac cgcttgtttc
    30421 tttgtccaag cggtggacaa cgtaaggtcg ttgctgttta gtgtcgagat aagcagctaa
    30481 atgattgagt aaggtactgt tttctgtcgg ttcatttgga tgagtcttca ttcctgcagg
    30541 cttatccact ataatcaaat gctcatcttc atataaaaca cggatgtgct tcgcatctcc
    30601 taattgaact tcttgataat tataatctgt ttcttcgaat aacaatgtga tctggtcatt
    30661 gccctttact tcatgatgga acaaagcagg ttgttgattg atccaaacgt ttttacgtat
    30721 acgcaaaaag tgtcgaacct ttctaggaac taaccattcc ttttcaagca agtcgcgaat
    30781 tgttgtttca ggatggttca taggtaaagt gatcgtgatt ttcacaagtg tactcctttc
    30841 agtgcaattg cagactaatt gtaccatttt taaaacatat ttaagaaaaa acttacattt
    30901 ttctgttcat tcttatgtta ctctacttgc actaacgtaa aaaaacatat tgaactacaa
    30961 cttaagcagg aagaaaacac tctttcacta tggtaaaata agacgattga atcaaataga
    31021 caggagaaga agcaaatgga ttttcaaagc attttagaaa agataaaagc tgctttgatc
    31081 agtttttggc gttggatcag accatatctc ggacaattcc atcgatggcg aaagcgcatc
    31141 tggaaaaaat atcatgtgaa caaattgatt ttgctgattg gtttgatctg tgtcttagtg
    31201 acaagtatct acctctttat tttagcaaaa caagcaaatg tcgaaacgtt aaaatcagga
    31261 ttaagccaat cgacagtcat ttatgataag aaaaacgaag aagctggcac actgtacgcc
    31321 caaaaaggaa catatgtaga attaaacgcg atttctccct atatccaaga tgcagtaatc
    31381 tcgacagaag ataggaattt ttacgagcat cacgggtttg atatcaaagg gattgcccgt
    31441 gcagcagtcc gaatggtctt gtctggcgga acatcagggg gcggaggaag tacgatcact
    31501 cagcagcttg ccaaaaatgc gtatctgaca ttagaccaaa cgtttgatcg aaaagcgaag
    31561 gaattatttt tggcaatcga gatcgaaaag aagtacagca aagaagaaat cttgaccatg
    31621 tatcttaaca atgcctattt tggcaacggg gtatggggtg tccaagacgc ttccagaaag
    31681 tattttggag tagatgcaac gaatgttacg ctgtctgagg ctgctacttt ggcaggaatg
    31741 ctgaaaggtc ctggtattta taatccgatc gatcacccaa aaaatgcgaa tgatcggaaa
    31801 aacacagtac taagtgtaat ggaagaaaac ggaaaaatca caaaagaaca agaacaagca
    31861 gaaagccaga cagatatcag cgcgtatctt aatgacacat acgataattc agattctggt
    31921 tatcgttatc cttattattt tgatgcagtg atcgatgaag cagttaacga gtatggtctt
    31981 gatgaagaag atgtcatgaa taaaggctat aaaatctata cagctttaga tcaggattat
    32041 caaaagcaat tagaagctac ttatcaaaac agtgcattat tcccagcaaa tgcttcagat
    32101 ggtgccatgg tccaatctgc ttcagtagct ttagatccga atacaggtgg tgtacaagca
    32161 ctagtaggac gaagaggaga tcatgtcttt agaggattca gctttgctac acagatgcgt
    32221 cgttcaccag gatcaacgat caaaccatta agcgtatata caccagcact ggaagctgga
    32281 tacaagcctt ccagtatctt gaaagacgaa ccgcaaagtt attacgatgc gcataatttt
    32341 gacgggacat accaaggaga agtaccgatg tatcaagctg ttgcacaaag tttgaatttg
    32401 cctgctgtat ggcttttgca tgagatcggt ttacaaaaag gatatgataa agcagaagaa
    32461 ttcggcctgc ctttagcaaa atctgataag tattatggct tagcccttgg tggactggaa
    32521 aaaggagtat ctccattgat catggcgggt gcttacagtg catttgccaa tgatggtcag
    32581 atgtatacgc cgcatcttat cacgaaaatc gttgattcga ctggagcggt gatcgtcgat
    32641 aacacaaaca aaaaaccaaa acaggtcata tcaaaggaaa cagccgatga aatgaccagc
    32701 atgcttcttg gtacattttc aaatggaaca gcggctgctg cgaatcctgc aggttatacg
    32761 atagcaggga aaacaggaac cacagaaact aatttcgatg ccacaaaagc aaatgaccaa
    32821 tggatgatcg gttacacgcc agacgtagtg atttcgacat ggatgggtta cgaagaatcc
    32881 agcaagctgc actatttgga agggacaagt ggaaatgttg tcgggaaagt cttcaaatca
    32941 gcggcagaag gcatccttcc gtatacttca aaaacaggat ttagtgtagc agatgcctat
    33001 gccacaggag gacaagtggt agctgcagat caagttggaa acacaggaaa ttctaatgga
    33061 gagtctggaa attggcaaga taacttgaat caatatggtg aacaagcaaa agaagggctg
    33121 aaaaacttcg gcgatatggt gaaagaaggc gttcagggaa ttggtgaagc ggctaaagac
    33181 ctctggcgga aatatcaagg agagtagccc ctttgaaaac gaataacccc atgttacaat
    33241 agagagtaac tactaaaaga aaagagggca taacatgtca aacatctacg atagcgcgaa
    33301 tcaaatcgaa cgtgagatcc gtgaattacc agaattcaaa gcgttgaaag aagcgtacga
    33361 gaaagtacaa gcaaacgaag aagcacacaa actgttcaaa gacttccaag cgatgcaaat
    33421 cgaattacaa gaaaaacaaa tgagtggaca agagttcagc gacgaagatg caatgaaagc
    33481 acaagaaatg gctgtgaaaa tccaatcaga agaagtaatc aatgacttga tgcaaaaaga
    33541 gcaaggcttc agtacaatca tcaatgactt gaaccgtatc atcatgacac cagttcgcga
    33601 cctttacagc gaataaaact attttatgaa acaacagagg gtgtgaaaga gacgttcggc
    33661 cttgagcgat gacaaaattg cttcttattg ccgataggct gtctcttgaa caccatttac
    33721 caagaggtcg tgagcctacc gtttaactct gagttacaag gaacaatttc aaaaaacagc
    33781 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    33841 attcagaaat aggagctacg acaaaagtct tgtcatagct cctatttttt gtcattcatt
    33901 tttcaagcgg tagaaatagc ctttttgagc taacagtttt tcgtaagtac ctatttcaac
    33961 gatttttcct tcatcgatca caacgatttt atctgcctct ttgatcgttg aaagccgatg
    34021 agccacaata aaagtagtgg gtgtttctgt caggtaattc agggcttgct ggattttttt
    34081 ctctgactgg ttatctaaag ctgaggttgc ttcatctagc aaaatgattt ttggctgacg
    34141 tatcaacgct cgtgcaatgg agatacattg cttttggcca ccagacagct tgttgccatg
    34201 ttcgcctact aaggtatcca gaccatcagg aagctggtca atcagtgaag aaagttgcgc
    34261 agcttcgatg acttcatcta aacgttcttt tgatacgttt ttcaagccat aagtgatgtt
    34321 ctctttgatc gaagcggaga acaacaaagt ggtttgagga acaacggcta aataatttcg
    34381 cacacttcgc atatccagtt catccaaagg ctgatcatct agaaaaattt ttcctgaagt
    34441 cggctggata aatccaatca ataagttaag gagtgtagat tttccagagc cagatggccc
    34501 aacaaaagca attgtttctc cagctttcac ctctagagaa aaatgatcga taatcggttt
    34561 gagtgagtcc ggataaagaa aagaaacatc ctcaaaacga aaatttccat ccaaatgttg
    34621 gatttttttc ttaccgtgat aagtttctac atcgccagca atcaacagtt cgccaataga
    34681 agtgatagat tccatgcctt ttgaaatcgt aggaatcagc gtgatcattc cagaaacgga
    34741 attgacgatc gtcgtgaaat acgtctgata catcacgaca tcgccgggtt ggatgcgccc
    34801 ttgtaaagct aatatcccag tgaaaatcaa acaaatcagc tgaaatatct gaaaactagc
    34861 ccagctgact gatccgaaaa cagattgcag gatatctaag tggaatcctt tttcatagat
    34921 ttgagtaaaa tgcttattta agcgagttgt ttcgtgtttt tctaacccat gagctcgagc
    34981 aatcggtgct aattcgatca cttcgatcac ttttgcagaa gtctcttcca tttcttgcct
    35041 gaagtctgag ttggactgtc tgatcctttt acggaaaaag agagtaacga atgcggcgag
    35101 aggaacagtc aataagaaaa acatgaaaac aacaggactt ttataaagcg tgatacctaa
    35161 agctacgaat aaattgacag cgatatttaa tccgctgaca aatacttgag aggacaacgt
    35221 ttggatagct tctacatcac ggatgatctt tgactggatt cgtccagatt gcaattcttt
    35281 ttgataagga atagatagtt cttggatttt cttgataagg gtcgctctta gtccagcttc
    35341 tacttgacgg atcgaagtgc tatggtattt tacatgaaaa taattcgtca atatattttg
    35401 accaatcaga caagccatca aaaaagcgtt gaaataaatg gcctgcaact gagaagaatc
    35461 tttcgatgaa gctgcattga ttacattggc aatagcgatc ggcattaacc aagatggaag
    35521 atgtttgata ataaaaaaga gtcctgacat ggcaaaacgc caataatttc cctgataaag
    35581 atgcagcaat gtctgaaaag catttgcatg atatttttga taacttttct gtattcgttc
    35641 ctccataatc ttctccttaa caatcttggt aaataaactg caagtgttct attgcttcag
    35701 gaacataaat ctcaggagta gcttcaagag tggcgtacat tagaggacgc tcatatttga
    35761 tataatgcag taccgggcgg aaatctaaac agcccttgcc aacaggtacc atgcggatct
    35821 ttccattttc catgatatag tctttgagat gaagcgctgt aatataacca tgtaaattgt
    35881 ccaaggcacc tcggatcact ttttcctgat cagcatgatt ttctttatgc atcaaattcg
    35941 cacaatctaa aatgattttt aaatttggtg aagctacttc atcgattaaa cgtttagcta
    36001 actgatagtt atagattgga tgattgatcc ctgcttcaac agcaacagtt acgccaaaat
    36061 tttctgcatg agcgactaat tcaatgatag aatttctggc gatctgataa gcttcttcag
    36121 taaaattttc ttttgtgtaa ccgacagaga cactaccggt ttccgtagca atcatcttgg
    36181 cttgataggc agagcacaga gcaatttgct gtttgaaagt tgccaatgct tgtgcacgaa
    36241 cttcttgatt tttgctggat aggttgatat agcatcctaa aatggaaagt tcgatattgt
    36301 ttttttggaa gtattcacca aaataggcag cactcccaag tgtcatgtca gttaatttaa
    36361 gttgttcagg aaatgctttt tgtggtgcaa attggatatg ggacaagtga agctgtgata
    36421 ctttttgaac gatttcttca cgatttcgaa catctaaatc gtgggcacgg atgcctaaat
    36481 ttaatggaaa catctcaaac tcctttttgt aaataattag caagaggcag acgggattct
    36541 tttatcaatt caacaattat tcgggcaact ttatcagcgc cttctggact aaagtgggta
    36601 ttgtctttta gtccttctgg gtaattttta caagtatcag gcataagatg caggtagaat
    36661 ttttttgtct cttcttttga gaaggtctgg aacaattctt gtgatcttga gaacacatct
    36721 aaaagaggaa tatggtgttt ctcagcaaat gcacgcatcg cttttggata gtctccaagt
    36781 atatctggat tcaatgttcc gttttctaag taatctcgtc gcgtcacaga tgtcagtaag
    36841 accatttgga cgttcgcttt ttttgcgcat tcaaaaaata tctgcagatt ttttaaatag
    36901 ctaccgaagg gaagcgtacc tctttcttct tggattttct ggtcgttatg tccaaattgt
    36961 acaaataaga aatcattcgg ctgaaactgt gaagctagac gggctaacct tccttcctgg
    37021 ataaatgatt tggtgctgcg tccattcttt gcttggttat tgatctggat ctcgggtgaa
    37081 aaataagcag agattttttc tccccaacct gtctcgggac gcttgttttc atctttttct
    37141 gccgcagtag aatcaccggc aatatggata gtaggcatgt tatctcttct ttcataaagg
    37201 tttataggca atgaagcaga acagcaagag aagaagacgg agcaa
//
LOCUS       DAYYDS010000019        35900 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000019 DAYYDS010000000
VERSION     DAYYDS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.019"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(174..2519)
                     /locus_tag="ACHA02_000947"
     CDS             complement(174..2519)
                     /locus_tag="ACHA02_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8669314.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            2798..3700
                     /locus_tag="ACHA02_000948"
     CDS             2798..3700
                     /locus_tag="ACHA02_000948"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8669315.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            3913..5688
                     /locus_tag="ACHA02_000949"
     CDS             3913..5688
                     /locus_tag="ACHA02_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8669316.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5754..7340)
                     /locus_tag="ACHA02_000950"
     CDS             complement(5754..7340)
                     /locus_tag="ACHA02_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8669317.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7591..7815
                     /locus_tag="ACHA02_000951"
     CDS             7591..7815
                     /locus_tag="ACHA02_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8669318.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7957..9708
                     /locus_tag="ACHA02_000952"
     CDS             7957..9708
                     /locus_tag="ACHA02_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8669319.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            9708..11492
                     /locus_tag="ACHA02_000953"
     CDS             9708..11492
                     /locus_tag="ACHA02_000953"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669320.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            11818..12669
                     /gene="licT"
                     /locus_tag="ACHA02_000954"
     CDS             11818..12669
                     /gene="licT"
                     /locus_tag="ACHA02_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8669321.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            12762..14648
                     /locus_tag="ACHA02_000955"
                     /pseudo
     CDS             12762..14648
                     /locus_tag="ACHA02_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(14688..15590)
                     /locus_tag="ACHA02_000956"
     CDS             complement(14688..15590)
                     /locus_tag="ACHA02_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8669322.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            15821..17224
                     /locus_tag="ACHA02_000957"
     CDS             15821..17224
                     /locus_tag="ACHA02_000957"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8669323.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            17224..18657
                     /locus_tag="ACHA02_000958"
     CDS             17224..18657
                     /locus_tag="ACHA02_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8669324.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18802..19728
                     /gene="trxB"
                     /locus_tag="ACHA02_000959"
     CDS             18802..19728
                     /gene="trxB"
                     /locus_tag="ACHA02_000959"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8669325.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19942..21669
                     /locus_tag="ACHA02_000960"
     CDS             19942..21669
                     /locus_tag="ACHA02_000960"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8669326.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            21684..22034
                     /locus_tag="ACHA02_000961"
     CDS             21684..22034
                     /locus_tag="ACHA02_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8669327.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22126..22986)
                     /locus_tag="ACHA02_000962"
     CDS             complement(22126..22986)
                     /locus_tag="ACHA02_000962"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8669328.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            23078..23506
                     /locus_tag="ACHA02_000963"
     CDS             23078..23506
                     /locus_tag="ACHA02_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8669329.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23668..23844
                     /gene="rpsU"
                     /locus_tag="ACHA02_000964"
     CDS             23668..23844
                     /gene="rpsU"
                     /locus_tag="ACHA02_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8669330.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23871..24317
                     /locus_tag="ACHA02_000965"
     CDS             23871..24317
                     /locus_tag="ACHA02_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8669331.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(24471..25850)
                     /gene="brnQ"
                     /locus_tag="ACHA02_000966"
     CDS             complement(24471..25850)
                     /gene="brnQ"
                     /locus_tag="ACHA02_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8669332.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            26556..27527
                     /locus_tag="ACHA02_000967"
     CDS             26556..27527
                     /locus_tag="ACHA02_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8669333.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            27551..29743
                     /locus_tag="ACHA02_000968"
     CDS             27551..29743
                     /locus_tag="ACHA02_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8669334.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            29760..30236
                     /gene="ybeY"
                     /locus_tag="ACHA02_000969"
     CDS             29760..30236
                     /gene="ybeY"
                     /locus_tag="ACHA02_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8669335.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            30220..30615
                     /locus_tag="ACHA02_000970"
     CDS             30220..30615
                     /locus_tag="ACHA02_000970"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8669336.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            30630..31529
                     /gene="era"
                     /locus_tag="ACHA02_000971"
     CDS             30630..31529
                     /gene="era"
                     /locus_tag="ACHA02_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8669337.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            31672..32484
                     /gene="recO"
                     /locus_tag="ACHA02_000972"
     CDS             31672..32484
                     /gene="recO"
                     /locus_tag="ACHA02_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8669338.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            32868..33785
                     /gene="glyQ"
                     /locus_tag="ACHA02_000973"
     CDS             32868..33785
                     /gene="glyQ"
                     /locus_tag="ACHA02_000973"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8669339.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            33787..35862
                     /gene="glyS"
                     /locus_tag="ACHA02_000974"
     CDS             33787..35862
                     /gene="glyS"
                     /locus_tag="ACHA02_000974"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8669340.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        11639 a         6420 c         7602 g        10239 t
ORIGIN      
        1 tgttataatc ctttttttgt gtcttaaaaa agagagaaag tggttgaaat tacatttatc
       61 gtatataaaa caatccgtgt atagatgctg cattttgttc accaccatca aaaaaataag
      121 ggactgtgac aatactattg tcacaatccc ttattactgt gtattctatt aaatcaatca
      181 tctttctcat ctgattcttt agttgcttga ttcgctcttg cataagctga agcggtgttc
      241 cagtaatctg tatagttttc atcggtaccg ccaacgccaa atttaaaggc gcttttcttc
      301 ggtccatttt tcgcccatag cgattcaact gtttttcctg gtgtttggat ggatttttta
      361 ttgacagtaa cttttccagg tgtttgacca gtaaatccag atactttttt cttcatcaca
      421 tcagatgata attcgaattt ctcatcagtg ccaaaaatgt caggttttgc ttggtaagct
      481 tgattgatca gattggcaag ataattagcc gaacgtatcc ttgctttgct gtccattcca
      541 gtattgtcat cgtgccctgc ccagctactg atggtaatag acggagtaga gacaattagc
      601 catgaatctc ggtattcatt ggtagaacca gtttttccga cccaatctgc ttttcctaga
      661 tttccgttta agcttgagat ggcatctttg aatggtgtag tgatttttgc attgatcaca
      721 ctacgcatca tgtcattcat gatcgatgcc gttgcttccg aatagatccg gacagagttg
      781 ctttcgtgtt tataaattac atttcctgcg ttatctgtga tggaatcgat gatatagcct
      841 tcttcataga cgccgccgtt tgctaaagcc tgaaatccgt tggtctgttg aagcgtcgtg
      901 acatctactg tacctaaagg agcagattca acgccccaat tgtcatttgc cgggtagttc
      961 atctttgcca agtagtgctc gtaagcatat tgtttcgatc caccggaatt caacacatct
     1021 tgatataagt gataagccgg gatattgttg gaccattcta gtgactcacg gacggtttgg
     1081 aaggtgttcg atccttcgtt agtagcattg acgattttct cgccagcatt ttctccttct
     1141 tgccaagttg ttgcataatc tgatacacgg gattctgatc cgataagccc catgtcgatt
     1201 gcaggtccat aaacaagaac tggtttgatt gatgagccag cttgacgttc tgtatcgaag
     1261 gcatggttat tttgattctg actgtaattc cgtccgccaa cgaatccgta gatgcggcca
     1321 gttctgttat ccatcaatac atttccggtt tctacttgac tgccagcacc atcatctaac
     1381 atgtatccat aattttcaac gccattttgc aatgcagcat agacatcttt atcgattgtc
     1441 gaatggattg tatatccttt attttggatc gtttgctgtg ccttttgata ataagcgtca
     1501 ctgaccgatg aatcagacat atcagcgttg tctttttcag ccaattgttg tgcaatgatt
     1561 cttgttgctt cattcatcac agtataatac aagaattctc tgtcagtctg ttctgcgatt
     1621 tgttgtggta gaaagtcttt tgtaaggtca taggcttttg cttcttcgta ttgttctttc
     1681 gtaatttggc cttcacgata catactgaat aaaacaaaat cttttctggc aaggccggca
     1741 gaaagatctt cttttagcgc gcctgtgttc gtgtatggac tatacgtgat cgggctttgg
     1801 ggtaatccag cgatataagc agcttgcggc agcgtcacat cttttgcatc tacaccaaag
     1861 attccttttg cagcttcctg gactccagca atgttttgtc ccttattatt acgaccaaat
     1921 ggtgagacat tcaaatacgt agcaatgatc tcatcttttg aaaagtattt ttctacttgt
     1981 gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg tttcatctgt aaggatctgc
     2041 tgtttgacta attgctgtgt caaagttgat ccaccagaag agccaatacc agtagcttca
     2101 gatactaatg ctcgtaaaac agctttagga acgtaacctt ggtgtttatc gaagtattca
     2161 tcctctgtgc tgataattgc agttttcaat aaaggtgaaa tcttatcaga tgaaatagtc
     2221 gtgcgcatca gatctgtctg gatttttgat atctctgtat tatctgcata aacaatcttt
     2281 gacgtttctg taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg caattgtgtg
     2341 tcttcgacga gataggcaaa atagccagcg cctattccaa tacctagagc tgcgaacatc
     2401 aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat aatcttagga
     2461 acaaaccatt tttctttttt tcttctattc tgtttttttc gagtattttt gcgtggcaaa
     2521 agggtcacct caactaatgg ttttatttcg ctaattatag caaataatag aacaaaaaaa
     2581 cagacaaggc tgtttttggc aggaatttct tttttaaaag agttaaaacg tgagaaaatg
     2641 atctttatta taaaagagtt cttaagcttt tctttagaga aaatttcagt tactatggag
     2701 aacagggcaa agaaaaatca atcattttct ttgtatatca tcttaccaat atataggaat
     2761 tgttagtaac aattgccttt caaggaggaa tccatccatg ttattaggct cacacgtcag
     2821 catgagcgga aagaaaatgc tgttaggagc agcagaagaa gcagccagtt atggatcttc
     2881 gacttttatg atctatacag gcgcccctca aaacactaga agaaaagcca tcgaagagat
     2941 gaatattgat gccggaaaag cgtacatggc agaaaaaggt ctaacaaata tcgtcgttca
     3001 tgccccttat atcattaatt tggggaatac gatcaaacca gaaatgtttc cttttgccgt
     3061 ccaatttctg agagaagaga ttgttcgtgc ggaagcttta ggcgctactc aaattacact
     3121 tcacccaggt gctcatgtag gggcaggaac agaagccgga attgctagaa ttattgaagg
     3181 cttgaatgaa gttttgacaa aagaacagac ggcacaaatc gctttggaga caatggcagg
     3241 taaaggtacg gaaatcggtc gaacatttga ggaattagcg caaatcatcg atggagtaaa
     3301 attgaatgaa aaattatccg ttacttttga cacctgtcat acgaatgatg ccggttataa
     3361 tattaaagaa gacttttcag gtgtaatgga agagtttgat aagatcatcg ggttagatcg
     3421 gcttaaagta ctccatatca atgattctaa aaatccgcaa ggttcgcata aagaccggca
     3481 cgccaatatt ggttttggaa caatcggatt cgacacgctg aataagatcg tccatgatcc
     3541 aatgtttgac aatatttcga aaatcttgga aactccctat gtaggcaaag ataaaaagaa
     3601 agcttacgca ccatatggaa aagaaatcgc catgttcaaa gcgcaaacat tcgatccaga
     3661 agtgtttcct gagttattag cagaagaagt gatactctaa gattttctat ctgacagcag
     3721 caaaaaagga ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa ggagtacttt
     3781 ttgcagtctt tttcatcatg catcttctcc cccctctttt taaatttaga caaacaaggt
     3841 atactggtag aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa acgtgattgg
     3901 aaggacgaaa agatgctgaa cactttaaaa cccttgatca accaactaaa aaagaaacat
     3961 acatacttta ttcttggcat agccctatta tttacaatta ttcgtttgta tttatatagt
     4021 aaagcaatct atggaacaga catgtcggca acctatgatg accagctgct gattcgctat
     4081 gctcaaaata ttttggacgg taaatggcta ggggagtata cgactacaac actttccaaa
     4141 ggaatctcct acagcttatt tttagtactt gcacataaac tgcatctttc ttatgcagtt
     4201 ttgttcggca tgttcagtat ttttgccagt atattggttg cactcgcgtt taaaccgatt
     4261 gtgaagaaca agtatcttct tttaagtatc tatttttatt ttttgttttc tccgattagt
     4321 tttacacatg aatattccac tcgaacatat cgtaatgccc tcgtgattcc ggctgttgag
     4381 atcattatcg cttgtttatt agctatttat taccgcaaat acggttctag cagaaaattg
     4441 ctgccatggt ttttagggct ttctgtcgct tttcctttct tttggttcat tcgcgaagat
     4501 tctatctggc ttcttccttt tactgtagta gctttgggta tcagtttcgt ccagatcttg
     4561 ctagggcaag acttttcttt caaagaagta aagaagttaa aaaaacgact ctttcttctc
     4621 tcgaaacggg aatggctgaa actttcattg tttttacttc ctatagttgg cttttccatc
     4681 gtctatcaat ggatcgaata taaaaatgag acaacatacg gtattgcaac catcaatgac
     4741 cgttccagtg gagaatttgg acgtttgatg aaacaactga tccgaataga tgatgaaacg
     4801 aacttgaata aagaaaacag tgagatatgg gtctcacatg ctgcactaaa taaagcatta
     4861 gccgcatctc ctacattcca tactgtttct acccgtattg acgaattata tacgactcac
     4921 gcgtggactc aaggtggaaa agtaaaagaa ttacccggag atatcatctt ttgggcattg
     4981 cgagatgtgg taagcgaatc tggttattac caaaacaatg caaaagaaac caatgatttt
     5041 tggaaaaaag tcaaccaaga attagaagaa gcctatcaca agggaacaat caaaaagaaa
     5101 aaagagttct atctgatggg atcaggagat ggaaagacga tgagtgatct gccaatcgta
     5161 ttcgatttcc ttcaatcagg atacgcttat gcatggcgat ataaagattt ccatcaagga
     5221 ggaaattata gtttcggaac gcaagaacaa gtcaaacagg ctcaagaatt attaaacctt
     5281 ccgctaatgg agaattggat cgatagtaat gaaccgagaa cacctaattt ccaattaaca
     5341 aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc aagttctggc tattcccgtg
     5401 ctgattgctt caatagcagg gatattactt atgatactag gaaccgtatt tgcaaaagct
     5461 gatcgtgcct attatagttc ctctttgatt gtggtcaccg gattaatact gacacaattg
     5521 gtttttcttt tcggtgtctc atggttttgt tcttatgcgc cagaaaaaaa ggactatttt
     5581 ttaagtgtct acactggtgc aggcgtgcca ttaacgcaat tcatcgtagt aacaagcatg
     5641 ctagttttca gtaaattaaa tctgaaaaaa gctttatctt ctaaataaaa agacccttgg
     5701 tagctagatt tttaacctaa ctatcaaggg tctttgtgtg gaaaaatcag tttttattcg
     5761 atttcttcta ccgtttcctg caaaagctgt tcatattttt tgtttccttt atataaatca
     5821 ccaatcgtcc atgccaacag gagaaggaca agaacaatga ttacataagc gatgtgatca
     5881 gccaatgcta gttgagcgga agtggcttgc tcgccgaaga aactttcgat ctgtcctggt
     5941 aatcctcgta agttcaagta agttagacta atcacagaga accagccaag aagttttacg
     6001 atgaatgaat ttttaaatcg gctacccatc tccgcttggc tgttggtcat catcagtaaa
     6061 ggaatcatcg aaaacggcaa cgcaaaagcc aagaacacct gggattcatt cattaagtta
     6121 ttcaaagcgg catgttcttc gatcgttcct tttgtacttg taaacaaaac acaaatcaat
     6181 acaggaatca cagaaagcaa tcgtgtgacc agtcttctta gccaaatcgg gatacgcata
     6241 tggatgaatc cttccatgat gacttgaccg gtcaacgtac cagtaatagt agagttctgg
     6301 ccagaagcaa gaagtgctac agcaaataaa gtggacaaga taccagattt tgctacagca
     6361 atcaaaatac cattgctcat agctgatgga tcagataaag cttcatacag accaaaaaag
     6421 gaaggatctt ggacagaacc ggttttgaag acagcgacac ccatgatcaa tagcagtgca
     6481 ttgactagaa aagccattga caactggata ttagaatccc aagtagaaaa acgtacagct
     6541 tgtgctacat cttcttcgtt ttggtgatcg atcttccgag tttgtgaaac tgctgaatga
     6601 agatataagt tatgcggcat gactgttgca ccaataatcc ctaaagctcc agtcagcgga
     6661 gtttcgccgc cgatgaccgg tgagtctgag aatgtatgac tgttagggat aaagccagca
     6721 aataaactgt gccaatctgg gttagacaag ccaacttgat agacaaaaac gaataaaatg
     6781 acgaaaatta agcagacaac cagtgcttcg atcttacgaa acccgacttt tgtcagtagt
     6841 aagagaacga aaacatctaa tacagtaata aagacggcaa tcaccaatgg aatatcaaaa
     6901 agtaagtaga gggcgattgc tgcaccaatg acttctgcaa tatctgttgc cataatggct
     6961 aattcagtca agatccataa aatgacacct aatgtcttac ttgtgcgtgc tcggattgcc
     7021 tgtgccaaat ccatttggct cacgattcct aattttgctg ccatatactg taaaagcata
     7081 gcaatcagac tagacatcaa aataactgac atcaataaat attggaaatt ttgtcctccg
     7141 gtgatcgaag tagaccagtt acccgggtcc atatacccaa ctgctactaa cgcaccaggt
     7201 ccagagtaag caagaagtgt tttaaaaaaa cttttcccct ttggtacatc aactgttcca
     7261 tttacttcag ctaaagataa accatttgta tgttcaataa gtcgatgctt atgaaattgt
     7321 ttattggatt gattattcaa gaaaaaacca cctttcctat ttatctacga ttacattcta
     7381 cacctcaaaa agaaaaaatt aaagaaaaag attcggtacg cctaattttt tttcatggcg
     7441 cgtcttgcta gatgacgaaa gaaattttgt tctcctctaa aaaaaccact attttttgca
     7501 tgaaactaaa gaatacgcct ttcttttttc aataaacttt agtaaaataa gtcattgaga
     7561 gcaattcgga aatagaaagg agagatttat atgttatcaa caggtattgt cgtacttatt
     7621 gctattattg ctttgctaat cggcgcagta ggtggctttt tcctagcgcg taaatatatg
     7681 aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca tgttgcgatc aatgatgatg
     7741 tcaatggggc aaaaaccatc tgagaagaaa attcgccaga tgatgcagca aatgaagaac
     7801 caagggaaaa aataggttct gacccaacga ctttttacgg gctgattttt tcagtccgtt
     7861 ttttatttcg taatttgtta gactattcag attattcagc aaaaaagaca agcacagaca
     7921 atcctaggct cttgataaag acaaaggagg agtaaaatgt cgatttttag aaaattaggc
     7981 tggttttttc gtcaggaaaa gaaacattat ttaattgggg ttttctcgct attcgcagtg
     8041 gccttggtac agttagttcc tccaaaagtg attgggatca tcattgatga gatcgccgat
     8101 aaaaatttat caataaagat cattttcggg tggatcttcc tattgattat cgctgctttt
     8161 ttacaatatc tttttcgtta tatctggcgg acgaatattt ggggaagtgc agctcgattg
     8221 gagaaagaac ttcggcagca gttattcaaa cattttacag aaatggacaa tctcttttac
     8281 caaaaatatc ggactgggga tttgatggct cacgcaacga atgatttgaa tgcgatccag
     8341 aatgtagcag gagctggtat cttgaccttt gcagattcag tgattacggg cggaacaacg
     8401 atcattgcga tgattttgtt tattgattgg cgcttaacac tgattgcgct gattcctctg
     8461 cctttgctgg ccgtcacttc acgagtgctg gggtcaaaac tccatgatgc ttttcgtgat
     8521 tcgcaagaag ctttttctag tatcaatgat aagacacaag aaagtatcac tgggatcaaa
     8581 gtcattaaaa catttggaca agaaaaagaa gatctagcgg attttgaaga aaagattgaa
     8641 gatgcaatta ttaaaaacaa acgagtcaat tttttagact ccttatttga tccattcatt
     8701 accttgatta tcggtctgtc ttatgttgca acaattattt taggtggaaa gtatgtcttg
     8761 gaaggcacga tctcgatcgg gcagatggtc tcatttatta gttatatcgg tatgcttgtt
     8821 tggccgatgt ttgcgattgg tcgattgttc aacgtattgg aaagaggaaa tgcttcttat
     8881 gatcgcgtca atgaactttt gcatgaaaag acgcatatcg tggaacaact gggtgcagtg
     8941 cagaccccgg caaaaggcga gctgaatgta gctatttcct cttttaatta tccgaaagaa
     9001 gaatcaccag cattagaaca aatcaagttt gcgatccaag aaggagaaac attaggtatt
     9061 gttgggaaaa ctggtgcggg aaaaacgacg atcatcaaat tactgatgcg tgaatacgat
     9121 gattatcaag ggaaaatcca atttggcgga cgttcgatca aagaatattc attgaatgct
     9181 ttattaggat caatcggcta tgtgcctcag gatcacttct tgttttcgat gaccattcga
     9241 gataatatcc gtttttctaa tccaaaactt tcagaagaag aagtagagca tgcggcttct
     9301 ttagcattta tcaacaatga aatcaaggaa ttaccaaaag gttatgatac cttagttgga
     9361 gaacgtggag tctctttatc tggcggacag aaacaaagga tttcgattgc tcgagcattg
     9421 atcaaaaatc ctgaattatt aatattagat gatgcactct ctgcagtaga tgccaaaaca
     9481 gaagaagcta tcttgcataa tttaaaagaa ataagaaaag agaaaacaac tatcattgca
     9541 gctcaccgtt taagtagcgt gatgcatgca aaagaaatca ttgttgtcga tcaagggaaa
     9601 atcattgaac gaggaaccca tcatgagctg cttgcactag gcggctggta tgaacaaatg
     9661 tgggagaaac agcaattaga agcgaagata gaaggaggcg aggcctgatg gatgaaaatt
     9721 atcaatcgga atggacaaaa tcgattccac ttaaagaaca gaaaaagatc atcaaacgac
     9781 tgatgaagtt cgccaaacct tttagaagaa cgttcatcgt tgctatctta tttgcgtttg
     9841 ctttgtctgt gatcaatgtc ctattgccaa ggatcatcca aacttttatg gatgatcatc
     9901 tggctaaaca gtcggctacg acacaagtta ttttgttttt tgctggtatg tacttatttg
     9961 gagtcatagt aaaaagtatc atttggtttt tccaatggta tttgtattcc atggcctcat
    10021 tgaagaccta tcagtatatt agggtaaaat tatttgaaaa gctgcataca ctaggtatgc
    10081 gttattttga tcagacaccg gcaggatcaa ccgtttctcg agtgacgaat gacacagaaa
    10141 cgctatttga attttggtac gtctttttga tggtattgac tgggatcttt gcagtcgttt
    10201 cttcattcct tgccatgttt caaatcaatg gagaaattgc cttgtggact ttggtgtttt
    10261 tgccgatttt aggaatcgtc atctggtatt accagaaatt cagttctcgt atttaccgaa
    10321 atatgcggga aaaattaagt cagctgaaca cgaaattgaa tgaatcgatt tccggcatgc
    10381 gtattatcca gcagtttcgc caagaagctc gtttagctaa agaatttgaa caagtgaaca
    10441 atgagtattt agacacgcgt tatgcgatga tcaaaacgaa ttcgattctg ttaagtccga
    10501 tcatcaattt tctatatgca ttggcgattg cgttggtgtt gactttgttt gggatcgatg
    10561 cgttgcattc tcccgtagaa gttggactga tctatgcatt tacgacctat gtccagggat
    10621 tttttaatcc aatgtctcaa atgatggact ttttgagcat cttcacagat gggatggttg
    10681 ctggtagtcg tatcttgaag attatggata cggaagaata tgcaccacaa caaaaccctg
    10741 gagcagatca aatgattact tcaggtaaga ttgagtttcg caatgtaagc ttttcttatg
    10801 atggagagca caatgtatta aacaatatct cttttgttgc taatccggga gagaccgttg
    10861 cattagtggg acacaccgga agcggaaaaa gctcgatcat caacgtattg atgcggtttt
    10921 acgaatttta tgaaggtcag atattgattg atgatcatga cattcgagaa tatccaatgg
    10981 aagagttacg taagaaaatg gggctggtct tgcaagatgc tttcttgttt tatggggata
    11041 tcgcaggaaa tattcggatg atgaatccag ctatcacaga cgaacagata cgccatgcgg
    11101 ctgagtttgt tcaagcaaac aaattcatcg atgaattacc aaaaggatat cactcaaaag
    11161 tgatcgaaca tggtgccagc tattctagtg gacaacgaca gctgatcact tttgcgcgaa
    11221 caatcgtgac tgatcctaaa attctagtat tggatgaagc aacagcaaat atcgatactg
    11281 aaacagaagg attgatccaa gaaggattgg caaaaatgcg tcaaggacgg acaacgattg
    11341 cgattgccca tcggctgtca acgattcgag atgcagaatt gattttagtt ttagacaaag
    11401 gtcatatagt agaacgcggg aatcatgaaa cacttctagc gcaaaatggt ttgtatgcag
    11461 atatgtacaa actgcaaagc agtggtatat aaaagatgtt gtgacagtag aattttgacc
    11521 taagcactag agtgaaaata aaacatagct tctcatattt ttcatctgtg agagttagtg
    11581 tcgtagtcac cagatttaaa aaaagattcg acagctagtc agaagtgcct aatagccact
    11641 aagaaagcgt tgacaaaagc atttgctttc gataatataa agaaaacgat acaacttgga
    11701 tagtgattgc ctgatgcaag ctaaacctga tgagtctccc atggtttcat ggacggcttt
    11761 ttgggttttt ctttttgttt tggatacagc cctatacatc atggaaagga gcaaaacatg
    11821 ctgattgaga aagtactgaa taacaacgtc gtaataacca gggatgaaag aaatcaggaa
    11881 atgatcgtga tgggaaaagg tttggcattc aaaaagaaag tgggtgaccc gatttttgac
    11941 tcatgcatcg acaaaaaatt tcagttgaca aatgacgggc tttcacaaca gttccaagaa
    12001 ttggcacaag cgattcctct tgagtactta gaaatttctt gtgaaatcat tgaatataca
    12061 aaagaggctc tacaagtcca gttgaatgat tctgtgtata ttgcattgac ggatcatttg
    12121 tatacggcta tcgaacgtca caaaatggga atatttgtac caaatgtatt gctgtatgat
    12181 atcaaacaac tatttcctaa agagtatgcc atcgggaaaa aaacagtgaa gaaaatctcg
    12241 gataaatacc acatcaagtt atcagaagat gaagccggat ttatcgcatt gcatttagtc
    12301 aatgcacaag gcattcaaag ccagcaagac aatacctacc tgtttactat aacaatacag
    12361 gatattctaa atatcgtccg ctattactat aagattacat ttgatgaaga ttcagtccat
    12421 ttttttcgat tcgtcacaca cttacgtttt tttctgacac gcgtcctaga tggagaaaaa
    12481 tcagaagtgg gagaagtttc tgaggaattg tttcaggtta ttcaaaaaaa atataaggat
    12541 acgtttggtt gcgttgagaa aatagcagac tttttagccg agacgttcaa ctaccagatg
    12601 tccacggacg aaaaaatgta tttaacgatc catactgcgc gtttagtgca tgagaatcgt
    12661 tgtaaataaa aaaaggagga taaattaaca agaacgtatg aaataagggt agtgacattc
    12721 agttggctaa accttccact aataaattgg agggaataga aatgggaaaa taccaagaat
    12781 tagcgagaaa aatcgtagaa aatgtcggcg ggaaggaaaa tatcaatggt ttgacacatt
    12841 gtatcacaag gcttcgtttc aaattaaaaa acgaagaaaa agcaaatgat gagatcttga
    12901 aaaatatgga cggtattgta acggtaatgc gagcgggagg gcagtatcaa gttgtcattg
    12961 ggaatcatgt gccagtcgtg tttgaagagg ttataaaagc aggaaacctt acttttgatg
    13021 aggctgtttc aacagaaaaa atgagaccgt ttgatatgct tattgatgga atatcgggct
    13081 gtttccagcc attccttgct atcctagctg ctggcgggat gattcgtgga ctgacagcat
    13141 tccttgtctt cttaggcgcg tttgaccgcg gttctggcac atttgttatg tttgataata
    13201 tcggtgattc tgtattccaa tttatgcctg ttattatcgg tttaacagct gcccgtaaat
    13261 ttaaagtaaa tgaattcgta gggatgttgc ttggcgcagc attaatgaat cctagcttgt
    13321 cgctggaagc tttgagtggt gcagcagaag caccactaac gaccatattt tctggcacta
    13381 tttttgaagc acctatttat caaacagtat tcggcattcc ctggattgcg cgtaactacg
    13441 cttcttctgt cattcccatt atctttattg tgttgttagc tagccaaatt cagaaaccaa
    13501 ttaaaaagtt agtacctgag atgatcgcga atttcttcgt tccattcttc accgttctta
    13561 ttacaatgcc tttaggtttt ttactcgttg gaccagtatt cacattcgca actgatattt
    13621 taatggctgg atttgaaaca cttttagcac tttcgccagt tatttatgga gcaatcgtag
    13681 gcttcttctg gcaaattctg gtgatgtttg gtctccattg ggcaatcgta ccaatgggat
    13741 tgatgcaatt ttctgtgaat ggttggcaaa atattatgac acctgttgca gtagtgtctt
    13801 ttggacaaac tgcagcactt accgcactct actttaagct tagaaatcca aaagacaaag
    13861 caattgcaat accagccatt gtttctggta ttgtggggat tacagagcca gctatttatg
    13921 ggtttacatt accacgtaaa aaaatattta tttttacctg tgtcggtggt gcgttagggg
    13981 gcgcgtatag tgggttaatg aatttaactt cttggaatca aggtggacta ggcatcttta
    14041 caattcccaa ctatattaga ccagatggtg atttaacaga tgtgattaat gtattgattg
    14101 ggatcgccat tgcgatggtg gtatccttta cgctgacttt tttcttttgg aaggatgaag
    14161 caggtgaaac agatgatata cagaaaaaat caggtaaaga aattgtaaaa acaccgatac
    14221 aaggacaaat cgctccacta aatgcagcaa aggatgcagc ttttgcacaa ggaacactag
    14281 gtcgaggaat cttgatctac cctgaaaaag gagaagtacg tgcccctttt gacggaacag
    14341 tcatgaccct gttcccgacg aaacaggcaa ttggaatggt ttctgaaaca gggctggaat
    14401 tgctgattca tgtcggattg gatactgtgc agttggaagg gaaatatttt gaaagcttag
    14461 tgcagcaagg ataaagagtg aaacaaggcg atctgttgct acgcttcgac aaaaagaaaa
    14521 tcgaaaagga aggatacagt ttagaaacac ctgtgatcgt caccaattac acggattatt
    14581 tggatatttt ggaaaaccaa ggggagacgg tcgggccaga cgatgcactt atcaccgcac
    14641 tgacctgaac ggttaggtga agaatggact aggcttaact attcttttta ttccacttct
    14701 ttattttcaa aattgaaagg ctctttcatg atggctgagc tgatagggcg tttatcgaat
    14761 ttttgaccga cagaaattag atggactaaa ttctgttgat aattttctgc aaaaacacaa
    14821 ctatataaca cgtctagaag ataacaaatt gatgtattga tcgtaaaatt accaattttt
    14881 gaatacagtc gctcgcgtgt cgtaagtcgc aaagcggctg tactcattgc agatagcgtt
    14941 gaatcaccga tactggtcaa agctaaaatt ggaacatgat tttgtttcaa tatttctgcg
    15001 gtacgaagga tcacattgtt ttcacccgta taagaaatca gaatcccgca catatcactc
    15061 gtgcaattgt acgcttcata gaatgattca ccaaaagtgg tgacaatcgt gacatccatt
    15121 ttgatgcgct tcattttcag catgaaatct tgagaaatca aagtattcga gttactggcg
    15181 aaaatcacga ttttttttgc ttgaagcatt agttgtttgg ctttttgcag ttggtcatga
    15241 tgaatgagag aaagtgtatc atgaatcgtc atctgttcta aagaagccat tttatttgcg
    15301 atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg catctacgtc ttgaaaattc
    15361 ccttcaagat aggctgtttc agctaaaaaa gcattacgaa aactgaccca gccatcaaaa
    15421 cccatttttt ttgctacacg gatgagtgta gaggggtgga cataacatcg ctcagcaatc
    15481 gcttggatgg tcatttcgcc aagtgctgga gaatcgctta agagaaactg gatcacttca
    15541 ttttctgaag gagaaaagga gatttctttg attttttcac gtaataacat gtagatacct
    15601 tcttcttatt tttttacgta aattataact tttttgaaaa tgatttgtaa acggatttat
    15661 actatataac ttaaaataga cattttttga caagatattt acaaaaatcc actagaaaac
    15721 agtaaatttt gtaaatgtta ctggaaagaa acagatttct attgtttgtt ttgtttacaa
    15781 aaaagataat gaaagtgtaa aaaagtaaag gagtgagaac atggcaaaag gaatcaaaat
    15841 cgctacgatt ggtggaggca gcagttacac ccccgagttg atggaaggat ttatcaaaag
    15901 atatgaggaa ttaccgatac gagaaatctg gctcgtagat gtagaagaag gaaaagaaaa
    15961 gcttgagatc gttagtcaga tgtctcagcg tatgtgggat gcttctccgt atgaagtcaa
    16021 gatctatgca actctggatc gggaagaagc attgaaagat gcggattttg ttacgactca
    16081 gtttcgtgtc ggtcaattag atgcacgaat caaagacgag cggatcccta gttattatgg
    16141 gatgattggg caagaaacaa atggtgctgg cgggatattc aaagcttttc gtaccgttcc
    16201 agtcgttttg gcaatcgttg aggacatgaa gcgtttatgt ccaaatgctt ggctgatcaa
    16261 tttcgcgaac ccaagtggga tggtgacaga agcagttatc agatatggca aatgggataa
    16321 agtgattgga ttatgcaatg tgccagtgat ggcgatgatg acagaaccag caatgcttgg
    16381 caagaaaaaa gaagagctga tctacaagtt tgccggtcta aatcatttcc attggcacaa
    16441 agtagcagat aataaaggaa atgaccgcac ggcagaattg attgatcgtt tatacgatga
    16501 agagaatgga ttaccgaaaa atatctttga tatcccgttt ttcaaagaac aattaaaaca
    16561 aatgaacatg atcccttgtg gctaccaccg ctattactat cgtgaagaag aaatgctgga
    16621 acatgcttta gaagaatatc aaacgattgg tacaagagcg cagcaagtga aagaaacaga
    16681 agcagagcta tttgaattat acaaagatcc agaattagac cataaacctg aacaattgca
    16741 aaaacgaggt ggcgcttatt attcagacgc tgcctgtgaa acgattgctt caatctatgg
    16801 aaataaagaa acacagatcg tggtatctac aaagaatgat ggagctgttc ctgacctgcc
    16861 ggcagattgt gtagtagaag tcactgccta tgttggcgca caaggagcga gaaatgtagc
    16921 gtttggttct cttcctgctg ctgaaagagg ctggttgcaa gtaatgaaga atatggaatt
    16981 gctgacgatc gaagcagctg tgactggtga ttatgggaca gctttacagg cgtttacgat
    17041 caatccactt atacgatcag gaaagacagc aaaacggatc atggatgagt tgtttattgc
    17101 acaccagcag tatttaccgc aattcaagga gacaatcagc cgattaaaag aagaaggaat
    17161 cgaagttcaa gatgaactgg caagagaatt atcaggtaat acacatataa tggaggaagt
    17221 ataatgggat tcagaaaaga ttttttatgg ggcggtgcta cagcagccaa tcaatgtgag
    17281 ggcggttatg atgaaggtgg acgcggacta gcaaacgtag atgttgtgcc aatcggaaaa
    17341 gaccgttttc ctgttatcac cggaaagaaa aaaatgtttg aatttgatga ggatcatttt
    17401 tatccagcaa aaattgcaat cgatatgtat catcgttata aagaagatat tgctttgttt
    17461 gcagaaatgg ggttcaagac ttatcgaatg tccatcgcct ggagtcgtat ttttccaatg
    17521 ggggatgaga aagagccgaa tgaagctggc ttgaaatttt atgaggatgc ctttaaagaa
    17581 tgccaaaagc acggaattga gccacttgtg acgattacgc attttgattg tccaatgcat
    17641 ttgatcaaag aatatggtgc gtggcgttcc agagaactcg ttggttttta tgaaaatctg
    17701 tgtcgagtca tcttcaatcg ctataaagga cttgtcaaat attggctgac attcaatgag
    17761 atcaatatga tccttcatgc accattcatg ggcgcagggc tgtattttga aaaaggagaa
    17821 aatgaagaac aaatcaaata tcaagcggca catcatgagt tggttgcaag tgccatcgca
    17881 acgaagattg ctcatgaagt agatccggag aacaaagtag gctgtatgct ggcagcagga
    17941 gcatattatc catacacttg caatcctgaa gatgtttggg cttctagaaa atcagatcga
    18001 gaaaattatt tcttcattga tgtccagtca cgaggagaat atcccgctta tgcattgaaa
    18061 gaaatggagc gaaatggcat tcagatcgaa atggaaccag gggatgccgc attgctgaaa
    18121 gaacatactg ttgattttat ctctttttct tattatgctt cccgagtttc gactaccgac
    18181 aacgaactct tggaacagac tgcaggaaat atctttgctt ctgtcaaaaa tccatatctg
    18241 caagcaagtg aatgggggtg gcaaattgat ccattaggct tgagaataac gatgaatgat
    18301 atttatgatc gttatcaaaa accattgttt attgtagaaa acggcttagg tgcagtcgat
    18361 acgccagatg aaaatggtta tgtggaagat gattaccgaa tcgattacct ggcagcgcat
    18421 atccaggcga tgaaggatgc agtagaacta gacggagtcg atctattagg ctatacgact
    18481 tggggatgta ttgatttagt atcagctggt accggtgaga tgaagaaaag gtacggattc
    18541 atttatgtcg atcgtgataa ccaaggaaat ggcacattag cccgttcaaa gaaaaaatca
    18601 ttcaactggt ataaaaaagt aattgattca aatggagaaa atctttcgaa cgattgatca
    18661 atgatttata aaagtctatt gtttttcagc ggatttttcg agtataataa agctattcga
    18721 gaaacgcaag cagaacaata gacttttttt cgcattggct gtaaagtatg cttacttgat
    18781 taagtaaagg agaatctaca tatgtatgat gtaatcgtta ttggcgcagg tcctgctggg
    18841 atgactgctg ctttatatgc ttcacgttca aacttatccg tattgatgat cgaacaaggt
    18901 gcccctggcg gccaaatgaa caatacggca gaaatcgaaa attatcctgg attcgattcg
    18961 atcatgggcc ccgatctagc ttataagatg tatgaaagtg tctcaagatt tgggacagaa
    19021 aatgcttatg gaatcgttca agatatcaag gatcacggca gccataaaga agtcatctgt
    19081 gaagataaaa gttacgaagg aaaaacagtc attatcgcaa caggatgtat ccaccgtaaa
    19141 ttaggcgtac ctggcgagga agaatttgct ggacgaggcg tttcttactg tgcagtctgt
    19201 gatggtgcgt ttttccgtaa caaacgtcta gtagtcatcg ggggtgggga ctcagcagta
    19261 gaagaagcca tctatttgac acaatttgct tctgaagtag tgatcgtcca ccgtagagat
    19321 gaattacgtg cacaaaagat catccaagac cgtgcatttg caaatgaaaa aatttccttt
    19381 atgtgggata cagtcgttga cgaaatcgtt ggaaacgaaa tggtagtgac aggtgtgaaa
    19441 gcacgtaatg tcaaaaccgg caatgaattt gatttagcag cagacggagt attcatctat
    19501 gttggactgg atccattaac tgcacctttt agagaagcag gtttgacgaa cgaagctggc
    19561 tggattccaa cagacaacga aatgaaaacg aatatgccag gcgtttttgc catcggagat
    19621 gttcgtgaaa aagatttgcg acaaatcact acagctgttg gtgacggcgg tattgctgga
    19681 caacaagtgt ataaatactt ggagtcttta gaagaaacaa cagcataagt ttaaaaagag
    19741 tagaagaacg ggttcaattg ttcttttact cttttttttc gcgtgaaatt tactgaatct
    19801 gatttatgcg aacacgtcca acaccttttg ataaaagaat cctttcttga tttaagcgtt
    19861 ttttggtcct catactttca tatctaataa gtgaatgcta tacttattaa gaaccatatt
    19921 tcagaaagag gtgttatttt catgtcttgg gaacaagtgt atgaacaatg ggtacatgaa
    19981 gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc tgggtgaaga tcctgaaaaa
    20041 cttgaagatg ctttttatgc accgttggaa ttcggtactg caggtatgcg gggaatctta
    20101 ggacctggga tcaatcgaat gaacatctat acggtaagac aagcaacaga aggactagca
    20161 cgtttcatgg atacacaagg tgaagaaaca aaacgccgag gtgtcgctat cgcttatgac
    20221 tcacgccatc agtcaccaga atttgcaatg gaagcagcca aaacactagc acatcacaat
    20281 atcccgtctt atgtattcga aagtctgcgt ccaacaccag agctttcttt tgcagttcgt
    20341 catttgaaga cgtttgcggg aatcatggta accgcatctc acaacccagc ggcttacaat
    20401 ggatataaag tctacggaga agatggcgga caaatgcctc ctgcagacgc agatgcattg
    20461 acgaaatatg tccgtgaagt cagcaatccg ctaaaagtcg aagtattatc tgacgaagaa
    20521 gctaaacata gcgggttgat cacgatcatc ggagaagaag tggacgctgc ttatctagaa
    20581 gaaatcaaag cagtgacgat tgatcgtgaa ttggttgaaa caatggggaa agatttgaaa
    20641 cttgtgtaca cgccacttca tggaacaggg aaaatgttgg gcgaacgtgc gttgaaacaa
    20701 gctggattcg aacaattcgt actagttccc gaacaagctg tggcagatcc agatttcact
    20761 acggtcaaat cgccaaatcc agaagaacat tcagcttttg aatatgctat acgacttgga
    20821 gaaaaagaag gagctgactt gctgattgca acagatcctg atgcagaccg tttaggcgca
    20881 gcggttcgtt tgccagatgg ttcttatgaa gtattgactg ggaaccaaat cggagcattg
    20941 tttgctcaat atattttgga agcacatcaa tcaagaggca ctttaccaga aaacgcagcg
    21001 gtattgaaat caatcgtttc cagcgaattg cctacagcaa ttgcggaaca ctacggtgct
    21061 accatgttca atgtgttgac agggtttaaa ttcattgctg aaaaaatcca acaatttgaa
    21121 gaagaccatt cacatacttt tatgtttgga ttcgaagaaa gttatggtta tttagttaaa
    21181 ccttttgttc gcgataaaga cgcgatccaa gcattgcttt tattagcaga agtagcagct
    21241 tactataaaa aacaaggcaa aacgttgtat gacggcttgc aaacgatttt tgaaacctat
    21301 ggctatttcg cagaaaaaac gatttctgtg acaatgagcg gacaagaagg ttctgcgaaa
    21361 atctctgctt tgatggaaaa attccgtaaa gaagcaccga cagaatttgc cggagcaaaa
    21421 gttattcaaa cagaagactt taaagaattg acgagaacag accgtgaagg aaacgtagaa
    21481 aaactaacaa caccgccatc cgacgtattg aagtatacat tggaagaaaa tggctggatc
    21541 gctatccgcc cttcaggaac tgagccgaaa atcaagtttt atattggtgt aaaaggaagc
    21601 tctacagaag acgcagccga aaaagtagcc tcttatgaag cgattatcaa aaagatgaca
    21661 gaagaataaa aaacttgaga ctgatggaaa aagtggtaaa ctttagaagt cagtcttttt
    21721 tcaagttgga ggtgaatgga ttgccagaca cgcaaaaatc aaaagtagag aaagtcagtc
    21781 aattattcaa aatgttgagt gatcccactc gtcttaaaat tctgctatat ctgaaagacg
    21841 gggaacaaaa tgtgacagca atcacccaag cagtggaaat ggagcagtct gctgtatcgc
    21901 atcaactacg tttattaaga gaaaatcatg tcgtcaagtc tcatcgcgag ggaaaagcga
    21961 ttttgtatag tcttgatgat tctcatgtac tggatatttt gaaccagaca ctcaaacatg
    22021 tggaacatca ataacgatag gtgaaaatag ataaacactg gagtgtgaca acgaagaaat
    22081 ttcgttgtca cactccagtg ttttttaaga aaaagacaaa aatgattaat cgcttttttc
    22141 tgttgtttca gttccataat aactatgatc ttctaggtct aaagcattca aaataagcga
    22201 agccgtttct tcaatagata aagatgccac attgatcact acacatccta gtttttcata
    22261 aagatcattc gcaaaatcga gttcagctct aattttttcg atatcggaat aagcagtatc
    22321 agggtttaat ccgtaagagc gcatccgttc tttacggata ttatttaaaa tatctgggtt
    22381 attcgttagt cccacgattt ttttaggatc gatttcccac agctgtttgg gaatatgtgc
    22441 ttggggaatc aaaggtaaat tagcaacttt taaattttta tttgctaaaa acaagctcaa
    22501 aggcgttttg gaagttcggg atacgcctag caaaacgaca tctgcttcta aaaaaccacg
    22561 aggatctttc ccatcatcgt attttacagc aaattccatt gctttgatcc gtttgaaata
    22621 gttctcattc aataaatgga gggcaccagg ttgccgaatt ggcttgatac cagttaaacg
    22681 ttcaatttct gatatcggtg gcgtcatcac atcgaaacta aataattgat gttcttcaca
    22741 aaaagaacgt gttatctcga tcagttcttg attgattaat gtatgcagga caagtgcttt
    22801 ttcttctaat gcatcttgca atgcctgcct taatgttttt tcatctgtca cgaacgttct
    22861 gcggaatagg ttgaactcaa cggtaggata ctgagccata gtagcttgcg ctagttttgt
    22921 cgcagtttct ccagcagagt cggagatgac aaaaaaagta acgatttctg tagaatcgga
    22981 agacatggat aaaacctcac ttgcattttt tttatgttta ttataccata ctctagaatg
    23041 cgtacttcgt aatcattttg atttggagga ataagtcatg aaaaaacaga cattgatcga
    23101 acaatcatta gaaacattga aaaataacgg ttttaaatat actaaaaaaa gagaagcatt
    23161 gttaacttat ttagtgaagc gtaaccgcta cgtatctgct aaagaagttt ttgactatat
    23221 gaatcaggaa ttcaaagggg taagttatga tacgatttac cggaatctcc atgattttgc
    23281 agagatcgat ttactggaag aaaccgattt aaatggtgaa atgaagtttc gttttcattg
    23341 ctgtcagggg gaaatcggcc atcaccatca ttttatctgt acagtgtgcg gaacaacgaa
    23401 agaaatccat atgtgtccaa tgaattattt cgaagaacag ctaggtggat gcacgatcga
    23461 agggcataga ttcgagattt tcggaagatg tgaaaattgt caataaggat ttaaataaaa
    23521 taacatttga taatttaaaa aaaaagcaag atttccttga cggtcaaaac agcattagat
    23581 ataatattta caggacagtc ttgttttttg agatatattt caataacaat tacggttact
    23641 ttttaagctc ggagggaggg aattaacatg tcaaaaacag tcgttcgcaa aaacgaatca
    23701 cttgacgatg ctcttcgtcg cttcaaacgt tccgtgtcaa aagcgggtac tttacaagaa
    23761 tctcgtaaac gtgaattcta cgaaaaacca agtgtaagac gcaagaaaaa atctgaagca
    23821 gctagaaaac gcaaaaaatt ctagttctgt gatgaattgg agttggtagt gtgtcactac
    23881 taagtagatt aaacgatgac atgaaagcag cgatgaaagc gaaagataag gaatctttac
    23941 aagtcatccg aatgatcaag tcatctatac aaaacgagca gattaaagaa ggtcacgact
    24001 tgacagaaga agaagaacta acggtgttgt ctcgcgaaat gaaacagcga cgtgattctt
    24061 tgcatgagtt tgaagaagca ggacgtgacg atttagctga aaaagtaaaa agtgaaattg
    24121 taatagtcga aaaatatatg ccggaacaac tttccgacga agaaattcga caacttgttc
    24181 aagaagcaat cactcaaact ggtgcttcat caatgaaaga atttggcaaa gttatgggtg
    24241 caattatgcc taaagtcaaa ggaaaagcag atggaaacca agtgaatgcg attgtgaaag
    24301 aactattaca atcttaattt tatcatttaa actgcggaca gtgattgtcc gcagtttttt
    24361 gtgttcgtaa aaaaagaact aaaaaacagc tagaagaaag aataagattc tttcttctag
    24421 ctgttttttt cctgtttaat atctcttatg ttaaattgtg tttgatttgg ttattcttct
    24481 ggtagttcat ttgcttcttg tttataccct gataggcgat aggcgtaata aaagagtaag
    24541 ctgccagcgt aaccaagtaa cgttgggacc atccagccaa gtcctaatgc agcaaaagga
    24601 acgtgatgat gataaaatcc tagtaagctt gctacgacag tttgctgact cactaatgca
    24661 ggcgaatttg ccaacatatc caatacggct ggaacggcag aaaagaacat cgttacttga
    24721 tagacaaatg gactcttttg gaagaatttt gctgttaagg aaagcaagat taacgcaagt
    24781 gacaatggat acaaaagcat caatactggt aaagaccatt ggatgatatt atccaatccg
    24841 gcattcgcaa cgacaaaaga gacaaaagtc gttaaccgca gccatgtcat ataactgact
    24901 ttagggaaca gtttatggaa atcttgtgca aaagaaacaa cgagtcccat agcggtagtg
    24961 aagacaccta atgtgaccat tacccctaag aagctagcac cgaagttacc taaataaaaa
    25021 ttagtgatct gagaaagtgc agttccgcca ttttctgata aaggcatctt attcaaactc
    25081 atcgcaccga gaaggaccag accaaaataa atcatgactt ctaacgcaac gctcaggcta
    25141 ccagcttttg ctgttaattc tgtgactttt ttatctttaa aaccaagatc acgaactgca
    25201 tggacaaaag tgacacttaa agcaagtaaa gctactgcat caaccgtgtt gtatccttct
    25261 aaaacgccat ttgtcaaagc gttcgtctga taagaatctg cagcagattg gtttagattc
    25321 cccatgggtt ggaataaaga aacgacaaaa atgatagcta ataatgcaag aaacaaggag
    25381 tttaaatact ttccgacata tttgaccaaa cgtgttggat gtacagtaag gaaataagca
    25441 agtaaaaaga aaagagcaga gaataaaaac atacctaatg aatcatattt ttcaggcaaa
    25501 aagggtttag cagccatttc aaaagcagtt gctgctgtcc gcggtgtacc aaagaatggt
    25561 ccgatcgtta aatgaacaag gatcaagaac aaggcagcat accattttcc gacaggttta
    25621 gctaagtcat acagaccatc actttttgtc actactacag ctaagatagc taataatggg
    25681 aaaagagcgc ctgagatggc aaatcctaca ccagcgctga tccagtttga tccagccatt
    25741 tgacctaagt ggacaggaaa gatcagatta cctgacccga agaacatccc gaagataagt
    25801 gaactgacca tcaataattg cttccatgaa taagtttttg tttcttccat aaatttttcc
    25861 tccaataatt atcattttga gtaacaaaat aaacgttgca gaaactagaa atgccactaa
    25921 tatcagattc catgaaaacc accaccttaa aatataaaaa aaagcccttt tccacttggg
    25981 aaaagggctc tagattctag aacggtacca ccttttttcg cttaaaaaag cttcttcatt
    26041 cacggacgct tttcgataaa gttatccgct agcctgataa tggaggcagc cacaatgatc
    26101 tacgttttcg acattgactc ggaaatgatg ttcgtctatt cgatttattg tcatttctca
    26161 cttacatgac tcactgggaa taaatcgctg aatagcctac tgttttcctc gacgctttgc
    26221 tgtatattta tttgttgacg ttataataca cctgtttttt ttgaaatgca agaatatttc
    26281 aaatattttg ttttcttttt ttgaaacaag aaaaataccc agagaatact tgctaagtta
    26341 gacggaatac ttgtcctcta tcttatgcat agcttacatg atcagctttt tttattcaaa
    26401 cgagaccttt agagaatgga agcagctatt ttttaacgtt tcttggtact tatggttaag
    26461 caactctttt agaacctttt cttatttgct atatagtttc ttctgagtat ggtaaaatag
    26521 gaaagaatga atcgaaaaaa aggggatgtc tatttttgac ggaagagtct agttctttag
    26581 agattagatt gacggctgct gatgatgtca gcatgctttt aggtgcacac gacaagcata
    26641 taaaactgat tgaagaaaca acagaaacaa ccatccatac acgtggagaa atcattcaga
    26701 tcattggcga acaatcccag atttccttgg ctgcctcagt cattcggaca ttgcaggaat
    26761 tgatcaaacg aggaatccat gtaagtacac cagatgtcgt gacggcactg aaaatggggc
    26821 aaaaaggcac attagattat tttgttgaaa tgtatgagga agagatcgtc aaagaccgta
    26881 atggaaaacc aatacgggta aaaaacagcg gccaaaagaa atatgtcgaa tcggtagcca
    26941 aacatgatat cgtatttggc gtaggaccag ccgggacagg aaaaactttt cttgcagtag
    27001 tattagcaat cgctgccttg aaaaaaggtg aagtgcaaaa aatcattttg acgcggcctg
    27061 ctgtggaagc aggggaaaat ctaggctttc ttcccgggga tctaaaagaa aaagtagatc
    27121 cttatctgcg tcctgtttat gatgctttat accaaatttt tggattagat catacgaacc
    27181 gattgatgga acgaggcgtg atcgaaattg ctcctttagc ttacatgcgg gggcggacat
    27241 tggatgatgc attcgttatt ttggatgaag cacagaacac gacagttgct cagatgaaaa
    27301 tgttcctgac acgtcttggt tatcaatcaa aaatgattgt caatggagat acgagtcaga
    27361 ttgatctgcc tcgagggaca acaagtggtc ttgttcatgc agaacgaaca ttaaagcaaa
    27421 tcaagaaaat cgattttgtt aattttgaag caagtgacgt tgttcggcac cctgtagttg
    27481 ccgaaatcat caaagcttat gaaaaggcag atctccatca agaataagct ttgcttagga
    27541 ggataagcac atgttgaata aaacactgca gaaactacaa gaaaaacttg gaaaggcagc
    27601 attgcctttc ctgctgttct tgttttttat cattatctgc ggcgttacat ttagtagtgt
    27661 caaacaaaag gccaatgatt tcagagaagg ccaagtagca gaagaaagta tccgtgcgaa
    27721 caaaacgatc gagaacacgg aagaaaccga acaaaaaaga aaactggcag cagaagcagt
    27781 cactccagaa tacacttacc aaaaagatct tgcagatgac cagaacaatc ggatcaagca
    27841 actatttgaa ttgatcgata aaacgaatga tgccatcaac aaatcttatg acgaaaagat
    27901 tgataaggca aaggaagatg aaaatatccc aaagcctact acagaagagc ggatcgccgc
    27961 gttgaagaaa aattttgaaa atgtgaatgc agaaaatgtt gcgttttatc aaaaactacc
    28021 gaacaatttt tattcgacga tatttgaaat gactgaaact gagatccata ctgtgaggga
    28081 tgagagtctg aagctcattg acgaacagat gagcaaacaa gttcgtgaaa gtgaattgga
    28141 agcttttaaa caagaagcag aagagcaaat ccaatatttg aatgtcacac cagcacaaca
    28201 acaaatgatt cgctacttag tggatcaagg tatcgtagtg aatgacgtat tgaatgagaa
    28261 aaagacagaa gaattgaaac aatccgcacg agaagctgta caaccagtga tgatcttcca
    28321 aggagaaatc attgttcgtg aaggtaatca aatcgatgct tcagctatga aaaaattgga
    28381 attactagga ctgactaatc aaacaacttc gatttttccg ctaattgcca tggttttagc
    28441 tgttttgctt caaatcggcg tactgattta taattcactt caattccaaa atgcaggaga
    28501 aagaacaaaa tacgtgcttt tctatgtgac agctatgtca gtcagcatag tgctgatgaa
    28561 atttttccaa ttattccaaa cggaacaagc tacttatatc ccgctgtttt atcccgctgc
    28621 ttttgcacca cttgttttaa gctttttctt gaaccgaaga gcagggatca tggctggtat
    28681 tttccaagca gtatctgcac ttttcatttt ttatgattcg attggcacga actttctaac
    28741 gattatcttg atgtcttatg ttttctctgg gctcatggca acgattgtca gacgcaaacg
    28801 gatctcagaa caaggattat ctgctgccat gtgggtcatt atttttcctg tgatgatgga
    28861 tgtcattttg atcgtctatc aaggaatgag ctttggtgat gcaacgactt ggttaatgct
    28921 tgtctgcggg ttcactggtg cactcttctc attcttgctg acgatcggtc tacacccata
    28981 tattgaattg atggtcaatg atgacagtgt aattgtatta aatgaactca gcaatccaaa
    29041 tcatccttta ttgaaacagt tgctggaaga agcgccaggt acttatcatc atagtatgat
    29101 ggtagctaat ctaagtgcta acgctgtagc agaaatcggc ggacgttctt tgctaactcg
    29161 agtagcttgc tattatcatg atatcggcaa gatcaaacat gctagctttt tcgttgagaa
    29221 cttgccttca ggagcggaaa acccgcacaa tttcttattg ccagaagaca gtaaacatat
    29281 tatctttgga catgtgacag atggagccaa aatattggaa gattataata tgccgcaaat
    29341 ggtgatcgat atttgcaaac agcatcatgg aaccacgcta atgcgttatt tctacatcaa
    29401 agcgaaagaa cgcaatccgg aagtaacaga agagcaattc cgttatcctg gaccaaaacc
    29461 gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt tgtgaggctg cagtacgggc
    29521 aatggatcat ccgacgagcg aaaaaatcga aagttttgtc cataatctta tcgaagagcg
    29581 tatttccgat ggtcaattag acgatagtgg actgaccttg aaagaaatcc gaaaagtgga
    29641 aaaatcattg atcagcggtt taagtagcac cttccattct cggatcaaat atcctaaaat
    29701 gaaaacagaa gttgagaaga taaaggaaga acaagaagaa tagaaagaag cgatgtataa
    29761 tggatattac ctttatggat gaaacaaacc aagtctcaga agagaaaatg aaagaaatcg
    29821 atgacttgct gcaatttgca gcatcttatt taaagctgcc agaggaaaca gaaatgtctg
    29881 ttactttcat ggacaatgcg gcaatccaag taatcaatcg tgattaccgt ggaaaagatg
    29941 caccgacaga cgtcatcagt tttgctctgg aagaagaagg cgaagacgaa atcccagtga
    30001 tttttgaaga tggagagaat ccattgcctc gtgtactagg agacatcatg atttctattg
    30061 aacgtgctgc tgaacaagca gaagcttacg gccattcata tgatcgggaa cttggctttt
    30121 tagcggtaca tggattcttg catatcaacg ggtatgacca tatgacacca gaagatgaga
    30181 aagaaatgtt tggtttacag aaagagatct tggatgccta tggactcaaa agataaaaac
    30241 gtagagaaaa acaaacattt cctgatgtct gtggaatttg ctgtccaagg agtcaaaacc
    30301 gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt tcggtatcct tgctttattt
    30361 atgggcgtga ttttccagct tgatcggata gagtggttat ggctgctttt agctgttttt
    30421 ctcgtttgga tagtagagat catcaatact gtttttgaaa atgtagtgga catgtttacg
    30481 gattttcatt ttcatccgat tgggaaaaaa atcaaagata tggcagctgg agcagtttta
    30541 gtgaccgctt gttttgctgt catcattggg ttgattttat ttgtccctaa aatatatcat
    30601 ttgttgttta attaaaaagg agaacgtata tgccagaaca taaatcaggg tttgtcgcaa
    30661 tcgtcggccg cccgaatgta ggaaaatcca cattattgaa ccgaatcgtg ggtcaaaaaa
    30721 ttgccattat gagtgacaag gcacagacaa ctagaaataa aatccaagga gtatatacta
    30781 caccagaagc tcagctgatt tttatcgata caccaggaat ccataaacca aagcatcgtc
    30841 taggagattt tatggtagaa gcagcttaca gtgcgttgaa agaagtggat gcagtcttat
    30901 ttatgatcag tgctgatcaa aaacgcggcc ggggagacga ttttattatt gaacgtctaa
    30961 aaaacgtgca ttctccagtt tatctgatta tcaataaaat cgataagatc catccagacg
    31021 agttgctggg aatcatcgaa gattattcga cgcagatgcc ttttgcacaa gtgattccga
    31081 tctctgctac agaaggaaac aatgtcgaac gcttaatgga tgtattagtc agtgaaatgc
    31141 cagagggccc acaatatttt ccagatgatc aagtgactga tcatcctgaa tactttatcg
    31201 tttctgaatt gatccgtgag aaagtcttgt ttttaacacg agacgaagtg cctcattctg
    31261 tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga taaaatccat atccaggcca
    31321 cgatcatcgt cgaaagagat agccaaaaag gaatcattat cggcaaaggc ggaaaaatgc
    31381 tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa tcttttagga gacaaagtat
    31441 ttctagaact ttgggtcaaa gtccaaaaag actggcgaga caaaaaagtc tatctgcaag
    31501 actttggcta ccgaaaagac gaatactgat attctgaact tcttataggt taagtataga
    31561 aataaactgg ataaaaatcc gcacgttgag aaaaacggcg gaagatcgaa acataaataa
    31621 tctggatcgg cagaatctgc ttttccagat ttatttttta ggagggagaa tatgagccag
    31681 cttgcagaat cgaaaggctt gattttatct agccgtaatt acaaagaaaa agataaatta
    31741 gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt atatcaaagg aatccatcgg
    31801 caaaatcagc cattagctcc ggctttgctc ccctttaccg aagcagtcta tatcggtaat
    31861 tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag acgtccatgc gtttcggaac
    31921 atccaagaag atatcttttt gagcggttat ggtacctatt tgctaaatct agtagatgca
    31981 gcaatcgaag atcatcaata tgacccgcat ttattccaat ttacgcaaca agctctgcag
    32041 cggatggatc aaggagatga tgcagaaatc atcacgaata tctttgaagt tcaaatactg
    32101 caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg tatgcgggga aacaaaagga
    32161 aaatttgact attcttccaa atctggcggt gtgatatgcg agaaacactg gccaatggac
    32221 gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg ttcgaatgtt ttcggctatt
    32281 tcttatgata agatcagtgg aatccacttg aaagaagaaa cgaagcaggc aatccgtgac
    32341 ctcattgacc agttgtatga tgaatatgtc ggtattcatc taaaaagcaa aaagttcatt
    32401 gatcaaatga agtcttgggc acacgtatta aagacgccag agaaaaatat gcctgaaaaa
    32461 gagatacctg aaaaagaaga ataaatgaaa gatacaacaa aaaaatcaaa actatttgac
    32521 aaatacaggg acttccgtct attattaacc gtagattaaa tagatcgttc actgagagga
    32581 tctattatat aaacaagaaa actgcaaaat gaatacgtac attgaccaag atcagtaaaa
    32641 aacacgtggt ttagcgagtt cgggagagtg gaagccggac ccaaacgatt tttgaagagg
    32701 cacttgcgag tacgataaat gaagctgccg aatctttatt cggaagtagg gtggaaccgc
    32761 gaaattattc gtccctatgt ctgtaatcag acatagggac tttttgtcgt tttctttcct
    32821 gtattcttat tgacagcaac gcttaaaaat tgaaaggggc aaaacagatg agtaaaaaat
    32881 tgaccgtcca agatatgatt ttaacattac aaaaattttg gtcatcaaac ggctgcatgc
    32941 tgatgcaagc ttatgacaca gaaaaaggag caggtacgat gagcccatat acattcttac
    33001 gggcaatcgg accggaacca tggaatgctg cttatgtaga gccatcccgc cgaccagctg
    33061 acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca tcatcaattt caagtcgtaa
    33121 tgaaaccttc acctgaaaat atccaagagc tttatttaga aagcttgcgt cttttaggaa
    33181 ttgatccatt agaacacgat attcgttttg ttgaagacaa ctgggagaac ccatcgatgg
    33241 ggtgtgccgg tgtgggttgg gaagtatggc ttgatgggat ggagatcaca caattcacgt
    33301 acttccagca agtcggagga ctaccttgta aaccagttac ttctgagatc acttatggac
    33361 tagaacgtct tgcttcatat attcaagaag ttgaaagtgt ctatgatctg gaatgggctg
    33421 atggtgtgaa atatggcgag atcttcaaac aaccagaata tgaacattcc aaatattctt
    33481 ttgaagtaag tgaccaagac ttgctactaa gcaactttga ccgttttgaa aaagaagcta
    33541 aacgctgtat cgatgaaaat cttgttcatc cagcgtatga ttatattttg aaatgcagcc
    33601 atacattcaa tttattagat gctcgcggtg cagtatccgt aacagaacga gcaggttatc
    33661 tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat ctttgtggca gaacgcgaaa
    33721 aattaggatt ccctttattg aataaaaaca cagcaaatcc agaaaacatg aaggaggctg
    33781 aataagatgg caaaagactt attacttgaa atcgggctgg aagaaatgcc tgctcatgtt
    33841 gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa aatttttaga tgaaaaccaa
    33901 ttgacttatg atacgatcga aacgttttct acaccgcgcc gcttagcaat caaagtaaca
    33961 aacattccag aacgtcaagc agacagtgaa gaagaagtca aaggaccagc caaaaaaatt
    34021 gctttggacg cagatggaaa ctggacgaaa gcagctgaag gatttgtccg cggacaaggt
    34081 ctgacgacag atgctatcac ttttagagaa ttaaaaggcg tggaatatgt ctatgtaacg
    34141 aaatacactc acggcaaaga agcgaaagaa gtattgacag gtttagcaga tgtcattaaa
    34201 agcctcactt tcccagtaac gatgcattgg gcagaatacg attttgaata tatccgccct
    34261 attcactgga tcgtggcatt gttagatgac gaagtaatcc cgtttgagat cttagatgtc
    34321 aaaacaggca gagcctctcg aggacatcgt ttcttaggcg aagatgtgac atttgcgcat
    34381 gcaaaagaat atgaagaaaa attgaatgaa caattcgtta ttgcttcacc gcaaaaaaga
    34441 caagaaatga tcgttacaca agcaaatgaa ttagccgaaa aaaatcactg ggttattgat
    34501 ctagacgaag atttactaga agaagtcaac aatttagtcg aatatccaac tgtcttttct
    34561 ggcgacttcg aagaaaaata tctttctgtt cctgaagaag tactagtcac ttctatgaaa
    34621 gaacatcaac gttatttcga ggtacggaac gatcaaggta tgctgcttcc tcattttatt
    34681 tctgctcgta atggtgatcg ggtaaaattg gaaaatgtgg ttcgaggcaa tcaaaaagta
    34741 ttgacggctc gtttagaaga tgcggaattc ttttataatg aagacaagaa agcttcaatc
    34801 gaccaatacg ttgaaaaact aaaaactgtt actttccatg aaaaaatcgg tagtatctat
    34861 gagaaaatgc agcgggtaca agtcattgcc cgtatcattg gcgaacaagt aggactatct
    34921 gaggaagaat tgatggatct gcaacgagct gctgaaatct ataaatttga tctagtaacc
    34981 aacatggtag gggaatttcc tgaactacaa gggatcatgg gcgaaaaata tgctctgctg
    35041 aaaggcgaga aacctgctgt agcaacagcg attcgtgagc attacatgcc tatatccagt
    35101 gaaggagagc tacctcagtc tacagtaggt tctgttttag cgattgcaga taaattagat
    35161 agcatttttt ctttctttgc agttgggatg attccaagcg ggtcaaacga tccttatgcc
    35221 ttacgtcgcc aagcatacgg tgtcgttcgg atcatcgaat cgaaaaaatg ggcctttcct
    35281 ttgtctgttc tgcaagaaac gattagtgat gttatcagta aagatacgga tcgtttcggc
    35341 attggtttat cagaagggca acaacaggtg atcgacttta tcaaaggccg tctgcgccaa
    35401 ttattgacaa cgaagaatat tcgtcatgat gtcatcgaag ctgttttgaa tgcagaacaa
    35461 aaagatctga caaaagtatt tgcagcagca caattgttca agcagcatct tgcagatgaa
    35521 gacttcaaac catcgatgga agctttgaca cgtgtcgtga acttagcgaa aaaagcggag
    35581 cttgaaaaac aatcagaagt agaccctgaa ctgttcgaaa atgaagcgga gaaagaactg
    35641 cacaaagcag tagaggcaat gaaaaaaagc tttgctgacc aaacgatcaa cgaaaattat
    35701 gatcagcttg taagccttcg cccactgatc gaaaattatt ttgatcagac gatggtcatg
    35761 gcagaagaac cagcagtacg taagaaccga ttaacgcaat taagtcaaat tgctgccatg
    35821 gctttgtcgc tagcaagttt agatcaaatc atcacaaaat aatttctaat atgtaaataa
    35881 accagagaat aggcacaaaa
//
LOCUS       DAYYDS010000020        34869 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000020 DAYYDS010000000
VERSION     DAYYDS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.020"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACHA02_000975"
     CDS             complement(59..187)
                     /locus_tag="ACHA02_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8669341.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACHA02_000976"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACHA02_000976"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8669342.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACHA02_000977"
     CDS             complement(1674..2729)
                     /locus_tag="ACHA02_000977"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8669343.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACHA02_000978"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACHA02_000978"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8669344.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACHA02_000979"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACHA02_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8669345.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACHA02_000980"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACHA02_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8669346.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACHA02_000981"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACHA02_000981"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8669347.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACHA02_000982"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACHA02_000982"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8669348.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACHA02_000983"
     CDS             10491..12050
                     /locus_tag="ACHA02_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8669349.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACHA02_000984"
     CDS             complement(12161..14092)
                     /locus_tag="ACHA02_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8669350.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACHA02_000985"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACHA02_000985"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8669351.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACHA02_000986"
     CDS             complement(15021..15773)
                     /locus_tag="ACHA02_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8669352.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACHA02_000987"
     CDS             complement(15932..16141)
                     /locus_tag="ACHA02_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669353.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACHA02_000988"
     CDS             complement(16152..17480)
                     /locus_tag="ACHA02_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669354.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACHA02_000989"
     CDS             17616..17771
                     /locus_tag="ACHA02_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8669355.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACHA02_000990"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACHA02_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8669356.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACHA02_000991"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACHA02_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8669357.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACHA02_000992"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACHA02_000992"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8669358.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACHA02_000993"
     CDS             complement(21455..22387)
                     /locus_tag="ACHA02_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669359.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACHA02_000994"
     CDS             complement(22544..23203)
                     /locus_tag="ACHA02_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8669360.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACHA02_000995"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACHA02_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8669361.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACHA02_000996"
     CDS             complement(24244..24534)
                     /locus_tag="ACHA02_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669362.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="ACHA02_000997"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="ACHA02_000997"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8669363.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /gene="glmM"
                     /locus_tag="ACHA02_000998"
     CDS             complement(26820..28175)
                     /gene="glmM"
                     /locus_tag="ACHA02_000998"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8669364.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="ACHA02_000999"
     CDS             complement(28199..29368)
                     /locus_tag="ACHA02_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8669365.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /gene="cdaA"
                     /locus_tag="ACHA02_001000"
     CDS             complement(29365..30252)
                     /gene="cdaA"
                     /locus_tag="ACHA02_001000"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8669366.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="ACHA02_001001"
     CDS             complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="ACHA02_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8669367.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34332..34853
                     /locus_tag="ACHA02_001002"
     CDS             34332..34853
                     /locus_tag="ACHA02_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8669368.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         9400 a         7569 c         6284 g        11616 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc
    30541 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    30601 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    30661 tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag
    30721 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    30781 tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac
    30841 caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc
    30901 aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct
    30961 gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca
    31021 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    31081 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    31141 ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca
    31201 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    31261 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat
    31321 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca
    31381 aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    31441 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    31501 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    31561 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    31621 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    31681 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    31741 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    31801 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    31861 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    31921 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    31981 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    32041 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    32101 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    32161 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    32221 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    32281 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    32341 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    32401 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    32461 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    32521 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    32581 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    32641 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    32701 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    32761 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    32821 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    32881 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    32941 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    33001 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    33061 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    33121 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    33181 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    33241 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    33301 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    33361 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    33421 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    33481 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    33541 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    33601 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa
    33661 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    33721 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    33781 tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc
    33841 ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt
    33901 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    33961 actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact
    34021 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    34081 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    34141 catccttgtt catattctct cttattatct gttccattat aacgtaaaac atgccatttt
    34201 tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta
    34261 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    34321 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    34381 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
    34441 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    34501 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
    34561 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
    34621 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    34681 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    34741 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
    34801 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    34861 aggagcgtg
//
LOCUS       DAYYDS010000021        34636 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000021 DAYYDS010000000
VERSION     DAYYDS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.021"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACHA02_001003"
     CDS             591..1280
                     /locus_tag="ACHA02_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8669369.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACHA02_001004"
     CDS             1267..2475
                     /locus_tag="ACHA02_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8669370.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACHA02_001005"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACHA02_001005"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8669371.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHA02_001006"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHA02_001006"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8669372.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACHA02_001007"
     CDS             complement(6578..7273)
                     /locus_tag="ACHA02_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8669373.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHA02_001008"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHA02_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8669374.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACHA02_001009"
     CDS             complement(8472..8663)
                     /locus_tag="ACHA02_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8669375.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACHA02_001010"
     CDS             complement(8684..9574)
                     /locus_tag="ACHA02_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8669376.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACHA02_001011"
     CDS             complement(9561..10328)
                     /locus_tag="ACHA02_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8669377.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHA02_001012"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHA02_001012"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8669378.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHA02_001013"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHA02_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8669379.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHA02_001014"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHA02_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8669380.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACHA02_001015"
     CDS             14899..15282
                     /locus_tag="ACHA02_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669381.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHA02_001016"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHA02_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8669382.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACHA02_001017"
     CDS             complement(16226..17044)
                     /locus_tag="ACHA02_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8669383.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHA02_001018"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHA02_001018"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8669384.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHA02_001019"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHA02_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8669385.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHA02_001020"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHA02_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8669386.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHA02_001021"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHA02_001021"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8669387.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHA02_001022"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHA02_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8669388.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ACHA02_001023"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ACHA02_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8669389.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHA02_001024"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHA02_001024"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8669390.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHA02_001025"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHA02_001025"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8669391.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHA02_001026"
     CDS             27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHA02_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8669392.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ACHA02_001027"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ACHA02_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8669393.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHA02_001028"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHA02_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8669394.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ACHA02_001029"
     CDS             28352..30325
                     /locus_tag="ACHA02_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8669395.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ACHA02_001030"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ACHA02_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8669396.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHA02_001031"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHA02_001031"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8669397.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ACHA02_001032"
     CDS             33204..34496
                     /locus_tag="ACHA02_001032"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8669398.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DAYYDS010000022        34330 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000022 DAYYDS010000000
VERSION     DAYYDS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.022"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1077
                     /locus_tag="ACHA02_001033"
     CDS             202..1077
                     /locus_tag="ACHA02_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8669399.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ACHA02_001034"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ACHA02_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="ACHA02_001035"
     CDS             complement(1642..2973)
                     /locus_tag="ACHA02_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8669400.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="ACHA02_001036"
     CDS             complement(3122..3835)
                     /locus_tag="ACHA02_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8669401.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="ACHA02_001037"
     CDS             4010..5434
                     /locus_tag="ACHA02_001037"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8669402.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="ACHA02_001038"
     CDS             5733..6440
                     /locus_tag="ACHA02_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8669403.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="ACHA02_001039"
     CDS             6427..6750
                     /locus_tag="ACHA02_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8669404.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="ACHA02_001040"
     CDS             complement(6839..7876)
                     /locus_tag="ACHA02_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8669405.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="ACHA02_001041"
     CDS             complement(8197..9180)
                     /locus_tag="ACHA02_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8669406.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="ACHA02_001042"
     CDS             complement(9595..10365)
                     /locus_tag="ACHA02_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8669407.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="ACHA02_001043"
     CDS             complement(10358..11293)
                     /locus_tag="ACHA02_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669408.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="ACHA02_001044"
     CDS             complement(11609..13258)
                     /locus_tag="ACHA02_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8669409.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="ACHA02_001045"
     CDS             complement(13375..15978)
                     /locus_tag="ACHA02_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8669410.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="ACHA02_001046"
     CDS             complement(15990..17039)
                     /locus_tag="ACHA02_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8669411.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="ACHA02_001047"
     CDS             complement(17065..17379)
                     /locus_tag="ACHA02_001047"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8669412.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="ACHA02_001048"
     CDS             complement(17395..17841)
                     /locus_tag="ACHA02_001048"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8669413.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="ACHA02_001049"
     CDS             complement(17842..19764)
                     /locus_tag="ACHA02_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8669414.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="ACHA02_001050"
     CDS             complement(19976..20872)
                     /locus_tag="ACHA02_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8669415.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="ACHA02_001051"
     CDS             complement(20865..21545)
                     /locus_tag="ACHA02_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8669416.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="ACHA02_001052"
     CDS             complement(21546..22328)
                     /locus_tag="ACHA02_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8669417.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="ACHA02_001053"
     CDS             complement(22321..23238)
                     /locus_tag="ACHA02_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8669418.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23656)
                     /locus_tag="ACHA02_001054"
     CDS             complement(23402..23656)
                     /locus_tag="ACHA02_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669419.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="ACHA02_001055"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="ACHA02_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8669420.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="ACHA02_001056"
     CDS             27853..28167
                     /locus_tag="ACHA02_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8669421.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="ACHA02_001057"
     CDS             28180..28554
                     /locus_tag="ACHA02_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8669422.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="ACHA02_001058"
     CDS             28564..28899
                     /locus_tag="ACHA02_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8669423.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="ACHA02_001059"
     CDS             28982..30322
                     /locus_tag="ACHA02_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8669424.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="ACHA02_001060"
     CDS             30400..31074
                     /locus_tag="ACHA02_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8669425.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="ACHA02_001061"
     CDS             31067..31231
                     /locus_tag="ACHA02_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669426.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            31307..32449
                     /gene="dcm"
                     /locus_tag="ACHA02_001062"
                     /pseudo
     CDS             31307..32449
                     /gene="dcm"
                     /locus_tag="ACHA02_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            32439..32729
                     /locus_tag="ACHA02_001063"
     CDS             32439..32729
                     /locus_tag="ACHA02_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669427.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /gene="mobT"
                     /locus_tag="ACHA02_001064"
     CDS             32988..34172
                     /gene="mobT"
                     /locus_tag="ACHA02_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8669428.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34169..34306
                     /locus_tag="ACHA02_001065"
     CDS             34169..34306
                     /locus_tag="ACHA02_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8669429.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT        10701 a         6609 c         6254 g        10766 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DAYYDS010000023        33737 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000023 DAYYDS010000000
VERSION     DAYYDS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.023"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..498
                     /locus_tag="ACHA02_001066"
     CDS             214..498
                     /locus_tag="ACHA02_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8669430.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="ACHA02_001067"
     CDS             complement(779..1198)
                     /locus_tag="ACHA02_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8669431.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(1200..1784)
                     /locus_tag="ACHA02_001068"
     CDS             complement(1200..1784)
                     /locus_tag="ACHA02_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8669432.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ACHA02_001069"
     CDS             complement(1831..3306)
                     /locus_tag="ACHA02_001069"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8669433.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ACHA02_001070"
     CDS             3487..3798
                     /locus_tag="ACHA02_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669434.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="ACHA02_001071"
     CDS             complement(3913..6249)
                     /locus_tag="ACHA02_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8669435.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="ACHA02_001072"
     CDS             6391..7059
                     /locus_tag="ACHA02_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8669436.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="ACHA02_001073"
     CDS             7062..8105
                     /locus_tag="ACHA02_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8669437.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="ACHA02_001074"
     CDS             complement(8342..9223)
                     /locus_tag="ACHA02_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8669438.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="ACHA02_001075"
     CDS             9382..10110
                     /locus_tag="ACHA02_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8669439.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="ACHA02_001076"
     CDS             10266..10583
                     /locus_tag="ACHA02_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8669440.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="ACHA02_001077"
     CDS             10576..11184
                     /locus_tag="ACHA02_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8669441.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="ACHA02_001078"
     CDS             11239..12030
                     /locus_tag="ACHA02_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8669442.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12680
                     /locus_tag="ACHA02_001079"
     CDS             12168..12680
                     /locus_tag="ACHA02_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8669443.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12706..13578
                     /locus_tag="ACHA02_001080"
     CDS             12706..13578
                     /locus_tag="ACHA02_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8669444.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="ACHA02_001081"
     CDS             13754..14116
                     /locus_tag="ACHA02_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8669445.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="ACHA02_001082"
     CDS             14125..14877
                     /locus_tag="ACHA02_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669446.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="ACHA02_001083"
     CDS             14891..16693
                     /locus_tag="ACHA02_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8669447.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18010
                     /locus_tag="ACHA02_001084"
     CDS             16970..18010
                     /locus_tag="ACHA02_001084"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8669448.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18062..20206)
                     /locus_tag="ACHA02_001085"
     CDS             complement(18062..20206)
                     /locus_tag="ACHA02_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8669449.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20563..21936)
                     /locus_tag="ACHA02_001086"
     CDS             complement(20563..21936)
                     /locus_tag="ACHA02_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8669450.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="ACHA02_001087"
     CDS             complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="ACHA02_001087"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8669451.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="ACHA02_001088"
     CDS             complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="ACHA02_001088"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8669452.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23384..24397)
                     /locus_tag="ACHA02_001089"
     CDS             complement(23384..24397)
                     /locus_tag="ACHA02_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8669453.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24478..25077)
                     /locus_tag="ACHA02_001090"
     CDS             complement(24478..25077)
                     /locus_tag="ACHA02_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8669454.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25263..25964
                     /locus_tag="ACHA02_001091"
     CDS             25263..25964
                     /locus_tag="ACHA02_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669455.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25974..28685
                     /locus_tag="ACHA02_001092"
     CDS             25974..28685
                     /locus_tag="ACHA02_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8669456.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29082..30710
                     /gene="treC"
                     /locus_tag="ACHA02_001093"
     CDS             29082..30710
                     /gene="treC"
                     /locus_tag="ACHA02_001093"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8669457.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30770..31951)
                     /locus_tag="ACHA02_001094"
     CDS             complement(30770..31951)
                     /locus_tag="ACHA02_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8669458.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32203..33726
                     /locus_tag="ACHA02_001095"
     CDS             32203..33726
                     /locus_tag="ACHA02_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8669459.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT        10691 a         6050 c         6863 g        10133 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cgacaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tatgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatgtttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattc caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcactcatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgaa ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg atccgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atctgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gtttttttgc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattggac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaattt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 cttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tctgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aaggggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg tatgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaagtc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagatt tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttccggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cttggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctgg gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgatt ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcaagtctt tggtataggg gtagccttag ttacgatcat
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    17281 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    17341 ggtcattttc tatcagacta gcccagatat gggaaacgga tttcctggaa ctttagatgt
    17401 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    17461 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    17521 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    17581 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    17641 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    17701 cggcatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    17761 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    17821 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    17881 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    17941 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    18001 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt tttaatttat
    18061 tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac aaggttagta
    18121 caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt cttgccgtaa
    18181 aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg ttgttgacgc
    18241 gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc catttgattg
    18301 gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca gttgttgctg
    18361 cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt
    18421 tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt gcttctttgt
    18481 agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca gtcagtccat
    18541 ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat ttacggaatt
    18601 gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg aaggtcacgc
    18661 tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg cttgataata
    18721 agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat gcgtagattc
    18781 cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg
    18841 tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat gagccgcctg
    18901 tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca aaatcaggcg
    18961 tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca tggataaatt
    19021 gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt ccccagaaga
    19081 cgagatcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat tgctgttcgc
    19141 ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa gtcaaaccag
    19201 aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca agatgcttga
    19261 atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat acagcatcaa
    19321 ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata ccgctttgat
    19381 ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa ccattgtttg
    19441 gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg tttgtttgat
    19501 tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt gaaccattat
    19561 tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt gtactaggga
    19621 cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct ttaaattctg
    19681 cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa tttacttctt
    19741 tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta atagtgtaag
    19801 ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata tccaaaggtt
    19861 gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc aaagaaggag
    19921 caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca gccggatcaa
    19981 tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg tcttctgtaa
    20041 aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt gtgactgttt
    20101 gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca gatggagcaa
    20161 tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata aactagtcct
    20221 tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt ggaacagata
    20281 atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa taaaacaatt
    20341 tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat tgcatagacc
    20401 acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt aaattatacc
    20461 aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag aaaaagactg
    20521 aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat aagacattag
    20581 gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac taacagccag
    20641 tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg tcagactacc
    20701 aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac cagcagctgc
    20761 accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt aaacagttgg
    20821 ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt
    20881 tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa aattggctgc
    20941 tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga tcatgacagg
    21001 gtttacagcc cattggatac caaatattac caaaatattc caaccaccag cgatgattgc
    21061 gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta aaccttcgcc
    21121 aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga taactaatga
    21181 aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga ggaatttttc
    21241 aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg tatactgcat
    21301 cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg aaagtaaaga
    21361 ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc cattcacttt
    21421 aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga cggcattcga
    21481 cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa tcgtagtcgc
    21541 taaaacaacc agacctttta atataccaga accggcaaac agcggaatga tcggcgtaaa
    21601 gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt tcggagctga
    21661 atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat ccatgattat
    21721 ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct gttttaattt
    21781 ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg cacatggaag
    21841 gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga
    21901 cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc actcactaat
    21961 ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca gttattcaac
    22021 tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa caataatttg
    22081 attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc aatcgattgt
    22141 acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag accgtttgag
    22201 acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc cccgatactt
    22261 aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt gattactttt
    22321 ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga ttgttctaaa
    22381 gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac agaagaggct
    22441 agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag tgtgctgctt
    22501 aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac tttaaaagca
    22561 gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg
    22621 attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa actgttatga
    22681 tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat aatgatatct
    22741 gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt
    22801 gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa gctactttca
    22861 aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag gacttcttgt
    22921 tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc tgctgcgtct
    22981 accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc agatgtatcg
    23041 atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc cccaacgcct
    23101 ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta
    23161 cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg
    23221 gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt gttcatttta
    23281 caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa cgttttagta
    23341 aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc aagggtgcag
    23401 ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct ttttctggga
    23461 attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag attggttgtg
    23521 cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca tagccaataa
    23581 tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat aatccaaaag
    23641 ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt tctgctgtga
    23701 tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg tcgtgaggga
    23761 tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca ttttccggtg
    23821 gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag tgcatgcccg
    23881 ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct tcagattttt
    23941 tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg attgcatctg
    24001 tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg agctcggcta
    24061 gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg acgaaattct
    24121 gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga ttcgtgatat
    24181 caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta gcaaaatagt
    24241 caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt ttcggactga
    24301 atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg ccggaaagtt
    24361 tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta tctaaatcct
    24421 gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta
    24481 tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg cagttttcgg
    24541 aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc aagcatcttc
    24601 agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt
    24661 tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa gttgaagatc
    24721 aagagaagta ccaaaaaaag cacttggatt gtattttttg atagtcaaca tattatctgt
    24781 atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag aagcagtcat
    24841 tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta cgttcactaa
    24901 aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt ttgtcagaat
    24961 agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt gcatgtcgat
    25021 cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat cattcatgat
    25081 gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg tttatttgtc
    25141 aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa ataaataagt
    25201 gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat ggaggaatca
    25261 gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga gaaacgacca
    25321 ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg attatacttg
    25381 gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg gatacacctg
    25441 atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat aaacagctta
    25501 ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc gttcctaatc
    25561 taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg cttgatcctg
    25621 tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg tcccaattat
    25681 caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat cccaaacttc
    25741 ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat
    25801 tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca cataattctg
    25861 ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta aggtctgttg
    25921 aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat tttatgaaaa
    25981 caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga cgattcattg
    26041 caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa actgctggac
    26101 ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct gatttgcaaa
    26161 tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag atcccagatg
    26221 caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac gaagtgattc
    26281 aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact gatggaaaac
    26341 tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa
    26401 tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat accatcacgg
    26461 gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg aacgtcggaa
    26521 atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca gatatttatt
    26581 ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc aaagcttaca
    26641 aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat cgacccgaag
    26701 aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca cagcaaaaaa
    26761 tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat gctaaaaaac
    26821 agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga acaatcgctg
    26881 ttcaaacatt acctcaacaa gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga
    26941 aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa
    27001 atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt tttcaagaat
    27061 ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt ttctttttct
    27121 ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg
    27181 aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa
    27241 tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta ggaacgaatc
    27301 ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg agtgcgctta
    27361 ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt gccgcatttg
    27421 gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca ttgcttcaac
    27481 cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg ctgtggtcac
    27541 gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag tcaagaatgc
    27601 ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc gtgggattga
    27661 aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac tatcaagcaa
    27721 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
    27781 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
    27841 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
    27901 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
    27961 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
    28021 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
    28081 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
    28141 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
    28201 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
    28261 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
    28321 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
    28381 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
    28441 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
    28501 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
    28561 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
    28621 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
    28681 aataaaaaca gaggttgtga aatcagcgtt ttgagctaat aacgagggtc agctgctaat
    28741 tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc
    28801 aaataaaatt agctcctcat gttttacatt tgagaagact gattgccgta gataaaagct
    28861 tttgaacacc atttatagaa gaaacaagaa tattttgcta ttttaaacaa gaggatagac
    28921 aaatacatgt ctgatctctt gttttttatt gggcaacaga cctcttagaa tgtataaaga
    28981 taaattttac aaacgctttc tgttcagtta taataataaa tgataacttg tatatataag
    29041 ctgtggtagg taataaatca gttgaatgga ggtaaatatc catgacattt aaagacaaag
    29101 tcatttacca aatctatccc aaatcttaca aagacagtaa tggtgatgga attggagatt
    29161 taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct aggcatcgac atgatttggc
    29221 taaaccctat ttatccaagc cctcaaaaag acaacggtta tgatatctct gattacacag
    29281 ccatcgatcc tatctttggt acgatggagg aattcgaaca gctaatagcc gaagcaaaga
    29341 aataccggat agaaatcatg ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat
    29401 ggttcaaaaa agcactagca ggggaccagc attatcagga cttcttcatt ttaagagaag
    29461 agccaacgga ttgggtatca aaatttggtg gaagtgcttg gtcgccattt ggagatacgg
    29521 ggaaatatta tttgcactta tatgatcgga cacaagctga cttgaactgg cggaaccaag
    29581 cagtaagaga ggaactattc aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat
    29641 tccgtttcga tgttatcaat gttatcggta aagacgaaca attgaaaaat aataaagaaa
    29701 atgatggcaa acctgaatat acggacaaac caatcaccca tacgtatcta aaagaattga
    29761 accaagcaac gtttgggacg gattccacta tcatcacagt cggtgaaatg agctctacga
    29821 cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga agaattatca atggtcttta
    29881 atttccatca tttgaaagtc gattatgaaa atgggaataa atggtctact cctccatttg
    29941 attttgcaga attaaagcga ctatttcata catggggaga aaatatgagt gagggtaacg
    30001 gctggaatgc tctatttttt aataatcatg atcagccacg tgcgctcaat cgatttgtag
    30061 ttatcgatca gtatcgaaaa caaggagcag agatgctagc aaccatgatc catttgaacc
    30121 gtgggacgcc ctatatttat atgggagaag aaatcggcat gatcgaccca gactattcat
    30181 cgatttccga ctatattgac gtagaaagtt tgaatgctta taagttactc cttgaacaag
    30241 gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa
    30301 cacccatgca atggtcagac acagaacaag caggatttac tacagggaaa ccttggttaa
    30361 aagttggagg caagctagaa gagattaatg tagaaaaaga actatcttct gaagattcta
    30421 ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac atatccaatc gttgcacaag
    30481 gagattatca tgcttatgaa gcaaatcatc cggaagttta tggctttctt cgccaatttg
    30541 aaggacagca gctgcttgta ttaacaaatt tctatgataa agatacaagg atcgccatcc
    30601 cagaaggatt cacagaagcc aatattctta tcgataatta ttccgatact gttcttcatc
    30661 aaagtctgga gctaaaacca taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca
    30721 caaaacgtcg ttcctacgag ctattctcgt aagatcgacg ttttgtgttt tatttaaaat
    30781 aatcttcttc agaaaatgaa gagttttcat actctccttt atcgccataa aatttattgt
    30841 agcgttgttt gaatttacgg aaaataaaag taacgagtaa tttgactaac gtatagatag
    30901 gaataccaaa aataacgccc acaattccca ttagatcacc catgacaagt aaaacgatta
    30961 agatcgtaat cggatggatc tgtaaacgat cacccattac tcgaggaatc accaagtcac
    31021 cgtggagaag ttgtacggca aaccagacaa tgacaaattt gacagccata aaagtagagt
    31081 cttggaatgc aatgatcaaa cctggaaaga aggcgataaa cggcccaata tatggaatga
    31141 tacataacaa cccagcagaa atagctaaaa cactagcaaa ttctaatccg atcagtaaga
    31201 aacaaaccca gtagactgct ccaaggataa atgaagcaat gatttgcccc tttagaaatg
    31261 aacccaattg ttgattagca atctttgtca aatttttaaa atctgtacga aatgctggtg
    31321 ggacaatcgc taaaactgac tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga
    31381 caattggacc agtaaataaa gtaataaaaa tagctgtaac agttgaaaaa atgttgctga
    31441 tccgttgtgc gccactttcc caataatttc caataaatcc agctatatca gacgtcacgt
    31501 cgtcaataga agcaaaaaat tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt
    31561 gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa actactgatc aatgtctttg
    31621 cctgttcttc aagaaaagga ataagccata tgcttccaag ccccaataaa aataaaataa
    31681 tcacataaac gataatgact gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga
    31741 ctggttccaa aagataatac aagatcagtg caaacaataa tgggggcaag atagtagcga
    31801 taatgattcc gacaggcttg aatataaacg agatctgatg gaaaaaataa atgatcagcg
    31861 caagtaagat aactattcct aaaacatagt aagaggtttt tcctcctaaa aagcggatca
    31921 aacgattcgt tgtatctgtt tcttttttca ctctttttcc tcctttatct ataacttcat
    31981 tatagacaaa agcgggtata aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa
    32041 tcattcatat aaactttctc atcaattaaa ataagaaaac gcttgacagt tcttttaacc
    32101 atatgataaa ttaaatcctg ttgctaaaac gtaactatta cggtttgcga aaagcgaatc
    32161 ggaacattca aatgaaagaa gatgagaaga ggagtgtcat aaatgaaacg attgataggt
    32221 atattggcca tgctggtttt agcaggtatg ttaactgctt gcggggcaag tgggaaagcc
    32281 gaggacagta aagaaaaatt gagcgtgatg acgacctttt atcccatgta tgattttaca
    32341 aaagcaatcg tcggggatga aggagaagta gagttattga ttccagcagg gactgattcc
    32401 cacgattatg aaccttctgc taaagacatg gcaaagatcc aagatacaga tatctttgtt
    32461 tataatgatg aaaacatgga aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc
    32521 aatgtccata cgataaaagc gacagaaggt atgcttttac ttccgggaag tgaagaagga
    32581 catgatcatg accatgaaca cggggaagaa ggccatacac atgagctaga cccacatgta
    32641 tggctagctc caagtttagc aatcaagcag gtagcaaaca tacgtgatca gctgattgaa
    32701 gcttacccag aaaaacagga agtatggaca aaaaatgcag cagcctatac cgagaaactt
    32761 caagcactcc atcagcttta tcaagaaacc tttaagcaag caaaacaaag atcttttgtc
    32821 acgcaacatg ctgcattcaa ctatctagcg ttagaatatg gattgaatca agtatcgatt
    32881 gctggtcttt cttctagtga agaaccgtct gcggcgagaa ttgctgaact gaaacacttt
    32941 gttaaagaac atggaatcaa ctatatttat tttgaagaaa acgccaaaga tagtattgcc
    33001 agaacgttag caaacgaagc aggtgtatct ttagaagtat tgaatccttt ggaaggattg
    33061 acgaatgaac aaatagaaaa tggagaaaac tatctttcta tcatggaagc aaacctcgaa
    33121 gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag atactttgac ccccaaaaaa
    33181 gaaaagagcg tctacaacgg ttattttgaa gacgaggatg tacaagatcg ctctctttca
    33241 gattgggcag gaacttggca aacagtagat acgtatgtag aagatgggac ttttgatcct
    33301 gtttttgaat ataaagaaaa attgaatcag gataagacag cacaagaata taaagaatat
    33361 tatacgaagg gctatcaaac agatattgct tcaatcaaga tcagtgagaa agagatggca
    33421 tttacgttta aggatggaac aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc
    33481 ttgacctacg aggcaggaaa ccgtggcgta aggtatctat tcgaagcaga agataaggaa
    33541 agtggcgctt ttagatatat tcaattcagt gatcacacga ttgcaccggc tgactccgaa
    33601 cattttcatt tatatgcagg aaatgaaagt caagaagtat tgctgaaaga aatggagaat
    33661 tggccaacat tttatccaga tacaatggat ggacgtgaaa ttgcacagga aatgcttttg
    33721 cactgatagg caaaaaa
//
LOCUS       DAYYDS010000024        32209 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000024 DAYYDS010000000
VERSION     DAYYDS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.024"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..527)
                     /gene="yajC"
                     /locus_tag="ACHA02_001096"
     CDS             complement(147..527)
                     /gene="yajC"
                     /locus_tag="ACHA02_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8669460.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(624..1769)
                     /gene="tgt"
                     /locus_tag="ACHA02_001097"
     CDS             complement(624..1769)
                     /gene="tgt"
                     /locus_tag="ACHA02_001097"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8669461.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1987..2685
                     /locus_tag="ACHA02_001098"
     CDS             1987..2685
                     /locus_tag="ACHA02_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8669462.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2853..5492)
                     /locus_tag="ACHA02_001099"
     CDS             complement(2853..5492)
                     /locus_tag="ACHA02_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8669463.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6111..7142)
                     /gene="queA"
                     /locus_tag="ACHA02_001100"
     CDS             complement(6111..7142)
                     /gene="queA"
                     /locus_tag="ACHA02_001100"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8669464.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7335..8510
                     /locus_tag="ACHA02_001101"
     CDS             7335..8510
                     /locus_tag="ACHA02_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8669465.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8514..9155
                     /locus_tag="ACHA02_001102"
     CDS             8514..9155
                     /locus_tag="ACHA02_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8669466.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9152..10069
                     /locus_tag="ACHA02_001103"
     CDS             9152..10069
                     /locus_tag="ACHA02_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8669467.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10066..10737
                     /locus_tag="ACHA02_001104"
     CDS             10066..10737
                     /locus_tag="ACHA02_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8669468.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11278..11766)
                     /locus_tag="ACHA02_001105"
     CDS             complement(11278..11766)
                     /locus_tag="ACHA02_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8669469.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11967..12233)
                     /locus_tag="ACHA02_001106"
     CDS             complement(11967..12233)
                     /locus_tag="ACHA02_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8669470.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12233..12496)
                     /locus_tag="ACHA02_001107"
     CDS             complement(12233..12496)
                     /locus_tag="ACHA02_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8669471.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            13017..13157
                     /locus_tag="ACHA02_001108"
                     /pseudo
     CDS             13017..13157
                     /locus_tag="ACHA02_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13299..15122)
                     /locus_tag="ACHA02_001109"
     CDS             complement(13299..15122)
                     /locus_tag="ACHA02_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8669472.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15720..18425)
                     /locus_tag="ACHA02_001110"
                     /pseudo
     CDS             complement(15720..18425)
                     /locus_tag="ACHA02_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
     gene            complement(18731..19678)
                     /locus_tag="ACHA02_001111"
     CDS             complement(18731..19678)
                     /locus_tag="ACHA02_001111"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8669473.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19868..20137
                     /gene="rpsN"
                     /locus_tag="ACHA02_001112"
     CDS             19868..20137
                     /gene="rpsN"
                     /locus_tag="ACHA02_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8669474.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20313..20675)
                     /locus_tag="ACHA02_001113"
     CDS             complement(20313..20675)
                     /locus_tag="ACHA02_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8669475.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20686..21807)
                     /gene="alr"
                     /locus_tag="ACHA02_001114"
     CDS             complement(20686..21807)
                     /gene="alr"
                     /locus_tag="ACHA02_001114"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8669476.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21823..22173)
                     /gene="acpS"
                     /locus_tag="ACHA02_001115"
     CDS             complement(21823..22173)
                     /gene="acpS"
                     /locus_tag="ACHA02_001115"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8669477.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22303..23814)
                     /locus_tag="ACHA02_001116"
     CDS             complement(22303..23814)
                     /locus_tag="ACHA02_001116"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8669478.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24136..25488)
                     /gene="murF"
                     /locus_tag="ACHA02_001117"
     CDS             complement(24136..25488)
                     /gene="murF"
                     /locus_tag="ACHA02_001117"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8669479.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25641..26717)
                     /locus_tag="ACHA02_001118"
     CDS             complement(25641..26717)
                     /locus_tag="ACHA02_001118"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8669480.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26913..28658
                     /locus_tag="ACHA02_001119"
     CDS             26913..28658
                     /locus_tag="ACHA02_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8669481.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28938..29375)
                     /locus_tag="ACHA02_001120"
     CDS             complement(28938..29375)
                     /locus_tag="ACHA02_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8669482.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29359..30732)
                     /locus_tag="ACHA02_001121"
     CDS             complement(29359..30732)
                     /locus_tag="ACHA02_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669483.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31054..31542)
                     /locus_tag="ACHA02_001122"
     CDS             complement(31054..31542)
                     /locus_tag="ACHA02_001122"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8669484.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31564..32166)
                     /locus_tag="ACHA02_001123"
     CDS             complement(31564..32166)
                     /locus_tag="ACHA02_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8669485.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9210 a         6883 c         5942 g        10174 t
ORIGIN      
        1 taaagttatt tattattatt tatatgacac acccgattat tcaatattga gcaatatatc
       61 ccatgctctt atctctttct tttttgaata aaaaatgagg taggacatct ttctgcccta
      121 cctcgtcttt aaataagctt tgatacttat ttttcttcct tctcttctgt ctcttccagc
      181 gtttctgttg tttcagtttt tacttcaact gtctcagctg gttcttctgt tgtttcaaca
      241 gtagtcgttg catcatttgt tacgacagtc cctggtttga ccgttttgat tgctgcacga
      301 tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg tccgtttgtc attgtcgatt
      361 tctgaaacaa ctccatgtaa tccgccgatc gttacgactg aatcacctgg tttcatgcta
      421 tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg aacgtgacat aaaaaaccac
      481 attgctaata gcgcgataaa catcacaagc attggtaatg ttcccatttt tttcacctca
      541 ctaattctgt atctacaaaa aacactttag cagaaaaatg ctgataacac tagtttttta
      601 cttaattttt aaaagatttt cctttagaag ctttttgcgt tttctttatt gaacccgtat
      661 tcttcaaaaa aggcttctct aaattcaagc aaattatcat ccatgattgc ttgacgtact
      721 tgtttcatta gattcaacaa gaagtataaa ttgtggtaag aagtcaaacg aatcccaaat
      781 gtttcatcac atttgatcaa atggcggata taggcacgtg tatagttttt acatgtataa
      841 caatcacatt tctcatcaat tggacggaaa tcatgggcat atttcgcatt tttcactact
      901 agacggcctt gagaagtcat acatgtgcca tttcgagcga ttcgagtagg caagacacag
      961 tcaaacatgt ctactccacg gataacacca tcgatcaatg aatcagcagc accgacaccc
     1021 atcaagtaac gtggtttgtt ttcggggatc aacggtgtag tgaattctaa tacacgattc
     1081 atttctgatt taggttcccc gacagataac ccgccgattg agtaaccagg gaaatccatc
     1141 ccgactagat ctttcgcgct ttgacgacga agatcttcaa accctgctcc ttggatgatt
     1201 ccaaataatc cttgacggtc tggattagca tgagctttca atcctcgttc tgcccatcga
     1261 gacgtacgtt ctactgattt tttcacgtag tcgtaacttt catcgaatgg tggacattcg
     1321 tcaaaactca tcatgatatc agagcccaat ttattttgga tgttgattgc tttttctggt
     1381 gataaaaaca tacgcgaacc gttcaagtga tttttgaaat gcacgccttc ttcttcgata
     1441 ttccgcatat cacttaatga aaaaacttga aagccgcctg aatctgttaa aatcggttga
     1501 tcccagttca taaatttatg cagtccgcca gcttcttcta ctaagtcttc accagggcgt
     1561 agccataaat gatatgtatt acttaaaata acgccagcac ccatttcttt caactcttct
     1621 ggggacattg tttttactgt agctaatgtt cctactggca tgaacattgg tgtggggaaa
     1681 gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag tatgtttttc tttcttgatt
     1741 aaacgatagc gaatggcagg ttgcgtcatt catttcccta ctttctatat aaaatatctt
     1801 gacaactccc accatcataa tcgtttcaag tgctttttgc aagttccctc ggcttccttc
     1861 tactttattt aatattcttt tttttatcct ttttttatga ataacaaaaa aaagaaaaac
     1921 attatgacag aaatattttt tgatacatgg tatattggtg ttattaaaga aaaggaggga
     1981 aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa aagagagtct aaaaggacgt
     2041 tggggacaag ctgttttact aaatctaatc cctacattga ttggtattgc tactattctt
     2101 cttattatga ttcctattat cttgtttgcc accaccatat atgatggatc agcgagcgtc
     2161 tcagaagcga caacttcagg aggcggtagt tcaggaggcg gtggcggaat tatttccagc
     2221 atcattagcg ctttgttcat gagcggtatt tcttggactt atcttgatat tttacgtgga
     2281 caaaaagatc agatcgaacc attcaaagat gcatttcgtg gattcaaagg tttgtttatc
     2341 ggcggagtga tcagcttagc tgtgctgatc tcgatcttta caacattatg gggtttgcta
     2401 ttagttattc ctggaatcat taaaagctat tcttattctc aaagctattt tatttactac
     2461 gatatcgtcc aagaaacagg acaaacacct aaagtactgg atacgatcac tgcaagtcga
     2521 aaattgatgg atggttataa aggacaactt ttctggttag atctttcatt tatcggctgg
     2581 catatcttag cattagcaac tgctggtatt ggttatctat ggctcagccc atacatttct
     2641 gcaacaaaag cagcatttta tgaacaattg ccaaaagata tttaaaagag agtgtgactt
     2701 gtgaccgagg gggtggcagg agaacaccgt ttatcgagag gcagtaaaag aaacattcag
     2761 cccattagca aaaaggaggt gtgacaacat tcatgtcaca cctccttttt gtatagttcc
     2821 agtaaaaaga aaatcaactt agaaacagtt aatcattcct cttcttctgc ttccggtaat
     2881 cgatggaact tgatcagttc attcaccgcg attggtacga tagataaaat ccctaccaag
     2941 aaccagtgat tcaaactgat gtgtactagg ccgaatagtt cttgcgtaaa tggcagcaaa
     3001 gcaacagccg ttgtaatagc taatgccagt aacgccattt ctaccaaagc tttattggtc
     3061 gctaaattga ctttgaagat cgattggctg ctgcgtacat tgaacacatg cagaatcgat
     3121 gaataagcta agatcaagaa tgcaacagtt tgaccgacat gctgacttgc gtctacccat
     3181 gggctgatag tatcgacata actacctaaa taataaccaa acaaggttac gatggtgaag
     3241 acccctgcat ttatggcaat tttttgcaat aaacctcgag caaagatccc ttcatttttc
     3301 ggaatgggtg gctgatccat gatgccgtgt tccgctggct ctcttcctaa agcaaatcct
     3361 ggaagtccat cagcaactac attgacaaac agtagttgca cagctgtaaa tggtgctccc
     3421 catcctaaca gcatcgcaat caaaacaata aagatctccg aaatattaca gcttagcaaa
     3481 aagttgatag ctttacgaat attctgataa accgaacgtc cttgactgac agcgtctacg
     3541 atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg cactttgcgc cacatctgtt
     3601 cctgtgatcc ccatggcaca cccaacgtta cttgctttca acgctggtgc atcattcaca
     3661 ccatccccag tcatcgctac gactgcaccc gtacgttgcc aagatttgac gatccggatc
     3721 ttgtcttctg gcgttacccg agcatagaca gagagagctt tgacacgcgc atctaattct
     3781 tcgtctgata attgatgcag ttctgatcct gaaagtgctt ctgacggatc attcaagata
     3841 cctaattctt tcgctatggc actggcagtc accacatgat ctccagtgat catgactgtt
     3901 ttgatgccag cctttttcgc tcgcttgatt gctcctttac tttctggacg tggtggatcg
     3961 atcattccga tcagaccaat caaacgtaag tctttttcca gtgcttcaga agaaatatct
     4021 tgcggttctt cgtcataagt agcataacca acagcaatca ctctcagtgc ccgtttacca
     4081 aagcgatcat taacgatcgc cgcttgttct acatcgcctg aactaaagcg cggagcaaga
     4141 acatcgaatg ctcctttggt gattgagatg tatttttcgc ccatttggtg aactgttgtc
     4201 atcatttttc gttcagaatc aaaaggaagc tcgtttactc gtggatattt ttcttctaat
     4261 tctgcctttg tatgataatt ctcttcaaca gcccttacga tggctacttc tgttggatta
     4321 cctttgataa caaggtcgtc atcttcttgc tcgaccgtca catctgtaca tagtgcagcc
     4381 attttcaata tttccatcgc gctgttcgtc attgaatctt cgatattcgt tacttcatct
     4441 tctttgctcc atacccggcg gacgcgcatt tcattttgtg tcagcgttcc tgttttgtct
     4501 gagcagataa cattagcggt tcctagcgtt tcaactgctg gcaactgtcg gatgatcgca
     4561 tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca acgtaacaat aacagtcaag
     4621 gtttccggta cagctgcaac agccaaggaa atcgaagtca tgaacatctc aaggagtggt
     4681 tctccttgca gttcaccaat cacaaatact aaagccgcag ccgctaaagc gatcatactg
     4741 attcgtttgc ctagctgatt caatcggacc tgtaaaggtg ttttttgcat agtattttcg
     4801 ttcaaaagat ccgcgatctt acccatttct gtagccatgc caatggcagt gacgatcgct
     4861 ttcccacgac ctgcagcaac cgtacatcct ttaaatacca tattaagttg gtcacctaat
     4921 gactcatctt cttcagcaat aaatgctgca tctttttcaa ctgcttcact ttctccagtc
     4981 aacgccgatt cttctacacg taattgtgat gcttcgatca aacgggcatc ggcagaaata
     5041 gcatcaccgg actctaaaac aagaatatca cccactacca gttgctctga ctcaacagtg
     5101 gtcactttgc cgtctcgtat gacagttgcc gtttgtttat tcatatcttg caaggattcc
     5161 agtgcttttt ctgcgtttcc ttcttggaca atcgccaata ccgagttgat cacaacgata
     5221 gcgatgatca gcagtccttc aaataaatcc ccatgttctg tagcaaatgc ggtataaaag
     5281 gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat cggacaagct tcgaaagaat
     5341 tttttgatca tcgattcttt cggcgcctca tcgaatttat tcaatccctg cttttttcgc
     5401 cgttcatcca cttcttgtga agacaaccca gtatctggat ctgcttctgt taccttcttt
     5461 acttcttcta ttgtctgctt gtattcttcc atcctattcc tcctttaaaa tcttcgtaca
     5521 aaaaaaggta cttattttac tataacatcc tttttttatt tacttcaaat aaagggctta
     5581 tcgattattt gttaagaaag cgtaaaattt aaagtgctta tatttgcaaa cgctaccaaa
     5641 ggatgctatt atatagatgt aaggaaggag gataccatca aatagacaga agaatttctc
     5701 gatagaacag tgtaagcaaa caaagttgtt agaagtacag gtttcaccca gacatgcgtg
     5761 tagagtacaa acctataaat agtgaatgcg tatggatgtt tggatcacga agtatccgaa
     5821 acaagaagta atgcgatacg taacaattga ataacgtaaa cgtctgaaaa ttgaatagga
     5881 acaagagatg tttcaaatgt tgaagatgta acgaaaacaa aatttttgtt taacatgaag
     5941 aaattcaaca ccgtgctttg catgtatgat ggaaagttac aatcatttct gtttatttga
     6001 atccttccga ggtacaagaa agccgagctt atcgagagct cggctttttc tttgtcataa
     6061 aatctttgta aagctttttt gttattcaaa actagcggct tttcataacc ttatttcacg
     6121 aacatcgcat ctccaaaact aaagaaacga tagcgctctt caatcgcatg ctggtaagca
     6181 gataaagtca attctcttcc tgcaaaggca ctaactaaca tgactaaagt cgattttggc
     6241 aaatggaaat tagttgagaa agcttctacc actttgaatt catagccagg cgtaatgaaa
     6301 atatcagtcc atccgctgtc tgctttgatt tctccattaa atttcgttcc aatcgtttct
     6361 aatgttcgaa ttgaagttgt tccgacagcc acgattttcc cgccattttt acgtacttgg
     6421 ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt cactatgcat atggtgttct
     6481 tcaattttat ctacgctgac aggtcggaat gtacctaacc ctacatgtag tgtcagatag
     6541 actaattcaa caccctttgc ttcgatctca tcaagtaatt ctttcgtaaa atgcaatcca
     6601 gctgttggcg ctgcagcaga accattttct ttcgcataga cagtttgata gcgatctggg
     6661 tcttccagac gttctttgat atatggaggc aatggcattt ctcccaatga ttcaagaatc
     6721 tctagaaaaa ttccttcata ctcaaagtca atgatccgtc cgccgtgttc caattcttct
     6781 ttcactactg cttgcaatct gccatcacca aaagagattc ttgtcccaac tttggcacgt
     6841 ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg tattcgttag aagaaggact
     6901 tctaaatgcg caccagtatc tggtttttct ccatataaac gagcaggcag aactcgtgta
     6961 ttattcataa ctagcgcatc accaggctgt aactcttcaa tgatttcatg gaaatgttta
     7021 tcttccatct ctccagtttt ccgatccaaa acaagtaaac gagaatgatc gcggtctttt
     7081 aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat caaaatcttc tgttgttaac
     7141 atatcgtcac tcactttcta tcatgtgctt catcattcta tcatgttttt cgttctgttg
     7201 gcaaaatcag cactttttca cttctatttt ttgttttcct aaataaaaaa actaccctat
     7261 ttgtattttt tcattgtttt aggcatacta gtaatagata gttcttcatt gaaaatagaa
     7321 aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa aatttataaa ggtggaaaag
     7381 tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga gttcatttgt tttatcggta
     7441 cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa ccgaatgact gatccaacac
     7501 aagggaaaat tttgattgat ggaaaatcca tccaagaaat tgatccagtg gaattgcgac
     7561 gtcagatcgg ctatgttatc caaagtattg gattgatgcc tcacatgacg atccgtgaaa
     7621 atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga agaacggaat aaaacagctg
     7681 aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt agatcgctac ccacatgaat
     7741 tatctggtgg tcagcagcag cgtattggag tcgttcgtgc ccttgcagcg aatcaagata
     7801 tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat cactcgtgat tccttgcaag
     7861 accttgtcaa agatttacaa gagcgtttgg gaaaaacgat cgtatttgta acccatgaca
     7921 tggatgaagc gattaaacta gcaagccgta tcgccatcat gagtgaaggc cgtgtgatcc
     7981 aatttgatac accagaaaat attttacgtc atcctgcaaa cgatttcgta gaagagttga
     8041 ttggagaaga tcgcctacta caagccaagc caaatgcgac aagagtcggc gaagttatgc
     8101 taaatacagc aatcacgatt acaccggaaa aatcattaca agaagcaatc aaactgatga
     8161 gggaaaaacg tgtagatacg ttgttagtta ccgataattc gaatgtatta aaaggattta
     8221 tcgatgtaga aacgttgaat aaaaaacgag gaaaagtatc cagtgtcggc gacattttga
     8281 ataaacatgt tttttacgta aaagaatccg cctatcttcg tgataccttg caacgcattt
     8341 taaaacgcgg gctgaaatac gtgccggtcg ttgatgaaca gaacaaagtc gtaggtatcc
     8401 tgactcgtgc atcattagtg gatatcgtct acgatgtgat ttggggagaa gatcccgcaa
     8461 ctactgatgt tgcagagtca gctactcctg aagaatctaa ggaggtctga cctatgcagc
     8521 agttttttca tcagcacggt tcagaaattc tctcaaaagg ttttgaacat ctttatattt
     8581 caggacttgc tttgatcatt ggtatcatca ttgctgtacc gcttggtgtc ttactgacac
     8641 gttttccaaa agctgccacg attgtgattg gcttaaccag tgccttgcag acaatccctt
     8701 ctcttgcttt attggcattg atgatcccgc tatttggcgt aggtaaatta cctgcgatta
     8761 tcgcactctt tatttattca ttattgccga ttttacgtaa tacgtacatt gggatgaaaa
     8821 atgtcgattg gaattatcgg gatgtggcaa aaggcatggg gatgactgag ttccaatcca
     8881 ttttttccgt tgaattgccc attgctatgc caacgatcat ggctggtatt cgtttagctg
     8941 ccgtttacgt gattgcatgg gctacacttg cctcttatat cggcgctggt ggtttaggcg
     9001 atttgatttt tagtggactg aataattacc aaccagatct cattttggca ggtacgatcc
     9061 ctgtaacaat tttagcacta ttggctgatt ggcttctagg tctcttggaa caccggctga
     9121 caccgatcgc acttagagag gagaatgaag aatgaaacgt atactaaaaa tgaccatggt
     9181 tcttttctct cttcttatac ttgcgagttg ttccttccct ggtcttgcca gcaatacaga
     9241 tgaagacacc atttcgatca caggtggaat cacttcagaa gcacagatct tagcaagtct
     9301 agttgccgga atggttgaac attatacaga taaaaatacc gcaatcatca acaatctggc
     9361 tactacaact atcaaccatc aagcgatgat gaacggagat gcttcaattt cagcggctcg
     9421 ctacacagga acagatttaa cgactacatt gaaccttcct cctgaaaaag atccgaaaaa
     9481 agcgtttgct accgtgaaag atgaatttga gaaaagatac ggtcaaacgt ggttcccgtc
     9541 ctatagtttt gaaaatacgt atgtctttct tgttcgaaaa gacaccgcac aaaaatacca
     9601 tctatctaaa gtcagcgatc tgaaaaatgt agcggatgaa ttagtagctg gtgtagatac
     9661 ttcttggatc aatcgtaaag gagacggtta tgacggcttc caagaaactt atggcttctc
     9721 cttcaattcg atattaccta tgcagattgg acttgtttat gacgcagtcg aagcaggaaa
     9781 aatggatatt gttcttggct actctacgga tggacggatc gccagttatg atctagtcat
     9841 gttggaagat gatcgtcaat tcttcccacc atatgatgct gctccagttg tagacaacaa
     9901 gatattagct gatacgcctc atttaaaaga agccttaaat aagttaggtc ataccatcag
     9961 tacggaaaaa atgcaggaat tgaactacga agcagataat aatctagtgg aaccatcagt
    10021 agtagcacaa cgtttcttac aagaaaacca ctattttgaa gggaagtgaa gcaattggat
    10081 aaaatgaatg tctttcaaca atttttctat tatttccagg aaaatggctc ctatatcttt
    10141 gcacaattta tccgtcactt cctgatttct atctatggtg tcctctttgc agcagttgtc
    10201 gggattccgg ttgggatcat gatttctagg agacgtaaat tagcaaactg ggtgattcgt
    10261 ttggcgaata tcatccagac gatcccttct ctagccatga tctccatttt aatcattgga
    10321 ttaggcctgg gagtcaatgt cgtgattgtt actgtatttt tgtattccct cttaccgatc
    10381 atcaagaata cgtataccgg gatgatccaa gtagacaaaa acatccttga tgtcggcaaa
    10441 ggcatgggga tgactgctag acaacgacta ttcatggtag agttaccact ctctgtttct
    10501 gttatcatgg caggcataag aaacgcgtta gttgttgcca tcgggataac cgcgatcggt
    10561 gcattcgtcg gtgctggtgg tcttggagat atcattatcc gaggaaccaa tgcaacagag
    10621 ggcacatcga ttattttagc aggtgcattg ccaactgcat taatggcgat catcacggac
    10681 tggcttttag gtattttgga aagacggcta gatccagcca gccgtacatc acgctgatgt
    10741 tattgtaaaa aacaaaagct tctgcgacca tttgttggtc gcaaaggctt ttttagttct
    10801 gtcattttta aggcaataat ccactctgat tcttccctcc ttatgggtaa agagttgctt
    10861 ttcttagcta tttatccatt ccgtatactc caacttcata ctgccaacgg gttcatatct
    10921 gcatccaccg actaagtttt tttcttatcc gctaaggcat tttgccaatc cattttccca
    10981 tcactcgtca tggaattcat accacatgat ctctaaatac tcgtctgctt gttctttgaa
    11041 ttgggcatca attcttacat cctttggcgt ggctttcatt taagcctttt ccatcagtcc
    11101 tcgattgttc gatgccttca gctgcatcat caaaactggt gactgccgta tcggtaactt
    11161 cgatcatctt ttatacaatc gggaacttgc catcctcatc cttggctaaa aacatatcta
    11221 gccattccag cttctcgcca tgggaggttt gcggttccta aaaccggtac acgattctta
    11281 tggactgcat ccgtcgcaac tagacttggc gatacgatga tccttcacca gaagagcacc
    11341 cccgtaaacc aattgatcga tatattgcca ataagaaaag acattggcgt aaaagatatc
    11401 gatccctcgt gatgcattgc cgttgatact gctattcatg gccgaaagcg ccacgacttt
    11461 aacttcttcc gtttgagtga cattcatgtt cggcaatgtc gctttggata cccgctctgc
    11521 taaaggaacg acactggtat tgtatttcgt atctgtatct tttccaaaag accatgccaa
    11581 cgaatcctct gggaaccaat aagacgactc cggttgattg tccattgctt ttttcaacat
    11641 acttttggct gtttctcgat aaacgacatt tccaattgtc ttcgcctcac tttggcgata
    11701 atcgatggtc gttgccactg ccaacactaa caacgatgct ccaattttac tgattttcct
    11761 tgtcacactt cttcctcctt gttttacata aacccgtcaa cattccattc ctaaactcaa
    11821 acaatgaccc ttcaaaggtg gagatatagg aacaaattaa agaaatcatg caataaatcc
    11881 aagaaggttg gttttagaac aaaaaaagag tctccttttt ggggagactc tttgttaact
    11941 atctaaaaag gttggctctt ctcacttcac tcataatgtg tccaggcaga ataaatttct
    12001 acaactttat tttcctcatc aactttgtaa accactcgat gctgtctatt cagcctgcgt
    12061 gagtatctgc cttcatgtgt aggtctcagc ttttcaaaag actgcgtggg catatatgga
    12121 tcttccttca atgtctgaat cacttcttca aattgtttct ttaaattcgt ttgcttgatt
    12181 tttcttaaat ccactttcgc agagtttttg attgcgactg tatagttact catcaaagag
    12241 cttcccaatc aatatcatcg atattcgtaa agctactgtc gtctttttcg cggtctcgta
    12301 ctttatctaa agtacctgtt tgctcaagaa aaagggtttc ttgaatcgct ctccagtctt
    12361 ccagcccaat caatacagca ttgtttccac tacgatcact aataatctcg atttcagtat
    12421 gattttcatt tacctctttc aataactgat aaaaattttt acgagcagtg ctaggattca
    12481 tcgttgtatt agccattgtc tctcctcctt tttctgatat aattatagcg tacttgatta
    12541 agtacgtcaa aaaaatacaa taaagcactt aaattcagca aacttttcct cttttttaca
    12601 tagagaaaaa gacccctttt tagggaggat ctttgtccac gctcacttct tactccttta
    12661 cgactagcgg tagccaaacg taaagtaatt ggtaacaacg tatcatcttt agcaaaggtc
    12721 gaatccaaag cctgtgagtc tatgcaacaa caccaataca cgaaggttcc tcgttgcagt
    12781 gtgacttgat tgatatcttg aataacctga ggatacgcac gaaagatttg taacggttat
    12841 tcaaaactga ttttggccgt caagtcttct actgcctcaa aaatgattgc ctctttcgtc
    12901 tcctgtcatt catgttcact atcgatttga tacttgctta gaccgttggg tttgtgaatc
    12961 accaattaaa aatggcactg agttatggta acaaaaaatg gtcacttgac gaggctgaga
    13021 ttcctactta tgaagacaca tggcacttaa gagtcgaggt aaaggaagaa gctgaaaata
    13081 taggaggctg tacattattt ggcaaaggat tcggaaatta tgatcaagac atcaaaatca
    13141 aaatgtttta cagctaaata ggaagaataa taacatttca tacgctgttc ccatctttaa
    13201 tggcttatag agggcacctt tagctgagaa taagacaaaa cagaccctct agcttcaatt
    13261 aagagctaga gggtctgttt ttgattttaa aacttggttt atttcttcaa atggtagcta
    13321 taggtgctga caacgatatt ttcacgccaa gacaatcgca atcttaaccg caaacttgtt
    13381 tggttatgga gaatattctg ccagcgtctg tttggtacaa attgaggaat caacacagta
    13441 gttgtatagt tttgtttcgc tgcattttta cttacgatat ctacataacg gataattgga
    13501 ttttcaatcg agcgataaga agagtggacg attgaaaaac ggacatctgg gaaatgcttt
    13561 ttaaattctg cttcgatttc tttttctttt tcgacatctt cgtctagcga aacatgcatc
    13621 gcaacgacat aatcaccgat cgaacgtgcg taattcaacg caccgatatt tacccgtgtc
    13681 acattcccga ctaatacaag aacggtattc ccgtcataat catgtagttt tgcatcattt
    13741 tctaaacgaa gctgttctgc tactttttta taatgatcat ggatcttata aaaaatgaac
    13801 ataacgattg gcataatgat gaagaaaggc caaatgtccc ctaaccgata gacaaacaaa
    13861 atcgcaatga ttgcatatga gatgaacgca cccgtaatat tggcaatcga tttgcttaac
    13921 catctttttc cttctttttt ccatttgacg accatccctg tttgagataa agcaaatgga
    13981 atgaacacgc cgatagagta taacggaatc aagcgttctg ttgagccatg gaaaataaac
    14041 agcaatacga tcgaacctag cgctagtgtg ataatcccat tggaatagcc tagtcgatcc
    14101 ccacgatctt gatacatgtg aggcatgaat ttatcttttg ccagattgta tgcaagaact
    14161 ggaaaagcag aaaaaccggt gttagcagca actgctaaaa tcagcgctgt agcaaattga
    14221 aggacgtaat acaaaatacc atgtccaaag acagctttac caatttgaga caaaacagtg
    14281 acttcttttt caggaacgat cccataccaa tagttgataa atgtgatccc cacgaagaag
    14341 aaacctaaga tcaatgccat catcgtcaat gtagcagcgg cattttttgc tcttggcttc
    14401 ttaaagaatg gtacagcatt actaatcgcc tccaccccag ttaaagagga agaaccagaa
    14461 gaaaatgctc gtaaaatcaa agcgatagaa acacccggca cagccgctcc tggaagtgca
    14521 gtagcttgca atggcacagc acctgtcacg atattatata aaccaacaac gatcagtaac
    14581 gagatcacaa ggataaatgt gtacaccgga aataatagga acgatgcaga ttccctcagc
    14641 ccacgaagat tcagcatcat cagtagcaaa acgattgtca cagaaatagc gacttgatga
    14701 ccgtagagtg ctggcaccgc tgatgtgatc gcttcagcac ctgctgagac agatactgct
    14761 actgtcaaca tgtagtcgat caataaagag ccaccggaaa tcaagccagc attctttcca
    14821 agattctcac tgctgacgac atatgctccc ccgccatgag gataagcatg gatgatttga
    14881 cgatatgata gcgttaatga aatcaataaa ataataacaa aagcggcaat gggtagcgaa
    14941 taccagatgg ctgcagcaga caaagcgact aaaacgacaa cgatttgttc cgtaccatat
    15001 gcaatagatg ataacgcatc agacgacaat aatgccaaag cagcaaatcg ggttaatttg
    15061 tgctcatcat tttcagcaga ttttaaaggc ttacctacta acaacctttt taagtaatcc
    15121 aatttctaaa ctcctttaca cacaatttta cttcaagttt tatagtcgaa aatttatagt
    15181 aaacaagatg ttatagtacg ctcactgacc ttaaaagtca ataaacttta agaaaataat
    15241 tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc tttaatacag caaaaatcta
    15301 acattttttt atgggctttt tctttgaatt ctataataaa aggatagaac ttgacttaga
    15361 aaatcaagtt ctatcctttt tgatatctta acttttggaa gagagatgta aggctgcttt
    15421 ctacccatca ttcttactac tcgaagcata gcgcagttgt cacaacctct tgataaacgg
    15481 tgtttaaaag cttgcatctg cggtaataag cccaaccaaa ttaaaaacat aagaagctgt
    15541 ttttatttgg tcgttcttat tacttgatgc agagcagctt ttttacaacc tcttggtaca
    15601 cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa acggcaaaat atgaggaact
    15661 atttttccat ttttttctct aatactcagg tcaaaacact gatttcacaa cctctttttt
    15721 taatgaaaaa tatggctctc atgcgccgct tctttgaatt tcgtcatttt ttcttcgatg
    15781 aacggataga ttgccgttcc tacgattcca aagacaacaa acaaaccgat catgatccag
    15841 attgctttcg tagcatttgg ccaataaatt cctcctgcag attcccgtaa caagtttacc
    15901 gcatgagtaa atggcaagaa cggattgatc atttggaaga atttccctga gacttggatc
    15961 ggatagttcc cgcctccgcc agagatcgac agtactaaga tgataatagc gatcccttta
    16021 ccgacattat cgaataatgc agccaagaca tagacgatca tcatgaacgc taaggcgatc
    16081 aagacagcaa atgacacgct gtagaccgga ttttggacat ctactcctag taagaaaata
    16141 ttccctaatg taacgatcaa gctttgcgca atcgccattg tcaaaaatgt cagcatacga
    16201 gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt tctttcctaa atagtattct
    16261 gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca gacatagtgc agtataaaac
    16321 ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg cattggtttg tagctcaaca
    16381 ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt tcaataattt gatgacttcg
    16441 cctagatcga cttcttgttc gccttttcgg atcgcgtctg ctgctttttg gataccagct
    16501 ttgatatttg gccagtcatt gttgatcaag tccgcagcag cgttcacagc tgtttcgact
    16561 tctggcattt tttcatttac tgttgtcaat gtaccggtga tttcattttt tagtgctggc
    16621 caatcatttt ggataaaatc agcggccaat cctaattttt gctgaatgat cggtaattca
    16681 ttgttgtata ggtctgcccc ttcattgatg ccattgacga tggtatccat atttccattc
    16741 agcattgtat tggcatcatg gatttcttgt ccaattgcag gcatttcttt ttgatatttt
    16801 tctaaaattg ccactgcatt cgttacagtt gctttcgttg agctgagaag cgaatcaaag
    16861 tcgatctagt ccgctttgct cagtgcgcct tgtgcagtcc caatcgtatc gatgacttgg
    16921 tctagtagag aagtgacagt ggaactgatt tgacctgtat tgataccagc aatgatgcca
    16981 ttgatattat taacaacggc atcaatttga tctagcaatt catcaatagc cttcgtgtct
    17041 tcattttgaa cagctgtgtt caaagcagat aggcggtttt gcagattgct tagcaagggt
    17101 tttaattgat ttagctgatc aataattggc tgtaatttcg tatttccagc acttgtttgc
    17161 aatttagtca ggaactcgac cagtttatcg attgcatttt gttgttgaga tattccgtca
    17221 ataaagccgt tcaatacatt gctgatgttt ttcttttcat catcagtcag cttaccatct
    17281 tcaatagctg ttcttacagt atctattaac gaacctgtcg ttgttgccac ttgtccaatt
    17341 gacttcaatg taacatctac actgtttgtg atgcttggta tcgcatcttt cagctgagag
    17401 gcacctgatt ttgtttggtt tgctagatca cttgcctgat tttctaaatt tttcacatct
    17461 ggcaagatat cttgtacttg ttggatgatc gtcaagcctt gttttgcttc atcgattcct
    17521 tggttcattg tctcttggat cgaagcaaaa tcgccgtcga tctctgctag ttgcttaccg
    17581 gcattttgga tctctgggat tttttcttgc aaatcaagga tgatttttgc ttgatctttc
    17641 aactcaggca ttttgtcatt caatgcaacg actttttcac ccatctcatc tactttaggg
    17701 atgtaatcca cgaattcatt tgctttattc agtttttcct tgatttcagg tagttgactt
    17761 tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt ccaaattttc atctgttgat
    17821 aaaatcatat ctttaacttt attgatactt actaggttcg tatcgatgtc gtaacctatc
    17881 tcattgaata ctttcaataa agtcgaactg gctgttttga taaattcatt tgtgatttgc
    17941 gattggatcg aacttgctcc tttatcggtg attttcggtg caatcgcatt gattttttca
    18001 tttaccgtat attcgatttt tggtttcttg atatcaccac tagtaaaact cagcagatct
    18061 tctgagaaat cctttggcaa gtaaatacct gcgtaatact ttccggaacg gactccgtct
    18121 tctaattgtt cttttgaatc aacgaattgc catcctaatt gtttattttt atgaagcgat
    18181 tcgatgactt gctccccaat ggccacttct ttaccttgaa actctgctgg cttgtctgca
    18241 ctgtaaacag caattggcag ctctccagta tttccatatg gatcccataa tgctttgatg
    18301 ttaaaccacg catatagcga agggatgatc atgatcgcga tgatcagaag tgttgcaatc
    18361 gggtttttga aaatccgttt ccagtcaagc aaatataatc gaagcgtatt tttgattgac
    18421 tgcatagttc tcccccttct tcttttttca gctgaattac tttaccatgt atttcatact
    18481 tttttcaatc ggcttttttc aaaaaagtcg aatttttgga catatcacgg caattgtctc
    18541 attgtaatct cttacatatt gagaaataat taaggattac agcctttact ttatcatgaa
    18601 ataaaaggga aagataattt tatgccaaag tttttatcta ttatacgtat acgcaaaaaa
    18661 gctaaaacat tttttgtctt tgcccctaca aaaatgtttc agcttttttc gttttttatt
    18721 cctgtatttc ttatttttcg aatgacagga tgattttccc agcaggagga tgtccttcaa
    18781 ctagctgatg tgcttggatg acatgcttaa gtgaagcaga cagattcatt gaaataaaaa
    18841 catgaaaggc tcctttttgg taggctttga ttatgccagc aaaggcttct gcatctagat
    18901 attcctttct tggaacaaac gattcataaa agccttcctt tttttgccgc aaatctagat
    18961 ctgggaggtc atttaatgca acatacctgc cgcctggttt catgatctgg atgccggttt
    19021 ctcctttgat acttcctttc gtcgcatcga ttacaaggtc tgcctgatca gcaaattgca
    19081 gacctggatt tgttttatca taagcagcaa atgctgaagc tcctaatttt tttacctttt
    19141 cctcattttt actggatgca ctggttaaga ttcggattcc cttttcatgg agaagctgga
    19201 tcagacttga cccgaccgca cctgatgctc ctagtatcat tacagtatct gtcggctgga
    19261 tttccgtcaa atggttgatt agattataag ccgtaattcc tggcgtgacc attccggctg
    19321 cttcttccca actcatttta tctggaattt ttgctacttt tgctgatggc aacacgactt
    19381 cttctccgta agtcccgccg actgcatgga cagctactcg atcacctaca tgaacagttg
    19441 tgacatctga accaatctct gtcacaatac cagcgccatc attacctggt acataaggga
    19501 atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa ggcaacatca taaggattca
    19561 cactgaatgc ttttatttct acacgaacat gtccagaacc gacttctctg ggatgagcat
    19621 cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt aattgcaaat cttcgcatgg
    19681 tcaccatctc cctttttctt taagtttacg tttatgcctt tattttgaca aaaaaaatgt
    19741 cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc ttttttcgcg taacgtgtta
    19801 aattatttat ctaaaagcgt aaagattacg atttacgcta ataaaaaata attggaggaa
    19861 tttgttgatg gctaaaaaat caaagattgc caaagcaaaa aaacagcaag aaacgattga
    19921 acgctatgct gctttacgcc aggaattaaa agcgcaaaaa gattacgaag gcttgcgtaa
    19981 acttccttta gatgctcgtc cagaacgatt gaaaaatcgt gatttaatcg atggacgccc
    20041 tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa ttccgtgaat tagcacataa
    20101 aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc aagtctggct gtaatgtaaa
    20161 ggaggagggc gcatggacca ctccttggat catcaaaaaa gatacgttcg cttcttctag
    20221 cagaaatgct ggtactagaa gcagacgtat ctttttttac gcacagctgg tcctttttta
    20281 tatgtaaagg ctgcatggtt acttcgctat atttatttag taacaggagt cagtgcttcg
    20341 atacccacac taattttcaa tgctcggtca acttctgcca taatgggtcg atccaaatgg
    20401 cagacctttt ctttcaatct tgatttatcg atcgttctga tctgctccaa caggataacg
    20461 gagtctcttt ctattcctgt tccagtagct ttgatcccaa tgtgagtcgg aagcttaggt
    20521 ttagccattt ttgccgttat ggccgcaaca atgatcgtcg ggctaaaatg gttacctaga
    20581 ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct ccgatcccac gactggagaa
    20641 agatcagcaa aataaatatc tcctcttttt accataggat cctccttatt cttgatattc
    20701 tcttggtatc cgctgtccta atccgcaagc cacttcatag tggatcgtac ctaattgatc
    20761 tgctatatcc tgcatagtga tttctttgtc tgcatttttg ccgataagcg tcacttttgt
    20821 tccgacagga aattcttgag gcaatcggat catcagttga tccatacata cacgcccaat
    20881 cgtttcacag tagttccctt ctaccaatag agaaaaaccc tgcatctttc gtggccagcc
    20941 atctgcataa ccgatcggta tcgttccgat ccactctgct tcaggagtga tatatgtttc
    21001 tccatagccg attccttcac ctgctgggag ttttttgact tgaatcaatt cagaaaccag
    21061 ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg tgcccagagg gattaagtcc
    21121 gtacattgct actccataac gaatcatatt gccgattgtt tcatcatgcc ataaagccgt
    21181 tgcactattg ctgacatgta catatcgagg aagttcggga agttttttca gcacttctat
    21241 aaagcgttct ttttgcagat tccaatatgt atcatctgcc tgatctgctg tagaaaaatg
    21301 ggtaaataaa ccttcccatt ctaatacacg actttctttg atgaatgcag ctgcctcttt
    21361 tacttcttca ggtgtacaaa atccgatacg acccatccca gtgtctgctt ttagatgaat
    21421 cgaaagaggc gtctctagct tacttttctc taaaacagca gctgctgctt gcaaccattc
    21481 ccgtgtacct gcagttactg ataaatcatg agcaacagct acagtcagtg cgtcgtaagg
    21541 aaccatattc aagataagga tgggctgagt aaatccagct tctctcaact cgactccttc
    21601 atctaaattt gaaacacaaa agcccgaagc tccacctgca actgctgcct cagctgtttc
    21661 aatagctcca tgaccgtatc cgttcgcttt tactactgca aacaattctt ttccttgcgg
    21721 aagtcttgca acttcatttg ctacattttc agttatagct tttttatgta tcactgcttt
    21781 cgttgggcgg tgccaagcga caaccatcgt ttttccttct ttctagtctt ccaaaataat
    21841 ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc catacattgc cttcatgagg
    21901 tgaacgcgtc acaacaggct gaccattttc attttttaaa atctcgacat cttgaaaggt
    21961 aacttttccg atcccagttc cccaagcttt agagaaagcc tctttgcaag cgtaacgccc
    22021 gcctaaaaat tctacttggc gatgaaaagg caaggactgg aatagtttca tttcatcaga
    22081 ggtcaagatt cgtgcaatga attttggttt ttcttcgatc agccgagtga tcctaggaag
    22141 ttcgactgca tcgatcccaa ttccttttat catatgcttt tctcttttca ataaatttag
    22201 aacactctca ttctaccaaa ttataaccat tttgccacaa ataagtccac aaataacaaa
    22261 aatgaaagtt tctcttaaaa aagagaaact ttcattttat gtttaatcgt tatttgaacg
    22321 gattacaaat ccgcgttttt tatcattgtg acgtttaccg ccatcttttt ggctgttatt
    22381 atttttgcta taaccgcctt ttgatttgtt tccacgataa ttaccgccgt cacgacgatt
    22441 acggttgttt ccgccaccac gtttgccatt tttattgtat ccttttttct gcattggcaa
    22501 tgggcgttct ggtgtgatct tcactggcac tgcatcagct ggatcttttg ctgtcgtttt
    22561 tagcaaaagt gcaactaaat cttgtgcaga atattcttct aataatttat ctgctgtttg
    22621 caggtattta tctagaccat tttctgcaag ttttgtttcg atttgttcaa ctgcagcacc
    22681 taattgacct ttgaatgctt ctttttcggt tggtggacgt aaagtcgtca tccgtttttt
    22741 cgtcaagttt tcaatcacat gcaagtagtc catttcattt ggtgtaacga aagtcacaga
    22801 cataccgcct tttccggcac ggcctgtacg tccgatacgg tgaacgtagc tttctggatc
    22861 ttgagggata tcatagttgt acacatgagt gacacctgaa atatctaatc cacgtgcagc
    22921 aacgtctgtc gctacaagga tatctaaatg accgctctta aatgaacgca atacgctcat
    22981 tcgtttttgt tgagacaaat cgccatggat tccttctgca cggtaaccgc gtgcttctaa
    23041 tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg aagacgattg ttaattcagg
    23101 tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt tcgtattctt ttgcgcggac
    23161 atagtattga tcgatcaagt cagctgtcat ttctttcgct ttgatcttca catgatgtgg
    23221 atttttcatg aatttaacgc caatattctt gatagctggc ggcatcgttg ctgagaaaag
    23281 taatgtttgt ctttgatcag gaacttgtga aatgattttt tcgatatctt ccaagaagcc
    23341 catattcaac atttcatctg cttcatccaa aactaatgtt tgaacggttc caagtttcaa
    23401 tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta cccacaacga tatgtggacg
    23461 atctttcaag ccacggatct gacggccaat atctgctccg ccgtaaacgg cttgtacacg
    23521 tacttttttg tcgcgcccta aacgatataa ttcttcttgt gtttggatag ctaattcacg
    23581 tgttggtgcg ataactaaac cttgtaattc gtgacgatct ggatcgattt tttctaacat
    23641 tggtaatcca aatgcagctg ttttccctgt acctgtctgt gcttgtccaa taacgtcttt
    23701 tccagctaat gctagtggaa tcgtttctgc ttggataggc gttgcttctt caaaaccagc
    23761 gcgttcgact gatttcaaca attctggtga taattctagt tctttaaatt tcaaatgaat
    23821 cctcctatat ggttgttaac tgaggatgta gcttttttgc taccccgtcg atttgttcgg
    23881 tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt ttttatccat tcttagcccc
    23941 tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat tagaaaagcc aaacgggtcg
    24001 cgttcagctt tatcattatt atgacatctg gcttcaacaa gttgtttcta tggcaaaacc
    24061 agtccaattt ctcttcatct ttcggcaata ggttattcta acatagtttt tcgttttctg
    24121 caagtttaca gatttttatt tcatttgctg aagttgttct accacttcaa ataagcccat
    24181 gctgttgctg ccttttagca taatactatc tgttgcttca agattttgtt tgatagcatc
    24241 aatcaattta ttcttttctt tttcaaaata gaacacaggt agttgggggt atttttggtt
    24301 caattcttct tttagtgcat gcatttgttc cccataaagg aaaatcatgg cgaatttctc
    24361 aggatcgata tgttcactca tttgcgcatg catatttaaa gactctttcc ctaattccag
    24421 catatctgct aacacagcga ttctgcgtcc ttttgttggc agatttttaa aagtatctaa
    24481 taccagtccc atggctgttg gatttgcatt gtagacatcg ctcaaaatag ctgcaccatt
    24541 tcctgccgtt agccattcgg ttctgttttg agtgatagaa acatgggcga gcccttggcg
    24601 tatctcaggt atggacagtc caaagaagcg cccgatcgta tacgcaatca aagcattcgt
    24661 tacattataa cttccaggca gcgggatcgt aaatgtttct ccttctacta caaaatcggt
    24721 ttgtgctttt ccttttgctg tcacttccgc atgaacatcg ccatttccaa taccgaatgt
    24781 tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc aacggttcat ctgcgggcac
    24841 aacgagtaat ccgtctgata caagtccgtc agtgatttcc attttggctt tagcgattcc
    24901 ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc attgtgattg ctgcagcttt
    24961 tggttgagcc ataagtgaaa gttccgtgat ttcattggcg tgatccatgc ccatttccaa
    25021 aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg taaggaagac caatattatt
    25081 gttgtaattt ccttgcgtct tatatgtttt gtatttttca gacaagacag acgcggttag
    25141 atctttcgtg gtggttttac cattgcttcc tgtaattgca acgacttctg cgcctacttt
    25201 ttccaagtaa taggttgcca attgctgcat ggctttcaaa ggatctgcta caggaataac
    25261 tggaaaattt tttggagctt gttcaacagg caagctccaa aatgaagcag atgctccgtt
    25321 tgtgatcgct tgctggataa attcatgtcc atctcgcgta ccagcaagag gaacgaataa
    25381 acttccttcg gttatcttcc ggctgtcaaa ttcgacattg gtaatctttg tcagtgcaga
    25441 gcattcgctg cccacggctt gtgcaatttc ttgtattgtt agattcattg agatctctcc
    25501 taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg gttcgcatga ctctcacttc
    25561 caagtaaatt tctctattcg tatttagaag ctagaatgaa cgcggttacg caatcactcc
    25621 agcctcttct ttatttctct ttattcattt ttttcaaaaa aagattgacg ctgatggtat
    25681 cgattcattc ctaactggat cagttcttca atcaaatcac cgtatttcaa gcccatattt
    25741 tcccataaga gtgggtacat actgaactcc gtaaatcctg gcatagagtt taattcattc
    25801 aggaataatt catttttatt tgtcaaaaag aaatcgcacc ggctcaatcc gcttccacct
    25861 aacatcgtgt aagctaactt cgcgtactct tgcgcttttt gataaacttc ttctggcact
    25921 tcggctggaa tctgcatttc gattttatta ttgatatatt ttgcttcata atcatagaat
    25981 gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa cgtcttcatt tcctaataca
    26041 gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga ttgctcgaga atcatactga
    26101 taggctgttg ctaaagcatt ttgcagctct tctcggtttt ctgcttttgt aatgccgaca
    26161 ctagaaccca tattcgccgg tttgacaaac atcggataaa gcaaagaacc ttcacattga
    26221 tcaaatactt ttttaggatt ttctttccat tgattcttaa gtactggtac ataaggaact
    26281 tgcggcacac cagcagcttg taaaatatac ttggtcatga ttttatccat ggcacatgca
    26341 ctggtcaata cgcctgcgcc gacataaggc atattcaatg tctctaagaa gccttggatc
    26401 gttccatctt ccccgtttgg cccatgtaaa actggaaaaa cgatggctcc ttcttctttg
    26461 atttcgcccg gattgatcac ttttcctgta aagccttcct ctgtctgtcc acttgggtcc
    26521 catgaaagat gcaagacatc tttgctggca ggtttttctg ttagtaatgg acctttgacc
    26581 cattgtcctt cttttgtaat aaatacgagt tgaacttggt aataattata ataaatggca
    26641 tttaaaactg aaaatgcgga aagaatggac acttcatgct ctgcgctgcg tccgccatat
    26701 agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa atcgttcagt gatttactct
    26761 catattttag cacaaaacaa aaataaacga ataacttatt tataaaaaaa cgagccagct
    26821 tctaaaagaa cgagcgtcat ttattttttg tcagctagat tatttgtccc gttttttata
    26881 aaaagttatg ataaagaaaa ggagggatga tgatggcgca aacaaatcca cgcagccgct
    26941 tggcagaaat cattcgagtg ttcatcaatt acaatgtagt gccaaacttt gtacagcaaa
    27001 aaaatcccga acaggtaaaa aaagcctttg aagaactagg ccccaccttc atcaagatag
    27061 gacagatgct ttctgttcga gaagatttgc tttcttctgc cttcactcaa acattcaaaa
    27121 cattacaaga tagtgtacct agcgacacat tttcaacggt caaaaagacg atcgaaacag
    27181 aactttctct ttcattatca gatatattcg atgatttttc aaaatcgcct tttgcctctg
    27241 cttctatggg gcaggctcat cgtgccaagc tgaagaacgg tgacgcggta gtcgttaaga
    27301 tccaacaccc taacatcgcc gaagagatcc gacttgatct tcaactattt gaacgagcga
    27361 tccctttgat caaatacatt cctgaaacta gcgtagttga tctaaaaggg gtactccaag
    27421 aagtaaaacg ttccttgatc aatgaaatgg acttcttaaa agaaagtcaa aatggcgaac
    27481 aattctatca aaagaacaat ggctggaaag agatccgcag ccccaagatt tatgacgctt
    27541 tttgttcgaa aaaagtgatc gtgatggaag aaatgtctgg taaaaacttg aatcatttaa
    27601 tgaacgccga aaacaaaact gaaactttta tcactggaat tcaaaacaag cagctgaaac
    27661 aagaagttgc aaagctttta gttgagaact ttatgaaaca ggtgtttgat gatggttttt
    27721 tccatgctga tcctcatcca gggaatctgc tattccatgt actaacaaaa gaagaacaga
    27781 cacaagctag ccgaaaaact gaaacagtcc atgaaaaaga gttcggttcc tttgcttttc
    27841 gagcctccac ttctgcagaa gaccctgttg cgccatatac catcaactac atcgattttg
    27901 gtatgatggg gcatctttct gcaggactta ggcaaaaact cacacaagcc gttttagcgc
    27961 tgtatacgaa agatgcttat cgaatcgaaa aagcggtcct tcgactttgt cagcaagaag
    28021 gctcgtttga tgaaagtcgg ttccaccaag aactgacaag ttttcttgaa cagtattatg
    28081 attctcctat tgatgaaatc aacttgcaag aagtattcac acatgtcgtg acgatttgtc
    28141 atcaaaataa tctgcagttc gatcgtgata tcacgctttt gctgaaagca tttgggacat
    28201 tagaaggggt gatccgcgtc ctcgatcctg aagtttccct catggaagtc gcttcgcctt
    28261 ttgctcaaca ttattttctc actcatctgg atgtagaaga tacattgaaa caaagcggat
    28321 tagacttatt ggaagggatg aaggctgcgc ctaagatccc tcaacagctc caccatctat
    28381 tagaaatgtg gacttcggga caaggaaaag tcaatttgga attgaaaaag caagacaaat
    28441 tattgagcag gatcgaaagt atgatcaatc ggcttgtttt cggtatgatt ttagctgcat
    28501 tgatcgtagg gtcctccctg cttgtccaag cagcacctgt agaaaacgca gaggtcgttt
    28561 ccttattagg gatatttacg tatgctattg ccgcattcgt cattattttt ttggcaatcg
    28621 atgcattgat ccaaatgtat aagaagagga aaaagtagaa aaaggttgtg acagaaagaa
    28681 tcagcttcaa gaaataagaa ggggttttac ataaattgtt tctcaaattt ttgtgaaaat
    28741 tcggcttatt tttcgaagag gctacttctg aaacaccgag aataaaaaat gtgaaaaagc
    28801 tgttctgcac ccagtaataa gaacaatcaa aaagggaaca tggaacacat ttatcctgtt
    28861 tccctgttcc ctttttgatg tattcgagca taaataaaat accttcgtta cctactatct
    28921 tctgtacttg gtaaacctta gtcaattgcc ttttttaacg cttgacgaaa agacatatga
    28981 tctttctccg taaagggttt tggacccttt gtccttatgc cagcttttct taactttcca
    29041 ctatgataac gttgttctag aagcgtgccg atattcgctt cgtcaaattc ttttccagag
    29101 tgatggaaca ctcgcacacc cttcggcaat acaagtgaag ctaaaccata atcttgcgtg
    29161 atcaaaaagt cattcttctt aaccataccg acgattttat aatccgcact atcagctccg
    29221 cggtctacgt agacgatttg gacatgatta gcgtattctt tggtcgtata atggtccaca
    29281 ctggtcacga taaccacgtc aagaccatag gattctccta attttatgac atcttctttt
    29341 actggagaac catctccatc aatgaatagc ctcattcttt ttcgccttct tccataaatg
    29401 ataaacagtt actaatccgc tcagtaaaag accaacattg atccattttg ccaagataag
    29461 cagttgtaca ctttctatcg gcactgattc atggttcagc gtgatcaata atagatggat
    29521 tggctctccc atttcttttg ctaaaacaaa caaaagcgtc atcaataaaa actggtggat
    29581 cacactccct ttattttgag tagtatactt gacaacgatc cggcgatcca ttggaatagc
    29641 tgtatcagtt tgataatatt ccttatttag ccagctgcct gttactacgt gccagaaact
    29701 aagcagaaag acacctatcg caaaaccatt tggaatcaag aggaccaata aagaagaact
    29761 cagtccaagc aattgcatag ccaatggaga ccatgttaaa cgtttcaaaa tccatggtcc
    29821 gactattttt gctgcaatca agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt
    29881 ttctaatccg tacagtatgc caacataaaa actgctaaat aaaaaaagga aattccctag
    29941 cattccattt agaaaagtaa acagatttag ccagcttggc aacttccatt ccttcttctg
    30001 gtgtcctaaa acaaacacga acaagatgaa cagcagcaag aaaccataaa tagcaagatc
    30061 aaaaagcctt tcatcgaata acaatcgggc atttctcaaa agaaacaata agccaaagaa
    30121 tatcaaaaat aaaacaagct ctcgcgtagc taccagattt cttttgatgt ctttgaaatc
    30181 cagttcgtaa cgcggataat gactaaccgt atggtaggcc atgacgtaaa aaagtgtata
    30241 aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag
    30301 aaatagccag atagctgcaa ataagtactt tttcccatcc atattgataa aaccttgcgt
    30361 cttttcgtga aaattgaccg tggtattagc aggtacaagc catgctgcac tcagccctag
    30421 caaaacactt gaaaaataaa ataaaggaaa ataaaatacg cctaatattc caagcaatga
    30481 accagcacca ccaatcagca gagcgatcat cagcaacgtg taactatcta gaccaaatcg
    30541 tagactgttg actaaaaata gtccagtcat ccgaaaggca taaaacagcg taaatggcaa
    30601 aatcgtcagc caattatgcg agtctgttag tcccaaaaat aaaagataag gaataaaaat
    30661 aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag
    30721 tttttcgaac atgacaatgg ctccttaaac agtcttataa ctaatctgct cttcgatttt
    30781 agcattaaag gggaaataaa ccaattttac agaatatatt cttgtaaaaa aaaatacaga
    30841 aagaagcata agcttctttc tgcattttta cggtacttga ttactcaagc agacaagccc
    30901 ttctccacaa aagtcttgcc cgacacccct gcatccggca ggtgataaag atagaaatca
    30961 attatcctgt gcctccaaaa tcaattagga gctctgtaaa ccagagtcct tctcgacccg
    31021 tcgcataaat ttactttaac acagttccta tgtttatgcg agagcttttt tcattttata
    31081 atcgaccatt tgaaatcctt gaccgatatc tgaaataagt tcttctgtaa tctcaaatcc
    31141 ttcatgctta taaacctcga ttgcttgatg attcccctga ttcactcgca aaaagatctc
    31201 tctttccttt gtttgcgcag caagttcttc ataccagcga aaaactgaag aagtcaatcc
    31261 ttgaccacgt actttattta gcaaatataa tttacttaaa taaatttttc catttatctc
    31321 ttcataagca gtgtaaccga cagtcttttc ttcgcttttt aataaaaagt aggacacacc
    31381 ttctgtcagt tctttttgaa tctgtttttt tgactgataa gttttcagca tgtaatccac
    31441 ttgtttttga ccaatgatcg gcacaaaaac ttccggccat acttcttgta tgactgccat
    31501 cagctcatcc agttcatttt ctgttacttt tattttttcc atacacccac gtcctttttt
    31561 acattataaa tgaccttgcg cttgatggaa taaaaagagc atgatcatgc tatacacacc
    31621 caccgaccac ggtttcaaga aaatcacaat ctgcataaat tccctaaaag ataatttcgt
    31681 caaaccagct actagacaga tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac
    31741 tagtactttt ttaatatctt tcgtttttct tgtcagcaat tcatcaaatt tctgatactt
    31801 ttttggcgat aaaataagct ggacaaaggc tacgccatag taacggacaa gcaaaaatcc
    31861 gattgcttcc ccaatcacta ggccaataca actatataaa agcccaaatc cattgccaaa
    31921 catcagcatg cccgctactg aggagatccc tcccggcaaa ataggaatga tcacttggat
    31981 cacttgcaac agaacaaata tcatcactgc atacttgcca aattgctgga taaacgcttg
    32041 caaagacgtg ctcgatcgaa aaatcccatg atgataagca taaagaatca acacaaagaa
    32101 aaaaaacaga ccaataatgg gcatgatctg aagacatttt tgtaaaactg atcctttttt
    32161 agacatctat attccccaca tcttttttca tttattaaaa ctgcctcct
//
LOCUS       DAYYDS010000025        32178 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000025 DAYYDS010000000
VERSION     DAYYDS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.025"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(407..766)
                     /locus_tag="ACHA02_001124"
     CDS             complement(407..766)
                     /locus_tag="ACHA02_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8669486.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ACHA02_001125"
     CDS             complement(796..1137)
                     /locus_tag="ACHA02_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669487.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ACHA02_001126"
     CDS             complement(1134..1808)
                     /locus_tag="ACHA02_001126"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8669488.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACHA02_001127"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACHA02_001127"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8669489.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ACHA02_001128"
     CDS             complement(4322..5512)
                     /locus_tag="ACHA02_001128"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8669490.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ACHA02_001129"
     CDS             complement(5533..6060)
                     /locus_tag="ACHA02_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8669491.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ACHA02_001130"
     CDS             complement(6107..8896)
                     /locus_tag="ACHA02_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8669492.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ACHA02_001131"
     CDS             complement(9043..9243)
                     /locus_tag="ACHA02_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8669493.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ACHA02_001132"
     CDS             complement(9696..10016)
                     /locus_tag="ACHA02_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669494.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="ACHA02_001133"
     CDS             complement(10519..11637)
                     /locus_tag="ACHA02_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669495.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11870..11992
                     /locus_tag="ACHA02_001134"
     CDS             11870..11992
                     /locus_tag="ACHA02_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669496.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            12108..13415
                     /locus_tag="ACHA02_001135"
     CDS             12108..13415
                     /locus_tag="ACHA02_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8669497.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ACHA02_001136"
     CDS             13536..14735
                     /locus_tag="ACHA02_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8669498.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ACHA02_001137"
     CDS             complement(14762..16030)
                     /locus_tag="ACHA02_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8669499.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(16086..16152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16200..16841)
                     /locus_tag="ACHA02_001138"
     CDS             complement(16200..16841)
                     /locus_tag="ACHA02_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8669500.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ACHA02_001139"
     CDS             complement(17250..17753)
                     /locus_tag="ACHA02_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8669501.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ACHA02_001140"
     CDS             complement(17876..18640)
                     /locus_tag="ACHA02_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8669502.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ACHA02_001141"
     CDS             18748..19023
                     /locus_tag="ACHA02_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8669503.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="ACHA02_001142"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="ACHA02_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8669504.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="ACHA02_001143"
     CDS             complement(20579..20980)
                     /locus_tag="ACHA02_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8669505.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="ACHA02_001144"
     CDS             complement(20994..21803)
                     /locus_tag="ACHA02_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8669506.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="ACHA02_001145"
     CDS             complement(21790..22680)
                     /locus_tag="ACHA02_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8669507.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="ACHA02_001146"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="ACHA02_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="ACHA02_001147"
     CDS             complement(23348..24289)
                     /locus_tag="ACHA02_001147"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM8669508.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACHA02_001148"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACHA02_001148"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8669509.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="ACHA02_001149"
     CDS             complement(25274..26470)
                     /locus_tag="ACHA02_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8669510.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="ACHA02_001150"
     CDS             complement(26488..27216)
                     /locus_tag="ACHA02_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8669511.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACHA02_001151"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACHA02_001151"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8669512.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="ACHA02_001152"
     CDS             complement(28628..29086)
                     /locus_tag="ACHA02_001152"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8669513.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="ACHA02_001153"
     CDS             complement(29083..30546)
                     /locus_tag="ACHA02_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8669514.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACHA02_001154"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACHA02_001154"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8669515.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="ACHA02_001155"
     CDS             31602..>32178
                     /locus_tag="ACHA02_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8669516.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6921 c         5511 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DAYYDS010000026        31616 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000026 DAYYDS010000000
VERSION     DAYYDS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.026"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..617)
                     /locus_tag="ACHA02_001156"
     CDS             complement(<1..617)
                     /locus_tag="ACHA02_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8669517.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELV"
     gene            complement(693..839)
                     /locus_tag="ACHA02_001157"
     CDS             complement(693..839)
                     /locus_tag="ACHA02_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFM8669518.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(943..1254)
                     /locus_tag="ACHA02_001158"
     CDS             complement(943..1254)
                     /locus_tag="ACHA02_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8669519.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(1256..1453)
                     /locus_tag="ACHA02_001159"
     CDS             complement(1256..1453)
                     /locus_tag="ACHA02_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8669520.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1847..3574)
                     /locus_tag="ACHA02_001160"
     CDS             complement(1847..3574)
                     /locus_tag="ACHA02_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8669521.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(3567..4760)
                     /locus_tag="ACHA02_001161"
     CDS             complement(3567..4760)
                     /locus_tag="ACHA02_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8669522.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4947..5591)
                     /locus_tag="ACHA02_001162"
     CDS             complement(4947..5591)
                     /locus_tag="ACHA02_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8669523.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(5685..5819)
                     /locus_tag="ACHA02_001163"
     CDS             complement(5685..5819)
                     /locus_tag="ACHA02_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8669524.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5821..7953)
                     /gene="feoB"
                     /locus_tag="ACHA02_001164"
     CDS             complement(5821..7953)
                     /gene="feoB"
                     /locus_tag="ACHA02_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8669525.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7950..8423)
                     /locus_tag="ACHA02_001165"
     CDS             complement(7950..8423)
                     /locus_tag="ACHA02_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8669526.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8824..9048
                     /gene="nrdH"
                     /locus_tag="ACHA02_001166"
     CDS             8824..9048
                     /gene="nrdH"
                     /locus_tag="ACHA02_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8669527.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            9207..11366
                     /gene="nrdE"
                     /locus_tag="ACHA02_001167"
     CDS             9207..11366
                     /gene="nrdE"
                     /locus_tag="ACHA02_001167"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8669528.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            11416..12381
                     /gene="nrdF"
                     /locus_tag="ACHA02_001168"
     CDS             11416..12381
                     /gene="nrdF"
                     /locus_tag="ACHA02_001168"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8669529.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(12470..13609)
                     /locus_tag="ACHA02_001169"
     CDS             complement(12470..13609)
                     /locus_tag="ACHA02_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8669530.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13918..14631)
                     /locus_tag="ACHA02_001170"
     CDS             complement(13918..14631)
                     /locus_tag="ACHA02_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8669531.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14885..15586)
                     /gene="nagB"
                     /locus_tag="ACHA02_001171"
     CDS             complement(14885..15586)
                     /gene="nagB"
                     /locus_tag="ACHA02_001171"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8669532.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15820..17352
                     /locus_tag="ACHA02_001172"
     CDS             15820..17352
                     /locus_tag="ACHA02_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8669533.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(17543..18745)
                     /locus_tag="ACHA02_001173"
     CDS             complement(17543..18745)
                     /locus_tag="ACHA02_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8669534.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(18827..19516)
                     /locus_tag="ACHA02_001174"
     CDS             complement(18827..19516)
                     /locus_tag="ACHA02_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8669535.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(19622..19990)
                     /gene="rplL"
                     /locus_tag="ACHA02_001175"
     CDS             complement(19622..19990)
                     /gene="rplL"
                     /locus_tag="ACHA02_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8669536.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(20043..20546)
                     /gene="rplJ"
                     /locus_tag="ACHA02_001176"
     CDS             complement(20043..20546)
                     /gene="rplJ"
                     /locus_tag="ACHA02_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8669537.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(20750..20877)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20956..21645)
                     /gene="rplA"
                     /locus_tag="ACHA02_001177"
     CDS             complement(20956..21645)
                     /gene="rplA"
                     /locus_tag="ACHA02_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8669538.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(21749..22171)
                     /gene="rplK"
                     /locus_tag="ACHA02_001178"
     CDS             complement(21749..22171)
                     /gene="rplK"
                     /locus_tag="ACHA02_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8669539.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            22514..23182
                     /gene="sdaAB"
                     /locus_tag="ACHA02_001179"
     CDS             22514..23182
                     /gene="sdaAB"
                     /locus_tag="ACHA02_001179"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8669540.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            23195..24067
                     /gene="sdaAA"
                     /locus_tag="ACHA02_001180"
     CDS             23195..24067
                     /gene="sdaAA"
                     /locus_tag="ACHA02_001180"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8669541.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(24158..25258)
                     /locus_tag="ACHA02_001181"
                     /pseudo
     CDS             complement(24158..25258)
                     /locus_tag="ACHA02_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(25350..26237)
                     /locus_tag="ACHA02_001182"
     CDS             complement(25350..26237)
                     /locus_tag="ACHA02_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8669542.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(26314..26859)
                     /gene="nusG"
                     /locus_tag="ACHA02_001183"
     CDS             complement(26314..26859)
                     /gene="nusG"
                     /locus_tag="ACHA02_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8669543.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26960..27130)
                     /gene="secE"
                     /locus_tag="ACHA02_001184"
     CDS             complement(26960..27130)
                     /gene="secE"
                     /locus_tag="ACHA02_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8669544.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(27154..27306)
                     /gene="rpmG"
                     /locus_tag="ACHA02_001185"
     CDS             complement(27154..27306)
                     /gene="rpmG"
                     /locus_tag="ACHA02_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8669545.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            27468..28112
                     /gene="cbpA"
                     /locus_tag="ACHA02_001186"
     CDS             27468..28112
                     /gene="cbpA"
                     /locus_tag="ACHA02_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8669546.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(28168..28671)
                     /locus_tag="ACHA02_001187"
     CDS             complement(28168..28671)
                     /locus_tag="ACHA02_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8669547.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(28684..28730)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            29268..30233
                     /locus_tag="ACHA02_001188"
     CDS             29268..30233
                     /locus_tag="ACHA02_001188"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8669548.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(30332..31504)
                     /locus_tag="ACHA02_001189"
     CDS             complement(30332..31504)
                     /locus_tag="ACHA02_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8669549.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(31491..>31616)
                     /locus_tag="ACHA02_001190"
     CDS             complement(31491..>31616)
                     /locus_tag="ACHA02_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8669550.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9735 a         6444 c         5518 g         9919 t
ORIGIN      
        1 taaaccaatt cttttctttt caattgctgt tcatattgtt ctcgttgcct aatagttatt
       61 ctaataaata aaaatagaac aacaaacatt tgaatgatta aaaatattaa gacaccaaac
      121 acaaaatgat caaatacttg atattgatga gcaacataag aaacaaataa accaattgta
      181 agtaaataaa tcatgaatat agcagtaagt ttagaactgg actgttcaac aatcctattt
      241 atagctaatt ttctatataa ccaaataaac atcagcaaaa acaaagaatc aagcattatc
      301 tggaatacta cataaccata cccttttatc ccttcaaatg aaaacatata cgtcataacc
      361 atggaagtaa taatagatct gaaatcatca ataagcatac ataatagtaa tttatttaaa
      421 agcactatat tagttttctt cttttccttt aatataaaat aacttccgat tataggtata
      481 gctgcggaaa catcagtaaa taattcaaca aaaaaagtga gtggtatcaa taaacataca
      541 aaaagatatt ttcttttgtt aagatcagta ttagtcatat aaaataagag aaaaaagaaa
      601 aagtttccca cgaacattct taatagataa gcttttgtat cgatagtaat caagagatcc
      661 cctcctgatt ttaggatatc ataaaaaaaa ttttagttac actttttaaa taatgaaaaa
      721 acacattcca ctaaatttga tgccggtttt ccttgaggtt ttgttccagc acctccttta
      781 atttttttaa gctcttgatc tgaacattgt ttagcattta gtctattttt ttcttccatt
      841 acgattcccc ctacaaacta taaatacttt tatgtcactg attcaatata cctatttttc
      901 taatcaaaaa attttgagac atatttaata ttataagtat cattaaaatt gagatttatc
      961 tccataatct gctcgatata aaccatttat tccagcagtt cttccaattt cactcaaatt
     1021 tcttattaat tcctcttgat attcagtgaa tcttatttta ttagtataag cagtaaaata
     1081 tagataattc accaatctat ttattaaagg accgggatct aattcatatt tttgtttttc
     1141 taattttaaa tatacctttt gtaaaacctc aagaatatca gaatatttag gatctttact
     1201 tatagatata tcattatata attcatgaac aatttgctta gcattttttt tcattttagc
     1261 acttccctgg aattgctcca cctaaaaaac cacctataga cattcctgca atacaagtag
     1321 ttgccttggc ccaatcgacc gtacatttat ttttagtgca atacactcca tttccataat
     1381 attttccact atgagtggta cccccataga taagttgtgt ctctttaata gacaaaattt
     1441 ttaaatgttt cataatctaa tcacccctaa attaatgttt ttagactatt attctaacat
     1501 tcattaaatt tcaaaaaaca attccttctt gaaacgtagc aaaataattc ctgaataaga
     1561 atttaatcac ttttaagtat ataaactatg tatattcttt aatatttttt gagaatgcac
     1621 ttcgtacata ataaacttta atcacattag tctttcatac ctacgatcga tctctttgac
     1681 tcgcacccat aaattcataa tagtttgtcg gcgtctttgg acgtttcgaa gatgagctac
     1741 gcattctctt tttttataga caaaaaagat agagacgaac atggagcttc caaatttgga
     1801 tatttgatcc aattttggaa gctccattta tttcgtgtct gtctttttat tttttccagc
     1861 tttcgactaa ttcttggttc tctttgatcc agtcttttgc tgcttgctgt ggatcttttc
     1921 cattgttgat ttccaacata actttttcca tatctttttc tgaccaatgg aaattgtcta
     1981 atactttgta agcttctggc tggtcttctt tcaatccttt tcgaaccatc gtatggatct
     2041 gctcttcgct tcccattccg ttttcaggat cttctaagta tttcagatca tatttggcaa
     2101 acatccaatg aggtgtccat cctgtaacta caattggttc gtgttttttg atcgcttgac
     2161 ctagtgcaac ggtcattgct cctgaagaag atgtctctac tttccagtct ttcaagttgt
     2221 catatttttg gatcgtgttt tcagcagccg taactacacc agctcctggc tcgattccta
     2281 tgattttctt acctgcctga tcggttaact catcgatcga atttacatcc atgtaggctg
     2341 gcactgctaa accaactttt gctcctgtca aattggcacc taggtcttcg acttgatctt
     2401 tatattgtgc atattgtgaa ccatgagttt taggtagcca agctgaaacc atggcatctg
     2461 actcgccatt tgaaacagat ttccacatga ttgaattatc taatggcgtc atcgttacat
     2521 catagcccat ttgtttcaat acttcaccga ctacatttgt tgaagcaact tctgtatccc
     2581 attcaacata agataagttg atacgttttg tttctttggc agatgaaaaa ctccctatac
     2641 caatcgcacc taagatcact actactacag caccaatgat cagtccttga cgttttttac
     2701 ttttctgtgt tgttgctcct gcagcttttt tattgtttgg ctggttcaaa tgctgtgtaa
     2761 aacggtcaat gatgattgct aagataacta atgcaacccc gttgacaaat ccattcccga
     2821 cttgtgctct ttgcaatgct gataatacgc cgcgtcctaa gccgggtgca ccgatcatag
     2881 aggcaataac gaccattgaa agtgacagca ttgttgtttg attgatccct gccatgatcg
     2941 tacttttagc taaaggcaat tctagtttga atagtttttg acgacttgtg ctcccgaatg
     3001 aatcagaagc ttctactaat tctttaggaa cttgtcgaat ccctaaattc gtaaaacgaa
     3061 cggttggtgg cagagcaaag atgactgagg caaatacccc tggaaccatc ccgataccga
     3121 aaaatgcaac tgctggaatc aaataaacga atcctggcat tgtctgcata aagtcaagga
     3181 tcggtttgat aatactttgg gctttactgc tttttgccat caagatccct aatggtaccc
     3241 caatgatgat cgagatcaca ctagataaaa gaacaagtgt gacagtattc atcaaatcat
     3301 tccatagacc ttggttataa ataaaccata gaccgattaa tgtgaacaac gtaagtcctg
     3361 gacgtttctt agaaataaag aaagctgcta ccgtcaataa caagataaac aaaggagctg
     3421 gaatcactaa cagtaaattt gtaatccccg acatcactgc ctgtccaatc gtttgtaaaa
     3481 atgaaaatag gccagaaaaa gttgtcgtaa accattctgt gatattttct acccagtttg
     3541 ccactggtaa ttgatagttc aatagattat tcatgttcac tcacctcgct tgtttctgct
     3601 aatgcttcaa tcacactacc tcggatgacg acacctttta attttccatc ctctacaact
     3661 gcaagtggag tcggtgagtc ataaataata ttgaaaatat cacttaccac catgtctttc
     3721 ccaatcaccg tcacattttc atctaggaag tcgatcaatg gacgtttttg cttcctagct
     3781 tctaacgctt gatctgcagt gatgatccct ttcaggtgtc tctttctgtc aacagccatc
     3841 agcatgctga cttcttcatt acgcatcctt gtcaatgcaa cgtttggtcc atcagattcg
     3901 atattcgtcg tcaatgcagg taccatgatg ttttgagccg taagcacttt ggaacgatcg
     3961 acttcttcca caaattcacg tacataatca tttgctggat tggtcaatat ttcttcacct
     4021 gttccgatct gcatgatttc gccgtctttc atcaaggcga ttctgtcccc aatccgtaaa
     4081 gcttcattca aatcatgggt aatgaaaata atcgtttttt gaacattggc ttgcaggtcc
     4141 agtaattcgt cttgcatttc tcgccggatc agcggatcaa gagccgagaa cgcttcgtcc
     4201 atcagcaaga tctcaggatc atttgctaaa gctctggcta ggccaacccg ttgttgcatc
     4261 ccaccagata actgactagg atattggtct ttaaatgaaa gcaaactaga attttctaat
     4321 gctttttctg ctttttcttg tcgttcttct ttaggtacac cacggacttc taacccgtat
     4381 tctgtatttt ccaagatcgt tcgatgtgga aacagaccga agttttggaa aaccatattg
     4441 attttatgac gacggacttc tcgcaattct tctttgctta acttcgatac atcttctcca
     4501 tcaatataga tactgcccga agttggctca atcagacgat tcaatagacg gatcaatgtg
     4561 gatttcccac tcccggaaag ccccatgatc acgaagattt ctccttcttt tacgtcaaaa
     4621 ttcacgtcat acacaccaac cgttgcaccg gttttcttta aaatctccgt tttcggctgg
     4681 tgatcattca tcatatctaa agcggcttgt gtctttttac caaatatctt tgttaaatgt
     4741 tttacttcta cttttaccaa acaaattctc cttttttatg taaattcatt tttctttctg
     4801 aaaaaagtga ccacactatg ttaacatcat gtagtcactt tgtcaaaaat gaaagaaatt
     4861 tgcttgtcca aactgaaaaa aacttggcta aatctttgta aataaaaggt ttagccaagt
     4921 taatattttt ttaatatttc acacaatcat tctgcaagct gatgctccgg gtaaaaaaag
     4981 tttgtcatac gctgtagtgt atattcattt ccaataaaat gaacgatgtc ccctgccgta
     5041 aatttagcat aggggccagg tgaaacaagc agttcttcat tatgagaaat tgcgataact
     5101 gtggcgccag tcgcctgcca gatattcaac tcgctaattg aacggtccag atgcattgct
     5161 ttttctgata aagataattc aaacggaagt aaaggattca tcttatgtat tcgcttcgtt
     5221 tgattgacca gctgatccaa taattgagaa aaatgattca gctcatcttg ttgcttctcc
     5281 acacttgcca tgatttctgc ttttgtatct tggatggatt gtacatcttg atattgcaat
     5341 aggaaatctt ttgctttttc cttcgaagca acaaatgccc cacttccatg ccgtacttcc
     5401 atgattccaa gatctaccag cacattgata gccttgcggg tagtctcagg agaagcattg
     5461 tacgtactgg caagtgttga gcgggcatgg atcttctcac ccactttata tcggttttca
     5521 acgatacgtt cggctaaatc aacagcaatc tgctggtagc gcgggcggac gatttttttc
     5581 tttccagaca ttttctttcc tctttctttt tttactttcc ctcatcttac tatgctctag
     5641 caaaataagc aaaaaagggg agaatagcct cttgcaacga ctgatcatcg cttgatctgt
     5701 tcctttttta ctgggcaagc tgtttgacag tcatcacaat tcttaccgga ttttaaacga
     5761 gtatagacaa ttgcgcctgc acttccaaag attaacacac ttaataagaa tgtcgccatc
     5821 ctattcctct ccttctaatg acaatatagt ataaaactgg ttcttggatt ttggctgacg
     5881 gaacaaaaca taaacgagtc ctatcatcag taaaatagca agatacgtcc ctaacgctac
     5941 ctgtccaccc tccaataagt gtcccaactg ataaatcaca aaactaacta aataagctaa
     6001 accgcattga tagcctatgg cgatccaagt ccatttcttg gcattcattt cccgatggat
     6061 agccccaatt gccgcaaaac aaggcgcaca aagtaaattg aaggctaaca atgaataagc
     6121 tgctgcggga gtatatgtgt gttgtagcac ttgccagatt tctctgccat tttcggatac
     6181 ttccgcatga ccaaacaaaa taccaaatgt gccaataata ttttctttag cgaccaatcc
     6241 tgtaatcgct gcgactgttc cttgccagtt tccccaacca agcggctgaa aaacaactgc
     6301 caggattcgc ccgaaaaagg ctaagatact ttggtcttca gaaacaggtt gagcataaaa
     6361 attataagaa gagctgaacc agatcacgat actagaaaca aagataatcg ttcctgcttt
     6421 tttaacaaaa gacttgctgc ggtcataggt ttgtcttagg accgctgacc attgaggaaa
     6481 atgatatgcg ggcaattcca tgataaaagg agctgtatct ccagcaaaca aacgtgtttt
     6541 tttcaagctg attccagaga aaataattgc agtcatacca agaaaatagg cagaaggagc
     6601 tacccaagat tgatgaggaa aaaaggcccc tgctactaag gcaataatcg gcaactttgc
     6661 agaacaaggc atgaaggttg tgaccatgat cgtcaaacga cgatcttgct cgttttcaat
     6721 cgtgcgactg gccatgacgc ctggcactcc acatcctgta gcaatcagca taggaatgaa
     6781 ggattttcct gaaagaccga atttcctgaa aagtcgatcc atgacaaaag cgatcctcgc
     6841 catatatccg cagtcttcca gaaagctcaa gcataaaaac aatataatca attgcggtag
     6901 aaatcctaac actgctccca cgcctgcgat gatgccgttc aagatcagct cctgcatcca
     6961 tcccgctact tgccattcag ctaacaatct gccaacatgt tctggtaacc atgtaccaaa
     7021 caatgtgtca ttgatccaat ctgtccccat tgttcctact gtttgaatag ccagataata
     7081 gatcagccac atcacaaatg cgaagatcgg caaggctaac caacgattcg ttgcaatctg
     7141 atcgattttg tcactcatcg ataattggaa agaatcattt gacacaacac agagtgtaca
     7201 tagttgggta ataaattcat aacgttcatt gaccagaatc gtctcactat cgtcgtccaa
     7261 tagtttctct gttaactgga tcagttgctc gatctctttt tgctgagaaa ctgctatctc
     7321 gtaatcctct cttaccttct gatctcgttc aaaatatttc attgcatacc agcgtttctt
     7381 gccgctttgt tctcctagta catcttcaat ttcatgtaaa gctgcttcta aacgtggatc
     7441 atatgtacag ggaacaggaa tagaaggccg cttttttgca gactcctgaa tcttttcgat
     7501 tagtttttcg attccttgct ttttcaatac gctgattggt aaaactggta caccaagacc
     7561 ataggaaagc ttttctagat tgattttttt tccttctttt ttgatgacat ccatcatatt
     7621 cagaccaatg atcataggaa tgtctaaatc caataactgc gtagttaaat aaagatttcg
     7681 ttctaaattg gtagcatcaa taatgttgat gatcagttca ggctgttcct gaaccaaata
     7741 atctcttgca atcacttctt ctggtgtata aggagacaaa gaatagatgc cgggtaggtc
     7801 ctgaatatga atgttcggat tttttttcaa cgatccttct tttttttcta ctgttacgcc
     7861 cggccaattc cctacatatt gattcgaacc agtcaagaga ttgaacaagc tagttttccc
     7921 gctgtttgga tttccgacta acgcgatatt cattcttttt cctccctttg tatcaagatt
     7981 agttcggcct ctgactttcg taacgtcagt tcataccctc gcactgtcag ttcgattgga
     8041 tctcccagag gggctagttt tcggataact atttttacat ttcgcgtcaa tcccatatcc
     8101 attaaccgtc gcttcactgc gccttgtccg tggattgcta cgacgatccc agtctctcct
     8161 gcttttaatt gatcaagaga agtccagctt tcagaggaag cagtcttttt attgacaaag
     8221 atttgaccta gtatttcccg acttactgcc actcgatgac tttttaccag taaaatggca
     8281 ttgttgcctg ataaattgac gacgacgatt ttacttccgg caacgatccc gatattttgc
     8341 aaatggcgtt ttgttttttc ttctgtttcg attcgttcga cttgataaac gaaattctgc
     8401 tttgcctgcg ctaaggtctg catattgatt cctctcttgt gaaataaatc atgtattaaa
     8461 atcattcatt atatgttcta attttcttac gtgttacgca aataaatgag aatcattctc
     8521 acatagaata ctctcggtaa ttgagaatgt caattatact tgtgatatta tgagcgaatc
     8581 tttagtattg atagataaaa atgagaaaaa aatatttctt catagttttc cacaattttt
     8641 tctctattaa atttgctatt ttcccacacg atacttgttt tcagctcttt tttcaaaaac
     8701 acaaaactta gtattccaca gtttccacaa cacaatatgt agtgtttgtt gtttgacttt
     8761 tttactaaga gttttatact atctattata gataataaga atgtgttaga ggaggcactc
     8821 accatgaacg taaaaatctt ttctaaaaat aattgtatgc aatgtaagat ggtcaaacgg
     8881 tttttaacag aaaatcaaat tgcttttgaa gaagtcaata tcgatgaaca gcctgaagca
     8941 gtagactggt tgaaagcaca aggattccaa agtgttcctg tcattacttc tgatgcagaa
     9001 acagttatcg gattccgtcc tgatcagttg cgtaaacttg ccagctaaca tgactaaaag
     9061 aatcaaaacg tgatacatgg atttgaaggg cttgagacag caccagtttt tgtcgcaaac
     9121 ttccaaatcc ttttttaaag cttaaaaaca atgcaatgat ttgcaatcaa gcatcatcaa
     9181 atacgaaatg aaggttgggc gtacgtatga gtcttaaaaa tttacaagat gtcagttatt
     9241 tcaagctgaa taatgaaatc aatcggccag tcaatggtca aatccctttg aataaggata
     9301 aagaagcttt agttgccttt tttgaggaaa atgtcaaacc aaatacgatg acttttctat
     9361 ctattatgga aaaaatcaat tacttgatcg atcaagaata tatagaagca gaattcattc
     9421 aattgtattc accatcattt attgaaaagc tatatcaatt tttggacgag caacattttt
     9481 ctttcaaatc ttttatggct gcttacaaat tttattctca atatgcgttg aaaaccaacg
     9541 acggttccgc ttatctggaa acttatgaag atcgagtagc cttcaatgcg ctttactttg
     9601 cgaacgggga tgaagaatta gcacttgctc ttgcagacga aatgatccat caacgatatc
     9661 agccagctac accttctttc ttgaatgctg gacgtaaacg tcggggcgaa ttggtgtctt
     9721 gtttcttgac tcaagtcact gacgatatga actctatcgg gcgctcaatc aactctgctt
     9781 tgcaattgtc ccgtattggt ggcggcgtag ggattacttt atcaaatctt cgtgaagccg
     9841 gcgctcctat caaaggatat gagggtgctg caagtggtgt cgttccagtc atgaaattat
     9901 tcgaagatag tttcagctat tctaatcaat taggtcagcg tcaaggagct ggcgtggttt
     9961 atttgaacgt tttccatcca gacattattg ctttcctttc agcaaaaaaa gaaaatgcag
    10021 atgaaaagat tcgcgtaaaa actctttctc ttggcgtgat cgtacctgat aaattttatg
    10081 aattgacacg taaaaacgaa gatatgtatt tatttagtcc atatagtgtc gaaaaagaat
    10141 atggtgtccc attctcttat gtaaatatta cagctgagta tgatcatcta gttgcaaatc
    10201 caaacattcg caaacaaaaa atcaaagcgc gtgatcttga aaatgaaatc tcaaaattac
    10261 aacaagaatc tggctatcct tacgtaatca atattgatac agcgaaccgc gagaatccaa
    10321 tcgatggcaa gatcatcatg agtaatttat gttcagagat tttacaagtt caaacgcctt
    10381 ctgtgattaa cggcaaacag gaatatgaag tattaggaac ggatatctca tgtaacttag
    10441 gctcgacaaa tatcgtgaat ttgatgcaaa gccctaactt tggtcaatct gtgcgtacga
    10501 tgactcgtgc attgacattt attacagatg catctgatat cgatgtcgta ccaccgatcc
    10561 aaaacggaaa taagctgaat catacgattg gattaggtgc catgggctta cacacttatc
    10621 ttgcaaaaga acaaatggaa tatggctcag aggattcatt agactttaca aatatttact
    10681 tcatgctgtt gaattattgg acacttgttg aaagcaacaa tatcgctcgt gaaagaaaac
    10741 aagttttcca taattttgaa aaatcagctt atgctgacgg cacttatttc gaaaaatacg
    10801 taactggaga attccagcca aaatcagaaa aagtcaaagc attgtttgaa ggcattttta
    10861 tcccaactgc tgaagattgg aatgctttga aacaagcagt aatgaaagat ggactgtatc
    10921 atcaaaatcg cttggcagta gcaccaaatg gctcaatttc atatatcaac gatacgagtg
    10981 caagtattca tcctatcact cgcatgatcg aagaacgcca agagaagaaa atcggtaaaa
    11041 tttattaccc agcgccttat ctatccaatg agacgattcc gtattacact tctgcttatg
    11101 atatggacat gcgtaaagta atcgatgttt atgcaatggc acaacaacac gtcgatcaag
    11161 gcatgagttt gactttattc atgcgttctg aaatcccaga aggcatttat gaatggaaga
    11221 caacaacgaa gcaaacgaca cgtgatttga atattttaag acattacgct ttccacaaag
    11281 gaatcaaatc catttattac gtacgtacct ttactgatga tgcagaagaa atcggaagca
    11341 atcaatgtga aagctgcgtg atctaatatc atagatagat tcgcttattc atattttaat
    11401 taaaggagca ttctcatgtc agaaacatat tatgaagcaa taaactggaa tgaaattgaa
    11461 gatatcattg ataaatccac ttgggagaag ctgacagagc aattttggct agatacacgt
    11521 atcccacttt caaatgacct agatgattgg cgtacattgt ctcaattgga aaaagataca
    11581 gtaggacatg tatttggcgg tttgacactg ctagatactg tccaatctga aagtggcatg
    11641 gatcagttgc gtaaagatgt ccgcactccc catgaagaag ccgttttgaa caatattcaa
    11701 ttcatggaat ccgttcatgc caaaagttat tcttctattt ttagtacact gaatacgaaa
    11761 aaagaaatcg aagaaatttt tgactggaca aatacgaatc cttatctgca aaaaaaagct
    11821 gagcgtatca atgaaatcta caaacacggt acaccgctag aaaagaaaat tgccagcgtc
    11881 tttttagaaa cattcttgtt ctattcaggt ttttatactc ctctttatta tttaggaaac
    11941 aacaaattgg ctaacgtagc agagatcatc aaactaatca tccgagatga atctgtacat
    12001 ggaacctaca tcggttacaa attccaatta ggattcaacg agttacctga agcagaacaa
    12061 gaaacgttaa aagactggat gtacaatctt ctttatgagc tttacgaaaa cgaagaacgt
    12121 tatacagaag aattatacga tccaatcggc tggacagaag aagtcaagac attcctacgc
    12181 tataatgcca acaaagcatt gatgaaccta gggatggatc cactctttcc agatactgcc
    12241 aatgatgtca acccaatcgt aatgaacggg atttcaacag gcacaagcaa ccatgacttc
    12301 ttctcacaag tcggaaacgg ctatttgtta ggtcatgtag aagcgatgaa agacgacgat
    12361 tatttgattg gattggaata aggatttatt ttaattcaaa aatttttcga aaaataaaaa
    12421 gagtttagcg tacagatatt ttaaatgtac actgaactct tttttatgct tattcacttg
    12481 tttcttccag cacttttgga ctgtctaact tttccgaaac atgttccatt cttctcaata
    12541 aatagatttt ccggatattt aatattatcg tcttaatcac tgcatagaca ggtatgcaaa
    12601 tcagcatgcc taatattcct gcgacacttc ctgctccaat caataccaag atgatcgtca
    12661 aaggatggat attcattgat ttcccaaata acaacggctt gatcaaattt ccatctaatt
    12721 gttggatcac taaaatcgac aacgccatat acaaagcttg aaatggagag ataaacaaac
    12781 cgacaatgat tgctggtacc gctccaataa aagggccgac atacgggata atattcgtga
    12841 ttccgcaaat gaccgcaagc aacaagctat aaggctgatc gaatattctc attgccaaaa
    12901 agctcattac accaataatc aaagcatcta agatcgtact gctgatatag gcagataatg
    12961 tttcgtttaa ttcttttacc gtttggcgta actctttacg aatcgctttc ggaaagaagt
    13021 gagacaacgc atcaataaat ttgtgcccat ccttgaacat aaatagcaaa ataaatggaa
    13081 cggtaaataa caaaataaaa aattgcatca taaatgaaat gatttttgag acacttgcag
    13141 ataaactgac taatatgata ttgaacaaat tactaagaga taaatttgct gatgctaatt
    13201 gttcttccaa attgaaattt ttaaactctt cccgctgcgc caattcattt aaccatttgc
    13261 ttgtttcatc tgcatacatt ggcaagctct gggtcaattg aattgtctgt tccattaact
    13321 gaggcactac attcatgaca gttaaaacaa ctaaaatgat tacgaccaaa aacgtcaata
    13381 aaaaaccgaa cactctcggg atccgatgct tttcgagaaa aacaacgatt ggatcgaaca
    13441 tataatagat aaaaccagca acaagcaatg gtaaaaaaac tgccgaaagc attttcaata
    13501 ttggactaaa aatattcggc atttgtaaaa taaagtacac gccgatgatc gtgagtacta
    13561 gctccactgt ccagaaaaat aattttgatt gtttaaaacg tgattgcata catctcctcc
    13621 aaggtccttt gatacttcta ttctatcatt taacaaagag ttacgcgctg agattttcat
    13681 ttgcttaaaa aaaacaaaaa aaataaacat attttaatga gtagacgagt acttaaacaa
    13741 aaacacggtt tggagtagtg acaaaaatta tactgcagtc cctttttcca aataaacggt
    13801 gttcaaaagc ttgcatcttc gtaataagcc cagccaaacg aaaaatataa ggaactattt
    13861 ttatttggct gggcttatta cttgatgtag gacatctatt ttacaacctc agatcattta
    13921 ttgcgcaata ctgactaaat gcaactttgt gatatttttg tgtacggaca actcctcgat
    13981 cacatcggca taagtgagcc cttttggaag atcaatcgta tagatatttt tgtacactcg
    14041 gtagtcatca acgaatttta cgtcaaaatt cacatcttcg attacgatat ttttctcttc
    14101 aaaataatcg gtaagaaaat ctttggtttc ttttctatgg ataaattgga tttctaactt
    14161 tttagtcgta ggtacatgga tcacgcgttt cactactgtg agtgcaatag caatcccgat
    14221 aaaactgatg atcgcaatgg aatagtaacc catgccgatg gcgatcccta atccagcaat
    14281 tgcccataaa gaagcggcag tcgtaagtcc cgtcaccgat tgtttcgtga cgatgatcgt
    14341 tcccgcgcct aagaaaccga caccactcac cacttgagca atcagccttg cttcatctga
    14401 acgaatcgtt ccagcaagct gtggatactc taaagcatct cttaatgcac cactagcgat
    14461 ctcaacttgg atcaatgcaa tgatacatgc tcccatacaa actaaaatat gcgtacgcat
    14521 acctgctgga cgatttttat attgtcgttc aaaaccgatt gctccgccaa atagcatagc
    14581 gatccctaaa cgtaaaataa tctctggaat tgtcaatacc atatcattca tctaaacacc
    14641 ctccagtcca atatatcatg tctgaaagga gcagacaaaa aaagtgcaat ttttacctct
    14701 tacgaaaaaa ggactgtgac aaaatttttg tcatagtcct ttttatgaat aaacggtgtt
    14761 caatcagctg accctcggta ttactcaaca aacggcaaaa tatggggagc tatttttcct
    14821 ttgtcttctc taatactcag gtcaaaacgc tgatttcaca acctctttag cctactattg
    14881 aaaattacaa cttacttgct gcttcttcat cgatgatcac aacaacattg tcgtgatttt
    14941 gcaatacact tgctggcaaa tgattcgtta caggtccttc aatcatccct ttgattgcat
    15001 cagctttgct ttcgccaaat gccatcaaga cgatttcttt acttttcata attgaaccga
    15061 ttcccattga aacagctttt gttggtacat cttctgcttt atcgaagaat cgtttgtttg
    15121 cattgattgt tgattctgtt aagtcaacta ctgaagtcgt tccgtcaaat gaagcaccag
    15181 gttcattgaa accgatatgt ccattttgcc cgattcctaa gatttggata tcgattggat
    15241 gtgcatcgat gatttgatca tactttttgc atgctgcttc cagatcttct gcttttccat
    15301 ctggtacaaa cgtttctttg aatggttttt tatcaaataa ttggacatcc atgaaatgac
    15361 ggtagctttg ttcatctgaa ccgcctagtc caacgtactc atctaagttt acagaagtca
    15421 tttcactgaa atccaaatcg cttgaagtca tttctttata taaagtaact ggtgtgcttc
    15481 ctgttgccaa tcctaatact tttgcacctt tgttcatacc ttctttgatg atttcaaatg
    15541 ctttttttcc gccttcttca gcgtttttta cacgaatgat ttccatagtg attgctcctt
    15601 tatattttgg tatagcccaa ttatagcatt ctttttttct ttttacaata attagtatag
    15661 accaaattgc atttttttca ttttcttgac aataaatagg aatccgtgtt aggtttcata
    15721 agatggttca atcaaacaat ccatgttgca tgaaaaccaa gcaagtgatt cactgtggac
    15781 gctttatgtc tgcagttttt ttatgaaaaa ggagttatta tgagtatact gacattaaac
    15841 aacattaccc agcaatttgg cgacaaaatc ctctatgaag gtgtcgatct gcaattaaat
    15901 gctggcgaac atcttgggct gattggacaa aacggagccg gtaaaagtac attgattaaa
    15961 atcatcactg gtgagatctt accagatgac ggacagatcc attggcaaaa aaatattcac
    16021 aaaggttacc tcgatcaata tgtggaagtc gatgaaacat taacaatcga agaatttcta
    16081 aaaactgcgt acgcaaaaga atacgaaaag gaagaaaaaa ttgcttttct ttaccaaaca
    16141 tatagcgaga ccttcgatga ttccttgctg gaaaaagctg ggaaactcca aacggaatta
    16201 gaccaaggag tgttttatca aatcgacacc ttagttgctg agatgagcag cgggttgggg
    16261 atcgatgtct taggtttaga ttcactgttg aaaaatctaa gtggtggaca acgttcaaag
    16321 gtgattttag caaaactact tttagaaaac ccagatgttc tgatcttaga tgaaccaacc
    16381 aatcacttag atgatcagca tatccaatgg ctgacgcagt ttcttcagga ttttgacggt
    16441 gcgtatatcg tgatttccca tgaccaagaa tttcttgatc agatcaccac ccatatcgca
    16501 gacatcgagt ttggaaagat tactaagtat actggccatc taaaacaagc attgaaacaa
    16561 aaggaacaaa atcgcgaaag ctatttgcgc caatatcatg ctcaacaaaa acaaatcgaa
    16621 aagaccgaag cttatattcg aaaatacaaa gccggttccc gatcaacgat ggcaaaaagc
    16681 cgacaaaaac aattagataa aatcgaacgg ctgacgcctc cagcttcagc aagtaaagct
    16741 gtctttgatt ttccattctc gcctattgtc acgacattag cagtggaaac aactgattta
    16801 gtcatcggat atgatcgacc gctacttgca cccatcaatt tgaccatccg ctttggtgaa
    16861 aatattgcca ttcgaggctt caacgggatt ggaaaatcta ctttattaaa aacactgatc
    16921 ggtgagattg aaaaaataag tggcgatttc cacttcccag ataacacaaa aatcaattac
    16981 ttttcacaag atctgtactg ggaaaatcca ttagaaacac cactacaata tttaagcagt
    17041 caatttccaa aagcgactat caaagaatta cgtcgccaac tagctaaagc agggttagtt
    17101 aatcaattag caagtgaacc tctcactaca ttaagtggtg gggaacaaac taaggtgaaa
    17161 cttgcacagt tgacaatgaa ccaaggtaat ttactgattc tcgatgaacc gacgaaccat
    17221 atcgatcaag aaacgaaaga aagcttacaa gacgggatac agaaatttcc aggaacagta
    17281 atcatcgtct ctcatgaaca agaattttat caagatcttg tgcaacgagt cattgagatt
    17341 gaaccaaaat aaacgtttga ttgaatagtt gaaaaaaagg agctgtgaca aaagttttgt
    17401 cacaactcct tcttctgaat aaatggtggc caattagctg accttcggta ttagctcaaa
    17461 aaacggcaaa atatgaggag ctatttttat ttggctattc ttattacttg atgcagaaca
    17521 gcttttcaca acctcttttc gtctattttt tattttcgag atttctcttt cctttaaaaa
    17581 caactgtttg aaagaagaac gcacatcctt gaaaaacaag cccgctaacc gttacaccta
    17641 accagccata ctgctgccac attgtcgtcc ctagcaatga accagccgca cctccaataa
    17701 agtacataaa cataaataca gtattatttc ggttgcttgc tgcttcacct agcccttgta
    17761 ctcgtgtttg attggcgact tgaccaaatt gattcccgac gtctaataaa ataatagcta
    17821 aaagcaaaat ggccaaatgg cttccgccaa ccaataaagc aataaagctt attgtttgca
    17881 taacaagtcc catatacacg atttttcttt cagaataaga atctgccagt cgtcccacga
    17941 taggtgcagc aactgctcca gacaaaccaa aaatcgctaa tatccctgct tcgaatgttc
    18001 cccaattgta ggcgggactg ctgatataaa aaatcaacgt agaccaaaag atcgaaaatg
    18061 ttccaaacat gaaaaatcca ctgactgccg cttctcttaa tagacgttga gaagcaacta
    18121 aatgcggcaa acttttaagt gaatcaaggt atgaaacagc tgtactactt tgctggacag
    18181 aacgcggcat tttccagtaa agcagaagtg taagaattcc gactgcacat aaagcaaata
    18241 aatagatcat ccgccaagaa aaaagactgg caacaatacc agataccgtc cgagaaagca
    18301 aaatccccac gagtaaaccg ctaagcaatg tacccatgac cttccctctt gcttttggtc
    18361 ccgctagtac cgcaccgtaa ggaatgatga tttgcggcac gatcgaaagt aatcctatta
    18421 aaaaagaagc gcatgcaaat aacgtaaacg aaggcgcaaa aaaagcggcc aacagagaga
    18481 cggcagataa cgctgccatt cgaatgatca attttggacg atccaccacg tcccctaaag
    18541 gaactaaaaa cagtaaccct aacgcatagc ctaattgggt caacatcgtc aatatcccga
    18601 ctgcacttgt gctaacagat aaactagctg ctacttttgt tccaatcggc tggatgtaat
    18661 acatattggc aacaacaacg ccacaagtga ttgataatag taaaataagc gaagttgtta
    18721 aaggtttttt ttcattactc ttcattgatt tcccccattt cctatcttag tttacaactt
    18781 atggaaaaga ttggaaatag aaaaatacaa ctattcgcca cacttattat ttgtgctttt
    18841 gttttccttg ttttttcttg cgatctttat aaatgaagac aactaaccct actagcacag
    18901 taacacttgc aaccaacgtg actgcagttt tgttatatcc gtgaaacaga gcttctccac
    18961 cacacgcata caacacagac gaagggatga ctccaatcac gatcgacaat atgatttgct
    19021 gtagaggcaa tttcatcgca tctgcagcaa aattgaccat agaagatgga ataaccggta
    19081 tcatatagcc taacattaca cctatttttg gatgtttcat ttgtcggatt gcttgcaccc
    19141 aatgattcgt ttcggttttc ttatctaaaa attttaagcg atgcataagg aaaattgcag
    19201 tgatgttacc aagagcatta cccaaaatgt tgatgatcgc acctgtcagt ggtccaaagc
    19261 tcaaaccagc aatgactcca actactgaag taggaatacc tggaacagca cacatcaccg
    19321 ctgtcaatac gatcaagagt atcgatgcaa aagtcccatg cgaacgaata tgtgcgagta
    19381 gctctgcctt actcgctctc ggatttaaaa ataattggat gtctgtctga tattctaaat
    19441 acaaccgata gcccactacc agtatcagaa tgattcccaa cgagatcaat atcaatcgga
    19501 ttattttctt tttcatattt cctcctgaaa aaaatctata ttcattaaat aatagtttaa
    19561 cataaaaaca aaaaagcacc gaaatccaac ataggatctc agtgctttta aatagtaaaa
    19621 attatttaac agttacagat gcgccaactt cttcaagttt agctttcaat tcttcagctt
    19681 cttctttaga tacgccttct ttaactggtg caggagcgcc atcaactaca gctttagctt
    19741 ctttcaagcc taaaccagtt gcttcacgaa ctgctttgat aactttaact ttttggtctc
    19801 cagctgcagt taattctaca gtgaattctg tttgttcttc agcagcagca gcgccaccag
    19861 cagcagctac agctacagga gcagcagcag atacgccaaa ttcttcttca atagctttaa
    19921 ctaggtcgtt aagttctagg atagttgctt cttttaactc agcaacgatg ttttcaatgt
    19981 tcaatgccat tataatttcc tccgtttaat tgttcttttt tatttaatgc tttatgcggc
    20041 aattaagctg catctccatc ttctttgtct gccactgctt tgacagcgta agccacgttg
    20101 cggactggcg cttgtagtac agatagcaac atagatagaa gtccttcgcg atttggcaat
    20161 tttgccaatg ctgtgatttc ttccaatgaa gagactttac cttcgatgat accgcctttg
    20221 atttctaatg cttttgcttc tttagcaaat tcgtccatga ttttcgctgg tgcgactaca
    20281 tcttcgttac tgaatgcaac agctgtaggt ccagtgaata cttcgtccat gccttcaagg
    20341 ccagcttttt tagctgcacg tgaaagaata gaatttttga taactttcat ttctacgtta
    20401 gcatcacgaa gttgtttacg taaacgtgtt acttcatcaa ctgttaatcc gcggtagtca
    20461 acgactacta ctgatgcagc tgctgtaaat ttttcgctta cttcatcaac gattgctgct
    20521 ttttttgcga taattgcttc actcattttt gagtttcacc tcctgaattt tgatggaaga
    20581 gttttttgca ctgaaaaggc aagtcacttt tatagttact ttgctcaacc agtattattc
    20641 atcacctatt cacttcgttc atagtgattc tactcgcttt gctcgaaagg aaactccttt
    20701 cgctttgctc aaccagtatt gttcatcatc tcttcacttc gttcatagtg attcacaata
    20761 aaaaactcta tgccaccgca gacatagagg gactattgaa tatattcaat aaacgtcctc
    20821 ggtaggaaat taaggcttga tgccacctac tgtcttcggt acaagttaat tattaacctc
    20881 aactagctta gcatgctaaa ctagttgagt caagaattat ttttcagttg gatcaatcaa
    20941 cattctaata tttaattaga aagaagcttg gtcaacatgg ataccaggtc caaatgttgt
    21001 tgtgatcgtt acatttttca tgtattgacc tttagctgct gatggtttag cttttagcaa
    21061 tacatcatgg attgctgcaa agttttcaac taatttttca tcgtcaaatg aaacttttcc
    21121 gattggtaca tggatatttc cagctttgtc cacacggtaa gttactttac cagctttcac
    21181 ttcgtttact gcttttgtta cgtccattgt aactgtacca gttttagggt ttggcattaa
    21241 gccttttggt cctaatactc gtcctagacg acctacagta gccatcatat caggtgttgc
    21301 aacaactaca tcaaagtcaa accatccgtt ttggattttt tgaaccatgt cgtcgtcacc
    21361 tacgaaatct gcaccagctg cttcagcttc ttttgctttg tctcctttag caaaaactaa
    21421 aacagtttgt gttttaccag ttccgtttgg taatacaaca gcaccacgga tttgttggtc
    21481 tgctttttta gggtctacgt tcaaacggta tgcaacttca actgttgcat caaattttgc
    21541 aatgtttgtt tcttttgcta aagctactgc ttcggcaaca ggataaactt tacttgaatc
    21601 aacttttttt aatgcatctt gcattttttt gctcttttta gccattttgg ttcctccttg
    21661 attgtggtta taacggttgt acctcccacg tgttctatat ctttcaatat agagccaact
    21721 gagaagctgc tgtcttaggg tgtatagatt attctacagt gatccccatg cttcgtgcag
    21781 ttccttcaac catgcgcatt gctgcttcaa cgttagctgc gtttaggtct tccattttta
    21841 attcagcgat ttctcttact tgatcgctag aaactttcgc aactttattt ttgtttggtt
    21901 cgcctgaacc tttttcgatt ttagctgctt ttttcaatag aacagcagct ggcggtgttt
    21961 ttgtaacgaa tgtgaatgaa cggtcttcgt atacagaaat aacaaccgga atgatcaaac
    22021 ctgcttgatc agctgtacga gcgttgaatt cttttgtgaa tcccatgata ttgatacccg
    22081 cttgacctag tgcaggacct actggtggag ctggagttgc tttacctgca ggaatttgca
    22141 atttaacgat tttttctact ttctttgcca cgagacatac ctccttaagt ccgtgatgtg
    22201 gttaattgga gatgcatatt tctcctccca cgttttatct gatctgaaaa aatccagaca
    22261 aataatgtgc aaaacaatgc acactggtac agtatagcaa atttatttgt tttttcaaga
    22321 tttttttctg ctatttttac attttgcttg aataaacgct aaatttattc taaaaaaaca
    22381 acaatttttc gttctcattt cttcttttat gttacacttg cgctagcatg gttcaacaga
    22441 tgaaagcaaa caataacacg gttttttaga cccttgtaca aagattccta cataacaaaa
    22501 tgaggtggag aaaatgaaag atttaaggta tagaagtgtc tttgatatta ttggacctgt
    22561 gatgattggt ccaagtagct ctcacactgc cggagctgct agaatcggga aaatcatacg
    22621 tagtatattt ggcgaacagc ctgattctgt agatatttat ctatatgaat catttgcaaa
    22681 aacctatcgg gggcacggaa cagatattgc attagttggc ggcctgttag ggatggaacc
    22741 cgatgatgaa cgtttagctg attctttgaa attggcatat gaagccggta tggaagtctt
    22801 gtttgttcct aagagtgaaa aagccgaaca tccaaactct gtaaagatgc ttctaaaaaa
    22861 aggaacacga aagctttcag tcactggcat ttcgatcggc ggtggcaata tccagatttc
    22921 tgaattgaat ggattcaaaa tttctttaag catgggtact cctaccttga tcgttgtcca
    22981 ccaagatgtt ccaggtatga ttgctaaagt gactagtata ctttctgaga ccaatacgaa
    23041 tatcggcact atgacagtga cacgggaatc aaaaggtgaa aatgccatta tgataattga
    23101 agtagatgat cctcaagtag aagaaacggt aaaaagactc aaacagctgc caaatatcga
    23161 cagcgtaaat tatttcgatt aaggagctaa aaaaatgttt ttatcgattg aagaattagt
    23221 agatcaagca aaagcttatc caagtgtagc agaattgatg attgcagttg aaatggaaat
    23281 gagtggccgt tcccgtagtc acatcatcga gatcatggaa aaaaacctct cagtaatgga
    23341 acaatcaatc aacgagggaa aagaaggtgt tacctccgtc acaggaatta ccggtggtga
    23401 tgcaaaaaaa ttagatgatt atttaaatga ggcagacttt ttaagtggag aaacaatctt
    23461 aaaagctgta cgcaatgctg tagctgttaa tgaagtcaat gcaaaaatgg ggttgatttg
    23521 tgctacaccg actgccggaa gcgcgggtgt tgttcctggt gtgctgatgg ctgtccgaga
    23581 tcggcttgat ttgacacatg aacagcaatt ggaattctta tttaccgctg gcgctttcgg
    23641 gttagtcatc gcaaacaacg cttctatcag tggtgcagaa gggggatgtc aagcagaagt
    23701 tggttctgca agtgcaatgg cagcagcagc tttggtttgt gcaaaaaatg ggactcctca
    23761 tcaagcagca caagcagtag ccattacatt gaaaaatatg atgggactga tttgcgatcc
    23821 cgttgcaggc ttagttgaag tcccgtgtgt caaacgtaat gctttaggct cttctcaagc
    23881 ttttatctct gctgatatgg cattagcggg tatcgaaagt gtcattccgc cggatgaagt
    23941 cgttgctgca atgtaccaag tcggacgtca aatgccgtcg attttcaaag aaacagcaga
    24001 aggtggctta gctgttacgc caactgctaa acgacttgcg gaagagattt taaataagag
    24061 ggcgtgataa agctccttaa tttcgagtat taagagatta gttatcttca taacaaaaaa
    24121 cgaagagagg actaattgcc cttctcttcg ttttttatta atccatcaat ttcgtatttt
    24181 ttacttcatc gatcgttttt gttcctgcaa gttgcatcgt gatcgacaat tctttgttca
    24241 ggtgttcaaa aacagatgtt actccttctg ccccacctag attcaaccca taaatcactg
    24301 gtcggccaat cgcaactaag tcagcaccac ttgccagagc tttgaagaca tgctctcctc
    24361 gacggacacc agagtcgaaa atcactggta cacgtttgtt gactacttcg gcaatttttg
    24421 gcaatacttc aaaggacgca ggacctccat ctagttgacg tccaccatga ttggataccc
    24481 agataccatc tgcccctgca gagatagcga ttactgcatc ttctggcgat tgtattcctt
    24541 ttacgataac tggaagatgg gtgatttctt tgattgtttt gatgtcatct ggtgtcaagc
    24601 cttgttttgc agctgcataa atttctgcga ttccttttcc ttcgccatta ccacttgcac
    24661 tttgttcgct atacgcagct aagttaggca ttggtaatgg aaattggaat tgattgatga
    24721 cgtcttcttc acggtaacca ccgagcgttg aatctgccgt taaaatgata gctttagccc
    24781 ctgcttctac tgctttgttc aaaatgaact cattgaatct gtcgtcttta ctcatatata
    24841 attggaaaaa ctgcggtgcg cctggcgctg catcggctgc gtctttgatt gtcgtatttg
    24901 cataagtgct gatggaaaag attgatccag cagcagctac tcctttagct gtatcagctt
    24961 cccctttttc atgtgccaat ccttgggccg ctgatggcgc ttggataatc ggtgtatcta
    25021 attcaattcc gaaaacagat gtatgcaaat cggctgagtc gattccgcgt aatatccgtg
    25081 gcatgatttt ctttgtatta aaagaagtcg tattttcttt catcgtccac tcatcttcag
    25141 aaccaccgcg aatatagcca aaagctcctg cttccatgcg ctcttttaca cgaccttcta
    25201 gttaggcaat gtttacaata tcgattggat gttcttcatt acttgtatga taagtcatag
    25261 tagatccttc tttcatcttc ttgattacat ataaatcata ctaaacttac aaaaagtaag
    25321 caaacattta ctctcagata cttttatcgc tattctttcc aaataaatcg acttacaagc
    25381 tgatctactt cttggttttc atgaagcata ctatgagagg cattttcacc tgtcactact
    25441 ttttcctcta tttcatttcc gcgttgcttc aacaacgcat agaccgctaa tgcactgttt
    25501 aacggtactg ttccatcact caagtctgtt tcatctagtt gaccagcaat caagaaaaat
    25561 cgagtggtta taggaatatt accgatcatt tgctggtaat cttgatagcg gttagaaacg
    25621 acagcaggcc cctttgctaa ctcatctgtc agagactgcg cactatcatc gacaaaatca
    25681 ttaaaaggcg cgccaattgc tgttaatgtc tgaacttgtg gcaatgacgt atcctgtcca
    25741 tacgttccta aatagcgtag tgcactaact cctcccatgg aatgccccac tatgttgact
    25801 ttatctatgc cgtagttttc tttcagatac aaaagacaag ttttgatcca ttctgcttga
    25861 ttccactcgt tatttttatt atcttcaaac aatacttgga ccatcggatt gtctgctttt
    25921 ccagacaaat ctcctgttgc ttgtatttgt ccatctgttc ctactgttaa aaccaattct
    25981 tttactgctt gctgattttt ttccaaacgc cgcaacatcc ctttaaagga attttctgtc
    26041 ccgctgtagc cgtggaaaaa aagtgttgga atagtggttt tttctatcac tttactggat
    26101 tctgccgttt tttctttatc agaaacttca gtagtattta tcttcgtcgt ctggcaacca
    26161 cttaacgcaa acaaactgat aaggactaaa gctgctgcag ttgcacgttt gcataaattt
    26221 ttgattggtt ctgtcataca cctcttcctt tccatataga aaaaagaaag acttgaaagg
    26281 ccaatcatat gatcgacatt tcaagtctct ttattaatcg attttatcta cttgatcgaa
    26341 atcaagttct gtactggttt cacgtccaaa catatcgatg ttgactttca atttttcttt
    26401 ttctccgtca acttctgtta cttgtccttc taatccagca aatgcgcctt cgatgatttt
    26461 cacgacttcg ccaacttcta cttctagatc attttgtcgc aggctcatac cgatagaacg
    26521 caagatatga ttgacttctt ctggaagtaa tggtgctggt ttgcttccgg ctccgtgtga
    26581 accaacgaat cctgtcacac caggtgtgtt gcggacaaca taccatgaag cgtctgtcat
    26641 gatcatttca acaagtacgt atcctgggaa tgttttatga acgatttctt tttctttccc
    26701 atttttgact tcagtttctt tttcttcagg aacaacgaca cggaaaataa agtcggccat
    26761 tttcatactt tgcgcacgag attcgatatt tgcttttact ttgttttcat atcctgaata
    26821 tgtatgcaag acataccagt ttttttcaaa agattccatt gaaggctcct catttcattg
    26881 taaaataaaa aaacctcagt acacccgaag tttttctttc gcttcattat agcactaaaa
    26941 gtacgttgtg gctagcgagt tattttaaaa tccagccaaa taagctttgg atggctgtat
    27001 ccattacgta aaacatcaat ccgaaaataa tagaagtttc gatgactact aacgtatctt
    27061 tacggagttg ttttctgctt ggccaagtta cttgtttgat ttcatctttt acgcttttta
    27121 gaaatttcat gagacaacct cccttgctac tgtttatttc gtttctttat gtaatgtata
    27181 ttgattacaa tatttacaaa acttattgat ttccaaacgt tcgccgcttt ttccttcact
    27241 gactgatttt gtgtagttgc gagacccaca aacggaacaa gcgagtgctg cttttttctt
    27301 tgccatttac ttctgccccc ttgctgagaa tcagacttat acttcataac agtatcacgt
    27361 aactttttac ttgtcaattt gtgaaataaa ctatctattg ttttggataa aattatgata
    27421 tgattaattt gaaattaagc taaaatgaga caattggagg tatagcgatg ctattaaaaa
    27481 cacttgtata taagaaaaaa gatctgacaa ccgttacaga gactagtaca ttagaagaag
    27541 cattaaaaat tttagaaact tccggatacc gttgtgtgcc gatcttagat tcttcaggta
    27601 atattttccg tggaaacatt tacaaaatgc atatctatcg tcataaagca aatggcggtg
    27661 atatgacgct tcctgtcact catcttttga aaaacgctac aaagttcatt tacctggata
    27721 cttctttctt caaagtattc tttacgatca aagagcttcc ttatattgct gtccttgaca
    27781 gtgaaaatca tttttatggt attttgacac atagtacgtt gcttaatatg ctctctcaat
    27841 cttggagcgt agaacaagga agctatgtac tgacaattgc ttccacagga aagcaaggag
    27901 atttggctgc tattgcaaaa atcatttcaa aacacagcag cattgccagc tgtattactc
    27961 ttgatatcga aaaagacgaa tacattcgac gtacattgat cacgctaccg gctaacactg
    28021 aagcgaaagt atgtgaaacg atcgtcaacc acttagagaa aaagaattat aaagtggtag
    28081 aaattgaaaa tttgcaaaac caagaagcat aatcaaaaag cgaaaaaaga aaacagctaa
    28141 tctgctttct tttttcgctt tttataatta gacacgcttt tcaaaatcaa gcgcgcgatc
    28201 taaataatac atcaatggtg ctgaaacagc catgacgata aactcactta atgcaagcgt
    28261 gccataggtt gtccaaaatg cttggccacc tgttggaact aacatataag caatcagtcc
    28321 catgctgatg gtaaagaaga tcgtgttcaa tactaaacgc atctttacat ttttcacttt
    28381 gttttgcaat aaagctgtca ggcctaaaga aagcaatgtt tggccgccac caaaaaccac
    28441 atccatgata ccaaaaccaa agaaggcatt atagacgatg accccgccta aaactcccca
    28501 aagcaattta cggttaaaga caactaaatg attcaaactt tctgaaatac gaaactggat
    28561 cggccctgag gatacaggcg caactaaaat agttaacgct agataaagag ccatgatgat
    28621 gccatttaca acgattacgg ataaccgttg tctgcttttc gttttttgca ttttctctcc
    28681 tcttttctcc tagttttttt aacgcgggat ggttgatgaa ccgcggttat gtgaaacaaa
    28741 caataattat aacagacttt tcatttattt caatccgtct tatctgcttt taaaaaagca
    28801 aaagcttaaa caatgtaaac gtttgctaaa aatcgtggga tatggtataa agtaagtgta
    28861 aggggtcata agaattccca gtaaagcaga cgaagtagga tctgcaagaa cagaattgtt
    28921 gtgaaactta aataaataaa aaaggatgtg ggaatttgag caacctctta ctagagaacg
    28981 gtctagcaag cagctgaaga tctaagtgga catgccgaac cttggaactt ctccttacgt
    29041 aagtaatgga tcatgcgctt gctaggcttt tttgtgtttt tcggtcttcg gacctttttc
    29101 attttcctag acttctgcta taataaccaa gaattaattt tgtaacagat ttttttgaaa
    29161 aaacaagttt ctttcattca aaaaatagaa aaagcttgct aattattggt taaccagcaa
    29221 acaggagtat taaaatatct tgttttattg aatagcagga ggaaatcatg aaattccatt
    29281 atcatatatt gatcaatcca tcggcaggca gcggaaatgg ctataaagta gcagaaagaa
    29341 ttttaccggt attaaaaaat aagcatattg actatacact tcattatagt gaatacaaag
    29401 gacacgatgc tgagattgct gaaactttgg caaaagaaac attgattcct tggatagaag
    29461 aagaacagaa aacaatcgat actttccccc ttttgatcat cgtaggaggt gacggcacct
    29521 tacatcaggt acttgatact ttctatcaga tggaagtaga attcccagtt gcttatattc
    29581 cagcaggttc tggagatgat tttgctcgag gggcaggcct tccaaaaaat cccaaaaaag
    29641 gacttcagct gattttagca gctcaatcac cagaaaaagt ccacataatg gcttatgaag
    29701 aaaaaataag tgaaaaaaaa ggaatagctg ttaataattt cggtatagga ttagatgcag
    29761 ctatcgtcca tgcgacaaat cattcttcca caaaaaagcg attgaacaaa tacaacttag
    29821 gatcattttc gtatttattc tctattctcc gtgctttatt cactcaaaaa ggatttccaa
    29881 ttctggtaga tgcaggagga aaacaattaa gtttttcaaa cgcattttta tgtacagcaa
    29941 cgaagcatcc tttttttggc ggaggaatcg cgattgttcc aactgctgat gcgtctaaac
    30001 cagtcattga ttttgtcgtg gtcgaacgaa tcaatttctt taaaatcttg tggctcattc
    30061 ttttacttat acagaaaaaa caattgaaat cgaaatattt ccatcactac acaacaaaca
    30121 gattacgtat cgtttcaaca acaccgcaac atggtcaaga agatggagaa gaaatggaaa
    30181 aacgaccttt tgatatcaca ataaccacaa ctaaacagtt gttttggtgt taataaaaga
    30241 ggttttgtca gaagctacat ctggaacacc gtttattcgg aaaaaaggac tgtgacacga
    30301 cttttgtcac agtccttttt ttactatgca gttaattctt tgcccaattg ggttgccatc
    30361 gatagtttct ttctttaccc aaaataaatt ggatcattga aaacaccgca gcaaaagcaa
    30421 ctaagacaca accattgaac caaaaagatt ctgcaaatgt ttgattgtaa ctgagtgttt
    30481 cactgatcaa gttattattt tgaacaaacc aatatgttgg tgtgaatgcc gctattttgc
    30541 taaccatttc tggcaagtat tctgctggta caaacacccc accgataaag caactgcctg
    30601 tgataaaaat atctttgatc ccaccgctgg tttctgagtt ttttaccaat gaagtgatac
    30661 aagccgaaaa gctaatgatc ggcaataaaa acaagaaagt atttaaaata tgataccata
    30721 ccgttatatc ccacgaggtg tggatcacta acagaaccat tagaagtaag aagccactga
    30781 acaacagcag cgagtaacta atcaaactaa aggttacttt gcgattcaag tttcgtttgg
    30841 taatgggcgc actctggttt cgtttactga tctctttgcg attaaatgct agattgatga
    30901 cgctgaaacc gctaaaaatg gatagaaaca acccataaga caagaaatta aaggttgtgc
    30961 ccgtcaacag ctttttcatc cgctgactat atcccgcact tagctcaact tctccagtaa
    31021 ccgagagtgc catttgtgtc tgttccatag ctgcatcgat tgttttatct tcttcttgaa
    31081 acagaatcag tgtttgcaaa aaggtgttca cttgctgtgt aacaactgtt ttagaaaagg
    31141 catctgggcg actctgtatc ttgatcgtcg gcatctttcc tacttcgact tgttcggtaa
    31201 agtctgcagg cagatgtaag ataatgttta cacgattaaa ataaagagca tcatcgattt
    31261 ctttttgtga ggttccatcc aatgtaacga ctgtttcctt gtcattcaaa tatttacgaa
    31321 ggccatcgac tacaggcgac tcttcttcac taatcaaggc gattttcgca tcttgcagag
    31381 aggtattttc aacatcaata ttttgcgcaa aaaagaatgt ggcaacaaca gtgataatta
    31441 cacctatcag taaattcgat ttatttgctt taagcaatgt ccaaatcgtt ttataaagcg
    31501 tcataacgca cccctctttc catccaagca tttcctaacg caaaaaggaa aataaatccg
    31561 atcaaccata gaagattggt ataaaaacta gccaaatcag tgtagaaata aagttt
//
LOCUS       DAYYDS010000027        30382 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000027 DAYYDS010000000
VERSION     DAYYDS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.027"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..524
                     /locus_tag="ACHA02_001191"
     CDS             156..524
                     /locus_tag="ACHA02_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8669551.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            704..1645
                     /locus_tag="ACHA02_001192"
     CDS             704..1645
                     /locus_tag="ACHA02_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8669552.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1714..2445
                     /locus_tag="ACHA02_001193"
     CDS             1714..2445
                     /locus_tag="ACHA02_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8669553.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2589..4769
                     /locus_tag="ACHA02_001194"
     CDS             2589..4769
                     /locus_tag="ACHA02_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8669554.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4789..5271
                     /locus_tag="ACHA02_001195"
     CDS             4789..5271
                     /locus_tag="ACHA02_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8669555.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5287..6048
                     /locus_tag="ACHA02_001196"
     CDS             5287..6048
                     /locus_tag="ACHA02_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8669556.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6179..7513
                     /gene="murC"
                     /locus_tag="ACHA02_001197"
     CDS             6179..7513
                     /gene="murC"
                     /locus_tag="ACHA02_001197"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8669557.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7820..8305
                     /locus_tag="ACHA02_001198"
     CDS             7820..8305
                     /locus_tag="ACHA02_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8669558.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8298..8981
                     /locus_tag="ACHA02_001199"
     CDS             8298..8981
                     /locus_tag="ACHA02_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8669559.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9107..11752
                     /gene="polA"
                     /locus_tag="ACHA02_001200"
     CDS             9107..11752
                     /gene="polA"
                     /locus_tag="ACHA02_001200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8669560.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11797..12633
                     /gene="mutM"
                     /locus_tag="ACHA02_001201"
     CDS             11797..12633
                     /gene="mutM"
                     /locus_tag="ACHA02_001201"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8669561.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12597..13226
                     /gene="coaE"
                     /locus_tag="ACHA02_001202"
     CDS             12597..13226
                     /gene="coaE"
                     /locus_tag="ACHA02_001202"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8669562.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13278..13610
                     /locus_tag="ACHA02_001203"
     CDS             13278..13610
                     /locus_tag="ACHA02_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8669563.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13922..14413
                     /gene="nrdR"
                     /locus_tag="ACHA02_001204"
     CDS             13922..14413
                     /gene="nrdR"
                     /locus_tag="ACHA02_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8669564.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14436..15809
                     /locus_tag="ACHA02_001205"
     CDS             14436..15809
                     /locus_tag="ACHA02_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8669565.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15809..16735
                     /gene="dnaI"
                     /locus_tag="ACHA02_001206"
     CDS             15809..16735
                     /gene="dnaI"
                     /locus_tag="ACHA02_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8669566.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16864..17304
                     /locus_tag="ACHA02_001207"
     CDS             16864..17304
                     /locus_tag="ACHA02_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8669567.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17448..18149
                     /locus_tag="ACHA02_001208"
     CDS             17448..18149
                     /locus_tag="ACHA02_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8669568.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18146..19267
                     /locus_tag="ACHA02_001209"
     CDS             18146..19267
                     /locus_tag="ACHA02_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8669569.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19282..20514
                     /gene="pepT"
                     /locus_tag="ACHA02_001210"
     CDS             19282..20514
                     /gene="pepT"
                     /locus_tag="ACHA02_001210"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8669570.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20646..21554
                     /locus_tag="ACHA02_001211"
     CDS             20646..21554
                     /locus_tag="ACHA02_001211"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8669571.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21589..21861
                     /locus_tag="ACHA02_001212"
     CDS             21589..21861
                     /locus_tag="ACHA02_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8669572.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21872..22918
                     /locus_tag="ACHA02_001213"
     CDS             21872..22918
                     /locus_tag="ACHA02_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8669573.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23169..25778
                     /gene="clpB"
                     /locus_tag="ACHA02_001214"
     CDS             23169..25778
                     /gene="clpB"
                     /locus_tag="ACHA02_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8669574.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25929..26615)
                     /locus_tag="ACHA02_001215"
     CDS             complement(25929..26615)
                     /locus_tag="ACHA02_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669575.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26593..27087)
                     /locus_tag="ACHA02_001216"
     CDS             complement(26593..27087)
                     /locus_tag="ACHA02_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8669576.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27107..28327)
                     /locus_tag="ACHA02_001217"
     CDS             complement(27107..28327)
                     /locus_tag="ACHA02_001217"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8669577.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28474..30309
                     /gene="lepA"
                     /locus_tag="ACHA02_001218"
     CDS             28474..30309
                     /gene="lepA"
                     /locus_tag="ACHA02_001218"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8669578.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        10191 a         5409 c         6552 g         8230 t
ORIGIN      
        1 agcgcaaata acaaaaacaa ccaacttatt tattattaaa gaaaagcgta tttaagtagg
       61 acgattctgt tgagagcttt cgtagatttt cccattttcc tatggtaaac tgacaaagtt
      121 gagaatgtta gaacagatcg tttaggagga aacagatgtt tacgaatgaa ataaaaatca
      181 tgctttatgt ggacgatgta gaaaaaaatg caacgttttg gagagcagtt ggctttgcgg
      241 aaaaagatcg tcaagaaatg gatgggacgc ttataattga agtaggagtt gaggacagtc
      301 aaacctcatt tctgttatat gatcgagcat ttgttgagtc ccattcacct ggcacagcta
      361 ctagtgcccc atctttgatg ttttacagta aagatatctt tggactatat aaaaaaatgc
      421 agacgcaatc agtcacatta ggtgatatgg tacagcttgg agaagaatat gtctttaact
      481 ttgctgatcc cgatggcaac tactttgcgg ttacaggtaa ataatagaaa taaagacttt
      541 gtttttctgt gcgaaacagg aaagcgaagt ctttttaatt caaataataa tattatgtaa
      601 acaaaaaact ttccatgcgt aattatacac aagcaaataa atcgtcaaat gcgaacgcct
      661 tctttatact gtttaggaac gaaaagaagg aaggaagaaa tacatggatt atcaagtact
      721 gctttattac aattatacaa aaatcgaaga accagaggaa tttgccaaaa atcatttagc
      781 cttttgcaaa tctttggatc ttaaaggtcg tattctagta gctgaagaag gaataaatgg
      841 tactgtgtct ggaaccgttg aaaacacgac tgcctatatg aaagcaatga aagctgatcc
      901 tcgatttgct gatacgatgt tcaagatcga tccaagcgaa ggacatgcct ttaagaagat
      961 gtttgtaaga taccgtccag agcttgtaag tttaaagcta gaagaggaca ttgatccaaa
     1021 ccaagtgaca ggtgcctatc tttcaccgca agaatttaaa gaagctattt tagatgaaca
     1081 gacagtggtg attgatgcac ggaatgatta tgaatatgac ttgggacatt ttcgaggggc
     1141 tgtacgccca gatatacgca gttttcgaga gcttccagac tggatccgcg agcacaaaga
     1201 agaatttatg gaaaaacggg tagtgaccta ctgtactggt ggcattcgtt gcgagaagtt
     1261 ttctggttgg cttgtccgtg aaggattcaa agatgtcgga cagctgcatg gcggtattgc
     1321 tacttatggt aaggatcctg aagtccaagg agaattatgg gacggcaaga tgtatgtgtt
     1381 tgatgaacgg atcgctgtcg atatcaacca agtagataaa caaatcgtcg gtcgcgactg
     1441 gtttgacgga acaccatgtg aacgatatat caattgtagc aatcctgagt gcaatcgtca
     1501 gattcttgct tccattgaaa atgaagaaaa acatttagga gcatgtagca tagagtgtgc
     1561 gaaacatccg cgcaatcgct atgttcaaga ccatggaatc tctccagaag aagcagaact
     1621 gcggtttaca gctttaatta aatagaaaac aatagttttg attgtcgacc atttccaata
     1681 tgcgctatac tagcgttgag gatggtgatc cacatgaaat cattgattta tgctattggg
     1741 gatgtccatg gcaaacatga gctttttgaa aaacttattc actattatga tccaaagatc
     1801 catcagcttg ttttattagg ggatctcaat gatcgaggtc ctcgtacaaa agaatgtttt
     1861 ttattaggaa agcaattagt cgaagaaaca ggagccattt acctccgggg aaaccatgaa
     1921 gaatattttt tgcaatttct gcaatcaccg gaggactggt atgcgcctta tatgagaaat
     1981 ggtggaaaag aaacgtttga aagcctgatt cacccagggg catcggagga atactcccca
     2041 actgaaatct cgatgatgat ccaatcgaga tattcagaac tgattaaatt tttagtccag
     2101 cgccccttgt attatgaatg ggaaaattat ctttttgtcc atgccggtgt ggatttgaca
     2161 aagaaagact ggcgtaagac aagtctaaga gactttattt ggataaggga accatttcat
     2221 gaagggaaaa acaatacagg taagacaatt gtatttggac atacgattac accaatgctc
     2281 catggagata tgcagacaac agacctctgg attcaggatc ataaaatcgg aatggacggt
     2341 ggggctgtat tcggagggtc attgcatggg gttgtttttg acaaggaagg tattcttcaa
     2401 gatattgaat atcagaacaa cgaaggacct tggcatggaa tctgactaat ggaaagaatg
     2461 aaggagctgt gactttgttg cggctctttt tatttttata gagaatattg tttctttcag
     2521 tagtttaacc caggataaaa agtatgatag aataaagaag caatttttag cagaaaagag
     2581 gcattttaat gacggaaaat ttgaaccaga cgatcattca gcttgtacaa aaagaacttt
     2641 cagactatcg tcctaagcag ttgactactg tacttaattt gttaaatgaa ggaaacactg
     2701 ttccttttat cgcgcgttac cgaaaagaaa tgactggaag tcttgatgaa gtccagattc
     2761 gtgaaatcga agagcggtat gcctatcttg aaaacttaga aaaacgtaaa actgaagtga
     2821 ttcggctaat cgatgaacaa gggaaactga ctcccgagtt agaagcagaa atcacccaag
     2881 ccgtgaaaat gcaacaagtg gaagatttgt atcgtccata caaacaaaaa cgacggacaa
     2941 aagcaacgat tgctaaagaa aaaggacttg aaccactcgc gaagtggctg atgcagctta
     3001 cagatggaga ggtgcaatct gaagcagaaa aatatattga caaagaaaaa gaagtctctt
     3061 ctgccgaaga agcgttgcac ggtgctcatg agattattgc tgaacaagtc agcgataatg
     3121 caaaatttcg gacttggatt cggtcttata cgtataacaa aggtatgtat gtcagcaatg
     3181 tcaaggatga gcaagcagat gaaaaaggcg tatatgaaat gtattatgac tttgcagaac
     3241 ccgtccataa aatggtttct catcgaatat tagctactaa tcgtggagaa aaagaagata
     3301 ttctgaaagt attctttcaa gtagatgaag cagcaatttt agcttacttg gatcgccaaa
     3361 tagtcaaaaa tcctgcctct ccttcttctt cattcgtcag agaagcctat caggatagtt
     3421 ataaacgctt tattcagcca gcaatcgagc gagaactgcg caatgaattg actgaaaagg
     3481 cagatgagca agcaatcgct attttcggcg aaaacctccg taatcttttg ttgcaaccgc
     3541 cactaaaagg taaagttgtc ctgggatttg atccggctta tcgtactgga tgtaaactag
     3601 cagtcgtaga tgcgacaggg aaagttttag caatcgaagt tatttatcct cataaacctg
     3661 cagcgcaagc aaaaagagaa gctgctggac cagcttttat ccaattaatt aataaatatc
     3721 aagtagatat ggtagctatc ggaaatggta cagccagtcg ggaatctgaa ctttttgtgg
     3781 ctgagcagtt gaaatccgcc gaccataaaa cctattacgc tatcgtcaac gaagcaggcg
     3841 cttctgttta ctccgctagc gaaattgcac gaaaagaatt tccgcatcta caagttgaag
     3901 aaagaagtgc tgtttctatt gcccgtcgcc tgcaagatcc tttagctgaa ttagtcaaaa
     3961 tcgatcctaa agctgtcgga gtaggccagt accaacatga cgtttctcaa aaacgtttgg
     4021 ctgaacaatt ggattttgtt gtggaaacag cggttaacca agtaggtgtg gatgtgaata
     4081 cagcaagccc gcagctcttg cagcatatat caggattgaa taaaacaact gcccaaaaca
     4141 ttgtcattta tcgtgaagaa aacggagaat tcaccgcacg aactcaattg aaaaaagtgc
     4201 cacgcttagg accaaaagca tatgaacaag caatcggatt cttgcgagtc cctggtggga
     4261 aaaacatttt ggacaacaca ggtatccatc ccgaaagtta ttctattgca aaagaaatat
     4321 taacttcagt ccaactttca gaaaaagagc ttggtacaga agaagcagta gaaaaattga
     4381 cacgattatc cgtagagaaa ctggctgaaa gcctatcagt cggagaagaa acgttagccg
     4441 atattttagc tgggctgaca caacctggac gagatatgcg tgatgagatg ccagcgccct
     4501 tattacgtac agatgtattg agtatggaag acttgaaacc agggatggaa ttgactggaa
     4561 cggtccgtaa tgtcattgac ttcggcgcat ttgttgatat cggtgtgaag caagacggac
     4621 ttgtccatat atcgaaactc agcaaaaaat tcgtcaagca tcctacagat gtcgtgtcag
     4681 taggagatat cgtaacagtt tggatagaac aagtcgatac gaaaaaaggc agaatcagtt
     4741 tgacgatgct ttccccttat gaagagtaac aatgaggagt aacaaaaatt gacacaagaa
     4801 gaactgcagc agttagtaga agagatatca ttaaaaagct tccagcttcc tttcaagcac
     4861 caagcaatgt tcaatccacg cttacgtaca actggcgggc gctatcattt aaacgatcat
     4921 catttagatt ttaatgcgaa gctatttgaa gagtcagacg aagagacgat tgcaggaatc
     4981 atcaaacatg aactttgcca ttatcatctt catctagctg gaagagggta tcagcataaa
     5041 gatatggatt tcaagtatct tttgaaaaaa acaggagggt tgcgctatgc accagccttg
     5101 acgaagaaac agataaagat ggagtattat cgatgccaag attgttctgc cacgatctgc
     5161 cgcaagagaa gaatcgatgt acgcaaatat cgttgtggaa gatgtcatgg agaaatcaaa
     5221 tggattgaga caaaaagaga agactttaat gaagcacaaa aaatcagatg aaaggataga
     5281 aggcctttgg aaaaaagtga gcagaggaat ctaaaagact ggaatcagct attaaatttt
     5341 ttagccgcac ctgaagaggg aaattttgag gaactgacgg cacctacact tattttagct
     5401 ggaaactgtc ttcctatctt agctgataaa gcagcacaaa tgtatttaaa tggccaagta
     5461 gatcaattgt ttttggtcgg aggggtgggg catgcgacta gaatccttta tgagaatttt
     5521 gaaaagcaag gatttcattt tgaagatggc atgagtgaaa gtgaaatctg tcgtcagtat
     5581 ctaaaagaag tctatgattt gcccgataag gcttttctga tagaatcgaa gtctacaaat
     5641 tctggagaga acgccacttt ttcactggag attcttcatt ccttaggagc tgtaccagaa
     5701 aaaatcttat tgatgaatga tccgacactg caacgcagga ctcgagcaac ttttgagaaa
     5761 gtatggcaaa atgaacaaac tattttcgcg aatgctgtgc catttgtgcc ggaaatcctt
     5821 cacttttctg aagagatcat ctttacagca aaagaactga atcatcaatg gcctaaggaa
     5881 tatttccatg cgctagtatt aggagaaatg gaacgtctcc atgacgatga gaacggctat
     5941 ggtccgaaag gaaaagattt tattcctcac attgatatat cagaggaagt ttggtcttct
     6001 tacaccagaa ttaaagagtc aataaaaacg gatttttctc gtacttgacc attttagcaa
     6061 gcgaatcctc ctttccaaat aaatttggtt gaaattttca atcgtttata agataatgag
     6121 gagcgtgttg aatgtaatag ctaaaagaat ggacgctaga taagagaaga ggattcaaat
     6181 ggaaaatcaa aataaattgt atcattttgt tggtatcaaa ggttcaggaa tgagctcctt
     6241 ggctcttgtt ttacatgaac aaggtctaaa tgtccaagga tcagacattg aaaaatattt
     6301 ctttacacaa agagatttag aaaaagcaaa tatcacgatt ctgccattta acgcagataa
     6361 tgtaaaacca gggatgacga tcattgcagg aaatgcattt cctgattcac atgaagaaat
     6421 ccagcgagca aaagaattag gtttggaagt gattcgctat cacgacttta ttggtcattt
     6481 tatccaaaat tatacgagta tcgctgtaac aggctctcac ggaaaaacaa gtacgactgg
     6541 actgctctct catgtattga gtggtgtccg tccaacaagt tatctaattg gagatggaac
     6601 aggacatggt gatccgcaag cggaattctt ttcatttgag gcctgtgaat atcgccgtca
     6661 tttccttgcc tattcaccag actatgcaat catgaccaat atcgattttg atcatccgga
     6721 ttattataca agtatcgacg atgtatttac agctttccaa acaatggctg gacaagtgaa
     6781 aaaagctatt tttgcgtatg gtgacgatgc ttatttacgc aaattaaaag caaatgtgcc
     6841 gatttattat tatggtgtca cagaaaacga tgatatccaa gcacgaaaca tcgaacgcac
     6901 aacttctggt tctgcttttg atgtctatca tggggatgaa tttgttgggc acttcactgt
     6961 accagcattc ggtaaacaca atatcttaaa tgctttaggt gttattgcag tagcttattt
     7021 cgaaaaactg gatctgaaag aagttgcaga agaaatgctg actttcccag gagtaaaacg
     7081 tcgattcagt gaaaagatcg tagcagacat gaccgtagtc gatgattatg cgcatcaccc
     7141 tgcagagatc aaagcaacga ttgatggcgc acgccaaaaa tatccagata aagaaataat
     7201 tgcagttttc caaccccata cgtttacgcg tacgattgca ttgatggatg aatttgcaga
     7261 agcacttgat ttagctgata aagtttactt atgcgatatt tttggttcgg ctcgggaaga
     7321 acaaggaaac gttaagattg aagaccttgg agcgaaaatc aaaaaaggtg gagaagtcat
     7381 caaagaaaat aatgtatctc cattgttaga ttatcatgat gcagtagtaa tcttcatggg
     7441 tgctggagat gttcaaaaat ttgaacaagc gtatgaaaaa cttttaagca gcacgacgaa
     7501 aaatgtactg taatacaatt actgcagtgt ccttataaaa atagaaagag ggggagtggc
     7561 aaaaatcttg ccaagccccc ttttttgcaa cttatgataa acggtgttca aaagcttgca
     7621 tctgcggtaa taagcccaat caaatcaaaa acatgagaag ctgttttttc ttggctgttc
     7681 ttattatttg atgcaggaca gctttttcac gacctctgta aactatagga atctgttaaa
     7741 tcaagggagc agaacttgcc ttttacgcta cttttggtac aataatgtat gattaaaaat
     7801 aaactggagg gtttctatct tgaacatagg taagccgaga aaactaggaa aaacaataaa
     7861 tgaaatcgag gaaggcgact cgttatcctt aacagaatct attgaagata accagctttt
     7921 gctgtatttg ggattgacga atgatgcaaa tcctttgtat atccaacatg actttgcaca
     7981 aaaaacagaa tatcaacagc caatcgttcc atcagtgatg ctgatgggga tcatcacaag
     8041 tgcaatttca aagcatcttc ctggaccagg atcacatgtt gtcaattttt ctgtcaattt
     8101 tattgaaccg gttttccatt atgaaacact gacttttcag tttgaggtca taaaagtaga
     8161 taagatgaag gatgtggtga cgatttctgt agaagcggag aatacgaacg aagatcgtgt
     8221 gttggatgct gtcgttatgg tccaaccgcc acaagttcct gaattggaaa tagcaatcga
     8281 aagtgaaggt gaattgaatg aataatccaa gtacatcaat gtcaggtatc aatcgttttt
     8341 atgcaaaagt ttatggattt ttaggtatgg gacttgccct tagtgcagtc acttcttact
     8401 tggctctttg ggtatttcct ttgcaattag caacttttat caataatttt ccactaggct
     8461 ttgtgggtct gtggatcgtt gaactgattt tagtaatggt tctaggcgta aaagcacaaa
     8521 agaatccgag cttagcaatc ggtggattta ttgcctactc tcttttgaat ggcataatat
     8581 tagcggttac tcttgcatac tacgatattg gaacagtaac gcaagctttc ttgacagcta
     8641 ctttgatgtt catggggatg tctgttgtcg gagtaacaac aaaaagagat cttagcgggg
     8701 ttggaagagc aggacaggca gctttaatcg gagtgatcat tgcactagtc ttgaacatct
     8761 tcattttgag aagtagcgta gttgagctgt tcatctccct actgttagtc gtcatctttg
     8821 cagggattac agcttatgat aaccaaagca tccgtaatat ctatgtccag acaaacggaa
     8881 cagcacatag tggaattgct gtattcatgg cattgaagct gtatttggac tttatcaact
     8941 tgttcttggc gtttctgaga atctttggac ggaacaacta aaaaagaaaa aaattcatca
     9001 gttggggcta taccgaatta tttggtatgg tcccgctttt tcttttcgca ttccgcagga
     9061 ttctttgtta gaatgaataa gaactttaca gtaaggagtt aagaagatga caaaaaataa
     9121 attattactc gttgatggga acagtgtggc ttttcgcgca ttctttgcgc ttcacaattc
     9181 tcttgaacgt ttcaaaaata gaaacggtct tcatacaaat gcaatctatg cattcaatac
     9241 gatgtttgaa aatgttatgc aaaaagagca accgactcat gttctggttg cgttcgatgc
     9301 aggaaaaacg acttttcgga cagaaatgta tgcggagtac aaaggtggaa gatccaaaac
     9361 acctggcgaa ttcaaagaac aaatgccata tatacgtgat ttgttgacag gactaggggt
     9421 acagtattac gaattgccca attatgaagc agatgatatt atcggtactt tggcagaaaa
     9481 agtagacaaa gatcaatttg atgtcgtcgt gctttcaggt gatcgggatt tgactcaatt
     9541 agctagtaaa gaagtaaaag tggatatcac tgtaaaaggt gtcagcgata tcgaaagcta
     9601 tacaccagaa catgtagcgg aaaaatatga tgggttgacg ccaaaacaga ttattgacat
     9661 gaagggacta gccggagatg cctctgacaa tatccccggt gtgactaaaa tcggtgaaaa
     9721 aacagctatc aagctattga aacaatatgg ctcagtcgaa ggaatttatg aacatatcga
     9781 tgaaatgaag caaagcaaaa tgaaagaaaa tctgataaat gacaaagagc aggctttctt
     9841 atcgaaaaaa ttggcgacca tcaatacttc agcgcctgtg gatgtctcta tcgactcatt
     9901 gaagtatgaa gggaaaaatc tcgataaact cgttccgttt tataaagaaa tggatttcaa
     9961 tcagttcctt tctaaattga atatcgtaga agaagaagta aaaatggatg atattttgtt
    10021 tgaagtcgtt catgaattca aagaggaaat gtttactacg gatatggcgt tgtacgtgga
    10081 aatgatgggc gacaattatc atacagagga aatcgtcgga gtagcgtggg ggacagaaaa
    10141 gaaaatttat gtcacgaacg atctatctgt attcgaaaac cgagcgttcc attcttggat
    10201 cactgatccg actcggctga aaaaagtata tgatgcgaaa cgaacttatg ttgctttaaa
    10261 ccgctatact ggaaaaacga aaggaatcga ttttgatgtc ttgctaggtg cataccttct
    10321 cgatacgaat gaaaaaagca cagatatcga aggaatagct gctcattacg gatacaacga
    10381 tatccaatca gatgaagctg tatacggcaa aggggcgaaa aaaggtttac cagaagaaga
    10441 ggaactcttt tttgctcatt tagcgcgaaa agtagctgca atcaatgcgt tgacaccaaa
    10501 attgagccaa gaactggcag aaaaaaacca agaagatctt ttccggaaaa tggaactgcc
    10561 attgtctatc attttggcag agatggaaat aagcgggatc aaagtagatg cgactcgctt
    10621 acaagaaatg aagggcgagt tctctgctcg cttaagagag atcgaacaaa aaatctacga
    10681 agaagccggc gaagaattca atttgaattc accaaagcaa ttaggagtga tcttatttga
    10741 aaaaatggga ttacccgtta tcaaaaaaac aaaaactgga tattccacag ccgtagatgt
    10801 actggaacag cttcgtgaac aagcaccaat cgtagaggat atcctcacct atcgtcaaat
    10861 tgcaaaaatt caatctacct atgtcgaggg actgctgaaa gtgatccaat cagatggaaa
    10921 agtgcatacc cgttatgtcc agacattgac ccaaacagga cgattaagct ctgttgatcc
    10981 taatttgcag aacatcccga ttcgattaga tgaagggaga aagatacgtc aggcatttgt
    11041 tcctagagaa aaagactggc tgatttattc ttctgactat tcacaaatcg agttacgcgt
    11101 acttgcgcat atctcaaatg atgaacattt gaaagctgcc ttcctagaag gtcaagatat
    11161 ccatgccagc acagcgatgc gtgtatttgg aatcgaaaag gcagaggatg tcacaccaaa
    11221 tatgcgtagg caggcaaaag cagtgaattt tggtatcgtg tatggcatct ctgattacgg
    11281 actttcacaa aatctaggaa tcagccgaaa agcagcacag caatacatcg atacgtactt
    11341 tgaaaaatac ccaggtgtca aagaatatat ggaaagaatc gtacgcgaag caaaagacca
    11401 aggctacgtc gaaacgctgt atcaccgccg tcgatatctg ccagacatca attcaagaaa
    11461 ttacaatatc cgttcttttg cggaacgaac agctatcaat acaccaatcc aaggaagtgc
    11521 tgctgatatt ttgaaaatcg cgatgatcga attggataaa cgattaaaag aaacaggtct
    11581 tcaagctacg atgctgttac aagttcatga tgaacttgtc ttcgaggtac cggaaaaaga
    11641 acttgaatca ttagataaat tggtgaagga agttatggaa caagctgttt ctcttcatgt
    11701 accactgatc actgacagca gttggggaaa aacgtggtac gaagcaaaat aaacattgtt
    11761 taagacagag caatataatg aaggtgaaaa gaaaaaatgc cagaattacc agaagtagaa
    11821 accgttcgta aaggattgga acggttagta gtaggaaaaa cgattcaaaa agttcaggtc
    11881 ttgtggccca agatcatcga gcaaccagag acacccatct ttgaggcttc tcttgttggc
    11941 gaaaccatcc aatccattgg acgtagaggg aagtttttaa tttttcatct cgatcattgt
    12001 gaactgatct cacacttgcg aatggaagga aaatatcaat ttacgaaaga aaatacacca
    12061 atcgataaac atacgcatgt acttttcttt tttgaggatg gaagccaact tcgctacaac
    12121 gatgtacgaa aatttggtcg tatgacaatc gtcgaaaaag gagcaagtgc aacttaccga
    12181 ggaatcatga aattgggacc tgagcctttg ccagatagct tcttattagc agatttcgca
    12241 aatggactga aaaagtccca caaagcaatc aagccattgc tgctagacca acgtctagtg
    12301 actggtctag gtaatattta tgtagatgaa gcattatggg aagcgaagat ccatccagaa
    12361 caaccggcga acacactgcg ccccaaagag gtagaacaat tacgtctatc gatcattgat
    12421 gttttggatc gtgcaataga agccggaggt accacgattc gcagttattt gaatgcatta
    12481 ggagaatcag gcggatttca agtagcactg catgtctatc agcaaactgg caaaccatgt
    12541 atacgttgtg gaacgccgat cgtgaaaaca aaagtggcac aaagggggac gcactattgt
    12601 ccgaattgtc aaaggctaaa aatcgcaaag taggatttgt tttaggtttg acgggtggaa
    12661 tagctaccgg taaaagtacg gcagccaaag tctttcagtc tcatggattt cctttgatcg
    12721 atggcgatgt gattgccaga gaaactgttg agcctggaac gcttgctctg aggaaaattg
    12781 tatcagtgtt tggccaagag gtattgcagc ctgatgggca actagatcga gggaaacttg
    12841 ggatgattgt ttttccatca aaggaaaaac ggcaaaaact tgatcagcta ttagatccgt
    12901 ttatccgaaa agcgataaaa gatcaaatcg cctctctaag ttccaatcat cctttagtaa
    12961 ttgtagatat cccattgctc tacgaaggtc attatgacca ctatatggat gcagtagctg
    13021 tcgtttatac tacgccagaa actcagcttc agcgtttgat gaaacgtaat caactgacgc
    13081 tagaacaagc gcagcagaga attagtagtc aacttccaat cgaagaaaaa aagcagcgtg
    13141 cggatatttt gttcgacaac aatggaacaa aagaaatact tgtgggacaa atcgaaaatt
    13201 ggctttttgc taatcgcttt ctttgagtat ctgtgtcaat tttgctatcc tgagtatata
    13261 gaaagtgagg gatagcgatg ggatcattaa aaaatcaaaa ggataaagtc gtaggtaacg
    13321 caaaagaaaa aatcggcgag atgctcaata aagaaaatct aatcgatgaa ggtcgtacgc
    13381 aatcagaaac agcagaagaa aaagaaaagc agtaccaagc aagaatcgat gaaatagaag
    13441 accagcaggc agaagaagaa gcgaagcggc aagatttgac tcaagaacga aaaaatccga
    13501 cacctgatcg tagtaaaaca ccgctcccag aaagtgacca cattcccgat gagcaaaaaa
    13561 tcgaggaaca gcgaatgaaa cactatacag gaatcgaaga gaagttataa aatagtaaaa
    13621 aatcgagatg tgacttgaag cagatgagaa ggtgttcatc tgcttcaagt tttttgtgct
    13681 agatgactaa atcagctttt gtgtaagtta tttctcagta atcaaggtga tcagtattgg
    13741 aaaattgaaa tttagtaagg acaggggaca caattacgca tgaaagtaga aaaaattgtt
    13801 cttatatccg tgttttttgg gtaggacagt cgcgtttgtg atttgtttcg tgttataata
    13861 caactagttg taaagaaaga cacatccttt atataaaatg cgatgcaaga ggtgagacaa
    13921 aatgagatgt cccagatgtc aacataataa ttcacgagta atcgacagcc gtcaagcaga
    13981 tgatggacgt gcgatccgtc gccgtcgcga atgtgagaat tgttcgttcc gattcactac
    14041 atttgaacga attgaagctg ctccgttatt agtaatcaag aaaaacgggg aacgcgaaga
    14101 gttcaatcgc gataaaattt taagaggact catccgttca gcagaaaaaa gacctgtagc
    14161 tatggaacaa atggaacaaa tcgtcaacca tgtcgaaaac cgcgtacgca gtctcggaga
    14221 aaacgaagta tcgacgaatc taattggtga atatgtgatg gaagatttag ttgatttaga
    14281 cgaaatcgct tatattcgtt ttgcaagtgt ttatcgccag ttcaaagata tgtctgtttt
    14341 cttaaaagag ttgcaagata tcgtggataa agcaaaagaa aataataaag agaataataa
    14401 agaaaacaat taagaaacaa aaaggaggca tttccttgga aaatgcttgg aaagagcttc
    14461 aaccaaaaaa tatttaccaa ggaagaaaag cggctccgct gagagaagac agcaagaacg
    14521 ccttgctgta tctttatcag ccactaatcg gcggagaagc cttgagtatc tatttcaccc
    14581 tgttatcaga aatctctcaa gaatctggca aaggtcctga agggatgcat gcagatctgc
    14641 ttagtagtct aggctgcggt atcccgcaat tttatgatgc aagaaaaaag ctagaaggaa
    14701 tcggcttact ggatgtttat tttaaagaag atagtgatct gggacctcgt tttatttatg
    14761 agctgttaga accactcatt cctaaagcat ttttcaaaga ctctgttctg gcttttcttc
    14821 ttctggaaaa agttggtgaa cgacgattcc agaagcttac ccaattcttt gaacctaaaa
    14881 ggcatacagc agagggatat caaaaaatca caaaaaaatt tttagatgtt tatcgcttca
    14941 atgaagcagt gtacgctgct aatcaagaaa acatcgaatc gctgaataaa tcttttgaag
    15001 aatctgaatc agtagcatta cttgaagcag attcgacatt cgattggcag tttttgaatg
    15061 attggctggc aaaaaaacat attgcaagtc ttgatgatcc aatcatcaaa caagtcgaca
    15121 tgtatcatca gctttacggt ttcgatgaat tgacacttgg cgaactgatc agtcaatctt
    15181 atgactttag tgaacaaaaa gtatctgaaa aagaactgca aaaagccatt ttggcaatgc
    15241 agagacgtcc ggtgaagcaa acaaaagaaa cagttgcggt ttctgaaaac agtaatgaac
    15301 cctcgtcaaa cgaacatttg acaaacgcag cgcttcagct gataaaagaa gcacagagca
    15361 ttccaccgat gaaatatctg gaagcaatca agtcagaaaa aggcggatat gtttctaaac
    15421 aagaaaactg gttactgcaa gatcttgttt ctcaatcagg tctttccagt agcgtgatca
    15481 atgttttgct gaattatgta ctagtgatca agaataatgc tagcttaaat gccagttatg
    15541 taaatacagt ggcaaatgaa tgggcacaaa agaaaatcgc cacagcagaa gaagcgatcc
    15601 agcacattcg gcaaataagt aatcaagcaa aacaatcaaa acaaaagaag caaacaaatt
    15661 atcaaactgg caagcgaaat gttcgacggg aaaagcttcc tgattgggtc aaccagccaa
    15721 aagatgaaaa acagatcagc agcgagaaaa aagcagagat tgaccgccgt tttaaagaat
    15781 acctggcaaa aaaggagggt gatatctagt ggaagacgta ggcaaagaat tgaagaggat
    15841 catcagcaaa cgcaattatc aagaaaagta tgctgaaatg atcgctgaag tcttaaatga
    15901 tgcggatgtt caagcttttc taagcgcaca ccaagaagaa ttgacacaag cggatattga
    15961 aaaaagttac gctaaattgt atgaattcgt gcaagaaaag cgtaaatatc aaaaaaacga
    16021 tccaacgatg atcgctcccg gctatgagcc gaagctgacg ttgaattttc attatgtgga
    16081 tgtcacttat gtaccaacgg aagcattgtt agcacggcaa agacaagaag aaatcagaag
    16141 ccgagtaaaa gcaatggata tgccaaaaga tattcaatcg gcttcttttg aaaactttga
    16201 acgtacagat ggacgaggat atgcgagctt agaagcccta gattttgtag agaaatatac
    16261 tgctgatcca aaagggtatc ataaaggact gtatctagta ggaagttttg gtattggaaa
    16321 aacatattta ttaggagcaa cagcgagaga attagcgatt gctggattca ccacgacgct
    16381 agtccatttt ccgacatttg ccgttgagat gaaacaggca atcggaaaag atcaagtggc
    16441 agaaaagtta gatgcagtga aacgttcacc tattttgatg atcgatgaca ttggtgcaga
    16501 tgcgatgagt agttggatac gagacgaagt attcggtgtt atccttcaat atcggatgca
    16561 ggaacagtta ccaaccttct tctcatcgaa ttttacaatg gatgaattgg aacagcattt
    16621 gtccgtcact caacgtggag atgaagagcc gctaaaagcg aaacggataa tggaacgcat
    16681 acgttatttg acaaaagaaa tcgaaatgac tggtcgcaac cgacgcaatg gttgaacagt
    16741 tgacaagtcc aactgacatt tgacctagta gtaaaagcag gtcagcgaat cattatatgg
    16801 acagaaatga ccggagacaa tcaccgcaac aagtaatgca gaaacttaaa cggaggattt
    16861 tttatgaggg ctattttttc tagtatatct gtagttggtc tattttttct tttagcaggc
    16921 tgcggtagcg cagatagcaa cacaggttct gttgattcga ttgatgcggg tcaaacagta
    16981 aaaacaagca gtacgaaaac ggagagacaa agcatatttg tcggaacgat tgaaagtatc
    17041 actcaagtag aagcagaaga agaaacgctt cagttgaaac tcgtcgatgt catggccaaa
    17101 gaagatccag cgaatatggg aacgtcattc agcaatgacg gagtgatctt aaatgctact
    17161 gctgaaaaaa taagcgtgga cataaatgaa ttgaaaatcg gtgacaaagt ccaatttaca
    17221 ttagttgaga tgccgatcat gacgatgtct atcccaccgc aagttcctgg agattcgatt
    17281 attgatgttt cattgaacaa ataaccaata acattttttc aaaaataaga aaatgttgtt
    17341 ttatagaacg aaaaggtata ttttttaagg agatatggga atttttttat tcctagtctc
    17401 cttattttat ttggaaaatg ctataattat ctagaaggag tgaagctatg aaccatttag
    17461 atttatctca gcgcttagcc gcagtgggtg actttgtccc tgaatccgct cgtttagcag
    17521 acattggatc agatcacgct tacttacccg ttgcattgat attaagagga aaaatcgaat
    17581 atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc agcacgtaag caagtcaaaa
    17641 aagatggatt agaagaccgt atcaccgtac gccttgcaaa tggtctagat gctatccagc
    17701 cacaagatgg tattacagct gtcacgattg cgggtatggg aggttcactg atccgagata
    17761 tcctagaagc tggcaagttg aatcaacgct taagcggaca agaacgtttg atccttcagc
    17821 caaacatcgg agagcgtaca ttacgatctt ggctgcaaga aaatcagtat aaaatccaat
    17881 cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat cattgtagcc gaaaaagaac
    17941 aaaaaccagt tacttacaat gaaatggagc tgatgttcgg gccatttctg cttgaagaga
    18001 aaagtgagat cttcaaatca aaatggacaa gagagcttca acaaagaaaa gctatccgtg
    18061 aacagctcaa acaagcagct aatcaagagg cacgtcaaaa acaaatcgag caggaaatcc
    18121 aaatgattga ggaggtttta aagccatgac aatcagcgga cgcactttca tccaaaagtt
    18181 tgaaagctat tgtcctcagt ggctagctga agaaggagac ccagtcggac tgcatatcgg
    18241 aacattggat aagccaatcc aacgagtaat gatgacatta gacgttcgtc cagaagtggt
    18301 cgaagaagca atcaagaaaa aaatcgattt gctgattgct aaacatcctc caatctttcg
    18361 tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa atgtatgcag atttgttgaa
    18421 acatgatatt gccgtctatg cagcacacac gaacatggat attatttggg atggactgaa
    18481 tgactggttt tgtgaacttc tcggtattga agtggagagt tatttagtaa agactcatga
    18541 gattcattat aaaaaactgg ccgtttatgt acctgtggat catgcacaga aaatgcgtga
    18601 agtattagct gcagctggtg ctggtacaca aggtgattat accggtacaa gttttacctc
    18661 gattggacac ggtcgattta ctccggaagc tggtgcacaa ccagcgattg ggaaagtagg
    18721 gaagacagaa caagttcaag aggcaaaagt tgaggttatt ttgccggaaa ctatcgaaaa
    18781 acaagtgatt caagccatgc ggtcagctca tccttatgag gaacctgctt atgatctttt
    18841 cgctatagat gaaccagtcg aaatgtttgg gttaggacga gtaggtgaat tgccgcaaga
    18901 aatttctata gaggcatttg tagaacaagt aaaagaagcg ttccaactag atggactgag
    18961 aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc attgcgattt gcggtggaag
    19021 cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca gatgtctata tcacgggaga
    19081 tatctattat catacggctc atgatatgca aagcgctggg ttgattgcaa ttgatcccgg
    19141 tcattatatt gaatcgttat gcaagcaacg atttatcgag aaatttgaat cttggaaaca
    19201 agaagaaaat tgggatatta actttttcgt ttctgaaaca aatacaaacc catttcaatt
    19261 taattaaaag gagagaatac tatgtacgaa aatcttttgc cacgtttttt acgatatgtc
    19321 aaaacagaaa cacgctccga tgcaacaagc aaaacgactc catcgactca gacacaagtt
    19381 gcttttgcgc aagaattgaa aaaagaatta gaagagttag gcttagctga tgttttctat
    19441 aatgaagata acggattcgt tatgggaaca ttaccaagca atgttgatca tgaagtgcgt
    19501 tccattggat ttatttccca catggataca gcagatttta atgcagtagg agtcaatcca
    19561 caaatcattg aaaactatga tggcgagtct acgatcgtac tagatgcgga agaacgctat
    19621 acattgaata caaaagattt tccaaatttg aaaaattatg aaggccacac attgatcaca
    19681 acagatggca gtacattact gggagcagat gacaaatcag ggattgcgga aatcatgact
    19741 gcaatggaga tcttgatcaa taatccttct atccctcacg gtaccatcaa agtggctttt
    19801 ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg atgttgagca attcgatgta
    19861 gattttgctt atacagtcga tggtggtcct ttaggtgagt tgcagtatga aacgtttagt
    19921 gctgctcaag caaatattac gatccaaggg aaaaatgtac atccagggac ggcaaaagat
    19981 acaatgatca atgctctaca gttagcaatc gattttcata atcagcttcc agccgatgaa
    20041 gttccagaaa aaacggaagg atacgaaggt ttcttccatt tgatggcatt ggatgggaat
    20101 cctgaagaag cttctatgag ttatatcatt cgcgatcatg atcgtgaaaa attcgaagca
    20161 cgcaaagcac aaatcacaca tatccaagaa gagttgaatc agcgatttga tcaagaacga
    20221 atcaaagtag atatgtatga tcaatattac aacatgaaag aaatcattga aaaagatatg
    20281 agcatcgtgg aattagcgaa acaagcaatg atcgagcttg gtatctctcc agtgatcgaa
    20341 ccagttcgtg gaggaacaga tggttcaaaa atctcttaca tgggtattcc gacgccaaat
    20401 ctatttgccg gaggagaaaa tatgcacgga cgattcgaat ttgtttcttt acaagtaatg
    20461 gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga acacaaagag ttaaaatcga
    20521 aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg aatgtcatct gtctaaggct
    20581 aaagggcagg acaatggaat tggttgtccc gccctctttt ttgaagagta aataggaggt
    20641 aaagaatgga aaagctgcta gttttttata acaaaagttc agggaaagat gagggtgaga
    20701 aattagcacg ctggtttcaa gattatgtaa aagaaaagca gccagaatta tatgtagaac
    20761 tagctgaaac tggcccatcg attgatgatg cggaattagt caagaaagca gaagccatag
    20821 aagctgatac tttagtaatt ataggtggag acggcacgat tcatcatatc gtccaagcgt
    20881 tccaaaaaac attatctcaa taccatgtcg gattacttcc aggtggaact gtcaacaacc
    20941 ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc tgctgacgtg atcctggaag
    21001 gcaatgtacg gaaaatagac tatggtcaag tcaatgatga agtcattatc tctacactga
    21061 caatcggaat cttagcagat acagcagttt ggatcagtca agaagaaaaa cagcgttacg
    21121 gctcgtggat ctttatcaag aagtttttta agttattgct gaagaagaaa aaatatcatt
    21181 tggatttaga aacagacgag gagcattgga aaggaaaatc ccaactgttg acagtcacga
    21241 tgtctaactc cgtgggcggt tttacaaatt ttgacgactc agcgacccta gatgatggaa
    21301 aatttcatgt aacgatcgta ccttccttag atattttccg atttattttt tatttgccta
    21361 gaatcatgag aggcaaattc tatacgatcc ccggcattac ttatttcaca gccaaacgca
    21421 tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac ggatggagac acgacagatg
    21481 atcttccagt tgaattatct gttgtatcaa aaggattaac agtttattca ccaaagaaag
    21541 aagaaaaagc ttagaaatag tagttaacag aaagttggaa gaagagtaat ggatgtattg
    21601 ttacacaatc ctttgtggtt ttaccttttc attgtgaatt tttacttatt ttgtctgatg
    21661 gggtatgata aacatcaggc tagaactagc ggctggcgtg ttcctgagaa taatttgata
    21721 tttgtcagcc tgttaggcgg tggtttggga ggattgatct ctcgaaaagt tttccatcat
    21781 aaaactcgga aaaagaagtt tactatagga ttcgctgcag gtgtacttgt tgatttgatt
    21841 ctcatttatt ttttccatta aggagcagga gatgtcgtct aaaggaacga aaactaaaat
    21901 cattctgaat ctgcttttgc tgattgtgat ttttgccatt atttattatt taatcaatca
    21961 atcatttgca gatatctttg ccgaattgct gtctaccagt gttgttgttt tggcagcggc
    22021 tgttatatta gggacagttt accaaatcgc tgaagggcgc tcaatcaaag aaattgctga
    22081 acctttcgcg ccgaatttta caacgaaaga tgggttcttg acctctagct atattgcgtt
    22141 ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg gagatttatt tttataacaa
    22201 aaaaggaatg aagctatccc agggagcagg ggtgacagca ctccatatga ttatgtacaa
    22261 actggcagtg atcacttatg cattagctgg cttggtcatc caattctctc ttttttattc
    22321 taatggtcca tcaatgatct ggtttatcat cgctggtatc cttttgacga tcgtgattgt
    22381 tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa atattttttg tcagcttatc
    22441 gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg gatacatgca atcagcagat
    22501 ctactcttta agggaaacgg tccaaaccat tatccaagac aggactgcgc tattgcggat
    22561 ttatctatgg aatatgttaa aacttgtatt ttggtatgta cttccttatt tgattctgat
    22621 tgaaaaccat cctaaaatag attttctact gaccatttcg tttatcagct ttgcagtagt
    22681 gctttcaggg gttattccta cgcctgctgg tattggttcc tttgaatttg tctatttgtt
    22741 attattcaga ccagttgttg gaacagttga cgcagtttct tcgcttttac tgtatcgttt
    22801 cagttccttt atcttgccat ttctctatgg atttgtgtat gtgattgctg aacggcgaag
    22861 agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat aaagaaaatc aagggtaggt
    22921 cttttataag atctaccctt tttatcaaat caaagaaaaa cgttatggat aagataattt
    22981 tacgtttgtt tggctctttt tttatataca tacaatcaat tttgtttttt taagatttat
    23041 ataagtcgag aaaaaaagtc aaaaaactat gtgtatattt tgaccaaaat tgaccaaagc
    23101 gatataatag tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa ttaggagggt
    23161 atacctgtat gaatattgag aaaatgacta cgacgcttca agaagcgatt gctgaagctc
    23221 aacagattgc ggtcactcga aaacatcaag atatcgacat tgcccatgta tggaaaatct
    23281 ttttacaatc taatcatttt ggtcgaaatt tctatacgga tgccggattg gatgtcgaat
    23341 cttttgaaca tgaaattgat cgtctactgg atgagtatcc agttgtttct ggtggaaacg
    23401 tccaatatgg acaaaacctc agccagaatt tctttcgatt attgaatgaa gcagaccaaa
    23461 tacgtgaaag cttcggcgac gagtttcttt caacggaagt cgttatttta gcgttgatga
    23521 aattaaaaaa ttatccgtta actgtttatt tgaataaaaa cggattatct gaaaaagaat
    23581 tgcgtaaaaa catcgaagag atgagaggag gagaaagagt gacctcacaa aaccaagaag
    23641 aacaatacaa agcattagaa aaatacggcg ttgatttagt acagcaagtc aaaagcggaa
    23701 aaatggatcc aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt attctttcac
    23761 gaaaaacaaa aaataatcct gttctgatag gagaacccgg tgtagggaaa acggcaatcg
    23821 ttgaagggct tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta aaagataaaa
    23881 cgattttttc attagatatg ggtgcattga tcgctggagc aaaattccgt ggtgaatttg
    23941 aagaaagact aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt atcctcttgt
    24001 tcatcgatga aatccacaat atcgtagggg caggaaaaac agaaggaagt atggatgccg
    24061 gaaatctgtt gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt gccacaactt
    24121 tagatgagta ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc tttcaaaagg
    24181 tattagtcaa agaaccaaca gtggaagata cgatcagtat tctgcgtgga ttaaaagaac
    24241 gttttgagat ccaccatggt gttaacatcc atgacaacgc attagtagca gcagctactt
    24301 tatctgaccg ttatattact gatcggttct taccggacaa agcaatcgat ttgatcgatg
    24361 aagccagtgc aacgattcgg gtagaaatga actctatgcc aacggaatta gatcaagtta
    24421 ctagacgttt gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa tcagatgatg
    24481 caagtaaaaa acgcttgaaa aatttgcaag aagaattagc tgaacttcgt gaagaagcaa
    24541 atgcgatgaa gatgcagtgg gaaacagaaa aagaagaagt caattctgtt tcagcaaaac
    24601 gtgctgaaat cgataaagca aaacacgaat tagaagatgc agaaaataac tatgatttag
    24661 aacgcgctgc tgtattgcgt catggtacga tacctcaatt agaaaaagaa ttgaaggaat
    24721 tagaagctaa agcaaaagac agcgagatta agatggttca agagtccgtt accgaaaatg
    24781 agattgctca agtcgttggc agattaacgg gtatccccgt aacgaaactt gtagaaggtg
    24841 aacgagaaaa actaattaaa ttgaatgaaa cacttcataa acgagtgatt ggtcaagatg
    24901 aagcagttga tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg caagacccaa
    24961 atcgtcctct aggttctttc cttttcctag gaccaactgg tgtcggaaaa accgaattag
    25021 cgaaagcgtt agctgagaac ttgtttgact cagaagatca tatggtacgg atcgatatga
    25081 gtgaatatat ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca ggctatgtag
    25141 gttacgaaga aggtggtcaa ttaacggaag ctgtcagacg aaatccttat acaatcgtct
    25201 tgttggatga aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta caagtcctag
    25261 atgacggacg cttgactgat tcaaaagggc gagtagtaga tttcaaaaat acggttctga
    25321 tcatgaccag caatatcggt tcacagttac tgctggaagg cgtgactgct gatggaacga
    25381 ttccagaagc agtggctgaa caagtcaaca cgctattacg tggaaacttc aaaccagaat
    25441 tcttgaaccg aatcgacgac acgattttat ttacaccatt gagcctagac aacgtgaaag
    25501 gaatcgttga caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa gaaatcctgc
    25561 tgacaatcag tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa ccagcatatg
    25621 gtgcaagacc attgaaacga tttatcacta aggaagtaga aacaccacta gctaaagaaa
    25681 tcgttgcagg acatgttatg cctaaatcaa aagtaacgat tactttactt gatggacaac
    25741 ttcatttcaa aacagaagag ctagaagaaa tcgtctaatc ttccattgag actataaaag
    25801 aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa cagtgttcca
    25861 gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt ctgtcacaac
    25921 ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta acgctcatct
    25981 tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt atttgtcagc
    26041 aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc atcagaagca
    26101 gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca gaactcccaa
    26161 aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca aggactggtt
    26221 tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag agctgtcagg
    26281 accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact gatcaaatgc
    26341 atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa aacgattgct
    26401 gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat caatgcaatc
    26461 cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt ttgtgtgata
    26521 aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc tatttgcacc
    26581 tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta cttctgaaag
    26641 caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta ttgtatataa
    26701 gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt ttttcgggtg
    26761 ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc ctttcgcata
    26821 agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta gtaaagcaat
    26881 cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc gactgtctgc
    26941 ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg aaagctcaga
    27001 agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac ttttagcaaa
    27061 cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa aaataatcgc
    27121 taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt tcttcatctg
    27181 tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc atcaagcaag
    27241 tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag acacggactt
    27301 gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg acaggatctt
    27361 caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga aggaattctt
    27421 ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc tgactatctt
    27481 caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag tagatcatgg
    27541 aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca agatagaaca
    27601 tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct cttggttcat
    27661 ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa cgccaatact
    27721 cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca aatttgtcat
    27781 aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca cttactggga
    27841 tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac atgatttccc
    27901 atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc cattgcttat
    27961 tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca tggttagcat
    28021 attctggata agttccaact ccagtcacac cacccatttt ataacgtgca ccgtgaatat
    28081 tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg aaccatccga
    28141 acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt ttattctcaa
    28201 acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc aattcattga
    28261 attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta aaatcttttt
    28321 gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat tctacaaaaa
    28381 aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt tggtataatg
    28441 gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat gaaagaacgc
    28501 caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa atctacattg
    28561 gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca agatcagctg
    28621 ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa tgctgtagaa
    28681 ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga caccccaggg
    28741 cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg tgccgtttta
    28801 gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta tttagcgtta
    28861 gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc agcagatccc
    28921 gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga agctgtatta
    28981 gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt ggaatatgtt
    29041 cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga ctctatttat
    29101 gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt caaacccgga
    29161 gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt cggggtcttc
    29221 tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta tatcacagca
    29281 agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc agacaatcct
    29341 gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg tggtctttat
    29401 ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact gcaattgaat
    29461 gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg gttccgctgt
    29521 ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg ggaattcaat
    29581 ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac ggatggtacg
    29641 actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca ggatgtagaa
    29701 gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc agtaatggaa
    29761 ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga ttaccgagta
    29821 aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga caaattgaaa
    29881 tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca aaaaagcaag
    29941 ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag ctttatcgtt
    30001 caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa aaaactaatc
    30061 ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat cgttgcccgt
    30121 tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg agatgtttct
    30181 cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa acaaatcggc
    30241 tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggacga ggacgaaccg
    30301 aagaaatagc aagggtttgt ttgaaaacag tgtaaaaaat aaaccatgtt tacatggatt
    30361 tgccaccggt ttgccaccgg tg
//
LOCUS       DAYYDS010000028        29203 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000028 DAYYDS010000000
VERSION     DAYYDS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.028"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1007
                     /locus_tag="ACHA02_001219"
     CDS             <1..1007
                     /locus_tag="ACHA02_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8669579.1"
                     /translation="FYTSINIPYGSMASAITSVAEERTALSTFRSIGATLAGMFIGVL
                     TPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKSDESQLKVS
                     LLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIVLMNFINPI
                     LMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLVLTSVGYMG
                     LGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVGGWTLSIIG
                     YDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENYNLLNQKHN
                     "
     gene            1018..1812
                     /locus_tag="ACHA02_001220"
     CDS             1018..1812
                     /locus_tag="ACHA02_001220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8669580.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            1825..3591
                     /locus_tag="ACHA02_001221"
     CDS             1825..3591
                     /locus_tag="ACHA02_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8669581.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            3619..5427
                     /gene="uidA"
                     /locus_tag="ACHA02_001222"
     CDS             3619..5427
                     /gene="uidA"
                     /locus_tag="ACHA02_001222"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8669582.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            5599..6000
                     /locus_tag="ACHA02_001223"
     CDS             5599..6000
                     /locus_tag="ACHA02_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8669583.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            5994..6239
                     /locus_tag="ACHA02_001224"
     CDS             5994..6239
                     /locus_tag="ACHA02_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8669584.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(6563..6784)
                     /locus_tag="ACHA02_001225"
     CDS             complement(6563..6784)
                     /locus_tag="ACHA02_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669585.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(6946..7018)
                     /locus_tag="ACHA02_001226"
     tRNA            complement(6946..7018)
                     /locus_tag="ACHA02_001226"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6983..6985),aa:Lys,seq:ctt)
     gene            7094..7324
                     /locus_tag="ACHA02_001227"
     CDS             7094..7324
                     /locus_tag="ACHA02_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8669586.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            7368..8537
                     /locus_tag="ACHA02_001228"
     CDS             7368..8537
                     /locus_tag="ACHA02_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8669587.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            9111..9680
                     /locus_tag="ACHA02_001229"
     CDS             9111..9680
                     /locus_tag="ACHA02_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8669588.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            9667..10527
                     /locus_tag="ACHA02_001230"
     CDS             9667..10527
                     /locus_tag="ACHA02_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8669589.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            10717..11421
                     /gene="yycF"
                     /locus_tag="ACHA02_001231"
     CDS             10717..11421
                     /gene="yycF"
                     /locus_tag="ACHA02_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8669590.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            11426..13261
                     /gene="walK"
                     /locus_tag="ACHA02_001232"
     CDS             11426..13261
                     /gene="walK"
                     /locus_tag="ACHA02_001232"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8669591.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            13258..14574
                     /gene="yycH"
                     /locus_tag="ACHA02_001233"
     CDS             13258..14574
                     /gene="yycH"
                     /locus_tag="ACHA02_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8669592.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            14575..15444
                     /locus_tag="ACHA02_001234"
     CDS             14575..15444
                     /locus_tag="ACHA02_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8669593.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            15499..16308
                     /locus_tag="ACHA02_001235"
     CDS             15499..16308
                     /locus_tag="ACHA02_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8669594.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(16411..17040)
                     /locus_tag="ACHA02_001236"
     CDS             complement(16411..17040)
                     /locus_tag="ACHA02_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8669595.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            17210..18502
                     /locus_tag="ACHA02_001237"
     CDS             17210..18502
                     /locus_tag="ACHA02_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8669596.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(18578..19240)
                     /locus_tag="ACHA02_001238"
     CDS             complement(18578..19240)
                     /locus_tag="ACHA02_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8669597.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            19465..19749
                     /gene="groES"
                     /locus_tag="ACHA02_001239"
     CDS             19465..19749
                     /gene="groES"
                     /locus_tag="ACHA02_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8669598.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            19800..21425
                     /gene="groL"
                     /locus_tag="ACHA02_001240"
     CDS             19800..21425
                     /gene="groL"
                     /locus_tag="ACHA02_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8669599.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(21514..22653)
                     /locus_tag="ACHA02_001241"
     CDS             complement(21514..22653)
                     /locus_tag="ACHA02_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8669600.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(22764..23348)
                     /locus_tag="ACHA02_001242"
     CDS             complement(22764..23348)
                     /locus_tag="ACHA02_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8669601.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(23475..23906)
                     /locus_tag="ACHA02_001243"
     CDS             complement(23475..23906)
                     /locus_tag="ACHA02_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8669602.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(23924..24244)
                     /locus_tag="ACHA02_001244"
     CDS             complement(23924..24244)
                     /locus_tag="ACHA02_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8669603.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            24544..24684
                     /locus_tag="ACHA02_001245"
     CDS             24544..24684
                     /locus_tag="ACHA02_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669604.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            24982..25239
                     /locus_tag="ACHA02_001246"
     CDS             24982..25239
                     /locus_tag="ACHA02_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFM8669605.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(25210..25518)
                     /locus_tag="ACHA02_001247"
     CDS             complement(25210..25518)
                     /locus_tag="ACHA02_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669606.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            25596..26342
                     /locus_tag="ACHA02_001248"
     CDS             25596..26342
                     /locus_tag="ACHA02_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8669607.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            26357..26560
                     /locus_tag="ACHA02_001249"
     CDS             26357..26560
                     /locus_tag="ACHA02_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8669608.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            26665..26964
                     /locus_tag="ACHA02_001250"
     CDS             26665..26964
                     /locus_tag="ACHA02_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669609.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            26964..27146
                     /locus_tag="ACHA02_001251"
     CDS             26964..27146
                     /locus_tag="ACHA02_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669610.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(27152..27628)
                     /locus_tag="ACHA02_001252"
     CDS             complement(27152..27628)
                     /locus_tag="ACHA02_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669611.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            27681..28406
                     /locus_tag="ACHA02_001253"
     CDS             27681..28406
                     /locus_tag="ACHA02_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8669612.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            28580..28921
                     /locus_tag="ACHA02_001254"
     CDS             28580..28921
                     /locus_tag="ACHA02_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669613.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            28914..>29203
                     /locus_tag="ACHA02_001255"
     CDS             28914..>29203
                     /locus_tag="ACHA02_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8669614.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT        10099 a         4570 c         5769 g         8765 t
ORIGIN      
        1 ttttttatac atctattaat attccttacg gttcaatggc atcagctatc acttctgttg
       61 cagaagaaag gacagcgtta tcaacatttc gtagtatagg agctacctta gcaggtatgt
      121 tcattggcgt gttaacacca atttttattt atacaagaga tagttctgga gctcaaattg
      181 tacgtggtgg atcaactttt acaattgtgg caggagtatt ttcagtgtgt gcagttattt
      241 gttacgtaat atgttataaa ttaactgtag aacgggttaa aagtgatgaa tcgcagttaa
      301 aagtttcatt gttgaaatct ttaaaaggat tagctaaaaa tagagcgttg ctttctataa
      361 tagctgcagc tatttttttg ttgcttgctc agcttttaac aatgagtatg aacaattatg
      421 tatttccaga ttattataat aatgcacagg gaatagtttt aatgaacttt atcaatccta
      481 ttttgatgtt ggtacttgtt tcacctgtaa gtttaatttt cagtaaaaaa tacggtaaaa
      541 aagaattggc gtcagtaggg atgttcttct ctgctattgt atatttactt ttatttttga
      601 taaagcctag taatatgtat gtattcttag ttcttacttc tgttggatat atggggttag
      661 gtatttttaa tacagtcatt tgggcgaata ttactgatgt aatcgatgat caagaagtga
      721 aatctggcca aagagaagat ggaactattt atgcagtata ttcttttgcc cgaaaaattg
      781 gacaagcttt agctggcggt gtcggaggtt ggactctttc tataataggg tatgattcgt
      841 tagcaaaagt acaaagtgga gaagttttac aaaagctgta taactcttca acgttgattc
      901 cagcagtttg tttctttgtt gtaggattga ttttattttt cgtatatcca ttatctaaag
      961 aaaaagtatt agaaaattat aatttgctaa atcaaaaaca taactaggag aaaaaacatg
     1021 aacagttgga tattttgtat tgattcagat ggttgtgtca tggatacaat gacttataaa
     1081 cacaatctct ttttcgggcc aattgctgca gagatttttg atgttcatca tagggaaaaa
     1141 ttcctacagg attggaataa tattaattta tattcagaga ctcgtggaat aaacagattt
     1201 ttaggtttag ttaaaacatt agaaaaaaat gatgtaccat cgattaatcg attaaagcac
     1261 tgggtatctg taacaaaaga attgtctaat ccatctttga agagggaaat agaatactct
     1321 cagtcagagg atttattaaa agctttaaaa tggtcggaag aagtcaatcg acagattagt
     1381 caagctaaag gattagataa acccttttta agtgctagag atactataaa agctctgaaa
     1441 aaatatggga agataattat tgtaagctca gctaataagg aagctattca agaagaatgg
     1501 gaaagacatg aattattatc cttagttgat gaactgtgtt gtcaagataa aggaaaaaaa
     1561 gaagacatta ttcgaagtgt aattgaaaat ggttgtgatt tggataagat attaatgatt
     1621 ggtgattctc ctggtgatct agaagctgca aataagaata aagttttctt ttatcctatt
     1681 ttggttaata aagaaaaaga atcatgggaa aacttaagaa ctgatatttt atttaaatgt
     1741 ttatcgggag agtatataga tattgaacaa aaatatatag atactttttg gaaaaatctt
     1801 actaataagt agaggagaaa gaaaatgact ataaaagtag acttacgaaa aaaaccatat
     1861 catttagatg aagatgctat ttcttggatt gaagagacga ttgatgctat gactttagaa
     1921 gaaaaaatag gtcaattatt tgttaatatg ggatctagtc gtgataaaaa ttacttaagt
     1981 agtatggtca atcagtacca tattggagct gtacgatata atccaggaac tgctgatgag
     2041 gtatatgatc aaaactatat cctacaaaca gaaagcaaaa ttcctttatt aattgctgct
     2101 aatacagaag caggtggtaa cggagcttgt acagatggta ccgaagttgg tgtagaagta
     2161 aaaattggtg caacaaatga tgcgaaatat gcttatgaat tagggagagt ttctggaatt
     2221 gaagcatcgg ctattggatg taactggagt tttgctccta ttgtagatat taatagaaat
     2281 tggagaaatc ctatcatttc tagtcgatct tttggacaaa gccctgataa agttttagaa
     2341 cttagtttag cttatatgaa aggtattcaa gagagtggaa ttttgcctgc agctaaacat
     2401 ttccctggtg acggtattga cgaacgagat caacatctat cttttagtat taatagttta
     2461 agtacagaag aatgggataa ttcttttgga aaagtatact caggcttaat agaagctgga
     2521 ttaccttcaa ttatggcagg tcatatccat atgccagctt attcaaaaca ttttaatctt
     2581 gatctgaaag atgaagattt acttcctgca actttatcaa aagaattaat cactgactta
     2641 cttagaggaa aactcgggtt taatggatta gtagttactg atgctagtca tatgttagga
     2701 atgactgggg caatgaaacg gtcagaatta ttgcccactt ctattgcggc tggatgtgat
     2761 ctattcctat tttttaatga tccagatgaa gactttaatt atatgatgga aggttattac
     2821 aaaggaatta tttcagaaga gcgtttacgt gatgcattaa gaagaatttt aggcttaaag
     2881 gctaaactag gtttgcataa aaaagaaaag aataaaattt tgttaccgaa acaagaagct
     2941 atggaaaaaa ttggtttaat tgaaaataaa tcaattttta aagaggtagc tgatcaagca
     3001 attacactag ttaaagataa acaagatatt tggcctattg taccagaaaa atataaaagg
     3061 attttactgg tagatgtaca tggggtaaaa ggtgggtttg gtgaagtagt tgctggaaat
     3121 agacctcagg ccattgattt attgaaacag attttgtcag caaaaggatt tgaagttgaa
     3181 aaatacattt cacctgaaga agaattgaaa ggattatcgc aagaagaaaa aattaaaaat
     3241 atagctggaa tatatgctgc taagagacca atcaaagatt taactgaaaa atatgacttg
     3301 attattaata tagctaatgt taacccaggt acagttcaaa gaattgtttg gccagcaagt
     3361 aaaggtactc cggatattcc attttatatt catgaaattc caacaatttt tgtatctgta
     3421 cagtgtccgt ttcatttagc tgatgtgcct caagtaaaaa cttatatcaa tacttatgat
     3481 ggaaaacaag aaactttaga aatattagtg gacaaactaa tgggcctttc tgaatttaag
     3541 ggaataagtc caatagatgc ttattgtggg ttaaaggata catttattta atatttaaat
     3601 tatataggag tgatttacat gttatatccc attatgacag agacgaggca agtaattgat
     3661 ttaagcggtc tttggagatt taagttagat acaaatgatg acttacaaga agaacttgct
     3721 ttagaaccaa tggttgaaaa agagagttat ccaatttcag ttccttcttc ttataatgat
     3781 ttatttgaaa cagagaacat tagaaatcat gttggatggg tgtggtatga aaaagaagtg
     3841 attcttccta aaaaattatt agaagaaaga ttagttcttc gttttggatc tgcaacacat
     3901 gaagcaaaag tatttttgaa tggaaaattg attaatcaac acaaaggagg gtttacgcca
     3961 tttgaatcag aaattaatca gtttataaaa gatggaagta acagaattac agtggctgta
     4021 aacaatgtta ttgacgatag cactttacct gtagggattt tgaaagaaac gatacaggaa
     4081 gatggagaca taaaaaaaga aaatttggtt aattttgact ttttcaatta tgcaggcatt
     4141 aatagacccg ttaaattgta tacaactccg aaagactata taaaggatgt cactattaat
     4201 acaaaaataa ttgattcaga tgcaaatgta gaatttaata tcgatacaga atggaccgga
     4261 gaaattctaa tagcaatcta tgacgaagaa gctcaactta tagcagaagc taaggggaaa
     4321 gaaggagtga ttcaacttaa gaacccaaca ctttgggagc ctctaaatgc ttacttatat
     4381 agtatgaaag ttagtttaat taacgaaaat aaagtgattg atacgtacac agaagaattt
     4441 ggcattcgta gtattcaggt ttcgaatggg aagtttttga ttaataataa acctttttac
     4501 tttaagggat ttgggaagca tgaggacacg tatattaatg gtcgtggttt gaatgaagca
     4561 gtaaatatca aagattttaa tttaatgaaa tggatcggtg ctaattcatt taggacttca
     4621 cattatcctt attcagaaga aattatgcgc ttagcagatc gagaaggtat tgtagttatt
     4681 gatgaagtac ctgcagtagg attacatttg aatttcatgg ctactttatt agatgacaac
     4741 gtagaaaaac atgatacttg gaaagaaata cggacagaaa atgctcacaa aatggcgatt
     4801 aaggaattga tagaacgcga caagaaccat ccgagtgtcg taatgtggtc tattgcaaat
     4861 gaaccagatt cggattctga aggcgctaaa gaatattttg aacctttagt tgaattgact
     4921 aaagaacttg accctcagaa gagacctgta acaatcgtta cttacttaaa gtcaacacca
     4981 gatatttgta aagtaggaga tattgtcgat gttttatgtt taaatcgcta ttatggttgg
     5041 tatgtttcag gtggagacct aaaacatgca aaatatctgt tgaaaaaaga gctagatgga
     5101 tggatggaaa gatgtcccga tacgccaata atgtttacag aatatggagc agatacagta
     5161 gctggacttc atgatacagt accaacaatg tttacagaag agtatcaagt gggttattac
     5221 gaagcaaatc acgaagtaat agataaatgt gacaattttg ttggtgaaca agtgtggaat
     5281 tttgcagatt ttgaaacaag tcaagggatt ttaagagtgc aaggaaataa gaaaggaatc
     5341 tttactagag atagaaagcc taaaatgatt gctcactatc taaagaaacg atggtcatca
     5401 ataccaaatt tcagttataa aaaataattt accttttata aggaatataa aaaaggagtg
     5461 gctgcttaat tgccaactcc ttttttttat aaaaagattt tctgtttaaa gaaggatatt
     5521 tttttctagt tttttcgaaa aaaaggcgct ttctgattgt tgtattagtg gaaaggggaa
     5581 aaaaggagca tatttatcat gcaacaaaat tacaatgaga aatctataca acatcaattt
     5641 gatgcctact gtaaaaaagt attacgaaac gaaactaagt ctattcgaaa acgaaacatt
     5701 aacataacag ataatgagga accattgacg aattcaaatg aaggtaagta ctcccaaact
     5761 cattttgatg aacaagatgt ctatttctta tttggaatgg aaatattaat ttcagatcaa
     5821 aagttatcgg cagccattga tcatttatcg gaaataagaa gaaaggttat cctactttat
     5881 tattttgcag gttttaatga tactgaaatc ggtaaaatcc tcaatatgag tacaagtggt
     5941 atctggtatc aacgaaagaa ggcagttgaa caattaaaaa tggggtatga cttatggtag
     6001 acaaccccaa aaaacggcag tatccattag ttcctctatc cattatccaa tctgctctag
     6061 aagaagatcc aatttcaatc aagatgatac tcaaacatta tcatagttat atagctaagt
     6121 tatgtttgac aaatggattt aatgaggcgg ggcaatttat tacctatgta gatgagtata
     6181 tgctaagaca gctagaaatt aaattgatag aagcgatctt aaaatttaaa attaactaac
     6241 taaaaagtcc cctttccact tattctgttt aaaagaatct tctaatagtt aaaggattag
     6301 tagattcttt taaacagaaa acctattgat cattgaaatt taaatacatt ctatcagtac
     6361 gttaacactt caaggagcat attttagttg cgccatgatt tcttactgta aagaaggagc
     6421 gataaacatt ctaaaataat ttttatagtc aataaaaagc gatgatttga agttgggata
     6481 atgatacttc cgtaaggctc gccgtaaact tggagagagg tgaaattcct atgtgctttt
     6541 gacaaaagca gctgattaag aattatttcc atatgttgat ttcaacattt tttctattat
     6601 ttttaagacc aaattcagtg aggcaaatag catcatgtaa atttagtttt tctattgaac
     6661 gttctccact ttttagtttt tttatagtac tcaaatttaa tcctgtatgt ttagagatct
     6721 catttgcagt atggttttgt aaagtttctc ggattattgt tatattagca atttcattac
     6781 gcataattat gttcctttct tatcaaaaat atcaatgact acagtgggct tttagtgaaa
     6841 aaaaggggta ctacagtctc ttttgaaaac aaaaaaatac agtatatacg ttttctaaat
     6901 gctaagcacc aacgtttaca ctgtatttaa gggtttggca cccaatgggc tgtgaggggc
     6961 tcgaacccgc gacccgatga ttaagagtca tctgctctac caactgagct aacagcccaa
     7021 attaatactt tgttattcta acacctaaaa taaaaaagtg caatacttat tatcagaatt
     7081 gagagtgaat tttatggatt ttcaagatta tgaaaaaata tttgaaagtt atccatcgat
     7141 attgaaaatt actgatttaa ctaaaatgtt aaatattgga aaaaataaag cttataattt
     7201 gctgaaagat agatcaatag aaaatataaa aattggagca aggtacaaaa tacctaaaaa
     7261 agctgtcatt gagtatatat tgaaaataca agatgaaggt aaaaatcatg ctataataaa
     7321 atagtcaaaa tttttgccga tatttcagtt taagaggaga gattgaattg aaattaagag
     7381 caaacataat tgaaaaaaga ggaacattac aagtagttgt atcttataaa gactctaacg
     7441 gaaaaattag acaaaagtgg aaagatacaa aattaaaaaa gaaaggaaat aaaaaacgag
     7501 cagaaaaaat tagagatgaa tttttactag aattggaaag tcaattagct atggaatgtg
     7561 aatgtgctaa tccaaattta ttgttttatg actatttgct taattatttg gaagtatcaa
     7621 aaaaacaagt agcatttaac acatatgttg ggtataagca ttacgtccat aatcgaattt
     7681 acaagttttt ttatcctaaa aaaattaaac taaataggtt aaagccttat catttacaag
     7741 agttttatca atatatgtta aatgataact gtacagcaaa tacggttatt cattatcacg
     7801 catttatacg caaagctctt caagaggctg ttattacaga attgataagt acaaatgtag
     7861 cagataaagt aagaaagcca aaaaagaaac aatttgtttc tcaagtttat aatcaacaag
     7921 agatcttgaa attacttgac attttgtcag aagaaaaact ctatttggta gttcttttaa
     7981 cagcatttta tggattgcga agaagtgaag ttttaggatt aaaatggtct gctattgatt
     8041 ttttaaacaa aaaaatcatg ataaaccatg taatcataga aaatccagag aatatgtcac
     8101 agttaataaa gaaagatcaa acaaagaact cttccagcta tcgtacatta cctttggttg
     8161 aaacgattga acgagcatta gtaaatcaag ctaaatggca acaagataat cgtattcttt
     8221 taggagatga ttatatttta gaagataatg agtacgtatt tactatggaa gatggacggt
     8281 tgatgaagcc tcaatatgta acgcatcgtt tgagtaagtt gattaagaaa aatggattga
     8341 aaaaaattcg ctttcatgac ttgcgtcatt ctaatgcttc aataatgtta gatagtggcc
     8401 agaatatgaa atccattcaa gaatggctgg gacatgcatc gtattcgaca actgctaatt
     8461 tatacacgca tttaaccgca ggagtaaaag atcgtgcagc agaagcatta gaaaacgcat
     8521 ttgggtttaa agaataaaaa aacaaaaaac aatatctttg gggtatcgct tcttgttttt
     8581 agaatttttt tgttggcaat ttgttggcag tatgttggca atgaaaaaat tcacaaacta
     8641 aaaacctatt tataaagctt tataaaactt ttttcacaaa tagagcattt ggttaagagt
     8701 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
     8761 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
     8821 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
     8881 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
     8941 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
     9001 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
     9061 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
     9121 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
     9181 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
     9241 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
     9301 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
     9361 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
     9421 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
     9481 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
     9541 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     9601 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     9661 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgttccagaa
     9721 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     9781 gtatatacag ataaaattga cattacccct gaagagatct atcaacgttt gccagaggaa
     9841 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     9901 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     9961 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
    10021 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
    10081 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
    10141 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
    10201 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
    10261 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
    10321 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
    10381 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
    10441 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
    10501 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
    10561 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
    10621 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
    10681 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
    10741 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgacgtt
    10801 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
    10861 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
    10921 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
    10981 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
    11041 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
    11101 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
    11161 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
    11221 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
    11281 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
    11341 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
    11401 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
    11461 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
    11521 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
    11581 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
    11641 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
    11701 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
    11761 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
    11821 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
    11881 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
    11941 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
    12001 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
    12061 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
    12121 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
    12181 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
    12241 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
    12301 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
    12361 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
    12421 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
    12481 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
    12541 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
    12601 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
    12661 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
    12721 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
    12781 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
    12841 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
    12901 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
    12961 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tgattttaag
    13021 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
    13081 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
    13141 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
    13201 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
    13261 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
    13321 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
    13381 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
    13441 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
    13501 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
    13561 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
    13621 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
    13681 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
    13741 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
    13801 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
    13861 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaagaagtat
    13921 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
    13981 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
    14041 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
    14101 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
    14161 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
    14221 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
    14281 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
    14341 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
    14401 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
    14461 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
    14521 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
    14581 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
    14641 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
    14701 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
    14761 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
    14821 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
    14881 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
    14941 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
    15001 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
    15061 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
    15121 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
    15181 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
    15241 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
    15301 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
    15361 aaagtggtcc aaattgagag cgtcaacgct ttcagcaata cgatcgtgac aaacaatact
    15421 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
    15481 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
    15541 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
    15601 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
    15661 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
    15721 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
    15781 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
    15841 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
    15901 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
    15961 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
    16021 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
    16081 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
    16141 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
    16201 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
    16261 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
    16321 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
    16381 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
    16441 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
    16501 cgtactgtcg ctagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
    16561 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
    16621 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
    16681 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
    16741 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
    16801 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
    16861 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
    16921 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
    16981 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
    17041 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
    17101 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
    17161 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
    17221 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
    17281 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
    17341 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
    17401 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
    17461 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
    17521 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
    17581 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
    17641 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
    17701 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
    17761 agcggttttt caacatttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
    17821 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
    17881 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
    17941 cgtcttgcaa gcgaattcat tagcaaatgc agaaatctta gcctcaggac aagagttgac
    18001 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
    18061 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
    18121 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
    18181 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
    18241 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
    18301 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
    18361 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
    18421 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
    18481 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
    18541 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    18601 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    18661 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    18721 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    18781 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    18841 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    18901 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    18961 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    19021 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    19081 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    19141 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    19201 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    19261 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    19321 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    19381 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    19441 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    19501 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    19561 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    19621 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    19681 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    19741 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    19801 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    19861 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    19921 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    19981 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    20041 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    20101 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    20161 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    20221 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    20281 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    20341 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    20401 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    20461 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    20521 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    20581 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    20641 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    20701 acgatctagg tttagaattg aaagatgcaa caatcgaaaa cttaggaaat gcttctaaag
    20761 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    20821 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    20881 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    20941 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    21001 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    21061 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    21121 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    21181 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    21241 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    21301 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    21361 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    21421 tgtagtaatt aagaaagaac cctattttaa cagggttctt tcttcttttt ttgttttatt
    21481 ttaagcaaaa ggggcaaaga aggggcaaaa atatcaaagg ctatttagtt tatcaattac
    21541 ttgttgtttg gctctttttg ttacatggtt ataaatagac agagttgtgt ttgcatcaga
    21601 atgccctaca cgctccataa tggctttaag aggtacgcct aattcagata atagtgaaac
    21661 gtggctatgt ctgaatatat gtgaggatag acttttttct aattccaact cttcctctac
    21721 tttatggagt atagcgttaa atgagtgcag cgcaagcgga gtgccacttg tagatataaa
    21781 tatatattga tcgggatctg tgggtctacc tgcaagaata ttgtcagcta tcacactttc
    21841 aatcaattct tttgcacgat tgggtaattg cacttcacgt tgcgaataag tattttttgg
    21901 agttgttttt atagcattat ccattttcac agatgtgtaa tctaaggtcc cattaatgga
    21961 aattttccca tcctcatagt ccttcatttg caaagctagc aattccccat atctcaaacc
    22021 agttaaatat agaaactcag ctattatgcc gtgtagtttt ctgcgaggat tggagtatag
    22081 ctgtttcagt atttgatcaa tttcttcttt ctccagatat tttttattca tagaaagcct
    22141 tcttttttct tcttccactt ttttaggatg gattttaact gctagcgcag ggtttctttg
    22201 aatgtatttt ctatcgattg catagtttag cataacagat agagttgttt ttgtttgttt
    22261 tgtgtagttc agtgagaggt caccaaacgt atacatatct tcaattatct tattaatgag
    22321 tgtctcatca atgtttctaa cgatcgtatc atcgcttatg tgcttagaaa catgtttcat
    22381 catcattgga accttcaaat agctagtacg tttaacatgc tgcttataat attcatacca
    22441 ttctttatac agctcaccaa aagtgatatc tgatttattg taatcttcga gtgcttcttt
    22501 aattttttta tctagaattt tctgagcttt tttccacgct tgtggtgaat tacttgtaag
    22561 tgttgtagat ttttttcgtg ttttttctgt ataaggatct acatatcttt caataaactt
    22621 gaattggcca tctttggtct gttcaaccca cacttttaac atctcctatc atttgctata
    22681 ataggcataa caaatagacc tatataggtt tgttttctaa aagcacgctc ttactctgga
    22741 cggtggggcg tgttttttat ttattataca gttgctttaa aaatagctgc tttttcaaat
    22801 gatactaaag gagagaagtg gatttctatt tctccctttt ggtttaacgt gaaatgagca
    22861 gttacatcca tttgttttcc tggagcaaca gatcccattg tattttcgtt ggcatatgtc
    22921 tctgattttt tgtcatctgg tccatatact tccacatctg tacctacagg gatatctgaa
    22981 tcgccatcat tttttacggt ataagtaatt tttactactt gtgcaggttg gttttcttca
    23041 aattgatttc tttcatcagt tagttctaca ctatttagtg tatattcagc atcaccaact
    23101 tttacagtgt caccaatctt atagaaggta tcgctttttt cttctttaga agatgaagca
    23161 gaggttgaag atttagttac tttttcgcca ccattatcac ttgctttatt actatctgat
    23221 ccaccattta aagcagaacc aataattata attaaaatta ctgctaatac ccaaaaccat
    23281 acttttttgt aaaaaggttt actaacttta tacatttttc cgtcttgacc cataactttt
    23341 tttgccattt aaatattcct cacttcttgt tataatatat ttgcgatctc agaaatgagg
    23401 tatgagtccg tgtgccagca cgggcttttt taatgttttg ctgaaatcgg tttttttaat
    23461 aattcttgat gtattcatac atattgcctt gcgtaagaat attttttctt aaaatagcat
    23521 tggcagacag cataacaagt gtatctgagc ttattctgaa tagaataata ctccataaat
    23581 ttttcgagat taaactgaga ttcatcagtc agttcgttct caatatagat atttaataaa
    23641 attagaatag ctattttatc tgcttctgtt tcaaattttg agtgaaaagt tgtagaagta
    23701 tcgtacaaaa ctgaatattc aaaatgagaa gcggtgaaat gcgcaagttc atgagataaa
    23761 tgaaaggctt ctgcagtttc gccatataga ttttcattca aaaaaatgat tctaggcttc
    23821 ggataataga agccaggctc tttcatttcc atgtaaacta ctttcaaatt gtattcgctc
    23881 aacatttctt tcaatttcaa atacatacaa cccatcactc caactattca ttttcctcta
    23941 aagctttagc aattgcaatc gctttacgca ttgtctcctt agatatttct tttccatcaa
    24001 aagaaaaaac agtatcgttt tctgataaat ccacatgttt aggggtttct ctttcttctc
    24061 tacctagaag gtagtctaca gagactccaa agtaatcagc taaccgagct aactcaatag
    24121 aattaggagt ttgtcttttc caacttccta aatatccatt cgaatacccg aaattcattt
    24181 cgagctgtct aatggataat cctttttctt ttgctaactc ctttattatt tcgtaagtat
    24241 tcattgataa atcaaccttt ctgaatgctt acaaaaaaag tttagataaa taatgtaaaa
    24301 atagttgact tatatacatt attaatctat actatgtttt gtaaacaagt ttaacaacta
    24361 aaaagacaac aaaaaatact attgattaat aaatgccaac cgccaagaaa gctttttaaa
    24421 tcaaagttta tatgtcttat ttaactatgc atttagtata gattattaat ctaataaagt
    24481 caacgcagtt ttttaaaaag ttgttaaatt tgtttacgaa tataaaagaa aggagagaag
    24541 aatatggaaa aaacagtctc ggcaaaaatc gaagatttga aattagacat actgaaacaa
    24601 gcaaaagtgg cgatggaaca cgcggtagat aaagaagact ctgccatggt tgcagccata
    24661 gcagagattt tagctcacgt ttagtcatcg tttttacggt cttctggaag ctgaccatag
    24721 ataacagcat aatgaatatc taaataagct tgaacaattt cttttggcga aattgattcg
    24781 cctttagtag atacttgtga ttcgtgattt agccatgcaa caactaaatc cgcagcgatt
    24841 tcaaacggcg ttacactttg tgtcagttgc cataaagaat ttcacaatat gtatggtcgt
    24901 ggtaataata ccaaagagca atttgaggaa tttatggaaa ttaaaaaata caaaaaatca
    24961 tttattaaag gaggctaaac aatgaacaag gaaatgagcc aaacaatcaa ggtacaaaaa
    25021 atgatagatg atctaacaca tggaattgat agccaagccg ataaaatcat caaggaattg
    25081 caaggacaaa aagttaagga tgcaaaaatg cttctcaaga ctatcaactt tgaaatgaat
    25141 ccaacaaaaa gaaagctcgc cgatgtgttg gaagaaaagt tggcttccgc tatcaacgag
    25201 caagaattac tatttgaaac cgatactttt aacagctgat gttttataga tgacagtttc
    25261 atccgaatct atatcatcgc cgagagaaaa gaatgttgct tttgacaaaa agccgataag
    25321 acctacaagc ggtcttgatg ttgcaagagt agatacccca atatcacctt gcaaggtatt
    25381 cacgtaaatt tgagaagaat agaacattcc atctttttct tcgatatttg acgcatcatc
    25441 gtatccaacg accaaagtat cggcaaatat tttgattttt tctccatcaa gcatagtaat
    25501 tacaaattct ggtttcataa attttcacct ccttaacaat tatttcagcc tgtcacactg
    25561 ataaggaaat tataccaaag aaaggaatga gaaatatgaa tacaccacaa atttttaatt
    25621 tcgagcaaaa cgaagttcga actattttag taaatgatga accatatttt gtaggaaaag
    25681 atgtagcaga cgttctagga tattcgaatc cgcaaaaagc tattcgagat catgtggact
    25741 tggaggataa gacgcagaac gattcgttca ccgtcaatgg aacagcagtt gttttgatta
    25801 acgagtcagg cctttacagt ttaatcttaa aatcaaaact tccctctgcc aaaaaattta
    25861 aacgttgggt aacgagtgaa gtccttccaa caattagaaa aacaggtagc tattcaaacg
    25921 tacctcaaag ttttgcacaa gcattgcgtt tagcagcgga tttagaagaa aagaaccaat
    25981 tactcgaaca acaaattgcc gagtacgaac caaagattag ctacttagat acgattcttt
    26041 catcgacaga tacggtagct acttctcaaa ttgcagctga ttacggaatg tcggcaattg
    26101 ctctaaacaa attgctaaac gagttaggtg ttcagcataa agttagcgga caatggatac
    26161 tttaccgaaa acatatgaac caaggataca caaaatcgca cacaagtgag ataccgaaag
    26221 ccgatggcgg cactaaagtt gtaatgaata ccaaatggac acagaaaggg cgagtgttta
    26281 tttacaactt attaatcgca gagggctatt accctcaaat ggatttagag gaaattggtt
    26341 agaaaggagt tttagtatga ctgacattgc agaaatcact caacgagata gagaaaaaat
    26401 caaagaatat gtcgaaagtt caaagttctt aacttacacc atgcttgctg aaagatttgg
    26461 aattagcaaa agctacttat ctttaatttt aaacggtaaa aagacttctg cagaagcaaa
    26521 cagaattata gattcgatta tcactatgta cgaattgtag agggaggtaa agtcgttgga
    26581 agataaaatc attcttacaa aatcagagct tcaagaaatg ttaaacatcg agtatggaac
    26641 gataattttt aggagggata ataagtgaaa aaaccaacac tttcagagct gatagaagct
    26701 gctgagaagg cagtaaaacc agacgactgg taccgacaaa gtttaatctt ggagaagttc
    26761 cacggcatgt caaaaactac tttagttgaa tactgcaagg aaatggaaac aattcctgaa
    26821 tttgcagaag gaatacttcg tccaggtcac tcaaccacat ttattcatta ccatactttt
    26881 atttggtttt taaagtggaa agacgcaaac aaatatcgtg taaaaagttt atcgccttca
    26941 gatgttttga aggaggcaag ttaatgggca aattcaacag agcactagta ttcagcgcac
    27001 cgctaatcat ctacgcttta ggcctctggg gaagcagaca agcgttgata gggacaatcg
    27061 tttacatggt ttggattttt atggggcttg atgaagctga gcacagagcg aaaaaagcca
    27121 gtcgggaggg actgactaaa atatgatttt tttatttttg ttcctttggg ctgctattgc
    27181 gtacttcatc cagctccttg ttgtcaaagt tgctcttatt tcttttagga agaacaaaag
    27241 aaaatagcgc tataaatgaa agataagtag ccgctacatt aaactgttct tttgaaaaag
    27301 ttacagtcat cggaatgata attgatattg aggaagtaat cttaattaaa aatatagcaa
    27361 tattttcaac atcgcgttgg tttttattta acattgtcca acatgtttta gcaacaataa
    27421 aaataattac acatgatatt agcacacaga ctgaaaaaag ttttagaaat gcgatgtagt
    27481 cagtgttatt gaactccttc aaaatactat ttttattcaa tgtaataaaa ggaatttttt
    27541 taattgaaga caggaaagat aaggtaataa acagaattat atatatggtt aaagtcgcag
    27601 taagaatgta attgagcttt ttcctcatag aatcacctca ctttctaacg aaaattatac
    27661 caaaaaggag agaagaaata atgcaagaat tagtaatttt gaaaaataaa gaagctgtaa
    27721 ctacgagctt acaagtggca gaaagtttca aaaagaaaca taagcatgta ctagaagcaa
    27781 ttgaatcaat aaaaagatcg gtcgaaaatt cggccaatgt tgaagatggg tccaattttg
    27841 gacagatgtt tgtggaaggg aacgagccag actcatacgg aagaagtcgg agagtttatt
    27901 tcatgaatag agacggattt tccttgctag ctatgggatt cactggaagt aaagcaataa
    27961 atttcaaact aaaatttatt gaagctttca acgaaatgga agatgttatt cggaagaata
    28021 ctgttcctca aacaattgaa gacatgatga tctatcaatt agaagaaatg aaagatgtta
    28081 aaaaagatgt ttccatgctt aaagatacta tgcgaattag cggacaacaa gagtttgaaa
    28141 ttaagcaaaa aggaaatatg aaagttatgg aagttctagg gggaaaagaa agccgagctt
    28201 atgaagaaat cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa cgtacctttt
    28261 caatcccaag atatggcgag ttacctcgta agagattcga tgatgctgtt tcatttattg
    28321 aaatgtggtt accagaaact gcgatccgca tggaaatcga tcaactgaac agacaacaaa
    28381 gacttttcgg tgatgaaaat gaatagagct gaagcgctaa gaatagggac ggtaattgct
    28441 aatcgctggt ggagacacaa taaaccaaac atcctaagcc aacaacatat cgaaaagcaa
    28501 aaagcatggc aacaaataaa aaagtgactc agccggcaag catagagtca caaaacatat
    28561 ctaaggagaa ttttagcata tgaataaaga actttccact ttagatcaat atttgactga
    28621 ttctgaatgg ggcaagtcga atatcaagga aacaaataat cgaaaaatca gacgaaatct
    28681 tttgacgaac gaagaactat catgtgatca agatgacttg ggaaattttg tgagtatttg
    28741 ggatcatgtt taccttatcc atctatcaaa acattcaaat aaacctgaat acatctatgt
    28801 catcgaagat ggcttgattg atgcgctaga agagtacaac agagataact tgattgatat
    28861 ctcttattac ggatcaggta agaaatacat tgctgaaatg gaggcagaat ttgatgagtg
    28921 aaatcaaagg gacaacgaac tttgaaaaac tttttagtcg taagttaaat aaaattctca
    28981 agaaaaaagg aaattttgat tatttatctt gggctcacgc gtgggagatt atgaaaaaga
    29041 atgatccaca ggcaacggta actattaatg agtataaaca ctacagggtt gtttctggaa
    29101 ctcatcaaga ctttcttgtt gaggaatata aaccttttct tatggatgaa acagggactt
    29161 atgtatctgt ctcagtaacg gttaaaggac atacggaaac aga
//
LOCUS       DAYYDS010000029        29168 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000029 DAYYDS010000000
VERSION     DAYYDS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.029"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..772)
                     /locus_tag="ACHA02_001256"
     CDS             complement(<1..772)
                     /locus_tag="ACHA02_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8669615.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYM"
     gene            complement(996..2936)
                     /gene="thrS"
                     /locus_tag="ACHA02_001257"
     CDS             complement(996..2936)
                     /gene="thrS"
                     /locus_tag="ACHA02_001257"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8669616.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3273..3452)
                     /locus_tag="ACHA02_001258"
     CDS             complement(3273..3452)
                     /locus_tag="ACHA02_001258"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8669617.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3499..5322)
                     /locus_tag="ACHA02_001259"
     CDS             complement(3499..5322)
                     /locus_tag="ACHA02_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8669618.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5600..7879
                     /gene="helD"
                     /locus_tag="ACHA02_001260"
     CDS             5600..7879
                     /gene="helD"
                     /locus_tag="ACHA02_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8669619.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(7972..8619)
                     /locus_tag="ACHA02_001261"
     CDS             complement(7972..8619)
                     /locus_tag="ACHA02_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669620.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8798..9517)
                     /locus_tag="ACHA02_001262"
     CDS             complement(8798..9517)
                     /locus_tag="ACHA02_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8669621.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9648..10799)
                     /locus_tag="ACHA02_001263"
     CDS             complement(9648..10799)
                     /locus_tag="ACHA02_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8669622.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11016..11756)
                     /locus_tag="ACHA02_001264"
     CDS             complement(11016..11756)
                     /locus_tag="ACHA02_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8669623.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11760..12083)
                     /gene="rsfS"
                     /locus_tag="ACHA02_001265"
     CDS             complement(11760..12083)
                     /gene="rsfS"
                     /locus_tag="ACHA02_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8669624.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12140..12733)
                     /gene="yqeK"
                     /locus_tag="ACHA02_001266"
     CDS             complement(12140..12733)
                     /gene="yqeK"
                     /locus_tag="ACHA02_001266"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8669625.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12717..13367)
                     /locus_tag="ACHA02_001267"
     CDS             complement(12717..13367)
                     /locus_tag="ACHA02_001267"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8669626.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13377..13688)
                     /gene="yhbY"
                     /locus_tag="ACHA02_001268"
     CDS             complement(13377..13688)
                     /gene="yhbY"
                     /locus_tag="ACHA02_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8669627.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13704..14813)
                     /gene="yqeH"
                     /locus_tag="ACHA02_001269"
     CDS             complement(13704..14813)
                     /gene="yqeH"
                     /locus_tag="ACHA02_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8669628.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14810..15337)
                     /locus_tag="ACHA02_001270"
     CDS             complement(14810..15337)
                     /locus_tag="ACHA02_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8669629.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15517..16302)
                     /locus_tag="ACHA02_001271"
     CDS             complement(15517..16302)
                     /locus_tag="ACHA02_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8669630.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16295..17164)
                     /gene="accD"
                     /locus_tag="ACHA02_001272"
     CDS             complement(16295..17164)
                     /gene="accD"
                     /locus_tag="ACHA02_001272"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8669631.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17165..18544)
                     /locus_tag="ACHA02_001273"
     CDS             complement(17165..18544)
                     /locus_tag="ACHA02_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8669632.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18552..18971)
                     /gene="fabZ"
                     /locus_tag="ACHA02_001274"
     CDS             complement(18552..18971)
                     /gene="fabZ"
                     /locus_tag="ACHA02_001274"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8669633.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(18996..19472)
                     /gene="accB"
                     /locus_tag="ACHA02_001275"
     CDS             complement(18996..19472)
                     /gene="accB"
                     /locus_tag="ACHA02_001275"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8669634.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19475..20710)
                     /gene="fabF"
                     /locus_tag="ACHA02_001276"
     CDS             complement(19475..20710)
                     /gene="fabF"
                     /locus_tag="ACHA02_001276"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8669635.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20729..21466)
                     /gene="fabG"
                     /locus_tag="ACHA02_001277"
     CDS             complement(20729..21466)
                     /gene="fabG"
                     /locus_tag="ACHA02_001277"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8669636.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21478..22395)
                     /gene="fabD"
                     /locus_tag="ACHA02_001278"
     CDS             complement(21478..22395)
                     /gene="fabD"
                     /locus_tag="ACHA02_001278"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8669637.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22502..22726)
                     /locus_tag="ACHA02_001279"
     CDS             complement(22502..22726)
                     /locus_tag="ACHA02_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8669638.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22779..23741)
                     /locus_tag="ACHA02_001280"
     CDS             complement(22779..23741)
                     /locus_tag="ACHA02_001280"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8669639.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23751..24191)
                     /locus_tag="ACHA02_001281"
     CDS             complement(23751..24191)
                     /locus_tag="ACHA02_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8669640.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24497..25462
                     /locus_tag="ACHA02_001282"
     CDS             24497..25462
                     /locus_tag="ACHA02_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8669641.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25519..25749)
                     /locus_tag="ACHA02_001283"
     CDS             complement(25519..25749)
                     /locus_tag="ACHA02_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8669642.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25835..26722)
                     /locus_tag="ACHA02_001284"
     CDS             complement(25835..26722)
                     /locus_tag="ACHA02_001284"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8669643.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26811..28034)
                     /locus_tag="ACHA02_001285"
     CDS             complement(26811..28034)
                     /locus_tag="ACHA02_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8669644.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28051..29097)
                     /locus_tag="ACHA02_001286"
     CDS             complement(28051..29097)
                     /locus_tag="ACHA02_001286"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8669645.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         8082 a         6401 c         5034 g         9651 t
ORIGIN      
        1 ccatatagaa ataaaaaatg atatcgatcg tagcaaattg ggccaaaagt gaattagttg
       61 ccgcacttcg attcgtgatt tcttttggtc tagatgtttg tacgagtaca tcggctatct
      121 tgctaagcgg gttatttcca aattgcgtca acgcaatgat ttcgatattc atgcttttcg
      181 ccagctcggc taatgcgact acatctgcac ttctacctga gtttgaaaca agccaaagca
      241 ctttcttttt ctcattgctg acaagttgcg ggagcaagac gtggatatct ttttcaaaaa
      301 tgattggctt accgacacga gaccattttt gcagaatatc ttctactact aaagaagagg
      361 cgccaacccc atataaaaaa acagtctctg ccgattccaa acactctacg actttttgga
      421 tcgtttcttc ttctagaata tctactgttt cttttatcgc tacttgtgca ttgttaccaa
      481 gtttgttttt gatagagtaa aaggactcat ttgcttctat atcactaaaa ccttcatcga
      541 ttccagtctg tagttctgtt gaaatcgtga ttttgaagtc cgtcagactt tgaaaaccga
      601 tttttttgga aaaacgtaca actgtcggcg cactcaaccc ggaagcttct gctaattcat
      661 tagctgtcat tgtagggacc ttctcaatat taccgataat gtagttggcc aacttttttt
      721 ctgctgaaga ataatcttcc atcatacttt caatacgact aattacgctc ataagttcct
      781 ccaccctttt aatgatagtc tatgtaagta taccatatca gacccttatt cacggtcgta
      841 tactggataa aaaacaaaaa aacaatccgc ccaacagaat atcttatccc atcaatccca
      901 cagattctat tgagcatctg tgtctttgag gaatacctgt tatctattgt tcggattgct
      961 ttctatctac tactttttta caggaaattt acctattagt tttctctgct gtaatttttg
     1021 acttctgctt ccatagcatc aataaagata ttcaaatctt ctacagctgt ttctttactt
     1081 ccataacgac gtacgttcac tgttgcatct tccaattcct tgtctccaac aactaattgg
     1141 taagggatct tttgtgtttg agaggcacgt atcttatagc ccattttttc attacgatca
     1201 tcaacttcta cacgcaaccc cttcatttgc aaacgttctt tgatttcata agcataatcg
     1261 ccatgtgcat caacagaaac agggatgatt gttgcttgta taggtgccaa ccatgttgga
     1321 aaagcgcctt tgtatacttc tgttaaataa gcgacaaaac gttccattgt agaaacaatt
     1381 ccgcggtgga taactactgg acgatgcgta ttttccccgt cttctccaac atatgtcaaa
     1441 tcaaagcgct ctggcaataa gaagtctaat tgtatagttg ataatgtttc ttctgtacca
     1501 agagctgttt tgacttgaac atctagtttt ggtccgtaaa aggctgcctc gccttctgct
     1561 tcaaagtaat ctagttctaa ttcatccata gcggctttca gcattgcttg ggctttttcc
     1621 cacattgcgt catcatcaaa atatttatct gtattgtttg gatcgcgata gctcaagcgg
     1681 aaacgataat ctgtaatatt gaagtcagca tatactgcca ccatcaattc aagcgtacgt
     1741 ttaaattcat ctttgatttg atcaggacga acaaatgtat gaccatcatt caacgtcatt
     1801 tctcgtacac gttgtaaacc tgacaaagca cctgattttt catagcgatg catcattcct
     1861 aattcagcga tacgaattgg taattcacga taagaatgga tcgtgttttt gtagaccatc
     1921 atgtgatgag ggcagttcat tgggcgaaga acaagcattt cgccatctcc catatccatt
     1981 ggcgggaaca tgtcttcttg ataatgatcc cagtgtccag atgttttgta caactcaaca
     2041 tctcccatta ttggtgtata tacatgttgg taacctagac taacttcttt gtctacgata
     2101 taacgttcga tcgtgcgtcg gattgttgcc ccttttggaa gccagaatgg caaaccagaa
     2161 cctacttctg gggaaaccat gaacaagtca agttcttttc ctagtttacg atggtctctt
     2221 tcttttgctt cttcacggag acggatatat tctttcaaag ctttcttgtc aaaaaaggct
     2281 gtcccgtata cacgttgcat catttgattg tttgagtttc cacgccaata agcacctgcc
     2341 actgataata atttaaatac ttgtatacgg ccagtggatg gaacatgtgg tccacgacac
     2401 aaatcgatga attcgccttg ttgataagca gtaatcactt cttcttctgg caattcacta
     2461 atcagttcta ctttgtacgg atcacttgca aaaatttcta atgcttcctg ttttgaaaga
     2521 acacggcgtt cgatcggcag attttctttg acgattttca tcatttctgc ttcgattgcc
     2581 ggcaaatctt ctgcagttac ttggttttgt ccattatctg tatcgtagta aaaccctgaa
     2641 tcgatagctg gtccaacgcc aaagtggata tttggataga ggcgacgcat ggcttgggcc
     2701 atcaaatgag ctgctgaatg acggactaag ccaagtgcat cttcatggtc tggtgtgatg
     2761 atctcaatac ttgcatcttc ctcgattgga cgagtcaaat caacgagttt tccgttcact
     2821 ttaccagcta aagctttttt tgctaagctt tttgaaatgc tttcagcaat tgccaatgtt
     2881 gttgttccag actcaaattc tttgactgca ccatctggaa aagtaatttt tatcataata
     2941 gtttcctcct tgaaatgttt tgattgatca gatcatcaag ctgaaaaaca aaaaaatcct
     3001 agcatctctt gaaaagagat actaggacga aaagttcgtg gttccaccta atgttcaaac
     3061 gcaaatgcgt tcctcaagcg ttgtaacggt ccgaccgccc ttgcttcaac aagggtttcg
     3121 aaagtggtca tcctttggtt ttctcctgga aaagtttcag cctagctttt cctctcttat
     3181 cgaagactct caaattcagt tggctttctc atcaatcaat tatgtgttta tttaaccact
     3241 aataaataga aaaatcaagt gggaaactaa acttattttt tccattcccc atttacaccg
     3301 tatgttcctt ttttcatctc attgatgatg acatggatgt gttcttttgg tgcctgcgtg
     3361 tttttatgaa cagcttccgt gatatctttc atcatatttt ctaattgttc cggactgcga
     3421 ccttctacta attcgacatg tacgaatggc ataatgctct ctcctctttc tgatcgttct
     3481 tttttcaact cgattctatc atactatgtt tgtcttgggt actgaatcag gaaaaatggc
     3541 tggatctttt tttgctctca aggcactcaa aatagagacc gtatccacga cttcttgcaa
     3601 aacagctccg aataatgcgg ggattagtcc cgtacttgca attaacatca aaaaaataca
     3661 aataaagatg ccaatcagta cagactgttt cgctattctc atcgtatctc ttgaaatcac
     3721 tactgctttt gctacacgca agagatcatc tttcaagatg accgcatctg cactttcact
     3781 agcagctgta gaaccatgag cccccattgc aataccgata tctgcagcag caagtgctgg
     3841 tgcgtcattc acgccgtctc ctaccatcgc aacaggtctt tgatctttag ggatactttc
     3901 taaaatggtc agtttatctt gaggcaagct acgggcatgt acagaatcta attggacttt
     3961 ttctgcaatc gtttgagcaa tggcagatcc atccccggtg atcatcatca aatttttcac
     4021 atcaagtttt cttagctcct tgattgtctt tgctgcttct gggcgaatct gatccgaaaa
     4081 taggatcgtt cctgcaaaag atcgatcaat tgttacatag attgctgtat tggacgtttc
     4141 tatttcttca gctgaaacaa aagaggcacg tccaatcttt acttcatgat tattgactac
     4201 tgcttggatt ccttgaccgg tcacttcttt cagttgacta acaggataca gttggctgtt
     4261 tttcacgttg ttcactaacg aacgagctaa gatatggcta gattcctgtt ctgcactagc
     4321 tgcatacaga agtagctctt ctttttttat tccattgact ggctgaatcg cctctacttc
     4381 caatatccct ttggtaagcg ttcctgtttt atcaaaagca atcgtttttg tacgtgcgag
     4441 cttttcaatc gttgttccag tttttacaac gatcccgttt ttacttgaac ggctcattcc
     4501 tgcaactaaa gcaatgggcg cagctagaat cagtggacaa ggtgatgcca ctaccagtac
     4561 ctcagcaaat cgtaaagggt cttttgagaa ccaccaagaa ataccaccga tcaaataagc
     4621 aatcaacgtg aaagggactg cataacgatc agctaaacgg acaaatcggg caggcttttc
     4681 ttttgattct tcgactagtt taataatcat ttgatactgg ctgtctgcgg cacgtttttc
     4741 agcgatcatt tggatcgatc cgtctccatt taaagaccct gacataatct gatctccagg
     4801 tttcttggtt tccgggcggg actcacctgt caaagaagat tcatcgaccg aagattctcc
     4861 tttggtcact cgaccatctg cgggtacaag ttcgccaggc ttgactagta actggtcatc
     4921 aatatttatt tcatcgatta ctatatcctg caattttcct tctttgaatc gatgggcaat
     4981 tcttggggtg ttatctaaca aagactgcag ttctttaccc gctcgtttat tcgcgtaatc
     5041 ctctagacta tctccgcccg taagcatgat caatatcatc aaactagccc agtattcttt
     5101 gacggctaat gttgcaagga ttgcggtaat cgctaataca tctacgccat atcttcctga
     5161 acgtaacgtc ttgatcattt ccaaaaacat caagaaggcg agaatccctc cagtaaatgc
     5221 gacaatcgca aatgcaaggg ccggctggtg aaaaacaaat tcacttaata aagctatccc
     5281 accaataagc aaagtaagta aaaatttttt gaaatgtgtc attgtttttt ctcctctttt
     5341 atgtgactta gagaggttag taataaattt ccagctgcct ctttcatctt aagcatagtc
     5401 cttgactagc actttttaaa acaataggcc ttaattgaaa acaactatca ctatcagcgt
     5461 ttccaaaatt catcagttct acaaacaaaa atattcagca aggattactt tttcgattca
     5521 tcagttaaaa agtacctttt ttcgaaaaac tatgctatac ttttaattcg ttatttacgg
     5581 cggaaggagc aaacatgcga tgaatgagaa acaactagaa caaacgcacg tggatgaaac
     5641 gatcgaacta atccagatcg aacaaaatat gctggataca aaacaacaag aattgactca
     5701 ggaaatgaat cattcgacaa aagacacagc taatcacaag attcgagctg gttccaatga
     5761 agctttttat gaatctgctg ttgaatatcg ccaacatgag caggaattgc ttttgaaata
     5821 ccatactctt gaagcacagc aaaaacgcct gcaaacgttg tctgtcatga aagatagtcc
     5881 ttattttgcc cgtatcgact ttaaagaaga ccgtgaaaca gaaacattat atctaggtat
     5941 cgcttcttta agagatctgg cagaagaaac aatcgttatt gactggcgcg caccaattgc
     6001 caatctttat tatgaaggtg agattggtcc cgcattttat gaaactgaaa cagatagaat
     6061 agatgtcgat ctgcagttga aacgacaatt taaaatccaa gaaggacgta ttttatctat
     6121 ggtagatacg tcggaagtaa tcaatgatga ctttttgcta gagatattgg atgaatcatc
     6181 aagcaaccat atgaaaaaca tcgtctctac gatacaaaaa gcacaaaatg caatcattcg
     6241 ggacacaaca agtaaagtaa tgctgattga aggaatcgcc ggaagtggaa agacatccgc
     6301 tttgcttcaa cgtattgctt ttctgttgta tcataatcgc aagtggctcg aggctgaaaa
     6361 cgttcttcta ttttcaccca accatttgtt ttctgattac atctcgactg tattgccttc
     6421 attaggtgaa agcggcgtgc ctacacagac attcaaaaat tatttggtgc agctgctgcc
     6481 agagttttct ttactagatg aacagcaaca agaaagtggt tttttgctag gggaaaaaga
     6541 tccgatacaa gtgatgaaaa gcggattgac cttagtcgat cagattgatc gctatatcca
     6601 aggcatcaca aactacggtc ccttgttccg tgatatgaaa ataaatggcc gtacgatcct
     6661 atcaaaggaa tcgattcgcc aatggtataa agaaacaaat gaacaattac ctttacatca
     6721 tagactttct ctattacaaa cgaagctcct gaaaaaatta ggcggtctgc aaaaagacga
     6781 aacacgtcag caatgggtca aagatctggc ggaagaacag ctccaagaac tatacgcaac
     6841 agatccaaat cttgaatata cagaacaaaa ggagcgttca ttacgtaaaa aactggccaa
     6901 ccaaatcgta cgtaaaagat tccgtcgaat ccatcgaggg atcatgaatt atcaatttgt
     6961 caatataacg aaacaatatc tccactttct acaaactgtg ccactttctc ttctaacacg
     7021 acataaaatc agcgagacca ggtggcagga acatttgaca actgttcgta cacacttgcg
     7081 tcaaagagaa ttgcgtcaag aggatgctgt actattcttt ttactgatgc gccgaacata
     7141 tccagtgacg gttacacaca aagctcgcta tatctttatt gatgaaatgc aggatttcgc
     7201 accatcacaa gtggcattgc tgcaagcctt gttcccaaaa gcaaatttga ctttctgtgg
     7261 agatctaaat caaaatgtct ttggaaatga aacgatcgtt ggatcattgg aagacctttt
     7321 cgtggaccgt gaagtcactc gtttccaatt aacgacaagc tatcgctcca caaaagaaat
     7381 cactgatttt gctaatcatt ttattgtgaa agaacatcaa gtagaaacaa cagcaagaaa
     7441 aggagacctt cctttgctca tccaaggaac aacagatgat atcaaattga attggttagc
     7501 agataccatt cgaacgactc agggaaaagc ccggtattgg cgtacagcaa tcatagctaa
     7561 aacacaagaa gaatgcgaag ctctttatgc tcaactgcca gacgagttga aacaacaagt
     7621 ccagctgatt ttaaatgaga gtgactttat gaagcgttcc attattttac ttccagcctt
     7681 tttagcaaaa ggtctggagt ttgaccgagt tttcttgtgg gatgtagatg cagaacattt
     7741 ttcttctcag caagacaaat tgatccttta caccatgtgt acacgtgcga tgcatgaatt
     7801 gacccttttt acctccaata atgaaagtcc tttgttaaac aatatggata tttcatctta
     7861 tgagaaaaaa gtactgtaag taaaaagaca actgctatca ttaatgttag cagttgtctt
     7921 tttatttaca ataataattg cagaaaaagt aattcaatca tttaaggtca tttattctat
     7981 atataaatcc aaatctgttt caggatattt taatttgaca agcgaaataa tcattttcag
     8041 ttcttccatg ttttctggat attctgggtc aaataccttg ctatgacggc caatcccgcc
     8101 attgtcgatc aatcggtaaa ttcgattcca ttcaatgaat tggtcaattg attggaaatt
     8161 gctgattgga tagccgccac aatccaaaat ctctgataca cgaaatgcta ggtatgcact
     8221 atcgatcact ttattttttt tcgtgtctat cttctcattt ctttcgataa gaattttttc
     8281 aggacgatgt ttcaagctcc actctaatag tagaaagaaa gcctccatcc gatactcttc
     8341 ttcaaataac cgaaaggctg aatcagaacg acgaaaaagc agcggaatca gataattttg
     8401 tctataaatc agcattggtg cgcggtccac catacactct aaataacggt tcagataccc
     8461 tgtaccgaca tagtttccta atcgatagcc ttcaacgccg aatcttgcgt atttttgcgc
     8521 taattttgaa caatcagtta cgttcacagc taactcttct actttgcggt aaccgcttaa
     8581 gatacgttca aaaggttcta aagtgatttt tgcttccata ttcttccccg tccatcttcc
     8641 acatacttca tatcatttca catgatactt atgctctcat cataccacag gttgaattag
     8701 aaaagaaaat aaatcacaca aaaaagcaag acaatctgtt agtgagattg tcttgctttt
     8761 cgaaaaagac tgaatacttt tcgttaaaac tggttgctta gtcttctaat gccacattat
     8821 ggaacacatg ctgtacatct tctagatctt ccagtgcatc aatcattttt tcaaatgttt
     8881 cttgatcttc tggagatagt gttacctcgt tttgagggat catttggatc tcagcgattg
     8941 aaaaatcagt gattcctttt tcttttaatg cttcttgtat actatggaaa tccgctactt
     9001 caccgtaaac gatgatttgt ccttcttctt ccattacatc gcgaacatcg atgtcttttt
     9061 ccattaggta ttcaaggatc tcgtcagcgt cttctcctgc aaaagcaaag attgctgtat
     9121 tgtcaaacat gtaagaaacc gctccggaaa cacccatgtt cccgccattt ttcccaaatg
     9181 ctgcccgtac gtctgctgct gttcgattca cattgttcgt caatgtatca acaatcacca
     9241 ttgagccgct tggtccaaat ccttcatagc gcaattcaga gtatgtttca tctcctgcac
     9301 cttttgcttt ttcgattgct cggtcaataa tatgttttgg tacattataa gttttagcgc
     9361 gatcaatgac gaaacgtagt ttttggtttg catgcggatc aggatcacct gattttgctg
     9421 ccgcgtagat ttcaatccca aattttgcat aaatccggct attattagca tctttagcag
     9481 ttttctttgc tacgatattt gcccatttac gtcccatatt ttcacttcgc tttctgattt
     9541 gatagtttca taacgcaaaa acgtcctgtt ccattatact tgtgaatcac gattctgtga
     9601 agcttttttt ctttcttcct tcattcttct catttttagt caattatcta tactcggatt
     9661 gggatttgcc cgaaattctg ttccggaata ttgcctccca attgatagat ttgatcactt
     9721 ctaactaaca gttccgcttc ttgtgcctct ttttttccga ttcgggaata aatagaaatc
     9781 tctttttttt cttttaagaa tcttcttcca gcatcggtaa atccaagtag tcgaagataa
     9841 tttttatttt gttggacagg aatctcttgc tttttgatat tcagcaagac ataacaagat
     9901 aatcgttgga tccgtgtcca tgtatatctt tttgttttta cctttgacac aaaatcctca
     9961 aaactatcag cagttgcagc tgctgttttc atgcgcgatt ctattccttc ggtcatctga
    10021 tagatatctg tcagttccag caaagtagaa gataacaacc gataacgcaa atacggccaa
    10081 taagattccc aagtctgcag cgaacaagtt gaaagatctt cttctgtctg aggcggcaca
    10141 agtccagcta ttctttttcc ttcttctaac gcatggcgaa tggctgttgc actggctatc
    10201 ctctgatctg tgatgatttt ttcatgatag cctgcacttt tacgtcggat cggatgtaat
    10261 tccatagggt ggacatactt ggcattttcc tttgcataac ttaatcccaa gatatgattt
    10321 ggcgaagaaa aatcaagcgt aatatcggga tacagactgc gaaaaacctc agtcattttt
    10381 tgcggataac tatacgtttg atcagacagt tcttgaaatg cctcttctat cctatcttga
    10441 ttctcttgga caaagcgtcc gaaactgctg taatcaaacg aagtctcttc ttctgttcca
    10501 aaacacaaac tgtcacagtc cagtgtttgc aagattttta ctgcaccttt agcaaaatag
    10561 tcagctgact gaaccgccca ttcgacggga agctccacta ctaaatccac accattttgt
    10621 aaagcttcat tcgcgcgatg ccatttatcg atgatagccg gttcgcctct ttgcaaaaaa
    10681 ttcccgctca ttacagcaat gacgacctct gctcctgtta tttttcgtgc catttccaca
    10741 tgatagcgat gtccattgtg aaaaggattg tactctacga taatcccgca gcttttcatg
    10801 cgcatcctcc ttcatagtta tcgataaaaa agaaatagag acaagtaaaa aaaggcaaaa
    10861 atcggaaaaa atacccgaac tatataaact attctcgcaa acacaatact tctgcgacga
    10921 aaagctgcac tgcaagagca atgcctatat cgttcggata tataatttat gttgaagatt
    10981 tattcttttc ttgttaactc gagcaggcaa aaccgctagt ctttgtatgc tacaaagaac
    11041 cagcgctttg atttctctga tgctgcttcg tctgtaaaat cagaaaatgc ttcgactttc
    11101 gaaaagccgc tgttttccag cattcttaaa taactttcca atggataggt acgctcttga
    11161 tgtagttcat cttcacgaat aaatcgacca gacccttctt gttcttcaac gaaaaaggta
    11221 agaaaatgtt cgatgctgtg tttctcgctt cctggataac tatcccacaa aaaagcaaaa
    11281 ttatcccctt gataatgata gctatattct ggaaaaacct catctacttg ataggtggag
    11341 tgtacgtcaa ataaaaatcg gccattttct tctaacgctt gatagacttc atcaaacact
    11401 tgctgtacct cttgtctatt tttcatatag cacagcgaat cagaaaaaca ggtaattgcc
    11461 tgatattgtc cgatttctga taaatcgagc atattgcctt ccacaaattg gatcgatgcg
    11521 tcttcttcat atgctcgctg gctggcaatc atcaacatct cttctgaaag gtctaaccct
    11581 gtcactttat atccatcttt tgcaaaggca accgctaatg ctccagtacc gcatgccagt
    11641 tccaaaatat tttttgtgtc ttcttgtaaa tgacgtttcg aaaaagctaa ccattgctga
    11701 taaagtgttt cgtccatcac ttcatcgtaa atgaaagcaa atgtttcata agccatatgt
    11761 taatccaacc attcactaag atcgactagc ggagcatctg accatagttt ttccagatta
    11821 tagaaatttc gttctggcgc atggaaaaca tgtacgatca catctccaag atcgatcaat
    11881 acccattttc cgccttcttt tccttcaata cgttttactt catatttgtt ttcttcttct
    11941 ttatcaatga tttcttctgt aattgcgttg atttgacgtt cgctatttgc tgaacagatc
    12001 atgaaataat ctgccaacaa ggaaacattt cgaacgtcta aagcgataat gtcttctgcc
    12061 cgttttgaat ctgctgcttc aatcgcaatt tttaattgtt ctttactatc tatgatgatt
    12121 tcctcctaat atgagcagct tatttttttg ctacccagta attatatgtt tcgattgttt
    12181 ttggataaat ctgttgttct tgcgcaagca aatgggccaa tgtatgtttt gtttcaaatg
    12241 ctacagcttc gtctagatca gcccatgcaa tggctcgtgc atcttgtacc ccaggaaaat
    12301 ctcgacctgg ttcaatatag tctgctacat aaatgatttt gtcaaggaga ctcatcttag
    12361 cagccccagt cgtatgcacc cggattgcat gaagaatctc tgcatcggtg attcctaatt
    12421 cccgctctac aaacgaagcg ccaacaagac catgccagat tgcattatta taattcaata
    12481 actccggatc aaaaccgtct cttacgatta ccattttgaa ctcctcatct ggtcgttctt
    12541 ttgcataatc atgtgtcaat gcagcaatac tagctttttc aggagaagca ccgtattttt
    12601 tggcaagagc cacggctgtt tcttctactc caagcacatg cttgaagcgt tgttcgctca
    12661 ttctcatctg tattttttgc atcaggtttt ctctagaata agcagtatat tcattactat
    12721 agtccatatt catacaaccc ttcattttga atataatcaa ttactttatc tggaacaagg
    12781 tagcggatag aacagccttc tttgatcttt tgacgtattt tcgtggaact gatgtcgatc
    12841 tcaggtacat ctacccaaat tacaggatat ggagtatccg ttgtgtatcc tggtcgacgt
    12901 atacccacaa agttgaccat agaagtcagc tcatcgattt tataccattt tggcaaatat
    12961 tctaccatat ccccaccgat aataaagtaa taatctgtat ccggattgtt ttgcgtcaat
    13021 tccttcatag tatcataggt gtaacttttc ccaccacgag ctaattcaat cgtttcaatc
    13081 tgcaaaaaag gattgtcttc caccgctaat tccaacatgt tgagtcgatg ttttgcatca
    13141 atcgtctgtt tctcatcaac atgaggtggc tgataagaag gcatcaaaaa tacgcggtca
    13201 agacctaaat tccggccagc ttgttcagcc atcactaaat gtgcaaggtg aacgggattg
    13261 aatgttccgc ccaagatccc tacttgcttt ttcttctcta agaattgagg catttcttca
    13321 ggaaataatt gtgaacgaac aaatgtctta gcttgcgtat tcatcatggt cactccttat
    13381 attgctttta cttcttttga tagcctttga tacttttctt ttgtggaagg tttgtataat
    13441 acaataacac gtccaatgat ttggacgatt tcgcaatcta tttctttttc taatacttga
    13501 gctgcttctt ctgcaatctc atctgtattt tgaagtaacg atactttgat cagttctctt
    13561 ttttctaaag cttcttcaat ttgaacgatc attgctgaat tgatcccacc ttttccgatt
    13621 tgaaaaatcg gctgaagatg atgcgctttg ctacgtaaaa aacgcttttg ttttcctctt
    13681 aaactcataa ttttccttct tccttaaatc aatgctttgc gtttcaatac atctacaccc
    13741 tttggcgccc agcccgcaat tactactggc gctgttacag tgatccagcc tagaccggca
    13801 aaaacgatgt ctgttttttc tttcacagaa aactcaaaac gaacaagctc cggaaatgcc
    13861 tcgacttcat caggacgagg aggctgtaat aatccaccta catgtttttg ataaaattcg
    13921 tctgcttttt ccagtttcgt tcgatgaata tttacatcat ttgacacata agcaataaat
    13981 gaagcacgat tgccgctgat aaaatcaaat cttgcaagtc cgcccaagaa aagtgtttgc
    14041 ccttcattca gctgataaac tttaggtttg atttctttta acggtgcaat gatccgcaaa
    14101 tcttttttac ctaagtagtg cgccatttga tggcgatgga taattcccgg tgtatcaatc
    14161 aaaaaatgcc cgtcatctaa aggaatctcg attttatcca aggtcgttcc tggaaactgc
    14221 gaagttgtga ttacatcttg tactcccgct gtatttttga tgatttgatt gataagcgtc
    14281 gattttccga catttgttac tccgacgata tagacatccc gtccatcgcg gtattcttcg
    14341 attttttcta atagttccgt catctcatga gatttcttag cactagttaa taatacatct
    14401 actggacgaa ttcctgcttc gtaagcacgt tccttcatcc attgtgttaa tttagttttt
    14461 ttcaatgatt taggtaatat atccactttg ttcccaacta aaagtactgg attgtcaccg
    14521 acaaaacggt gtagtcctgg gattagtgaa ccattgaagt caaaaatatc caccacattg
    14581 acgatcaatg catctttttg ccctaattca ttcagtaagc gcaaaaagtc gtcatccgtc
    14641 aatgagacat cttgggtctc attataatgg cgtaatctga aacaacgctg acaatagact
    14701 tccccggaag ctaaaccttt ttccaaagcc gtttgcggcg tgtatcctaa ttcaccgggt
    14761 ttgtttgttt gtataacggc accacagccg atacattgta attcttctgt cattcgattc
    14821 cacctcgcca gcccatatca gggtgttttg ctaatagata ggccataatt cttttttccc
    14881 aaaaacgatt aaattgtgtc ttccaactat ctgttgcgat aatgggtttt acaaggatgc
    14941 tacgtatccc tgcacgattg gctccacgta tatcagtcat aatctgatcg cctaccatta
    15001 ctatctcatc atctgctaaa tccaattgtt tctttgctat gttgatccct cttgctaaag
    15061 gtttcattgc tcgtgcaaca tacaaaagat caaatttttc aatcgctcgc gcgacacgtt
    15121 ttgaattatt gtttgaaaca acaacaacag ggattcctgc atttttcatt tctaaaatcc
    15181 aagtaagcaa ctcttctgtt ccatctggat tattccatgc aatcaacgta ttgtctaaat
    15241 cggtcaatac tgctttgatg cctagattct ttaattgggc aggtgtaatc tgatagatgg
    15301 cttctaccat ccatgttggt ttgtaatttg aaaacatgct gtctcctcat ctgcaaaaaa
    15361 tgaaggagca cataaagtac tcctcaaccc cgtattatac tgcatatcag caaaaaaaac
    15421 tacctctata ctcgttcgtc tttcagaaaa agcatttctt tacaaaatga ccaaaaataa
    15481 agattgatca cagaaaaatt tagaaacaaa aaacaactaa tattggcgga acctcttgta
    15541 tcgtttttcc agtaatttat gctgatctaa tcgaacaagt tccgtcaatc tttcaatcaa
    15601 cgcttttttc ataatgtgtg cgactttata tcgtggcaac tgctcgccgt tgaacgtttc
    15661 aggaataact cgatcaacga ttcctaactc gcgtagttct ggcgcagtaa ttttcatcaa
    15721 ctcagctgct tctgcggcgc gactaccgtc tttccacaaa atcgacgcaa atccttctgg
    15781 agaaagcaca gcataaatcg tttcttccag catccagact tcatccgcta aacctagagc
    15841 aagggcaccg ccacttccac cttcaccaat gatgatggcg atgatcggta cctttaaatc
    15901 agacatttcg agcagatttc tggcaatagc ctctccttcg ccgcgctctt ctgctcctac
    15961 accgcaatat gcacctgctg tattgatcaa ggtaatcacc ggtctattga acttttccgc
    16021 ttgtttcatc agtcgtaaag cttttcgata gccttcagga tggggagaac caaaatttct
    16081 gcggatattt tcctgcagat cccgtccttt ttgaataccg atcactgtta ccggtttgcc
    16141 atctaatcga gcgattccac caacaatcgc ttcgtcatct gcaaataaac gatcaccatg
    16201 gcattcctcg aaagaatcga agatctcttc aaaaaaatcc aagcttgtcc atcgttcttt
    16261 agctcttgct aactgaacga catcatgtgc tgtcttatcc acgatctatc catcctttct
    16321 gtgtatgcaa ttcaagaagc gtatggattt tctgtttcaa ttccatgcgg ggaacaatct
    16381 gatcgacaaa accatgagat aataagaatt cagccttttg gaaatcttct ggaagttcct
    16441 gcttgatcgt ctgttcgatg acgcgtcttc cagcaaaacc gatcaagctt tggggttctg
    16501 ctagaataat atcgccttcc attgcaaaac tggcagttac tcctccggtc gtcgggtccg
    16561 ttaaaacagt tagataaaaa agaccttcat tgctatgccg tttgacagca gcagagattt
    16621 ttgccatctg cattagagaa aagattcctt cttgcatccg tgcgccacct gaagctgtga
    16681 acaagacgac tggaagactt tcctttgttg ctcgttcgaa taatctggtg atcttttctc
    16741 cgacaatcgt tcccatactt cccatgataa aattaggatc catcactcca ataatgaagg
    16801 gaatcccctg aatggttgct ttaccagtga ggactgcttc atgaagtcct gttttatctt
    16861 gcattttttt aattttttcc gtatattcgg gaaatgctag cggatctttg gttacaagat
    16921 ccgtatccca ttcttcaaaa cttttttcat caataatcaa agctagacgc tgccatgcac
    16981 cgattcgaaa agcgtatccg caatgagggc atactttttc ttcaccgatg tctttcgtat
    17041 aaaggatatg tttgcaattt ggacactttg cccacatgtt atcaggaaca gatggcgagt
    17101 tagcaggttt gctctctcta ttaggattga tccgaatata tttctttttc ttaaacagag
    17161 ccatctatgc gcctcctatc tccgtttctg gtgtccaatt cggtaaaaat tcttcttgta
    17221 aaaatgctgt gctgtaatca cctgctatga ctgcaggatg actaatcaaa tccatctgaa
    17281 attctgcgtt cgtgatgatt ccttctgtga taaattcact taaggctcgc tgcatcttca
    17341 ttaatgcttc aaaacgcgta tttccatgga cgattatttt tgcaatcatt gaatcataat
    17401 atggcgggat cgtatatccc gggtaaactg cactgtctac tcgcagtccc atcccacctg
    17461 ctggaagcag taagttttgg atcttccctg gagaaggagc aaaatggaaa gcaggatttt
    17521 ctgcattaat ccggcattcg attgcgtgtc cttggaatac tacttcttct tgacacacat
    17581 tcaaaggctc accggctgct atctccacct gtttttttac taaatcgatt cctgtgacca
    17641 tttctgttac tggatgttcc acttggattc gagtattcat ttccatgaaa taaaaatcgc
    17701 cttcttcatc catcagaaac tcgatcgttc cggcattttc ataattgact gctttagctg
    17761 ctcgaacagc agcgtctcct agtgcttctc tttttgtttg tgagatcact acagaaggag
    17821 attcttccag tactttttga ttgttgcgtt gtaaagaaca atctcgctcg cctaaatgaa
    17881 tgacatgacc aaatcgatca cctaagatct gaacttctat atggcgagca ggataaatga
    17941 ttttttccaa atacatatca tcatttccaa atgccgctgc tgcttcttgc tgagctgaag
    18001 aaaaatgctg tgggagctct tcttttgatt gaactttccg aatgcctttc ccgccaccac
    18061 ctgcagctgc tttcagcatc actggataac caattctgtc tgctatttcc aatgcttctt
    18121 ctactgtaga taatacacca gtacttcctg gaatgactgg gacatttgct tcttgcatca
    18181 attgacgcgc attgatctta tttcccattg catcgatcgt tgcactttta ggaccaataa
    18241 atgtgatatt gcactcttca cacatggagg caaatcggct attttcagat aaaaaaccaa
    18301 atcctggatg aattgcttcg gcttttgtca cgattgcagc gctcagtact tcctgaacat
    18361 tcagatatga atcaacggct tttgccgggc caatgcagat tgcttcatct gctaactgtg
    18421 tatgaagtgc ttcggcatct gcttctgaat atatcgctac ggtttggatc cctagttcgc
    18481 ggcatgcacg aatgatacgt acagcgattt caccgcggtt agctatcaat actttagaaa
    18541 acatatttca cctatccaat cataaaggtt aattccgctt cggctacttt tttaccgtct
    18601 actttagcga ttcctttgcc tattccggct gaagctttca ctttcaatat ctcaacttca
    18661 aggtataagg tatctcccgg tgtaactttc ttacggaatt ttgctttgtc caatccgccg
    18721 aaataagccg tctttccctt gaattgttca agagaaagta atgcaactgc tcctgcttga
    18781 gccattgctt caacaatcaa tacccctggc ataacaggtt cttctggaaa atgtccttga
    18841 aaaaagggct cattgatcgt tacatttttc ttagcaacga tccgttctcc ttcaaccatt
    18901 tcttccactc gatcaatcaa aagcattgga taacgatgtg gaatgatttc ttttatttct
    18961 tgtatgttca ttgttttccc tctttcctgc tttgcttatt tttgctgaat cctaaagagt
    19021 ggctgtccat attcgacaac tgactcgttt tctacaagga tttctgtgat cactccgtcg
    19081 gcctcactag ttatctcatt catcagtttc atcgcttcaa taatgcagat aacttctcct
    19141 ttggcaactt gatcacctac ttttttgaaa gctggtttgt cgggcgctgg ttgcagataa
    19201 acgattccca cgattggcga aacaacctcg attccatcaa atactgccga ttctttttct
    19261 ttcattattt ctggagcagt tgttgcttgt tcaggtatga cattgactgg ctccgaaact
    19321 ggcaacgttt cgtttgtttg aaccatattc atgcctcgac ttgttttatt tttattcata
    19381 tacaattcaa attgaccttc acgcaaatcg aattccgtta gcgaggattg atcaaattga
    19441 ctgactaatt ctctgatttc attgatatcc aaaattattc ctcccagcgt ttcatgcaca
    19501 gaaccgcatt atgtccgcca aaccctaatg aattactcat tgcataagta aaacttgcag
    19561 gtttgctttc gtttttgaca actgtcaagt caatctcagg atcttgtttt tctacattga
    19621 tcgtaggtgg gataaattga tgttctaatg ccagcaaagt tgccaaggct tcgatcccgc
    19681 cggctgctcc caataaatgt ccagtcatcg atttcgtgct gctgacgtaa gtatctctga
    19741 aattttcacc taatgcgatt tggattgctt ttgcttctgc ctggtcattg gctggtgtac
    19801 ttgttccgtg agcattgata tatccgactt gttccggctg tacaccagct tcttccatcg
    19861 caagcttcat tgctttagat gcaccagaac catctggagt aggagctgtc atatggtaag
    19921 cgtcacagtt tgcaccgtat ccaacgattt ctccatagat tttggcaccg cgagcttgtg
    19981 catgttctaa agattcaaga actagaacgc ctgctccttc acccatcaca aaaccattcc
    20041 gattttcgtc aaacggaatc gaaccacgat taggatcagt tgattctgag agtgctgtta
    20101 aagctgcaaa tccagcaatc cccatctcac aaatcgttgc ttctgttcct ccagcaagaa
    20161 tgacatcaga atagccatgt ttgatgttac ggaatgcttc cccaattgaa ttcgttccgc
    20221 tagcacaagc tgttacgata gctgtacaaa taccttttgc acctacacgc aaagcaatat
    20281 ttccagctgc catgttggca atcgccattg gtacgaacat tggagaaact cgttttgcac
    20341 ctttttcatg tagacgaata acttggtttt cgattgttgg aagaccaccg atcccagatc
    20401 cgatgatgac gccaaaccgg tcttgattca ttttttcttt atctaaaccg ctcatctcca
    20461 ttgcttctaa agcagcgtag attccataga tcgaaaacat atccattcgt ttgctatctt
    20521 ttttaataaa atatttatcg taagggaaat ctttgacttc acccgctaag gtaatccctg
    20581 tttctgaagc atcaaattta gtgatcggtg cgatgccatt cgtcccctct tttaagctat
    20641 tcaaaaagct gtctgcgtca tttccaatag gtgaagtgac tccatagcca gtaattacca
    20701 ctcgattcat ttgtttgctc tccttttctt atccgtgcat gaccaatccg ccatcgacat
    20761 tgatgacttg tccggtgata tatggatttt gtgctaagaa aactgctgtt tgtgcaatat
    20821 cttctacttg tccgaaacgc tgcatcggaa tttgcttttc cgcttgttcc ttgactttgt
    20881 ctgctaaaac ttccgtcata tcagttgtga tgaatcctgg cgcaatggca ttacatgtaa
    20941 tgcctctagt tgcagcttct cgtgccacag attttgttaa tcctactacg ccagctttac
    21001 tagctgcata atttgcttga ccaatatttc caatcaaccc agatacacta gagagattaa
    21061 tgatggcccc ttctcgctgc ttcatcattt ttttcaatac gtgctgcgtc atattgaagg
    21121 ttccaatcaa attgatatcc agacattttt tgaaatcttc tgcatccatt cgcatcacaa
    21181 gtttgtcatt tgtgattccg gcattgttta ctaacacatg gatcgatcca agcttttctt
    21241 ctgcttcttt gatcatttgt cccgctttct cataatcaga gatgtcaccg gaaactccta
    21301 cgcattttac accgaatgct tcgatttctt ctatcttttc ttttggaatc tctccgcgac
    21361 cattcaaaat gatatttgct ccagcttttg caaaagctaa ggccatcgct ttgccgattc
    21421 ctcttgtact acctgtgata aatacatttt ttccttttac atccataacg ttcctcctta
    21481 tagtattgct attgtttcat caaatgtctg cttattctca acacgcagaa ctggtattgt
    21541 tttatcgatt tttttcataa atccgcttaa tacttttccg gggccgactt cgatgacttg
    21601 ttcgatgccg attgttttta acttatggat actctcatag aaacgaacag gttgcatgac
    21661 ttgctgttca agtagtcccg cgattgtttc tttcttcatt atttccgttg ttgtgttgct
    21721 tatgatagga aaagaaggtt cagagaattg aatctgactt aggtccttcg ccagtttttt
    21781 tgctgccggt tctaaaatag ctgtatgaaa aggcccgctg acatttagag ggatcattct
    21841 tttgaaccct ttttctttta atagtaaaac agcttcgtct actgcttttt cttcgccacc
    21901 aatcacgatc tgctgaggtg tattataatt tgcaggagat acgatgccgt acttgctagc
    21961 ttcatgacag ctttcctcga tcgtttcaat cggcgcattc atcactgcaa ccattttccc
    22021 gcttccagct ggggcagctt ctgtcatata agcgcctctt tttgctacta acgcaactgc
    22081 ttcagtgaag gatagcgcat tactagctac taatgccgaa tattctccta aactcagtcc
    22141 taaagctgca tctgggatga ttccatgctc cttcaataag cgataatacg cgatgctgac
    22201 tgttagaata gctggctgtg tatattgcgt ctgatccaaa cgttcatttt ctgtaaagca
    22261 aagttcagcc atatcgtagc cgagaatctc acttgcttca tcaaatgtct gctttacaac
    22321 ggcttcttca tataaatctt ttcccatacc ttgatattgt gcaccttggc cactaaataa
    22381 gaatgctgtt ttcatgtttt tctccaatat atccagaata tatgtttgat tcttcctatt
    22441 caaaaaggcc gagacattcc tatttcagga aatgtcccag cccccaacct aattttaatg
    22501 attatgcagt ttgtttttct acgtaagtca ctaaatcttg aaccgttttg attccgtctt
    22561 ctgtttcgat tttcacgtca aattcatctt caatttcatt gatgatttgg aataaatcca
    22621 agctgtcagc ttctaagtct tcttggatgt cagtagtcaa tttgatttct tcagtttctt
    22681 ttcctaattc ttctgcgatg atttcttgaa ttttttcgaa taccataata atatattcct
    22741 ccgatattgt ttgcttttta tttttataat ttaacagtct acaactccat gagaatactt
    22801 ccccatgtta aacctccgcc atagcctgta aatattactt tctgctgttt tcctaaggtg
    22861 atctttccac tagaaactgc ttcatccaat aagataggga tgcttgcagc agatgtattc
    22921 ccatatttat ccatattggt caggaacttt tcttgcggca tctttacttt tcgggcaatt
    22981 ttctcaataa ttctgaggtt tgcctgatgc aaaagcagat aatcaactgg tttgtctttc
    23041 atgacttctt ggatattgtt tgcaacgtct cgtacggcaa aatcaaaaat atctctgcca
    23101 gtcatttgca aataatacga accttctgaa tcttctgaat agaacggatt tttattttgc
    23161 tggtagccag cggtcaatga gctacttctg gtaccgtcag ctgctaattt ttctttcagc
    23221 aattttttct gtggattcgc ttctaagaga atcccacctg ctccatcgcc aaacaacaca
    23281 gcagtcgtac gatcagacca atcgattagc ttagatagtg tttcaccacc aatcaccagt
    23341 cccttagttg agccgcagcg gatcattttt tcagccatgc ttaacgcata aacaaatcct
    23401 gagcacgcag cactgatatc aaaagcgata gcatgagttg ctcccaattt cccttgtact
    23461 tgtgctgcta cagatggtgt cgtgtaatcg ggcgacattg ttgctactaa aataaaatcc
    23521 aaatctttag ggtttagatt tttctgtttt atcaattttt ccgccactaa aatgcataaa
    23581 tcagaagtct cctgatttgt tacacatctt cgttcacgaa tacccgtcct tgacgaaatc
    23641 cattcatcag aggtatccat catcttggaa agatcattat tagtaataat cttttcagga
    23701 aggacgctgg ctgttgcagt aatcattcca aattgttcca ttaaatatcc tcacttatag
    23761 tcttgcaaga agtcgtgcaa gtttttcagt gcatccagca aggcatgctg ttcctccgta
    23821 gccattccgt ctaagatgtt cttgaccatt tcacgatgaa aatgctgatg gacccgaaat
    23881 agtaactttc cttttttcgt tagtcccaac tttacaacgc ggcggtcatc ttcactgcgg
    23941 atacgttcga catagccttt ttttactaac ttgttaatag cagtggtcaa ggtcccaaca
    24001 gtaattgata gttctttggc tacttctgaa gttgtttttt tcttgtacat cccgattgcc
    24061 tcgatcgtgt gcatctctgt gattgacaga tctttgaatt gagatttttt cagctcagac
    24121 tcttcgattg tcaaaatatc attgaagaca ctaacaagat aatcatttac tgtttttaaa
    24181 tctggttcca tgttccaccg tccactaatt agtttgatta tcaaatcttt tgatgttcaa
    24241 actatatctt aacttttttc aaaatgcaac cttaaaagat cattttttta ctatatgagc
    24301 tttttttaag attttactca tttaaaacaa ctgataaatc agcatttata atttctttta
    24361 ttagaaaaat catctatctc acatattttg attatcaaag tattttctgt gaacagaact
    24421 atagaattcc ctaaaacttg tccattattt caaaatttgt tatagtcttt ttattcaaag
    24481 cgtaaaagga gatcctatgt taaaagaaaa aatgaaaact ctccccatgc tgccgggtgt
    24541 ctatcttatg aaagaccgag atggtaaggt catctatgtt ggtaaagcaa aacggctcaa
    24601 aaatagagta agcagctatt ttcatcagaa taaacagcat tcaaaaaaag tattacgtat
    24661 gattcatcat ataacagatt ttgatttcgt cgtagtagac acggaattag atgctttatt
    24721 gctcgagtgt caattgatcc aacattaccg tcctttttat aaccgacaaa tgaattattt
    24781 cagtaattat aattatgtac atattaccaa taagggattt gtattaacag atactcctac
    24841 ggctcgaaca tacgggcctt ttcgtctcta taaaaaaatg ccgtctattt tgcgtataat
    24901 ggaagaaact tatcaaatgc cttggttgtc tgaaattagt ttgttagctt tgcgagtcca
    24961 gcttcctgac ctacaggaaa tgacttttga acagaaaaaa aaggaactcc aagggctgtt
    25021 tcaaggccga aataaaaagc tactgactta cttgaaaaaa agacaacaac atttcatcta
    25081 tcagttgaat ttcgaaaaag ccggtatgct tcaaaaagat atcgaattag ttacttattt
    25141 tattagacgg atccaggaac aaaagcagtt tttaagaaca ccatcgctca ccttcagcat
    25201 gccattagca gcagatgaga gtcagaaaaa gcactacctc gtttgttatg gtcaacttgc
    25261 agaaacaatg attacctctg gtgacgtttc tcctgacttc tattatgaaa agaaagaagc
    25321 tcatttatct ttgaaacgtc aattgtcaaa agaagaaatc gatcctgtac aaatcttgat
    25381 cagttaccgc aaaaaattag aaaaagaaca gtttgaaatt gaactgttaa acaaaaaaga
    25441 agctgaaaag caactaaatt agttgcttct cggcttcttt taggcatctt caacttcaaa
    25501 tgtcaattat cctaaaattt attggattaa gtcatagatt tccatcgcta cgatatcgat
    25561 attatcaaat tcgtattgct gatgagtatc tgtttcagtt aattcaaatg tatcggtcgc
    25621 tttatcataa gttactgtac acttctctac gccttctttt tcaaaacggc gaacttgcag
    25681 ttcattatct gctgactcga ccatagcctc cagtcgtttt atgattgcga ctaattgtga
    25741 atgtgtcatc ggaggtcctc cttttcttat ttctgttata ttgtatcaaa agcatagcct
    25801 acttgcattg attttctcta aaaaaactcg ttttttatga ttttttttct ggtatttttc
    25861 cttcaggcca ccaaagtttg atcaaagctt gggttccgtc attctcaaaa cctttatctg
    25921 ccaactcttc atatagtttc aatgcttggg tagttgctgg caatacaaga tccatttttt
    25981 ctgcttcttc taatgcaatc tttaaatctt tgataaaatg tttgacaaaa aaaccaggtg
    26041 tgtaatcttc ttttaaaata cgaggagaat aattgcttaa agaccagtta gctgcacttc
    26101 cacctgaaag cgtctcaatc actttcttta aatcaagtcc gtttttttgt gcatagacaa
    26161 gcatttccgt cattcctgtc atcgtacctg caatcatgat ctgattagcc atttttgtat
    26221 gctgaccttt cccagcagaa ccatgcaatg taaatgtcgt accaaattct ttgaataaag
    26281 gcagtacttg atcataaacg gcttcttctc caccgaccat gatcgtcagt gtaccattct
    26341 ttgctcctaa gtcaccaccg gataccggtg catctaacgc atctgcgcct ttttcttttg
    26401 ctgtttttgc aattttttct gccaggcttg gtgtacttgt agtcaaatca actaaaatat
    26461 gtcctgagat atctgcagaa aaaatccccg actgtccata gtagatttct tctacatctt
    26521 tggggtaacc taccattgta aagagaatat tactttgttc tgctaccgct tttggtgtat
    26581 ctgcccagat agctccttct gttactaaag gatcagcttt tgcttttgtc cgattataga
    26641 cagctacttc gtgtcctgct tctagtagat ggcgtgcgac agcacttccc atgacgcctg
    26701 ttccgataaa accaagtttc attttttcga cctcctaaaa atcaaaaatc cttgcttcta
    26761 tgctagtata cgctagaaac aaggattttt tcataaaaaa tgtttgaatt ttatctttta
    26821 aatgaattta attttatttt gattcgttca atcgagtctt cttcttcttt tcttcgcatc
    26881 tcttcttcat gataaacaac catgtcttga taaaattcga tcatagcttc gccgaaatgt
    26941 gtagatgaaa tatcataaag tttagcttct aaaacttttg gatctttagg aacttcactg
    27001 gaaatataat caataagtgc tggtgcaaag tcttcgtcgg tttcaaaagt tttccctaat
    27061 gaaggatgat cgaacaatcg gttcaaataa gcatttcctt ccacgacaca aggtgttccg
    27121 gctgccatcg cttctgtata agtaagccct tgtgtttctg aagtagaagc actgacaaaa
    27181 taatctgccg ctttataata aagtgctaca tcattgttgt ccacttctcc tgtgaactgc
    27241 acaaattctt ctaactgcag ttctgagatc aattcctcta actcttctac ataaggtcct
    27301 tttcctacga taactaatct ggcgttagga taagcggaga tgacctgagg aaacccatta
    27361 atcaaggctt ggatattttt ctcataagaa atcctgctta aagaaagaat catcaaatgg
    27421 ttttcctgaa gacctagtga ctcccttaat ttgctgatat cctgctgagt gatatcagaa
    27481 cgtttgaatt tttcaatgtc gataccagta ggaatcacgc gaagcggtgc tattacacca
    27541 tagctgcgta atgtatcaat aacacgttca cttggacaaa ctacacctgt cgtatgattt
    27601 gcaaataatc tagacaagta ttttacatgg gattgcctga tcacttttcc tttaccgata
    27661 taatgaaggt agtcttcata catcgtatgg taagtatgga tcagaggtat tttcaacttc
    27721 ttagctacca tcttcccaag tatccctgct ccgaattctg tatgggtatg aataagatct
    27781 agttctagtt ctttggcaac caaataagca taccacatgc ctctaacaac aactcttcga
    27841 tccttgaaag aaataaatgg tacacttggc attcgaatca catctttttc caacttatcc
    27901 gcattaggat cggtcgttgt aaaaatatag acttcatgcc catgtctttc taattcttgt
    27961 tttaatgttt tgatcgatgt agcaactccg ctgacttgcg gaaagtaggt atcggtaaaa
    28021 aagccgattt tcatgttttt cgcctccaaa ctagtccttg acctgattta ctttttttaa
    28081 tttgctagag actttttcat tcagtacact ttcgtataca tttttaagtt cttcaccgat
    28141 tttattgata cttcgctctt ttgctgtttg ataaccggct tgtgtcgtac tcggtagttc
    28201 ttgattggta atctgttgga tcagtcggat aaattcttcg tttgtcttcc ccatatagca
    28261 gttcttgcta tcttctaacc agccattata cactggaata tctcgaacaa gtacgttttg
    28321 tttgcttgct aaagcttcta aaacaacgat cccttctgtc tcttcataag aagggaaaaa
    28381 gaatagatct gcacctgaat acgcaccttc tatgatctct ccacgaatgt aaccaggaaa
    28441 caggacattt tcaggatgat cttcttttac aattttccgg atgttccgag gaatcgagta
    28501 catgggaaca tgaccaaacc aaataaatgt gtattcgggc atcttcttag caatttctac
    28561 gaaatcaatg attcctttac gttcgaaaaa caaaccgaca caaatgatta ctttttgatc
    28621 cggagacagt ttgaaatatt ctctgaactt ttgttctttc attggatcag ggtgatattt
    28681 ctctaagtcg atcccattgg aaatagcttg aatcggcaca cggataccat agctttccaa
    28741 caactgtttt gaataaggtg ttggcgtgat cagatagtcc gctttttggt acaatcctac
    28801 cagatatttt tttacgatag gagaaagctg gttcgatccg ataaaggaat ttcggaaatc
    28861 ttcctctgta gaatgcgcat gatagacaac tttttttcct tctcttctgg ctttattgac
    28921 catattatga ctattgattc cgtatgtatt gatgtgcagg atgtcataat cttctttttc
    28981 atctagtgta taagagatac cgacttccgt cagagcacgt ttttgatgat caagcgcacg
    29041 cccgattcca gattttgcaa gcatcttttc actttcaaaa tataataata ccttcacctt
    29101 acgtccccct tcacttatat agtataacat agcctacaaa ctgtcactca aacttaagcc
    29161 ctattttt
//
LOCUS       DAYYDS010000030        28291 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000030 DAYYDS010000000
VERSION     DAYYDS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.030"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..138)
                     /locus_tag="ACHA02_001287"
                     /pseudo
     CDS             complement(13..138)
                     /locus_tag="ACHA02_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
     gene            complement(131..1222)
                     /gene="phnW"
                     /locus_tag="ACHA02_001288"
     CDS             complement(131..1222)
                     /gene="phnW"
                     /locus_tag="ACHA02_001288"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM8669646.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            complement(1439..1918)
                     /locus_tag="ACHA02_001289"
     CDS             complement(1439..1918)
                     /locus_tag="ACHA02_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8669647.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            2277..3059
                     /gene="rpsB"
                     /locus_tag="ACHA02_001290"
     CDS             2277..3059
                     /gene="rpsB"
                     /locus_tag="ACHA02_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8669648.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3158..4039
                     /gene="tsf"
                     /locus_tag="ACHA02_001291"
     CDS             3158..4039
                     /gene="tsf"
                     /locus_tag="ACHA02_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8669649.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            4175..4897
                     /gene="pyrH"
                     /locus_tag="ACHA02_001292"
     CDS             4175..4897
                     /gene="pyrH"
                     /locus_tag="ACHA02_001292"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8669650.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            4900..5457
                     /gene="frr"
                     /locus_tag="ACHA02_001293"
     CDS             4900..5457
                     /gene="frr"
                     /locus_tag="ACHA02_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8669651.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            5652..6464
                     /locus_tag="ACHA02_001294"
     CDS             5652..6464
                     /locus_tag="ACHA02_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8669652.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            6461..7261
                     /locus_tag="ACHA02_001295"
     CDS             6461..7261
                     /locus_tag="ACHA02_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8669653.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            7422..8690
                     /gene="rseP"
                     /locus_tag="ACHA02_001296"
     CDS             7422..8690
                     /gene="rseP"
                     /locus_tag="ACHA02_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8669654.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            8758..10467
                     /locus_tag="ACHA02_001297"
     CDS             8758..10467
                     /locus_tag="ACHA02_001297"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8669655.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            10679..15037
                     /locus_tag="ACHA02_001298"
     CDS             10679..15037
                     /locus_tag="ACHA02_001298"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8669656.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            15182..15655
                     /gene="rimP"
                     /locus_tag="ACHA02_001299"
     CDS             15182..15655
                     /gene="rimP"
                     /locus_tag="ACHA02_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8669657.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            15678..16853
                     /gene="nusA"
                     /locus_tag="ACHA02_001300"
     CDS             15678..16853
                     /gene="nusA"
                     /locus_tag="ACHA02_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8669658.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            16875..17168
                     /locus_tag="ACHA02_001301"
     CDS             16875..17168
                     /locus_tag="ACHA02_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8669659.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            17165..17476
                     /locus_tag="ACHA02_001302"
     CDS             17165..17476
                     /locus_tag="ACHA02_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8669660.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            17489..19795
                     /gene="infB"
                     /locus_tag="ACHA02_001303"
     CDS             17489..19795
                     /gene="infB"
                     /locus_tag="ACHA02_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8669661.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            19822..20169
                     /gene="rbfA"
                     /locus_tag="ACHA02_001304"
     CDS             19822..20169
                     /gene="rbfA"
                     /locus_tag="ACHA02_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8669662.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(20326..20782)
                     /locus_tag="ACHA02_001305"
                     /pseudo
     CDS             complement(20326..20782)
                     /locus_tag="ACHA02_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            20947..23309
                     /locus_tag="ACHA02_001306"
                     /pseudo
     CDS             20947..23309
                     /locus_tag="ACHA02_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            23677..24600
                     /gene="truB"
                     /locus_tag="ACHA02_001307"
     CDS             23677..24600
                     /gene="truB"
                     /locus_tag="ACHA02_001307"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8669663.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            24604..25545
                     /gene="ribF"
                     /locus_tag="ACHA02_001308"
     CDS             24604..25545
                     /gene="ribF"
                     /locus_tag="ACHA02_001308"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8669664.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            25897..26682
                     /locus_tag="ACHA02_001309"
     CDS             25897..26682
                     /locus_tag="ACHA02_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8669665.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            26695..27546
                     /locus_tag="ACHA02_001310"
     CDS             26695..27546
                     /locus_tag="ACHA02_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8669666.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(27670..28122)
                     /locus_tag="ACHA02_001311"
     CDS             complement(27670..28122)
                     /locus_tag="ACHA02_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8669667.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         9625 a         4978 c         6105 g         7583 t
ORIGIN      
        1 tcccatcggt tagcggattt ctttgtcagt cagttggatt cctttttcta gaaaagcatt
       61 tctaaaagcg tggacaggag ccatacagcc aaagtccaca gttgttcctg cccaatcaaa
      121 aattacagtt ttaatcatta acgttctcct tatagtcttt gataattgct gtcagttcca
      181 aaatatcttc cggatagatc tcgccaatat ttccgatacg aaaacattca tgatcgctga
      241 ttttcccggg atagatcaca aatccatttt gctttaaata ctgataaaaa tgattaaaat
      301 caaatcgcca gtccttaggg taaagaaaag aggtgatgaa tggcccctga tattcttgaa
      361 tgaaaggctg gaaccccatc tctgtcattt tttgtcgtag cacatgatta ttggcgagat
      421 aacgtgaata tcgagtttcg attcctcctt ctgcctctag ttccttcagc gcttgttgaa
      481 aagccaaaac cgtatgggta ggggaagtga aacgccattt gccattctcg ttttccatca
      541 cgcggtactg gtcatataga tcaagtgaca aacttcggct aacatttttt cgtttttcca
      601 gttccgcttt attagcaagt acaaaagaaa agcctggaac accttgaata catttgtttg
      661 agctggaaat caaaaaatca atccctagtt cggctacaca aatcggtatc ccaccaaagc
      721 ttgacatcgc gtccactata aacaaaagat ctctttcttg tgtgacagca gcaatctctt
      781 ccaaattatt caaaataccg gaagtagtct cgctatggac cattgctagt gcggagatag
      841 acggattctc ttcaatcagc tgtgtaatca catgagcttg gaggatttga tgttccgata
      901 cctcataaac ggtatagttc agtttcagac attcgcacat gttgatcatt cgttgtctat
      961 atgcgccgtt tgaacaaatc agtacagtat cttctggcct gatcacagaa cttaataccg
     1021 attctacccc gaaggagctg ctaccttgca ttaagaccgt tgtgtatatt ccttccgata
     1081 cttgagcaag atccaataat ttttttctta tccattgtgt aatttctttg tattcttgat
     1141 cccaagtaca atgatcgatc agcatagcat ttttcactgt ttcagaagtc gtcaatggtc
     1201 cagaagttaa taatttatac atatgatttc ctttcatttc ttttttgaaa tacacaaaga
     1261 gcaagaagct gctcttgtta acatccaata taaatgatga ttattgtgat tcaatcaagg
     1321 tgttgaaaaa tttgaataaa aaatgatgta tttttgtaaa gagtgtaaag gtttattcaa
     1381 taaggaaaat attgaaaata aaaaaacacg cattccatat taggaatacg tgctctgatt
     1441 aaaaaagtga tacttcagtt ggatggtgaa gaaattcttc tactcgtttt gcgtcctcct
     1501 cattttccga gatgaccatg atcgtatcga atccagcaat tgtactgaca atagagggag
     1561 gtttgatctc atctaaaaca gatgcaaata aatgagcatt gtctggcaag gtattgacga
     1621 tcgtcaaaaa gtggactcgt tcgactttca atacaatgtc ttcaatcatt tgaaccagcc
     1681 gatcctcttc ctcagtccta gtctcaggag ttcctttttc gcgaaacaat acgaagcggg
     1741 aaacgccatt ttcatcaggt gccttcacga tttttagatc acggatatct cgagaaatcg
     1801 ttgcctgcgt ggcggtaacg ccataatgcc caagtttgtt taatagttct tcttgcgtac
     1861 ggatcgggaa ttcttcaatg acttgtttaa tcagcaaatg tctttgagct ttgcgcattt
     1921 tctttctcct ttacgaaaga tttcacatgt atcataacaa aaaaaagaag gattgcccaa
     1981 taatattcgt cttttatacc taaaattatt ttatggaaaa aggataataa agaaacaaag
     2041 agaaatctac tgtttttctt gaattctttt tatctttcag gtaaactatt gaatgttgag
     2101 cagaaaagta tatttgctct tgcgatacgg atcttttgtc tgtataatag ctgagggtgc
     2161 tttatgcatc agcaaatcca cattcttatt ggattgtcgg taggtgcttg aaaaagtttg
     2221 ccggcaaggt agaagataag aagaggaaaa ataaaaccaa atcggaggaa acaaacatgg
     2281 cagtaatttc tatgaaacaa ttactagaag ccggcgtaca ctttggtcac caaactcgtc
     2341 gctggaatcc aaaaatgaag aaatatatct tcacagaaag aaacggaatc tacatcattg
     2401 acttgcaaaa aacagttaaa ttagtagatg cagcttatga ttacatgaag agcgttgctg
     2461 aagaaggcgg cgtagcatta ttcgtaggta caaaaaaaca agcacaagaa gcaatcaaag
     2521 aagaagcaac tcgtgcagga caatacttcg ttaaccatcg ttggttaggc ggaacattga
     2581 caaactggga tactatccaa aaacgtatca gccgtttgaa acaaatcaat gcaatggaag
     2641 aagatggaac ttttgaagtt ctacctaaaa aagaagttgc tggtttgaac aaagaacgtg
     2701 aacgtcttga aaaattctta ggcggtatcg ctgatatgcc aagaatccca gatgtaatgt
     2761 acatcgttga cccacgtaaa gaacgtattg ctgttcaaga agcacacaaa ttaaatatcc
     2821 caatcgtagc tatggttgat acaaactgcg atccagatga aatcgacgta gtaatccctt
     2881 caaacgacga tgcaattcgt gccgttaaat tgattacttc aaaaatggct gatgctttca
     2941 tcgaaggaaa ccaaggtgaa gatcaagtag ttgaagaaga tttcacagaa gaaaacacag
     3001 caacatcaat cgaagaaatc gtagatgttg ttgaaggcga caattcttca gcagaataat
     3061 tttgaatgat ggagctgttt caaaggctgg gcgacaagcc tcactctcct ttgaagcagc
     3121 tttttttaaa aagaaaaaca cacaggagga gttaaacatg gcagacgtta cagctaaaat
     3181 ggtaaaagaa ctacgcgaaa tgactggcgt aggaatgatg gatgcgaaaa aagcattagt
     3241 tgaagtagaa ggcgacatgg aaaaagcagt tgatctttta cgtgaaaaag gaatggcaaa
     3301 ggctgcgaag aaaaatgacc gcattgcagc tgaaggattg gcttcagttg ctgtgaaagg
     3361 taacactgca gcaatcgttg aagtaaactc agaaacagac tttgtttcta aaaacgaaat
     3421 gttccaagat ttagtaaaag aaattgcaga acttgttgct gaaaacaaac cagcagacat
     3481 ggaagctgca atgaaaatca aaacagataa aggtacaatt gaatctgact tgatcgaagc
     3541 aactcaagtt atcggagaaa aaatcagctt ccgtcgcttt gaagttgtag aaaaagacga
     3601 caatgctgca tttggtggat acttgcatat gggcggacgt attgccgtat tgactgttct
     3661 agatggaaca actgacgaaa cagttgcaag agacgtagct atgcacgtag cagctatcaa
     3721 tcctcgttat gtaaacgaat cacaaattcc agaagctgaa ttagaacatg aaaaaactgt
     3781 tcttacagaa caagcattga acgaaggcaa accagcgaat atcgttgaaa aaatggtaga
     3841 aggacgcttg aagaaattca aagcagaaat cgctttggtg gaccaaccat tcgttaaaga
     3901 tcctgatatg actgttgaaa aatacgttgc atcaaaaggc gcaactgtta aaacattcgt
     3961 acgtttcgaa gttggcgaag gtatcgaaaa acgcgaagat aactttgtag aagaagttat
     4021 gagccaagtg aaaaaataat tctgttgaat aacagacggg aacgcacggt ttgtgcgctc
     4081 ccttttttta agcaaaacaa gaaaaaccag tcttagagtg tcgaaatcat ctaagtatgg
     4141 taaaatggca atagaaaata ttggaggaat gaacatggtc acaccgaaat atcagcgtgt
     4201 agtcttaaaa ttgagtggag aagctttagc tggggacgca ggatttggaa tcaaaccacc
     4261 agtaatcaaa gaaatcatcc aagaaatcaa agaagttcac gagctgggcg ttgaaatggc
     4321 aatcgttgtg ggcggcggaa atatctggcg cggacaaatc ggagcacaaa tgggcatgga
     4381 acgtgctcaa gctgattaca tgggtatgtt agctacagtt atgaatgcct tagctttaca
     4441 ggatacgttg gaaaacgtgg gtgtaccgac acgcgtgcaa acttcgattg aaatgcgtca
     4501 aatcgcagag ccatatattc gccgtaaagc agaacgtcat ttagaaaaag gacggatcgt
     4561 tatctttgca ggcggtaccg gcaacccgta cttctcgaca gacacaacag cagcattacg
     4621 cgcggcagaa atcggcgcag atgttatcct gatggctaaa aacaatgtag acggtgttta
     4681 ttctgctgat ccgaaagtag atgcaaatgc agtaaaattt gaagaactga ctcatctgga
     4741 agttattgcc aaaggattac aagtcatgga ttcaactgca agttcactaa gcatggataa
     4801 tgatattcca ctgcttgtct tcaatttaaa tgaacacgga aatattcgcc gtgcaatctt
     4861 aggcgaaaat atcggaacaa ctgtaagggg gaaataaaaa tggcagatgc aattatgaca
     4921 gaagcaaaag aaaaaatgaa aaaagcagca caaaatcttc aacgtgaact tgggcaaatc
     4981 cgcgcaggcc gtgcgaacgc tagccttttg gaccgtatta ctgtgaacta ctacggtgcg
     5041 ccaacaccat tgaaccaaat ggcatcaatc caaattccag aagcacgtgt tttaatgatc
     5101 acaccttttg acaagagcat tcttcaagat gttgaaaaag ctattatggc aagtgatatc
     5161 ggaatcagtc caaccaatga tggaaatgtc attcgcttag ttatcccgca actgacagaa
     5221 gaacgcagaa aagaattagc taaagatgtt aaaaaagaag cagaaaatgc aaaaatcgcg
     5281 attcgtaaca tccgtcgtga tgcgatcgat gaatataaaa aacaacaaaa aaatggtgat
     5341 attaccgaag atgatttgcg cggattagaa aaagacgtac aaacattgac agataacagc
     5401 atcaaagaag tagataagat cgctgctgat aaagaaaaag aattattgga agtttaataa
     5461 aatcataaaa acagaggggc ggatcattcc gcttcctctg tttttattta tatttctttt
     5521 atttaaaaat taaaaaaaga aaaaattatt cagtttttaa ataaaaaaac aatgatttca
     5581 taggaaaaac agttagatat tgctataata cttaagatag tgggaatctt ttaagtatag
     5641 gaggaaaaag aatgttacgt ttttttccgc aaaaaaacaa atatattcaa gaaaaaagcg
     5701 aatttcaatt taataccgat ggaccaattc ctcagcacat tgccatcatc atggacggca
     5761 atggtcgttg ggcgcaaaac cgtcgtttgc cacgagttgc tggccataaa gaaggcatgg
     5821 aaacagtaaa gaaagtcaca aaaaaagcat caaggcttgg agtgaaagtg ttgacgcttt
     5881 atgccttttc gactgaaaac tggaagcgac caaaagatga agtaagtttt ctcatgcaac
     5941 tacctgtcga cttctttgat acgttcgtac cagaattgat taaagaaaat gtcaaagttc
     6001 atgtcatggg ttatgaaaac gtattgccag aacatacgca agatgctgta agacgtgcaa
     6061 ttgagcaaac aaaaaacaat actggaatgg tattgaattt tgctttgaat tatggaagca
     6121 gagcagagat cgtgactgct gtccaagaaa ttgccgaaga agtagcaaaa ggagaaatac
     6181 acgcagaaga aatcgatgat gaattgattg ctaagcattt aatgacaggt ttcttgccta
     6241 aagagttaca agatcctgag ctgatgatcc gtacaagtgg agaagagcga attagtaatt
     6301 ttctgttatg gcagatcgca tatagtgagc tgtattttac aaaagcgctt tggcctgatt
     6361 ttgatggtgc gcatcttgaa gaagcaatcg cctcttatca aaatcgagac cgtcgattcg
     6421 gcggtgtaaa aaaaacagaa gatgagaaag gggatcagtc atgaaacaac gtgtggtcac
     6481 agcagccgtg gcgctagtct tgtttattcc gattatatgg atcggaggca tagcggttga
     6541 gcttacagca gcaatcttag cagtaatagg agtttacgag ctgtttcgga tgaaaggatt
     6601 gacactctta agttttgaag gagttttatc cgccgttggt gcagtgttac ttgttttgcc
     6661 aaaagaaaga tggtttttct ttcttccaga gaatgtaagt acatttatgt tattttatct
     6721 tatagttatg attcttttag gaacatcagt tatttctaaa aatacgtata cgattgatga
     6781 agcaggtttt cctgtgcttg tcagcttgta tgtgggaata ggtttccaga attttgtcaa
     6841 tgcacgaaca gaaagcttgg ctgttttgat atttggtcta tttattgttt gggcaactga
     6901 tatcggagca tatatggttg gtcgacgtta tggtcagcat aagttaagtc ctgatatttc
     6961 gcccaacaaa acgattgaag gtgcgctcgg tggtattggt agtgctgttg tagtcgcatt
     7021 tctttattta ttgctatacc cagcaaaaga actatttaat catggtatgt ttgtcatgct
     7081 tctgatgacg attatttttt cagcagtcgg acaatttggt gatttggtcg aatctgcaat
     7141 caaacgtcat tatgatgtga aagactcggg aaatatctta ccaggccatg gtggaatctt
     7201 agaccgtttc gacagtttgc tgtttgtatt tcctatcatg catttatttg gcattattta
     7261 gtaagtatac ctgcgtttct ttgtgaattt cataaaagac gcaggttttt atttttcatt
     7321 atattgatac tattggtgtc tttggtatac tatggtaaaa ttatagtgtt ttatcgttgt
     7381 tttataaaaa acacaaaaaa caagtaaagg agaaaaaagc tatgaaaacg attctgacat
     7441 ttatcatcgt ttttggtata ttagtgattg ttcatgagtt tggtcatttc ttctttgcga
     7501 aacgatcagg aatcctcgtt cgcgaatttg ctatcggtat gggtccgaaa atttatgggc
     7561 atcaagcaaa agacgggaca acatatacgc ttcgcctatt acctattggc ggttatgttc
     7621 gaatggcagg caatggggat gatgaaacag aaatggcacc aggaatgcct ctttctcttt
     7681 tactaaattc tgacggcatc gtagagaaga taaatttaag taagaaaatc caattgacaa
     7741 atgctatccc gatggaattg agccgttatg atttagaaga tgagctgact atcactggtt
     7801 atgtgaatgg agacgaaaca gaagtagtaa cctaccctgt tgatcacgat gcgacgatta
     7861 ttgaaaacga cggaacagaa atcagaattg cgccaaaaga cgtacagttc caatcggcta
     7921 aattgtggca gcgtatgctg accaactttg ctggacccat gaataacttt attttggcaa
     7981 tcctgttatt catcattctg gcatttatgc aaggtggtgt tcaagtgacg aacactaatc
     8041 gcgtaggaga gatcatgcct aatggagctg cggcagaagc aggcttaaaa gaaaatgatg
     8101 aggtagtcag tgtggacgga aaagaaatcc attcatggaa tgatctgaca acagtcatta
     8161 cgaaaaatcc ggataaaaca ttagacttta agatcgaacg agaaggtcag gtgcaatctg
     8221 tcgacgtgac accgaaatct gtagagtcaa atggcgaaaa agttggccaa cttggtataa
     8281 aagccccaat gaatacaggt tttatggata agatcattgg agggacgcgt caagctttca
     8341 gcggttcatt ggaaattttc aaagccttag gatcactatt tacaggtttc agtttagata
     8401 aattaggcgg acctgtcatg atgtaccaat tgtcttcaga agcagcaaat caaggtgtaa
     8461 caactgttat tagtttgatg gcgcttcttt caatgaatct cggaatcgtc aatctgcttc
     8521 cgattcctgc cttagatggc gggaaattag tcttgaatat ttttgaaggg attcgcggga
     8581 aaccattaag tcaagaaaaa gaaggaatat tgacattagc tggctttgga tttttaatgt
     8641 tgttgatggt gctagttaca tggaacgata ttcaacgatt ctttttctag aaaaagttga
     8701 caaatagggt ataataacaa acgataacta ttttgaggaa ataaaggagt atatagaatg
     8761 agacaatcaa aaatgttgat tccaacatta cgcgaagttc caaatgatgc agaagtatta
     8821 agccatcaaa tcttgctaag agctggatat atccgccaag tatcagcggg tatttattca
     8881 tatctgccat tagcaaatcg tgtattggaa aaactgaaac gcattatgcg ggaagaattt
     8941 gaaaaaatcg gagcggtaga aatgctgatg cctgccatct tgccagcaga actttgggaa
     9001 gaatccgggc gttacgaaac atatggacca aacttgtatc gtttcaaaga ccgcaatgat
     9061 cgaaaaatga tccttggacc aactcacgaa gaaactttta ctgatttgat gcgtaatgaa
     9121 atcaattcat ataagaaatt acctttaaat ctttatcaaa tccaacctaa atatcgtgac
     9181 gaaaaacgac ctcgcttcgg tttgcttcgc ggaagagagt ttatcatgaa agatgcgtat
     9241 tctttccatg caacggaaga aagtttggac gagacatata aagattacga aaaagcctac
     9301 accgaagtat tcaagcgttg tggcttagaa tttcgctcga ttatcggaga cggtggcgca
     9361 atgggcggga aagactcaaa agaattcatg gcgatctcag aaatcgggga ggatacgatc
     9421 tgttattcga ctgaaagtga ttatgctgca aacttggaga tggctacaag ctactatgta
     9481 tcaaaaaaat cgcatgaaac acagcttgaa ttggagaaag tagctacacc taacgtaaaa
     9541 tcaattgacg aagtagcaga tttcttttct gttgaacctc aaaaaatcat caagtctgta
     9601 ttgttcattg cggatgaaca acctgtcctt gttttggtac gcggagacca tgaagtcaat
     9661 gacgtaaaac tgaaaaactt tttatctgct gactttttag aagaagcaac agaagaagaa
     9721 gcacaaaaat atctaggtgc tgagtttggt tctgtcggac cagtcggtgt ttcagatgac
     9781 gtaaaagtat atgcagaccg ttatgtccaa gatttagcga atgccattac tggagcgaac
     9841 gaaacaggat tccactataa aaatgtgaat ccagaacgcg atttcactgt actttcttat
     9901 gaagatcttc gttttgtaca agaaggagat ccttcaccag acaacaatgg tgttcttgcc
     9961 tttactcgag gaatcgaaat cggacatatc ttcaaaatcg gcactcgtta cagtgaatct
    10021 atgggagcaa ctgtactaga cgaaaatgga cgagaaaaat ttgtgattat gggctgttat
    10081 ggtatcggtg taagtcgttt attatcagca atcgtagaac aacattcaga tgaacaagga
    10141 atcaactggc caaaaggaat tgcgccattt gatttgcata tcgtccaaat gaatctaaaa
    10201 gacgaatacc aaacaaacct gacgaatgaa ctagaagaag cgatgacaaa agctggctat
    10261 gaagtactgg ttgatgacag aaatgaacgc gcaggagtga aattcgcgga ttctgatttg
    10321 attggctgtc caatcagaat cactgtcggt aaaaaagcag tggatggaat cgtagaagtg
    10381 aaaatcaaga aaactggcga aatggttgaa gtacgtaaag aagaattggt gaatacttta
    10441 ccaatcctct tgaaccaagg cgaataataa gcgttaaaac acagaaaata aaaatagctg
    10501 aatcagctgt ttttgacaat ctgatgattc gcttttaaaa ttggaactgc gatttgaatc
    10561 gcagttccaa ttttttaatg taaaactcag aaaaaacgag tatactaaaa ggtactgaaa
    10621 aagaagagga taagatgcga tcaattgcat tgtttctcat cagaaagggc gtatatcttt
    10681 gtcagaaaaa cgagatttat ttgaaaaact gttggaacaa atccaactag aggaagcaga
    10741 aaaaaatcat ccactgctta ctgctggtga aatggatagt gtagtggtcc atcgtaaatc
    10801 gcgtttatgg gaatttacgc tgcatttttc caaaattttg cctattatgc tatatcggtc
    10861 attgactcag catctgacta tcgcttttaa agatattgca caagtcaaac tgaatatctt
    10921 agcagatgac cagacattcg atgaacagct cttgcaagat tactgggccc aagctttgga
    10981 aaatcagcaa tgcgatacac cactagcaca gcaagtattg aaaacgcagg tacctgtgat
    11041 aaaagatcgt aaagtcatct tgccaatcga cagtacagga gctatttctt atttgaagca
    11101 acaatacttg ccattagtag aagagttgtt cgtcagttac ggattcccaa aatttcgtat
    11161 cgaaccagaa gtggatgagc agcaagcgga acgagtattg aaactttttg aagaacgcaa
    11221 acaagaacag gcagaagcct ttatgaagca agcagctgaa agtctagttg tccacgaaca
    11281 aaagaaaaag gaaagaaaag agcaatcgcc agctcttgaa ggacccatcc acatccagct
    11341 aggacgaaat attccagccg atgaaccaac gacaccaatg atcagtatcg ttgaagaaga
    11401 acgaagagtc acacttgagg ggtacgtatt cgacaaagaa gtcagagaac tacgttcaaa
    11461 acgcaagatc ttgactttga aaatcacaga ttatacttca tcatttatcg tcaaaaaatt
    11521 ctcgaacggc gaaaaagatg aacaagtatt tgatgcgatc agtgtcggta gctggttaaa
    11581 ggtacgagga agtattcaag aagatacgtt tgtccgtgat ttggtcatga atgcgcaaga
    11641 tatcatagaa gtcaaacata cacctcgcaa agattatgct cctgaagggg aaaaacgagt
    11701 agagcttcat gttcatagca atatgagtac gatggatgca acaaacagca tctctgatct
    11761 tgtggcacaa gcagggaaat ggggacaccg agctatcgcc attacagacc acggtggtgc
    11821 gcaagccttt ccggaagcac atagtgctgg aaaaaaagca ggtgtgaaaa tcttatacgg
    11881 cgtggaagcg aatgtcgtag atgatggtgt cccaattgct tataatgatg cacatgaagc
    11941 attgagtgaa gccacatatg tcgtatttga cgtggaaacg acaggattgt ctgcagtata
    12001 tgacacgatc atcgagctag ccgcagtcaa aatgtataaa ggaaatgtga tcgaaagctt
    12061 tgatgaattt atcgatccag gacatccttt gtcacgaaca acggtagatc tgactggtat
    12121 cacggatgga atggttcgag gatcaaagtc ggaagaagaa gtattgcgga tgttccttga
    12181 attttcaaaa gatacaattt tggtagccca caacgcggct tttgatatgg ggttcttgaa
    12241 tactagctac gcaaggtatg gtattcctga agcggccaat ccagtcattg atacgttgga
    12301 attagctaga tatctgtatc ctcaattcaa acgtttcgga ttaggtgtgt tatctaaaaa
    12361 atttggtgtc agcttggaac agcatcaccg agcaatctat gatgctgaag caactggtca
    12421 cttagcatgg atctttgtga aagaggcaat ggacaatcat aacatgcttt atcatgatca
    12481 gctaaatgag catatcggag aaggagattc ttacaaaagg gctcgacctt tccatgtaac
    12541 gattttagca aaaaatcagg caggactgaa agatttgttc aaactgattt caatgtcgaa
    12601 tgtcgaatac ttcgaacgcg tcccacgaat accacgctcc caattgaaaa agatgcgtga
    12661 aaatctattg atcggctcag cttgtgataa gggagaaata ttcgaagcca tgatgcaaaa
    12721 aggggtagaa gaggcaagaa accgagcaaa attctacgat tatattgaag ttatgccaaa
    12781 agcggtctat gcgcctttga tcgagcaaga attggtaaaa aatgaacatg atttggaaga
    12841 aattatccaa aatttggtag aaatcggaaa aagtttggat aaaatcgttg ttgcaacagg
    12901 taatgtacat tatctgaacg aagaagatgc catttaccga aaaattttga tcaattctat
    12961 gggcggagcg aatccgttga atcgtcatag tttgccggat gtccatttcc gaacaaccga
    13021 tgagatgcta actgcatttc actttttagg ggaagaaaca gcgaaagaga tcgtagtaga
    13081 aaatacgaat aaaatcgcag atatctgcga agaagtgatt ccagtcaaag atgaacttta
    13141 tacaccgaag attccaggtt cagaagatga aatatcagaa ttgagttaca caaaagcaaa
    13201 acaaatgtac ggagatccgt tacctgagat cattcaaaaa cgattaaaaa aagagttgaa
    13261 ttctattaat ggtaatggct tctcggttat ttatctgatc gcccaaaaac tagtgcataa
    13321 aagtaatgaa gatggctatc tagtaggttc acgtggctct gttggctcta gctttgttgc
    13381 gacgatgaca ggaatcactg aagttaatcc gttagcccct cattattatt gtccagagtg
    13441 ccaatattcc gagttttttg aagatggtac gtatggttct ggatttgaca tgccagaaaa
    13501 acaatgtcca aaatgtggtg cacgcttaaa caaagacggt catgatattc catttgagac
    13561 atttttagga ttccatggag acaaagtgcc cgatattgat ttgaacttct caggtgatta
    13621 tcaagcagaa gcccataact atacgaaagt attgtttggc gaagactatg tttaccgtgc
    13681 cggaacgatt ggtacagtcg ccgacaaaac agcatatggc tatgtaaaag gatacgaaag
    13741 ggacaataat ctgcagtttc gaagtgcaga agtcgacaga ttagcaaaag gcgctactgg
    13801 agtcaaacgg acaaccggcc agcatccagg ggggatcatc gttattcccg attatatgga
    13861 tgtctatgat tttacgccga tccaatatcc agcagacgat caaaattcgg aatggaaaac
    13921 gactcatttt gattttcact cgattcacga taatgtattg aaattagata ttctagggca
    13981 tgatgaccca actgttattc ggatgcttca agatttatca gggatcgatc cacaaacgat
    14041 ccctacggat gacccggaag tgatgcggat atttgccggt cctgaagtac ttggagtaag
    14101 tcaggaacaa atttattcca aaacagggac attaggtatt ccagaattcg gcactcgttt
    14161 tgttcgaggg atgctagaag aaacgcatcc tacaactttt gcagaactac tgcaaatttc
    14221 cggactatct catggtacag acgtatggtt aggaaatgcg gaagaattga ttcgacgagg
    14281 agatgcgaca ctggcggaag taatcggctg tcgtgatgat attatggttt atttgatcca
    14341 tgctggatta gatagcggga tggcatttaa aatcatggaa actgttcgta aaggacaatg
    14401 gaacaagatc cctgatgaat tgcgagaaac ctatctatcg gcgatgaaag aaaataatgt
    14461 gcccgactgg tatatcgatt cttgttcaaa aatcaaatat atgtttccga aagcccatgc
    14521 tgcagcttac gtattgatgg ctcttcgtgt cgcttatttc aaggtttatt tccctatttt
    14581 gtattactgt gcatactttt ctgtacgtgc agacgacttt gatctagtct ccatgtgcaa
    14641 aggaaaagat gctgtgaaac aagcaatgaa agaaatcacg gacaaaggat tagatgcttc
    14701 cgtaaaagaa aagaatcaac tgacagtgct ggagttagcc aatgaaatgc tggaacgagg
    14761 attcaaattt ggaatgattg atttgtataa atcggatgcc gttaactttg ttattgaagg
    14821 tgatacgttg atcgcaccat ttagagctgt cccaagtttg ggaaccaacg tggcaaaaca
    14881 aatcgttgaa gcaagaaaag acggtccatt tttatccaaa gaagatcttg ccacccgcgg
    14941 taaagtttca aaaacattga tcgaatatat gaacgacaat ggcgtattga aagatctacc
    15001 agatgaaaat caattatcgt tattcgatat gctataagca tctgttttcc tgtctgcttg
    15061 taattttaag cagagtatgg tatagttctt ataggtaatg atactaactg tgagtgagcg
    15121 gatttttcgc tcactctttt taatggaatt acgcacttaa ttttttaagg aggcgaaact
    15181 tttgagtagc gtcgttgaaa cagtcacaga aatggtgaca ccgattttag atgaacaaaa
    15241 ttttgaactt gtcgaagtcg aatttgtaaa agaaggaaaa agctggttct tacgagtatt
    15301 tattgacaaa gaaggcggta tcgatatcga agaatgcgcc tttgtcagtg agaaactgag
    15361 tgaaaaactt gatgcagcag atccagatcc gattcctcaa gcttatttcc tggaggtatc
    15421 ctccccggga gcagagcggc cattgaaaaa agaaagcgac tatgaaaagg cacttggtga
    15481 ttatgtccat gtctccttat accaaccagt agatggcgaa aaacaatatg aaggatttct
    15541 tcaatcattt gatgctgaac agcttacatt gaaaatacgc ataaaaacac gagaaaaaga
    15601 attgactttt gaccgaaaaa atattgcaaa agctcgctta gctatccaat tttaatcacg
    15661 gagaggaatc agaaaaaatg agtaaagaaa tgttaaatgc gttagacgct ttagaagctg
    15721 aaaaagggat ctcaaaagaa atcgtgatcg atgctttaga agcagcattg gtttctgcat
    15781 acaaacgtca ctatggccaa gcacaaaacg tcgaagtgga atttgatcag aaaaaaggaa
    15841 agatccatgt ctatgcggta aaagaagtaa cggaagaagt gatggattca cagttggaag
    15901 tctcattgaa agatgcactt ttaatcaatc cagcatatga gatcggtgat acgatccgtt
    15961 ttgaagtaac accaaaagat tttgggcgga tcgctgcgca aacggctaaa caagttattt
    16021 tacagcgtgt acgtgaagca gaaagaacga ttatttataa cgaattcagt gcttatgaaa
    16081 aagacatcat gcaaggaatc gtcgaacgcc aggataaacg ttatatttat gtcaatcttg
    16141 gtaaaattga ggcagttctt tctaaacaag atcaaatgcc aaatgaattt tatcaaccac
    16201 atgatcgaat caaagtttat gtttcccgag tagaaaatac gtctaaaggt ccgcaagtat
    16261 ttgttagccg cagccatcca gacctattgc gccgcttatt tgagcaagaa gtaccagaag
    16321 tctatgatgg aatcgttgaa attgtcagca tcgcccgtga agctggcgat cgttcaaaag
    16381 tggcagttcg ctcaaatgat ccaaatatcg atgcagtcgg tacatgtgtc gggccaaagg
    16441 gacaacgagt tcaagcaatc gtcaatgaat taaaaggcga aaacatggat atcgtcgaat
    16501 ggaatgaaga tccagctgta ttcattgcaa atgcattgaa tcctgcacaa gtcatggatg
    16561 tgatcttcga tgagacaaat ccaaaggcct gcacagttgt cgttccagac taccaattat
    16621 cgctagcaat cggtaaacgc ggacaaaacg cacgtttagc tgctaaattg actaaccata
    16681 aaatcgatat caaatcagaa tctgacatga cggaatttta tgaaaatcaa gaacaacaaa
    16741 aagaaacaga agaactgcat gatgaagcaa tcgttcagtc agatttaaca gacgatgaat
    16801 atgaaacaat tgcattcaac aatgaaacgg tcgaagaaaa accagaagct taactttagt
    16861 aaggaggcaa agagatgaaa caaaggaaaa ttcctttacg caaatccgtg gtttcaggtg
    16921 aaatgaaacc taaaaaagag atggtccgaa tcacccgttc aaaagaaggg gaagtatcga
    16981 tcgatccaac tggaaaaatg cctggacgag gagcgtatgt ctcacttgag ccggaagaag
    17041 tacaacaagc ttgggataaa aaaattctcg atcgagtact cgaaacaaca ttaacagatg
    17101 agttttatca agaacttctt gactatgtca ctcaccaaaa agcacgtaaa gagttatttg
    17161 gaaaatgaat acagttaatc ggcaaaaagc catgaatctc atcggtctag cgatgcgcgc
    17221 gggaaaacta atcacgggtg aagaactgac cattgctgaa atccgcaaac aaaaagcaaa
    17281 attggtgttt gtggcaaccg atgcaagcga gaatacaaga aaaaagataa aagataagag
    17341 ttcatattat gaagttcctt gctttgagct attttctgaa gcagaaatca cgcagatgat
    17401 cggaaaacct cgcaaggtat tcggaataat ggatgccgga tttgcgaaga agaccaagga
    17461 gctaattgaa ggttaggaag gtgattgcat gggcaacaaa agaatctatg aattagcaaa
    17521 agaactcaaa aaatctagta aagacgtcgt ggaaaaagca caacaacttg gcatcgatgt
    17581 gaaaaaccat atgggtgcga ttactagcaa tgatgagaag aaattgcaac aagcgttcaa
    17641 aaacccaagc acgaaacaac aagctgcaca acaagcaagc agaaaaccag ccaatcaaca
    17701 gccgaacgat caacgtaaaa aagaaacaac gaacaatcaa caaaaaaata aaaataaccg
    17761 cagttatcaa gatcgcggtc aagggagcgg tcaagtgaat caaggaaaaa aaccaacaac
    17821 aaaccaaaat aatacgggaa ataccagcag ccaaaatcgt caaggaacga gccaaggcgg
    17881 caatcaaaac cgtactaaca ataacaacaa accaaacaat aacaatcgcg gcaaatttaa
    17941 taataataac cgtaatcgtt ataataaaaa aggtaaaaaa ggcaaacagc aacaatcaac
    18001 taaaccagct gtaccaccac gcaaattccg tgaattgcca gatgtattag agtatactga
    18061 aggtatgaac gtagcagata ttgcgaaaaa aattcatcgt gagccagcag aaatcattaa
    18121 aaaattattt atgcttggtg tcatggtcaa tcaaaaccaa gcactggata aagatacgat
    18181 tgaactgtta gcgacggact atggcatgga accacaagaa aaagtccaag ttgacattgc
    18241 tgatatcgat aaattcttcg aggcagaaga agtcgatcca gataaattag tctcacgtcc
    18301 gccagtcgta acgattatgg gacacgtaga ccatgggaaa acaactttat tggatacatt
    18361 acgccattca cgtgtaacaa gcggcgaagc cggcggaatc acacagcata tcggtgccta
    18421 ccagatcgat attgatggta aaccaatcac tttcttggat acaccaggac atgcggcctt
    18481 tacaagtatg cgtgcacgtg gagcaagtat cacagatatc acgatcttag ttgttgctgc
    18541 agatgatggc gtcatgccac aaacagttga agcaatcaac catgccaaag ctgcaggcgt
    18601 tccaatcatc gtcgctgtaa ataaaattga taaaccaggt gcaaaccctc aacatgttat
    18661 gcaagaatta agtgagtatg agttaattcc tgaagcatgg ggtggagaaa cgatctttgt
    18721 tgagatctca gcgaaattcg gccaaaacat cgaagaattg ctggaaatga tcctgttagt
    18781 cgcagaagta gaagatttaa aagcagatcc aacacaacgc gcaatcggaa cagtgatcga
    18841 agcacgtctt gataaaggta aaggtcctgt gtcaacctta cttgtccaag aaggaacact
    18901 tcatgttggt gacccaatcg ttgtcggtaa tacttacgga cgtgtccgtg taatgacaaa
    18961 cgatcttggc cgtcgcgata aagaagcagg acctgcaacg ccagtagaaa tcactggctt
    19021 gaatgatgta cctcaagcag gtgaccgttt cgtagtcttc gaagacgaaa agacagcacg
    19081 tgcagcgggt gaagaacgcg cgaaacgtgc attattagaa aaccgtgctg ccacaagccg
    19141 tgtgactttg gataacttat ttgaaagcct gaaagaaggc gaattgaaag aagttaacgt
    19201 aatcatcaaa gcagatgtac aaggttcagc agaagcactg gctgcatcat tgaagaagat
    19261 tgatgtggaa ggtgtccgtg taaaaatcgt ccattcagct gtcggagcaa tcaatgaaag
    19321 tgatgtaacc ttagcagcag ccagcaacgc tattatcatc ggtttcaacg ttcgtcctac
    19381 accgcaggca aaagtgcaag ctgatactga agaagtcgat attcgtcttc accgtatcat
    19441 ctataaagcc atcgaagaaa tcgaaactgc gatgaaaggg atgctagacc ctgaatttga
    19501 agaaaaaatc accggtcaaa tgatcgttcg tgaaaccttc aaagtttcta aagttggtac
    19561 gattgcaggt gcctatgtaa cagaaggata tattcgtaga gatagcggtg tccgtgtaat
    19621 ccgtgacgga atcgtaatct atgaaggcca acttgcaagt ctgaaacgat tcaaagatga
    19681 cgtgaaagaa gtcaaaatgg gttatgaatg tggtgcaatg attgaaaaat tcaatgacat
    19741 caaagtagat gacgtaattg aaggatttat catggaagaa gtcaaaaatg actaaaaact
    19801 gataaaagga ggccaatcat tatggctaac taccgtgacc gccgagtcgg tcaagaaatc
    19861 ttaaaagaag tcaatgatat tttgcgcaaa aaggtccgtg accctcgtgt ggagaacgta
    19921 acgatcacag atgtgaaagt tacgggggac ttacagcaag caacgatcta ttacagcatt
    19981 ttatctgact tagcatcaga taagaaaaca gcacaagatg gattgaataa agcatcagga
    20041 ttgattcgtc gagagttagg gcacaatcta agtatttata agacacctga attgttcttt
    20101 gaacttgatg aatcagttgt ctatggcaac catatcgatg aattgcttcg cgacttgaac
    20161 aaagactaat caaaaactcc cgcaaatcct gtgaaatcag gggttgcggg atttttttgt
    20221 tttcattaag acgtattacc taagagttcc atgactttag tcttgcggcg tgtcagctaa
    20281 ccaaaaaaga gaaaaagaaa agtaaaaaat tcgactttct tttttctagt agtatataaa
    20341 tttttgcata tagataaaga tcaagatacc gataacggaa gctagattgt aatctatttt
    20401 agtgacagtc aggtatagcc aagtaactag aagatcagtg gaacctgcac cataaaagtt
    20461 tgagtttgtc ggtccgttga cagttaaaag aaaaatcacg ttgaagttat tgatatttcc
    20521 gattacgccg cttcgatttg gtcacgatcc aaattttgga taatccctgt agaaattagc
    20581 atagtagctg ggataccaat tcacatattg acaaagataa cagagacttt tacataaaac
    20641 ggatcaatca ggaaaggaat tgctcgatcc agccccagtt ttccagcaat gtgttaactg
    20701 gcccagcgtc attcagcaaa ttatgcatga tgaggagcga aatgaactgc ggaacagcca
    20761 ttgtgataac gacaagtttt ttggatcaat gtaaaaaacg tcagcgaaag gaaaaatttc
    20821 gtggtttttt atacctgttc atttggctag atataaaatc caaacaaatg aacagaagtg
    20881 cttattaggt ttcaattggg agtatctgcg ctagaataag agtaataatg aaaaggagtg
    20941 ttatgtatgg attattctag taaagaatac tttgataaaa tgacggcttg gtggcgtgct
    21001 gctaattatc tttctgttgg gcaactttat cttaaagata accccttact gagacgtaca
    21061 ctgaaacccg aagatgtcaa aaaacatcca atcggtcatt ggggaacgat tcctggccaa
    21121 aattttattt atgtccattt gaaccgagtc atcaataaat atgatttgaa catgttttat
    21181 attgaagggc caggacacgg cggacaagtc atggtatcga atgcttattt agatggaagt
    21241 tacacagaga tctatccaga agtaacggaa gatgagacag gaatgcaaaa attattcaaa
    21301 cgattttcat ttcctggagg cattgcttct catgcagcac cagaaacacc aggttctatc
    21361 catgaaggcg gagagcttgg atactctctt tcacatgcag tcggagctgt attagacaat
    21421 cctgaagtaa tctctgctgt cgtgattggt gatggtgaag cagaaaccgg tcctttagct
    21481 ggaagctggt tctcaaatgt ctttatcaat ccagtgatag atggagctgt tttaccgatc
    21541 cttcatttaa atggtgcaaa aatagccaac ccaacgatct tggcacgtaa atcagatgga
    21601 gaattagcaa attactttaa cggacttggc tgggaacctt tctttattga aggaaatgat
    21661 tcagaaaaat taaatcctgt tatggctgaa aaaatggatc aagcaatcga gaaaattaaa
    21721 tccatccaaa aagaagctcg cctgaagaca gcaacagatg tcgtgatgcc aaaatggcca
    21781 gtattgatcg tacgcacacc aaaaggatgg accggtccaa aagaatggga cggggaacca
    21841 attgaaggaa ccttccgagc acaccaagta ccgattcctg tagatcaaga acatatggat
    21901 catgcagatg ctttactccg ttggctgaaa tcctatgaac cagaaaaatt atttgatgca
    21961 caaggacgta ttttagaaga aatcagagaa atcgcgccta ctggtgacca aagaatggca
    22021 aaaaatccta tcaccaatgg tggaatcgat ccaagaccgc ttattatgcc agattggaaa
    22081 aaatatactt tgcaatttga aaaaccaggc tccgttaaag cagaagatat gaccgaactt
    22141 ggtaaattcg ttcgcgagat cattgagaaa aatccagaaa atttccgtat atttggacca
    22201 gatgaaacaa aatcaaaccg attgaatcaa gttttcaaaa cgacaaatcg ccaatggatg
    22261 gaaaaaatcg aaccagaaaa tgatgaatgg ctttcgccat ctggtcgcgt catcgacagt
    22321 caattatcgg aacaccaaga tgaagggttc ttagaagggt acgttctgac tggacggcat
    22381 ggttttttgc aagttacgaa tcattcttgc gagtggttga ctctatgttg actcagcact
    22441 tcaaatggat gcggaaatca catgaccttt cttggcgaaa tgattatcct tcgctaaatc
    22501 tcattgcaag ctcaacagtc ttccagcaag atcacaatgg atatagccac caagatccag
    22561 gaatattgac acatttggca gaaaagaagg cggagttcat ccgtgaatat ctgcctgcgg
    22621 atgcaaatac attgctcgca gtcatggata aagcattccg ctcgtcagaa aaaatcaatt
    22681 tgatcatctc ttccaaacat ccgcgtgctc aattttactc agcagaagaa gcagccgtct
    22741 tagtgaatga aggcttgaaa atcattgatt gggcttcgac agcaaaagag gaggaacctg
    22801 aattggtcat tgctgctgca ggaacggaat ctaacttaga agcattagca gctgtaactt
    22861 tgctgctgga agaattccca aaactaaaaa ttcggttcat caatgtcgta gatttattga
    22921 aattacgtca tccaagtcaa gatccgcgtg ggttgagcga tgaagaattt gatcaatatt
    22981 ttacaaaaga caaaccgatc ttatttgctt tccacggtta tgaaacattg gtccgcacga
    23041 ttttctttga tcgtcataac catcatttaa tgattcatgg ctacaaagaa aatggagata
    23101 tcacgacacc atttgacatg cgtgtagtaa atgaacttga tcgttatcat ttggcaaaag
    23161 acgcagcttt aaaaatcaaa ggaagtcaag cagaagattt tgcgaaaaaa atggatcaga
    23221 aattacaaga acatcaaaac tatattcgtg aaaatggtat tgatctgcca gaagtattgg
    23281 attggaaatg gaaaaatctt gaccaataaa atgataaaat cgatcccgca aaatagaatt
    23341 ttttacattg attaagaaac tgaaaaaata aataaaaaaa acggaaaaaa gactggaaga
    23401 atggccagtc ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca
    23461 cccataaatc caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa
    23521 aaagcataac atgatttcta tttacgtgag catgaatttt gttacgtttt tattgccgaa
    23581 aaataaaaac cgttcattat gatgtcaaat tcgtcagtaa ttgatgcaat gtgttataat
    23641 aattcagtta aatcaaaaat gaaatggaga aaagacatgg acgggttatt acctttatgg
    23701 aaagaacgcg gaatgacaag tcatgattgc gtattcaaat taagaaaaat cttacatacg
    23761 aaaaaaatcg gacatggggg tacgcttgac ccagacgtag acggtgtgtt gcccatttgt
    23821 attggaaaag ccacaaaagt tatcgaatat ttgacagatt ctgggaaaat ctatgtaggc
    23881 gagataactc taggtttcag tacaacgacg gaagatcgat ccggagaacg ggtagaaaca
    23941 aaaccagtag atatgtcttt atctgaaaaa aaaatagatg aagtgatggc ttcatttatt
    24001 ggtgagatca cccagatacc accaatgtac tcagcggtaa aagtcaacgg taagcggtta
    24061 tatgaatatg cccgcaataa tgaaacagtg gagcgtcctg ttagaaaggc tcagatttac
    24121 cgttttatcc ggacaagtga attgacaaga gacgaagtga caggaaccat ttcttggcgc
    24181 ttcgaagtag aatgcgggaa aggaacctat gtccgcacat tagctgtaga tacaggcaag
    24241 aaacttggtt accctgcgca catgtctgat ctgacccgaa cagcaagtgc cggtatgtcc
    24301 cagaaggatg ctctgacatt agaagaagta gctcaagctg tgaaaaatgg gacaatcgat
    24361 gattgtttgc tgccaataga aaaaggagtg gaacggttca aacgagtaga tattactgat
    24421 gaagtatggc agaaagtaaa aaacggtatg agacttgatt atactgtatt tgcgctccca
    24481 gaaatgccgt cagaagaaat tgctttgttt tataaaggaa aagttgtcag tatttatcag
    24541 ccaaacccga aagaaaaaaa caaattaaaa cctagtaaag tattgagaaa tgaggaataa
    24601 cgaatgaata tcatcaagat cagacatcct taccaatcag aacaaattcc agaagacgat
    24661 gtcgttttag tgctgggatt tttcgacggc gtgcatatcg gacaccagaa agtcataaag
    24721 acaggtatgg aaattgcaca aaaagaagga ttgaaattag cattgatgac tttcaatcag
    24781 catccatcta ttgtctttaa aaagatcgat cctagcagcg tgaaatattt gactagcctt
    24841 aagcaaaaag aagaaaaaat ggccgctttg ggtatagatt atttatatga agttgatttt
    24901 acttcttctt ttgctcattt agcaccgcag gaatttgttg atcaatacat cgtagggtta
    24961 catgcaaaat atgctgtttc cggttttgat tatacgtatg gaccaaaaga cattgcagat
    25021 gtcgctcatt ttcctgaata tgccaaagga agattcaaaa tcgtcacagt ttctaaagaa
    25081 gaagaacaag gtgaaaaaat cagctctacc cgtattcgtg gattactaga cagcggacaa
    25141 gtaaaagaag cagcaaaact attaggttgc acatatgagg tagaaggggt agttgtccat
    25201 ggcgatgcac gaggcagact actaggattc cctacagcaa atgtcaaagt aaagagcacg
    25261 gttcatttgc ctaaagaagg tgtttatgtg tcggaaataa aaattgggga aacatggtat
    25321 ccagccatgg gttcaattgg acataatgat acattcggtg aaggacggga gctgactgta
    25381 gagttgtata ttttggactt taatcaagat atctatggtg aacatgtcgc ggtacgctgg
    25441 aatcaatttt taagagatca agtgaaattt tctagtgtag aggaattgat tgaacaactc
    25501 aaggaggatg aacaagcaac agctgaatat ttttctcagc aataaaacat tatgcataaa
    25561 atctcttgtt tatgtataac tagcaattac aataaaatgg gtgataaata agcccaggaa
    25621 aggggctgtg acaaaagtct tgtcacagcc tttttttcca aataaatggt ttttcagaag
    25681 tagcttcttc ggaaataagc cgaaatttct tgaagctgac cacttctgtc acaacctctt
    25741 tctttataga aataaaagga cactagcaat tcatgaaaac gattgctaga gcgtatcatt
    25801 tgctttagac tatatatctt ggtgtacact cactttaagg cggaaataaa agttatccgt
    25861 aataacaggt ttaggtatga aaaaggagtg ggtactatgt acaaggaatt agaaaacaaa
    25921 gtcgcagtag taactggtgg ctcaaagggg attggaacag cgattgctca acgattcggg
    25981 aaagaaaaaa tgaatgtagt tgtcaactat aactcggata aagaaggagc agaaaaagca
    26041 gtcaaagcaa tcgaagaggc aggcggaaaa gcagtctctg tacaagcaga tgtcagttct
    26101 gaagaaggaa tacagcgttt acttgatacg gctttagaaa attttgggac actggacgtt
    26161 tgggttaata atgctgggat ggaaaatcag catccaaccc atgaacttag ccttgaagat
    26221 tgggaaagag tgttaaaagt caatttgact ggcgttttta tcggaacgaa agcggcttta
    26281 aattacttta cagagaataa taaaaaagga aatatcatca atatgtcttc tgttcatgaa
    26341 cagatcccat ggccaacgtt tgctcattac gctgcttcaa aaggcggggt aaaattattt
    26401 accgaaacgg tagcaatgga gtatgcaccg aagggaatca gaatcaatag tataggccct
    26461 ggtgctatcc gtacacctat taatgcggca aaatttgatg atcctgaaca aaaagaactc
    26521 ttggaaagta tgatcccact aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct
    26581 gcatggttgg cttcagacga atccagttat gttacaggta ttacactatt tgcagatggt
    26641 gggatgactt tatatccttc tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt
    26701 ttattgattg cattgatccc ggcagttggc tgggggatcc agccactgat tttgaaaaag
    26761 attggtggca gaccaacaaa tgagatatta ggaactggta ttggagcggt gcttgtcggc
    26821 ttactcgttc aattattcgc ttctccagct ggtatttcag tgactacatt tttgattagt
    26881 ttactttctg gtgcattttg ggttattgga caaacaggac agtatacatc tttcaacttg
    26941 attggtgttt ctaaaacgat gccaatttcg actgcattac agttagtcgg aacctcgttg
    27001 attggtgtct tcgcttttgg agaatggtct ggaagtatgg ggaaaatcat tggagcagca
    27061 gcaatcattc ttcttgtatt tggttctgct ttaacggcaa tcagtgatga tcctggaaaa
    27121 aaaggcggta tcacaaaagg aattagtcta ttagcatcaa caagtattgg ttattgggta
    27181 tacagtgcat tgcctaaact agttgatgca agcggacttt ccattttctt tccgcaaatg
    27241 atcggtgtct ttttaggggc agtgatttat gtgattttcc gtaaaccaac cgcattttct
    27301 gaagacaaga gttggaaagc aacgatcgtt ggagccatct ttagtgtgtc agcattggct
    27361 tatatttttt cagcaaatga aaatggggtt gctactgcat atatcattac acagctaaac
    27421 gtagttatct ctacattagg cggtatgtac atcctgcatg aaaataagac accaaaagaa
    27481 aaaaaattaa cgattatcgg attattgctg atcgtagtag gaagcatggt cacggtcttt
    27541 atttgaggat aaagatggta aaatttcatt tattaaaaaa atatttgttt gaaccaaaca
    27601 gtaagtactt ataaaaaaat agcgctagaa tttccaacat ctctggaatt tctagcgctt
    27661 tttaattgtt taagatacaa gggaaatatc tgtctggatc aataattttt cattgatcca
    27721 ttgactaaag tttacgacat aattgaagtt accaccgatt agtatctccg catgattgtc
    27781 tttataaaga tcgtattgta aaagacggtc ttttttattt gcgtaaacat cgttatggta
    27841 ctggactaat tgttctcgga attgattgat tgctgtaaaa tcacgatatt gaaggttgaa
    27901 acgcatcgtg attggtgcat ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc
    27961 ttgatctccg tataggaact caaaaagatt acgttcagaa aatttccatt gcccgccaac
    28021 ctttgttcct tttagttttc cttcatgaag ataatttctg atagaacgtg tagtaacacc
    28081 taattgttcg gctaattcat ttactgagta aaaatctttc aatgttcacc ctcctatgtg
    28141 aatggttcta ctgacttagt tgatatttgc ttaatcacga aatgtagaaa gaacgatttt
    28201 aattaaggtc attgtaaaaa tcaagtcaga aaattctaac ttttatttct ctaaagaaat
    28261 tcgttctctc ctaaagtgat attatattta a
//
LOCUS       DAYYDS010000031        26557 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000031 DAYYDS010000000
VERSION     DAYYDS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.031"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..729)
                     /locus_tag="ACHA02_001312"
     CDS             complement(19..729)
                     /locus_tag="ACHA02_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8669668.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="ACHA02_001313"
     CDS             complement(726..2198)
                     /locus_tag="ACHA02_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8669669.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="ACHA02_001314"
     CDS             2336..2809
                     /locus_tag="ACHA02_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8669670.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="ACHA02_001315"
     CDS             complement(2962..3513)
                     /locus_tag="ACHA02_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8669671.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="ACHA02_001316"
     CDS             3618..4148
                     /locus_tag="ACHA02_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8669672.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="ACHA02_001317"
     CDS             complement(4631..5074)
                     /locus_tag="ACHA02_001317"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8669673.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="ACHA02_001318"
     CDS             complement(5090..6250)
                     /locus_tag="ACHA02_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8669674.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="ACHA02_001319"
     CDS             6757..8457
                     /locus_tag="ACHA02_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669675.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="ACHA02_001320"
     CDS             complement(8559..9392)
                     /locus_tag="ACHA02_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8669676.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="ACHA02_001321"
     CDS             complement(9696..11861)
                     /locus_tag="ACHA02_001321"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8669677.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="ACHA02_001322"
     CDS             12121..14415
                     /locus_tag="ACHA02_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8669678.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="ACHA02_001323"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="ACHA02_001323"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8669679.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            15066..16073
                     /locus_tag="ACHA02_001324"
     CDS             15066..16073
                     /locus_tag="ACHA02_001324"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8669680.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="ACHA02_001325"
     CDS             16095..17114
                     /locus_tag="ACHA02_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8669681.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACHA02_001326"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACHA02_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8669682.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18872..20293)
                     /gene="tyrP"
                     /locus_tag="ACHA02_001327"
     CDS             complement(18872..20293)
                     /gene="tyrP"
                     /locus_tag="ACHA02_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8669683.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20536..22413)
                     /gene="tdc"
                     /locus_tag="ACHA02_001328"
     CDS             complement(20536..22413)
                     /gene="tdc"
                     /locus_tag="ACHA02_001328"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8669684.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22704..23960)
                     /gene="tyrS"
                     /locus_tag="ACHA02_001329"
     CDS             complement(22704..23960)
                     /gene="tyrS"
                     /locus_tag="ACHA02_001329"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8669685.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24072..24277)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24540..25841
                     /locus_tag="ACHA02_001330"
     CDS             24540..25841
                     /locus_tag="ACHA02_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8669686.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25910..26509)
                     /locus_tag="ACHA02_001331"
     CDS             complement(25910..26509)
                     /locus_tag="ACHA02_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8669687.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         8059 a         5475 c         4754 g         8269 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca cgccgcaagc ttggatcaag
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggttggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttgttttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaacagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcaga aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgagat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttacctgac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttttcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtgtca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagatc
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtac ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag acgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt tttatttttc ttctaaactt ttttctacct
    18901 caatccccca agaatctttc ttgaagtgat tgatgatcaa tggaataacg aacatgacca
    18961 ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc tgtacctaat gtagcaaata
    19021 cgacaacgat gatagagaag atcagcatgc agctgactac ccaagctaat ccgtttccgt
    19081 ttttaccaat tcggtaagga cgttcgaatt ctggatgttt cttacgcatt ttaagcaatg
    19141 aaacagcaat caaaatgtac acgatacaat aaataacagt tgtagcattt gtaagtgtca
    19201 agaatacagc acttacgtca tccattacac cataaagcaa agcaaataat gaaataacga
    19261 ttgattgtgt tagaacgaca tttctagata cgccgtattt gttttcttta tggaatccga
    19321 attttggtgg aaggaatcct tcacgagcta cttggatgat tgtttttgat ggtccagtaa
    19381 cccaagctga taattgtaaa agcacaccaa taaataccat gaagctgaag atatttccga
    19441 tcacttctgg taagcctagg atttgacagt caatcaagat aggctgcgtg atatttgcta
    19501 attccatttt tccatctggt acgacatttg ctacaagcat cgcattgatg acattcaata
    19561 aaactaatcc gatcaaagcg ataaatacgc cttttgtata attttttgtt gcgtctttca
    19621 aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa aataaatgtg ataccagcta
    19681 agtatttcaa tgaatcaata tgttccaagt cagggaaagc ttttgaccat gaaaagtcac
    19741 ctagatagcc gtttgagact agtccgactt tgaaggctgc caaaacacct aaaacgaaca
    19801 tgatgactac tgggatatat acccctagcc atacaccgat gttcccgccg acttttgcca
    19861 tatcgaattt caagttcaag atcgtgatga tccagtagat aactaaaata caacctaaaa
    19921 cgaaccagtg attattgcca agttctacat taccaaatgt gtttcctaac aatggtccta
    19981 aagtagaagc gaccattacc atacctggga acatttgcac ccataatagc catgcaacga
    20041 caaatcccca tttgctgccc agtgctgttt ttacccataa ctgaggaccg ccttcttgag
    20101 gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg accggcaaaa aacaaaactg
    20161 caaaaattga ataaaaaatc aatgtccaac cgactgcggc taacgtagga atactccgaa
    20221 ccgttgcaca cagtgccatt gtcagaccga taaaggtccc aagacccatt ttacttactt
    20281 cagatttact caccgttgac actctccttg attttttgga taaaataacg gtgtctttga
    20341 agtagtttca gcaataaacg aaacaattca gcgaatattt gaggggattc tttgagtcta
    20401 ttgcttgaag ggtttcttca aagacaacct caaaacaaag ggatcgaaga gcttaggtct
    20461 ccccttgctc ttcagatcca aacatgcgtc agaaacagtt agtattcaag ctgttttcta
    20521 acatgattac cttgcttatt ttgcttcgct tgccaataat tggtctgcgt agattttttc
    20581 caatttttct tgaagagctg ctttgatttt ttcagcatat tcttcaaagt gttcttcttt
    20641 gttcatgtat ggtgtcatta cgctggcacg caatacagtt acttttccgg cacggttcca
    20701 ttcttcatct gagaatccta gttggttcac aaattgtaat gggctgttgc catagtctgg
    20761 gatagcaaag tcagtatgtg aagttaagaa ctcattaccg taaatgcttc ctttcacgta
    20821 tgaagaataa tcatatacgt catggttcaa tttattcatt gcaactaagt catcgttgcc
    20881 tttttctttg aatacatagt ctaccatatt gaaatctgga taagttaatg gatgaacttc
    20941 gatttctttg tctcctactt taaatgacaa gtcattcaag aagttgtaga aacgatgtgc
    21001 accttcgatt gatgcaccca ttaatttacc atatcctgtg acattcaaag gtaagacatg
    21061 gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt gaaccttcca agatataagc
    21121 cccaagtaat gctgggatat ctgcgccttt ttcaaataca tatgtcgcaa agtaagagat
    21181 aacgtctctc atgcggatat ctttgatgac gataccaccg gcagaatatg gaacatagcc
    21241 cattttatgt gggtcaatcg ttacagattc tgcttcttcg attgctttat atgcactgtg
    21301 tacttcttct aagatataat ctttgttttc tgtaaagaca ttgtatttgt aatgaacatc
    21361 ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct aagaaaatcg cacggccgta
    21421 tccgccataa gcagcatcta cgtgtaagta gaagtaaata ccgtcttttt ctaatacgcg
    21481 acgcaatgca acgattttat cgataccgtc gattgctcct tcttctgttg atcctacaac
    21541 accgactaca cctaggattg gtgttttttc agctgctaga ccacgaacga ttttttcaag
    21601 ttcgttgatg tccatgcgat agttgtggtc aactggcact ggaatcactt ggtctaaacc
    21661 gataccgatg atgtcggcag cttttagcca tgaatagtgt ttagtttgtg gaaccaacca
    21721 ttttcctaat ttttctaaat gttttccgct acgtgctgag tgagctttga tttcgtcgat
    21781 tttttctggg acgctgtcta acagattcat gatttcttct gttgacaagt tcattaattc
    21841 ccaatcgctt tttccagcaa ctagttcagg tgtcacttct ttcattgcaa gcggcaatga
    21901 tttgatgttg cgggcatacc aaagtccttc taagtttgct aaagaaccat cagcaacgat
    21961 gtggccccag ccatctttat agctcattaa ttttgcaaat tccattccta cttcttcttc
    22021 catttggctt gttgcaggag aagattcata ggcaacgtta ttaccattcc atagcatagc
    22081 aaaattgtac gctagtaaag atggcatcaa tgtttcagag ttcatatgac cccagtaacg
    22141 tccggcatta tgccaaggaa cggaatgggt acgcatccgc gctgaaattt ctgatagaac
    22201 atcctttgtt ttattcacag tgtgttcaaa actagcactg cttttttctt ctggcgtaat
    22261 aataggcatg tcttgaggca tgtagttttg acgccagcct aaatgttcat ccaccaattc
    22321 attcaacaaa gctttataga tttggccgtt ctcagctttg tccccaatga atagggcatt
    22381 taggtttaga tctttcgaca atgattcact catagtgaaa ccctcctaaa aaaatattta
    22441 aattttgtcg agccctacat tacacctgtt cttctattct gtaaactaag ttttggcctg
    22501 atttttaatc atattttaat atgtatcaat tatccgaatt gttcacactt ttgaaataat
    22561 taaaaaaaac gagggaattt acaaatgtaa actccctcgc aaagattgta ttaacgacaa
    22621 ccagcgttct attttaggat gggtttcaaa attggaatgt tgtaatagta tttccctttt
    22681 tcaaggtaaa aacttactaa attctaatca attactttgg ccaaaaagta atttttctta
    22741 ccttttcgaa taaccacaaa cttaccatcg aattcatcgg atggatttat gacaaaattt
    22801 aaatcggtaa cgcggtcacc gttaatactt atagctccgt ttgaaacatc ttcacgtgct
    22861 tgacgtttag atggttcaat ttttgtggaa acaagcagtt ccacgatatt ttcaggtgtg
    22921 cttgaaattt caacgttcgg cattttaccg aatccttgcg cgatttcttc cgcatttagt
    22981 tctttaatat ttccagagaa caatgcttgc gttatttttt gcgcttcttt caagtcttct
    23041 tcactatgca caaatcttgt aacttcttct gccaaacggc gttgtgcttc acgtttctca
    23101 ggttctgtct ctactttttt cgctaagtct tcgatttctt cttgagacaa gaaagtaaag
    23161 aatttcaagt atttcactac atcgcgatca tcttgattca accagaattg gtagaactca
    23221 aatggtgaag ttttcttcgg atcaagccaa acggcaccgc ctgcagtttt tccgaatttc
    23281 gttccgtcag ccttcagcat caatgggatc gttaaaccga acgcttctac ttctggtcct
    23341 tctaattttc ggattaggtc taatccagct gtgatatttc cccattggtc tgctccaccg
    23401 atttgcaatt gcacatcgta tgttttatgc agatgcaaaa agtcgataga ttgcagaatt
    23461 tggtaagtaa actctgtaaa tgagattcct acttccaaac ggctggcaac gatatctttt
    23521 gctaacatcg tgttgatgtt gaagttttta ccgtagtctc tcaaaaattc aagtaaagag
    23581 atttttgaca accagtcgta gttgttcaca aatgtgatat ttgcatcttt accaaacaat
    23641 tttctcattt gattggaaag tgcgtccacg ttatgttgca cttgttccat cgtttgcaac
    23701 acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc ctgttccgcc gccaatcaga
    23761 atatatggat gatggcctgc caattggaat cttttcatca tcataaatgg aatcaaatgt
    23821 ccgatatgca tcgaatcacc agttggatca acgccgcaat aaagtgaaat gctcttttct
    23881 tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat taatggcgtc gcgccaagtt
    23941 aattcatcaa taatattcat tttactgacc tccgtgtagt ttttttatga aagcaggaac
    24001 ctgctagcat gtgtaattaa ttatttcttc aggataggat aggaaataaa aaaagtccct
    24061 tggcatataa gccaagggac gaaatctcgc ggtaccaccc aatttgtatg tctagatatc
    24121 caacatacct ctcgaacatt taacgctgtc gcgtggcaat cactgccaaa gactcctgaa
    24181 atgtaattcg ctttaaagca tgtactggtt tgcagcgacc accagctttc tgaaacattg
    24241 gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt attaatatgt gtctatcata
    24301 gagcgcaact ttttctttgt caacagaaaa aggatatttt ttttaaattc ctcatttttt
    24361 cttttttgaa tccgctctaa ttccgaacat tttattttca attattgtta aaagaaataa
    24421 ctttttctgt attttttctg caatttatcg agaagtcact ttactttgcc tcttattctt
    24481 tcttcgttac aataaggaat gggacaagta aaaaaatgaa aaagcaaggg acgaggtata
    24541 tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc tttaactatc gttattttaa
    24601 ttctaagttt gttttttgtc atccgatcat ctacaaaaaa agaaccaatc attcctgaaa
    24661 aaaaaataac agaaaaagca aaagaaccga cagatgataa gcaacaacta gttactcaag
    24721 caaaaaatct ggctcaaagc tacgattatg accaagcgat tgcgttattg gaaaaagata
    24781 gtaagaacgg atctactgaa acacaacagc ttcttcaaac actaaaaaag gagaaagaac
    24841 aactgatcga ctggtctgat cctactcaga tctctcatgt ctttttccac agcttgatcg
    24901 tggacccgca aaaagccttc cactctcaac aggcacaagg atacaaagat tatatggtga
    24961 ctgttgaaga attcaaccgt tcgatcgaac aactttatca aaatgatttt gttttagtta
    25021 atttaaatga tttgatccaa aaagacgaac aaggaaatct ttcctttaac gggcttgcgc
    25081 tacctgaagg aaaaaaacca ttggttcttt cacaagatga tgtcagctat tatgaatata
    25141 tggataattc cggttttcct agccgattgg tcgtatcaaa taacaatgaa atcaaaaatg
    25201 tctataaaga gaaagataaa gaagaaatcg gtgactttga catggttcct ctgatcgatg
    25261 cctttgtcaa gaaacatcct gatttttctt atcgaggcgc aaaaggtacc ttggccctga
    25321 caggttacaa cggagtttta ggttatcgaa catcaaaatc tcaatatgga gacaatgaaa
    25381 aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga acagttgaag aaagacggct
    25441 ggagctttgc ttcccataca tggggtcact tgaatatgac tcaagcttca ctagaagatg
    25501 taaaaaaaga caatgaattg tggcagcaag aagttgcacc tatcctagga aaaacaaata
    25561 ttctgattta tccatttggt gcagacatca gcgactggca gccttattca gcagaaaatc
    25621 aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt ctgtaatgtc gatgcttcta
    25681 caccagcttg gggacaatta ggtacggatt tctatcgaaa tgctcggatc aatatcgatg
    25741 gcattcgttt tcaatctgat ttagacggaa aaaatcctat tcttgatcca ttcatcaatg
    25801 tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg attttacgtc atttatgaac
    25861 caaaaaagcc gaactgacat tttatcgtca gtttcggctt ttttatgctt tatttcttct
    25921 tcggtgcgat tcccttgatc aggacttctg tccattctgc tgtatatgga agcagttttg
    25981 cgtatatctc cggcttagaa attccttcta ccattgcttg aaaatagagg atcaagaact
    26041 catccgaata tttcaaatcc acttttcctt ctttccgccc tcgatggaaa agatcaagca
    26101 tgatactgaa actttcttgc gtatacgcat ccatcgcgtc agaaaattct tttttttctt
    26161 tttttgtata gtatgtcatc aaatcgatat aaaattgttt gttcactcga ttgagataac
    26221 ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa tggaagattt tccgcaatca
    26281 acgctttcgc ttcttgccct aaatcattca taaatgcaaa aaatacttca taaatcagcc
    26341 cttccttgct gccaaaatat ttaaaaatcg tcgctttact tacctgagcg ttctcagcta
    26401 ccttatccat tgtcactcca gcggttccta tgtctgtgtt catcaagtca aaagctgctt
    26461 gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa accgttcatt tgtagagcct
    26521 cccgtaattt taaactttta taatatatat agttcaa
//
LOCUS       DAYYDS010000032        26419 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000032 DAYYDS010000000
VERSION     DAYYDS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.032"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..2183
                     /locus_tag="ACHA02_001332"
     CDS             123..2183
                     /locus_tag="ACHA02_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8669688.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2266..2835
                     /locus_tag="ACHA02_001333"
     CDS             2266..2835
                     /locus_tag="ACHA02_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8669689.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3010..3816)
                     /locus_tag="ACHA02_001334"
     CDS             complement(3010..3816)
                     /locus_tag="ACHA02_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669690.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3954..5468
                     /locus_tag="ACHA02_001335"
     CDS             3954..5468
                     /locus_tag="ACHA02_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8669691.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5871..6854)
                     /locus_tag="ACHA02_001336"
     CDS             complement(5871..6854)
                     /locus_tag="ACHA02_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8669692.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6859..7506)
                     /locus_tag="ACHA02_001337"
     CDS             complement(6859..7506)
                     /locus_tag="ACHA02_001337"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8669693.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7591..8223)
                     /locus_tag="ACHA02_001338"
     CDS             complement(7591..8223)
                     /locus_tag="ACHA02_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8669694.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8239..8790)
                     /gene="coaC"
                     /locus_tag="ACHA02_001339"
     CDS             complement(8239..8790)
                     /gene="coaC"
                     /locus_tag="ACHA02_001339"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8669695.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8787..9524)
                     /locus_tag="ACHA02_001340"
     CDS             complement(8787..9524)
                     /locus_tag="ACHA02_001340"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8669696.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9521..10510)
                     /locus_tag="ACHA02_001341"
     CDS             complement(9521..10510)
                     /locus_tag="ACHA02_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8669697.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10768..11310
                     /locus_tag="ACHA02_001342"
     CDS             10768..11310
                     /locus_tag="ACHA02_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8669698.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11327..12412
                     /locus_tag="ACHA02_001343"
     CDS             11327..12412
                     /locus_tag="ACHA02_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669699.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12424..13230
                     /locus_tag="ACHA02_001344"
     CDS             12424..13230
                     /locus_tag="ACHA02_001344"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8669700.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13231..14058
                     /locus_tag="ACHA02_001345"
     CDS             13231..14058
                     /locus_tag="ACHA02_001345"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8669701.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14055..15128
                     /locus_tag="ACHA02_001346"
     CDS             14055..15128
                     /locus_tag="ACHA02_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8669702.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15556..18252
                     /locus_tag="ACHA02_001347"
     CDS             15556..18252
                     /locus_tag="ACHA02_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8669703.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18317..18817
                     /locus_tag="ACHA02_001348"
     CDS             18317..18817
                     /locus_tag="ACHA02_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8669704.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18829..19323
                     /locus_tag="ACHA02_001349"
     CDS             18829..19323
                     /locus_tag="ACHA02_001349"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8669705.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19344..20153
                     /locus_tag="ACHA02_001350"
     CDS             19344..20153
                     /locus_tag="ACHA02_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8669706.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20150..20971
                     /locus_tag="ACHA02_001351"
     CDS             20150..20971
                     /locus_tag="ACHA02_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8669707.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21022..23121
                     /locus_tag="ACHA02_001352"
     CDS             21022..23121
                     /locus_tag="ACHA02_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8669708.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23118..24236
                     /locus_tag="ACHA02_001353"
     CDS             23118..24236
                     /locus_tag="ACHA02_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8669709.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24275..25153
                     /locus_tag="ACHA02_001354"
     CDS             24275..25153
                     /locus_tag="ACHA02_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8669710.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25299..25496
                     /locus_tag="ACHA02_001355"
     CDS             25299..25496
                     /locus_tag="ACHA02_001355"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8669711.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            25480..25710
                     /locus_tag="ACHA02_001356"
     CDS             25480..25710
                     /locus_tag="ACHA02_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669712.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            25955..26146
                     /locus_tag="ACHA02_001357"
     CDS             25955..26146
                     /locus_tag="ACHA02_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669713.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         8370 a         4562 c         5561 g         7926 t
ORIGIN      
        1 aaaaaatcaa aaaaaatact ctcctaatag tccatcttct tttgatttta tttatataat
       61 gaaatcatct tatgaaaatt actacttcat gtttagacaa gagtagggag gactaagaat
      121 aaatgtgttc aaaaagacgt tcaaaacaac taaataaaca cgcttgtcgt ttagataagg
      181 aacggtcgtg ttctgaaaaa cactacgtta agtttatgat gcgcacgatg ctgatcagtt
      241 tatctgtagg tgtctatatg catgtacccg ttcaggcaac gacacaaacc atcaaggtcg
      301 tacgtacctc tgtttctgag accactgatt cccctatcac tattactcat actttcactg
      361 atgcaaatgg aaaacagcta cttcctgcat tgacttccta taataaagtc agttatcctc
      421 caacgataaa ccactactgg ttaattccta acaaaacatt atggacatcg gaaaccggaa
      481 gcagtgtgcc gctttctatc ttttccgata tgactgatcc tacaaaaggt gatcagttaa
      541 aacagatcac tgatattttg aatcatgctt tgacagatat aaaggacggt ggaagcgcac
      601 aagtttctta tgtatatgaa aaagattatt cacagtttga aagaaagaaa atcgatgttc
      661 tggcagggac aaaagtttcg atatctgatc tggtagaaag gctgaagaac gcagatggaa
      721 cacaaggaga tatgtccaaa gtaatttcaa acaatgggaa tatcgtggat acgactaata
      781 caggaacatc tactatttcc ttgtcctatt ttgatccaat tgctttaaaa acaatgactg
      841 catctgctgt gatccatgtt cgtgctgatc aaacagctat caaaggaaaa gatcttcact
      901 taagaacagg agagagttac gcccctaaag atcttgttga atatgtaaca aaaagcgatg
      961 gaacttcagg aaataaagaa gaaatcaaaa tgaacgggac agtagatact acaactccgg
     1021 gagaatatcc cgttgtatta acttatatag actcgaaaac atcaaatgtg gcagaaacaa
     1081 cagctgtagt ttatgtagaa gcatctgctg cttcatcgac tgtcatcact cattttatag
     1141 atagtcaaac aaataaagaa cttcaagcag aaacacatat gagtagtgta aatcgtattc
     1201 ctgatttgcg cctacctgct gaattttctg atcgagtgcc aaatatagat ttatcgactt
     1261 atgaacaaaa tgggaaagtc acttctattt ctgattggat gcaagcaaac ggattagaca
     1321 gaaaaagtca ctggtcggag atccttgaag tacttcactc agaactgatg aataatagga
     1381 agcaatcagg aggggaaatt acctttactt atgtttatat accagacaat cgttcattag
     1441 aaggtcatga tcttattgtt catccaaacg aaaaaataaa caaagaagag ttgatccaat
     1501 cagcaacaaa ttccttaggt aaacccgtac cgttaaagga tgtatcggtt acaatcaatg
     1561 aagtaccatt ggaggatggt tatttgacca aacaaacagg tgattttacg attatgtata
     1621 gttatcatga cccgtattca ctccaagaac tccatgctca gtcactatta catgttttac
     1681 cattaaacaa acagatagat caaaaagaac aaaaaagacg agaagaaagc ttgtcagaaa
     1741 aaaaacaaca aatggctcat gcagtttctc caacagggaa agtaaaacaa acaagaagcc
     1801 tagagttagc aagaatatct agaaaaatct cagaatctaa taaaacgata aggcaagaag
     1861 ctcatttggc cccacaagtt tctggattgg aaaatgaaat tttagagcct aaaataaaac
     1921 aacaatcaga aaaagaattt gttgctaaac agtcggaaga tcagaaggaa tcagatgacc
     1981 agaaggaaac gaagaaaaaa cgcttgtcaa ctattgattc caccgatgag catctgcagc
     2041 cgcaagtacc acttcctagt ccgggaacac ccgcacctgg tggaggtatt ccttcttctg
     2101 ctttaggaga tttcatgcga ggtatggcag gaacatgggt atacgctaat cgaaatgaat
     2161 ttgacgcaga tgactggttc tgatttatat ctctatctgt tatagtcaaa taagtagctg
     2221 agtcatatgt tatttggcat aatggagacg aggagggata gaaaaatgtt agataaaacg
     2281 ttaccttacg cagaaatctg gatgacaaga ccacttcaac aaaatgttcc aatcacgcct
     2341 ttagcggaag gttttgaatt tcgattttat caaaaagaag atgaatctgc ttgggcggag
     2401 attgagactt ccgttgggga atttcaaacg acagatgaag cacttgagta ttttcaacaa
     2461 aactttgctc cttatcctga agaacttgaa agaaggatgt tttttgtaga gacaaaaaaa
     2521 ggagaaaaag tagcaacctg taccgcttgg cgaaaaaaat caagagagca agtgtatccg
     2581 gtttttcatt ggttagcagt gaaaccagat taccaaggaa aagggctcgc aaaagcattg
     2641 actgccagag tcttgcaaga atttcctacc ctccatacgg acggaccaat ctatcttcac
     2701 acacagacat ggagccatca ggcgattgct ctttataaaa aaatgggttt tacttttatt
     2761 gctgaaaatc tagacggcac acctaatcca gattatgaaa aagttatgca ggttttaagt
     2821 aaattagata aataacgaaa tggtgaaata agtcctcagt attgggataa aaaacgaaat
     2881 agtcattcct catatcttac ttttttgagt taatactatc ggataactaa tggcatgcca
     2941 tacattcaaa aaagaagggg cagtgacaaa agtattgtca ctgccccttc tcttgttttt
     3001 tagcatgttt tatcgtttga tttttttagg aataaaattc ttgatgaacg taaatttttg
     3061 gtaatcgttt cgactataga ggtaaccaaa tacactgaaa acaactgctc caataaaatt
     3121 aacaaataaa tccttcatag tatccatgat tcccacgtct aagtaaccat tttggatgat
     3181 tgttttatct gttttaccat ctgcattttg actttcaatg attgtccgag tgatatctgg
     3241 aacatgatag acagtgtttc cgtctttact taactccaca ctattgattt gctggatgat
     3301 cgtatctttt tgcatatcga attgcagtaa tttatctgct gaaaattcca caaactccca
     3361 cataacacca actgtcatcg aaaaacagaa ggaaacaact gctagtaata aaggagtcaa
     3421 gttcatggtt tttgtatttt tattgagcaa ataaacaagg gaaaagccga cacccccagc
     3481 taaaaatccg ctgaatgcat gtagaaaagt atcccacatt gaaaaatagc cataaaaatc
     3541 acttaactct cctaaaactg ttgaactaaa aataaaagat aacaaaatga tttctagcat
     3601 atttggaatc tgtatcttta atatcttttc cagaaaataa gggatttcaa ataaaattaa
     3661 tgtcagtaat aaaagcattg tatttcccca gtgttggttc cagacctgat ccactagcat
     3721 aataacgacg atgatacgta ataaaatata gattccgttt gtgattctac ctgcagtttt
     3781 tgtactgttc gtaaaaaagg ttttcaaatg attcataaaa taactccttc atgtatatcc
     3841 ttaattatac taagaagcgt gtgtttacca aagctatatg acttaaagaa aaacagagtt
     3901 gatattgtga aaaaaatcac ttaatatagt ataaggagaa aagggggata caaatgaaaa
     3961 aattagttgc tggtttatta acagccgttg cacttcttgc tatgagcgca tgcggcgcag
     4021 atcagaaaaa tacagcgcag acaagttcta gtacgaagga atctgccgaa gtatcatcag
     4081 gcgcttctga acaagtatat acagatccaa aagaaatgaa agaatcttat gatgtcgtga
     4141 ttattggagc tggtggtgca gggatggcag cagctatcga agcaaaagat gctgggttga
     4201 atccagtaat tttggaaaag atgcctgttg ccggaggaaa cacatctaaa tcatcaagcg
     4261 gtatgaatgc ttcagaaaca aaatttcaaa aagaagaagg aatcaaagac tcgaatgatg
     4321 ccttctatga agaaacgcta aaaggtggtg gaggaacgaa tgataaagaa atgcttcgat
     4381 actttgtcga tcactctgct gatgcggtcg attggctaga tcaaaacggt atcaaactgg
     4441 acaacttaac gatcactgga gggatgagtg aaaagaggac acatcgtccg tctgatggct
     4501 cggctattgg tggatactta gtggaggggt tattgaataa cgtccatgag cgggaaatcc
     4561 ctatttttgt caacgcagat gtgacagaca ttaagaaaaa tggggaaacg gtcaatgaag
     4621 tgactgtaca tgttcaagga gaagaaccaa aagaggtcac cggaaaagca atcgtagtta
     4681 ccacaggcgg ttttggtgcc agcaaagaat ttattgaaaa atatcgtccc gatttagctg
     4741 attatattac gacaaatcaa gaaggatcaa ctggagatgg tatcaccatg attgaaaaat
     4801 taggtggaca aaccgttgat atggataaaa tacaaattca tccaactgtg cagcaggatg
     4861 agggggtctt gattggtgag gttgttcgcg gagaaggagc aatcttggtc gatcaagagg
     4921 ggaaaagatt tgtcaatgaa atggatacaa gggataaagt atctgcagca attacagcat
     4981 tgcctgaaaa atcagcttat ttgatttttg accaaggcgt gcgtgatcgt gccacagcta
     5041 ttgaatttta tgaccaaaaa ggatatgtca caactggaaa gacaatggaa gaattagcag
     5101 aaaaaatcga tttaccaaaa gaaacactag caaaaacagt tgatggatgg aatacggatg
     5161 ttgcggagaa aaaagatgct caattcaacc gtgccacagc catggaacat gacttatcgt
     5221 ctccgaatta ttatgcgatc aaaatcgcac caggaatcca ttacacgatg ggcggtgtga
     5281 aaatcaatac gaacactgaa gtattagata aagaaaacca gccaattagg gggttatatg
     5341 ctgctggtga agtcacaggt ggcttacatg gaaacaaccg gatcggtgga aattcagttg
     5401 cggagatcat tatttttggg cgacaagcgg gcatacaagc agctcgattt gcttcggaaa
     5461 atgaatgaaa tagaacgtaa ataaaaagag gttgtgaaat cagcgttttg agctaatacc
     5521 gagggtcagc tgattgaaca ctgtttagtg aagaggttgt gaaaaagctg ccctgcatca
     5581 agtaataaga acagccaagt aaaaacagct tcttatgttt ttaacttggt tggacttatt
     5641 accgcagatg caagcttttg aacaccgttt attcggaaaa agagcgtggg acaaaagtaa
     5701 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gaaattcctt
     5761 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
     5821 tcttggagtt aaacgcttct gtcccagcct cttttttgat cagcatgatt ttattcagtt
     5881 atcttcaaaa tgatttttcc gatgtttttg tttgcttcca tatattcatg cgcttgtttg
     5941 actgcgctga attggaatgt acggtcaatg attggccgaa tcttgccatt ttctagaaaa
     6001 ggcccagctt tttgcataaa agtttgtgcg agctctgctt tatactgatc acttctaggt
     6061 gtcaataatg taccaagcag ttggacacgt ttcccaatca atacaccaag gtctatctca
     6121 ggcacgatgg tcccacctag cataccaatc aagacccaac gtccatcaac ggctatagaa
     6181 gctaaattct tttcccagta agaagcgccg atgaaatcca aaataacatc gactccccga
     6241 ccttcagtga tttttcgtac ctcttcttga aagtcttgtt ttttatagtt gatcaattca
     6301 tctgcaccaa gttctttaca aaaggcgagt ttttcttctg atcctgcagt aacgatgatt
     6361 ttcgcgtgag aaagctgtct agctaattgg atcgctgctg ttcccacacc acttgctcct
     6421 gcatggatca agacagtttc gccttcttgt aattttccaa gccagtaaag tgtttgataa
     6481 gcagtcaaaa aaacttctga gattccagca gcagaaacat agtctagcgt atcagggaga
     6541 aggattgcac gatcggcagg catcacagca tattcggcat aaccacctag attgactaat
     6601 ccgtatactt ttgtcccagg agtaaaagtg gagatatcac ttttattctc aactacctct
     6661 ccagctactt cgactcccaa aatagggtag ggaggtttca aagagactga ttctcttttc
     6721 ataatatctg ttcgattgac agctgctgta tggactttga tcagtaattc gccttgtttt
     6781 ggtttaggaa caggcacttc aatttgttct aatacatcta cagaaccagg atgcttgatt
     6841 gaaatggcac gcatggatct attcctcctt tgcccattct tggaatgctg tattcaactc
     6901 tttgctacct tcagaaagta ctagagtcgt acgagaaata tccgtttctt ccactagagc
     6961 ggcaagtaca gcagcaactg tagagcgagt gatcgttttt gatgccttct cattaggctt
     7021 agttaattga atggaagtaa cttcttcatc gttagtcaat gtgactggtt gaagaatgac
     7081 aaaatctaag ttagttcgtt tcagccattc atctgcataa tgtttagtaa tataataatc
     7141 tgtcatggac tcaggccatt ttgctggttc atctgcataa acagcactga ccatcagata
     7201 gcgagagaca tgcgcatttt cagctgcgat cactgttttg attgcgccat ccaagtctac
     7261 ttgcagaaga ttctttcctt gagagccggc agcaaaaatc aatacatcga tgcctttgaa
     7321 tgcttcggtc attttttcca cggaccaagt taaatcaaaa gagacatacg aaacattctc
     7381 tttttttact aatgtttggg aagcaacatc tctcacccct gcaaaaattt catgggaact
     7441 tggagctaaa tcttttatca ggtgtcggcc aatttgtccg tttgcaccta caacaaaaat
     7501 tttcatttgg atcatccttt cgacgtcaaa tctttccatt caatagtaac accaaaagag
     7561 aaaaaaatca gagatcggct acgatgacta tcagttagta agagaagggt gcttgaagta
     7621 tcttaacaag acattgcata ctgctgcagt cgcaagacct gcgacgattc cttcaggtat
     7681 accacttgta aaaataaccg ctagaatagc tgaataaaca gcatttaaat ttgttccgat
     7741 gactttggca taatcttgct ggaataaaaa gtagatcata ttcatcacta aaatcgtatt
     7801 ggtcaatgaa ccggaaagtc cagctaagaa taaagaaacg gaatctgctt tttgtttcat
     7861 tagtttgcgc agacctttat aaacgaaata aggaaccacc ccaatcaaga tgcgaggaat
     7921 aaacgcgatc agcaaggctt tccagcttcc atgttctgtt ccgatgactg ggataaacgg
     7981 agaaaagaca aaagacaaag gtgtttggat aactgtgcta cggatcatgc tgatcagtcc
     8041 aaatgtaccg ccaagaaaag cacctagtct tggtcctaaa acgatcgaag caatgattac
     8101 aggaatatgc atggttgttg cattgattgg accgataggg ataaaaccta aaggcgtaac
     8161 agctaataaa atcaaaatag ctaaaaacat cgcagttaat gtaaagtttt ttgtattttt
     8221 cataagtact ccttttttct aagcatttct ttcattaaga gtcagcatga cctgttcgat
     8281 gatcgtctga atatcagcta aggcaccttt tccaaaatct ccacaagcta aaagagattc
     8341 tcttggttca atttcctgat agccgacaga tctcaatgtt tccagattac gctgagtgat
     8401 cgggttttga tacatattcg tgttcatggc aggggcaatc aattttggtg ttcccactgg
     8461 aagcgccatt gcgactgttg aaagaagatc gtctgcaatg ccgtttgcta gcttgccgat
     8521 aatgtttgct gtagcaggcg cgatgagaaa caagtcagct tgttttgcta gttcgatatg
     8581 attgattacc tgcggatttg attcttgcat gacgtctgta tggactgcgc gcttcgacag
     8641 agattgcaag gttaatggcg tgataaaatg tgtgctgctt ttagacataa tcacatctac
     8701 ctgggcacct aatttgacga attgacttgt aagatcagcg cttttgtaag cagaaatact
     8761 tccagtaacg cctagtaaga tctttttcat tttgattcct ccagtctaat tttctcagta
     8821 atcaatgcag caatctcaga tttactttga gcttcttcca ctgttccatc tttagataag
     8881 agatagccgt ggtgtgtctc gccatggatt tccgtcaaat catttgcgaa aataaaatca
     8941 gttttgttat ttatcagact attttttgcg actgccagaa gagattcctt tggtacatct
     9001 actaatagtt tgaaaccaat gatttttgct tttggctgcc attcacgtag ataagaaatg
     9061 atttttggtg ttttcttcaa aacgagcaca agatgctcgg tgttagaaga gatctttgaa
     9121 acagtggtat ctgtctgttc attttccgca aaccaattgt ccagttcttc ttgcgttgaa
     9181 gaagtgggta ggttttttgc cagctgttct tctgaaaaac taaaagcagg agtaaaatca
     9241 ctgactgcca tactgtggat aatcgcatcg tagtgttctt ttttcgttag ttcttctaat
     9301 tgtaaaaata agtccagtgc agattcgatc gtataaatcg ttatattgga gtgtcttttc
     9361 ggtttcagtg cttgtttcgt agtcacgtaa tcgattgtgg tatccggatt ggcagctaaa
     9421 taatcagcta atgcttgccc taatcgtcct gtcgaatgat ttgtaatagc acgtacttga
     9481 tcgatttttt cagtcgttcc tccagctgtg attagaatct tcatttatgg tcacctacat
     9541 ttactagtgt tcttccttgg tgtgttccag caagtagttt agatgctgtt tcaggcaatt
     9601 gatccagagt aatctcttgg acgaccagtt catctgcaat ctgcaaatcc gccatctgtt
     9661 gccataaaga aagacgttga tctattggta cattgacaga atcaattcca atcaactgga
     9721 tgtttcgcag aataaaaggc aagacagtcg cgtttagttt gattccccca gcattgccac
     9781 ataaagctac tgcaccgtta taagagatca atggaagaag actgcttagc tgctcgccac
     9841 ctactgtgtc gatgacatag tcgatttgct gttttcctaa agcttttgtt gtctctggca
     9901 gaaattcatc aggtcgtacg acttgcaaag cacctagact ttttagccat gtgacagact
     9961 cttcttttct ggaaaaggca atgattgact ggaatcctag tttatgtaac agggctatcg
    10021 ctgtactgcc tacaccgcca gaagcaccag taacgacaac ttttttatcg gaggtcatac
    10081 cttgacggat taaagcgttc acacacaaca tagcagtaaa ccctgctgta ccaaggatca
    10141 tcgcttgacg cagcgtcata ttttttggta gaggaactaa ccagtctcct ggtacttttt
    10201 gatattggct ataaccgcct ggatgggtga cgccaagccc aaaaccagta gcaagtacaa
    10261 gatcttcttt tgaaaaacgt ggattgttac tttggacgac agttccggat aaatcaatcc
    10321 ctggagtcat cggatatgtt cgtatcacct gaccagactc gctcattgcc aatgcatctt
    10381 tataattgac atcagaatag gcaattttta ccaagacttc attttcagcc aactcagtaa
    10441 atggcagatc actgattgct gtatggaaaa caggttcctt tattacctga aaagatcgaa
    10501 aagcgttcat tcgtgagaca ctccttttaa aattttcttt tatcatacca taaagcctac
    10561 tcttttattc ttgttcttcc gtaaatttag acaaaaatta ggtgaaaaat aaagaatcaa
    10621 acaactagaa tttattccaa aagataaaga aatattcagc aaaaaaaatt ttacaaaggg
    10681 ttgccttttt caataaattt cgtatactaa tacgagtggt agtaaacagg aagtttacta
    10741 ttagaaaact tcgattcgga ggaaattatg gaaattggtg agaagttaag aaatttgcgc
    10801 atccaaaaga atttgacaca agaagaacta ggcgaacgca cagatttgtc aaaagggtat
    10861 atttcccagt tggaacgaga tttaagttca ccatcaatgg aaacattctt ttctattctt
    10921 gaagtcttag gtgtgactcc agaagaattt tttcatcaag aagcagcgaa tctgcaagtg
    10981 gtttattcaa aagaagatca tacggtttat ttcgatgaag aaaacgggta tgaattggaa
    11041 tggctcgtgg ctgattcgaa tgaaaaagaa atggagcctg tcttgctgac ttttgaaggt
    11101 gcaggagaat ataagacgtt tgaaccttca ttatctgaaa cgtttatttt tgtattagaa
    11161 ggggaattag agttaagctt aggagagacg gtctatgtgg caaaagcagg acagtcgatc
    11221 tattatcaag cgacaaaaca acatcaatta aggaatcatc tcaaaaaacg aacaaaagta
    11281 ttgattgttg caacgcaatc atatttgtaa tatagggggt aaaaacgtgg ggaaaccaat
    11341 catttcattt gatcaagtag tgaaacgcta tgatgatgaa acagtattaa aaaaagtaag
    11401 ttttgagatc gaacaaggaa aattttatac actgcttggt ccttccggct gtggtaaaac
    11461 aacgatccta cgtattatag ctggattcac cgaagctaca gaaggggaca tctattttga
    11521 agggaaacgg ttgaatgatt tgccagctaa taagcgacaa gtaaatacag tcttccaaga
    11581 ttatgcactt tttcctcata tgaatgtttt tgataacgtt gcttttggat tgaagatcaa
    11641 gaaaatgtcc aaatcagata tcgaaaagaa ggtaaaagat gctttgcgta tggtccagct
    11701 tcctggatac gaaaaacggg agattagtga aatgtctggt ggtcaaagac agcgtgtagc
    11761 gattgcacga gcaattgtaa atgaaccgaa agtattgcta ttagatgaac cgttatcggc
    11821 gcttgatttg aaactacgta cagatatgca gtatgaactg cgggatctgc aacaacgatt
    11881 aggaatcacg tttatttttg ttactcatga ccaagaagaa gcgttggcaa tgagtgatga
    11941 gatttttgtc atgaataaag ggaaaatcgt ccaaagtggt acacccgtag atatttatga
    12001 tgagccaatc aatcatttcg ttgctgattt tgttggggag agcaatatcg tggacggtgt
    12061 aatgatcgaa gataatctag ttgagtttgt tggtaagcaa tttgaatgtg tcgatggcgg
    12121 gatgcgtcta aatgaaccag ttgaagttgt tttacgccca gaggatttga cgatcacgac
    12181 accagataaa ggaaaactag tcgtaacggt cgatactcag ctgtttcgtg gtgtccatta
    12241 tgaaatcatc tgttatgatg aacagcaaaa tgaatggatg gtccattcaa cgaaaaaagc
    12301 taaagaagga agcaaagtag gattggcctt tgagccagaa gatatccatg tcatgcgttt
    12361 caacgagtct gaagaagaat tcgatgcacg tcttgacagc tacgaagaat aggggggaca
    12421 agaatgaaaa caatgcgcag gatctattcc gtcccttata ttttgtggct cggtttattc
    12481 gtgatcgcgc ctgtattaat gatcatttat caatcttttt ttgatatgaa tgggcaattc
    12541 acgctgaata attatctatc ttattttacg tcggggacat cgctttctat gacgatcaat
    12601 tctgtatggt atgcattttt gatcacgttt ttcacgctga tcatcagtta cccgactgct
    12661 tattttttaa caaaactgaa gcacaagcaa ttatggctga tgctgattat tttgcctaca
    12721 tgggtgaatc tgctgcttaa agcttacgca tttattggga ttttcagtgt gcacggcagc
    12781 gtgaatcatt tctttgattt tatcgggatc gggccgaaac agatattatt cacagacttc
    12841 agttttctgt tcgtggcaac gtatatcgag attccattta tgattatgcc aattttcaat
    12901 gcattggaag agatgaatcc ttctttgatc agtgctagta gagatttagg cgcgaatagc
    12961 tttgagacct tccgacgagt gatttttccg ttatcattaa atggtgtgaa aagtggtgtc
    13021 caagcagtat ttattccttc tctttcttta ttcatgctga ctcgtctgat tggcggaaat
    13081 cgggtgatca ctttaggaac ggctatcgaa cagcatttct tggttacaca aaactggggg
    13141 atgggttcta caatcggtgt gattttaatc attgctatgt tcatcgtgat gctcctgaca
    13201 ggcgagaaga aaaaaggagg tcgctcataa atgaaaaaat ttaaatggtc gtatctttat
    13261 ttagggatcg tctttttatt cttatatgca ccgatcttct atctgatttt ttattccttc
    13321 aatgaaggag gaacgatgaa cagctttaca ggatttactt gggaaaatta tgaagctgtt
    13381 tttgaagata cacgtttgat cattatcgta ttgaatacgt tcatgctggc tttcttatct
    13441 gcattgatcg ccacgatcat cgggacattt ggggctatgg gaatctatta cacgaaaaaa
    13501 cgacgtgcac ggacagcgtt gttgagcatg aataacatct tattagtctc tcctgatgtt
    13561 attatcggtg ctagtttctt gattttcttt acactactag gattcggatt aggttttcag
    13621 agtgtattgc tttcccatat tgctttcagc attccaatcg tcgtcttgat ggtcttgcca
    13681 aaattgcagg aaatgaacga ttcaatggtc gatgcggcta gagaccttgg tgctaataat
    13741 ttccaagtag tcaaaaatat cattttgcct tatctttctc caggaatcat agctggatat
    13801 tttatggcat ttacctattc tcttgatgat tttgcggtta catttttcgt cacaggaaat
    13861 ggctttacga cgctctcagt agagatctat tctcgtgcaa gacaaggaat cagtttagaa
    13921 atcaatgcgc taagtgcgtt agttttcttg ttctccatga tattggtcat tggatactac
    13981 tttattagta aagaaaaccg tccaaaacgg ctgaagaaaa acagaagagt acgcgagggg
    14041 gtggcacggc tgcgatgaaa aaattacaat ctctgttttt aggtattctc atcattattg
    14101 cggtgctgct gcttggtgtt cgtcaacttg aaaaaacaag tggcatgagt ggagcaaagg
    14161 tacttaccat ctataactgg ggagattata tcgatcctag tttgatcacg aaatttgaaa
    14221 aagaatatgg atacaaagtg aattatgaaa catttgattc taatgaagcg atgttcacaa
    14281 aaatccagca aggtgggaca aactacgaca tcgctatacc aagtgaatat atgatccaaa
    14341 aaatgatcaa agaaaaacta gtacttccat tagatcattc gaagatcaaa ggattaaata
    14401 atattgatcc acgattttta gacttggatt ttgatccaga taatcagtat tcgattccat
    14461 atttttgggg aactttaggg atcgtatata acgataaatt ctttgatgca aatcaaatta
    14521 agcattggga tgatttgtgg aagccggaat taaaagacag tttgatgctg attgacggtg
    14581 cacgagaagt tatggggctt tctttgaata gtttaggcta ttctttgaat agtaagaata
    14641 tggcacagtt gacagaagca gcaaataaat tgaatcattt aacaccaaat gtcaaagcaa
    14701 tcgtggcaga tgagatcaaa atgtatatga tcaatgaaga agcctccgtg gcagtgacat
    14761 tttctggcga agctgccgat atgatgtggg aaaatgaaca tttacattat gtgatcccac
    14821 cagaaggatc caatctttgg tttgataata tcgttatgcc aaaaaccgtg aaaaataaag
    14881 aaggtgccta tgatttcatc aactttatgc tagaaccgga aaatgcggca caaaatgcag
    14941 agtatattgg ttattctaca ccaaatgaag aagcaaaagc gttattgcca aaagacattt
    15001 cgtcggacga acaattctat ccatctgatg acacgatctc ccatcttgaa gtctacaaag
    15061 atctaggatc gaaataccta ggcatctata atgacctatt cttagaattc aagatgtata
    15121 ggagataatg gcaaaactca gtaagaatgg ggacttatca acgttctaaa gagctaaaaa
    15181 tcgtcctttt tctatctgag ttttccaatc ttctaccaaa agcggatgaa acgccgcttt
    15241 ttttgtgtta ttttattttt tctaccacgt ttttctacca gagaaaagtg gacaaaacca
    15301 ttgtaaaata gggcccgaga cgggctagta aatctaagat tgaaaaagtt acagaaggat
    15361 aaagtaattg gaaactatgc aaatatactt aacccttctg taatctatta ataaaactaa
    15421 tatatgaatc cagttcaata tttgtagcta tgaaaaaaca taaaaatcat attagataaa
    15481 aactaaattg gtagataaat tattttgtca gatatctgag gcaaatattc gactatttgt
    15541 gtttcaaagt aaaaattgga atacaaatat agaatagaca tattgcagcc gatagtttat
    15601 tatgctttta gacacattaa actggaggaa aaaatgaaaa agagcgctaa agatcgagtt
    15661 tttgatgagc tgatacgact aacagggaat atgtcttctg tcactgccga gaaaatttcg
    15721 aagaatctag gtatctctcg tcagaatgcc agccattatt tgacgagatt agtggaagat
    15781 aaaaaggtcg agaaattacc gggaaagcct gttttgtgga agcctttaga tgaatatgcg
    15841 gttgtagata atacggagca aataaacgaa gcctttcatt ctgtagttgg tcatgacgga
    15901 agtctgagag aggtcattca aaaatgtata gccgctgtca aatatccgcc taacggactc
    15961 agtgtattaa taaatggagc cacgggtgtt ggaaaaagtt ttttagccac aaaaattttt
    16021 gaatacgcta tccatgagca aattattgaa aaagatgcac catttgctat cttaaactgt
    16081 gccgattatg cggacaatcc cgaactgctt tcggcaacac tgtttggata caagaagggg
    16141 gcctttacgg gggctgaaaa ggataccgat ggtttattgg caacggccaa caacggctat
    16201 ctttttctcg atgaggtcca tcgcctatcg aaagaaaatc aagaaaagct ctttctattt
    16261 attgatactg gaaactatcg tccagtcggt gaaaatgtaa attggcattc tgctaaggtt
    16321 cgttttattt tcgccacgac ggaaaaaggt gaaaattatt tattagacac ctttgatcgg
    16381 cgtattcaaa ttagtgttat gttgccaact tttgaagatc gccccattcg agaacgatta
    16441 gagctgattc aattattttt tcaaaatgaa gcggatgttt tacaaaaaga tattattgtt
    16501 tcaagagaag ccttgtcttt aatgttatct catcccttta gtgggaatat tggtaagctg
    16561 aagaatatca ttaaaatcag ttgtgcggat gtgtatagtc gaacacaaga agaaccatta
    16621 acgattggga aaaatgagat cttgattcag ttaaatatgc tggaatccga agtagaaagc
    16681 ttgccgattg ctagtttatt gataaagaaa aatcaaaaga taaaaacgga tcgaatcaaa
    16741 ccgtttgatt atgattcgat tttggaaaaa attactcagc tgataaatga aggtaatttc
    16801 aatgaaaatt tttcttcgat aaagaatgaa gtacagcatt tggtgcatca cattgataag
    16861 gattcttttt tatcaatgag tagaaaattg atgattcaga tgtttagaaa ggtctgggta
    16921 tccgttttag gaaaaacgta cggattgacg acgagtggac ctgttgcaga aattattagc
    16981 agatcctatg tcatttcccc aagctataag acagacttaa ccgggataaa tcgtacatta
    17041 gctgcaaatc tacctcggac gacttactta tgtaatcagt tcttggagca tttaccaccg
    17101 ctctcagaaa aggacgagaa ctttctaaga ctgcttattg tcgtgacttt atctgattat
    17161 gtagatgaat ccatcgagct gaagggcctg ttgcttgctc acggagatag tacggctagc
    17221 agcattcaag cagtagtgaa tcaattatgc ggcaattatg tttttgaagc gttggatatg
    17281 ccagtagaga gtaatgtgtc agagattgtc gaatcgacaa ggaatttcgt gaaacggcaa
    17341 gtgaatacag atagtctagt gctgatcgta gatatgggct ctttaaatca aatttacaca
    17401 cagatcaaga acgatttaaa aggggagtta cttcttctga ataatttgac gacttccatt
    17461 gcacttgata ttggcttgaa gatgacgatg aatgcgccat ttaaacagat tgcggaggat
    17521 gcagcagaca agtattcaat caatgttcaa tactttgaag gcttttctca aagtagcaac
    17581 attatcattt cctgtatgtc gggtttgggg atttcggaca aattgaaaga tgtttttcgt
    17641 catcatttat cggatgaatc catcgaagtt ttcacgaagg actacagaga actacgtgaa
    17701 ttaatcgacc aaaatgatga aacttatttt gacaagacaa aattggtcat cacgacttcg
    17761 gatcttccac gttccttttc aattcctaat attaatgttt atgacatctt agacggtgat
    17821 ggagcagcca atttggagca tattttgagt cctgaaatga agccggcaga atttaaccgc
    17881 ttgattcagg agctagtaaa attcttttcg ttggaaggaa ttgcggaccg tttgaacttt
    17941 ctgaacccaa cgatcgtgat gagtgaagtg gaaacagtca tttctaaata tgaaaattac
    18001 tatcatttca ctttaaatgg ccgtgtaaaa ctaaatctct atatgcatac ggcattgatg
    18061 atggaacgtt tattcttggc tcggacagaa aaatctgaag aaccgaatta tgatttgaaa
    18121 ccagatgaag atgaatttta ttatgtttct agaagtattt ttcaatcaat ggagatgaaa
    18181 tataatttta aagtcaatgc ttatgagctt tcgctgctct atgaattatt aggaccgtat
    18241 attcaaaaat aaagtgtcta aaaaagtgtc taatctgttt tttttaggtt agacactttt
    18301 tttaatgtga attaacgtga ttaaaccaat gtttttaaaa gttggcacag gaattgctta
    18361 tataaaagtg aacagaaaga aaggaattaa ccatatgaag aaaattctaa tagcgactca
    18421 cggtcactta gcttcaggct ttcttagcag tattcaatta ctgacgggga aggttcaaga
    18481 gattacggtg attaatgctt atgtggatga gtgtgatttt gaacaggagt tgaatgtatt
    18541 tgtaaaccag gtgaacgagc aggatcagat ttttgttttt accgatttat ttggcggaag
    18601 tgtcaatcag aagattacca aaaccttttt ggagaaagag attgctgtaa ctattttgtc
    18661 tggctttaat ctgccaattc tgctcgaaat agtattggca accaaggaat taacaaagga
    18721 ggatattcag gaactggtaa ataaatgcca acaggaatta aagatgacca ttcttgatga
    18781 ggtacatgta gtaaatgaag aggaagattt tttttaggag gaagaaaaat gattaaagca
    18841 gtgcgaattg atgaacgatt aattcatgga caagtagcga tgacttggac aaagagtcta
    18901 aatttaaccg ggttagtagt agcaagcgat gaggcggcta ccaacgatat tcaaaagatg
    18961 acattgaaga tggctgcacc atcgaatgtc aaagtcatta ttaaaacagt tgatgacgcg
    19021 attcatttgt taaaagatcc gcgggcgaag gacatgcgct tgatggtttt agtccctaat
    19081 gtcaaagatg cggtaaaagt tgcgaaagct ttcccaacgg aaatcgaatt aatgaacgtg
    19141 gggaatgctg ggaaaatgac accgccatcg gaagataaga aaaccttatc aaaggaagtg
    19201 atgttaacga cgtcagaaat tcaagactta aaagaattag ttgaacttta tccagatacc
    19261 gtttttcagc ctacaccggc gatggaaaaa cgttcagcta tctctatttt gagtgatttt
    19321 tagaaaaagg aaggagaaaa attatgtatg atgcatttgt agtcgctttg gccgttttta
    19381 ttggaattgc tggacatgaa atgttcggga tgtccatgct gagtcgtccg attgttgttg
    19441 ctccgttagc aggagcgttg atgggggatt tgcagacggg gttgattgtc ggggcgtcac
    19501 tagaagccat ctttatgggt gtcgtcaata tcggaatctc tagtacggcg gagccttctt
    19561 tggctgctgg gctggctaca gcgttcgcaa tcaaaatggg aggaaacgtg gatgcgatta
    19621 ttcccattgt ctttccacta gcagtattag gattacagtt aatgaatatg attttttctt
    19681 ttgtatgtgg accgatggct gctaaatttt tcaaatttgc aaaatctggt gatcaaaaag
    19741 gaatcatcaa tctgcacttc tttatgtggt ttgtccacta tgcgctttat gctcttattc
    19801 cattctttgc cgttttattt ggagcagatg ttgtcaaaag cgtcttggat aatattccag
    19861 aggtcatcat gaacggatta acagtcgctg gaaatctgct gccggcagtt gggatggcga
    19921 tgctgttacg gatgctttgg agtaaaaata ttgctgtgtg gtttttcttt ggggccgttg
    19981 ttatggcgta tctcgagtta ccattgattg caattgccgc agttggaacg attattgcaa
    20041 ttgttatagc ccaaagagat ctacaactaa aaaatatatc tgttcatgca gttcaaagcg
    20101 atgctgctgt aaatgcaacg cttgaccaag aagaggagga tttctttcaa tgaaattagc
    20161 tgatggaatt tctaaagatg agaaaaaatt ggttcggaag atgttttggc gctcgatgac
    20221 tatgtatgct tcggtgaatc cggtgacgat gggcggcggt ggtttcgcct attctatgat
    20281 gcccttcatt aatcatttct acaaaagtga agaagatcga gctgcggcgc tggaacgtca
    20341 tacacagtat ttttcgacga ccattccttg cgcgtccttt gtcatgggga ttgcggcatc
    20401 gatggagaag gaaaattctg aaaaagctgg ttttgacgtg gaaagtatca acgcgatcaa
    20461 aacgagtttg atgggaccat tggctggaat tggcgattca ttgttctggg gagtttggcg
    20521 tgtcgttacc gcgggactag cgatcaactt agccaatgcc ggaaatattt tggcaccaat
    20581 tatcttttta gttatgttca atatacctaa ttacttgatg cgatactatg gcggcgtttt
    20641 aggctattca ttaggctcta aatatattga aaagctgtat gcgaacgggt taattgaaat
    20701 tctgacaaaa gctgctagta tcttgggtct gatcatggta ggtgcaatga cgatcagtat
    20761 ggtcaaattc aataccgttt tgagcttttc aatgaagggc gaagaactaa tgaatgtcca
    20821 agaaatcctc gatagtattt ttaaaggaat tgtaccgtta ttggtaacat tgagctgttt
    20881 caaattacta gataaaaaag tcggcgtcat caaaattatt tttggaatgg tcgtcttggg
    20941 aattttattg tcattacttg gaatcgcata gtaggaagct gcagctacta ggctgcagtt
    21001 tttttagcaa ggagggaaaa gatgactatt aatgaagtaa tttattcacc caaacaccca
    21061 ttttttaatg ctgagttaaa cgacggaaat gtgactactt caaacgaata cagctactat
    21121 cggaatattc ctgatgtaaa gggattgacc ttcgatgtaa gtaatcaaaa gacattagca
    21181 aaccttgatg tttgcgggac gattcaaacg ttgacctttt atcgagaaaa tttactaact
    21241 gaggaaaaac caggtgtttg ggtcaataaa cagctctctc aaaccaattc gttatcctta
    21301 aaggttgaag taaacgggat tgtctaccat ttgtcagaat cggatcatcg tattgaagtc
    21361 gatgtgattc aagactgttt gcctagatat attcatcatt attcgacgtt taaggtcatt
    21421 gtagtatcct ttgctcccat actggctaat aagcgtttaa gtatgcttgt tcaacaggtt
    21481 tacattgtga atgaaacaca cgaaagcctt caagtttcag tgaaagatgt gccttcgtat
    21541 caagagaagt attcggatca gcaaaatgtg ttgatttctc aaaaaggaaa taaggacacg
    21601 atcgcacaac aggaagtggc aagcttcgcc attgcttttg tggacccaaa tgcttttgaa
    21661 gaagtaatga cttttcagga atctgatatc gaaaaatggc tgaaggaaac attgaactat
    21721 tttgctcagc tatttgggca gctgtcaatg ccaaacgata gagttgtcca tctctatcgt
    21781 cgtgcacttt atcaaagctt ttctagtttc ggtatgaatc gacagggaca ggtcgtcggc
    21841 agcaattggg gcagctatcc tgctactaat cgaatttgga ataaggatat gtattacgca
    21901 tcattgccat tcaccatgtt tgaaccggag ttatgtcaaa aaacggttct atggtttgat
    21961 caatatggcg ttaagtttcc tggttcaaaa tttacaggag gtatcaatca ttctctgagt
    22021 aattcgttat ccagtgcgtt actggctagt ctttattttg aacatactgg ggatacaaaa
    22081 ttctttgagc ggtgtccgca ggttctactg aatgcttcaa gaattattga ggacatatta
    22141 gctcaacgtc ggtcagggga accaatgctt tttccatctg tttggttatc agacgccttt
    22201 gcacttggaa agtatcatac cggcagcaat ctttgtcttt ggaaggcctg tattggactt
    22261 gcagaaattt ttgaagccct agaacaactg agtcttagca agcgatacaa aaacattgcc
    22321 tgtaaactaa aggaatcaat ttgtaaacaa atgactatcg atggtccttt tggtgagcaa
    22381 tttttggagg gaatcggtga tgaggagaaa acaacctatt cagtgaagca ctatcaaaaa
    22441 ccgattcttg agcaagggtt aatcttttta agtgatgtga ttgtggatgg aaaaattaat
    22501 ttattgatgc atgatggaga agaatcagac actacgttga tgccttttta tcaattttta
    22561 gataataaag atcatttata tcagaacacc atggcctttt ctgctagttc tttcaatcca
    22621 acctatagcg aggaaatcaa aggcatcact tggggattag aatccggcgc cacttttcct
    22681 ggctttatca ctgttttaat gagcgagctg cacaaccctc aagctttcgt aacccgatta
    22741 gaagaactaa tttgtttggc agatttagac ggttcgtggt ggtggtggcc gtatcgctta
    22801 gaggctcaac aaggagaagt cgtgcgagac tttggctgcg gcaaatgcgg ctgggcatcg
    22861 ggtctttttg tcagcctaat gatcagtcaa tatttcggat tgaaattcaa aaaaggagta
    22921 ttgcaaattg atcctgttga taatttaacg ttttcttgga agaatttgaa gttcggtcaa
    22981 gccttcattt cgattgaatg tacgcaatcc gagttgagta tttcaaattt gtcagaaaag
    23041 ccattgaaaa taagtgatac gaaaaaaatt cttcacgtag aaaagggaaa aacgattcac
    23101 attcaaagga gaaaaagatg agtatactaa aaaatattac ccaatttaaa aaaatccaga
    23161 gtcgagcaat cactcccgaa aatccaacag gcgagaaagg taaagctgcg atgttgccaa
    23221 gcgagctagg accaagccgc aaaggtcgtg gcgcaattcc gctgacttct ggtgaagaaa
    23281 ctgtgattgc ggatattact ggaacgggaa taattcgtca tatgtggatg acgattcgtg
    23341 atcgaacaga atttggcagt tttgttttac gtgatttaat cttaagaatt tattgggatg
    23401 ggtcggatag ccctgctgtt gaaagtcctt taggggattt cttttgtaat gggtttggag
    23461 agcgttgcga ggtgaactcg ttgccgatag tggttaatcc tacagggggc tttaattcct
    23521 atttcgaaat gcctttccaa acatccgcta aaattacgat cgagaattta catccgaagg
    23581 atatgtcctc atttttttat acgataaatt acgctttggt ggacgaattg gaagaaaata
    23641 gcctatattt tcacgcaaat tggcatcggg aacgcattac caaattacag gaggattata
    23701 agttaattga tcgattaaag ggtcaaggct attacgtggg tacttacctt gcgcttacct
    23761 gcctagaacg ttattggtgg ggcgaaggcg agtttaaatt ctacattgat ggggatagtg
    23821 agtatccgac cgtaacctca acaggttcag aagattattt tggcggggcg tgggcctttc
    23881 atgagtacga tgagttcgga cgtccacatg cgaaaaacta ttcaacagcc tttttagggt
    23941 atccttacca atcaaataga gatgccacaa gagatcgttt tgaaacggga aaattgaatg
    24001 ccgttcatgc ctttggtgac gacgcaatgc cacgacatgg tctttatcgt tggcatcttc
    24061 aagaccccat ctgttttacc gaagatttat cggtcacttt acaacaaatc ggcaacgatg
    24121 atgttcgctt atttgagcgg caagacgatg ttgctagtgt cgcttactgg tattcaacag
    24181 aagcttcagg aaatactaaa cgtcatttca ctgatcgaga acgcttgccg cgttaatgaa
    24241 gtgtttttca catattcgaa gggaggaacc tatgatgata tatggggcaa ttgaagctgg
    24301 agggactaaa tttgtctgtg cagtcggtac tgaggaatta acgattttgg aaagagtcag
    24361 ttttcccaca acaactccag ttgaaacaat gaagcaggtc attgattttt ttaaaccttt
    24421 taaggaacaa ttggcaggca tcggggtcgg ctgctttggt ccaattgata ttcaatgcaa
    24481 atcaacgagt ttcggtcata tcacatcaac accaaaactt gcttggcaaa atttcaattt
    24541 tgtaggaacg ctaaagcaat atttcaatat tccgattgaa tggacaacag atgtcaatgc
    24601 ggcttgctat ggtgaatatg ttgctggttc tggcaaaggg ttagcaggtg ttgcatactt
    24661 cactgtggga acaggtatcg gaggtggtgc cttgttcaat gggactttcg tggaagggtt
    24721 cagtcatccg gaaatggggc atatgttgat caagcgacat ccaaaggaca atttttcagg
    24781 aaactgtccc ttccaccaag attgtttaga ggggatggca gcaggaccag cgattgaaaa
    24841 acggttgggt gtaaaaggcc agaatttatt ggctgacgat tcgttttggc agattgaagc
    24901 cttttacctt gcccaatgtg catacaatac aacactgatg ttttcgccag atagaattat
    24961 ttttggcggt ggtgtaatga aacaagaaca catgaagaaa aaagttcagg ataaatttgt
    25021 tgagcttata aatggatatg ttgaaatacc accaattgat agctacatta ttacgcccga
    25081 attaggtgat aatgccggaa taatcggcgg attagcattg gccagaaaag cggtaagaaa
    25141 taagcaacca taattaatga atgtttcatt gatactcatg tcaaaagtat ttaaccgagt
    25201 aatcaaatga tcaaatgaag ttggataaag cgaaaatgaa ttcaaataaa aaggctctaa
    25261 gtcaaatcgt gttgttttca ttaaatcact gaaaggaaat gcttctgcgt ttcctttttt
    25321 ctttttgttg tgcgctgtat accttgagga tattcactgg tggcgtacac ttaaatagta
    25381 atatattgtg ttactttatg tctgtggtaa tagttatagt tgcttaccaa tgtatgttca
    25441 tttttcgaaa tgttgacttc ataatccttg gcttaatgtt tgaatttttt tggtgataga
    25501 ttttttcagc cgtataaaat aaatcgtaat tctattatag agagttgtga ttataaaaag
    25561 atagcaagaa aaaaaaagaa agtttctata cattttaaat attttattcc tatttctatt
    25621 gctaaaaaag tagaaattct atatttacct ttaactctcg cttctcttgc aaatgtagtt
    25681 agcattttaa tcgataataa actcaggtag ttatattacc ttgaaatgat tgataatttt
    25741 gtcacttcta ttttttttgc aataaatttt ttattttagt gaaaaataag aaactatttc
    25801 tttgaaggcg ataagttaga aaaatattat tgtagtcttt tctttattag gaatcatcac
    25861 tgttgtatct tattaccttt tctgtttttc ttattagaga aaaataattt gagaggtaga
    25921 tagtttttat tggaggaagt aattatgtat atttttgaaa caaaaatttt tcattgcaat
    25981 aattgcaaca aagaaatttc atcgggggaa gaggtttgga caaaaatgga aatttccgcc
    26041 aaaagtaact gctcctcaac tgaaatcaat taaagaacta gaatttgaga atgcactggt
    26101 aatttgtatg acatgtactg atgaagtatt tcgaaaacat ttttaaaggt tgagatgtat
    26161 atagatttaa ccaatcattt atctctctcg gaaaaggaat attaactaac cagcgggtta
    26221 tcaaaaatag gtaggaaaac tgtacttaat ggcttatttc gcgctgatta tgagaatatc
    26281 agtctgtttg attaattttt agggttcaaa atacagagac aaactccagg tcgggtttcg
    26341 ccacaagcga attacccgat ctggagtttt gtctattgca ggaaggcgta gccggactgc
    26401 aggaataaag cccccggtt
//
LOCUS       DAYYDS010000033        26331 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000033 DAYYDS010000000
VERSION     DAYYDS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.033"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..573)
                     /locus_tag="ACHA02_001358"
     CDS             complement(205..573)
                     /locus_tag="ACHA02_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8669714.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            723..1205
                     /locus_tag="ACHA02_001359"
     CDS             723..1205
                     /locus_tag="ACHA02_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8669715.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1242..2252
                     /locus_tag="ACHA02_001360"
     CDS             1242..2252
                     /locus_tag="ACHA02_001360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8669716.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2367..3080)
                     /locus_tag="ACHA02_001361"
     CDS             complement(2367..3080)
                     /locus_tag="ACHA02_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669717.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3253..3860)
                     /locus_tag="ACHA02_001362"
                     /pseudo
     CDS             complement(3253..3860)
                     /locus_tag="ACHA02_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            4344..4571
                     /locus_tag="ACHA02_001363"
     CDS             4344..4571
                     /locus_tag="ACHA02_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8669718.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4587..5753
                     /gene="hemW"
                     /locus_tag="ACHA02_001364"
     CDS             4587..5753
                     /gene="hemW"
                     /locus_tag="ACHA02_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8669719.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5987..7030
                     /gene="hrcA"
                     /locus_tag="ACHA02_001365"
     CDS             5987..7030
                     /gene="hrcA"
                     /locus_tag="ACHA02_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8669720.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7124..7687
                     /gene="grpE"
                     /locus_tag="ACHA02_001366"
     CDS             7124..7687
                     /gene="grpE"
                     /locus_tag="ACHA02_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8669721.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7738..9567
                     /gene="dnaK"
                     /locus_tag="ACHA02_001367"
     CDS             7738..9567
                     /gene="dnaK"
                     /locus_tag="ACHA02_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8669722.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9718..10884
                     /gene="dnaJ"
                     /locus_tag="ACHA02_001368"
     CDS             9718..10884
                     /gene="dnaJ"
                     /locus_tag="ACHA02_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8669723.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10966..11595)
                     /locus_tag="ACHA02_001369"
     CDS             complement(10966..11595)
                     /locus_tag="ACHA02_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8669724.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            11607..11780
                     /locus_tag="ACHA02_001370"
     CDS             11607..11780
                     /locus_tag="ACHA02_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669725.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11825..12562)
                     /locus_tag="ACHA02_001371"
     CDS             complement(11825..12562)
                     /locus_tag="ACHA02_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8669726.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12564..14066)
                     /locus_tag="ACHA02_001372"
     CDS             complement(12564..14066)
                     /locus_tag="ACHA02_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8669727.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14325..16319
                     /gene="uvrB"
                     /locus_tag="ACHA02_001373"
     CDS             14325..16319
                     /gene="uvrB"
                     /locus_tag="ACHA02_001373"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8669728.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16329..19145
                     /gene="uvrA"
                     /locus_tag="ACHA02_001374"
     CDS             16329..19145
                     /gene="uvrA"
                     /locus_tag="ACHA02_001374"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8669729.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19408..20673
                     /locus_tag="ACHA02_001375"
     CDS             19408..20673
                     /locus_tag="ACHA02_001375"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8669730.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20677..21408
                     /locus_tag="ACHA02_001376"
     CDS             20677..21408
                     /locus_tag="ACHA02_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8669731.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21543..22427
                     /gene="rapZ"
                     /locus_tag="ACHA02_001377"
     CDS             21543..22427
                     /gene="rapZ"
                     /locus_tag="ACHA02_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8669732.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22424..23422
                     /gene="yvcK"
                     /locus_tag="ACHA02_001378"
     CDS             22424..23422
                     /gene="yvcK"
                     /locus_tag="ACHA02_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8669733.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23456..24391
                     /gene="whiA"
                     /locus_tag="ACHA02_001379"
     CDS             23456..24391
                     /gene="whiA"
                     /locus_tag="ACHA02_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8669734.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24934..25584
                     /locus_tag="ACHA02_001380"
     CDS             24934..25584
                     /locus_tag="ACHA02_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669735.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25771..>26331
                     /locus_tag="ACHA02_001381"
     CDS             25771..>26331
                     /locus_tag="ACHA02_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFM8669736.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8698 a         4444 c         5595 g         7594 t
ORIGIN      
        1 gaaaaaataa ttttactatg tagaaaattc aaagccattc aatcagtgat ttttttcagc
       61 acaagaaaga ctccttataa aaatagcaga tactttgtca attaagtgtt taaccaggga
      121 tgataaaaag gataggattg aacaaaacct gtataaggtg atgaatagtt tcctcgccag
      181 taaaaggttt attcgtataa agctttatcg ccaaaattta tcatgacctt ttttatcttc
      241 aatattaaat gaaatcaatt gttcaatcaa ttcccagttg attacacttt tccaaggaac
      301 tttgatgacg ttatccgtat atacgtagcc tgcttcttgg atattttttt caagggcacg
      361 aatcgctgct ttttccggtg cgatagaaaa atgattttta gcaaaactaa atgcaataat
      421 aaaggtacca tggtcagtaa acataggttg gttccatttt acagttgtgt ctagctgggg
      481 atacgtttct gttatcttag caaaaaggtc tttgatcttt tgttgatgtt ctttttcttt
      541 gatttgattg atgatcggct gaaatttttc cattctaaaa cgcctccatt aagtggtttg
      601 atttatttta ccagaaaaac aatagttatg tataaaaaac gaacaaaaca caagtaatgt
      661 tgtaaccgct atatttatat gataagatgt ctcaaataat aaaatagaaa agagacataa
      721 aaatgattga cttggcaaag aaattcttac acgaaaatat atattttgag ttgtatcatc
      781 atcgagccat ttatacaaat gaagatgctt tggtaataaa gaaagaacaa ggatttacag
      841 gtacagagac caaaagctta ttcttaaaag ataaaaaaga taataactac gtatatctga
      901 cttttactac gaaacaaacg gatttcaaag cattagggaa gatcgtcggt aaaaggcttt
      961 ctattgtttc atcagaacgc atggaagaac aaacgggaca aaagcagggc gctgtttctc
     1021 cgtttggtta tgaaaaacag attccgatta ttattgatga agagctctta tcaaatgaaa
     1081 aacttgtttt tgcaccaggt agaccagatc aaacgatggt catccaaacg gttgacttaa
     1141 acaaaatcat ccagctactt gagatgccga tatttttgat gccggcaaac gaaaaaaaca
     1201 attaaccaag taaaatagtg catacttgga ggtgaagcaa catgataagt tatccattat
     1261 tagaaagaaa tgcaacgatt ggggttactg caccatcctc aggcgtatca gaggaacacc
     1321 attcgttact cgaatcagct ttaaagagaa tggaagaaaa atctttccaa gtagttactg
     1381 gaaagacgct ttggacgcaa gataaggaaa aatcaggtcc agcaagaaaa cgagcagatg
     1441 aattcaatca aatgatgcaa gatgaaaata tccaaatgat ctttccgact tggggaggag
     1501 aattattgat tgagattctc gattttgtcg attttgatgc tctacaacca aaatggatag
     1561 taggttattc agatattagt acgttgctac ttgctgtcac attaaaaaca gggatagcca
     1621 ctgcacatgg aactaacctt attgatttaa gaggagagta cttggatgaa acgacagcaa
     1681 tgtgggaaag tgtactgaaa acaagagaag gagaatcgat tctacagcaa tcttctgaaa
     1741 agtatcaaaa agtgtggccc catgagcatt catcatcttc tgttttccat ctcactgaac
     1801 caacaaattg gcaatcagtc agcggagatg cagtaaggat gaagggacga ttattaggtg
     1861 gttgtattga tacgatcagg catttaatcg gtactcctta tggagatgtc caatctttta
     1921 aaagaaagta taatgatgga aagccgatct tgtggtttct agaaaactgt gagctatcgt
     1981 cagttgattt aagaagatca cttgttcaga tgaaactggc aggctggttt gatggcagct
     2041 cggggatcat gttcggtaga agtgcggcag aggatttgtt agattataaa atgaaggatg
     2101 tatacgaaga tctgtcaaag gaattaaaac taccgatctt gtatgatatt gattgtggtc
     2161 atgtaccgcc acaactgaca ttgattaatg gtgcctatgc agaagtagcg gtagaagata
     2221 gaaaagcgac tgttttgcaa acgtttattt aaaaaatttt ttcctgcctt ggagtctatc
     2281 aaagaccaat aaatctaaat agaaaagaaa gtgaaagaag ctgacaaaat gctgtcagct
     2341 tctttttgta taacatatta gaaagtctaa ttatctataa ctgtcacatc gattaactgt
     2401 ttcacgtcat tgggatttcc tgtaggaata gctactacag atgcattatc aatgatttct
     2461 tgagttgttt ctctaatgaa cgctggataa gatgtttgat ctgcaggcat ttgccaatgt
     2521 gaaggaactg gataactagt gattgtcccg tcatggttac ttgaatacac taaaactccc
     2581 tggtaactgt atttcccagt gagcataacc acatctttcg gatagactgc actccctgca
     2641 tcataagggg cgataggtgt tcctgcagga taatgaataa catgtaattc gaaatcaccc
     2701 gatgtatctc taaaagtatt acctctaata gctagccaaa cacgtgcgta ctcaatttgt
     2761 agatcagaat atcctgctaa cggatctatt gactgagaag actgtgagga ggttgtgtct
     2821 attgttgaag aagaaatatt tgtctcaact gtactactag atgtagaaga agtattgggc
     2881 tctgagactt tactgcttgt tgaagatgta ttcgtttcag tagaagctga tttctgagta
     2941 cttgattttt cagctgaact ggtttgtctg attgaacttt tgtctgtggt cgcagatgga
     3001 tcactatttt ctttactagg agacgtacat cctacaagga ataaagagac caacaataaa
     3061 ataaaaccta actttttcat ataaaatacc tcattatttt tttaatacca gtatagcata
     3121 atcctgaatt tttgaatccg taaaaccttg tctatgaata taaatagagt gtatcttctt
     3181 tgataaatgt cagaaaaagt gtgacaaata atcatcagta aaattacttg tcacactatt
     3241 gataaggagt atttaattga agagaatgat taggagaaag gatctccgta ctttttatag
     3301 taatcataga atccaagttt gtatagacga atcgtttttt ctcttatttc ttctgtttca
     3361 aaatggactg cttgaagaat caagtctgtg aattctatag gagctgttcc attcgcagta
     3421 atcaaattac gatctttcac agcttgttgt tctataaaat cttctttatt ctgataattt
     3481 tccgattcta accaaggaaa ttgcgcattt ccggtgtgtt tgtaaccttg caataatccg
     3541 ctctgagcta aataatcaac tgccccacaa atagcagcta ctggtacgtc tgtttttaac
     3601 aatttatcga ttatttgata taaatgatga ttttttatgt cccatgaatt gccaccgatc
     3661 ataataaaaa ggtcaacttt ttcaggtatt tgttcgatta agtaatcgat ttttgttttt
     3721 aatcctccaa tggaagtgac ctctgattgg atagaagcag ttttgttttg ccatttctca
     3781 ttttggttta gtgtgctagt aatataagca ccttcccaat cggcatattc ctctaataga
     3841 aacgtaatcg cagttttcat tttcaagctc tcctttacta tctataacaa ggacaacagt
     3901 tttaacctac agacattcat taaaattggg tagcgacagc gaaaacaaca attcttaagt
     3961 tttagacaga gtatacagat agtcaactta attaaatacg aactaaataa aaatgacagg
     4021 taatcatttt tgtttaccag cattataaca ttttgcatac atggatgaca aaagaaccaa
     4081 ttcccacttg cttgagtaca atattaaaag aaactggaag gcaaaaagta attatagaaa
     4141 cgaaaaataa atcatatcgg gcactcagac atgaaaacaa ccctagagat atataactag
     4201 gtaaccagtt taatgaaaga aaaaatcatc caagaagtcg attcttggat tttttagtgg
     4261 atttgttcgc caaatacgtt tagtatgcgt atatgagagg ttgtaagcaa agcgttgtgg
     4321 tctgagcact taaggaggaa tctgtgaaaa tagcttttca tatttttgca gattttgact
     4381 taatgccgaa ggtcagcttt gcgattaccg tttatcgaga ggctgtaaaa gcaccgtgta
     4441 gctccgatga caataagcaa gcatttttag agctaggttt ccttgattct aaatatgcgg
     4501 cttatttcaa tgagttggtg cttgaacgcg attttaagaa ggtcgaaaac aggcacgact
     4561 tttcgacgta aggagaaaga atttcaatga tagaaacaat aaatcccgca gaaaacaagg
     4621 tttctgcgta cattcatatc ccattttgcg agcatatctg ctattactgt gattttaata
     4681 aagttttttt agaagggcag ccagtagatg agtatgtgga aatgctgctg aatgaaatga
     4741 aaataatgct agaaagtcat ccgacagcga atttagaaac actttacgta gggggcggga
     4801 caccaacttc tttatctgct aaacaacttg atcgactgtt atcaggtgct agagagatcc
     4861 tgccttttca aaatggtaat gagtttactg tagaagcgaa tccaggtgat ttgacccgtg
     4921 aaaaacttca agtaatgaaa aactatggcg tcaatcgttt atccatggga gtgcagacat
     4981 ttgataatcg cttattgaaa aaaattggac gtaaacacac cgcagaagat gtgtatcaaa
     5041 ctatgcaatt tttagaagca gaaaatttta ccaatgtaag catcgatttg atttatgcat
     5101 taccgggaca aacactagaa ggatatctgg atactttgga aaaagctttg gctttggatc
     5161 tgcctcacta ttctttgtac tcattgatcc tcgaaaacaa aacaatgttc atgaactggg
     5221 tacgacaagg aagactcgaa ctgcctgatc aggaaacgga aaccagaatg tttgaagaga
     5281 cgattgaagc aatggaaaag catggacgtc accaatatga aataagtaac tttgggctct
     5341 ccggacatga atctcaacat aacctcatgt actggaacaa tgaccactat tttgggtttg
     5401 gagctggtgc tagcggttat cttggagata ttcgttaccg caacaaagga ccaatccaac
     5461 actatttacg cccattacga gcaaatgaac gcccggtatt ggaagaagaa aaattgacac
     5521 gaaaaaatca aatagaagaa gaaatgttct taggactaag aaaaaaaaca gggattacta
     5581 aaaaacattt ctatgatcgc tatcaaaaaa caattgaatc ggtgtacggt gatgtgctaa
     5641 gggaattaga gcaagaaggg ttacttgtta atgaagaaga ccgtgtttat ttgacaccaa
     5701 gagggacctt tttggggaac aatgtgtttg agcgcttttt gtttgaaaaa tagtttaaag
     5761 aatatttgtt ttttatctga atttaaataa aaaaagttca aaaatacaat aaaggactat
     5821 tgaaacgttg atataactgg atttacatca attatttaga attagcactc ttgtgtttag
     5881 agtgctaaaa aatggagtct ttctcttgac aggagaaggg cttcttgcta tatttataag
     5941 tggattagca cttagaatat atgagtgcta atgagaggtg aggaagatgt taacaaagcg
     6001 acaaaacgac attttacgtc tgatcatcca acactatacg agtagtggcg tgcccgttgg
     6061 ttccaaaaca ttgatgtctg agggagtcaa ggccagctca gcaacgattc gcaatgatat
     6121 gaaagcatta gaagatgaag gattgctgtt gaagactcac tcttcttctg ggcggattcc
     6181 atctgcttta ggctatcgtt attacgtgga tcatttgtta aaacctgctc gcgtagcaga
     6241 agatgatttg ctgctgattc gtcagtctct aggcagagaa tttcatgaaa tcaatgagat
     6301 tattaagcaa tctgctgaga tattatctga gctgacaagc tatacgactt tttcattggg
     6361 acctgaaata aaagaccgcc gactgactgg ctttcgtatc gtacccttga ataatcgaca
     6421 gattattgct atcgtcgtaa cagatatggg gaatgtagaa agccaagtgt tcactcttcc
     6481 cgaatatatg ggaagtcagg aactagaaaa aatggtacag attgtaaacg accgtctagt
     6541 cggagatcct ctgatgacgg tgtaccataa acttcgtacg gagattccga tgattcttca
     6601 taaatatttc caaacgacag aagggatgtc tcagttattt gacaccgttc ttggtcatgt
     6661 ttttgaagat aaggtttatg tcagtggaca aatgaatctc ttggattatg agccccgtca
     6721 agatcttgat cagttcaaat cgatgttttc ttttatgaaa gactcagacg gacttacaca
     6781 aatgatcact ccgatggata gcaatattca tatcaagatt ggtgctgaat taggcaatga
     6841 tttattgcag aatatgagta tgattcaagc aagttacgag atagatggtc atggaagagg
     6901 tacaatagcc ttgttagggc ctactagtat gccttattct cggatgttcg gtctagtcga
     6961 tgtttatcga agagaactag cagctaaact tgctgattac taccgttcgc ttgacttgtc
     7021 aaactcgtaa tgtgaataac gaagctattt gcagctgatc gttctctctt tcttagtaag
     7081 agcaatcaaa cagctgactg aataatgaaa ggaagactgt atcgtgaaag aaaatcaaga
     7141 agaactagat aaagaaatga cagatgctca gccagaaccg gaaatcgatg tcgaagctgc
     7201 agaagattcg ggtatttcag aagctgaagc ggaagaattc gaaacagcga aattaaaagc
     7261 tgagttagaa gaaatggaag ataaatactt gcgggctcgt gctgaaatcg caaatatggc
     7321 aaatcgagga aaaaatgagc gtgagcaatt acaaaaatac cgttcacaag atttagccaa
     7381 aaaattactt ccatcaattg acaacctgga acgtgcatta gccacagaag tgtcagatga
     7441 tcaaggggca ggtttgaaaa aaggcgtgga aatggtcttg gaaagtttgc gtaacgcact
     7501 ggaagaagaa ggaattgaaa aaatccctgc taaaggagaa gcctttgatc ctaatcttca
     7561 tcaagcagta caaactgttc cagcgactga agatacacca gccgatacga ttgtagaagt
     7621 actacaagaa ggctacaaat tacatgatcg cgtgttaaga ccaaccatgg tcattgttgc
     7681 acaataattg tgcggatata aaacttacac tttcaattaa agaaggagac gttaattatg
     7741 agtaaaatta tcggtattga cttaggtacg actaactctg cagtagcagt attggaagga
     7801 aacgaagcaa aaatcatcgc taacccagaa ggaaaccgta caacaccttc tgttgtatca
     7861 ttcaaaaatg gagaaatcca agtaggggaa gttgcaaaac gtcaagctgt gacaaaccca
     7921 aatacgattt catcaatcaa acgccatatc ggtgaagcag gttacaaagt agatgtagaa
     7981 ggtaaatcat atacaccaca agaaatctca gcaatgatcc ttcaatatat caaaggattc
     8041 gcagaagact atttaggtga aaaagtcgaa aaagcagtta tcactgtgcc agcttacttt
     8101 aatgatgcac aacgtcaagc aacaaaagat gctggtaaga tcgctggtct agaagtagaa
     8161 cgtatcgtca atgaaccaac tgcagctgct cttgcctatg gtttagataa aacagatcgc
     8221 gacgaaaaaa tcttagtatt cgaccttggt ggcggtactt ttgacgtatc tatccttgaa
     8281 ctaggagacg gtgtctttga tgtattgtca acagctggcg acaaccatct aggtggggac
     8341 gactttgata acaaaatcat cgactacatg gtagcagaat tcaaaaaaga aaacagcatt
     8401 gacttgtctc aagacaaaat ggctttacaa cgcttgaaag acgctgctga aaaagcgaaa
     8461 aaagatttat ctggcgtatc aagcacacaa atcagcttgc catttatcac agcaggagaa
     8521 tctggaccat tgcatttgga aatgacattg acacgtgcaa aatttgatga attaacagct
     8581 gacttagtag aacgtacaaa agtaccagta cgtcaagcgc taaaagatgc aggtttatca
     8641 caatcagaaa tcgacgaagt tattttagtc ggtggatcta ctcgtatccc tgcagttgta
     8701 gaagcagtac gtaaagaaac aggaaaagaa ccaaacaaat cagttaaccc tgatgaagta
     8761 gttgctatgg gtgctgctat ccaaggtggc gtgatcactg gtgatgtgaa agatgttgtt
     8821 ttacttgacg ttacaccatt gtcattaggt atcgaaacaa tgggtggagt gttcactaaa
     8881 ttgattgacc gcaatacaac gattccaaca agtaaatcac aagtattctc aactgcggca
     8941 gataatcaac cagcagtaga catccatgta ttacaaggtg aacgcccaat ggctgctgac
     9001 aacaaaactc taggaagatt ccaattgaca gatattccag cagctcctcg tggtattcca
     9061 caaatcgaag taacattcga tattgataaa aacgggattg taaatgtatc tgcgaaagat
     9121 ctaggtacac aaaaagaaca aaaaatcacg atcaaatctt cttcaggtct aacagacgaa
     9181 gaaatcgaac gtatggtaaa agatgcagaa gcaaatgcag aagcagataa agctcgtaaa
     9241 gaggaagttg acctacgtaa tgatgtagat gcattgctat tctctgtgga caaaacattg
     9301 aaagaattag aaggcaaagt ggacgaagaa gaagtgaaaa aagcagaaac agctcgcgat
     9361 gaattgaaag ccgctgttga agcaaacaac atcgaagaaa tgaaaactaa acgcgatgca
     9421 ttgaacgaaa tcgttcaaaa cttgactgtt aaactatacg aacaagcggc acaacaacaa
     9481 gctcaagaaa acccagaagc agctcaaggc ggagcagatg atgtcgttga tgctgatttt
     9541 gaagaagtcg atggcgacga taaataatca gttcactgaa tttcgagaga aggccaaagt
     9601 tgctactttg gctttttctt ttgaaaaata gtttgaagta tcggtttttg tttcaaacaa
     9661 aatatgatat gataacaagc gacgtgaaaa tgatgagaac caataggagg aaagcctatg
     9721 gcaacaaaac gtgattatta tgaagtctta gggctctcaa aaggagcatc agaagatgaa
     9781 ataaagaaag cttatcgtaa gctttcgaaa aaataccatc ctgacatcaa taaagaacct
     9841 gatgcggaag aaaaattcaa agaagtatca gaagcatatg agattttaag tgacccgcaa
     9901 aaacgcgctg catatgacca atacgggcat gcaggcacag atccaaatta cggtgctggt
     9961 ggagctggtg gcggatttgg tggattcggc ggcttctctg gtggcggctt cggtggtttt
    10021 gaagatatct tcgactcttt ctttggagga ggcggtcggt cagtcgatcc aaatgcgcct
    10081 cgtcaaggtg ctgatttgca atatacggtc gacttgtctt ttgaagaagc gatttttggt
    10141 gtagaaaaag aaatcaagta taatcgggaa gaaatctgtc atacttgtgg tgggaacggt
    10201 gcaaaaccag gcacacaacc aactacttgt cataaatgtc atggtgcagg tacgattaat
    10261 gtagaacgcc aaacaccact tggacgagtg atgagccgtc aaacttgtga tgtttgtcat
    10321 gggacaggga aagaaatcaa agaaccttgt ccaacctgtc atggctcagg acatgagaag
    10381 aaagcacatt cagttaaagt gaatgtacca gctggtgtag aagaaggaca acaaatgcgt
    10441 ttagctggtc aaggagaagc tggagaaaac ggcggaccat ttggtgattt gtatgtggtc
    10501 ttccgtgtag aagaaagtga tatctttgat cgtgaaggtt ctgaaattta ctatgaattg
    10561 cctttaagtt ttgtacaagc agctttgggg gatgaagtcc aagtaccaac tgtccatgga
    10621 gatgtgaaat tgaagatccc tgctggaaca cagacaggta cgaacttccg tttacgtgga
    10681 aaaggcgctc cgaagttacg tggtggtgca acaggcgacc aacatgtcaa agtaaaactg
    10741 atcacaccga aaaatctaaa tgaacaacaa agagaagcat tacgtgcatt cgctgaagct
    10801 ggcggcatca aagttgaaga acagcaagaa gacggtttct tcgataagtt taaagatgct
    10861 tttggcggga aaaagcggaa gtagtttttc tagtaaaaac gctaagtcat taataattaa
    10921 aaaccagacg aacaatcata tgttcgcctg gtttttttgt agttgtcatg tttgtaccaa
    10981 caaaaaagcc agctttagaa tatgataatc gtcatagttt ctaggggtat aaccagtgag
    11041 agaatggaac ttgttcaatc gatattgcaa ggtgttttta tgaataaata attcttctga
    11101 gcaatttttg atgcttccgt tatttttact gaagcatgcc aaagtcttct tgaacaaatg
    11161 tacttcttct tctgtcaggc ttttcaaaat acgttttaca tataatgttc gttgatctga
    11221 cgggattgct ggtaaaacga tcccgaggtc taaatcttca aaaaagcgga tctcgttcac
    11281 agattgatgg aaagctatcc attcgcaaac ttgtttggca tgaaggaaag attgcggtgt
    11341 ctcttgctct gtataacaaa gcgacccaat tccaaagttt agctgtaact gatacatttt
    11401 attaaacttt gtctggattt tatcaagcaa tgatagcaat gaggctctgg aaatccgatt
    11461 gactaggata atgatttgtt gtctatgtac agtgagcaaa gcctgtccgt aataagagaa
    11521 gaccctttcg agcaactcca ataaaggatt gatccgttct tgacacaatt gctgattgtc
    11581 agaaagttgt cccacaacta cataatatgg agaagaaaaa tcaagctcaa ttaagtgagg
    11641 gtggtatcga ctatgtactt gatatcatca atgaaccgat ggtactttct tatgcgatgg
    11701 aacaggtaga gcgattgctt gtttgtgcag gaaaacaagc gatgcggctg atacgattag
    11761 gtgagaaaat aagaagataa aaatacacgg tcccttcatg gcgaccgtgc atttttggtt
    11821 tttattaaat atttaatact ttattcaaga aatcttttgt ccgttcattt tgcggatttt
    11881 caaagatttg ttgaggcgtt ccatcttcta aaaagtttcc gtcgtcaatg aacatgacgc
    11941 gattggccac ttctttagcg aaccccattt cgtgcgtaac gataaccatc gtcatacctt
    12001 gttctgctaa ctttttcatg acgttcaaga catcaccgac catttcagga tcgagtgcag
    12061 aagtaggctc atcaaaaagc atgatgtcag ggttcatcgc tagagcacga gcaatagcca
    12121 cacgctgttt ttgtccacca gaaagcattt caggatacat atctttttta tcttctagac
    12181 caactgtctg cagtaatttg attgcttttt tctctgcttc ttctctagat tctttcttca
    12241 ggtccatcgg tgctaaaatg atgttttcca aaatagaaag atgagggaat aggttaaagt
    12301 gttggaatac cattccgata tgttgtctca ccaagttgat atctgtgctt ttatccatca
    12361 agtgggcgcc gtcaataata atttcaccgt cagaaggttc ttctaaacga ttcaagcaac
    12421 gcaagaatgt actttttcct gaacctgaag ggccaatgat acagacgaca tctccttcgt
    12481 tgatggatac attgatgtcg tttaatactg tgttatcgcc gtattttttg actaaatgct
    12541 caactaagat tttttctgcc atcttacttc accttctttt ctaaaacttt tgctaatttt
    12601 gtcaatgctg tgatcaaaat caagtacatg atcgcaatga tcaagtaaac atacgtactt
    12661 tgtgttgtac gtgcaacaat gatcttacca gtttgcagca attcgactac accgattgcg
    12721 gaaatgatcg ttgtgtcttt taatgagata acgaactgat tgacgaatga tgggatcatg
    12781 atgcgtatcg cttgcggcaa aatgattttt tgcattgtcc gattataaga aagacctaaa
    12841 cttctggaag cttccatttg tccgacagga acggcgttga tcccgcctcg aacaatctct
    12901 gcgatataag cacttgcgtt aagagtcaag gtgataacac cagcaaggaa gtcaggaatc
    12961 gtaaatccaa cgataccagg caacccaaag aaaataaaga acgcaagaac catcattgga
    13021 attcctcgga tgatatcgac ataaatacta gcaattgtgc gaagagcgcg tattggtgct
    13081 acactgaata atccaaaaat cacaccgatt actatagcca aagcaaagga gatcaaagtt
    13141 aggagaattg ttctccaaag tcctttcaac aatgcttgat agttgttttg gataagtcca
    13201 agaaatgttg attcatcagc agtggaagac tcactggaag tttctgaacc atccgctaca
    13261 tattctttaa taatcttatc gtattcgcct gttcttttta attctttcaa tccttcattg
    13321 aacatattaa gaagctcagg attttgtcct tttttaacgg caaatgcata ttttccgcca
    13381 gattcttttt tgatcggtgt ttccaattct tgtccttgtt tgatcgcata accgattacc
    13441 ggataatcat ccatcattgc atcgattgcg ccaactttta acccgtcgta caattgatca
    13501 gcagcatcaa aatatttgat agaatagcca tattcagatt catggtcgct taaaaattca
    13561 gcactttcgg taccaacttt tgctccgact gtttttcctt tcaaatcttc ataagacttg
    13621 atcgaagtat ctcctttttt gacagctaat tggatcccgc tatcaaagta agaatcagaa
    13681 aaatcatagc ttttctttcg ttcatctgtt acagtcattc cggcaatcat tccgtctgct
    13741 tgtcctgatt ccaaagcttg caaagcagca gagaaaccac ggtagtccat tcggatgtta
    13801 aatccttgca gtttggcgat gttttccatc aattttacat cgatgccttc gtactcgccc
    13861 tttgcgtttt gaaattcaaa tggagcaaaa gcagagtcac tggcaattac gtatacgtct
    13921 tttttgggct ctattttttg cattgtctcg cttgtgcttg actctgccgt tgtagtgttg
    13981 tcagcttgtg caacatgacc aaacgcaaat gtcacaagtc ctgtcattaa gaaaacaaat
    14041 gagtgaaata aatgctttct tttcataatc aacctcctaa atttccaata ttttattatt
    14101 ataggcgaaa agtgctgaat cagacaagac tgtaagctcg aaaaaatgaa aaaaatgcga
    14161 gtttctaaca ttttatgtta taaaacataa catgtataat aagataaaaa agtggcgaaa
    14221 gtatgttcgg tgtggtataa ttttttagta aggaatgcct ttgctttcct tttcttttta
    14281 ttggtcgtaa aatgaaaagg aatgaatgaa aaggaggacg aagaatgatt gagagagata
    14341 cctcccgaca ctttgagctt gtctcgaaat accaaccggc tggtgatcag cctgaagcga
    14401 ttaaccaatt agtggatggc gtagtcggcg ggaaaaaagc acaaatctta ttaggtgcga
    14461 ctggaacagg gaaaacatat acgatttcca atcttattga gaaagtgaat aaaccaacac
    14521 tgattattgc ccataacaaa acattggctg gtcagctgta cggggaattc aaggaatttt
    14581 tccctaacaa tgctgttgaa tattttgttt cttattatga ttattatcag cctgaagctt
    14641 atgtgccttc aagcgatacg tatatcgaaa aagattcaag tgtaaatgat gagatcgaca
    14701 aattacgaca ttcggctact agttcattat tagaaagaaa tgatgtgatc gtcattgcat
    14761 ccgtttcatg tatttttgga ttaggctcgc cttttgagta ccaaaaacaa gtagtttcga
    14821 ttcgacaggg agcagaacta gatcgcaatc agttgattcg tgacttagtc agtatccaat
    14881 tcgaaagaaa tgatatagat tttcagcgcg gtcgttttcg agtgcgcggt gatgtcgtgg
    14941 agatttttcc ggcttcaaga gatgaacgtg ccttacgtgt ggagtttttt ggagatgagg
    15001 tcgaacgtat ccgagaagtc aatgcactga caggtgaagt tttaggagaa acggaacacg
    15061 tagcaatctt cccggcgact cactttgtga cgaatgatga acatatggag catgccgtag
    15121 cgaatatcaa agcggaatta gaacagcgtt taactgtctt gcgaaatgaa aacaaacttc
    15181 tggaagctca acgtttagaa cagcgaacca attacgatat tgaaatgatg ttagaaatgg
    15241 gctacacgtc gggaattgaa aattattctc ggcatatgga cggacgtaaa gaaggtgaac
    15301 cgccttatac gctattggat ttctttcctg aagatttttt gatcgtggcg gacgaatcgc
    15361 acgtaaccat gcctcaaatc agaggaatgt ataatggtga ccgagcgaga aaacagatgc
    15421 tagtagatta cggtttccgt ttgccatctg ctttagacaa tcggccactt cggttagaag
    15481 agtttgaaaa acatgtcaat cagatcattt atgtctctgc gacgccagga ccatatgaac
    15541 atgaacaaac agataccgtt atccaacaaa tcatccgtcc gacagggctg cttgacccag
    15601 tgatcgaagt tcgtccaatc atgggacaaa tcgatgatct agtaggcgaa ataaatgaac
    15661 gagtcgaaaa agaccagcgg gtgtttgtaa cgacattgac gaaaaaaatg gcagaagacc
    15721 tcacagatta tttcaaagaa ttagggatca aagtcaaata tttgcatagt gatatcaaaa
    15781 cgctggaacg aacagaaatc attcgagatc ttcgattagg tgaatttgat gtgttagttg
    15841 gaatcaattt gcttcgtgaa ggattagacg tacctgaagt ttcccttgtt gctatcttgg
    15901 atgcggataa agaaggcttt ttacgtagtg aacgttcctt agtccaaacg attggacgag
    15961 ctgctcggaa tgaagaaggt aaagtaatca tgtacgcaga caaagtaaca gattctatgc
    16021 gtctagcgat ggatgaaacg tcaagacgtc gtacgattca gcagaagtac aatgaagaac
    16081 atgggatcgt tccaaaaacg atcattaaag aaatccgaga tttgatttct attacgaaag
    16141 aatcggaaga tgatacaaaa gaagcggtac aagtctcgta cgaagaaatg acgaaggaag
    16201 aaaaagacac gttattgatg aaattagaaa aagaaatgaa agatgcggca aaagctttgg
    16261 attttgaaac agccgcgaat gtgcgagata tgattttaga attaaaagct tcaaagtagg
    16321 aggacaacat ggctaacgat aaaattacga ttcatggtgc acgtgcccat aacttgaaaa
    16381 atatagatgt aacgattcca cgagataaat tcgtggtcgt tacaggtttg tccggttctg
    16441 gaaaaagttc cctagcattt gatacgctgt acgctgaagg gcagcggaga tacgtagaga
    16501 gcctttctgc ttatgctcgt cagtttttag gacaaatgga taagccggac gtagatagca
    16561 tcgacggatt gagtccggcg atttctattg atcagaaaac aacaagtaaa aatcctcggt
    16621 cgacagtagg tactgtgacg gaaatcaatg actatttacg cttgttgttt gcacgtgtag
    16681 gacatccgat ttgtcccaac gatcatatcg agattaccag tcaatcagta gatcaaatgg
    16741 cagatcaagt aatggctttg cctgagcgca caaagatcca agtgttggca ccgatcgtgg
    16801 taaagaaaaa aggacaacat aaaaaaatat tcgaacgaat ccaaaaagag ggatacgtcc
    16861 gcgttcgagt cgatggtgag atctatgatt taagtgaagt gcctgagctg gagaaaaaca
    16921 aaaaacatga tatcgcgatc gtcatcgacc ggatcattgt gaaagaaggg attcgttctc
    16981 gcctatttga ttctttagag gcggccctgc gattagcaga cggctatgct ctggttgacg
    17041 tgatcggtga agaagaaatg ttgtttagcg aacactatgc ctgtccttat tgcggattta
    17101 ctgttggcga actagaacca agactgttct cattcaatgc gccttttgga gcttgtccag
    17161 actgtgatgg tttaggtgtc aaattagaag tcgatcaaga tttagtgatt ccagatagaa
    17221 cgaagacact tagtcaaggt gctatcgttc catggaatcc aatcagctct cagtattatc
    17281 ctcaaatgtt agcgcaggct tgtgaaagtt tcggtatcga tttagataca ccattcgaag
    17341 aattaccgga agagcatcag gagatcatct tgaatgggac agatgatctt ttccatttcc
    17401 attatgaaaa tgattttggt ggcgttcgcg atgtagaaac gactttcgaa ggggtattga
    17461 agaatatcga gcgtcgttat catgatacga acagtgattt cacgcgtgag caaatgcgtc
    17521 tttatatgac agaattaact tgtcagacat gtaaaggtta tcgattgaat ccccaagctc
    17581 tatctgttca aatcaatgga acgaatatcg ggcaaacaaa tgagttgtct attcaaaaag
    17641 cagtagattt cttctcaaac ttaacgttat ctgaacaaga gaaagtaatt gccaaaccga
    17701 ttttaaaaga agtaaatgac agactttcat tcttggaaaa tgtgggacta gactatctaa
    17761 ctttgagtcg cgcttccgga acattatctg ggggagaagc gcagcgaatc agattggcta
    17821 cacaaattgg atcgaatctt tctggagttt tgtatatttt agatgaacca tcgatcggat
    17881 tgcatcaacg cgacaacgat cgactgattg gctcattaaa gaaaatgcgt gatcttggca
    17941 acaccctcgt tgtagtcgaa catgatgaag atacgatgtg ggcagcagat tatttgattg
    18001 acgtcggacc tggggccgga gaacaaggcg gacaaatcgt ggctgcaggc actcctgaag
    18061 aagtagctaa tgatgaaaat tctttgacag ggaaatatct ttcagggaaa aaatcaatcc
    18121 ctgtaccaaa agaacgccgt aaaggtaatg ggaaagcaat caagatcact ggtgcttcag
    18181 aaaacaatct gaaaaatatt agtgttgaat ttccattagg agaatttgta gcagtcacag
    18241 gtgtgtctgg ttctggaaag agtacattgg tcaatagtat cttaaagaaa tcgttagcgc
    18301 aaaaattaaa taagaattct gctaagccag gtaaattcaa gacaatttcc ggctacgaaa
    18361 gtatcgaaaa gatcatcgat atcgatcaaa gcccaatcgg ccggacgccg agaagtaatc
    18421 cagcgactta tacaagtgta tttgatgata tccgtgggtt atttgctcaa acgaacgagg
    18481 caaaaatgcg gggttataag aaagggcgtt ttagtttcaa cgtaaaaggc ggtcgttgtg
    18541 aagcttgtcg cggggatgga attattaaga tcgaaatgca ctttttgcct gatgtctatg
    18601 ttccttgtga agtatgtcat ggtaaacgat ataattctga aacattagaa gtgcattaca
    18661 aaggaaaaag cattgctgat attttggaaa tgacagtaga ggatgctgta gaattcttca
    18721 agcacattcc aaagattcat cgcaaactgc aaacgattgt tgatgttggc ttaggttatg
    18781 tgacgatggg gcaaccagca acgacattgt ccggtggtga ggcacaacgg atgaaacttg
    18841 ccagtgaatt gcacaaaatc tctaatggaa agaatttcta tatactagat gaaccaacga
    18901 caggacttca tagcgatgac atcgcccgct tgttgcatgt attacaaaga ttagtagatg
    18961 ctggtaacac agttttagtg attgaacaca atctagatgt aatcaaaaca gcagattata
    19021 tcattgattt aggaccagaa ggtggagaag gtggaggaac gatccttgcg actggaacac
    19081 cagaagaaat cattaacgta aaagaaagtt atacaggtca ctatttgaaa aaaataatgg
    19141 tataaagagg atagatagct gagatagtta aaaaaacata aaaaaatgaa tttttgcttg
    19201 ttttattaat atcagtgttc tagaatcatt acatggtagt tctttctaca ctgattggta
    19261 tagataaaaa cttcctataa gttcaatccg ttcaaaaaag aattgagcaa tcggaggact
    19321 ttcctttgat tgcttttttc tttatggtat gatattcctt gtgtagttgg aaaagaaaaa
    19381 caagagatta tgagtaagtg agagataatg atgaacagta ttgaaaatga agaatttatc
    19441 cctatattat taggaagcga catgaacgtt tatggtatgg cacgttcttt taatgaagca
    19501 tacggcaaaa tctgccaagc atatgcttcg gatcagttag cccctacccg ctacagtaaa
    19561 attgtcaatg tagaagttat tccaggattt gataaagacc ctgtatttat cgagacgatg
    19621 cttcgtttgg caaaagaaag atattctgat aagagtaaaa aatatctttt gattgcttgt
    19681 ggtgatggat atgcagaatt gatctcccaa cataaacaag agctgtcaga atattttatc
    19741 tgtccatata ttgattattc tttatttgaa aggctgatca ataaagtaag cttttatgaa
    19801 gtttgtgaag aatatgatct tccgtatcct aaaacattga tcgtcagaga agaaatgtta
    19861 gtaaatggac atttggaaca ggaattgcct tttgaattcc cagttgcttt gaaacctgca
    19921 aacagtgtgg agtatctttc tgtccaattt gaaggaagaa aaaaagcttt tattttagag
    19981 actagagaag agttcgattt aattttaggc cggatatacg aagccggtta taaaagtgaa
    20041 atgattgtac aagattttat cccgggtgat gatagtaata tgcgtgtgct aaatgcgtat
    20101 gtagatgaag atcatcaagt tcggatgatg tgccttggtc atccactatt agaagatcct
    20161 acaccagcat cgattggtaa ttacgttgtg atcatgccag attataacga aaaaatctat
    20221 caaaccatta aggcattttt ggaaaaaatc gaatatacag gctttgccaa tttcgatatg
    20281 aagtatgatc ctagagatgg agaatataaa ttatttgaaa tcaatttgag acaaggtcgt
    20341 agcagtttct ttgtgacatt gaacggcttg aatctggctc gttttgtcac agaagatcga
    20401 gtgttcaaca aaccctttgt tgaaacgacc tatggaacga atcaatcaga taaagcaaga
    20461 ttatggatgg gtgtgcccaa aaaaatcttt ttagaatatg ctcgggaaaa tgaagacaaa
    20521 aaactagctg agcaaatgat caaagaaaat cgttacggta ctactgtatt ttatgaaaaa
    20581 gaccgatcaa tcaagcgctg gttgttgatg aaatatatgt tccataacta tattccaaga
    20641 ttcaaaaagt attttcatgt gaaagaaggc tagttcatgg agaatttttt cagtatttta
    20701 ggcggaatgg gcacgatggc gacagaaagt tttgttcgct tgatcaacca ccgaacgaaa
    20761 gctacaaaag atcaagaata tttgaattat gtcttattca atcatgcaac agttcctgat
    20821 cgtacagctt atattttaga tcgatcagaa gaaaatccaa tgccgtttct gctggatgat
    20881 attgagaaac aaaatttatt gcgaccaaac ttcattgtgt tgacttgtaa caccgcacac
    20941 tacttttttg aagagttaca agcggcaacg gatattccta tcttgcatat gccaagagaa
    21001 gcagcaaatg aacttgtccg tcagcatacg acagggagag tagcgattct aggaacagaa
    21061 ggaagtatga aagctggaat ctatgaacgc gaagtcaaaa atcttggttt cgaaacagtg
    21121 attcctgata ctgcgcttca agaaaaaatc aattacctga tctatcatga gatcaaggaa
    21181 tcggattatt tgaatcaaga gctctattac gaaatcttag aggaagcggt agagcgccta
    21241 aactgtgaaa aagtcatcct aggttgcaca gaattatctt tgatgcatga atttgctgaa
    21301 gataatcatt acccagtgat cgatgctcaa tctattttag cggatcgaac gatcgaacgt
    21361 gcattagctg aaagaagcga ggcattggat acagcatcgg aaaagtaaaa aaattggatt
    21421 tggggcaaat atcctaaaag atatttggtc caaatctttt tgtaaaagaa aaagaaaact
    21481 ggtaatgata cgcccgggat gatataattt tattatgcac aatgtgagag gggttcgtaa
    21541 atatgacaga taatttgcaa ttagtcatca taacaggtat gagtggtgca gggaaaacag
    21601 tagctatcca aagttttgaa gatatgggct atttttgtat cgataatatg ccgcctcgat
    21661 tgattccaaa attctgggaa ttgatcaaag aatcaggtaa agtcacaaaa atggctttag
    21721 gagtggattt gcgttctcgt tccttctttg aagaaatcca aaacatgctg atcgaaatcg
    21781 aaaatacgaa cttcatcgac acttctatct tatttttaga tgcttcagac gaagaactag
    21841 tggctcgcta caaagaaaca agacgagcac atccaatggc gatggacggt ttgatcactg
    21901 aagggatacg taaagaacgg gcgatactag acgacttgaa agcccaagct tccgtgatca
    21961 ttgatacgac aacattgaca ccacgacaat tacgtgaaaa aatcaatgag tcattcaaat
    22021 caaagggaga ccaaggattt cggatcgaat ttgtatcatt tggttttaaa tacgggttgc
    22081 cgatcgattc ggatattgtg atggatgtgc gttttttgcc caaccctcat tatatcccgg
    22141 aactaagacc attgaccgga aaagatcagc cagtctatga ttatgtgatg tcctttgatg
    22201 aaacgcaatc attctaccat aaatttttgg atcttctgga aactgtattg cctggataca
    22261 ttaaagaagg aaaaagcagc ttgacagttg caatcggctg tacaggcggt caacaccgtt
    22321 ctgtggcatt gactgaacgt ttggcaaatg ctttagggaa aaactataaa gtcaatgtga
    22381 cacatcggga caaggataaa aggaaagaga cggtgaaccg gtcgtgaaaa tgaaaaccta
    22441 ccggataaga aaaccaaaga tcgtagtggt cggcggtggc actggtctgc cagttatcct
    22501 gaaaagttta cgaaatcaag gagcagatat cacagcagta gtgactgttg cagatgatgg
    22561 tggaagcagc ggtgcaatca gagaatcgat cgctatggct cctcctggtg acttgagaaa
    22621 tgtcttagtg gcgttatcgg atatgccaca attctatgaa gatattttcc aataccgttt
    22681 caaaaaggaa gatcagtttt tagccaatca tacgatcggt aatctgatca ttgccgcagt
    22741 ttccgagatg cggggaagca cttatgaggc gattcaatta ttagcaaaaa tgatgcatgt
    22801 agatggacat gtttatcctt cttcagaaac tcctttgacc ttgcatgctg tttttaaaga
    22861 cggaacgact gcagtaggag aatctaaaat cgcgatcaac cgtaaaacca ttgaccgtgt
    22921 gtatgtctgc aacacaaatg ataaaagtga agccaaagcg gctcgaaaag tcgtttcagc
    22981 aattatggac gccgacatgg tcgttttggg acccggaagt ttattcacca gtatccttcc
    23041 aaatttagtg ataccagaaa tcggcgaagc aatgctcaag acaactgctg aaaccgtcta
    23101 tatctgtaat atcatgaccc aaaaagggga aacggaacat ttcacagatg ccgatcatat
    23161 acgtgtttta cacaagcatt tgaatcagcc ttttatcgat acagtattgg tcaatacgga
    23221 aaaagtgcct gaagattata tggacccaga gatctatgat gagtatctag tacaagtcgc
    23281 acacgacttc aacggattga gaaatgaagg atgtcgagtg atttccaccg actttttaga
    23341 attgaaagat ggtggtgtct tccacgatgg agacaaagta gtagaagaat tgttccgttt
    23401 agttttcggt tcaaaatatt aagcaattta ttgttggaga aaggaggaga ataggatgtc
    23461 atttgctgca gatgtgaaga aagaactaac tggtttggaa gttcatcgag aacatgcgaa
    23521 agcggaatta gctgctttgc tgcgaatgaa cggctcttta ggtattgtga accagcagtt
    23581 tattttgaac gtccaaacag aaaatgctgc aattgccaga cgtatctatt ctcttttgaa
    23641 agatcattat caggtacgaa gtgagctact cgttcgtaaa aaaatgaaat taaaaaagaa
    23701 caatgtctat atcgtccggt tgaagcaagg cacaaaagat gtgttgatgg atcttgatat
    23761 catggatggt atgatgttca atggacacgt ttcggacgag attatgggaa attcgcaaaa
    23821 aatgcgttct tacttacgtg gtgcttttat ggcctcaggt tcagtcaata atccggaaac
    23881 gagtcgttat catttggaaa tattttctat ttatgaacag cataaccaag acatttgcga
    23941 tatgttaaat tattacggtc tacatgctcg aacgcttgaa cggcgcaatg gctatatttc
    24001 gtatttaaaa ggagcagaaa aaattgctga tttcttaaca ttgattggtg caaccaactc
    24061 aatgctccga tttgaagatg ttcgaatcgt acgggatatg agaaattcgg tcaatcgtct
    24121 agtcaattgt gagacagcaa atctgaacaa gacgattgat gcggcttcta aacaaatcga
    24181 aaacatcgaa ttgatcgaag caaaggtcgg tctacatgcc cttcctgaaa aacttcagga
    24241 aatcgcagaa cttcggcttc aacatccaga ggtcagcttg aaagaattag gggaaatgat
    24301 tccctcaggt gcaatatcaa aatcgggaat taatcaccga attcgcaaaa taaatgaatt
    24361 tgcggaaaaa ctgcgtgaag aagcagttta atccaaagaa aaaactgata catgaagcag
    24421 gagatgcgaa tgtatcagtt ttttttaaaa gctatacttg cttttcttcc gtcaaactat
    24481 aattccaact ttactggagt aacataatgt tgaaaggctt ctattgtttt ttaatacaat
    24541 cgtataaaaa gcggggagac aactgaatga agattttagt tttatacata aacttttgct
    24601 taatatagaa gtcctgatat agataggatc aagtatttat ctataactgg agattaatta
    24661 aaaactggaa ggaactgctc gatcaacctt gagaactgtt tttcttactt ctggtcaaga
    24721 ttctcgtggt tggtggataa ggatggttga taagttcatt ggattagcta gtcgaaaaca
    24781 accaggtgta attaatgtag aagttttaac aaggggatgg agtgatttat tcagtgataa
    24841 tcataagttc ataaatagta attgggatgt atggttggta tacgttatac ctgaacttga
    24901 actatcaaga tttggtttag aatttgagaa tcgatgttta taaagggagg gcaaaaaatg
    24961 tgctttttta caacttttac tatcattatg attgcagtac tttttatttt tctagatata
    25021 gcaaagcgaa atacagcatt tttattatat cgggttttac ttcgagcagg tctcataaca
    25081 tttatatcga tcgttggatt ttttctattc acagttattg tatttatttg gagaactaca
    25141 gcaccaccat tgccagagat tacatatgga gaatttccgt ttagtcttga atatgaaata
    25201 gatgaggaaa gaattttgat tgaagatacg gtgattgcag aatttatagg aagttttaga
    25261 gggaatgtga caactcaacc tagacgaagt tggagtataa aacttttaaa taatagtgga
    25321 gagttgaaag catttcatta tgaattctta ataatagaaa gtgaagatgt aacaatcact
    25381 ttaagtagtg gctctgctgg atattttatg aatgatggtg gaagcaagcc acagctcaca
    25441 gtccgtgatc gtattgcagg ttttatgcat ttttctcact atgacttaga agaagctcgt
    25501 aaaatacttg cagaatatgg gatcacatta atcaattggg aatatgcgcc accgataaga
    25561 aattttttca atgattatta ttagtcttat aaaaaacaga atgtaaaatt tatgtgcgcg
    25621 agtatttagt tttccatttt ttgtttgata gatatatagc attgttgaag catgggatag
    25681 tggtgccaat tttatcaatg aggttggtca ggcagttaac ataattggtg atgctataaa
    25741 tccatttaat tggaattgga ggaattaggt gtggaaacag tgagattatg gatattcatg
    25801 aaaaattttc gttcggcttt gagatatgtt attctggaat caaaatcagg ttattatctg
    25861 attgacacag atcactatac tttttgggga ggctaccttt ttccgatttt taattggata
    25921 atcccccata aggttatgaa aattaacttg cctgcagaag aaataaatag attaatactc
    25981 aacaaagatt tacaaaggaa aagagagaaa gaacattcta atgttgcttg gataggtcca
    26041 gcatttgcag tagtgacttc ttcaattgtc ggtcctatgc tagagagtat ttatagtgat
    26101 ttttctattg gaatacgtta tttatttgtt actttaagta tagctgttct tttgtgcata
    26161 aagggttatg ttagcaagaa gaataaagat attttaacac taatcggtgt aaacaattta
    26221 tctagtacta aaattatact gtttcctgaa tctataaaag aaggatcaag gttgttgata
    26281 atttttataa tgcttatact gttttctgtt ttatttgggg tgatgtttat a
//
LOCUS       DAYYDS010000034        26134 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000034 DAYYDS010000000
VERSION     DAYYDS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.034"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..310
                     /locus_tag="ACHA02_001382"
     CDS             <1..310
                     /locus_tag="ACHA02_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8669737.1"
                     /translation="QIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNL
                     IEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF
                     "
     gene            783..1337
                     /locus_tag="ACHA02_001383"
     CDS             783..1337
                     /locus_tag="ACHA02_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8669738.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1510..1947)
                     /locus_tag="ACHA02_001384"
     CDS             complement(1510..1947)
                     /locus_tag="ACHA02_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8669739.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1995..2942
                     /locus_tag="ACHA02_001385"
                     /pseudo
     CDS             1995..2942
                     /locus_tag="ACHA02_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3213..3648
                     /locus_tag="ACHA02_001386"
                     /pseudo
     CDS             3213..3648
                     /locus_tag="ACHA02_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3664..4497
                     /locus_tag="ACHA02_001387"
     CDS             3664..4497
                     /locus_tag="ACHA02_001387"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8669740.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4742..5767
                     /locus_tag="ACHA02_001388"
     CDS             4742..5767
                     /locus_tag="ACHA02_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8669741.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5905..6030
                     /locus_tag="ACHA02_001389"
     CDS             5905..6030
                     /locus_tag="ACHA02_001389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669742.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            6055..7170
                     /locus_tag="ACHA02_001390"
                     /pseudo
     CDS             6055..7170
                     /locus_tag="ACHA02_001390"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            7253..8149
                     /locus_tag="ACHA02_001391"
                     /pseudo
     CDS             7253..8149
                     /locus_tag="ACHA02_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            8165..8989
                     /locus_tag="ACHA02_001392"
     CDS             8165..8989
                     /locus_tag="ACHA02_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8669743.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            9021..10331
                     /locus_tag="ACHA02_001393"
     CDS             9021..10331
                     /locus_tag="ACHA02_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8669744.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10479..11738
                     /locus_tag="ACHA02_001394"
     CDS             10479..11738
                     /locus_tag="ACHA02_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8669745.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11748..12620
                     /locus_tag="ACHA02_001395"
     CDS             11748..12620
                     /locus_tag="ACHA02_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8669746.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12632..13462
                     /locus_tag="ACHA02_001396"
     CDS             12632..13462
                     /locus_tag="ACHA02_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8669747.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13502..15685
                     /locus_tag="ACHA02_001397"
     CDS             13502..15685
                     /locus_tag="ACHA02_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8669748.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15746..16966
                     /locus_tag="ACHA02_001398"
     CDS             15746..16966
                     /locus_tag="ACHA02_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8669749.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16963..18423
                     /gene="gtfA"
                     /locus_tag="ACHA02_001399"
     CDS             16963..18423
                     /gene="gtfA"
                     /locus_tag="ACHA02_001399"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8669750.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18500..19606
                     /locus_tag="ACHA02_001400"
                     /pseudo
     CDS             18500..19606
                     /locus_tag="ACHA02_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19928..20137
                     /locus_tag="ACHA02_001401"
     CDS             19928..20137
                     /locus_tag="ACHA02_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8669751.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            20130..20279
                     /locus_tag="ACHA02_001402"
     CDS             20130..20279
                     /locus_tag="ACHA02_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8669752.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20433..21098
                     /locus_tag="ACHA02_001403"
     CDS             20433..21098
                     /locus_tag="ACHA02_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8669753.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21382..22467)
                     /gene="xerS"
                     /locus_tag="ACHA02_001404"
     CDS             complement(21382..22467)
                     /gene="xerS"
                     /locus_tag="ACHA02_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8669754.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22801..23034
                     /locus_tag="ACHA02_001405"
     CDS             22801..23034
                     /locus_tag="ACHA02_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8669755.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            23031..23438
                     /locus_tag="ACHA02_001406"
     CDS             23031..23438
                     /locus_tag="ACHA02_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8669756.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24575..25093)
                     /locus_tag="ACHA02_001407"
     CDS             complement(24575..25093)
                     /locus_tag="ACHA02_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8669757.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25460..25708
                     /locus_tag="ACHA02_001408"
     CDS             25460..25708
                     /locus_tag="ACHA02_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669758.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         8921 a         3984 c         4844 g         8385 t
ORIGIN      
        1 tcaaataagg tttatgatag ataaatggaa aaagcagtat ccacgagtgg ttaaattgct
       61 catgaatcct gctatattaa ccttttataa tttccctccg tcaatcagaa gaactattta
      121 ttcaactaat ttgattgaag gttttaacaa acaattaaag aaatatacca aaagaaaaga
      181 gcaatttcct aatgaagaat ctctggagag atttctagta tctcaattca acaactataa
      241 tcaaaagttt ctatgccgag ttcataaagg ttttaaagaa atacatgata cattagaatc
      301 aatgttttaa cttgatcaac agaatgggct tttccattta cacaaaatta ttgacgctcc
      361 cgacaaaatt atgatcttgc gtaaagttat aaaatattaa taaaaatatt ttattaatga
      421 aaagcttggt agcacggtta ttcatatgct aatttatttc actttatagt aaggggagtt
      481 cttagtatat tgttcaaact taaatttcat ataatttaat tatgtaaatt agctattcta
      541 agaacttcta cggataactg cccgtctaaa atttaaataa gagcttagaa tcttcatata
      601 agtaaggtta atttttttgt acaaaaaagt cggaatattc tgagttactg aaatgatata
      661 aaatcataaa ctaaatccct tgattttatt gactttcaaa agtttatata tataagttaa
      721 tgattatggt aaaatatata cataatcatt aactcagaat atttttaaga agggaaacaa
      781 tagtgagcta taatgtacaa ccgttaagga caaatgaaga gatacaagat tttcagtttt
      841 ggctaagaag aacaagtaat ccggaaagag atagtttttt atttttgttt ggaatcaata
      901 atgggttacg tatgtcagat attataggat taaaagttgg agatatacga ggcaaaagta
      961 agcctattat tgttgaacat aaaacaggta agcgaaaacc gatttttata gataatttga
     1021 gagaagaaat tttattatat actgaaggta aagaagaaaa tgattggtta tttccgagtc
     1081 ggcaacaagg tcgacatata acaagggatc gtgtctatca gatatatgca gatatagcag
     1141 aaaaattagg acgagatgat ataggaacac atacattaag aaaaactttt gggtatcact
     1201 actacaagaa aactagagac attgctacct taatgtttat ttttaatcat agctcgcaag
     1261 caattacaaa aaggtacatt ggtataactg aagatgaaat aggagcatca cttcgtggat
     1321 ttaaactagg tgtataattt aaaaattttt ataaacagta aagacaaaga ttaaaagcta
     1381 tttttatcta gaacgatttt atttcataaa gttcgcggaa taaaagctgg ggttactggt
     1441 acttggtata ggttcgtgtt atataaacta agtaattaaa taacattttt aggttggaaa
     1501 aggatgaaat taaaaaaaga gaatcaatac ggtctcaaaa acgcaaacgc tgcggaataa
     1561 gaaaaatact gataactcaa cgtttagcag actatctaat agggatagtc tgcttttttc
     1621 gtttttgacc atacttttga ccttctgcaa acttccttgc ttttcctctg tagaactcat
     1681 gaaatctgca aaacgaattg ccgtttcctc tgtctgcttt ttcgcgatat gggtgtatat
     1741 atcatccata gtggttttaa tatctgtatg tccaagtcga acatacactt ctttgacaga
     1801 cgcaccagat tcaaagagca gactacaatg ggtatgacga aaaccatgaa gtgtgatatg
     1861 ttttaagtta ttctctttaa tgatgatttt caaattgtga tttacatagt ctaaataaag
     1921 gggcttattt tcttctgatg aaaacaaaag ttgttcacca tcttttatag gtttgattct
     1981 catttttaga tattatgccc gcacgtattc atcacatttc tattgtgaat cgctttattc
     2041 gaccgacatt tgacttttat cataatatac tcggtttaaa attactaatg aaaacaatta
     2101 atcaagacga tcataccatg tatcacctgt ttttttcaga taatcaccac cgtgtgggaa
     2161 ctgaactgac tttttttgaa gtacaagaag ggaacaacca gttttttgga accaatacga
     2221 ttgaacgaac gatactaaaa gttccttccg aagcatcatt gcatttttgg gaaatatatt
     2281 ttgaaacaca aggcgtttgc cattatggaa tcgaatcatt ttctggacga cctatcttac
     2341 gttttgaagg gccagatgca acacgattag ctttagttcc cttacgcgaa tttgaagata
     2401 ttgatgatta tttcccgtat gtcacagacc agatccctac agagcatgcc attttaggaa
     2461 ttgatgccat tcaactacgc gtacaatatt ccgatgcgac tgaacgagag ttattgtcgg
     2521 taggctggta gcacaaagaa tacgtcagtt tcttttcggg tcatcaacga gtgacaatct
     2581 tagaaaatca agacactcag ttctatcaag aagttcagat tattcaagat cgtgagaatc
     2641 cagttgccat tgaagggatt ggtagcgtcc atcatgttgc gtttggcgta gaaaataaat
     2701 ctgatttaca acgcatcgac aaacaattac aagagcgcaa ttttataaat tcaggaataa
     2761 aagatcgtga attttttgta tcactttatt atcgtgaccc aaatcaatta ttgattgaaa
     2821 ttgctacagg ggaaggaaac ctagatgcaa aagcgtatga aaatcaaagt ccacgctttg
     2881 aagaaattcc tttattttta ccgcagtact taaatttcat tcgtgaacaa gtggagcaat
     2941 aattagctaa tcaaatccat gacgaagagt aagaggttga ctcataagtc aaaaaataaa
     3001 gaaaaacgca tcaaatccat tctaaagaat gttgatttga tgcggttcct atttttataa
     3061 actatcattt tcgactaata aaactactta tgagtcagcc tctctttttt tcttgatttt
     3121 tatacctctt tctaggtaaa ctaaacatct gccaaaaata ccgtgtctca ttgaaacatt
     3181 ctcaactatt tcaaaataaa ggggagtacc aaatgaacga gtatattgaa attatccacg
     3241 caagagaaac aacttaaaaa tgtcagtctc aaaattccca aaaataaaat tagtattttt
     3301 accagcgtat ctggttcagg aaaatcatcg attgtttttg atactattac ccaagaagct
     3361 ggtcgtcaac tgaatgacac ctatgatagt tttacccgtt tatttttacc taaatacagt
     3421 cgtccagagg ttgatgagat tgataatctt tctacggcga ttgtggtcaa tcaaaagaag
     3481 attggtggca gtgcgcgctc aacgttaggg acgattacta acatcaatgc gttatttcgt
     3541 gtgttgtttt catgttttgc aacacctagt ttaggatatg caaatgctta ttcatttaat
     3601 gatcctagtg gaatgtgttc tgtctgtcag ggaatcggta agacgattgc atttttaata
     3661 ggattgcttt atttttcggc tattgataat gctgattttg tattttctga tccatttacc
     3721 gcagaagttt tgtctggaat tgaggaacag gcaaagaatc atggttattt tgtattggtg
     3781 cataatgtta attcagcaga agatgttaaa actattcaga gaaattggcg ttttgagggg
     3841 ttcattgttg ttggtgtacg tgtaaaagaa tttttggaat taaataacgt ttttgatggt
     3901 cctgttgttt atattgatac gcatctttca aaagagacat taagagaact tgaacaacaa
     3961 atgaatcgtt tatttgtaaa tacaaatgat taccaagcta gtacattggc agtggaacat
     4021 ttgattcatt atggtcatga gaaaatagcg tttctttctt ttgactacat tgtaggtgaa
     4081 ccaagtgtta ttcaagaacg acatcaagct tatatcgata cattaaagaa atatcattta
     4141 acctttaaat cgcattggat atataacaac tctgaatttg agcgaattta tcaagaatta
     4201 gctgaattta ctgcaattgt tgttactgga gattatttag cggctaaatt agtaaaattt
     4261 ttaaaaaata agaataaacg agtgatggaa caattatcga ttattggatt tgatgatatt
     4321 acttttgcag aattaatgga tcctgcgttg acaacaatac gactaaatcc aggtaacaag
     4381 ggggcgattg cttttcacca actgattgga ctggccaaac aagaagaaat aaaaaatcaa
     4441 gtactactat tagagggaga attgatttct cgagactctg ttcacaaaat caaataaaaa
     4501 agctctgtga aataagtata gttaacaata aaatatccga acgatagaga gaatgaactg
     4561 tgatctagca gttcctcgtc gcaaattcac tagaattagc attatttact ctaaattgtt
     4621 cggatatttt ttcttgtttt taattatgat tacagtgcct atttttaacg aaatttgctt
     4681 attttcaaaa tgtatgatta tatgttagta aacactttca aatatgaaaa gaggagctat
     4741 tatggtagga attagggatg ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg
     4801 cgtgctaaat gaggatccga cactttcagt aaccgatgaa accaaacgaa agattgcaga
     4861 atcggttaat ttttatcatt atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat
     4921 tgcattaata acgacagttt ctgaagtaga tgagttggaa gatccctatt ttcgaacgat
     4981 tcgcagaggg attcaagtcg aagcagaaca aagcaaagtc aatttgaaga aaattattcg
     5041 tttgtcagaa acaccgttgg agcaagacga tttgctaaaa tgtcaaggtg tgttgattat
     5101 tgggcaggtg ctatcttctg tgattgaaga agtatcagca attaattcga atgtcgtagt
     5161 gattgatgac cctagtattg aagctgaagt agatgcagtt tatactgatt taacaaaagc
     5221 aacgaatcag catttagatc gattgtataa aaaaggacac cgaaacattg cgtttatcgg
     5281 aggaggacgt gttcgtttag atagccaagg aaataaacac gtgtcagatg atgattatcg
     5341 aaaaatagcc tatgagacgt ggatgaaaca aaaaggatta gaaaaatata gtcaaattta
     5401 tttagaaggc tggaccacac ttgacggaat gcaggcagca gatttacttt tgaaaaaaca
     5461 tgctaaagat aagttgccga ctgctatcat ggtaggaaat gatcctattg ctgtgggggt
     5521 ttatcgttca cttcaaaaaa atggtctaca tattccagaa gatatttcga ttgtgagttt
     5581 tgataatatt gaagttgctg aatttttaac acctgcatta aatacggtga atgtgaatac
     5641 agaagaaatt ggaaaactag cagttcgcat ggttaatgaa cgaataaaag aagttagaaa
     5701 tgtttcgatc aaagtgattg tttctaataa tattattgtt agagaaagtg aaaaaaatat
     5761 cgattaaaaa agtaaacatc caaaaaaggg tgtttacttt tttgtttact aatattttta
     5821 gtaaaaaatc tcgacaaaac attaagtaaa cagttatggt gttgttaaca aaaagaaaac
     5881 gcattcaaaa caggaggaat taagatgaaa catttcaaaa aaatagcttt aacagggata
     5941 gctgttttaa ctgggacatt actcgcagca tgtggtgggg gagatagtaa ttctaaagac
     6001 gggcaagtaa ctttaagcta tgcgagttga gatactgacc aagctccagg actaagaaaa
     6061 atgttagatg aatttgaaaa acaaaatcct gatatcaaag tagaaatgga gacaacgcct
     6121 tgggatcaat attgggttaa attagaagct gcaacaacgg gaggtaatat gccagatgta
     6181 gtaaccatgc actctagcga atcctataaa tatatgtcac agaatgcttt gatgggactt
     6241 gatgatgtta tcaaagaaaa tgatattgac atgggaaact ttactgaagg aattgctgat
     6301 ttctatacgc ttgaagacga actatacgca atccctaaag atgcttctgt agtggggcta
     6361 tggtacaaca aagaattatt tgataatgca ggtgtcgatt atccagatga aacatggaca
     6421 tgggaaacac tcagacaagc tgcaattgat ttaacagatg aatcaaaagg catctatggt
     6481 tttgcatcac atattggaac agagactggc tactggccat ttatttatca aaatggggga
     6541 gaagtatttg ccgatgacaa tacaaaatca ggtttgaata ccccagaagc acaagaagct
     6601 ttagcttttt atcgagatat tgcacttgtt gatcatgctt caccaacaat tacagaatta
     6661 caagatcctg ataagttaag tcgttttcaa tcaggtagat tagcaatgat gattgaagga
     6721 aactggtttg caccgggatt tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc
     6781 gtgttaccta agggaaaagt taatgcaaca attacaaatg gacttggttg ggctgccagt
     6841 gcgagtacaa aatacccaga agaagtgaaa aaactaatgg catttttagc tagtgaggac
     6901 gccaataaaa tccaagcgga aacgggggca tcaatcccag cactaaaagg ctttgaagac
     6961 gagtgggctg cttcttcaga tacaattgat ttgagtgctt ttggaaaaat gttggagtat
     7021 ggagtccctc gtccatacaa taaagatgga ttacgagctg aacaagcaga agttcaaatt
     7081 attaaccgta ttctgtctgg tgaagtaact gttgaagatg gaaccgctca aattgaaaaa
     7141 gaagtcaatg cgatattgag cggaaattaa attagttaat tatacaatat acaggcgaaa
     7201 ggattctatt tcgtctgtat attaatggat gaataccaaa aagggggata ttatgaaaaa
     7261 tcgaattttt ggaaaatatc gtaaagatca agtgatatgg ggctatattt ttatttttcc
     7321 aacggcatta ggattaattt tattaaatat atggccagca attcaaacag caattttaag
     7381 ttttcaaaaa acggtagggt tcggtgatac tgaatgggtc ggattaagaa attaccaaaa
     7441 aatgtttgca gacggtgaag tttttcagtc tttattaaac acattaattt atgcagtggt
     7501 ttcgatacct agtattgttg tattatcctt gttggtggca gttttaatga atagaaaagt
     7561 taaaggattg tcgatttatc ggacaattta ttttctacca gtcgttgctg caccatccgc
     7621 agtcgcaatg atttggcgct ggatgttcaa taatgattat ggactgatta ataacatgtt
     7681 ggctaaaata ggtctcgatg gaccagcatg gttaacggat ccatcgattg cgatttattc
     7741 tattatcatt gtaggagttt ggagtgctat tggttataac atggtgttat tactagccgg
     7801 cttataagaa attcctaaag attattatga agcagcggaa attgatggtg ctagtccaat
     7861 ccagcagttt ttcaacatta cgttaccatt agttacacca acattatttt ttgtagttgt
     7921 aacaacagtt atcaatgctt tccaagtatt tgatgttatt tttatgatga ttactcctaa
     7981 tagtaccgcc atgtttaaat cacaatcatt agcctattta ttctataaac attcatttat
     8041 tttaaatgac aagggatatg gttcggcaat cgttatgttc cttttagtga ttatattaat
     8101 tattactgca attcaaatga agttgcagaa aaagtgggta aactattaag gaggctatca
     8161 aattatgaaa aataaatttt tttcaaatgc gccaatccat atcatgctta ttttaggtgg
     8221 tattgcaatg gcattacctt ttatttggat gattttaact tcagggaaaa cgttggttga
     8281 atctacccaa attccaccta caattttccc tgagaaattt cagctaagta actatcaaca
     8341 agtacttgat ttattaccat tcatggcttt ttatatcaat acatttttaa tggttgttgg
     8401 acgagtgatt ggctcggttt tattttcagc aatggctgca tatgcttgtg cgcgtctaag
     8461 ttttccaggg aaaaatatcc ttttcagctt agtacttatt caaatgatga ttcccggttc
     8521 catttttatt actccacagt atttattagc acaaaaattg ggtattttaa atacaattcc
     8581 agcattaata ttaccagggg tagttagtgc gtttggtacg tttttattaa gacaattttt
     8641 cttaggttta cctgatgaat tggaggaagc ggcctattta gaaggtgcca atatttggac
     8701 gatctttttc aaaattatgt tgccgcttgc taaatcagga ttggttgcac ttggaatatt
     8761 cacagcgttg tttgcattta aagatttaat gtggccattg attgtcaata cttcaattga
     8821 taagatgcct ttagctccgg gattagcaaa cttacaagga caatatgcca cgaacttccc
     8881 acaattaatg gcaggttcgt tattagcaat ttggccaata gttgtgatat tcatcgtttt
     8941 ccaaagaaaa ttcattgaag gaattgctac aacaggtagt aaattataaa actattaatt
     9001 agtgattgga gagtaagtac atgacaaaaa ttacatttat tggtgcagga agtactattt
     9061 ttgcaaaaaa cgtattaggc gatgcgatgt taactccaag tctacaagat gcacatattg
     9121 cattgtacga tattgatgaa aaacgactaa aagaatcaaa tttaatgctt aaaaatatta
     9181 ataaaaattc gaatgaaaat cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt
     9241 taagagaggc tgattttgtt atcaatgcta ttcaagtggg tggttataaa ccttcaacag
     9301 tgattgattt tgatattcct aaaaaatatg ggttgcgaca aactattggt gacaccatgg
     9361 ggattggtgg gatttttaga accctacgga cactaccagt aatgttagac tttgctaaag
     9421 aaatggaaga agtttgtcca aatgcttggt tattgaacta tacaaatccg atggcgatgt
     9481 taaccatggg tgttttaaaa gcgacgagta ttaagactgt tggtttatgt catagcgttc
     9541 aagtatgcgt accagaattg tttgagcatt taggaatagc cgatcaatat gacttaaatg
     9601 actttcaatg gaaaattgca ggcattaatc atatggcatt cttgttggaa attacaaaaa
     9661 atggggaaga tttctatcca acaattcgtc agttagctgc agaaaaacca aatccacatc
     9721 cagattctgt acgttttgaa ttgttgaaac gttttggtta ctatattaca gaatcaagtg
     9781 aacacaatgc agaataccat ccatacttta ttaaatcaaa atacccagaa ttgattgacg
     9841 aattagggat tccgttggat gaatacttac gtcgttgtga acaacaaatt aatgattggg
     9901 aaagcatgcg agaggacatt gtaaataatg gggagttaac acatacgaga agccgtgaat
     9961 atgcttcata tatcatggat tctattacga caggaactcc gtcagtgatt gcaggaaatg
    10021 tcttaaataa aggcttaatc acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt
    10081 tagtcgataa aaatggcgtg caaccaacct atgtcggtga attgccaaca caattagctg
    10141 ctttaaatcg gacgaatatc aatgttcaag aactgacagt agaagccgct ttaacactag
    10201 ataaatcaaa aatttatcaa gcagcgtatt tagatccaca tctatcatct gaattgtctt
    10261 taacagatat caaaaattta gtggatgatt taatcgaagc acatggggac tatttacctg
    10321 aatataatta aaaagaagct gaaagagtaa ggaattttca attccttact cttttcttag
    10381 ttaaatttag taaaaatatt gacaaaacac ttagtaaaca gttacattag tgttaacaaa
    10441 aagaaaacgc attcaaattg tataaggagg agcataaaat gaagtggtct aaaaaaatta
    10501 ttttagcttg tatagcacta ggagcaacga tctctttgac tgcttgtgga aaaaatcagg
    10561 aagatgcaag cggtaaggta gaaatagagt tttttaatca aaaaaaagaa atgactcaaa
    10621 cgattcagga gattgctaaa gattttgaag caaaaaaccc tgatattcat gtaaaagttg
    10681 tggatgtacc gaatgctggt gaagtaatta aaacacgtat gcttgcaggc gatgtaccag
    10741 atgtcattaa tctttatcct caaagtatag aattgaaaga atgggcgaaa gctggttatt
    10801 tagaggactt aaccgaagaa ccgtatttag agaatatcaa aaatggatat gcacaacgtt
    10861 ttgctattga ggatcgtgta tatagcatac cattaaccgc taatgtctat ggtttttact
    10921 ataacaaaac tgcttttgag gaaatgggga ttcaagcacc agaaacttgg gcagaatttg
    10981 aaaaaatagt agctgagatt aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag
    11041 aaggctggac tttaaatggt tatcaccaac tagctatagc gactgctgct ggtggtgaag
    11101 aggaagcgaa caatgtttgg cgtttctcag aagttaatgg aattaatgct gaaagcaaag
    11161 aaatgcaaaa agattttaat cgattagatt tgttaagaga accaaaagct ttgcaaaaca
    11221 actggcaagg tgccggttat aacgatacag tagttacttt tacaaaaggt gatgcgttga
    11281 ttatgccgaa tggttcatgg gcaatgccga tgattcattc acaaaatcca gattttgaag
    11341 taggaacttt tccttttcca gcagatgaag caggtcaaag tctaacgatt ggcgcaggag
    11401 acctagcctt atcagtttct gcaacttctc aacataaaga agaagccaaa cgttttgtag
    11461 aatatatgac aacacctgaa gcgatgcaga aatattatga tgtagatggt gcaccgtgtg
    11521 ctgttgaggg cgttatcgag aatactcagg attcaccatt aagtggttta actgaacttg
    11581 cttttactga tcgtcattta gtttggttag ccaaagattg gaatagtgaa aatgattttt
    11641 ataccttaac aacaaattat ttacacaatg gcaatcaaaa agccatgatt aacgcattaa
    11701 atgctttctt taatccaatg aaagctgacg gaaggtagga aaataaaatg aaaataaaag
    11761 aaattttaaa tcgttactgg gcaatcattt ttgtagcaat tccaattatt ttgcagatga
    11821 tttttttcta ctttccaatg gttcaaggtg ctttttacag tttaactaat tggactggtc
    11881 tgacttataa ttttgatttt gtgggtttga acaattataa gattcttttt tctgatccga
    11941 agtttatcaa atcgattggc ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa
    12001 ttggttttgg tattttgatt gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct
    12061 ttagagcatg gttctttttt ccagcggtct tatcgggcgt ggttgtttct ttagtattta
    12121 aacaaatttt taactatggt ttaccaaaaa ttggtagtat gttaggcatt gaatttttac
    12181 aaaccagtct tttaggaaca acatggggag cgatttttgc agtaattttt gttttacttt
    12241 ggcaaggaat tgccatgcca gtcatcattt tcttagcggg ttttcaaagt attccacaag
    12301 aaattattga agctgctaga atggatgggg cgaatgataa gcaaattttt tggaatattg
    12361 aaatcaccta cttattacca agtatttcaa tggtatttat tatggcatta aaaggtggct
    12421 taacagcatt tgatcaaatt tttgctttaa ctggtggtgg accaagcgat gccacaacat
    12481 ctttggggtt acttgtttat aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg
    12541 ctattgcttt agtcttattt atcttaattg ggattgtttc actggcacaa ttaaaattat
    12601 cgaaaaaatt tgaaatctag gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc
    12661 taaatacttg cttttattat tagggggctt catgatttta gttcccttag tttcaacgct
    12721 attcggttca tttaaaacaa cacccgatat tatgaataat ttctttcgtt ttcctaatcc
    12781 agcgactttg gaaaatttcc gaagattgtt agcggatgga attggtcatt acttttggaa
    12841 ttcggcaatc atcacaatca ttgcagtagt tgctgtgaca gcaattattc ctatggctgc
    12901 ttattcacta gcaagaaaaa tgactcgtgg gaaagcatac ggattgatgt atttattttt
    12961 aattttagga atttttgttc ctttccaagt aattatgatt ccgattaccg ttatgatgag
    13021 taaaattggt ttagcaaata tgtggggatt aatcttactc tatctcactt atgccgtacc
    13081 acagacacta tttttatatg ttggttttat taaactaaat gtgcctatca gtctggatga
    13141 ggctgctgaa attgatgggg ctagtcactt tacaacttat ttcaaaattg ttttcccaat
    13201 gttaaaacca atgcatgcga cgactattat tcttaatgct ctatggtttt ggaatgattt
    13261 catgctacca ttattaattt taaatcgtga ttctaatatg tggacactac cattgtttca
    13321 atataattat acgggacaat attttaacga ctatggtcca agttttgctt cctatacagt
    13381 aggaattgtc acgattacac tagtttatct tgctttccaa agaagtatta tagctggaat
    13441 gagcaatgga gcagtgaaat aaccaataaa ttattctcat tataaaggag ttcataaact
    13501 tatgattata tttgacaatc acacacaaac atttcattta caaaataaaa aaattagcta
    13561 tctattagga atcgaagaaa ataattattt aacgcatctt tattttggaa aaaagattac
    13621 gcactattct ggtgggtatc gctacccccg aacagatcgt tccttctcac ccaacaatgc
    13681 aaatgcgcaa gatcgtttat tatctttaga cacattacca ttagaatttc caggatatgg
    13741 acatggtgat tttagagaac cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa
    13801 tgattttcgt tatgatagct acgaaattca aaaaggaaag cccagcttag cgggattgcc
    13861 agccttatat acggaagatg atgatgaagc tgaaacgctg attattcaat tgaaagacgc
    13921 gattagtgga ttgactttac agttgttcta cacaatttat caaaatcaca gtgtaattat
    13981 tcgttctacg caattagtaa ataatagtga tgaaacagtg gagattaacc gcttagctag
    14041 tcaatcaatt gattttccaa atcgtccact cgagttaatt catttaaatg gtacctgggc
    14101 aagagaacgt caaatgacta gagaaaaaat tcatacaggg ataaaagtat tagatagtaa
    14161 acgcggatca agcagccatc aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga
    14221 tgaatttcaa ggagaggcat atggatttaa tttagtttat agcggtaatc acgaaacggt
    14281 gattcaaaaa gatccatttg accaaacacg ggtcattatg ggaattaatt catttaattt
    14341 tggttggcaa ctaaaaactg gcgactcatt tcaaacgcca gaagtcgtga tggtttatag
    14401 taatgaaggt ctgaatacgt tatcacagac gtatcatgaa ttgtataatc accgtttaat
    14461 tcgtggaaac tatcgtttaa aagaacgtcc cacgttaatc aacaactggg aagcaacgta
    14521 ttttgatttt aacgaagaaa aaatcatgaa tattgtcgat gatgcgaaga acttgggcat
    14581 tgaaatgttt gttttagatg atggttggtt tggcggtcgt gaaaatgact tcacttcatt
    14641 aggtgattgg gttgaaacag aaggcaaact tgcttgtggt ttggaaaatt tagccaagca
    14701 agtgcatgat aacgggatga aatttgggat ttggtttgaa ccggaaatga tttctgaaga
    14761 tagtgaacta tttcgagcac atccagattg ggcattgggt attccaggtc gtgaccgctc
    14821 aattagtcga aatcaatatg ttcttgattt ttcaagacaa gatgttcgtg ataatatcta
    14881 tcaacaaatg acggcgattt ttgatcgtgt accgattgat tatgtgaagt gggatatgaa
    14941 tcgaaatatg acagaagtct attctgtctt gttggactca acgatgcaag gtgaagtggc
    15001 tcatcgttat atgttagggt tatatgaatt tttagaaaag ttaacaacga attatccaac
    15061 tattttattt gaaagttgtt cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta
    15121 catgccacaa acttggacga gcgacaatac cgatgccgtg gctcgtctga aaattcaata
    15181 tggaaccagc ttagtgtatc caatttcaag tatgggtgcg catgtttcag cagtcccaaa
    15241 tcatcaaaca ggaagagaaa cgagtttaac tattcgtggc aatgctgcga tggctggtgt
    15301 ttttggttat gagctagatc taacgcaatt aacttctgaa gaaaaagcag aaattaaaga
    15361 acaagttgcc ttttataaaa agcatcgcca acttttgcaa tatggaactt tttatcgctt
    15421 ggaaagtccg tttgaaggca atgatactgc atggatgttt gtctcaaaag accaaacaga
    15481 agccatggtc ttttatttcc gagtattagc agaagctgcg catccattag tcacattgaa
    15541 attagcagga ttagcagaag aagcaactta tcaaatggaa gatgtaacat ttgctggcga
    15601 tgaattgatg aatctcggtt tttatattga ctcagaacta catggtgatt atgcgacaca
    15661 acgcttttat ttgaaacaga tatagtagct tgaatttaca atataagtgc aacaccaaaa
    15721 gagactcatt ccaaacgaag aaaatatgtt ttatttacac gcatttacta aaaagcagcc
    15781 tgatttgaat tgggaaaatg aagaagtacg tgaagaaatc tatgcaatga ttaactattg
    15841 gttagataaa ggactaggcg gatttagaat cgatgcgata ttaaacatca aaaaaaaact
    15901 agagtatggc atctttgaat ctgatggaga agatgggtta gccttcattg ggccctggat
    15961 tcttaatcag tcgggcattg aagtgtggtt aaaagaaatg agagagcgga catttaaacc
    16021 acataatagt atgacggtag cagaagccga tgttcctaat gaacgtttag atgaatatat
    16081 tggtgaagat ggctattatt cgatggtttt tgattttagt tatacggata ttgatgtacc
    16141 tgaaactggt gaatggttta aagatagcca atggacatgg acggatatgc gtaccaatat
    16201 ttttacgaat caattagtta cgcaagataa aggttggggc gccttatatt tagaaaacca
    16261 cgatcaacca cgttcaatta ataaatatat tccagaagaa tggatcaatg attatagtaa
    16321 aaaaatgttg gcgacgcttt ttatgttact aagaggtacc ccttttattt atcaaggaca
    16381 agaactagga atgaccaaca tagaaatggc atcattagaa gattttgatg atgtagctac
    16441 gcattctcag tataaacggg cattagaata tggattggca ccagagcaag cattgaaagc
    16501 tgtcaataaa cgaagtcggg ataattctcg cacacctatg caatggaata cgcaaaaaaa
    16561 tgccagattc tcaacttctg ataatgtatg gttaaaagta aatcctaatt atccagaact
    16621 caatgcagaa gctcagttaa ccaatcaaca gtcggtattc aattattatc atcaattaat
    16681 taaattaaga aaaaatagtg tatacaaaga cgtgctaatc tacggacaat ttctaccagt
    16741 gaaagataaa aatgaacata ttttgcttta tgaacgccaa ttagaagata aaatcgtatt
    16801 agttatctta aacgttacag caacagaaca agaatattat gtagatgaac agtaccaaca
    16861 agtattaatc aataattatg aagatgtttt attagatcaa agtcgaaaat tgcgtttacg
    16921 accgtttgaa agtattgtat tagctaatta tggaggacaa ttatgaaaat taaaaacgaa
    16981 gcaatgctca tcacctatgc agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc
    17041 ttagacaaac atttacaagg tgttattggt ggtgtacatc tgttgccttt ttttccatcg
    17101 acaggtgatc gaggctttgc gccgagtgat tatacgacag ttgatccagc tcttggaaca
    17161 tgggaagatg tggaagcatt aggtgaagat tattatttga tgtttgattt tatgattaat
    17221 cacatttctc gtgaatcaaa atttttccaa gatttcaaaa aaaaccatga gcagtcacca
    17281 tacaaagaaa tgtttatccg tattcatgaa tttttcccag aaaatcgacc aacacaagaa
    17341 gatattgatt taatctacaa gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg
    17401 gatggtacga cagaagaggt ttggaatacc tttggggaag aacaaattga tttagatgtg
    17461 accaaagaag ttgtgaaaga atttattcga gaaaccatca aagatatggc gaaccatggc
    17521 tgttctttaa ttcgattaga tgcgtttgct tatgcgatta aaaaattaga taccaatgat
    17581 ttcttcgtgg aaccagatat ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa
    17641 tatgacgtcg aattattacc agaaatccat gagcattatt ccattcaaat gaaaattgcc
    17701 aatcatgatt attatatcta tgacttcgcc ttgccaatgg tcactctgta ttcactttat
    17761 agtggaaaat cagaacgttt agccaattgg ttgaaaatga gtccgatgaa gcagtttaca
    17821 acattggata cgcatgatgg aattggtgtt gtggacgctc gtgatttatt aacagatgaa
    17881 gaattggatt acacctctga ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat
    17941 tcaagtgcca attacaataa tttagatatt tatcaaatta acagcaccta ttatagtgca
    18001 ttaggcgata atgaccgtag ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg
    18061 attccacaaa tttattacgt tgggttatta gcaggaaaaa atgatattga actgttggaa
    18121 gcaacgaaag aagggcgtaa tattaatcgt cattactatg atttagccga aattgaagaa
    18181 gaagtgcaac ggccagtgat tcaacaatta tttgcacttt taaaatttag aaatgaatct
    18241 gcggcgtttg atttggatgg aacgattgaa gtagcaacgc catcagaatc agaaatagtg
    18301 attacacgta aaaataaaaa cggtagtgat agtgcaacat taacggctaa cttggatacg
    18361 aaagaattta tcattgagtc taaagatata aaaattttca ctagtgttgc attaaaattt
    18421 taacctattc catagaacaa aaaatccttt atactagcac ttggatgact tatccaagac
    18481 caataaaaag gactcataca tggataagta taaaacaaat acagcattta ataaatggtt
    18541 ttctgccata aaattaggaa aactttctgt caactcccaa aaatgtattt ttgattacaa
    18601 ttcgtatcgt aaaaaactgt cttttgaagc tgtgctcaag ctctttctct acgcagttaa
    18661 tgaagaaaaa gaaagtttgc gtgatttgag tacagctctt atcgaccgat cgctccaact
    18721 agaaaccgat gtgtcatcga ttagtcatac ccaattgtct cgagcattta atgcacttga
    18781 acctaaagtg ttagaagaaa tttttcaaca actgttagaa aaagttcaac atcaaatcaa
    18841 accaaccaag cgaaatcatc tgtatctgat tgattccagt acgttttcgc ttagtttaaa
    18901 acgtcataag tgggcgactt tccgtcaaac aaaatcaggt gtcaagttac acttaaacta
    18961 ttgttatatg gacgatacta cgatgtatcc aactgatttt accttaacaa atgcccatga
    19021 acatgatctc aatcaactac cagtattggt gaatcaacca gaggcaactt acgtgtttga
    19081 tcgtgggtat taggactttg aaaagatgga tcagatgcat tgggatggct atttcttcgt
    19141 cactcggatt aaaaaaaaca ccaaagttca cgtgatagat actctggaaa catcgccaga
    19201 aacagaaatt ctacgagacg agctggttcg tttaggatct aaaacctatc taaccgccaa
    19261 tttccgacta gtcactgttc aagataagaa cggacgtgtg tttcaattta tcaccaatcg
    19321 aatggacgtc tcttcaaaag aaatctctga tatgtatcac gctcgttggc aaatcgaatt
    19381 gtttttcaag cacatcaaac aacacatgac cattaaaacc ttcttttctc aaagtgaaaa
    19441 aggcgtgcag aatcaattga ttttaacgat gatttccgct ctcctcacgt ttctcatcaa
    19501 attggagacg aaaacggaaa aatcagtgtt ccaaatcaaa cgattctttc gttatttact
    19561 gtttcagccc tttgaatgtt gtctagaaaa attaattccc acatgagaaa aaagaaattt
    19621 ggctcgttac ttttggtttt cagttgaata acagataaaa aattttctgg aaaaagtcat
    19681 cctctgcgtc agcacagttt tttttgactt attttcagag gaaatagttt tcagaatatt
    19741 ctaaaatgtt ttatgcaaca ctagtgatat tttttagaat tagatttcta atgtgcattt
    19801 tttgagtatt aaggagcaag tatacaaaga tttataaagc tgattaaatt tgaataaaaa
    19861 tatgcaggaa cgctactatt tttagtagtg ttctttttta tatttgaata aaaaatgtgg
    19921 tggttaaatg gtggttataa caaaaaatga attaatgaaa ttaggttatg ggaagtatca
    19981 gtctgagaga atcatacgac aggcaaaagc aattatggtt tcaaaaagct atacctatta
    20041 cgaaaataga agattaggac tagtacctgt tgaatctgta gaagaagttt taggtatgaa
    20101 gatagaggta gaggaggatt ttcagaatca tggttaattt taaaaaagat aaaaatggga
    20161 aatatcattt tactgctagt ttgggattcg acaaacgaac agggaaaaga gtacaaaaaa
    20221 acgtagaggg gttaaaacag ttaaagaaga aaaaaagcgt ataccgaact tgtttctaat
    20281 tatggaaaag aagcagaaac ttctcaaagt actatgttgt ttgaggagtt tttttataaa
    20341 ttattcctac cttattatca agggaaagta gaagaaagaa catataaaaa tcggttatct
    20401 acaatcaaaa aaacttttct tatttcttta aaatggagtt aagagaaata cgtccaattg
    20461 acattcaaaa gtggcaaaat aatttattga gcaaatataa aaattcttat gtaagaaaca
    20521 tgtatgggat ttttcagatg tcattagatc gagcagttgt tttgggacta cttgaaaaaa
    20581 atcctgcaaa agttattgga aatgtgaaga aaaggaaaac tacagttgat ttttggacaa
    20641 aagaagaatt tacaaaagtt atttcaactt tttatattga tgatttgtac caacattttg
    20701 gttttatttg tatgtggtta ctttttatga ccgttatgcg tttgggagaa gccactgcat
    20761 taacttggaa agatattgat tttgaaaata aaaacttgcg tgtgaataaa acactctatt
    20821 ataagagagc caaaacctat gagtttgtag aacccgaaac ggcttctagt attcgtacaa
    20881 tcgaactaga cgacaataca attacctttt taaaagcttg gaaggagcgt caagtttcaa
    20941 taggaggcat gtcgtttgta ctttcttata atggaacacc tactcagaaa tatacaatcc
    21001 gtcatattat gagaagacat gcaacgatcg ttggtgttca tataatccgc atacacggat
    21061 taaggcatag tcatgcttct ttgcttatat cacaataaaa agatgcactt cgaattaaag
    21121 aaaggttaaa acatgctgat gtacaaacga ctttaggaac ttatggtcat ttgtatctaa
    21181 attcattgag agagataaca aatgatccca cagattatat ccaaactaat ttttctaaaa
    21241 tcaaaccgac acctatttac gataataaaa aataaatgat aaacaaaagt aggatcacac
    21301 caaaagaaac acactagaaa aactataata caggcttttc tagcgtgttg tttcttattt
    21361 ccgcctattt atacattata ctcataaagc atttaatgca tcgatcgtag ccgcattgtc
    21421 tatatgggta tataaagttg ttgtattggt acttgaatgt cccaattgtt gggcagttaa
    21481 taccaatgaa tttgtctgtt ggtagagtct agtagctaat gtatgacgca gtttgtgagg
    21541 gctgacttga attctaaagc cttttgaata ttttgctacc atccgttcga ttgcaatacc
    21601 ggatatacgc ttggcttctc cttggtaaat tgctagaaat aaagcttttt cgttttctgt
    21661 tactttatat cgttctttcc gaatagctag ataattagct aaatattcca ttgcgaatgc
    21721 tgctatattg acagcatctc ttttatttcc ttttctagtt actaccacat tattcttatc
    21781 taaagatagg tcatctaaat taatatttat aacctcggag agtctcaatc cagatcctaa
    21841 aatcagtgca ttgatcgcca ggtctctttc tacatctcgt tgataataga tcaaagctcg
    21901 tttactcaaa gtatttttat attcattggc aatataatct agataatttt gtgtatcctt
    21961 atctagaaat aattttttct caatggcatt ggcacgtgca gctaacgtag ttttatctgt
    22021 gatggttgct acttgatcca taggattatt gtctaagtat ggttttccta gaggattatt
    22081 ggaacttttc gaaagatatt taaatagaac ctttaatgcc gtgatatttc tattgatagt
    22141 agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg tctctttttt
    22201 tacattttct tttgtgcgac gtttcaaatg agatttgtac aagtttaggt cataggcagt
    22261 taactgtctt atcgaggtga tagaagtatc aggaacgata gactcactca gaagccattc
    22321 ataaaagagc ttataatttt ttagatattc taaagtggtt cgaggtgatt gatctttatc
    22381 ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga ttgtatctat
    22441 tttttgatat atttctgtat ttttcatgtc attcatcctt tcaataagta ggggagaaga
    22501 gtatctctat aattggacta taccaattat agaatgtttt gtcaattggt atgctctaaa
    22561 atttattaat ttgtttcaaa aaaagcatat aaacaggtat acgtttaata atttaaaata
    22621 aatggtatac ataaataggg aaggaaatag aagggagcaa tctaaatttt ttgaattttc
    22681 ttaattaatt atatagaaag aacaatcttt ctatagagtt agacatgaga gggactatgc
    22741 ttgatttaaa atcaacaagc gtatatactg tatatacaaa gaaaaaggag ggagaagact
    22801 atggcaatta aagaaagaaa atttagggaa gtggggaact cagtagtatt aactatttca
    22861 aaggagtttc ttaaagaaaa tgggttaaat cccggagata cggttttttt agatgaagaa
    22921 aaacttcgag cggcaattac aaaaaaacaa gccttatctg atgaaattga tttagcaata
    22981 tctcaagcat taactgaata tgatactact tttagagagc ttgtcgataa atgagtattc
    23041 attacctaga agccaaagat attataaaaa tgaatgtaat ccaaataaaa aaatactctc
    23101 ctaatgaacc ccttggtgta aaggatgcga atgctttaga aatgtgtgta gggcaacttc
    23161 gagcctcagc ttttggtgaa gaggtttatc cttcgattta tgaaaaagca gctatcctac
    23221 ttatacaatt gataaagaaa catccttttc acaatgcaaa taaacgtact gctttcttag
    23281 ctacttttgt ctttttgaaa atcaatggac agcttttagc tattgatcaa aaagatgctg
    23341 taaatttagc ggtatatatt gctacgtatg ataaggattt cgatcaacta aaatatgaag
    23401 tgatcaacac tataaagaca catacgcagc ccatttaagg agttactgta tgtgtcttta
    23461 tagggttatt agctgctata gcgaaataat tgaagtaaat tacaaatagc tataattcaa
    23521 atccaactat atagaaaacg taaattttct atatagttgg attaagataa cgactattta
    23581 ttttatcata tttttactat accctatact tttacttggt atatttatga ataatcgcaa
    23641 atttacccct atactactag tttgtaagtt agattaataa aaatactaaa gttatttgtt
    23701 cttttattaa atacacctac aacttataaa ttgatggtat ttagataaca cgaaagaata
    23761 aaaataacag ctgatagtaa agtttgttga cgattgctac tcaatcgaaa gtgagttagt
    23821 gcgtatgaac ttgcttctga aaacttatga aatgaaagac cttttacatc ttgcagaaat
    23881 cggtactggg attaaactaa gcaataaaaa ctaatgttcc cctcattctt tgaacttcta
    23941 ctatttgatt tatttattct gataaatgac gatttcacct tcaggatctt caaatcccgt
    24001 atagaattta tttaagatct cttcagtatc agtaatagga taggcgatag gccacatacc
    24061 ctttgatggt tagaataaac tgctcaaaat ctgtatcgta tgttggcttt agcatattaa
    24121 caaaggctat ctccaaatta taaaccataa gcgttgaatc gttcttaaca atttcaattc
    24181 ttcctaaccc aaagtcagaa gatataatta ccggacttgc cttacccttc atgtgggttg
    24241 ttgagattcc atatttaaag ggaattgtcg gtactatcgg cggtctgaaa aacatcccaa
    24301 aaataaaaaa atctttcccc tgctagtctc ctaagtccct ttgtagcgga tatggcacca
    24361 taatctcatt ttatcgaagt gagtcatggt ttatctgttg gccaaccaaa attgatacaa
    24421 tctaacgatg caccctctac ggcattctag gggagtgcac ttgcattcac ccaataaaaa
    24481 ccggcagtta gcaaagaact tatgtagtcg tatgttctta ctgttaacat acaagaggtc
    24541 tcctttccac aattgcttag cagtaaacgg gaatttacaa agctaatctc ttccattttc
    24601 ttatctttgt tctaaaagtc ccaaatggag caactgtatt tacatgaata aatttatata
    24661 cttcccatac agccgttttg gttgcctcgt ctgcccattt tcttttgtgt gcagtgaata
    24721 gatcttcatc actcattgaa tcaatcatca tgttaatatc atcaatatta cgattgagaa
    24781 tttcctctaa ttctttcaat ggtagatgtg cataggtatt tgtaaaccac ttatataact
    24841 ctcctaactg attccattta aattgatcag aaggcgtttt aacagataat ccatttcttt
    24901 catcattttc ccattttaga attaaattcg tccaaccaac ttgatatgct aaattttcag
    24961 caggtgttct atccacttcc tcaactcttt tatccttcaa ttcttcagga atagtttcaa
    25021 attctaaaat atatttagta taagtcctat ttatttcttt ttttaattct tctttatttt
    25081 tatatgttct cattatatta cctccataaa ttcctaattg tctcctttaa ttcatctata
    25141 atttcatcca cgatatttta atccgttcta caatgcatat ctgatatgca ttgactgtct
    25201 aactcatatg tgaatgattt ataaaatatc ccaacgtctc atctaaagaa atgttgatcg
    25261 tcagcaagga gctaaaaaat acgaatatat ttcttttgta gtaaaatcgg actggtttgc
    25321 atatcatttt cttcgtaaat atcaaaatca tgttaactac tatcaattac taataaaaac
    25381 taccacttta agcgttaaga actgttaagt ttagttaagt gaggatagtt cacaaaatcc
    25441 attgttaaac ttcacgaaag tggtaaaact caaaatgctt tatataggat tacagcatcg
    25501 ctctttcttc gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg acgatgacac
    25561 gttccttacg gctcatcaga tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa
    25621 tttaattcta aaaaagcgat tgccatattc acgcctcgct cgacgaacta ttaaaggtca
    25681 agatttacct gaagatcatc ctttttagga aattgaagct ttttatgttg cacaatgcat
    25741 ttataatact acaatgatgt tttcaccaga tattcttcta gggtgcggtg taatgaaaca
    25801 gatacacttg caaaagaaaa ttcaccgcga atttactaaa attatgaata attatgttaa
    25861 tcttccgaaa tttttttcca aaattaaggg atcaagctgg tgttatagat tgtttaaggt
    25921 tggcaaaaaa ataacttctt tttaaacaat tatagaaaac cgatactctg ttaagttggt
    25981 tgacacggtg tcgattttta tgactttctt attctgaaac ttcactaacg gacagtacct
    26041 aaaaagtcta tagtatggac gtttacgagt actattttat caaaaatgct gctcatttca
    26101 acttatatgt tttagtgaac tacccccact tagc
//
LOCUS       DAYYDS010000035        25919 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000035 DAYYDS010000000
VERSION     DAYYDS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.035"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..565
                     /locus_tag="ACHA02_001409"
     CDS             143..565
                     /locus_tag="ACHA02_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8669759.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(731..1924)
                     /locus_tag="ACHA02_001410"
     CDS             complement(731..1924)
                     /locus_tag="ACHA02_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8669760.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2148..2525
                     /locus_tag="ACHA02_001411"
     CDS             2148..2525
                     /locus_tag="ACHA02_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8669761.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2513..2851
                     /locus_tag="ACHA02_001412"
                     /pseudo
     CDS             2513..2851
                     /locus_tag="ACHA02_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2985..3428
                     /locus_tag="ACHA02_001413"
     CDS             2985..3428
                     /locus_tag="ACHA02_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM8669762.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3689..4504
                     /locus_tag="ACHA02_001414"
     CDS             3689..4504
                     /locus_tag="ACHA02_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8669763.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4514..5176
                     /locus_tag="ACHA02_001415"
     CDS             4514..5176
                     /locus_tag="ACHA02_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8669764.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            5384..7048
                     /gene="alsS"
                     /locus_tag="ACHA02_001416"
     CDS             5384..7048
                     /gene="alsS"
                     /locus_tag="ACHA02_001416"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8669765.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            7061..7771
                     /gene="budA"
                     /locus_tag="ACHA02_001417"
     CDS             7061..7771
                     /gene="budA"
                     /locus_tag="ACHA02_001417"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8669766.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            7901..8398
                     /locus_tag="ACHA02_001418"
     CDS             7901..8398
                     /locus_tag="ACHA02_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8669767.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            8583..8843
                     /locus_tag="ACHA02_001419"
     CDS             8583..8843
                     /locus_tag="ACHA02_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669768.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(9205..9444)
                     /locus_tag="ACHA02_001420"
     CDS             complement(9205..9444)
                     /locus_tag="ACHA02_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669769.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            9730..10044
                     /locus_tag="ACHA02_001421"
     CDS             9730..10044
                     /locus_tag="ACHA02_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669770.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(10478..10844)
                     /gene="ssrA"
                     /locus_tag="ACHA02_001422"
     tmRNA           complement(10478..10844)
                     /gene="ssrA"
                     /locus_tag="ACHA02_001422"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11015..11788)
                     /locus_tag="ACHA02_001423"
     CDS             complement(11015..11788)
                     /locus_tag="ACHA02_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8669771.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            11932..12282
                     /locus_tag="ACHA02_001424"
     CDS             11932..12282
                     /locus_tag="ACHA02_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8669772.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            12263..12979
                     /locus_tag="ACHA02_001425"
     CDS             12263..12979
                     /locus_tag="ACHA02_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8669773.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            12979..14427
                     /locus_tag="ACHA02_001426"
     CDS             12979..14427
                     /locus_tag="ACHA02_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8669774.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(14497..15006)
                     /locus_tag="ACHA02_001427"
     CDS             complement(14497..15006)
                     /locus_tag="ACHA02_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669775.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(14996..15721)
                     /locus_tag="ACHA02_001428"
     CDS             complement(14996..15721)
                     /locus_tag="ACHA02_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669776.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(15944..18064)
                     /locus_tag="ACHA02_001429"
     CDS             complement(15944..18064)
                     /locus_tag="ACHA02_001429"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8669777.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            18294..19472
                     /locus_tag="ACHA02_001430"
     CDS             18294..19472
                     /locus_tag="ACHA02_001430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8669778.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            19488..20246
                     /gene="aroD"
                     /locus_tag="ACHA02_001431"
     CDS             19488..20246
                     /gene="aroD"
                     /locus_tag="ACHA02_001431"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8669779.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            20269..20754
                     /gene="ybaK"
                     /locus_tag="ACHA02_001432"
     CDS             20269..20754
                     /gene="ybaK"
                     /locus_tag="ACHA02_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8669780.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(20825..22078)
                     /locus_tag="ACHA02_001433"
     CDS             complement(20825..22078)
                     /locus_tag="ACHA02_001433"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8669781.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            22196..23545
                     /gene="gdhA"
                     /locus_tag="ACHA02_001434"
     CDS             22196..23545
                     /gene="gdhA"
                     /locus_tag="ACHA02_001434"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8669782.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            23657..25003
                     /locus_tag="ACHA02_001435"
     CDS             23657..25003
                     /locus_tag="ACHA02_001435"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8669783.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(25311..25697)
                     /locus_tag="ACHA02_001436"
     CDS             complement(25311..25697)
                     /locus_tag="ACHA02_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8669784.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(25746..>25919)
                     /locus_tag="ACHA02_001437"
     CDS             complement(25746..>25919)
                     /locus_tag="ACHA02_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8669785.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         8582 a         4467 c         5165 g         7705 t
ORIGIN      
        1 aatgattcat atcaatagaa tacttaggaa aaaaagagaa ataataacag gtttatgcac
       61 tattgacggt gtgacaatat gatatttgta tccgtttttt tcttgtatgt agtatagtaa
      121 agtagataca ggaggagaaa atatgcagac ttttttaaca aaagtttctc aaaagaaaat
      181 taaaatgatt catttactgc ttgaaacaga acgttggtgt tcaatagaag aactacaaaa
      241 cgaattgaag gtatcaagta agtctatctt gaattatcta acagatttag aagaattgtt
      301 ccaacaatat cctgataagg tcattttgaa gaatgagaat aatcggcgct tttctatgga
      361 aaagcaggag aacttcccaa tctacattat ttatcttcat ttctacagaa aatcatataa
      421 ttatcatttg attgaattca tgtatcaaca tcctgagaaa aatttggaag attatgcaga
      481 cgctcaatat acgagtgtat ccacagtttt tcgttatgct aagttattag taaagtactt
      541 tgaaatgacg gacgtcaaag aatagtcgcc gccagataga aggaaattag cagttgaaaa
      601 agctgccctg catcaagtaa tacgaccagc caaacggaaa cagtgtctca tgtttttgat
      661 ttggctggtc ttattaccgc agatgcaagc tttttccaca cctctttctt gtcatttaca
      721 gactcaacta tcaatgctgc aagctttccc ttaatgctgt aacaccagta agagttgtca
      781 agaccagcag actgactagg taaataccgc tcaaaaacaa gattacggca gttaaactat
      841 aatgatccat caagaaaccg atgacgatag aagaaaaacc accaacagct cgtccaaggt
      901 tcataacgaa attattcgcc gtcgcgcgga tttctgttgg atacaaacta ctaatcactg
      961 cgccatatcc accatacatg ccattgataa agtagccgac tatcacagcg gctgctacca
     1021 aactccactg gctatgggct aacacaagta gatagacaga taaggatgaa caaattaaaa
     1081 acaaagaaaa ggctagttta ggccccaatt tatccatgat tatgccaaaa gtcatcatcc
     1141 ccaaagacat gcctaaaata gtactgacca tccacaatga agacccagaa accgaaagcc
     1201 ctaatcgatt ttgcataatt gaaggtagcc aattcattaa cccaaaatag ccggcaattt
     1261 ggacagttac catgatacta aggccacatg tttgccaagc aagatgtttt gattcaaaca
     1321 gtctgctaag tttggttttc ttttcggcag attgtacaga tttgaattcc tttggttcag
     1381 tcaagccgcg acgaatgaac aagaccatca acaccggcag tacacctacg acatacaaca
     1441 tcttccaacc aaacgttgga atgatcacag cagctaaaat cgcagccaaa gccgcaccga
     1501 cctgcgcacc aataccagca accgaagaag ctcgtccaat ttgttttttt gaaaaacttt
     1561 cggaaatcaa ggacatacac gccccatatt ctcctcccgc tcctacacct gcgataaagc
     1621 gaaagagata aaccaagtag atgtttgaag caaaatacat caataaagaa gcaatcgaaa
     1681 acagaaggac cgtttgagaa aatactttca cacgtccata tttatcagct atgatgccaa
     1741 aaaaaatacc tccaatgagc atccccaaat tagtgatcgt tgaaatgaaa cccgcttgcg
     1801 taccactaat atgaaaagaa gtaatcattg aagataatga aaaagctaaa aacataatat
     1861 ccatattctc taaagcaatt cccgccgatg tcgatactaa tacctttcgc tgatacgttg
     1921 tcatttttat accccttttc ctaaaatttc cctaaattgt taaaagtata tccaaaaaag
     1981 aataaaaaaa caagactttt ttatttgaaa taatacacaa acaaccgata tagataatcc
     2041 agcttagttt taatagaaat gagtattgac ttggagtaca cttaaagttg tagaataaat
     2101 aaaatacata agtaaagtgg caagaaatta agagaaagag tgatcagatg aatatcaaac
     2161 aagtcagtga agaaaagggc atttcggccg atacattgcg ctattatgaa cgaatcgggt
     2221 tgattccacc agttaatcgg acaaaaggcg gaatccgtga ttatacagaa gaagacctaa
     2281 aatgggtcga ctttacgatc tgtatgcgaa atgccggatt atcgatcgag tctttgattg
     2341 aatatattcg cctatctagc gaaggagaag caactgtatt tgagcggcgt aagcttttga
     2401 ttgaagaaag tgaacttttg acgaaaaaaa ttgctgaaat gcaagaatgt ttggaacgtt
     2461 tacaaggaaa aatcaagaaa tatgatcgtg tgttatctag aaataaaatg gaatgtatca
     2521 agtaaaaaaa tcaaggagca tcgctatgga gttacgctta ctaaaatatt tttggacaat
     2581 tgcagaagaa ggaacaatct ctcaagccgc agaagttcta catcttacac aaccaacctt
     2641 aagccgacaa ctgaaggagt tagaagagta actaggaaca gaactgtttg tacgcgaaaa
     2701 ccgcaaaatg atcttgacag aagcaggtta ttttctaaaa aagccgagca gaagaaattt
     2761 tagatttaac gaatcaaacc actcaagaat tcgagaacag aaaaaaacaa ttattcagcg
     2821 gccgatttac gatcggctgt gtggaagctg atagttcgaa tacattggca ctattactag
     2881 aaaagttgat ccgtgattat cccagcgttc aatttcatat ccacagcgga acaagtgatg
     2941 atatcacgga gtgactagaa aaaagcctgt tagatttagc gattttgcta gatccagtag
     3001 atacagagaa atacgaaacg attttgttac caagaaagga aaaatgggga ttgctcattt
     3061 tagaaaattc tgttttagca aaaaaagacg tgataacaaa agaagacttt gcgaatgtac
     3121 ctcttttgat ctcaaagcgt ccagaaatac agcgaatgtt tgctgactgg ttaggcataa
     3181 aaaaggagca gttgctgatc acaggaacgt acaatttgat tttcaatgta tttgcgctaa
     3241 ttgagaatca ggtaggtgct gctttagtca tagaaggtgc tgtaagtcat cattcaaagc
     3301 gagtgacatt cgttccgctt gacccaatga ttgaaacgaa ctgcatgcta gtctggaaaa
     3361 agaatcggat tttatcacct gtagtcaatg aattgattca ccgtttcaaa gaagcttttg
     3421 aacagtaata agtatctcta atcgaagaaa tttcaataca aaagcgagac gtatgacagt
     3481 cttgcttttg tattttttca tttttaaatc aaaaaaataa agagatttac ataacttgtt
     3541 aaataagcgc ttacaaaaat aagaagaacg gaaatgaatc agctataata accgttgttc
     3601 tggattgtaa tcattacaaa gaaatgaaga ttataacttt ggaggtgttc gaaatgaaag
     3661 aaaaaaagca aaaattcgtt caaaaaacat gccctttatt caatggttgg gtatgattct
     3721 gctgattgta ttagctttat tgacgatcat tcctttttta tggatggttt ctgcttcatt
     3781 caaagcaaat aatgaagtgt tcacgattcc gattcaatgg atccctaaaa cttggcattt
     3841 agagaattat ctggttattt ggtctcgtat tcctttagtg acatttttta agaatacagc
     3901 ttttttaagc gttgtgatca cgttgattca gttgtttact tctagttttg ctgcttacgg
     3961 gttttctaaa ctaaatttta aagggcgcga tattttattt ttagcatata tcggcacgat
     4021 tgctgtccct tggcagtctt atatgatccc gcagttcatt atgatgagaa aagtgggttt
     4081 aacagatacg ttgtggtcgt tggtgctatt acaagcgttc aatgcttttg gtgtgttctt
     4141 gttgaagcaa tattacagta gtatcccaga ttctttatgt gagagtgcgc ggatcgatgg
     4201 cttatcagag tggggaatct atcgcaagat cattttacct cttacaaaac cggcgattgc
     4261 cagtttgacg atcatcacat tcgttaatac atggaacgat tatatggggc cattcattta
     4321 tttgtcttct acggaaaata aaacaatcca gttagggttg aaaatgttta ttgggctatt
     4381 tgatgcagag tacgcattaa tcatggctgc ctcagttgtt tctgttcttc ctgttgcttt
     4441 tatattttta gcgatgcaaa aatattttgt agaagggatc gcttcatcag gaatgaagga
     4501 ataggaggtg attatgaaag agcagcgttt tgacaaaaat gtttatatgg aattctttta
     4561 ttggttgtat caatcattgg caagtagcct ttgtttttgg tgtgtcaata tcttgtatat
     4621 agcagctgcg ttccttttgt cgatagatgg aaggaatgtc ttttccttta ttttttcttt
     4681 actttttatt ggtccaggtt gcatcgtgat gataggtatg acagaagaat tagcaaaaga
     4741 aaatagtttt tcaccagtac gtttgttcat aaagatgttt cgccaatttt ggctaaaggg
     4801 atttttttat tggctatttg cttggatagt tagtgtgata atgatttttg attgtttctt
     4861 ttttatacgt ttttcttatg gaaaatggtt gattccgttg tttgttttac ttgcttgttt
     4921 atcagtttcc tttagcacca actgctggta ttttcaggtg agaaatccag cgtcgaaacc
     4981 aaatcaagtt ctacggatag cgttctacta tacgttgaaa aaatggtatg tgagtttact
     5041 agatttttta ctattgacaa gtttgtttct cttctttttc gtaaagcccc aatggtgtat
     5101 tctactaggt ccttctatcg tatttggttt gatttacttc aacaatcgta aattgatgag
     5161 aactatggat ctgtagactt tataaattga aactttcaat taagaatgac agtttttttt
     5221 atgtaaaaac tgtcattctt tttcattttt tgaaacatat ggctaaacaa atgaaataaa
     5281 acgtgctata cttttgtatg aaacaggttt agaaactgta tcacttattt gtgattattt
     5341 ctaactaata attaaaaata aagcataaaa aggacgatgt tcaatggctg aaaaaatgaa
     5401 acaaggttcg aaatctgtag tagaaagttt gattaatcat catgttgatt atgtattcgg
     5461 tataccaggt gcaaaaatcg atggtgtatt taatgaatta gaggatcaag gtcctgaatt
     5521 aatcgtgact cgtcacgaac aaaatgctgc atttatggca caagcaatcg gacgtattac
     5581 aggagagcca ggagtagtta tcgcaacaag tggtcctggt gctagtaact tagctacagg
     5641 tttggtaaca gcgacagcag aaggtgaccc agttttagca attgccggac aagtaaaacg
     5701 aagcgatcta ttgaagttga cccatcaaag catggataat gcagcactgt tccagccaat
     5761 cacaaaatac agtgcagaaa tccaagatcc agaaacaatt tcagaagtga ttgccaatgc
     5821 ttatcgtatg gctaagagtt caaaaaaagg cgcgagcttt atcagtatcc cgcaagatgt
     5881 agtggatgca cctgtccaag gtaatgtcat caagccattg agcgatccta aattaggttc
     5941 tgcttctgca gatgatatcc gttacctagc tgaacgcatt agagaagcaa aactaccggt
     6001 actattagta ggtatgcgag gatcaagtga aaaagaaacg ttagccatca ggcaattagt
     6061 tgaaaagaca gcattgcctg ttgtagagac attccaagca gctggagtga tctcacgtga
     6121 attagaagcg catttctttg gtcgtgtggg tttatttaga aaccaaccag gagatatgct
     6181 tttgaaacga agtgatctag ttattgcaat tggctatgat ccaatcgaat atgaagcaag
     6241 aaactggaat gcggaaaaag atgcgcggat cattgtgatc gatgaagcgc cagctgaaat
     6301 cgatcctttt atgcagcctg aaagagaatt gatcggtgat atctcagcca ctcttgattt
     6361 attgacaggt tcattagagc ctcaacaagt gtcagaagat gcaaaagaat atttagcatc
     6421 tcttcaagca aaattaacgg agcgggacat tgttcaatca aaaggagaag caggtatctt
     6481 gcatccttta gaagttatta acacgcttca gtcaaaagta acagatgata tgactgttac
     6541 cgtagatgta ggtagtcatt atatttggat ggcacgccac tttagaagtt atgaacctcg
     6601 tcatttgtta ttcagtaatg gtatgcaaac gcttggtgtt gcattgccat gggcgatttc
     6661 tgctgcattg gtgcgtccaa atacacaaat cgtatcagtt tccggcgatg ggggattttt
     6721 attctctgcc caggatttag aaacggcggt acgtaaaaaa ttgaatatcg ttcatttgat
     6781 ttggaatgat ggacactaca atatggtaga gttccaagaa aaaatgaaat atcagcgggc
     6841 gtcaggtgta gactttggtc cagttgattt tgtgaaatat gcagaagctt ttggcgctaa
     6901 aggaatccga gctacaagtg tagaggaatt agaaaaagct ttagaagaag gatttgcgac
     6961 agaaggaccc gtaattattg atattccgat tgattaccgt gataatgaga aattgggaga
     7021 aacaatctta ccagatcaat tctattaagg aagtgtcgac atgactgaaa aaatcttata
     7081 ccagcacggg acattaggag ctttgatggc cggcttgatg gatggaacag aaacgattgc
     7141 tcatatatta gaaaaaggaa ctctgggatt gggaacattg catgggcttg atggtgaagt
     7201 catcttttta gatggcgtag cttatcaagg aagatcagat ggctcagttg cccaattaga
     7261 tggaagtgaa cttaccccct atgcagcaat tacagatttt acgccagata ctagtttttc
     7321 tgtctcaaaa actgcagatg gagaacaatt gaaaaaagac atattagtaa gagaagccgg
     7381 ggaaaaccta tttttggcag tgaagataac tggattattc aaaaatatgc acatccgtat
     7441 catgccgaag caagaaaaac cttacagaag actagcaaaa atttcagagt ctcaaccaga
     7501 attccaacaa agcaatatcc aaggaacatt agtcgggttc tttacgcctg aattattcca
     7561 aggagttgct gcagcgggat ttcacctgca ttttatcgat gatacacata catttggcgg
     7621 acatgtaatg gattttgaag tggctgaagg aaaagttgag attagccgca tctcgtccct
     7681 tgtacagcat tttccagttg atgatccgac ttttgttcac gaaacgattg attatgctga
     7741 tttagcagca gaaatccaac aagcagaata gaaaaagaca gcgagatgcg ttcaaggtga
     7801 ttaacctgac gtatctcgtt tttttgagga ggaaacctag cgtttacaaa aaaactgagc
     7861 tataatcttc tccatagatc aaaaaggagc ggtttttatc atgtggacag aaaaacagaa
     7921 aaaagatgca tacgaaattt tattattaca gcaacaagga ttattagaag cagaagataa
     7981 ctggttagca aatttagcaa attcatctgc attgttgaat gagactttgc ctgagactgt
     8041 ctttgcagga tattatttat tcgatggtaa tgaattgatt ttaggaccgt tccaagggcg
     8101 agtttcttgc acacgtatca agatgggaaa aggagtttgt ggagaatcag ctgaaaaaca
     8161 agaaacattg atcgttgatg atgtcaaaac ccatgcaaat tacatttcct gcgattcagc
     8221 cgcacaatca gaaatcgtag tgccaatggt aaaagaaggt aatttaatcg gtgttttaga
     8281 tatcgattgc ggagtaacga aagggtatga tgaaatcgat caagcatttc tagaaaaata
     8341 tgtagatctt ttattagcaa attttaatat aaatgctttt tcatttatta ataactaaaa
     8401 tcgtctattg aaacaaaaaa gaagagaaat caagaataaa aagagccgga aagacagtgg
     8461 tcatttctgt ttttcggttt ttttattttt ttataaaatc acaagtgtta ttgattttca
     8521 caatcatatt ttaatggtat tatcattttg aataatgaaa aaacattaag gatggatgaa
     8581 atatgaataa cgaaactatc attttaggaa aaaaatatat ttgccaacct attggcttga
     8641 aacaaaaagt ggttggagaa gtaatcagta aattagaaaa ttgtgttgtg atctgcgtag
     8701 acgaatatca tgacgtcgat cgcaatgaaa tattagaaaa atgcggaaaa gtagtggcaa
     8761 aatatactca cgtttatgag ttggaaacaa cagaatatct atataactat agcaacaaat
     8821 atgaacatac tttagtttca taaagattta aataaagtgc cttcaaaagc gagaagacca
     8881 agcttttgaa ggcactttat ttagttggca gtgattgttc atgatttttg atttattcaa
     8941 aaggttcgcc tagagaatct gtctctgtaa cgaggcctgg tggagaaact cgataaaaac
     9001 ctatactgcc agaactgtcc tattttcgga tagacaaact agtagtttta aaaagatcat
     9061 ccgatccaat catttcaaaa aatgcaaaac tgatatcagg atcatttagc tcaggcttgt
     9121 tatcgtaaag catttgtaaa ggttgcattt agttaggagt aaacgcgaaa ctatcatcat
     9181 tatttgctga agaagatgaa gaagctaatt cagagtttgt ttgaaataaa ggagacgtgc
     9241 tgacagtttc tttaccctgt tttttgcaga ggtagaaact tttatatcgg aagtttctac
     9301 cggagaattt ttctcatttg tagaaattgt tgaatcgata ctattcgaaa tttgcgtatc
     9361 tgaaggtatg ttctttgatg cagaagtccc acaagcagta agtaacaata aatttgataa
     9421 actgataaac agaattcttt tcattatgta cacactcctt atagtatcgt ttagcataca
     9481 cctatgctca gtattaggca aaatcaatgc aaaactatca agtcttttat agtatctatt
     9541 agtcttagta atacatatat ggagagggtg atatcccagt tcaaaccaga tacattggtt
     9601 accatctgta ttaacgatta tgcctctaaa cgcttttttt atgaaaaaat ttataagtga
     9661 aaacgtaggt atgatattat ctagaggtaa tttttttggg tatttaaata gttgctagga
     9721 ggatagcttt tggattttta cagtaatttt atcttaataa tagcaatatt attacttcta
     9781 aatatttggt tttttgataa gagtagaaat gcagggatag gttttagaac caaacgaagt
     9841 acgtcttctg aaaaaaagtg ggtatattca cagactattt tttacggagg ggttatttca
     9901 atcagtttac tttcttcaac actttattct tttaatgtta tagacgtttc catgtcaaat
     9961 tttatatcta ttattggaat ccttatttct gcaattatta cacaactact tcttgtgttt
    10021 gaagagaagt caaaaaataa ttaaagcaat tatgcctttt agtacaggaa ttattacttc
    10081 ttacaaatgt atagcgaatg tgaagcctgc tgttttaatt gttctagaaa aacttttgag
    10141 tgaacaatta ttttatgtta ggatgaatta atttctctag agtttataat taaaaaactt
    10201 atttgaagga acatgcttaa accataattg tgtttttcct tccatcagca taaaagttca
    10261 cctcaaaaag agtgtacgaa caaaagttct ttttgtaaac cttaaaatag attttcctga
    10321 atacgtattg gggatttcta aatgtttgaa tgtttttttg aatgcaaaat caatgcaaaa
    10381 aattttgtat ttctctactt ttctctcctt gaccaaaata aaaaaacgtt gatttaacaa
    10441 cgtttttcat acatgtttat ctgtctcaac catacatata tggagacggc gggagtcgaa
    10501 cccgcgtcca aacacatcgc cacttaaaaa tctacgttca tagtcaactc atttaaggat
    10561 tcactttaca gcttgccgag tgacaggcat tctgtatcgc tagtctgatg ttctcttttt
    10621 caacttacag acggaaagtt gaaacgtatc ccactaaagt taggaccctt actcgagcac
    10681 atgggcgatg ccgagaggat ctacgctaac tgtttttagg cagctaaagc gtaagagttg
    10741 ttttcgtttt tagcagttat atttaactgt aacgttttta cgtagacgta acctacgaaa
    10801 cgcaattcaa gctcgactgt gcctgtcgaa tccgtaacgt ccccgttagc taacaagggt
    10861 tacgtgtatt tttcatacaa aattagtata gcatatattg ggataaattc aataaaaaag
    10921 cgaatcatat cccattattt atataataac tgcccctttc ttaagaaaaa ataagcgatt
    10981 ttcttaagaa aagaggtagc ttcaaaaaaa gatcttaaat cagatcaaaa tacttcaatg
    11041 cgtggacaat tccgccttct gtatttttct ttgtgataaa ggtagaaagt tcttttaatc
    11101 cgtcataggc gttgcccata gcgattttgt tgtcgcaagc ttccaacaag gcaaaatcat
    11161 tcggaccatc gccaaatcca aaagtaggaa ctttttccag ctgaagttct ttttgcagac
    11221 gtttgacacc tgcccctttt gagattcctt tcttcactgt atcgatcgag taaggaccat
    11281 tccgataaaa agttagctca ggaaaatgtt cgtgatagta ttcgtcacct gattgagata
    11341 agatcagcaa catatttact gtgttttctt taaagcctaa aggatcaatt tccggaacat
    11401 ctgaatgaat gaagtcgtaa gcattgatca ttgtttggct atgaccggta caccagattt
    11461 tctgatcgtt atagtaagaa agttcatgac cgttcaactg tacatgatca tacatttttt
    11521 gacattcttc tgaagaaaat gtttgagaat agatttcttc gccgtctact cgaatgaaag
    11581 aaccattcat ggtgaccgca gaagtaatgc ccgcatcttc catgatagag tggatttctg
    11641 cttctgtacg tcctgtagcg atgacgggga gaacattgtt ttcttttaat gcgttcatag
    11701 cttttgccac ttcgggtgtg atttttgatt tcgcatctag taatgttcca tcaagatcaa
    11761 agaaagtgat tgcacgataa tttgacacaa ataaaactcc ttttttattg gaataagttt
    11821 atcttaacaa agaatgaaca aaagacaaac ctttcgtgaa gaattcctga acatattggt
    11881 tcagtcaaca atttagagta tactataaga agagaattta ggagggaaag aatgattttt
    11941 ttcagtattg gtatcttatt cttaataatt ggcagtcttt ttcggattct gccctcaaaa
    12001 ggaaatctgc ctttttatgg gtatcattcg cctcttgccg caaaaacaga cgctcattgg
    12061 cggctggctc aaaagacaag cggaaactgg ttttttctta tgggcttact gatggcattg
    12121 atcggctatt atttaaagac atctgggcat acgaattatt ttctgattga aatgcttctg
    12181 ctagtatttc caattatgcc tatttttatt atgacagaaa agaaattaca gaaatatgac
    12241 ctggaaactg gagggaacga taatgaatat tttaatgatt gaagataatc aatctgtttc
    12301 tgaaatgatg cagatgtttt tcttaaatga aggttgggaa gcaactttca agtatgatgg
    12361 aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat tgggatatga ttacactaga
    12421 cctgaatttg cccacactgg acggtatgct agtaggaaga gagatccgca aagtttcaag
    12481 tactgtgccg attatcatgc tgacagctag agattctgaa agcgatcagg tgatcggctt
    12541 agagatgggg gcagatgatt atgtcacaaa acctttcagt cctctgacgc ttattgctcg
    12601 aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca agagaagaag cagcagtttc
    12661 agatgatcat tttgatatcg agactagtca tttcaagatg aatacgaaaa cacgagaagc
    12721 gtatctggac aaccggccaa ttgaaggact gacgcctaaa gagttcgatc tattgtttac
    12781 tttagcaaaa aaaccacgtc aagtattctc tcgggagcag ttgcttgaat tagtctggga
    12841 ttatcagtac tttggtgatg aacggacagt ggacgcccat atcaagaaat tacgacaaaa
    12901 aatcgaaaag gtcggaccac aagtcatcca aactgtttgg ggagtcggct acaaattcga
    12961 tgattctggt gttgcctaat gcgttatttg tatcagcaac tcttagcttt ttgcggaatg
    13021 gtcgcgttga tcttattgat cgttggagtt tcttttacac aactgacaaa acaaacgatt
    13081 gaagaaaaca attaccagca actgtttgga tatgctgaat cagttgaaaa gacaacgcaa
    13141 acctatgccg atacgctccc gcagtttagt caagatcagg cgtttcaaaa tgcattgctg
    13201 ctgacagagc aagtactgac agaacaaaat gtgaacttta tatttatcga taaaaacgaa
    13261 cgtgtcattt atccgactaa tacagcttta ctgaatttta ccttaacagc tgatcaatgg
    13321 aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga ataaaaatat tttaggacaa
    13381 gcacaggcga cctcttacgc acttgtgcca tttaatttga atcatgaatt ctatggagca
    13441 cttgtagtta gtcaacctgc gcgaaatatc gataacagcg ttcgtccaat tacactgaac
    13501 ctgttcaaag gatttatttt ttccagtatc gttgcagtta ttgccagcta tgcttttgca
    13561 gcattccaag ttaagcgtat caatcggtta aggaatgcga cgaaagaagt gacgaatggt
    13621 aattttgatg tacagctccc cgtacatgac aaagatgaat ttgatgagtt agcagatgat
    13681 ttcaataaaa tgacgaattc attgaaagaa tctcaagcag aaattgaaga acaagaaaat
    13741 cgtcgaagac aatttatggc agatgcttct cacgagatga gaacacctct tacgacaatc
    13801 aacggacttc tggaagggtt agaatacaat gcaatccctg aaaatcagcg agaaaacgcg
    13861 attaaattga tgaaaaatga aacagagcgt ctgatccgtt tggtgaatga aaacttggat
    13921 tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta aaaaattcaa tggaacagaa
    13981 acattgaaaa atattgttgc acagttagaa gctaaagcag aagcagcagg agatacattg
    14041 acattgaaag ccgttgatga tattgatgtt tacgctgatt atgaccgatt tgtccaaatc
    14101 atggtcaata ttattcaaaa cgcgatacag tttactgaaa acggccaaat cacgattact
    14161 ttagaaaaag gctatcttga aacgatcatc acgattgaag atacgggaat tggaatgtct
    14221 gaacaacaaa tgaaaagtat ctgggatcgt tattataaag tagatccttc aagaaaaaat
    14281 acaaaatatg gtgaatcagg attaggttta ccaatcgtgc agcaacttgt acgcttgcat
    14341 aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa caaaattcgc tgttcgtttg
    14401 cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta taaaagatca tgaatcgatc
    14461 ttctataccg cttgttttta ctataaaatg attgatttag cccgcgggct ctaaacgatc
    14521 aatataagga ctttgagcaa tagtagcatc atgagtaaag agtaagatac cttgattttc
    14581 ttcatgagct aaataagaaa gcaagtggag tagctcttgg atctccaata cactaagttc
    14641 atcaaatgta tcttcaataa caatattgtt tcgcttggaa agcaaggcat gaagtaattg
    14701 cagctttact ttttcaaaag acgtcaactc agctgccgca caattaagca aggaaggaga
    14761 gatatcaagt ttttcttgat aggccaaacg atcttgtttg atttcttttt ccggtacatc
    14821 caacaggaca tggtctttaa gattcagata aggaaacatc ggccaatttt tttggatgat
    14881 cgcaaaatta tccagttttg caacaagaac ctcttttttc aagtgctcca atattttatg
    14941 ctgtttttcg agaggacaca aaataacggt caagtgttca gcatgaatca aatattcagg
    15001 tttcattacg tccaggcctc ctactttgca aatgatggat aaaaaaagtg aaagggaata
    15061 gactaagaat gagacagcat ccactgagcc caataagagt ttgggcaaat cccttaagta
    15121 tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa aaaagtctgt tgatccatcg
    15181 gaattgtaag tccgcttacc gaagtcaaga tatcacggat atctggcagt tcagtagtct
    15241 ttaagacaag aaaattgatc ctttgaagaa gcgcttcata aactgactgg aagcttacga
    15301 tccacaataa aaccaagata caacaaagaa agagaggaaa gaacagctgc cagaaaataa
    15361 aaaggaataa tttggtttta gaaagaccag tcaggcgcca attgatcaat tcctgcttgt
    15421 tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa ggcaaataga aaaaataaac
    15481 tgaatatgcc ttttatataa aatcggctag ctaattctaa agccggaaga agctctgtag
    15541 atgcagaagc gccaataaat gaaagccgat cttggacttg attggctaaa gccgattgca
    15601 aatggtaaag agtcaggcat gctatcaata aaaaagcaga aaagcttgaa aataaacaat
    15661 agagtattgt ctgctttttg taataaatta atcgagcagt agcatagcgg attattttca
    15721 tacttttcac ctctataaaa agtatagaaa atgaatatga ataaagtatg aaattccttt
    15781 agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa agcaagaggt tgtgaagaag
    15841 ctgccctatt ctattaccgt agatgcaagc ttttgaacac tgtttattcg aaaagaggag
    15901 cagcgacaat aattttgtcg ctgctccttt taaatttatt ttgtcattcg gaagaacttg
    15961 tggtcgttcc gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc
    16021 cgttgtgttc actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta
    16081 atttctctaa gccattcttg tagtcataat cactggtatc aattgggtct aatccactat
    16141 tcgtatagaa ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat
    16201 caactttttc tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag
    16261 cttcaccagt ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa
    16321 aatcaccatc acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta
    16381 actgaatata attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa
    16441 cttgtccgcc gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat
    16501 aagggacacg ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa
    16561 ctaattcagc tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga
    16621 cgatgactga attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg
    16681 cggtatctaa ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg
    16741 ctaacgggaa tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag
    16801 caatgaattt tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga
    16861 aaaatggttt atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag
    16921 aagaatcaaa gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg
    16981 taccagcgtt cccatggaat accgcactag tgtaaccttg tgtttgactt aaaatatcag
    17041 gtgcagcttg gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc
    17101 caaacaatga attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat
    17161 ggaaaaagtt gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg
    17221 taacttcatg ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga
    17281 agctttctaa atggatataa atcacatttt ttcctttagc gagcccgaac aaatcgtcat
    17341 ttggtgctgc ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg
    17401 cttctgcccg cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat
    17461 tcaagccaag atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac
    17521 gatcagcttc cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg
    17581 caaaagccat tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca
    17641 gaacagtcaa gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa
    17701 tggcactttc tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa
    17761 tgaagtccgt gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa
    17821 aataaatgac catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc
    17881 caagtaataa caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac
    17941 tttcgattcc gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa
    18001 cgactagtaa agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt
    18061 tcaagataat cttcctttct gcacttctta atcacagaag tgtcatgaaa ccatgttaca
    18121 cagctcactt attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga
    18181 agtttatgct gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca
    18241 agtcctatgt tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag
    18301 taaaagtaaa aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag
    18361 aagaccttgc tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa
    18421 accaatttat cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga
    18481 gccagcagaa catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag
    18541 aaaaaagaac tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag
    18601 aaggagacgg aatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt
    18661 ggtataacga aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct
    18721 acccggaaat cgtaggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga
    18781 gccagttcat ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa
    18841 aatttgccgt ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag
    18901 tacggggatc attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt
    18961 ataccggcgc cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg
    19021 atctagcaaa acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg
    19081 tcgatcagca aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa
    19141 aacaattttt atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa
    19201 aagtctttcg cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag
    19261 cagaagacgg gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc
    19321 aaacaatgat tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat
    19381 atcgtctgcc aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag
    19441 tgtttgttta tcaaattaaa aataaccatt agaaggagcg atgcagaatg gtttgggtaa
    19501 agggattgaa tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc
    19561 gagaggaagt attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat
    19621 ggcgagtcga tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc
    19681 ttatccatga gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag
    19741 gcggagaaca agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca
    19801 caggattagt agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac
    19861 tgatagagga aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag
    19921 aaacaccagc agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg
    19981 atatcggaaa actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt
    20041 tgactagacg ggcaaatgct tttgtctcca tgcctattat tacgatgtcc atgggaaatc
    20101 ttggtaaaat ttctcgtctg gctggcggaa cgacgggttc cgttatgaca tttggttcct
    20161 taagcagcga ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta
    20221 ttcaattatt aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa
    20281 aaaatagtaa aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag
    20341 cacgaatatg agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca
    20401 gaaagccaat ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt
    20461 gttgctgtga taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg
    20521 aataaaaaag tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt
    20581 ggtggctgct cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca
    20641 gaaagctatg aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca
    20701 cctcaagata tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata
    20761 cgtataaaag aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta
    20821 tacattatgc tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg
    20881 tgatattaaa gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac
    20941 catcaatgat tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc
    21001 cagttacaaa tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc
    21061 atagttgaac aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca
    21121 tttcaagccg ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat
    21181 tgaccaagtt ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt
    21241 tggtttgtgt agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt
    21301 ctttattgac aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga
    21361 aaagtaaatc catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag
    21421 tgatgtcttg taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata
    21481 tcaaggggga ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct
    21541 tgtacccatc tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa
    21601 tggcaggagc atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt
    21661 ggatcaattt ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat
    21721 tgagcaatgc ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat
    21781 cctcttcttt taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc
    21841 attgcgcaaa cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat
    21901 caacaggacg attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc
    21961 ctgctttttt gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg
    22021 ctgccatcat cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc
    22081 cctcacctca aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa aatgttttga
    22141 gccaaaagag aaaatgagct aaaatgaggg aagataagca aaggagcgtg gaacgatgac
    22201 aaacgcaaca gaatatgttc aagcaattca agaaaagcta catcaaaagg atcaagggca
    22261 agaagaattt ttacaagcaa tcgatgaatt catgcccacg gttatgtctt ttttggacac
    22321 tcatcccgaa tatatcgaaa agaatatctt agaactttta acggaaccag aaagagttat
    22381 tcaatttcga gtgccttggc aagatgatga aggaaactgg cgagtaaata gagggtaccg
    22441 tatccaatac aactcagcta tcggccctta taaaggtgga ttgcgcttcc atcctagtgt
    22501 caatctaagt atcttgaaat ttttggcttt tgaacagatt ttcaagaata gtttaaccgg
    22561 tctgccaatc ggtggaggaa aaggcggaag tgacttcgat cctaaaggaa aatctgataa
    22621 tgagatcatg cgtttttgtc aaagtttcat gctggaattg tcaaaacata tcggcccatc
    22681 gattgatgtt cctgcgggag atatcggtgt aggagctaga gaaatcggct atttgttcgg
    22741 cgaatataaa cgattgaaaa aatatgatac tggtgtgttg acaggtaaac ctttggattt
    22801 ttggggaagt aagatacgta cggaagctac tggttatgga ttagtgtatt atgtcaaaca
    22861 tctattgaac gaagaaaaag attcatttaa tcaaaaaaca gtttttgttt ccggaagcgg
    22921 gaatgtagcc atttatgcaa tcgaaaaagt gcaagagcta ggcgggaaag ttgttacatg
    22981 ttcagattct agcgggtaca tctatgatcc agaaggaata gatgtgaaac tattgaaaga
    23041 agtcaaagaa atcaatagac aacgtttgac ggaatacgca aaccaacgtc cttctgctat
    23101 ttatcatgct ggagaatctg tttggacatt gaaagaaaaa gcggatatcg ctttgccatg
    23161 tgctactcaa aatgaaatag acgaaagctt ggccgaaatc ttagtagaaa atgggatcaa
    23221 aatcgtagca gaaggcgcta atatgccatg tacagtaaaa gctgtttctg ttcttcatga
    23281 agcaggcgtg tggtattgtc caggaaaagc tgctaatgct ggaggagtag cagtatctgc
    23341 tttagaaatg agtcagaacg tccagcgatt gacttgggaa agagagcaag tggatgagca
    23401 gctggatcaa atcatggaaa cgatttacca tacgtgtcgc caaacagcag ccgagtacgg
    23461 cgacgaaaaa aatctgcttc ttggagcaaa tgttgctgga tttgaaaaag ttgccaaagc
    23521 aatggcagca gaaggtttag tttgaaaaat gaaactttct tggaaatatt gtcagcattt
    23581 tgtttatttg cttgaaaaat aataaaaaaa aacgtaaagt attacctgta gagtaaacct
    23641 ataaggagga gaacaaatgg cacacattca ttttgactat tcaaaagtag caccgtttgt
    23701 caacgaacaa gagttagggt acatgcaaag ccaagtaaca gcagcccaca acgaattacg
    23761 ggaaggtact ggcgcaggaa gcgacttccg cggctggatc gatttaccaa cgaactatga
    23821 caaagaagaa tttgcacgta tcaaagaagc agctaaaaaa atccaatctg attctgaagt
    23881 attagttgtt atcggtatcg gtggttctta tttaggcgct cgtgcagcga tcgacttctt
    23941 gaatcacaca ttcttcaatt tattagatag caacacaaga aaagcaccac aaatcttctt
    24001 tgccggaaat tcaatcagct caacatatat tgctgacttg atcgaagtaa tcggtgatcg
    24061 cgacttttca gtaaacgtta tttcaaaatc tggaacaact actgaaccag cgattgcgtt
    24121 ccgtgtattc aaagagcttc ttgttaagaa atatggacaa gaagaagcaa acaaacgtat
    24181 ctacgctaca acagataaag caaaaggtgc agttaaagta gaagcagatg cagaaggttg
    24241 ggaaactttc gtgattcctg atgacatcgg cggacgtttc tctgtattaa ctgctgttgg
    24301 tttgttacca atcgctgtta gtggtgcaga tatcgatgca ttgatgcaag gtgccgctga
    24361 tgcaagcaaa gcatactcaa gcgacaaact agaagaaaac gaagcttacc aatacgcagc
    24421 aatgcgcaac attctttacc gtaaaggcaa agtaactgag ttgttgatca actatgaacc
    24481 aggaatgcaa tacttctcag aatggtggaa acaattgtat ggtgaatctg aaggaaaaga
    24541 ccaaaaaggc atctatcctt caagcgcaaa cttctcaact gatttgcact ctttaggaca
    24601 attcatccaa gaaggacgtc gcaatatttt tgaaacagtt gtgaaggttg aaaaacctcg
    24661 taaatcaatc acaattcctg aacaagcaga agatttagac ggtctaggct acttgcaagg
    24721 aaaagaagtg gacttcgtaa atacaaaagc atttgaagga acattgcttg ctcatacaga
    24781 cggtgacgtg cctaacttgc tagtcaaaat tccagaaatg gatgcttata cattgggtta
    24841 tacaatgtac ttctttgaaa ttgctgtagg tatttcaggt tacttgaatg gaatcaaccc
    24901 attcgaccaa ccaggtgtag aagcttacaa gaaaaacatg ttcgcacttc taggcaaacc
    24961 aggctttgaa gatcttgcta aagaattgaa cgaacgcctg taagataagg gtttttcgct
    25021 agatttaaat aatcagtgat agaaacatgt tcgctttaaa atgaaaactg gtacccattt
    25081 aggtactcaa acatactgca tcgtttattc agaataaggg gctgtgacaa gacgtttgtc
    25141 acagtccctt gtttgtatag aaatcgattg attgccttgc tctttagttt gttttaggac
    25201 tttaagaaca gtgaggctga cttcgaaggt taattctgtt tttttgtttt ctagaatcag
    25261 taagcaaaaa agcactcact tcgagtgctt ttttgcgata tattcttagc ttaagatagg
    25321 cctaattttt cttttacaga tgagggtacc ttatcgctgg taattacttt tttctctatg
    25381 atccgctttt cgcttgcttt caactgcaag atcgttcctt cggggtaagc agaaccattg
    25441 gcagagtcac cagaagtaat gacatcgaaa tctaactgtc ttttttcacc gttttcagca
    25501 taagcatcta cgttgtattt aaaacctctt ccaattactt ccccattatc tgctttgata
    25561 tcagtttctg caggcattgg agctttgact accgcataat aatctttacc tacataagta
    25621 gactgatagt agtcccaacc tttccatcct ataaaagcaa tcaatatgat aacgatcaac
    25681 cctaaaattt ttttcatgaa agatctcctt ttttattttt attatacccg tggtgctagt
    25741 taattttaat atcctaaaga atatctcaaa gttccttcaa aacgtcgttg tgctcgactt
    25801 aatagaacac tgtaagccag taatatgctt tcaaatctac tctctagccc tttaacagaa
    25861 cgtgagttga aattttgaca acctaatttt tctaaggaag aaaaaacagt ctcaactgt
//
LOCUS       DAYYDS010000036        24971 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000036 DAYYDS010000000
VERSION     DAYYDS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.036"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="ACHA02_001438"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="ACHA02_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8669786.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="ACHA02_001439"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="ACHA02_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8669787.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="ACHA02_001440"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="ACHA02_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8669788.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4744..5628
                     /gene="ftsX"
                     /locus_tag="ACHA02_001441"
     CDS             4744..5628
                     /gene="ftsX"
                     /locus_tag="ACHA02_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8669789.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="ACHA02_001442"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="ACHA02_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8669790.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="ACHA02_001443"
     CDS             7565..8422
                     /locus_tag="ACHA02_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8669791.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="ACHA02_001444"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="ACHA02_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8669792.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="ACHA02_001445"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="ACHA02_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8669793.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="ACHA02_001446"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="ACHA02_001446"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8669794.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /gene="pstB"
                     /locus_tag="ACHA02_001447"
     CDS             11178..11936
                     /gene="pstB"
                     /locus_tag="ACHA02_001447"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8669795.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="ACHA02_001448"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="ACHA02_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8669796.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            13203..13895
                     /locus_tag="ACHA02_001449"
     CDS             13203..13895
                     /locus_tag="ACHA02_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8669797.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            13892..14659
                     /locus_tag="ACHA02_001450"
     CDS             13892..14659
                     /locus_tag="ACHA02_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8669798.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="ACHA02_001451"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="ACHA02_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8669799.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="ACHA02_001452"
     CDS             16624..16944
                     /locus_tag="ACHA02_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8669800.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="ACHA02_001453"
     CDS             16944..17294
                     /locus_tag="ACHA02_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8669801.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /gene="hprK"
                     /locus_tag="ACHA02_001454"
     CDS             17461..18399
                     /gene="hprK"
                     /locus_tag="ACHA02_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8669802.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /gene="lgt"
                     /locus_tag="ACHA02_001455"
     CDS             18414..19244
                     /gene="lgt"
                     /locus_tag="ACHA02_001455"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8669803.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="ACHA02_001456"
     CDS             19288..20313
                     /locus_tag="ACHA02_001456"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8669804.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="ACHA02_001457"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="ACHA02_001457"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8669805.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="ACHA02_001458"
     CDS             21391..21831
                     /locus_tag="ACHA02_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8669806.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="ACHA02_001459"
     CDS             21834..22436
                     /locus_tag="ACHA02_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8669807.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="ACHA02_001460"
     CDS             complement(22495..23601)
                     /locus_tag="ACHA02_001460"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8669808.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="ACHA02_001461"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="ACHA02_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8669809.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT         8223 a         4340 c         5425 g         6983 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa a
//
LOCUS       DAYYDS010000037        24700 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000037 DAYYDS010000000
VERSION     DAYYDS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.037"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACHA02_001462"
     tRNA            8..81
                     /locus_tag="ACHA02_001462"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="ACHA02_001463"
     tRNA            101..173
                     /locus_tag="ACHA02_001463"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="ACHA02_001464"
     tRNA            201..274
                     /locus_tag="ACHA02_001464"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="ACHA02_001465"
     tRNA            288..361
                     /locus_tag="ACHA02_001465"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="ACHA02_001466"
     tRNA            385..474
                     /locus_tag="ACHA02_001466"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="ACHA02_001467"
     tRNA            487..560
                     /locus_tag="ACHA02_001467"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="ACHA02_001468"
     tRNA            579..654
                     /locus_tag="ACHA02_001468"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="ACHA02_001469"
     tRNA            656..726
                     /locus_tag="ACHA02_001469"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="ACHA02_001470"
     tRNA            740..813
                     /locus_tag="ACHA02_001470"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="ACHA02_001471"
     tRNA            838..925
                     /locus_tag="ACHA02_001471"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="ACHA02_001472"
     tRNA            947..1018
                     /locus_tag="ACHA02_001472"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="ACHA02_001473"
     tRNA            1041..1130
                     /locus_tag="ACHA02_001473"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="ACHA02_001474"
     tRNA            1143..1216
                     /locus_tag="ACHA02_001474"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="ACHA02_001475"
     tRNA            1235..1307
                     /locus_tag="ACHA02_001475"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="ACHA02_001476"
     tRNA            1311..1383
                     /locus_tag="ACHA02_001476"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="ACHA02_001477"
     tRNA            1388..1458
                     /locus_tag="ACHA02_001477"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="ACHA02_001478"
     tRNA            1471..1544
                     /locus_tag="ACHA02_001478"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="ACHA02_001479"
     tRNA            1556..1627
                     /locus_tag="ACHA02_001479"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /gene="metK"
                     /locus_tag="ACHA02_001480"
     CDS             1893..3086
                     /gene="metK"
                     /locus_tag="ACHA02_001480"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8669810.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="ACHA02_001481"
     CDS             3216..4688
                     /locus_tag="ACHA02_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8669811.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="ACHA02_001482"
     CDS             4771..5559
                     /locus_tag="ACHA02_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8669812.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="ACHA02_001483"
     CDS             complement(5626..6294)
                     /locus_tag="ACHA02_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8669813.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="ACHA02_001484"
     CDS             6409..8121
                     /locus_tag="ACHA02_001484"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669814.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="ACHA02_001485"
     CDS             8124..9893
                     /locus_tag="ACHA02_001485"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669815.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="ACHA02_001486"
     CDS             complement(10098..10664)
                     /locus_tag="ACHA02_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8669816.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="ACHA02_001487"
     CDS             complement(10664..11620)
                     /locus_tag="ACHA02_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8669817.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="ACHA02_001488"
     CDS             complement(11620..12276)
                     /locus_tag="ACHA02_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8669818.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="ACHA02_001489"
     CDS             12646..14664
                     /locus_tag="ACHA02_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8669819.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    14734..14940
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15091..17505
                     /gene="leuS"
                     /locus_tag="ACHA02_001490"
     CDS             15091..17505
                     /gene="leuS"
                     /locus_tag="ACHA02_001490"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8669820.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="ACHA02_001491"
     CDS             17853..19406
                     /locus_tag="ACHA02_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8669821.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="ACHA02_001492"
     CDS             complement(19787..21571)
                     /locus_tag="ACHA02_001492"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669822.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="ACHA02_001493"
     CDS             complement(21586..23307)
                     /locus_tag="ACHA02_001493"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669823.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="ACHA02_001494"
     CDS             23407..24168
                     /locus_tag="ACHA02_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8669824.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            complement(24510..>24700)
                     /locus_tag="ACHA02_001495"
     CDS             complement(24510..>24700)
                     /locus_tag="ACHA02_001495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669825.1"
                     /translation="MLEEKSLNTYNYFISKKMLSVVSPIAEESHSRDYLIKKYVYSIH
                     LFIFALLTYCNNVSNNVF"
BASE COUNT         7695 a         4504 c         5202 g         7299 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt tttgcttata
    24661 aaataattat aagtatttag agatttttct tctaacattc
//
LOCUS       DAYYDS010000038        23888 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000038 DAYYDS010000000
VERSION     DAYYDS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.038"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            231..1175
                     /gene="mvk"
                     /locus_tag="ACHA02_001496"
     CDS             231..1175
                     /gene="mvk"
                     /locus_tag="ACHA02_001496"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8669826.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1168..2145
                     /gene="mvaD"
                     /locus_tag="ACHA02_001497"
     CDS             1168..2145
                     /gene="mvaD"
                     /locus_tag="ACHA02_001497"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8669827.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2158..3243
                     /locus_tag="ACHA02_001498"
     CDS             2158..3243
                     /locus_tag="ACHA02_001498"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8669828.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3240..4304
                     /gene="fni"
                     /locus_tag="ACHA02_001499"
     CDS             3240..4304
                     /gene="fni"
                     /locus_tag="ACHA02_001499"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8669829.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4553..5356)
                     /locus_tag="ACHA02_001500"
     CDS             complement(4553..5356)
                     /locus_tag="ACHA02_001500"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8669830.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5376..6218)
                     /gene="kduI"
                     /locus_tag="ACHA02_001501"
     CDS             complement(5376..6218)
                     /gene="kduI"
                     /locus_tag="ACHA02_001501"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8669831.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(6234..7241)
                     /locus_tag="ACHA02_001502"
     CDS             complement(6234..7241)
                     /locus_tag="ACHA02_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8669832.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(7248..7892)
                     /locus_tag="ACHA02_001503"
     CDS             complement(7248..7892)
                     /locus_tag="ACHA02_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8669833.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(7992..8750)
                     /locus_tag="ACHA02_001504"
     CDS             complement(7992..8750)
                     /locus_tag="ACHA02_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8669834.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(8877..10028)
                     /gene="fucO"
                     /locus_tag="ACHA02_001505"
     CDS             complement(8877..10028)
                     /gene="fucO"
                     /locus_tag="ACHA02_001505"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8669835.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(10110..10421)
                     /gene="rhaM"
                     /locus_tag="ACHA02_001506"
     CDS             complement(10110..10421)
                     /gene="rhaM"
                     /locus_tag="ACHA02_001506"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8669836.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(10423..11268)
                     /gene="rhaD"
                     /locus_tag="ACHA02_001507"
     CDS             complement(10423..11268)
                     /gene="rhaD"
                     /locus_tag="ACHA02_001507"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8669837.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(11282..12538)
                     /gene="rhaA"
                     /locus_tag="ACHA02_001508"
     CDS             complement(11282..12538)
                     /gene="rhaA"
                     /locus_tag="ACHA02_001508"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8669838.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(12535..13995)
                     /gene="rhaB"
                     /locus_tag="ACHA02_001509"
     CDS             complement(12535..13995)
                     /gene="rhaB"
                     /locus_tag="ACHA02_001509"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8669839.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            14148..15017
                     /locus_tag="ACHA02_001510"
     CDS             14148..15017
                     /locus_tag="ACHA02_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8669840.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(15143..16042)
                     /locus_tag="ACHA02_001511"
     CDS             complement(15143..16042)
                     /locus_tag="ACHA02_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8669841.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(16207..17031)
                     /locus_tag="ACHA02_001512"
     CDS             complement(16207..17031)
                     /locus_tag="ACHA02_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8669842.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(17042..18337)
                     /locus_tag="ACHA02_001513"
     CDS             complement(17042..18337)
                     /locus_tag="ACHA02_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8669843.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(18353..18832)
                     /locus_tag="ACHA02_001514"
     CDS             complement(18353..18832)
                     /locus_tag="ACHA02_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8669844.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(18838..19866)
                     /locus_tag="ACHA02_001515"
     CDS             complement(18838..19866)
                     /locus_tag="ACHA02_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8669845.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(19883..20989)
                     /locus_tag="ACHA02_001516"
     CDS             complement(19883..20989)
                     /locus_tag="ACHA02_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8669846.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(21218..23815)
                     /gene="adhE"
                     /locus_tag="ACHA02_001517"
                     /gene_synonym="adhC"
     CDS             complement(21218..23815)
                     /gene="adhE"
                     /locus_tag="ACHA02_001517"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8669847.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         6743 a         5293 c         4332 g         7520 t
ORIGIN      
        1 tatttttttc tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt
       61 tctttagaaa acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga
      121 atgttttttg agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg
      181 cgctttattt tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact
      241 atggccaagg agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg
      301 gagaaccagc gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc
      361 tggatgcaat ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc
      421 ctcatgcatt aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc
      481 aaaaaaactt gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa
      541 gcgctgcagt cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt
      601 ctcgtgaaat actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta
      661 gtggaatcga tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc
      721 ctttcgacta cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca
      781 aaggacaaac aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg
      841 aaactggaca aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca
      901 ttgaaaattc accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa
      961 agctgacaat cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg
     1021 cattgggtgc caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa
     1081 caaaaacaaa agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt
     1141 ggatacaagg attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac
     1201 attgctctaa tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac
     1261 agcctttcgt taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc
     1321 tatatggtag atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa
     1381 gtcagtcagt ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta
     1441 atcagtcaga atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct
     1501 gctttagcag gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca
     1561 agatttgctc gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa
     1621 tgggaaaaag gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc
     1681 gaagacgatt tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc
     1741 agaaatggga tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc
     1801 gttgaagggg atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc
     1861 ggagaaacga tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct
     1921 ccattcactt actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga
     1981 aagcaaggca ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg
     2041 gaaaacagcc atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa
     2101 gtaattactg cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg
     2161 attgaagtat ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca
     2221 ggccatccag cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca
     2281 cgaaaagtcg gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga
     2341 cgcaacggag aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct
     2401 attcgcttga ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg
     2461 aaagtaacaa gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt
     2521 gccgtgaccg ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag
     2581 ttggagattt tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc
     2641 ggcgacatcg ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct
     2701 tggctccaag aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca
     2761 ggtctatcca ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg
     2821 ggtagccctg cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa
     2881 atggtggctt atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa
     2941 gggtttaaag aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta
     3001 ctgcatgatt tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg
     3061 tgtaatttag ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat
     3121 tgcggaatcg taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa
     3181 aaagcagaaa tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga
     3241 tgaatcgaaa agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg
     3301 aatttgactt cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg
     3361 atctaagtac acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga
     3421 ctggcggaag cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag
     3481 cagatttgat gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg
     3541 atacttatac gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg
     3601 cagggacttc tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac
     3661 agattcattt gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca
     3721 attggaaagt acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag
     3781 aagtcggttt tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata
     3841 cagttgatat cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct
     3901 ccaagcggga actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg
     3961 aagcaaatga agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct
     4021 atgacatatt taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac
     4081 tgacgcatct gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag
     4141 aagagcttcg tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac
     4201 aatctctgat cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag
     4261 tgaagtatgg aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc
     4321 acgctctaaa cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa
     4381 ttcacaaaaa tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc
     4441 ttctgtccca gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg
     4501 ttcaatccgc tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt
     4561 gttttccgat ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat
     4621 cagatgcgct agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc
     4681 gtgctgctgg cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt
     4741 gtgtttcgcg taatggagca gtttgcggtg tagcaatata acctggtcca attccattac
     4801 attggatatt gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc
     4861 cttttgccgc agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga
     4921 tattgatgat tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga
     4981 tgaatggggc atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga
     5041 tcatcggtgt ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat
     5101 cttctttgat ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat
     5161 aaccttttgc atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt
     5221 gattcaagtt attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa
     5281 atccaatacc atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat
     5341 ccatactaaa ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc
     5401 atatccatgt ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg
     5461 cttgtaccta cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct
     5521 acaactaaat gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca
     5581 ttggcataat caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc
     5641 gtattccaag cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg
     5701 catacatttg gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca
     5761 gtctccattg gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct
     5821 ggaacagaac taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca
     5881 tgtcgtgttt cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca
     5941 tcgatctcga tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag
     6001 aagtaatcaa cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc
     6061 actccaccaa agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca
     6121 aatacctttt ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct
     6181 tctggactgt gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga
     6241 atgatttttc cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg
     6301 cagtccccat ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct
     6361 tgcggagaca tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca
     6421 cctactcgat ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc
     6481 atccacaatg tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa
     6541 taaaacacct gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt
     6601 tgttcttctg cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca
     6661 gctgagcaga tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt
     6721 gtccataatt ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca
     6781 gcttctatca cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct
     6841 gaaacatgaa atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc
     6901 atttcagcaa aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta
     6961 ccagtttcca tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct
     7021 actccataac attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt
     7081 gtagcaaaaa tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct
     7141 ccgccataat gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac
     7201 aatcgcaaca tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt
     7261 tctttcattt ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta
     7321 gctgcgggtg ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac
     7381 tctttcatat tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc
     7441 gccttgaagg cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata
     7501 tctaccccgc ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa
     7561 ggaatttgat acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata
     7621 tactctgcgc cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg
     7681 ccaatcacta tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct
     7741 gcttgaggga ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg
     7801 caagcattca aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt
     7861 ttcaatcgag ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat
     7921 tttattttat aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa
     7981 cgcttttttc attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca
     8041 ctgattattt gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat
     8101 gcaccaatga tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg
     8161 atttcttcat cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac
     8221 cgtaatgcat tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa
     8281 tcagtgtact gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat
     8341 aatggtcggg tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt
     8401 ttgttcacgt acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc
     8461 ttttgctgca gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg
     8521 tttttattcg cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta
     8581 tttacgtagc cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt
     8641 cccacaccct ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt
     8701 attttagagg ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct
     8761 cctatttctt tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag
     8821 atataaacta ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac
     8881 atcaaacttt gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt
     8941 gtgcatacat ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct
     9001 aattcagaaa tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa
     9061 gccgcttctc ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga
     9121 gcgatctcac ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa
     9181 agtgctgcac acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc
     9241 ccgtgtacca gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat
     9301 gccatttttt ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg
     9361 atttcgattg cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac
     9421 ccttcaatag catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt
     9481 cccatcatca tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa
     9541 acaaattttc ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct
     9601 gttccagaag tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca
     9661 ccttctaaac taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt
     9721 gccgtatcga ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta
     9781 acaaaagcca ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat
     9841 aaagcataag cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc
     9901 tcgatcaatc ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca
     9961 gggacgatcg actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga
    10021 ttactcattt tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc
    10081 caagcaaccg gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa
    10141 ctgactggag aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt
    10201 tgattgatag atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa
    10261 ccaaataaca aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca
    10321 cgaagtgctg tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt
    10381 ttttctgggt aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt
    10441 atgaacttcc tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca
    10501 caggttttcg tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac
    10561 ttctgctgcc ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga
    10621 cccgtagatt ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt
    10681 tgcttcccca atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg
    10741 aaaaacaact aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc
    10801 taattcatgc gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg
    10861 attatctggg tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct
    10921 tgtaggaagg cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc
    10981 aactcgaacg attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc
    11041 tgaaccagtt acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat
    11101 gcggcggatc actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata
    11161 agaaatattc ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc
    11221 gatcatttct ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact
    11281 cctattttct taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca
    11341 aaccaactgg aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat
    11401 ctttcaattc ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt
    11461 tcaatcgctc aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac
    11521 cgatgaccca agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg
    11581 ttttttccaa caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca
    11641 tcaaaaccac atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt
    11701 tgctgaataa agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc
    11761 cagcatctag acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg
    11821 aaccgactgt ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta
    11881 cataccgttc atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc
    11941 gtggggtaaa acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga
    12001 tacttgtttg cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt
    12061 gttcgatcca gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca
    12121 tcattggatg cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc
    12181 actctaccca tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg
    12241 tatccaaata gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct
    12301 tctccagatc ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg
    12361 ccatgattcc accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt
    12421 gccagcaatg catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta
    12481 caccgatagt tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac
    12541 gattactcct ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg
    12601 gtaaaaagtt tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga
    12661 aaactgacct gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg
    12721 tcctgcttct acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt
    12781 cgatccgccc cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt
    12841 gatcagctct tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc
    12901 cgatgagaca gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat
    12961 atttggggga ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc
    13021 ttttgcagca agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac
    13081 ttcctggatc aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc
    13141 ccattcattc gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc
    13201 aatccctaaa agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg
    13261 cgttcctaat acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg
    13321 caaatcatat tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt
    13381 caatggtgga aataattcct tcgagacacc gattttttca agaagttcta tttcccattc
    13441 ttttgtccct gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt
    13501 accagtaaag acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa
    13561 attttctttt tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc
    13621 tgtctgctca tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt
    13681 ggtacgcttg tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag
    13741 acagtaatcg actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt
    13801 taatttttca aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg
    13861 accgtctttg ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc
    13921 ttttgcatcc ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac
    13981 aaatgttgga ttcatttctc cacctctttt actttattgt tgattagcac tttattaata
    14041 aagcgttatc atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac
    14101 tattttggta tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg
    14161 aaaaagccaa ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa
    14221 gaagcaggaa tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt
    14281 actatactga acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag
    14341 gttcgcttac atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg
    14401 actgccaaga atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga
    14461 tccatttgaa tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata
    14521 acaccttcca ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat
    14581 tgatccacat gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc
    14641 acgagttgct gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata
    14701 ttcccgccta cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta
    14761 cacttgatca attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt
    14821 ccaaatttat cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta
    14881 aagaccttct tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg
    14941 agaattttgc ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact
    15001 ttcgtaaatt cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata
    15061 tatagataaa aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc
    15121 ggttattttt tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact
    15181 gtttctgtgt atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg
    15241 ctttcagaaa atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc
    15301 ggtattctcg caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa
    15361 acgtaaatcc agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact
    15421 gcttttccat ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca
    15481 tttgttccac acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt
    15541 gtacaacttg attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt
    15601 caccgtaaat ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt
    15661 cctgttctat ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata
    15721 gattagacca tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac
    15781 gctcgaatac atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca
    15841 gcacacttcc tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct
    15901 ttaatgtagc atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga
    15961 cgacatcttt gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac
    16021 cgctgatttt cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta
    16081 ttataccacg ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag
    16141 agacgtggca tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc
    16201 tttacattat tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag
    16261 aaccacatac aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat
    16321 cacaccgtct cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa
    16381 atgaaccgca catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg
    16441 ttcttgataa tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc
    16501 cgaatctact gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa
    16561 acctggttct atccccacca tcatttggta tttttctcca gcttttacca agcgacggat
    16621 aacggacaca gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc
    16681 cggcaaaaca catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg
    16741 aagatactgc tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat
    16801 attgatatag caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac
    16861 ataatttccc atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag
    16921 acctaaggcg agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc
    16981 tatcgtttgg acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct
    17041 cctaactcag catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca
    17101 agacgactaa gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta
    17161 cgcctcctac acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta
    17221 cacacagatt catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga
    17281 cgattggtaa aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac
    17341 ccaccagtaa taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc
    17401 ccataatcaa cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca
    17461 tggaagaagt agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca
    17521 tattaggtat atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt
    17581 aaatgacagc tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga
    17641 taatgatgac aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag
    17701 ccatctgtcc tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta
    17761 gcatgattcc tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga
    17821 tcgaagcacc accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca
    17881 tacctgtcgg agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt
    17941 ccacttgctg agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag
    18001 agatggaacc aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc
    18061 gcccgacgaa caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga
    18121 taatgcccga gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca
    18181 ccattttttg agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca
    18241 tcatcgatga gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa
    18301 atacaaaaaa taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt
    18361 tttcttgaat tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa
    18421 tgatgagtac ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag
    18481 aaggcgagat ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca
    18541 tcgcagcaaa taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc
    18601 cagttaattt ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg
    18661 cggaagacaa catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat
    18721 ttggatttcc taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa
    18781 caagcaaaac agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta
    18841 cgctccttgc tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat
    18901 atcatgaagt ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac
    18961 tccatgttct tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat
    19021 ttcaaaaacc gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag
    19081 ctgttctttg atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata
    19141 ggaatagtat ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc
    19201 tgatccgttg atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac
    19261 gggcgaaccg cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat
    19321 cttctttccc ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg
    19381 ctgaccagaa tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat
    19441 tggctccatt atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag
    19501 ataaggaact gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac
    19561 ttttgtgaaa tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag
    19621 ttgtccatct gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg
    19681 cccaaattca ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa
    19741 ggtaactatc tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca
    19801 gccgcttaga cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt
    19861 tttcatatgg gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca
    19921 ccgttaacga ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac
    19981 tgtcccccat tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt
    20041 caagcaatcc ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt
    20101 tatggaccgc tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag
    20161 aagaaggatc atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg
    20221 cacgcacttg tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata
    20281 attctaagat ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc
    20341 aaaatgcttc ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg
    20401 ttcgtttatc catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat
    20461 attctgggcg attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg
    20521 tatcgtccca caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt
    20581 catttgtccg aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat
    20641 agcgtgaatc tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat
    20701 tgacgttttt gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt
    20761 gataagcgcg aggatttttt gtcatctggt aatttttata aataccatat agaccgacac
    20821 cttgtggcca attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca
    20881 acaaaaattc accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt
    20941 tttctttcac taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac
    21001 ctttttaaac gctttcattt tatatcctta atatagcagt aataccgtcc actataaata
    21061 tcgctttttg aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt
    21121 tcagttactt ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc
    21181 atctattttc ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga
    21241 tgatctgttt caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt
    21301 cgtaagctaa ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt
    21361 gagcttttag actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta
    21421 aagcttcgac taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg
    21481 cgtaatctgt atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat
    21541 cttttgcatt atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga
    21601 tcccgtattc cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag
    21661 caaacgccat accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag
    21721 gtgtttttac tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc
    21781 cacgtgtata atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat
    21841 ccatcccggt atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga
    21901 ttgcgacatc tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt
    21961 cagtgatgac cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata
    22021 cgaattgtgt tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt
    22081 tttgttttgc accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag
    22141 ctgcgtccat tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca
    22201 tcagtttcgt tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga
    22261 cttcgatctt cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac
    22321 cgaattggac cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct
    22381 ctaagtacaa tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca
    22441 tgttatttct ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac
    22501 gagaaacaga gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt
    22561 tatagatatc accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt
    22621 tcattcgaag accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat
    22681 gtcctaatcc tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt
    22741 tagctttgat catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc
    22801 ctacgccttc caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca
    22861 attttgcgat ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt
    22921 cgttcatcac tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt
    22981 gtgcttggaa ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt
    23041 cagaagcaca aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac
    23101 gtttaatttt tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag
    23161 ctggttttcc tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga
    23221 tcgcgatacc tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc
    23281 attggataca attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg
    23341 cttctgcaga agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt
    23401 tcaacgcgat cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc
    23461 cgcaaaccac accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga
    23521 tcacaccaac tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat
    23581 ttttgattgc tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta
    23641 atggcatatg ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt
    23701 cttgatcaaa gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa
    23761 tcattgcttg tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta
    23821 tcctcctaat gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta
    23881 tttttcta
//
LOCUS       DAYYDS010000039        23831 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000039 DAYYDS010000000
VERSION     DAYYDS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.039"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACHA02_001518"
     CDS             complement(55..789)
                     /locus_tag="ACHA02_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8669848.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACHA02_001519"
     CDS             1116..1526
                     /locus_tag="ACHA02_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8669849.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACHA02_001520"
     CDS             1519..2220
                     /locus_tag="ACHA02_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8669850.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACHA02_001521"
     CDS             2220..3806
                     /locus_tag="ACHA02_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8669851.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACHA02_001522"
     CDS             3825..4046
                     /locus_tag="ACHA02_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8669852.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACHA02_001523"
     CDS             4043..4804
                     /locus_tag="ACHA02_001523"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8669853.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACHA02_001524"
     CDS             5184..5693
                     /locus_tag="ACHA02_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8669854.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACHA02_001525"
     CDS             complement(5768..6307)
                     /locus_tag="ACHA02_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8669855.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHA02_001526"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHA02_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8669856.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACHA02_001527"
     CDS             7320..8171
                     /locus_tag="ACHA02_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8669857.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACHA02_001528"
     CDS             8199..8834
                     /locus_tag="ACHA02_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8669858.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACHA02_001529"
     CDS             8854..9627
                     /locus_tag="ACHA02_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8669859.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACHA02_001530"
     CDS             10035..10832
                     /locus_tag="ACHA02_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8669860.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACHA02_001531"
     CDS             10810..11223
                     /locus_tag="ACHA02_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8669861.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACHA02_001532"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACHA02_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8669862.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACHA02_001533"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACHA02_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8669863.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACHA02_001534"
     CDS             16000..16560
                     /locus_tag="ACHA02_001534"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8669864.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHA02_001535"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHA02_001535"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8669865.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACHA02_001536"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACHA02_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8669866.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACHA02_001537"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACHA02_001537"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8669867.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHA02_001538"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHA02_001538"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8669868.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHA02_001539"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHA02_001539"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8669869.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACHA02_001540"
     CDS             21907..22485
                     /locus_tag="ACHA02_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8669870.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACHA02_001541"
     CDS             22537..23019
                     /locus_tag="ACHA02_001541"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8669871.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACHA02_001542"
     CDS             23136..23510
                     /locus_tag="ACHA02_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669872.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DAYYDS010000040        23422 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000040 DAYYDS010000000
VERSION     DAYYDS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.040"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..1677
                     /locus_tag="ACHA02_001543"
     CDS             16..1677
                     /locus_tag="ACHA02_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8669873.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1747..2094)
                     /locus_tag="ACHA02_001544"
     CDS             complement(1747..2094)
                     /locus_tag="ACHA02_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8669874.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2178..3119
                     /gene="opp3b"
                     /locus_tag="ACHA02_001545"
     CDS             2178..3119
                     /gene="opp3b"
                     /locus_tag="ACHA02_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8669875.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3120..4163
                     /locus_tag="ACHA02_001546"
     CDS             3120..4163
                     /locus_tag="ACHA02_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8669876.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4169..5239
                     /locus_tag="ACHA02_001547"
     CDS             4169..5239
                     /locus_tag="ACHA02_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669877.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5229..6182
                     /locus_tag="ACHA02_001548"
     CDS             5229..6182
                     /locus_tag="ACHA02_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669878.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6194..7204
                     /locus_tag="ACHA02_001549"
     CDS             6194..7204
                     /locus_tag="ACHA02_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8669879.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7489..8310)
                     /locus_tag="ACHA02_001550"
     CDS             complement(7489..8310)
                     /locus_tag="ACHA02_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8669880.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8407..8685)
                     /locus_tag="ACHA02_001551"
     CDS             complement(8407..8685)
                     /locus_tag="ACHA02_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669881.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8953..10962
                     /gene="metG"
                     /locus_tag="ACHA02_001552"
     CDS             8953..10962
                     /gene="metG"
                     /locus_tag="ACHA02_001552"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8669882.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11441..12028)
                     /locus_tag="ACHA02_001553"
     CDS             complement(11441..12028)
                     /locus_tag="ACHA02_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669883.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12203..13525
                     /locus_tag="ACHA02_001554"
     CDS             12203..13525
                     /locus_tag="ACHA02_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8669884.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13680..14456
                     /locus_tag="ACHA02_001555"
     CDS             13680..14456
                     /locus_tag="ACHA02_001555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8669885.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14449..15018
                     /gene="rnmV"
                     /locus_tag="ACHA02_001556"
     CDS             14449..15018
                     /gene="rnmV"
                     /locus_tag="ACHA02_001556"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8669886.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15011..15895
                     /gene="rsmA"
                     /locus_tag="ACHA02_001557"
     CDS             15011..15895
                     /gene="rsmA"
                     /locus_tag="ACHA02_001557"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8669887.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16346..16975
                     /locus_tag="ACHA02_001558"
     CDS             16346..16975
                     /locus_tag="ACHA02_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669888.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17099..18208)
                     /locus_tag="ACHA02_001559"
     CDS             complement(17099..18208)
                     /locus_tag="ACHA02_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669889.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18417..18839
                     /gene="mgsA"
                     /locus_tag="ACHA02_001560"
     CDS             18417..18839
                     /gene="mgsA"
                     /locus_tag="ACHA02_001560"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8669890.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19026..19124
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19162..19698
                     /locus_tag="ACHA02_001561"
     CDS             19162..19698
                     /locus_tag="ACHA02_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8669891.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19738..21501)
                     /locus_tag="ACHA02_001562"
     CDS             complement(19738..21501)
                     /locus_tag="ACHA02_001562"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669892.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21504..23219)
                     /locus_tag="ACHA02_001563"
     CDS             complement(21504..23219)
                     /locus_tag="ACHA02_001563"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669893.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7540 a         4293 c         4775 g         6814 t
ORIGIN      
        1 aaaggggtac gtaaaatgaa aaagaaattt acatttggtt tggttgcggt atgcggcctt
       61 gtacttgctg gatgttatgg cggcagttca gccacaagca gtagttcagc aaaaagcggt
      121 tcagcagatg gaggaggcgt atttaactta gtcgtgcctc aagaaatgcc tacagctgac
      181 ttatcagttg caacagatac aatcagtttt acagcattga ataacgttta tgaaggaatt
      241 taccgtcttg atgaagacag caaaccacag ccggcaggag ctagcgagtt ggcagaagtc
      301 agtgaagacg gattaactta caaaatcaaa ttacgagaag acgcaaaatg gtcaaatggt
      361 gatcctgtta cagcagcgga ttacgtttat ggctggcaac gaacagtaga cccagctaca
      421 gcttctgaat atgcttacct gtatgcgcca gttgaaaatg cagaagacat tacagctgga
      481 aagaaagata aatcagaact agggatcaaa gcagttggtg actatgaatt agaaatcaaa
      541 ttgacgaaac aaacaccata tttccaatat ctgcttgctt tcccatcatt tttcccacaa
      601 aaacaatccg tagtggaaga aaatggggat gcctatgcat ctgcaagtga taaagctgtt
      661 tataatggac cattcacatt agaagatttt gatggaccag gaacagatac agaatggact
      721 tataagaaaa atgatcatta ctgggacaaa gatacagtaa aactttctga aatcaaagtc
      781 agtgtagtaa aagaatcttc tactgcattg aacttattca aagatggcca agcagatgac
      841 gtgatcttgt ctggtgagtt agcacagcaa aacgcaaacg atccagctta tacttctgtt
      901 aaagaagcgc ggacaagcta tatcgaattc aatcaacgtg aaaagaattc accattcaac
      961 aatgtgaacc taagaaaagc tatttcttat tctatcgatc gtgaagcttt agtaaaacaa
     1021 gtactaggtg acggatcagt tgtctctaca ggattgatcc catcagatat ggtaaaaagt
     1081 ccaacaacga acgaagactt tgcaaaagaa gctggaaaat tagtttctta tgacaaagat
     1141 aaagcaaaag aatattggga aaaagcgaaa aaagaattgg gcgttgattc tttagaattt
     1201 gatttgatgg cttctgatga tgattcatcg aaaaaagtaa tcgaatatgt tcaaaactca
     1261 atccaagaaa acttggatgg cgtaacagtc aaaccaacac ctgttccatt ctctgtacgt
     1321 cttgaccgct caacttcggg tgattttgac gcagtactag gcggatgggc tgctgactat
     1381 gcagacccaa gcagctttac tgatttgttt gtcacaggta attcatacaa ccgtggtcgc
     1441 tggagcaatg cagaatatga cgaagcagtt aaagcagctt catcaacaga cgctggcgat
     1501 gaacaagccc gttgggatga tttccaaaaa gcaatcaaga tcatctctga tgatatgggt
     1561 gtcgttccgg tgtaccaaaa agcagaaggt cacctagtga atgaaaaaat caaaggtatc
     1621 gttcaccatg cggcaggcgc ttcatgggat tacaaatgga catacgttga agaataatcg
     1681 ttaataccgt gagcatgaga aaaaacgagg ctgcggcaaa tgtcgcaacc tcgttctcat
     1741 attcacttat cctaacaaag aaaaaagcat gaaaatagca gaaagagcaa ttagtattcc
     1801 ggcaatgatc agccaaaagc tgtacatacc gtagctggca gacgagagcg aggaaaattc
     1861 tggttttttc tttcggttcc ttgttcgtgc aagatggacc gatcgctgaa aatgatcgaa
     1921 gaaacccgaa gaaaagaccc ataaaaaacc gccgataatc agaaacggca atgaccataa
     1981 gaatagatca ttagataatt gaatgagtgt aaaggaatgg ttgatgagat tttttattaa
     2041 aataaccaga atcagtagaa aagctactgc gtaggggata gatttttttt tcatttgatt
     2101 gctcctttat ttttggttca ttatctaata atgtagcgaa acatctgtca gaagtcaaaa
     2161 gctaggggga aacattagtg aatagttata tcaaatatgt attaaaacgt gtctttttca
     2221 tgatcatcac gctttggtta attgcaacaa tcaccttttt cttgatgcaa ttgctgccgg
     2281 gcacgccata tacgaatcag gaacgtttga gtcctgaaac aatcgagatg ctaaataaac
     2341 aagtcggttt ggataaacca gttatcgtac aatatgggat ttatctttct aatttgcttc
     2401 aaggagattt tggaatttcc ttccaattta aaaaccagcc agtcgcacat ttgttagctg
     2461 gaagaatcgg accatccttg cagttagggt tacaggcaat tatctttgga acagtcctag
     2521 ggacgatttt aggaacgatc tctgccatga agcaaaatac atgggcagat acatcttcta
     2581 cactcgtagc gatattaggt cgttccattc caaactttgt atttgctgtt ttattacaat
     2641 atatctttgc aatcaaatta caagttttgc caatcgcaaa atgggatggc tttgtgtaca
     2701 caatcttacc aacaattgcg ttggccatgt cgccgcttgc tgactctgct cggtttatcc
     2761 gtacggaaat ggtggaagta ctgcatagtg attatgtgga attagcaaaa gcaaaaggat
     2821 tgagccgttg ggaaatcgcc tttaaacatg gtcttcgaaa tagtttgatt ccattgatga
     2881 ctttattagg accgttagct gtagcattga tgaccggatc actggtggta gaaaatatct
     2941 ttgctattcc tggtatcggg gaacaattcg ttaagtcaat cacaacaaat gattatccta
     3001 cgatcatggc tgtaacgatc ctttactcat ttatgctgat ttttgttatt ttgatcgtgg
     3061 atctattgta cggtctagtt gatccaagaa ttcgtgtttc agaagggagc cgaggataaa
     3121 tggaaatgat accaaagcgt tatgaaacag ttgcagatat ccctgcggat gaatttcttc
     3181 cattacaaaa aaataccgaa gaagaacgtg aacagattac tgcaccttct ttgaatttta
     3241 tccaagattc atggcgccgt ttaaagaaaa acaaagctgc cgttatttca atggttttac
     3301 tattggtcat cattttgatc tctgtgatca caatctttgt ttctcctcat gatccaactg
     3361 cccaaaacgt ggcttatatc aatttgcctc ctcgtattcc gggaatcaat atcgatggat
     3421 tgaatggaaa agcaatggtt ggtggtgaac ttgttgataa atatgctcaa gcaaacgtcc
     3481 cttcaaacgt aaatttcttt ttaggaacag acggtttggg acgtgatgta ttaagccgct
     3541 tatttatggg aatgagaatc tcgttgctga tcgcatttat tgctgcatta ctagatgtaa
     3601 cgattggtgt agcttacggg ttggtttctg gtttgttagg cggacgtgtc gataacgtga
     3661 tgcaacgttt tttggaaatc ttatcaggaa ttccaaacct cgttgtcatg attttgatgt
     3721 tggtcgtttt tgaaccagga attttttcaa tcgtggctgc tatggccatc acaaactgga
     3781 taccaatggc acgtatcgtt cgtgcacaaa caatgaaatt aaaagatcaa gaatatgttt
     3841 tagctggatt gacattaggt gaatcaagaa tgaaaattgc attcaaacat attttgccga
     3901 atatctctag tgtcattatt attcaaatga tgtttagtat tccatctgct attttcttcg
     3961 aagccttttt aagctttatc ggtttaggtc ttacgcctcc gtcagcttca ttaggaacga
     4021 tgttgagcga agggtacaag acattcctgt atctgcctta tctattatgg attccggcag
     4081 taactttatc ggttatcatg attggcttta acttacttgc tgatggattg cgtgatgcct
     4141 ttgatcctaa aatgaaagag tgaagacgat gacgaaaatt ttagaagtga aagacttaga
     4201 aatctctttt gatacgtatg ctggaaaggt acgagcgatt cgcggtgtca attttcattt
     4261 gaataaagga gaaacattgg cgatcgttgg ggaatctgga agtggaaaat cagtgacgac
     4321 cagaagcatc atgcgcttgc tgtcaagcaa cgccaatata gatgctggtc aaatcctctt
     4381 taaaggtaac gatatcgttg gaaaatcaga aagagagatg caaaaaatcc gtggtaaaga
     4441 aatagcgatg attttccaag atccaatgac ctctttagat ccgacgatgc ccattgggaa
     4501 gcaagtcgct gagtccctta gaaagcataa taaagtttca aaaaaagagg gacaaaaagc
     4561 tgcattagaa ttgcttaaat tagttggaat acccgaagct gagaaacgta tcaacagtta
     4621 tccgcatcag ttctcaggag gtcagcgtca acggatcgtg atcgcgattg cactggtatg
     4681 ttatccggaa atcctgattg ctgatgaacc tacaacagcg cttgatgtga ctattcaagc
     4741 acagattttg gagctgctga aagaaattca gacaaaaatc gatacatcaa ttatctttat
     4801 cactcatgac cttggtgtag tagcgaatgt cgctgatcgt gtcgctgtca tgtatggagg
     4861 aagaatcgtt gaagttggaa catcggaaga aattttctac aacccgcaac atccctatac
     4921 ttggggactg cttggttcaa tgccgacatt agatagtgcg aacgatcgat tgtatgctat
     4981 tcctggttct ccaccggatt tattgaaccc gccaaaagga gacgcattct atccaagaaa
     5041 tgaattcgcg atgaaaatcg atgcagaaca agaaccacca ttttttgaac tatcaaaaac
     5101 acacaaggca gcaacttggc tacttgctcc gcaggcaccc aaagtgacac cgccagaaga
     5161 aatccaacgt cgctgggcta tctttaaaga gaaacaaaaa catacttacg gtcaaggagg
     5221 aattgctgat gccaaataaa ctacttgaag ttaaaggatt gaaacagtat ttcaatgtag
     5281 gaaaaaagaa tgaagtacat gctgtaaatg acatcagctt tcacatttat gaaggagaaa
     5341 cattcggttt agttggagaa tctggaagcg gaaaatccac tactggccgt acgatcattc
     5401 gcttgaatga acctactgat ggtgagatat tattcgacgg tcaagatata acgaagatca
     5461 aagataaaaa aggtttgacg aaattccggc atgatgttca gatgatcttt caagatccat
     5521 acgcttcgtt gaatcctcgt atgaaagtca gagatatcat tgccgaagga attgatgtta
     5581 atggactagc taaatcatct gaagagcgtg caaaaaaagt cgatgatcta ttgagaactg
     5641 ttggattgaa tccaagccac ggtactcgtt atcctcatga attttcaggt gggcaacgcc
     5701 agcgtattgg tattgcacga gcattggcag taaaaccgcg ttttatcatt tgtgatgaac
     5761 caatttctgc actagatgtg tcgatccaag cccaagtggt caatttgttg caagatctcc
     5821 aaaaagaaca tcaattgacc tatctattta ttgcacatga tttgtcgatg gttaaacata
     5881 ttagtgaccg aatcggtgta atgcataacg gcttgttatt agagatggga acaagtgatg
     5941 aaatctataa tcacggtgtt catccttata cagaaagttt gttatctgct attcctctac
     6001 cagatccaga ccatgagaga aaacgcagaa gaatcaaata tcaacctgaa ccagatgacg
     6061 gacaagtacg tcaacttcgt gaaatcgcac cagaacattt tgtctatgca actgagcagg
     6121 aagtcccata ttacgcaaaa aaactaaaac gacaaaagga atctcttctg gttgctaact
     6181 aggagggaca tagatgctaa aagcttttaa attccgtatg tatccgacag aagaacaaaa
     6241 acagcaattg attcgtactt ttggatgtgc tcgatttacc tataaccatt tgctgaaaaa
     6301 gcggcagaaa agttggcaac aaacaggagt agccaatttt tcgctcacac ccgctacatt
     6361 aaaaaaagaa tacccattct taaaagaagt agacagtctt gctttagcaa atgcacagct
     6421 taatttggat cgggcttttc gcaattattt caagggacgt gccagcttcc caaagcttaa
     6481 aacgaaaaaa agtatgtggc aatcttatac gaccaacaac caaacccgta cgatctatct
     6541 ggaaaatggc tacttaaagt taccaaaaca aaaagaacta atcaagataa atcagcatcg
     6601 gccagttgaa ggatcgattc gctccgctac gatttctgct cgatataatg aagaatttta
     6661 tgtcgcacta ctttgtgatg tttcgccagt aaaaaaagaa tctttagcta aatggatcgg
     6721 gatcgcctat catccgaaga cattgatcca aacatctcga cctctagaag taactttacc
     6781 aaaatttcac cagacagaag aaaagctgca acatgcacag cggaaattga atgtgaaagt
     6841 ccgctccgct caccatagaa agatcaggtt agatcaggca agcaattatc agaaacaaaa
     6901 gagaaaagta atggatcttt atctgaaaca aaagaaccag cgagaagatt atcttgaaca
     6961 attatctggg aaattggtca aacaatatga ctacttattt gtagaatctt ttccaaaaga
     7021 agaggcacat gctgattttt ctattcacga ctggcataaa ttaataacaa aattgcgtta
     7081 taagtcccaa tggtataata aaaaatttct tttgatcaat accgacggag cagaagaaag
     7141 caattccgta agaaagagcc aagtagtaga aaaaatgggc agacattcgg ttatcaaggg
     7201 ataaaaaatg aatgaaactg ataggagcgt tagggttagc cttgttaata aaaagtctta
     7261 atgacttttg tttctaggaa tttttctttt gaagaaaagc tcactaatta tgacaaacca
     7321 ataaaacaat agcagaaaaa gttcgtacga gctttttctg ctattgttaa ttttttattc
     7381 agaaagttta cgaatacatc aaaattctga aacaaaaaac gtaactcaca aagagttgtt
     7441 tttgcgtttt ttcttgaatg attaggtctg aatcttttgc tcacaacctt attcatggtg
     7501 ccccggcttg agctgataga tttttgaagg acggccgatt cctacaggac gttcaccgat
     7561 ctcatcgaaa tattggagca tagctttctt aaaattggaa tgatcgatcg ttttataatc
     7621 gatccctaag aatttagcaa atacttttcg agcttccgta atcgtgaaat ctttacctaa
     7681 aacttgtaga acttgcggct cgtgttccat tttattgact acacggttga atgctttcaa
     7741 gatgatttga ctatggtcaa aagccaaagt atctttgcca gaagactctc cagtgactaa
     7801 atcaagcgag atagccactt ctccgctaga taaatggatc ttgttactat gacgttctag
     7861 tgtgaaccag cgtacttcct tggcatcatc tccggcgatt agcggttctt ctccaataaa
     7921 ggctaagtag ctgactgtga cgacccagcc tcgcggatct cgattaggcg tactgaatgt
     7981 atgcagttgc tcaatatttt cttttgaaat aaccacacca gtttcttcct ttgtttctct
     8041 taagacgctt tctccagttg actcatcctt ttggacaaag ccaccaggca atgcccatga
     8101 atttcgataa ggatgccctt ttctttggat caacaaaact ttcagctgat cttcttcttt
     8161 gttatagcac atcagtacga tatcgacagt caaagagggc ttgtcatatt ctggacgttc
     8221 ctgtttttga taccagttaa gaaattcttc ttcacttgct ttatgttcat aatataattt
     8281 ttcttcttct ttagaagcaa aacggttcat ctgaccacct ccattgacag taaaagtact
     8341 ttttacctta ttgtagcaga ctctgaaaaa aaaccaagga tttacttgaa aaatctaggt
     8401 tatcatttaa atatgaaaaa taaatggaag tatgagattg aataaaaaga aaagcagata
     8461 aaacggcaat actttatacc gctcttcacg aatggcaaag aagccacaaa taacgatata
     8521 tacgtcaatg ataaaacgca aaatccaagc tgctgtcata aaactgttca aatgcccatg
     8581 gaggttaaaa taaccataca tacgaatata aaaatagctg aatattaaat gtgctaatga
     8641 aagaaaagca atactaagtg taaaaaaaag atgtttcttc tgcatgattg ttccagtccc
     8701 ttactaagat agatagtctt tattatacca cgtgtgaggg gagaaataaa aagccgtttt
     8761 ttagtagata tgaaacaaaa caaaaacccg catttattca acctattcat ccttttaaag
     8821 gatagattga acgtagatag aagtcgttta tccagtcttt ttcattttga aaaaaagcta
     8881 acagcatgct ttttttatgg tatactagca aggaaacgaa ttcgcatcat gcgataaagg
     8941 aggaagagcc ccgtggcaga aaaggaaact ttttatatca ctacgccaat ctattatcca
     9001 agcggcaaac ttcatatcgg caactcgtat actacgattg catgtgacgc aattgctcgt
     9061 tataaacggt tgatgggctt tgatgtgttt tatctcactg gagtggatga acatggtcaa
     9121 aaaatcgaaa caaaagcaga agaactaggt gtaaaaccgc aagaatatgt agataaaatg
     9181 gcggctgatg tcaagaaatt atggaaaacg cttgatatca gttatgataa attcattcgc
     9241 acgacagatg attaccataa agcagctgtt caaaaaatat ttgatcgttt attagaacag
     9301 ggagatattt acttaggtga atatgaaggc tggtactctg tttctgacga agaattcttt
     9361 acagaaacac aacttgcaga agtgtacaaa gatgacgaag gtaacgtgat cggtggtaaa
     9421 gcgccaagtg gacacgaagt agaattggta aaagaagaat catacttctt ccgcatgagc
     9481 aaatacgcag accgtctatt ggaatactat gaagaacatc ccgaattcat ccagccagaa
     9541 tctcgtaaaa acgaaatgat caacaacttt atcaaaccag gattagagga tcttgcagtt
     9601 tcacgaacaa cttttacttg gggagtacca gtcaagagtg atccgaaaca tgtggtgtat
     9661 gtatggatcg atgcattatc caattatatt actgcactag gatatggctc cgaagatgaa
     9721 agtctattcg aaaaatactg gcctgcagat gttcagatgg tcggtaaaga aatcgttcgc
     9781 tttcatacga tctattggcc aatcatgctg atggcccttg atttgcctct acctaaaaaa
     9841 gtatttgggc atggctggct attgatgaaa gacggaaaaa tgtctaaatc aaaaggaaat
     9901 gtcgtttatc ctgaaatgct tgtggaacgt tatggtttag atgcactccg ttattatttg
     9961 cttcgtgctg ttccgtttgg gtcagatggt gtctttacgc cagaagattt tgtttcccgt
    10021 gtcaattatg atctggcaaa tgatctaggg aacttattga atcgaacgat tgctatgatc
    10081 aacaaatatt gtgatggact cgttcctgac tatgcctcac ttgtcactcc atttgatagt
    10141 gaactgtcta cgacggctgc taacgttgtc ggacgttacc atgatgcaat ggaaaaaatg
    10201 gaattcaata cagcattggc tgaaatctgg gtgctgattt ctcgtgcaaa caaatacatt
    10261 gatgaaacag aaccatgggt attagcaaaa gacgaagaaa agaaagctga attgaatagt
    10321 gtcatgatcc atttagctga atctttgcgt atcgcagcga tattacttca gccaatcatg
    10381 acagaaacgc cagagaagat cttcaatcaa ctaggattgg actctgaaac aatgaatctt
    10441 gaaggattac actttggcga atttccttca ggaacaaaag tagttgcaaa aggtacacca
    10501 atcttcccac gtttagatat ggaagaagaa gttgctttta ttcaagaaaa aatgtctgaa
    10561 ggtactcaaa caaacgaaga tacagtcaaa tgggatccag aagaaacaga acttgtttca
    10621 acgaaagaaa aacaaatcaa atttgatgta tttgaaaaag tagaactaaa agtagcagaa
    10681 gtcatcaact gtcaaaaagt agaaggggca gataagttgc tgcaattccg actagatgct
    10741 ggagacagcc aagaccgtca aatcttgtca ggaatcgctg aattttatcc tgatccaagc
    10801 gaattgatcg ggaaaaaagt cgtcattgta gcaaatctga aaccacgaaa gatgcgtgga
    10861 caaatcagtc aaggaatgat cctttctgca gaagcaccag atggctcttt acaagtaatc
    10921 gaagcaccaa aatccatgcc aaatggctca gaaatcgcat agaaattcaa agggggtatg
    10981 acactagttg tgtcataccc cctttttccg aaatttgaga agcaattttc gtaaattcct
    11041 tcttattact tgaagccaat catataatac agaaacagag aactatccaa aataaatcct
    11101 agaaaaagaa gtacgagctt cgctgatttt tgttaattta gaataatact aataggaatc
    11161 gtaaaaacag aaaaaaagca taatgataaa gggtcgggca gattttaatt gattcatctg
    11221 gtactctttt ttattgtcct tacattcata aagtttgaat gcaatgactt tattacatct
    11281 atatttatat ctaacagtag cgttgatgtg ttaattataa gttctatcac tttatgttca
    11341 attagtttgt gctcttatat gtgtgattga aaatatagat aaaaaaacaa tcatcggctt
    11401 tgacttcgat gattgttttc tttattttgt aaaaatccta tcacttgatt ttatggtaac
    11461 cttttatttt tgtcttcaaa cggcttggta agagagcagt aagcagtgca aatgggatcg
    11521 aaaagacata ccagaaaatc gtacgtccga taccataatg ttgatagagc tttgtcaaca
    11581 tcacgtgtaa caagatcaac cctaaaagca aggagccagc tataaaccaa tgattatccc
    11641 aatgcatgac ttcactaagt gccatcactc caaaaggata gaaataaaaa tagatcccga
    11701 ctaaggtcat ccgccagttc tttgtaaatg ttccgatatt caataaaaag aaaatcgctg
    11761 aaagcaaaat accgataggc ggaaaaagcc caaccaaaaa ggaatacaac atcccccaca
    11821 agatcatcac aggacctttg atcaatgcaa cgattgcgat gaacaaagtc agcaatatta
    11881 gtttttgtga taacgttgca acagtcatta atccaaataa aattaatgtg aaccaatgcc
    11941 aatgggactg ctgttgttct ttacgggaaa aatcgacccg gttggataaa taatctgcta
    12001 atttcggata agaacgctta aaatccataa actgttcctc cttcgtggaa aaggtaggtt
    12061 tagtatacga aaaataaaaa agaaatacat ccgtcttaag agggagtttt tcactgaaag
    12121 tggagaaaaa agaatggtaa attaaaatat tctcagttat actgaaaatt ataccaaatt
    12181 tcaaagaaga aagaggatgg gaatgaaagg caaactaaaa cgagaaatca atctgtttgg
    12241 cgcattagct accgttatgg gaaccgtgat cggtgcaggc gtcttcttca aaactgctgc
    12301 tgttactgct agtactcagt ctacaagttt gacattgcta gcttggcttt tgggtggttt
    12361 cttaacgatc tgtgctggat tgacagtggc tgaattagct acagcaatcc cggaaactgg
    12421 tggagctgta aaatatattg aagctgcgta tggaaaattg cccagctttt tattaggatg
    12481 ggcgcaaagc ttgatttact ttcctgctaa tattgctgca ttatcgatta tctttgcgac
    12541 acaattgacg aacttattac aattatctac cgattatctt ttgttgattg cagtgatcac
    12601 cgctgtttct gtgacagggt tgaatttgtt agggacaaaa gtcggtgctt ctgtacaatc
    12661 agtgacgttg attgttaaat taataccaat tgctgtgatt gtcatatggg gattattgac
    12721 cccaggacaa ggaactgtcc agttatttcc aatcgaagct ggaaaagatg ttacttttgt
    12781 cgaaggattg agcagtgcgt tgttagcaac gttgtttgct tatgacggct ggttaggtgt
    12841 cggtgcaatg gcaggggaaa tgaaacggcc ggaaaaagat ttgcctaaag caattattct
    12901 tggtttgagt tttgtaacag tcgtttatct attgatcaat tttgtttttt tgaaaacttt
    12961 gccgattgat catcttgccg gtaatttgaa tgcagcttct gaagcatcag acgtcatttt
    13021 tggaggaatc ggtggaaagc tggtcactat tgggatcttg atctcggtat acggtgcatt
    13081 gaacggttat acactaactg ggatccgcgt gccttatgcg atggctttag aagacgagct
    13141 acctttcagt aaacagctga caaagctttc aaagaagttt acggttcctt atgttcctgc
    13201 ggttttccag ttagcagtag cttgtattat gatgagcctt ggttctttcg actttttaac
    13261 agacatgttg atttttgtga tgtggctatt cagtttactg atttttatcg gtgtctttat
    13321 tttaagaaaa aaagcgccgg aattgccacg tccatataaa gtaccacttt atccaatcgt
    13381 tccgattatt gcgattctcg gtgctatttt cattttaggt atgacgatga tgacgcaaac
    13441 gaaattagca ttgatcggta taggtgttac cttgataggt atcccagtct attatcaaaa
    13501 aaagaaaaaa cgctcagaag agtaaaatat ttcaacaaca gacagaaaaa gtacgaacta
    13561 ggtcctctag atcttcgtgc tttttctatt tcaataaaac aagttcttct attttgattt
    13621 ttgctacttc tgcaagtagt gcttatgtgc taaagtaaga atgaaaagga gagataaaca
    13681 tgatatttga ttctcatacc catttaaatg ctgaacagtt caatgaagat attccagaaa
    13741 cgattcagcg agcacaagaa atgggggtaa cgaaaatggc ggtcgtgggc ttcgatacgc
    13801 caacgattga aaagtctttg caattaagcc atgactatcc aaatatatac agtattatcg
    13861 gctggcatcc gacagaggct ggcagttata cgaaagaaat cgaaaataaa ttacaagaac
    13921 aattgacttt accaaaggtt gtagcattag gggaaattgg cttggattat cattggatgg
    13981 aagacccgaa agaagtccaa gataaagtat ttcgcagaca gattgctatc gcaaaagaaa
    14041 tgaacctacc aatcagtatt catactagag aagcacttga agacacgtat caaatcctaa
    14101 aagaagaaga tgttcgagat atcggtggaa tcatgcatag tttcagtggt gattatgaat
    14161 gggcaaaacg atttcttgac ttagggatgc atatttcatt tagcggtgtc gtcactttca
    14221 aaaaagcatt agacgtccaa gaggctgcag caaatgtacc aatcgaacga ttacttgtgg
    14281 aaacagatgc cccttatctg gcaccagttc cttttagagg aaagcgaaat gaaccaggct
    14341 atactcgtta tacagtagaa aagattgctg aacttcggaa tcttccgaca gaagaagtgg
    14401 ctgcccaaac atggacaaat gcgcaccgat tatttggtct aagcgaacat ggataagatg
    14461 aagatccaag agattatagt cgttgaagga aaagacgata caagaaggct gcgggaagta
    14521 gttgatgcag atacgataga aacaataggg tctgcaatca atgaagagat cctgatgcaa
    14581 attgaacatg cccaagagac tcgtggagtg atcgttttta ccgatcctga tttttcagga
    14641 gaaaaaataa gaaaaatcat tatggaagct gttcctcagg caaaacatgc ttttttgccg
    14701 cgcacacaag ctaaaccaaa gaaaaaggga agccttggtg tcgagcatgc aagtgatgct
    14761 gctattttag aagcattgaa aaaggtcgtg acgccagaca tttcccaaac ggctgtttct
    14821 gaaatcacta gacaagacct tttagactat ggtttaatag ctggggcaca agcaaaagaa
    14881 aaacgagaaa aactaggtga cgagttgcgc atcggttaca cgaacggcaa acaattggaa
    14941 aaacgtttga agatgtttcg tattacgccg aaagaactcg cccaagcaat gaaaatagtg
    15001 gaggaacaac atgagtgaat atcgtgaaat tgcgacaccc tctagaacaa aagagatttt
    15061 attgaaacat ggtttttcat ttaaaaaaag tttaggacaa aattttctga ctgaaccaaa
    15121 tattttaagg aaaatcgtag aaactgcgca aatagatgaa catacgaacg tgatcgaagt
    15181 tggaccagga atcggtgcat tgacggaaca attagccaag catgcaaaac aagtcgtagc
    15241 atttgagatc gatgatcgtt tgatccctgt attggctgat acgatgcaac cctatgacaa
    15301 tgtgactatt gtacatcaag atattttaaa gaccgatttg tcaacagctg ttagagaaac
    15361 atttcatgag gagcttccgt taaaagtcgt agcgaattta ccttactata ttacgacacc
    15421 gatcatgatg cattttcttg aatcagattt ggttgtggat gaactagtcg tcatgatgca
    15481 aaaagaagta gctgatcgta tctctgctga accaggaacg aaagcttatg gttcattatc
    15541 gattgctgtt caatattata tggaagccag tttagcattc atcgtaccaa aaacagtatt
    15601 tgtccctcag ccaaatgtag attcagcgat tttgaaactg acaagaagag atataccggc
    15661 agtcgaagtg acagacgaaa aagcattttt ccgattgaca aaagcggcat ttcagcaaag
    15721 aagaaaaacg ctttggaata acttgcagca ttcgtatggc aaagatgacc agacaaaagc
    15781 atggttagca aaaagcttgg aaacagctgg aatcgatcca aaacgtcgtg gggagacttt
    15841 gtcattacaa gaattcgccg cattaagcaa tgcgatgtca gaaaatatgc agtaaaaaaa
    15901 gaggttgtga cagaagttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    15961 aaaaatagtt cctcatgttt ttcgtttgtt tgggcttgtt accgcagatg caagcttttg
    16021 aacaccgttt aagaaagagg ttgtgggcct gccgtttaac tctaagtcac aaggaacaat
    16081 ttcaaaaaac agcttctcat gtttttgagt tagttggtct tattacttga tgcaagcttt
    16141 tgaccactat tatgccgaac aaggagatgt gacacaaatt ttgtcacatc tcctattttt
    16201 gtttcttgat caaaaaattc ttatacttga tactcacact cattttgtat ggatttagaa
    16261 aagaatatgt gtcttgacag ttattagtaa gtgagttata ctaagtcgac atttatgtgt
    16321 caagacatat taggagagag aaaaaatgag aaaaaattca gttcgaaaac taacgatttc
    16381 tgctttattg attgggatgg gggtcattat cccaatggtc atgcctaaaa tcatgattcc
    16441 accagcttcc tttactttgg cgtctcatgt gccattattt attgcgatgt tttttacacc
    16501 aggagttgca gtggctgttg cgctaggtac gacatttggt ttctttttaa caacgccggt
    16561 tatcatcgca ttgcgagcat tgtcacatct tgtgtttgcg ttgattgggg cttggtactt
    16621 gcaaaagcat ccgagtatcg ttttaaaaaa tggacagttt acttttgcta attggcgttt
    16681 ccaagggttc aattttgtga tcggtgtgat tcattcttta gcagaaatgc tagtagtcag
    16741 catcttttac tttattggta atatgcctga aacatattat tctcaaggat atttttatac
    16801 agtattcatc ttgatgggga ttggtgggct tatccacagt ttagttgatt ataatattgc
    16861 ttattttata gcaggaacac tcagcaaata ttttgatatc cccgtattta cggcagcaaa
    16921 aaaagaaaat cggaaagagt cgattcaagg gtacgcgaag aaaacgacat attagacatg
    16981 atctctcaaa aaaaaaactc tcatctagta atggataata aatcctctaa gatgagagtt
    17041 ttttgtccta aaaagagctg caccaacatc ttctggcaca gctctaacag ggggcaattt
    17101 atggaagttt gatgacttgt tctgtttctt tatcaaagaa gtggcctttg ctgatgttga
    17161 aggcaaggtc gactatttcg cctggtttat ggaaatctct tgcatctact tttgagatga
    17221 actcagtaga accagttttt gtgtaaagca tcgtttcagc tccaagtaat tcggaaacga
    17281 caacttctga tttcactacg gcttcaggtg aagcttcaag ggctacttgt tcactgtgga
    17341 tgtcttctgg acgaataccg aagataactt cttttccttc ataacctttt tcaactaata
    17401 ctttgttttt tccttcagga atacgaagct taagcccatg tccatctgtg ataacaccat
    17461 ttttcaatgt cacattaaag aagttcatgg caggagatcc aataaagccg gcaacgaaca
    17521 tattgacggg tgtatcataa acttctttcg gtgtaccgat ttgttggata aatccgtctt
    17581 tcatgataac gattcggtca gccatagtca ttgcttctgt ctgatcgtga gtaacataga
    17641 tggtcgttgt ttcaagacgc tggtgaagtt tggcaatctc agcacgcatg gctacacgta
    17701 atttagcatc taaatttgat aatggctcat ccattaagaa gacttttgca tcccgaacga
    17761 ttgcccgtcc caatgccaca cgttggcgtt gtccaccaga taaagcagct ggttttcgtt
    17821 tcaagtattc tgtcagacct aagatctctg ctgcattgtc cacacgtttt ttgatgtctg
    17881 ctttatcata tttacgtaat ttcaagccaa aagccatatt atcaaaaaca gtcatatgag
    17941 gatacaatgc gtagttttgg aaaaccatgg caatatcacg gtctttaggt gccacgtcat
    18001 tcatgacttt tccgccgata tttagttctc cttcagagat atcttccagt cccgcgatca
    18061 ttcgcaaagt agttgacttt ccacaaccgg aaggaccaac aaaaacgata aattcacggt
    18121 ctttgattga tagattaaaa tcagttacag aataattttc tgcattgtca tattttttat
    18181 atacgttttt cagagccatt tctaccataa tacgttccac actttctttt ttagttttat
    18241 taactattta aagtataaat gaaagcgtta actaacttca atgtcatgtt gaacaagaat
    18301 tttttctttt ttttgtcaat gtgctagttt taagctttaa taaatatcca taagaaataa
    18361 aaactgatac actggagtaa ataagtatgt taacaatatc aaaaggagtg tctcttatga
    18421 aaatcgcttt gatcgctcat gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt
    18481 ataaaacaat attggaaaaa catgaacttt ttgctaccgg gacgactggg cagaggatca
    18541 cggaagcaac cggcttgccg atccatcggt ttaaatccgg accgttaggc ggggatcagc
    18601 aaattggagc catgatttcg gaggatcagt tagatttggt gatttttctt cgagacccgc
    18661 tggctgcaca gccccatgaa ccagatgtaa cagcacttat ccgtttatct gatgtctatg
    18721 agattccgtt agctacgaat atcggaacag ctgaggtgct actaagagga ctggaagccg
    18781 gcttcctaga ttttcgaaga atcgtccatg aaatagacaa gcatccactt tcattttgat
    18841 ggataagcgt atggaaaaat aacgtttaat gttcgcctat atgtagctat aaaagctaaa
    18901 caaactgata atatgcgata taatacgaac gtattggata atataaacaa taatattagt
    18961 ttttcgtatt tgcagtaaat gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa
    19021 aataatacag agtagaaaat aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg
    19081 acgaaggacg taatgttcgc ggttttattt tcgcattcgc tgcatgatac gatgtagcag
    19141 ctcttaaagg agatgctaaa aatgaagaaa caccggttag acgcacgtat gattgctatt
    19201 atgggcttat tgattgcatt gatggtcact ttgtcccgac tggtagcaat cgagacccca
    19261 tttattaaaa tcagtgtcac atttattcca caagtgatca tgggcattct ttttggtcct
    19321 ttttggtcag ggataggcgc agttttagct gatttagtag gaatggccct cttctcgaaa
    19381 tctgcattct ttattggatt tacattgaat gcatttattg aaggagcaat ctacggattc
    19441 tttttctatc gaaaagagat cacttggaaa aatgccattc tggctacgct tagtgtcacg
    19501 ctgatcatca acttattttt gacaccatta tggcttgcgt tgatgtacca tgtgccattg
    19561 ttcagctggg tcgtttgggc gccccgcctg ttaaaaacag taatatggtt accgattcaa
    19621 tcaattgcca tttattacgt aggacgtagt attccttaca aaaaaatttt aagaagttta
    19681 gcgatccacg ctaaataaaa aacaagcagt cttcccatcc cagaagactg cttgttttta
    19741 ttgtgctgtg aattgtgctt gatagagccg tcggtaataa ccatcttttt gaagcagctc
    19801 atcatgggtg cccatttcga caatagctcc ctgatccatc actaatatct ggtccgcatt
    19861 tttgattgtg gacaggcgat gggcaatcac aaaactggtt tttccttcca tcattgctaa
    19921 gaaggcttgt tggatttttt tctctgtcaa tgtatcgact gagctggtag cttcatctaa
    19981 gatcaacata gggggattac tgatcatcgt tcgagcaatc gtcaacagtt ggcgttgtcc
    20041 atctgatatc tttaaccctt gaccgccaat cttagtatct aatttttccg gcaaacgttc
    20101 tacaaattca tacatataag aggcttttag ggcatcattg atttctgcgt cacttgcttc
    20161 cggattccca aaacgaagat tttctcgcaa tgtactgtca aatagccaag tatcttgtaa
    20221 caccattcca aaacttttac gtaatgtatc acgttggata gagcggatgt catggtcatc
    20281 gactgtaatt gttccttttg ttgtgtcata aaaacgcatc agcaaattaa caagtgtgga
    20341 ttttccggcc cctgtttttc ctactatagc aatggtttga cctggataag cagtaaaatt
    20401 gaaatcttcg atcaaacgct gcttggggtc ataagaaaaa gaaacatgat caaaagagac
    20461 ttttcctttg atatttttgg gttccaaggc ataggcatct ggcaaatcag gcttttcttc
    20521 tggctgatcg atcaaatcaa atgctcgttc tagaccagaa aaagcggttt gcagttgtgt
    20581 cgtgattcca gaaagttcga taaatggttt agaaaactga cttgagtaga tcgtaaagct
    20641 ggaaatcaca ccaacagtga ttgacgaacc actcttcaat gccaataggc cgccaactaa
    20701 accaatcgat aaataagcaa ggtgatctac aaaacgagaa gacgggtttg tcaaggaaga
    20761 agaaaattgt gcccgttgtc ctttttcata tagatcagca tttagcagtt caaattgctg
    20821 ttgactagct tcttcttgct gaaaagcttt cacgattttc tggttaccga ttcgttcggt
    20881 cacaaagccc gagatatccc caataatcct ttgctgtcta tcgaaatttg tttgtgaagc
    20941 acgagcaacg atccaactca caaggaaaat aattggtgta gaacaaagaa cgaccaatgt
    21001 cagaagtgga cttagacgca tcataaaaat aaacgaaaga atgatgattg ccactccaga
    21061 aaataattga ttgaaggccg cagaaattgc aatggaaata ttgtccatat catttgtaaa
    21121 tcgactgacg atatttccgt gggagttttg atcataataa cgaagtggca atcgattcag
    21181 atgagcaaat gcatcttttc ttaattttgc aacggataag taagctaacc gattgcttaa
    21241 cacttggatg agccattgac tgactacgtt gattaaaacg ataccaccga atagaataag
    21301 taaatggaac agtcgattga agcggacctg tccttctcca atcatttgat cgatactttg
    21361 accaatttgt aacgtcatca caactgttgc aatcccattg atgattccta gtatcacagc
    21421 tagccaaatg tcttttgcat aggcagtaag gtaaggaatg aatcggcgga gcgtctgcaa
    21481 ggataatttt tttactttca taactattct tcctcctgtg atgcgacgat ttcctgatat
    21541 tctgctgatt gagcaagtaa ttcttcatgg gtaccctgag cagcaatctt accttgatcc
    21601 atcactaaga tatgatgtgc ttcttgaata gagcggacac gttgggagat aataatcact
    21661 gtactgccaa gataagtctg tagcgcctta cgcaaatcca agtctgtttg ataatccaac
    21721 gcacttaaag agtcatctaa aatcagtaat gctggcttgg caatcaaagc tcttgcaatc
    21781 gtcagtcgct gtttttgtcc gccagagaaa tttttcccat tttccatgac ttttgtatct
    21841 aaaccatttg gaagctgacg gacaaattct tctgcctgag cgattttcag agcttcccaa
    21901 cattcttttt ctgtggcgtc tttcttaccc cattgaaggt tttcacgaat agtcccggta
    21961 aatagtacag aagtttgggg aacaagagca atttgctgac ggagatgatc cagctgccaa
    22021 gaacgaacat ccattccgtc cattataact gtaccggaac tgacatcata gaaacgcgga
    22081 atcaattgtg tcaaagtcgt tttcccgctt ccggttggac caatgattcc aaggactgta
    22141 tttcgcttca gctgaaatgt aatattttgg agagaaagac cagaatcagg agtataacgg
    22201 aagtcaacat ggtcaaaagc gataattgct tcagaagaaa aatcgggagt agtactgacc
    22261 tcagcttcaa gaatcgaatt ttctgtgtct agcacctctt tcacccgatt tcctgaagct
    22321 tcggcacgtg tgaagatcac gacgaggttt gaaacaacga tcagtgccaa aagcatttga
    22381 ttcatataat tgatcaaagc aagaacttgt ccttgttgca agctaccaat atttactttg
    22441 atcccgccaa ctgtcagcaa acagatgatc ccgacattca tgatcaaggt tgtagcaggt
    22501 gataataggg cagagatatt ggaaacacgt aaataattgt tcgctagatc atttgtgctt
    22561 ttatcgaact tctctgtttc tttttttgtc tgcgcaaatg cgcggatgac acgaacaccg
    22621 ctaagattct gactaacgtg ttcattgagc tgatctagtt tttcttgcac tttttggtag
    22681 agaggaacgg tttttttgat gataaaaaat aaaatgattg agaaaatagg caataatgct
    22741 aagaaaaaca gacctacttc ccagtcgatc acaaaagcca tgaccactga cccgatactt
    22801 aaaaacggtg ctctgatcaa taaacgaatg accattgcta aagctagctg aagctgattg
    22861 atgtcgtttg tcattcgggt gatcaaggta tctgttccaa aatgattcaa ctcgttgtga
    22921 gagaaagtat tgattttttt gattagctga ttacgtaact ctgtaccaaa tccttgtgaa
    22981 gctactgaag catagtattg gcaaatgata gcacaaatca gaccaatcac agacatagcg
    23041 accatccata agcccatttc aatgattttt cctcggtcat tcatttttag tccattatcg
    23101 atcaaggaag ccatcattaa aggcaggaca agttcgaaga cagcttcaag aaatttgaag
    23161 aacgggccca aaatgatttg cttacgatag tcttttgcat aacgtaataa ctgaatcata
    23221 tgattcctcc tattatttat ttgtccacaa ttgaaaaaac aagtactacc acattgtagc
    23281 ttaggaaatt tagaatgaaa agagaacaag ccttttcata aatgaaagtt ttattatgaa
    23341 gaagctttag aaattgaata gactattttt tttaaaaaaa agaaaatgag aaaacatgcc
    23401 agtctcttca ttcataggaa cg
//
LOCUS       DAYYDS010000041        23261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000041 DAYYDS010000000
VERSION     DAYYDS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.041"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHA02_001564"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHA02_001564"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8669894.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACHA02_001565"
     CDS             complement(1172..1867)
                     /locus_tag="ACHA02_001565"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8669895.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACHA02_001566"
     CDS             complement(1880..2596)
                     /locus_tag="ACHA02_001566"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8669896.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACHA02_001567"
     CDS             2926..3534
                     /locus_tag="ACHA02_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8669897.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACHA02_001568"
     CDS             3859..4182
                     /locus_tag="ACHA02_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8669898.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACHA02_001569"
     CDS             4364..5734
                     /locus_tag="ACHA02_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8669899.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACHA02_001570"
     CDS             complement(5864..7165)
                     /locus_tag="ACHA02_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8669900.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACHA02_001571"
     CDS             complement(7519..9510)
                     /locus_tag="ACHA02_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8669901.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACHA02_001572"
     CDS             complement(9654..9920)
                     /locus_tag="ACHA02_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8669902.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHA02_001573"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHA02_001573"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8669903.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHA02_001574"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHA02_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8669904.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACHA02_001575"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACHA02_001575"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8669905.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACHA02_001576"
     CDS             complement(14080..14601)
                     /locus_tag="ACHA02_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8669906.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACHA02_001577"
     CDS             complement(14604..15446)
                     /locus_tag="ACHA02_001577"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8669907.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACHA02_001578"
     CDS             complement(15439..16164)
                     /locus_tag="ACHA02_001578"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8669908.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACHA02_001579"
     CDS             16340..16636
                     /locus_tag="ACHA02_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669909.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACHA02_001580"
     CDS             17204..18796
                     /locus_tag="ACHA02_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8669910.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACHA02_001581"
     CDS             18809..19549
                     /locus_tag="ACHA02_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8669911.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ACHA02_001582"
     CDS             19717..21066
                     /locus_tag="ACHA02_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8669912.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ACHA02_001583"
     CDS             complement(21194..21784)
                     /locus_tag="ACHA02_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8669913.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22020..23099
                     /locus_tag="ACHA02_001584"
     CDS             22020..23099
                     /locus_tag="ACHA02_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8669914.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         6661 a         4820 c         4527 g         7253 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaggcggat tttataatta acttagccat ttggaaataa aaaaacgcca
    21961 cgtgaattcc atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca
    22021 tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc tactcagaac
    22081 gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc
    22141 tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt actcagctta
    22201 accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac gacgccgatg
    22261 ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc ttatgggccg
    22321 acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa tggtccccgg
    22381 acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt atccctggga
    22441 ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat atggacctgc
    22501 tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg
    22561 tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa accttcggcc
    22621 attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt
    22681 tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag gatgccgaat
    22741 cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc ggccagctgt
    22801 tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat gccttgcagg
    22861 atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg acgagcgaaa
    22921 accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg
    22981 aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag attttgggct
    23041 atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg acggcttgac
    23101 acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa gctggccggg
    23161 gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac tcgattagaa
    23221 aaattagacg gttggtggta acgagcaacc gggtcaccat g
//
LOCUS       DAYYDS010000042        22031 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000042 DAYYDS010000000
VERSION     DAYYDS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.042"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            367..555
                     /locus_tag="ACHA02_001585"
     CDS             367..555
                     /locus_tag="ACHA02_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669915.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="ACHA02_001586"
     CDS             545..805
                     /locus_tag="ACHA02_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFM8669916.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="ACHA02_001587"
     CDS             846..1073
                     /locus_tag="ACHA02_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669917.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="ACHA02_001588"
     CDS             1138..1710
                     /locus_tag="ACHA02_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669918.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="ACHA02_001589"
     CDS             1711..2022
                     /locus_tag="ACHA02_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8669919.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="ACHA02_001590"
     CDS             2013..2273
                     /locus_tag="ACHA02_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669920.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="ACHA02_001591"
     CDS             2434..2793
                     /locus_tag="ACHA02_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669921.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="ACHA02_001592"
     CDS             2790..4433
                     /locus_tag="ACHA02_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8669922.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="ACHA02_001593"
     CDS             4468..5550
                     /locus_tag="ACHA02_001593"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFM8669923.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="ACHA02_001594"
     CDS             5594..6766
                     /locus_tag="ACHA02_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8669924.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="ACHA02_001595"
     CDS             6753..7457
                     /locus_tag="ACHA02_001595"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8669925.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="ACHA02_001596"
     CDS             7462..8622
                     /locus_tag="ACHA02_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8669926.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="ACHA02_001597"
     CDS             8698..8976
                     /locus_tag="ACHA02_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFM8669927.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="ACHA02_001598"
     CDS             8957..9328
                     /locus_tag="ACHA02_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFM8669928.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="ACHA02_001599"
     CDS             9344..9748
                     /locus_tag="ACHA02_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669929.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="ACHA02_001600"
     CDS             9745..10155
                     /locus_tag="ACHA02_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669930.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="ACHA02_001601"
     CDS             10216..10803
                     /locus_tag="ACHA02_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8669931.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="ACHA02_001602"
     CDS             10876..11343
                     /locus_tag="ACHA02_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFM8669932.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="ACHA02_001603"
     CDS             11389..11697
                     /locus_tag="ACHA02_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669933.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="ACHA02_001604"
     CDS             11892..18626
                     /locus_tag="ACHA02_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8669934.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18657..19502
                     /locus_tag="ACHA02_001605"
     CDS             18657..19502
                     /locus_tag="ACHA02_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM8669935.1"
                     /translation="MNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSFREIGESIEL
                     SEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSLKSLSKTEID
                     SQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYTKNVWEKKGV
                     FKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFINMKDTQILE
                     VSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGHANVEIELYE
                     ERDLF"
     gene            19499..20548
                     /locus_tag="ACHA02_001606"
     CDS             19499..20548
                     /locus_tag="ACHA02_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669936.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="ACHA02_001607"
     CDS             20552..21376
                     /locus_tag="ACHA02_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669937.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="ACHA02_001608"
     CDS             21394..>22031
                     /locus_tag="ACHA02_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8669938.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DAYYDS010000043        21988 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000043 DAYYDS010000000
VERSION     DAYYDS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.043"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACHA02_001609"
     CDS             <1..395
                     /locus_tag="ACHA02_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8669939.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ACHA02_001610"
     CDS             398..1147
                     /locus_tag="ACHA02_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8669940.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ACHA02_001611"
     CDS             1159..1428
                     /locus_tag="ACHA02_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669941.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ACHA02_001612"
     CDS             1433..1597
                     /locus_tag="ACHA02_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669942.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ACHA02_001613"
     CDS             1590..1892
                     /locus_tag="ACHA02_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669943.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ACHA02_001614"
     CDS             1889..2050
                     /locus_tag="ACHA02_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8669944.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ACHA02_001615"
     CDS             2047..2352
                     /locus_tag="ACHA02_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669945.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ACHA02_001616"
     CDS             2352..2708
                     /locus_tag="ACHA02_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM8669946.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="ACHA02_001617"
     CDS             2668..2913
                     /locus_tag="ACHA02_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669947.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ACHA02_001618"
     CDS             2910..3329
                     /locus_tag="ACHA02_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8669948.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ACHA02_001619"
     CDS             3326..3883
                     /locus_tag="ACHA02_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM8669949.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ACHA02_001620"
     CDS             3880..4176
                     /locus_tag="ACHA02_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669950.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ACHA02_001621"
     CDS             4253..4666
                     /locus_tag="ACHA02_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8669951.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ACHA02_001622"
     CDS             5012..5386
                     /locus_tag="ACHA02_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669952.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ACHA02_001623"
     CDS             5423..5590
                     /locus_tag="ACHA02_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669953.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ACHA02_001624"
     CDS             5616..5960
                     /locus_tag="ACHA02_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8669954.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ACHA02_001625"
     CDS             5965..6246
                     /locus_tag="ACHA02_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669955.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ACHA02_001626"
     CDS             6349..6663
                     /locus_tag="ACHA02_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8669956.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ACHA02_001627"
     CDS             6641..8335
                     /locus_tag="ACHA02_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8669957.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ACHA02_001628"
     CDS             8355..9533
                     /locus_tag="ACHA02_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8669958.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ACHA02_001629"
     CDS             9496..10182
                     /locus_tag="ACHA02_001629"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8669959.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ACHA02_001630"
     CDS             10182..11342
                     /locus_tag="ACHA02_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8669960.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ACHA02_001631"
     CDS             11352..12227
                     /locus_tag="ACHA02_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669961.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ACHA02_001632"
     CDS             12224..12535
                     /locus_tag="ACHA02_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669962.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ACHA02_001633"
     CDS             12525..12878
                     /locus_tag="ACHA02_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8669963.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ACHA02_001634"
     CDS             12868..13269
                     /locus_tag="ACHA02_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669964.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ACHA02_001635"
     CDS             13262..13666
                     /locus_tag="ACHA02_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669965.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ACHA02_001636"
     CDS             13678..14286
                     /locus_tag="ACHA02_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8669966.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ACHA02_001637"
     CDS             14306..14668
                     /locus_tag="ACHA02_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669967.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ACHA02_001638"
     CDS             14671..14853
                     /locus_tag="ACHA02_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669968.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ACHA02_001639"
     CDS             14870..18289
                     /locus_tag="ACHA02_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8669969.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ACHA02_001640"
     CDS             18340..19077
                     /locus_tag="ACHA02_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8669970.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ACHA02_001641"
     CDS             19087..21378
                     /locus_tag="ACHA02_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8669971.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ACHA02_001642"
     CDS             21402..>21988
                     /locus_tag="ACHA02_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8669972.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAYYDS010000044        21922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000044 DAYYDS010000000
VERSION     DAYYDS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.044"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1224
                     /locus_tag="ACHA02_001643"
     CDS             85..1224
                     /locus_tag="ACHA02_001643"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8669973.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1217..2017
                     /locus_tag="ACHA02_001644"
     CDS             1217..2017
                     /locus_tag="ACHA02_001644"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8669974.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            2017..2844
                     /locus_tag="ACHA02_001645"
     CDS             2017..2844
                     /locus_tag="ACHA02_001645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8669975.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2849..3556
                     /locus_tag="ACHA02_001646"
     CDS             2849..3556
                     /locus_tag="ACHA02_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8669976.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            3656..4522
                     /gene="rfbA"
                     /locus_tag="ACHA02_001647"
     CDS             3656..4522
                     /gene="rfbA"
                     /locus_tag="ACHA02_001647"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8669977.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4536..5108
                     /gene="rfbC"
                     /locus_tag="ACHA02_001648"
     CDS             4536..5108
                     /gene="rfbC"
                     /locus_tag="ACHA02_001648"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8669978.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5130..6158
                     /gene="rfbB"
                     /locus_tag="ACHA02_001649"
     CDS             5130..6158
                     /gene="rfbB"
                     /locus_tag="ACHA02_001649"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8669979.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6256..7107
                     /gene="rfbD"
                     /locus_tag="ACHA02_001650"
     CDS             6256..7107
                     /gene="rfbD"
                     /locus_tag="ACHA02_001650"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8669980.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7142..9175
                     /locus_tag="ACHA02_001651"
     CDS             7142..9175
                     /locus_tag="ACHA02_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8669981.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9219..10499
                     /locus_tag="ACHA02_001652"
     CDS             9219..10499
                     /locus_tag="ACHA02_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8669982.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10709..11515
                     /locus_tag="ACHA02_001653"
     CDS             10709..11515
                     /locus_tag="ACHA02_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8669983.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11527..12747
                     /locus_tag="ACHA02_001654"
     CDS             11527..12747
                     /locus_tag="ACHA02_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8669984.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12737..14323
                     /locus_tag="ACHA02_001655"
     CDS             12737..14323
                     /locus_tag="ACHA02_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8669985.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14362..16500
                     /locus_tag="ACHA02_001656"
     CDS             14362..16500
                     /locus_tag="ACHA02_001656"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8669986.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(16868..17851)
                     /locus_tag="ACHA02_001657"
     CDS             complement(16868..17851)
                     /locus_tag="ACHA02_001657"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8669987.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            18229..19620
                     /locus_tag="ACHA02_001658"
     CDS             18229..19620
                     /locus_tag="ACHA02_001658"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8669988.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            19636..20676
                     /locus_tag="ACHA02_001659"
     CDS             19636..20676
                     /locus_tag="ACHA02_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8669989.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            20695..21780
                     /locus_tag="ACHA02_001660"
     CDS             20695..21780
                     /locus_tag="ACHA02_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8669990.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            21813..>21922
                     /locus_tag="ACHA02_001661"
     CDS             21813..>21922
                     /locus_tag="ACHA02_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFM8669991.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKT"
BASE COUNT         7097 a         3572 c         4488 g         6765 t
ORIGIN      
        1 aaaaagtaga aacaagagtt cttattgtaa atttatatca gtaatttata ataattatga
       61 ctcttaatta aagtaggtgc ccatatgctt gtttctttta gtatggttat aagatttttt
      121 ctgacaattt tattatcact gatattaaca cctgtattca aaatcatttc agtacaaaca
      181 ggtatggtcg acaaaccaaa tgaaagaaga ataaataaag ttccaatgcc ttcagcgggc
      241 ggactgccta tctttatttc atttgttata tcgactttat tcttattccg taaaattatc
      301 cctcaatcgt atattgtacc gatcttgatt gcttctttag taatcatagt cacaggagtg
      361 ctcgatgata aatatgagct gacacctaaa caaaaaagta tagggatctt gattgcttct
      421 cttattattt attttgtagc agatatacgt attgattcct tcacgcttcc ttttattgga
      481 tatatccaac taggctggct tagttttccc gtaacgattt tttggatctt cggtatcacg
      541 aatgcggtca atctgattga cggtttggat gggctggcgg cagggatttc attgatcggt
      601 ttaattacga taggaatcat tggatatttt ttcctccatg cttctacagt gtatattcca
      661 gttgttattt tctgtcttgt agcaagtatt atcggttttt ttccttacaa tttttatccg
      721 gcaaagattt atttaggcga tacaggagct ctattcctag ggtttatgat ggccgtcctt
      781 tcactacaag ggctgaagaa cgtgactttt gtttcatcga tttctttgct gatcgtcatg
      841 ggagtgccgg taacagacac gttttttgct atcattagga gaaaagcaaa ccgtgtttct
      901 ttttctacag cagataaaaa acatcttcat caccgactaa tcagtttagg ctttactcat
      961 aaaggtgctg tcctgacgat ttatgcgatg gctttgatgt tttctttcac agctatggtc
     1021 atgaattata ctggaagaat cggaacgatc gtattgatta ttgccatgtt gtttgctgca
     1081 attttattat ttgaattgat tggtctgatc aatgagaaac atcagtttat tttaaaaaca
     1141 ttgcgatttt tagggaataa agagtaccgt acgcaagtaa aacagaaata tttgaagaaa
     1201 tggaaccgtc atatagatga ataacaaatt aaaccagccg atccttatta gtattgtgac
     1261 ggcaaacagt gataaaattt ttcggacact agataatttt atttcaactg taaaagatga
     1321 aacagcagta aaaatccgaa tttttgacaa caattcactt cccgagtaca aaaaaagact
     1381 aacagagtat aggaaatatc cctacatcga tatctatttt catgatgaaa attccggatt
     1441 tggttatgga cataatcaca atctgatagc tagcgattct cgctatggca ttatttgtaa
     1501 cccagatatt ttagtggatg aagagacagt tacagcattg gtcttgttat tgaaagaaca
     1561 tccggaatgt gcgatgttag cacctaagat tcttaatgaa gatggaacaa ctcagcacct
     1621 catccgtgag cagctggctg tttttgatta cgttttgcgc tttgtgcctt tcaaatgggt
     1681 caaaaaaaag tttgacaaac gactggctgt gtttgaatgc cgaggattgc ctactgatgc
     1741 taatagttat gtacggatga tctcaggtag ttttatggta gctgatattg ataaatttaa
     1801 agagataaag gggtttgatg agcgtttctt catgtacttt gaagacaacg acttgtgttt
     1861 gagttttgaa aaagcaggtt acaagttatt gtacacaccc ttttatgcag ttatccattt
     1921 ttacgggaag ggtgcgcatc gaaatgcaaa actgtttcgc gtgtttttga cttctatgat
     1981 gaaatttttc aataagtggg ggtggcgttt tttctgatga aaccagaaat attagtagtc
     2041 gttgtgcttt atcagcaact attcagtcaa tctccagctt atggaccatt aagcaaagca
     2101 cttagcgaaa agcaaattca gcttattatt tacgataata gtcctcaaag gcagcaaaat
     2161 gagctgtttg aaaaagaaaa tatcgtttat catcatgatc cgcaaaatcc tggtttagcg
     2221 gtggcttata attttgcgtt gtctcaagca gatgaaggaa cacgttgtct cgtcactctg
     2281 gatcaggata cgaggctagt cgacgattat tttgaactat tgcgtaaagt aacctttaca
     2341 gatgaatgtg ttgctgcagt tccgatgatt ttttctggga gccgacaagt ttctccccta
     2401 tattcagatc gttatatcaa tcgtacagct caagcagtag aagttgaagg cccaacagct
     2461 gaacgaatca tggcaatcaa ttcaggtgtt gcctggtctg tcaaatttct aaaaaagatt
     2521 ggcgggttta atcctgcttt ttctttagat tttttggatc attggctgtt ttggaaagtg
     2581 gatcagttgg aaaaaactgt ggagatcctt cctgtacgaa tggaacatga tctttcggtt
     2641 ttagactatg aaaaagtttc aagtaatcga taccgttcta ttttacaagc agaaaatcga
     2701 ttttatcatg aatatgatca aaggcattta tccagtcacc gacgacaatt attgttaaga
     2761 acagctaaac agttcttgac cgtcaaaaac cggcatatct ggcggatgac tttgcgctca
     2821 tatgttaaga attggaaggt gtgaattcgt gcgttcggtt tgtatagcta cctttaatgg
     2881 aggcagatat ttaagagagc agttagatag tattttatct cagctttcag cagaagatga
     2941 agtagtcatt tctgatgatg ggtcagtaga tgatacatgg gatatattaa ctgaatacgc
     3001 agaaaaagat aagcgaatcc agcttttttc ggggcctcgt caaggattga tcgctaattt
     3061 tggttatgcg atccagcaga cgaaaggaga tctaatcttt ttggcagacc aagatgatgt
     3121 ttggttggag acaaaagtga aaaagatcac tgactatttt gagcagaatc cggatcagct
     3181 agtagtagtt tctgatttaa tcattgtgga cagcgagctg caagagatcc atgattcgta
     3241 tttttcgttt cgaaattcca aagaaggatg gcttaataat gtgataagaa gtcactatat
     3301 tggtgctggt atggctttcc ggtcttcttt aaaacccgat attttgccga tcccgtcaga
     3361 agtgccgatg catgatatgt ggattggttt gctgggagga aagaagactg ggttcctaag
     3421 agaaccgtta actctgtacc gaagacatga tttaaacgcg agtgagattg aaacacattc
     3481 taaattacgg caaaaaattt cttggcgtgt tcatctgatt acggcgttga tcaaacgaaa
     3541 atggtttaaa cgttgataaa agatgtttaa atccttaata tttccatgac aaatataaca
     3601 attgatgaga taataacaaa gtgaaatttg aaatcaaaaa ggagagcaga tatccatgaa
     3661 gggaatcatt cttgctgggg gcagcggaac acgtctgtac cctttaacaa aggcaacatc
     3721 gaaacaattg atgccgattt atgacaaacc aatgatttat tatccaatgt ctactctgat
     3781 gttggcggga atcaaagaaa ttctgattat ttcaacgcca caagatacac cacgtttcaa
     3841 ggaattattt ggaaacggaa atgatttagg actgcagatc gaatatgcag tacaagaaag
     3901 tccagatgga ttggcacaag cgtttatcat tggagaagaa tttattggag atgatagtgt
     3961 ctgcttggta ttaggggaca atatttacta tggtggcggt ctgtctaaaa tgttgcaacg
     4021 agcagcttca aaagatacag gcgcgacagt atttggctat catgtcaatg atccagaacg
     4081 ctttggtgta gtagaatttg atgagaatat gcaagctttg tcgattgaag aaaagcctgc
     4141 tcagccaaaa tcaaattacg ctgtaactgg cctttatttt tatgacaatg aggtaatttc
     4201 tatcgctaaa ggtatcaaac cttcagaacg tggagaacta gagattacgg atgtcaataa
     4261 agcatatttg gataaaggaa aactatccgt agaacttatg ggacgcggat ttgcttggct
     4321 tgatacagga acacatgaat cattgcttga agcatctacc tttatcgaaa caattgaaaa
     4381 acgtcagaac ttaaaagtgg cttgtttgga agaaattgct taccgtatgg gctacatcga
     4441 taaggaacaa ttgctggccc ttgcacagcc attgaaaaaa aatcaatacg gtcaatattt
     4501 attgaatttg gccgctgaat aaggagtcct tacagatgaa agtaacagat acaaaattaa
     4561 cagatgtcaa aatcgtagaa atggacgtct ttggcgatca tcgcggtttc ttcacagaaa
     4621 gttattcaaa agcaaaattt gcagaacatg gattgaaata tgactttgtt caagacaacc
     4681 attcactatc agctgaagct ggtgtccttc gggggcttca tttccaaaaa ggagaagctg
     4741 cacagacaaa attgattcgg gtagtcactg gagcagtttt agatgtagta gtggatatcc
     4801 gcaaaggtag cccgacttat ggtcaatggg acggatttat cttatctgaa cataatcatc
     4861 gacagttgct tgtacctaaa ggatatgccc atggattcgt tactttgaca ccaaatgtga
     4921 acttccttta taagtgtgat aattactata atgcagaagc agatgggggc atcgcatttg
     4981 atgatccaga tttagcgatt gattggccaa ttgatccatc aagagcgatt atgtcggaaa
     5041 aagatcaccg tcatccgaca ttaaaagaat ttgaagcaga aaatccattt gtttacggag
     5101 aaatctaaga attaaggaga aattttctta tgaaaaacat cattgttacc ggaggtgccg
     5161 gattcatcgg ctctaatttc gttcattacg tggtaaacaa tcatccagaa gtgcatgtca
     5221 ctgttttgga taaactgact tatgcaggaa acaaagagaa cttggccgga cttcctagcg
     5281 atcgcgtaga attggtcgta ggagatgttg cagatgcaga attagtggac cgtctagtaa
     5341 aagaagcgga cgcagttgtt cattacgcgg cagaatccca taacgataat tcattaaaag
     5401 atcctttccc atttgtacaa acgaatatca ttggtacgta tacactttta gaagcttgcc
     5461 gcaagtatga tgtacgctat catcacgtat ctactgacga ggtttacggc gatcttccat
     5521 tacgtgaaga cctgccagga catggggaag gcgaaggaga aaaattcact gcggaaacac
     5581 catataatcc ttcaagtcca tattcttcta caaaagctgg ttcagatctg ttagtaaaag
     5641 catgggtgcg ttcttttggc ttacgtgcaa ccatttcaaa ctgttcaaac aactatggtc
     5701 cttatcagca tattgaaaaa tttattcctc gccaagtaac gagtatctta agcggtattc
     5761 gtccaaaatt atatggcgaa ggaaagaatg ttcgtgactg gatccatacg aatgatcatt
     5821 cttcagcagt gtggctgatt ttgacgaaag gcagaattgg cgaaacctat ctgatcggtg
     5881 cagacggtga agaagataat aagaccgtga tggaattgat cttagaaatg atgggccaac
     5941 caaaagacgc atacgaccat gtaaacgacc gcgcagggca cgatttgcgt tatgcaatcg
     6001 attcgacaaa attgcgagaa gagctaggtt ggcagcctga gtttacgaac ttccgtgatg
     6061 ggttggctga cacgatcaaa tggtacgaag aacatgaaga ctggtggaaa aaagaaaaag
     6121 aggccgttga agcagcatat gccaaaaatg gacaataata cagaggaaat ttcactgtat
     6181 taatggaaaa acacatgtga ggcaggcgct cgcctgtctc ttttttaaaa taaaaatcaa
     6241 ggagcggata accatatgtt tttattaact ggaggaaatg gacaattagg tacagaattg
     6301 cgccatttat tagatgaaaa aaatctgcca tatgtttcaa cagatgcaaa agacttggat
     6361 atcacagatg ctgagaaaac aatggcttat attacagaat taaaacccga tgttattttc
     6421 cattgcgcag cttacacagc agtagataaa gcagaagaag aagcaaaaga attagatgag
     6481 aaaatcaatg tcgatggcac aagaaatgta gcacttgcag caaaagctgt gaatgcacaa
     6541 ttcgtctata tcagtacaga ctatgttttc gatggaaaaa acaaaaatgt agaatacgaa
     6601 gtagacgacc aaacgaatcc actaaatgaa tatggtcgta cgaaattatt aggcgaaaaa
     6661 gcagtagaag agattttaga agactattat atcatacgaa cttcttgggt tttcgggatc
     6721 tatggacaca atttcgtctt cacgatgcaa cgcctagcgg aaacccgcga caaactgact
     6781 gtagtaaatg accagtttgg gcgaccaact tggacacgta ctttggcaga atttatggta
     6841 tatgtcatcg aagaaaaagc accatttggt atttatcatt tgtcgaacga aaatagctgt
     6901 tcttggtatg agttcgcaaa agaaatattg aaagatacag atgtagaagt tgcacctgtt
     6961 acgtcagaag aatttccgca aaaagcaaca cgtccgcagt attcagtcat gagcctgaaa
     7021 aagacagaag cgttaggttt tgtcatacca acttggcaag aagcacttgc gcaaatgctg
     7081 gaagctgcca aaaaaatgaa taagtaattt cctttttatc aaatagaatt tggagcatga
     7141 tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag ccaaaagcga atcaggcaga
     7201 ttcgaatttg gtgcttgacc tgtttctaaa actccgctat cttattggcg ttttagtcat
     7261 cgtggtcatc gttactttta atttaaacgg aagttcactt ggtgtttggg ataaatatgt
     7321 gtcgcaaaga gacgatggga aaaaaactga tgtgattttt ggacagaatc gggaagtgcg
     7381 gtcagacgaa tggctggtgc agacaccttt ttacctctca caagctgaag aggacttttc
     7441 gttggtgaac gaggattatt cattaaacgg acaaaacatg atcattgcgt ataattctcc
     7501 tgtaaaggat attacggtta tcggaaaacc attcaactgg ggattctttt tcctcggaag
     7561 agatcgagga ctttcctttt attgggcgat gaaattagtc gtcatggtct tgcttggatt
     7621 cgaagtactg atgatattaa cgaagaggaa aaaagctcta tcgctaatcg gtgcatttgc
     7681 gctgacattt tctccagcag tccagtggtg gttcatgcaa catgtgggcg atttgatttt
     7741 ctttactttg ggactgatga ttgcttttta tcattatttc tacaatcatg ataaaaaatg
     7801 gctacgaacg ttgatgatgc ttttggtggt tattttcggt attgggttta ttttagtgat
     7861 ttacccagcc catcaagtga tgctggcgta cttgctgatt ttttattttg ttggcctgct
     7921 tatttactat agcaagaaag tttcatggga tctgttggat gtagtgttga ttggaggggc
     7981 agttttattt attggcggag tcatggttca tttctggcta acgtcaaaag acgcattgtt
     8041 agcttctttg aataccttat atccaggaaa acgggtaagt actggaggaa agtggacgat
     8101 tggcgaattt ttttctttcc ttacaaattg gaaaattcct tttgaagata tcaactattc
     8161 gaataactct gaagtggccc tgttctatca cttttttcca actgtattcc tagcaagtcc
     8221 atttgtgtta tttggcaaaa aagatagcga acaaaagtta ctgggacgta ttttgatgat
     8281 tttttgtctg ttttctatct tttggattac cgttgggttg ccaaaatggc tagcagaaat
     8341 cactctgctg tcttatgttc ctccattaag agccatcctt acttttagtt ttgcggcttg
     8401 tttattatcg atctggttta tcgcttatat ttggagagaa gatgttattc ctatttggta
     8461 taaattcatt ttgttggcag taaatgcatg gtcttatttc tatgcgatta cgcattcgaa
     8521 aatggataat tatttttcgg attttgaaac catcgcagtt gctgtgttgg gaacagtgat
     8581 tttagctttt gcattatttg gctggagacg cttgttttac ctcgcttttg tcgccttagt
     8641 tatcgtatct ggctttttcg tcaatccagt cgtagaaggt acaggagcga tttttgaaaa
     8701 aacactggca aaagaaatcc aaaaaatcga tcaacaagat cctggtaaag tttggttgtc
     8761 tgaagatgaa ttatataact tcaccccaac attgggagtg aaatccttca atacagtacg
     8821 cttttatcca gatatagctg catggaagaa aatcgatccg aaagaagaat acgagaagta
     8881 ttataatcgg tatgctcata caagagcttt tatcgctaag gaagatacaa agttcatttt
     8941 agatcgtcca gatatgtttt cggtcacgtt gaattttgaa gacgcagaaa aattagggat
     9001 cgactatgtg atcagtaaac gaccattaac tgattacaac caattatatg aagcgcagtt
     9061 cacgcagcta tatggaccgg ataaagacgg ttatatgatt tttaaagtag aatatccgga
     9121 ttatccagat gtaccacaaa cacaaactca ggaaaatcag ctagtcgtac agtaataggc
     9181 tagctgagaa taatcctgaa atattagtag aaaggagagt gaagataatt caagcaatcg
     9241 tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct tttgttgctg acgctagtct
     9301 tttttgtttg gacagcaggg gtaggttttc cagcaacttt gtttatatat aacaatagtt
     9361 cattacttat cgcttttagt gtggctattt tgctcatttt aaatgtttat aaagcaaaag
     9421 gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt cattttcttt tcttactttc
     9481 gggaattaag aaatgagtct ttatttggag acttactgtt tcctttggtt attttatttt
     9541 gtttagtaat taaatggatc gagtggacaa aatgggatcg cggtatttat ctgattgtct
     9601 ttacggtctt cctcggaatc acgttatatc gtgtatttac agagatccaa gtaccagaag
     9661 gacaaagcat ctgggctcca ggcaattcgc tttctgatat ttggatcaat acgaatacaa
     9721 tcggaagttc tttgatgact cttgctttat tgatcagtgg atttgccagt tcttttgaac
     9781 gatggtatgt tcgaatcatc ggtgggatag cagtcgttgc agccttttta ggtatttggg
     9841 tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt tgcgatattg gatcttttac
     9901 ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc ctacacggtt acgattcttt
     9961 tagctttgcc gatttcttat ctggcggctg tctctgaaaa agtcaatttg tttaccgatc
    10021 gagaagatat ttggcataaa ttctatcaga cgctaggaga aaaatctgaa caagttttac
    10081 taggaatgaa aacatttatc ttccagcgag gaaaccaatt tttaggaaac cacaatagtt
    10141 ataattctat tttgaatatc tatggattga taggatttgg gattgctgct ttgctgctta
    10201 tcctttttat tggtcgctta acgttgaaag cagatttatc caacgggcag atgactttta
    10261 tctgggcctt ttttgcggtg atgatgcagt catttatgga ggatacattg acttcatacc
    10321 catgggttcc gattatttat atcctgttgg ctatggccgt tcatcgatat gatgaaccag
    10381 aagttgcgga ggaattctat gttatggaag aggcatatga cgatatgact tatgatgata
    10441 catcttatga ggaacaacca gatagcgctc attcttccag gatccaacgc tatcattgat
    10501 aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc cttttttggt ttattgcaat
    10561 tgtctagcat cttctgtaaa atagaaacgt tagagaaaat cttgtatcat agaaaattaa
    10621 gaaagaaata agaacgcagc tcggtaccaa aaatatggta aaatggcact tgactgtaat
    10681 tcactgaaaa tcgcttggag gaacacaaat gtttaaagat ttgtatttat ttatgaagga
    10741 catttatcag aacagaaaat tattgctaca gttttcgatc aatgacttca aatcaagata
    10801 tgcaggatcg tttttaggaa tcttatgggc gtttatcaat ccgctattta ctgtattgat
    10861 ttattggctg gtatttggtg ttggactgaa agcacaaatg atcgatgcaa aatacccatt
    10921 tatcgtatat ttagttaccg ggatgattcc ctggttcttc ttctcagatg catttgcatc
    10981 aactacggtt gtttttcggg aatatactta tttagtaaag aaagtcgtat tcaatattcg
    11041 tatcttgccc acgacgaaaa tcctatccaa tctgtacacc catttattct tcattctgat
    11101 tggcatagct gtcgcgctta taaatggtat atatccttca gcaatgtctt tacagctgat
    11161 ttattattta ttttgcatgt gtgctttctt gacaggattg acttggctga cggcatcgat
    11221 ccaaccattt ttacctgata tcatgcaatt aattacgatc gtgctgcagt tgatcatgtg
    11281 gacattaccg atattatgga gccctaatca gtttgattca ggtcttgtaa gcatattgaa
    11341 attaaatccg ctgtactatg ttgttcaagg ttatcgtgag tcatttttaa gtgaagcttg
    11401 gttctgggag catggaacat atactttgta tttctgggtt ttcacgttag taatgttcgt
    11461 aatcggctct gcagtgttcc gtagattgaa accacatttt tcagatgtgc tgtaaggaga
    11521 atagctatga cagaatatgc aattagacta aaagatatca caaaatctta taatatgtac
    11581 gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt ttaagaaatc ctatcatgat
    11641 gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa aaggtgagat gatcggtttc
    11701 gtgggagaaa acggatcggg aaaatctaca atgttgaaaa tcattacggg agtactgaca
    11761 ccaacatccg gcacgatgga aatcgaagga aaaatctcag ctttgctaga actaggatca
    11821 ggattcaatc ctgagtattc tggctatgaa aatatctatt taaatggaat ggttctagga
    11881 ttttcaagag aagaagtcga tgaaatggtc gatgatatca ttgaatttgc tgacatcgga
    11941 gaccatattt atcaacctgt aaaaacttat tcaagcggga tgttcgttcg tctagctttt
    12001 gctgtagcaa tcaatgtgaa tcccgatatt ttgattgtgg atgaagcatt ggcagtaggc
    12061 gatcttgaat ttcaactaaa atgtatggaa aaatttacag aaatcaagaa ctcgggaaag
    12121 acgattttat tcgtttctca tgatatcagt tcgattcgtc gtttttgtga tcgttcctat
    12181 tggttgaaaa acggagaagt cgtcgaatat ggcgatacga tggaagtaac gacgaattat
    12241 gaaaactttt taaagaaaaa atcagttaaa acagtggatc ggaataaaaa ccaagtagaa
    12301 cggttcgccg ctccagatat cgtcgacgtg acgaaagcgg aattgttgga taaagacaaa
    12361 aatccggtgg atatgctgga acaaggagaa aaactatacg ttaaagtaac ttatgaagta
    12421 aaagatgata caatcaaaaa tccggtttta ggaattgcgc tacgaacagt cgacaatttt
    12481 tatgtatgcg gacttaatac tctactagat ggtatcaaga ttccttggaa aaaaggagag
    12541 aatatctttt atctggagta tgaaaagtta ggtttgttag ctggtgaata ttattttgac
    12601 gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat ataagacaaa gtacatgaac
    12661 ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg tcttagacca taaatgggaa
    12721 gaagggactc atagcaatga aatatgattt tgaaatggac ctagatgaac aaagcagtgt
    12781 gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt ttagagtttg gtcccggaaa
    12841 cggccgatta acgaaacatt taatcggagc aaaacaatgt gaagtgagca ttgttgagtt
    12901 agataaggaa ttgttcgatt ttgtcagcga atttgctcaa gatggatttt atggtgatat
    12961 cgaaagtttt gagtgggcaa actattatgc tggacagaca tttgattacg tcttgtttgc
    13021 ggatgtttta gaacatttgg tagatcctgg aaaaacattg aagaaagtca gagaattttt
    13081 gaatgaagaa ggagagatcc tgattacatt ccctaacttg gcgcataatt ctgtcatgat
    13141 cgatttgttc aataaccaat tgccgtgggc ttcttatggt ctcctagatg aaacccataa
    13201 ttctttttat actcatgatg gcttccaaaa agtttttgaa aaagcgggat tattcattaa
    13261 tattgaagat tatttgtact tagctgtagg tgatacagaa ttaaaatcaa cttatgaaga
    13321 attgccggaa gctgtacgct atgatttcaa aatgcgacca tttggtgaag tctaccaata
    13381 ctttttctca ttgatgaaac atccagtagc acaaagttcc attgcagagc cacaaaattc
    13441 taattatgta aaagtgttag aagtaaccca gcaaacgaaa caagatgaaa caatccaaca
    13501 aataccattc aataatttca caggggaaaa tgagacacta agtttccccg tatcagaaac
    13561 aacagaaaga atggtgtttc gcttcgcgca acaacctagc tttatcgaat tcagcgcaga
    13621 agctgccgga gaaaaactaa catttatcga ttcgaatgct gtagtcaaaa cagtaaatga
    13681 ttgttatttg tttgatggga aagaattacc agagttcgtt ctaacggata tttctgggaa
    13741 agaagtgact atccattgtc actatcgctt tatcggggaa ttgacaccaa caatgaaaga
    13801 attgttggaa acgattcgtc ccatggctga agtcacaaag cagctttctg agaaaaatga
    13861 cgaattagaa agagaaaatc atcatcttct tgaggaaaat acaagactag accatacgtt
    13921 aaaaacaacc acaaatcgct attgcacatt gttggaaagt gatgaatgga caataaaaca
    13981 tagactagga cgacgtaaga aagaaacttc gaaaaaaatc caagaaaaag aactttctat
    14041 atgtattgac gagaaaatct gggatgcaga aacaaaaata ttaaaaatca tcggatgggg
    14101 aatcgctaat tcggatcgcc agcccttgtc ctataagtta tcagctgacc aatcgccatt
    14161 tttcgaagca attcctgttt cacgagaaga agtcaatcaa gcagaacagc tcgcaaaagg
    14221 aacagaagcg ggatttgagt tacgaatcct ttgcgagcaa gaacgttcat tcttagtgga
    14281 agcagtagct caaaatggcc aatcatggat cattgaaatg taaataatga ttaacttata
    14341 aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg ccgtatttat cgatcaaatc
    14401 actcgtgaac gggccactgg cgatttgctt attgtgggct gggcaattga tgaagtgacc
    14461 aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga ttgctgaagc aacacatgtc
    14521 gtaagattgg atatcaatca tttatataat cttgatgtta agacccaaag cggtttcaag
    14581 gtacgtttat cagggaaaat gagaggaaaa gccatcttag acttccaaac agcgagacat
    14641 caaaatggca tagctgtgaa attaaacggt aaataccctt atgatgacgg cattgaatct
    14701 tcatgggaaa gaaaaaaaag attactgaaa aaagggataa attatgcaag gactcatggc
    14761 gtaaaaaaag cgatccgtcg ggtcaaattg gaattgaagc caggatcgat cgactatgca
    14821 gagtggatca gtcgtcatga gaaaccagat ttgaaagagc aaaggaaaga agcacaagga
    14881 tttgcgtacc gtccattgat ttccatactt atgcctgtct ataacgttga aatcaaatgg
    14941 ctcgaaaaat gcattgattc ggtactggat caaacgtatg atcattggga actatgtatc
    15001 agtgatgatg cttctactga tccagctatc cgaaagtgtc tggaatccta tcaagcaaaa
    15061 gatgatagaa tcaaagtcgt tttccgaaaa gaaaatggac acatcagtct ggcaactaat
    15121 tctgcattgg aaatagcaga aggagaattt atagcactct tagataatga tgatgaatta
    15181 ccgccatttg cattatatga agtggcaaaa gtgctaaacg tacatccaga actggatgtg
    15241 atctacagtg acgaagataa aatcgatgca gatggcaatc gctttgatcc gcatttcaaa
    15301 gcagactggt cgcctgatac attgatggga aataattata tctctcactt aggcgtatat
    15361 cgaacaagca ttgttaaaga acttggcggt ttccgtaaag gctatgaagg ttctcaagat
    15421 tatgatttgg tccttcgtgt gacagaacaa atccctgcag atcatattta ccacattgac
    15481 cgagtcttgt atcattggcg gacgatccca ggatcaactg caagtaatgg cgaagccaaa
    15541 agttatatct atgattccgg agtgaaagca ttgacagatg ccctgtctag aagaaacatc
    15601 aaaggaagcg tacgtcctgg cagaatttct ggattttatg agattgctta tgacgtcttg
    15661 caagaggatt tggtcagtgt gatcattcca acgaagaatg gctatgaaga tctaaaaact
    15721 tgtgtggatt cgatcatcga aaaaacgtct tattcaaatt atgaaatcat tattgcggat
    15781 aacggaagta cagatccaaa aatgcaagaa ttatttgcag aatacaagca tcagctgaaa
    15841 gaccgcttta tcgtagaatt gattgacatt ccattcaatt attctcgaat taacaattta
    15901 gcagcagaaa aagcaaacgg caaatacttt ttattcttga ataacgatac agaagtgatt
    15961 gagccagatt ggatgacggt gatggtttct tatgcacagt ttgatcgaat cggctgtgta
    16021 ggagcaaaac tgttttatcc agatgataca acgcagcatg caggagtatt attaggaatc
    16081 ggtggtgttg cagggcatgc attgaataac tatgatcgaa cacattgtgg ttatttcggc
    16141 cgattggtca ttgacgtcaa ttacttagca gtcactgcag cttgtatgat ggtcaaggca
    16201 gctgacttca atgctgttaa cgggtttgat gaaacattag aagtagcatt caacgatgtt
    16261 gacttgtgct tgaaagttta tgaactgggt cgttacaatg tctatgcaca tcaagcagaa
    16321 ttgtaccatt tcgaatctaa atcaagaggt tatgaagata ctccagaaaa acaaagacgt
    16381 tttgctggtg aaatcaaaaa aatgcaagat aaatggccag catatattgc gcatgatcca
    16441 ttttacaatg ataatttaac aaaagacgga atcggcgact tttcgttgag accagattaa
    16501 actataataa aaagctgtta tgttgtgatt cgctcgcaca taacggcttt ttatagtttt
    16561 ttgtcaaaaa aaggcagaag gtgccgactc aaaaaaataa gtcgaggttg ggacagaagt
    16621 gtttaactcc gagaaataag aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    16681 tcggcttatt taagaagaag ttgcttctgc tcccaccgtt tattcgtttt taaagaatga
    16741 gacaaaagtg tttcttactt ttgtcccacg ctcttattca tgctcagagc gccaatagat
    16801 acaaaatcat agccaaagtt tcacgaaccc tagctgtcaa agaatcgata ttttatttta
    16861 atataggcta ttttaaaata aatggtttaa ggtattcctc tacaaaagcc actctatcaa
    16921 ttatcatggg tttaaaataa gaagtgatgg agataaccag attgttctta gcgtcaatat
    16981 aaagaacatt tccactatta ccaattgctg aatatatttc gttctcagaa tctaataccc
    17041 accataaata gccatatctc atattttgta agtcttctaa agcactggtt ctaggtttga
    17101 acatctcgtt tatccacatg ggtggtacga tttgttttcc tttatacatc cctttattta
    17161 ggcataatac accaattttt gccagatcac ttgccgataa acacaaacca tatcctgccg
    17221 tcccgatatt tagtggatca caaaaccaga cattggtttt aggagactta tccattgtaa
    17281 actgcttatg ttcttcagct gtttcagcat aataattgac atggtttggt atccctatag
    17341 gatcaaatag atacttattg gcaaaatcaa cagttgttag cttacttgtc tgatacaaca
    17401 agccagttaa tatgtgtaaa cagacagttt gatagttgaa ttcgccggtt ataccttttc
    17461 ttcctcccaa taaatctagt gatgtttttg tccaatcttc acttgaacag acttttgtcc
    17521 atggatcgcc tttacatttg tagggtgctt tcattgttag caagtgcttg attgtaatag
    17581 cgtaaatagt tttctccccg cgtttcactt tatactctgg aaaataatct aatactttat
    17641 catcgatact tttgattaat ccttgctcta aagcaatgcc aatcagcaaa gatacaatac
    17701 ttttcgttgc tgacatgata tgacaagtat cgtctttttt ataaccattc cattcatctg
    17761 tataaacttc taccccatcc ttatatgcga caatttgaca tatatttggt tgtcgaaaac
    17821 caataaaatc atgcaactct tgtttattca taagatatta cctcactttc ttctataagc
    17881 ataatgtcat taaaaatcga tttcaatttt ttcgattctt cacgtaatac aggtattctt
    17941 atggactaga cagcgctttg ggaagatgga ttctctgaaa aatataaagg ttcactttat
    18001 gagtggataa aaaaagcagc aaatacttcg acttctttaa tttaccaaca ttagtgaaaa
    18061 tcgattcatc tggagatttg taatttagag tccttgtctt gattagaggt gtataaagct
    18121 ttttattaga catttttaaa ttccattaca atttgcttat aaactaggtt tttaaaggtg
    18181 actatggtaa gataatagag atattatttt gtgattagga gtgtatatat gaataaaaat
    18241 ggagaatgga gtgctgcacg acggatattc attatattaa tggatgttat tgtatacaat
    18301 gcagcaatat atagtagttt cttactgaag tttcgtggag agatcccatt acgaaattta
    18361 gaaactttcc aacactcagc aattattatt tcagtgattt ttattgcgtt gaacattttg
    18421 ttgggtgcat atgtttttta taatcgaatg atcagtgaca tcgtgtttgt cacagtcatc
    18481 ggtcaggtat tgatgacact agggatcatg gtagtgactt ttgcgggccg ctggtttgct
    18541 tttccacgtc ttgttatctt actttcattt gttctcggta cgcttttctt aagtatctat
    18601 cgtattctcg tatataaact ttatttacgc gtaagtcgag ataaaaaggt cgtcattatt
    18661 gggttagaaa aggatgtcgc accagcaatc caaaattttg gttcaaagaa aaacaacaaa
    18721 cataaagtcg aagcagtcat tcttgataac tattatgcaa acatcaaaaa tatgattaaa
    18781 gacatcgata ttgtctatct tgcttctcat atcgaagaaa ctgaaaaaat caaaatctat
    18841 caattagtta ctaaaaatga aaaaaaattg tttttgaata caacatttga aaacttgaca
    18901 ttgatcaatc caaatattat gaatttcgaa gatgaaagta ttattgaagc atctggattt
    18961 agaatccaac ctgaatatga actattcaaa cgtttgtttg atatcatcgt ttcacttgtt
    19021 ttattagtta ttgcttcacc atttatgtta ttcacagcaa ttttagtcaa agtgacatca
    19081 ccgggaccaa tcatctataa acaagtgcgg atcacaaaaa atcaaaagga attttcaatc
    19141 tataaattcc gctcgatgac cgccacagcc gaagcaaaat ctggaccagt tttagctaag
    19201 agcaatgatg cacgagtaac gcctgtaggt aaatttattc gagctgtccg ctttgatgaa
    19261 ttaccacaaa tcttcaatgt gctgaaaggc gatatgtcga tcgtaggacc acgtcctgaa
    19321 cgttctttct tcgtcgatca gttcaatgca gaaaaccctt attactactt acggcataat
    19381 gttcgtgctg gtatcactgg gtatgcacaa gtctatggaa aatatgtctc tgattacaac
    19441 agtaaattac gttttgattt actttatatc aaaaaatatt cattgatgct tgatttaaag
    19501 atcttgctgc aaaccatcaa gatcctattt gataaaatgt cctctcaagg acttgaagaa
    19561 gacgaaaatg tgatctcatt ggcagaaatt gcttttccga atgaaaaaat ctttcgctag
    19621 ttgggaggcg taaaaatgaa caatccgaaa ttcagcataa tcgtaccagt atacaatgca
    19681 gaagcaacta ttggtcgaac gattgatatt ctaaaagaat tggattatga agagtacgaa
    19741 gtcgttttag taaatgatgg atcaacagat caaacacagg aaatcattca agaactgatt
    19801 gaaaatgatg aacgttttga attgattact acgaccaatc aagggcccgg attagcaaga
    19861 aacgaaggaa taagacagtc aaaaggagaa tatctcttat tcttcgatgc agatgacacg
    19921 ccagtaaaaa cgatcttgtc agattatgat catttgctag aaaagagtcc aaaagcggat
    19981 cttgttgtta gttcgtttgt ttttcgtaca atggacgagg gaagaatcgt ttccgaaaaa
    20041 gaaaacttag ttacagaatg tcactatcta actcacgaag agtttataaa agacatgtat
    20101 cagttaatga atgaacagtt gatgtatgtt gtatggaaca aatgctatcg tagcgatatt
    20161 ctgaaaaata accaaattct atttaaaact tataatagtt gtgaggatcg aatatttaac
    20221 ttacattatt atgaatactg tcatcaagtg atcatgaacc caaaagtaga atatatttat
    20281 gaatttgaag gtgggaaggg gatcactaat caataccgac ctaacaagtt cgaaacattt
    20341 aaagaattct attcattagc aaatactgta acagacgata ttaataaaga cggtatggcg
    20401 gcgcttcttt taaaagggac aacttcagtt attttttcta tttatagtac ctctgcaaga
    20461 acttcaaaag agaagaaaga cgaagcaaag cagatattga gtgatccagt gatccgagaa
    20521 gccaagcaaa ttgctaagac ggattcttca gcaaaaaaaa taacaaagtt gctttacaat
    20581 atgccagctc cactattttt atctgctgta aaagttggaa gcttcgttga agtgaaaatg
    20641 ccagggttaa tggctgcttt gaagcgagta tattagttaa tggaggaaaa tataatgaaa
    20701 agaattttgc attttcaggg acgaatgggt ttaggtggag cagagtcttt catgatgaat
    20761 ttatatcgta aaattgatcg tacaaactat caatttgatt ttttgattta tgaagattat
    20821 gcagatgttc aagactatca ttcagagatt gaaagattag gaggccgtat ttttgttgta
    20881 cctaatccta agaaaaatat ttttaaatat ttaatagaag tgaataagtt actaaaaaaa
    20941 gaatcttttt ctatagttca caatcaggtt tattttggcg ggggaattaa tttatggtta
    21001 gccaaaaaga atggaatcag acagagaatt gctcatagcc atgcaactga agatggaaaa
    21061 tctcaaaata ttgtaatgaa tgtattgcgt aaatttttaa ccaaattgtt gttacaaaat
    21121 gcaacagact accttgccgt tagtcaagaa gctggtgaaa gtctgtttca aaaccatccc
    21181 tttgagatag ttcataatgg tattgattta gagctttata gtaaaaattc agaagctaag
    21241 gttaataaga gaaaagaatt ggatatctca atgagtactt ttgttgtggg aaacattgga
    21301 cgattagaga aacaaaaaaa tcaatcttat ttgttagaga tttttttaga aatactaaaa
    21361 atgaattctg attcttactt gattttagta ggtgaagggt cacttagaaa taaactgaaa
    21421 gccaaagcag tagaattgga tatagagaaa agagtaaaat ttttaggaga aagaaacgat
    21481 attcccgagt tattatctgt gatggatgtt tttgtaatgc cttctcttta tgaaggtctg
    21541 ccaatggttg gattagaagc acaagcagca aaattaaaat tagtcttagc agatactatt
    21601 tccaaagata caaagttaac aaataatgtg gattttgtat cattagatga aacaccacaa
    21661 gaatgggcaa aaaaagtaat aaaagagccg tatgctaata aaataacaca agaactaaag
    21721 agttatgatg tttcgtacac tactgaacaa atggagagta tatatagtag agatcattaa
    21781 aaatgttcaa gatattttag gagtgattat ttttgaaaat atgttatttg atattggcat
    21841 acaatgatat tggcaactta aagagattgg tcaatcgatt gaacgaaaat gcagttttta
    21901 ttattcatat agataaaaaa ac
//
LOCUS       DAYYDS010000045        21340 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000045 DAYYDS010000000
VERSION     DAYYDS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.045"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..152
                     /locus_tag="ACHA02_001662"
     CDS             <1..152
                     /locus_tag="ACHA02_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8669992.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ACHA02_001663"
     CDS             complement(230..763)
                     /locus_tag="ACHA02_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8669993.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACHA02_001664"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACHA02_001664"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8669994.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACHA02_001665"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACHA02_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8669995.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ACHA02_001666"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ACHA02_001666"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8669996.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ACHA02_001667"
     CDS             complement(4488..5066)
                     /locus_tag="ACHA02_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8669997.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="ACHA02_001668"
     CDS             5388..5858
                     /locus_tag="ACHA02_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8669998.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="ACHA02_001669"
     CDS             complement(5966..7192)
                     /locus_tag="ACHA02_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8669999.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="ACHA02_001670"
     CDS             complement(7946..8146)
                     /locus_tag="ACHA02_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8670000.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="ACHA02_001671"
     CDS             complement(8280..8888)
                     /locus_tag="ACHA02_001671"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8670001.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="ACHA02_001672"
     CDS             complement(8973..9443)
                     /locus_tag="ACHA02_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8670002.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="ACHA02_001673"
     CDS             complement(9491..10405)
                     /locus_tag="ACHA02_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8670003.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="ACHA02_001674"
     CDS             10551..11354
                     /locus_tag="ACHA02_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8670004.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="ACHA02_001675"
     CDS             11532..12479
                     /locus_tag="ACHA02_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8670005.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="ACHA02_001676"
     CDS             complement(12547..12840)
                     /locus_tag="ACHA02_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8670006.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="ACHA02_001677"
     CDS             complement(12868..13533)
                     /locus_tag="ACHA02_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8670007.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="ACHA02_001678"
     CDS             complement(13673..14305)
                     /locus_tag="ACHA02_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8670008.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /gene="liaS"
                     /locus_tag="ACHA02_001679"
     CDS             complement(14312..15379)
                     /gene="liaS"
                     /locus_tag="ACHA02_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8670009.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /gene="liaF"
                     /locus_tag="ACHA02_001680"
     CDS             complement(15376..16107)
                     /gene="liaF"
                     /locus_tag="ACHA02_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8670010.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ACHA02_001681"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ACHA02_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8670011.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ACHA02_001682"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ACHA02_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8670012.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="ACHA02_001683"
     CDS             18270..19439
                     /locus_tag="ACHA02_001683"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8670013.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..>21340)
                     /locus_tag="ACHA02_001684"
     CDS             complement(19706..>21340)
                     /locus_tag="ACHA02_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8670014.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6585 a         4179 c         3365 g         7211 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataatagta
    20221 taaaatttct tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata
    20281 ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat
    20341 ttaaaggagt attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata
    20401 ttatacccat tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta
    20461 gtataattaa acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta
    20521 tacttccaat caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat
    20581 aaggtctatt cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg
    20641 aaagaagtaa agtaactata gcagctttta ttaatttaat aattaagaag atttctattt
    20701 tctttttccg ataaaattga ataattaaag caattataat cattaccata gtcatagcta
    20761 cagaaagaac atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt
    20821 ctttttttga gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg
    20881 cgagtaattc ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg
    20941 tatatataat agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat
    21001 aatgacatat aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg
    21061 ctaaattagt aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat
    21121 tatttacttg catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag
    21181 attctaaact taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca
    21241 tgtttttaaa tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt
    21301 ttacagaaaa aatttttttc aaattcatat accacctcat
//
LOCUS       DAYYDS010000046        19673 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000046 DAYYDS010000000
VERSION     DAYYDS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.046"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..390)
                     /locus_tag="ACHA02_001685"
     CDS             complement(<1..390)
                     /locus_tag="ACHA02_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670015.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLN"
     gene            complement(407..1612)
                     /locus_tag="ACHA02_001686"
     CDS             complement(407..1612)
                     /locus_tag="ACHA02_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8670016.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            2306..2692
                     /locus_tag="ACHA02_001687"
     CDS             2306..2692
                     /locus_tag="ACHA02_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8670017.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            2786..2977
                     /locus_tag="ACHA02_001688"
     CDS             2786..2977
                     /locus_tag="ACHA02_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670018.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            3491..3823
                     /locus_tag="ACHA02_001689"
     CDS             3491..3823
                     /locus_tag="ACHA02_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8670019.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            3733..3927
                     /locus_tag="ACHA02_001690"
     CDS             3733..3927
                     /locus_tag="ACHA02_001690"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8670020.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            3966..4112
                     /locus_tag="ACHA02_001691"
     CDS             3966..4112
                     /locus_tag="ACHA02_001691"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670021.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            4550..5890
                     /locus_tag="ACHA02_001692"
                     /pseudo
     CDS             4550..5890
                     /locus_tag="ACHA02_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(6131..7747)
                     /locus_tag="ACHA02_001693"
     CDS             complement(6131..7747)
                     /locus_tag="ACHA02_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8670022.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            7865..8083
                     /locus_tag="ACHA02_001694"
     CDS             7865..8083
                     /locus_tag="ACHA02_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670023.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            8278..8739
                     /locus_tag="ACHA02_001695"
     CDS             8278..8739
                     /locus_tag="ACHA02_001695"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8670024.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(8932..9171)
                     /locus_tag="ACHA02_001696"
     CDS             complement(8932..9171)
                     /locus_tag="ACHA02_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670025.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(9195..10718)
                     /locus_tag="ACHA02_001697"
     CDS             complement(9195..10718)
                     /locus_tag="ACHA02_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8670026.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(10736..10858)
                     /locus_tag="ACHA02_001698"
     CDS             complement(10736..10858)
                     /locus_tag="ACHA02_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8670027.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(10888..11871)
                     /locus_tag="ACHA02_001699"
     CDS             complement(10888..11871)
                     /locus_tag="ACHA02_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8670028.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(11895..12044)
                     /gene="rpmG"
                     /locus_tag="ACHA02_001700"
     CDS             complement(11895..12044)
                     /gene="rpmG"
                     /locus_tag="ACHA02_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8670029.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(12065..12442)
                     /locus_tag="ACHA02_001701"
     CDS             complement(12065..12442)
                     /locus_tag="ACHA02_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670030.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(12474..12653)
                     /gene="rpmF"
                     /locus_tag="ACHA02_001702"
     CDS             complement(12474..12653)
                     /gene="rpmF"
                     /locus_tag="ACHA02_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8670031.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(12668..12937)
                     /gene="rpsN"
                     /locus_tag="ACHA02_001703"
     CDS             complement(12668..12937)
                     /gene="rpsN"
                     /locus_tag="ACHA02_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8670032.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(13460..15226)
                     /locus_tag="ACHA02_001704"
     CDS             complement(13460..15226)
                     /locus_tag="ACHA02_001704"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8670033.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(15186..16939)
                     /locus_tag="ACHA02_001705"
                     /pseudo
     CDS             complement(15186..16939)
                     /locus_tag="ACHA02_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(17049..17618)
                     /locus_tag="ACHA02_001706"
     CDS             complement(17049..17618)
                     /locus_tag="ACHA02_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8670034.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(17636..19051)
                     /locus_tag="ACHA02_001707"
     CDS             complement(17636..19051)
                     /locus_tag="ACHA02_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8670035.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(19062..>19673)
                     /locus_tag="ACHA02_001708"
     CDS             complement(19062..>19673)
                     /locus_tag="ACHA02_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFM8670036.1"
                     /translation="IFLGKGNIFQVGCFIIATVLVLMFRKIKTCIYFILIYSVLFLVM
                     KIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVYTSNELLSSLEKIHLPKKIM
                     LSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQYLEFLLVPVLIRATMIAEEM
                     TANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFLSILI"
BASE COUNT         6463 a         3672 c         2850 g         6688 t
ORIGIN      
        1 gtttaacttt tctgaaaaag ttagacccac gtaataatct tcccagcttt ctctttcaat
       61 taactgatcc aatttttgat aatccataaa ttcatcatgt atcaaaaaga ataaataact
      121 tgatatttta gcatctagct taatcagatt aaataatgtt tgtttaaaat tgctaccatt
      181 aatatgactg atagattctc tagcttcttc acataaactt tttactttat taattactac
      241 tttgtcatat tcatttaaca cactattatt accattactt agacgctcag attctacgaa
      301 acaactcaaa tcagaaattt cccaaaaaaa ttttttgtta aaatccaatt tcctcaactc
      361 ctccaaacta cgtaaatgat atagtctcat aataaaataa aacctctcag ctaatgttgt
      421 tgaaattgtc cactaaacct aattgatatt gcttaggaga tacacctgcg tactttttga
      481 atactctcga aaagtgtgaa gaatctgaaa atcctaaacc taaagaaact tcactgatag
      541 tatggtttga tttcaacatt attttagccg tagaaatttt tcgcttagag atataattat
      601 aaatcgtaat atgcatttca ttcttaaagc tttctcttaa tttagtttca cttacagaaa
      661 aataattagc tatttccttt acagtaattc ttttatagat atttaagccg atgaattgaa
      721 cgacagaaga aattaaggga gaaagatgct ctatcttagt ttttccgatt tccttagtat
      781 aataaacaat cccagaatcc ctcactttta agacctcagg taacgataca gctaattcgt
      841 tttcttgtat caaagcatct ctactttgat acgcttctat tatatccatt cctaatgtta
      901 tagacgtttg tgctaacttt tcaaaaacaa tgatagaata atttttttca gaccgaatcg
      961 tatctccact aacacttgga attattccat tactcagatt aaaaaccatg ttttctaaat
     1021 ttcgaatatc tccattttta acatattcca gtattttatt ttcataaaga aacagatata
     1081 tttctggatc gtaattttgt gataacagat tatctatttt gatttgctcg atagttttac
     1141 ttagattttc tgtatattca attaacggtt catggaatag atcttctatt tttccagtaa
     1201 aaaaataatg aactaaaatc agaatatctc gaatgtctcc taaagaatac aaagtcaaat
     1261 tttgcaatga attatagagt cgttctctat cagccattgc aacattcaaa ttgttcattt
     1321 ttagtttaaa aaaaacttta tcaatattat ttgctctaaa tggaccaaat aagtaatatc
     1381 tggttttaaa catgtataga agaaacatct cattaaaatc accagtaaag aaatggaaat
     1441 ctttagtttt tttaatatta tcagttagta actttttata atcataaaaa aaataagtag
     1501 ttctatctga gcgatactct tttataatac tgaattcgga attaaaaacc acgataggta
     1561 actttaaaca aacatgaaga gactttaata agacttcttt atctgaaaac acttttgttt
     1621 ctctctttcc tttctacatc attaaatagt attttatacg ttatctaaaa tactattcct
     1681 ctgtcaaaat aagctaactt acttcattat aaaatatcca cttatcgctt tcatgtatat
     1741 tccaccgtaa aaagtacatt ttatcaccga gaactcatca tttttcatca tgatgaatac
     1801 tatttacata tatctagttc aaagacaata attattatga tccgttatta tctaagcact
     1861 atacagataa taggaatttt tcgtttctaa atattccaat ttgggaatcc aataaaaatt
     1921 ttcgttcaaa tacgtgtcta ttcgaactct tttctagttg ttaattatga agggggaaga
     1981 aataaaaaat acaatctata cacgaataag aatattatcc aaaaggaggt tttagaaaag
     2041 atttattata cgtaaaaagc tcaagacatt ttcttagcca taaggacatg tttgatattt
     2101 aagaagatat atgaattttc ttttctaagt ataaagataa ctatccatta tcaactaaaa
     2161 aaacattttt gaaaataaac atcagcagtc ctatcaatgc atgtgagctt atagaagtta
     2221 ttcgcagcgc agcataattg cattagcttc ttttcgtgtt ttttgcctct tttcccttta
     2281 aatcttaatg gaggaggtgt gcaatatgtc agataacgac gaacgcgtaa atgcccagtt
     2341 gattcgattc tttgaaaagg tcattttgaa caccgctaat acttattata aaaaacaaga
     2401 agaaattcaa aaacatgaac aacttgatca actcccacca tatttgttta ctaaagatac
     2461 tataaaattt aaacaaccga ttaatgtttt aaatgtgact ctccttgttg aagatataaa
     2521 attagcaaaa atccttcctt tgttgaaaga acctgaacaa atttttctta tcgaaaaatt
     2581 tatctttgat aaaactgata aagaaattgg aaaggttctg gggattacaa gacagggtgc
     2641 gacaaattta aaacatcgac tttacaaaaa aattcaaaaa atgctggttt aatatctgca
     2701 taataactaa ttttgtatgc ttctttgctc gttctcctcc ttcccaaaga aatagattat
     2761 aagtccatgg gaagaaggta gatttatgaa ttctttaaaa gaagatttca ttttagcaaa
     2821 ggctggaaat gaagaagctg ttgaggcaat tctcaaaaga tttagctctt tgattcataa
     2881 gcaatcctgg agaactggaa aatatgatca agattgttat caagaatgta tgcttgcaat
     2941 atatctagct atctcgaaat tcgaaattaa agaataactc aactaaggga gtctgtgaca
     3001 taagtaaaaa gatagtttca aatttcgaac aataaatttg aaactatctt ctttataatt
     3061 ataaagtatc gattttacag cgttctataa tgattcatag aatctaaaac gaatgatttt
     3121 aattatcaac gttttttaat tcgtttttag cttaaaaata acttttgtca cagtctcact
     3181 cctaaaatta ctttatttac gtatgaagtg ggcaggaggt cgaatccaga actgggctat
     3241 ggtacttcaa cgaggacttc tcctctagaa ttgaaaagca tgcgatttac cttccgtaac
     3301 gacggtagat ttattcaact atgtgtccaa tcaaaatttg tctgatttca aaaatagtca
     3361 agagaatttt ggttgttaaa aactcttatc tatcaacttc cacagtttag ttgacaaacc
     3421 ctgatactaa gttgggctat gatacacttt tcttgactat cagttacgtt accggaagga
     3481 atgaactcta ttgagaaaaa catatgatcg taaatttaaa ctaaagattt cacaggatat
     3541 ccttgagaaa aaagtaacta ccaagaaaat tgctgaagaa tacaatatct ctcgtccaac
     3601 aatttcccga tgggtttcag aatatcgtcg atacgggaaa aatgcttttg ctggacaagg
     3661 aaaaagatta cctgataaag cagatttcta cattcttgaa caggaaaata aacgactaaa
     3721 agaggaagac gagtgttcta cgaacacaaa gatcgatacg gatctccagg gatcactcaa
     3781 aaactttacg atgaaggaat cgaaatcaat aaacgtgttg tagctgttct tatgcgtgaa
     3841 atgaatttat gcacaagagg atttcatcga cgcaaatctt ctcatggtag agaaaaaaca
     3901 atcaaagaaa tggtaaaaaa atgttgaatc gataatttga acagaaccaa attgatgctg
     3961 tttgggtgat ggatattact tacattccat gtaatgacgg tcgcctctat ttgtccacct
     4021 atattgactt agcaactcgt attctacgtt actatatggt agattcacac atgaaaaaag
     4081 agattgtcgt ccgtccatta caaatttatt aaggagaatt gcctacggta attcattccg
     4141 atagaggtag ccagcatcgg tcttttattt atcaagagct gttgaacgac caccacatca
     4201 cacattcagt gagccaaact gggactcctg ttgacaatgc agtaattgaa gcgtttcatc
     4261 gatcaatcaa atgagaatta atcgactcca acaaatataa aagtaaaata gagatgaagg
     4321 tgttaatcca agattattta gaagactact acccaaacaa aagaatccat acaaaattta
     4381 tgatgactcc ttgacaattt gaagcgaaga aaataaggga tctataatat ctagtgttgg
     4441 tgacgccttc tgttgccaat actttttctt ttttctaata aacgcaaaca gacaccttaa
     4501 aatcctttag aataaagtcg acgaaaacca ttcaaaggag tgtttcaaga tgtcctattc
     4561 agagtttacc aaagagttac tagatattct agacctaaat cttatctttc atgaagatgc
     4621 ctttcgcaaa gagcgaatca atgacgaaac atgttttgtt tttgatggca cattgaccta
     4681 tcaaccggag gaatgctttc atcgccacta tcaaaatgaa caaacgatca ttaaataggg
     4741 ctggaaaaaa gtatccatct tattgaatga tgtcagtaac tacaagacca ttctgcggat
     4801 taacaaacag cgcttcaaat gtaaacactg tggaaagact tttttagcag aagattcagt
     4861 tagtgatcgt cgctgctcca ttgctcgcag agtcaaacaa gccattcttg agctattaag
     4921 cgaacctctt tctatgtctt tgatcgcacg tatgaagcac atttctccga ccactgtcat
     4981 tcgaatactt cgcagccttc ggcctaaaac catttctttg agccagctgt taccagaagt
     5041 ggtgtgtttc gatgaattca aatcagtgaa aaatgtctcg ggagccatga gctttgtcat
     5101 gatggacggc aagacccatc aactgattga tattgtggaa aatcggcaac tcaatccttt
     5161 acgcgactat ttcttgcgct accctataaa agtccgtgaa caagtgcgtc tagtggtttc
     5221 agatttctat acaccctatc gcacattggt gaaagagtgt tttccgaatg cccgaatcgt
     5281 cgccgatcgc tttcatatta gccaacacat tggtcgggct tttaccaacc accgaattca
     5341 agtaatgaag acctttaaaa aaggggatcg tcggtcgaag cacttaaaga aatactggaa
     5401 actacttcaa aagaatgcgt gggaattgaa aggtcagcat cgctactggc gtccgtcatt
     5461 tcgagatcat ctgacagaag cagagatagt cgatcggcta ttatcttatg atgattcctt
     5521 aaagcgaggc tacgaagtct accaggattt tctttccgtt atcagaagac aagatgttcc
     5581 tgaattcgtc gcgctcctaa aagaagacta caaggagttg ccagagcact accaaccagt
     5641 gttcacgacc ttcaagaaat accaaacaga aatcaaacga gcgttgcgcg ttccctattc
     5701 caacggccct atagaatgtt tgaataacca tatcaaagta ctaaaacgaa ttgcctatgg
     5761 ctatagaaac tttcaaaact accgagaacg aatcttttta tatcgaggaa aatattttaa
     5821 gaaaactaag aatactaccc aactgactaa agccagaaca acaacacggc tagacaagat
     5881 agctgtctag ccgtgtgagt atctcgatta ttctattgaa tttcttcacc aacactcatt
     5941 gacgaagagc caaaataagt aatctaaccg actgttaact aaaccattgc aggtcccgtt
     6001 aaagatactt gtgcctgttg tcaccgcttc tttttcttgt ccacaagcgc ttaacacact
     6061 taataaagcc cctgctatta ttaataataa tatctttttc ttattaaaaa cctcctcatc
     6121 tatttccaga ttagtaaatg tagtaatctg tgctttcttt tttctcaata ctaataacta
     6181 acttatgggg taaacaaata ctcgtttgac cgggttttga gatccatcca gttctaaccg
     6241 cgatttgatc aggactatta tcttctttaa ctcgaattct tccatcttta atctcaacaa
     6301 tattatattg tcccttcgca ggatgataat tcacgagttt atgattgatc ttatccaaat
     6361 cgaagcgatc catttccttg ccatcaattc gaacgatggc atatttgata gcatcttcag
     6421 ttgtttcatt ctccttttct atagagaata caaataaggg aataaaagag gtaataatca
     6481 aaatgccaat aattatgaca tcgcctatct tgacaccagc ttttcttaaa aatttcatta
     6541 acgatatctg gctaaacata ttagactttt tccatggaaa tggtgaaatt tttcggaaat
     6601 tatctgcatt aagagaatca gttaaacaac cctcaagttg ttccttaata cattgttgat
     6661 ccatcttcat accaataaaa actagtttat taatgcgttc accaaacaac ggatgccagg
     6721 cttccttcaa ttcgggattt ttttgtataa tcatttgaca ctcaatttta ggtaaactag
     6781 ctacccaata atttaatgat tcaatacttt tatagctacc cgatgtttca aagatcgtcg
     6841 aaaaatcact atgttgggca aaccaaagaa acccttttgc tcttgtaata ttgccaggaa
     6901 aatttttatc cgtaagatgg gcaaattttt gtgcgtcaag gagtaaccga tttcgataga
     6961 taaaacttga gataccatat tcttctgttt caggaatatg attttgatag ccaagctcta
     7021 actctttgaa ccagcccact gaataggaag tctcatctat attaaaaaga tttgtattta
     7081 gaattttcga taagtctact tcacctttaa ttgtcctaat tattttggca ttggactgta
     7141 acttagcaat tagaccgtta attttgtaaa tcgtcttggt tgacactaag tcactattat
     7201 ttaaaagtaa tatgttacaa cactcgaatt gatcaattaa caagccacga atatcctgtt
     7261 cttcttcaac tacattacct tcagcttttc tttcctgaat cttttcaccc gtttcgaaat
     7321 tatcccaaaa acgttttaca tccactacta taataatggc accaatctct aatcgttggg
     7381 tcaaatcaat gtctaaaggg tttttagcta atataattgc ttgaataatt ggaattggtt
     7441 tgctgattcc tgaagcttca ataacaattt gattcaagtc agaaaattgc actaattgat
     7501 ctagttcaac aatcaaatct tcgcgtaatg tacaacaaat gcatccattc ggcatcgaaa
     7561 caagtttttc tccagttctt ttaaaacctt tcttgtcgac taatacccca tcaacattta
     7621 cttcccctat gtcatttaca atgacagcaa tttcctttcc gtgatcaccg tacagaatat
     7681 gatttagaag tatcgttttt cctgaaccaa gatagccagt taagattgta ataggtattt
     7741 ttttcatagg gaacctcttt ctataccttg aatttacact ttaagtgcaa cacataaaaa
     7801 tgagtcataa tcgatatcca actagtataa gtttgtcgag aacactccta gaaggaaaat
     7861 aattatgagc cagtatcgtc atcttactat actcgaacga gaaagaatct ttctgcttca
     7921 cgaagaagga ttttctatta gtataattgg caacgacatt cagcatagcc cgtttactat
     7981 tttacatgaa ctgagaagaa acaagaaaaa aactattcac cttccaaagc ggaaaaaaat
     8041 tatgcttatc gaagaaaaaa atgcggacgt tatcacattc taaatgatcc gtgagtttgg
     8101 tcaattgttc gtcatttatt tgtcgatttt cagtggtcgc cagaggaaat ctcgaatcgt
     8161 ttgaagcatg aacatatgga cttttcaatt agttttatca cgatctaacg tgcaatttgc
     8221 aatgatcttt tcgatacaga acccctctca cacggcaata aaggcctgat ccgttcattg
     8281 caccatcgag gaaaaacaag acatacaaaa aatcatgtcg agtctcgtgg aaagatttgc
     8341 cttactcatt cgatccatgg ccgtccaaaa tccgcagatg atagaagtga ttttggtcat
     8401 tggaaagccg atattgtagc tggcaaaact ggtggtactt gtttagtgac tttgacagac
     8461 agaaaatcat gcttcctttt agcaggaaag aaggtcccca gaaagaaagc cgcttatgtg
     8521 agcgagaaaa tgattgaact tttaagcagt attccaaatg aggcggtcaa aacgatcact
     8581 ccagatagag gaaaagaatt ctccaatcat ttcgcagtta cagaagcatt gaataatatg
     8641 cccttttatt ttccagatcc tcatgccctt ggcaacaatg aacaaattaa aatacgaata
     8701 gactcattcg agaatacttg ccaaaatcga aagagatagc tacggtagat aactcaacga
     8761 taaatcagat gattgaaaaa ctgaataaca gacctagaaa gtgtttagat tggagaacac
     8821 cttatgaagt cttctttgat aaagtgttgc gcttgatttg acaattcaag acataataaa
     8881 aagcagatca aatccaaata agggaaaaat gatctactct ggtattatca attaattggc
     8941 caagctatta tgccgttttg ctaaacgtct acttcttcca gtattgacct tgataaaaca
     9001 acaatcagga tcccgtttta atactctatg aactgtgtca atactaacat gtaaagttgc
     9061 agcaatatca gctaatgtac tcccatttaa tctcatttgc tttgcaaatc gataacgccg
     9121 ataagcatca atattcttgg aatccataga caataatact tttcttacca tgtatttcct
     9181 ccaaaaattg aattttaatg agccatcatt tcttgagcaa tttcatgttt agacaatgaa
     9241 gctggataga aagtaggcca attatgcaat tcattataga gtttttcttg actttctcca
     9301 ccaaagaaaa tatggaaatg ctctgcctta cttggtgcaa ttccatgatc actaaattga
     9361 atatatttaa aacgaccagc acctgcatca tcagtttcaa aattaaaacg aacaccacgg
     9421 tttccttttt cataattcaa aattttgtat ccaacatatt tgtatgtgta ttgatgatgt
     9481 tcaccgttga ctacaaaatc aatggtgttg tctttaatgt tgatagtatc aatatctgtc
     9541 ttatagccag tagtataata atctttgtac tcggcagctg tcatgtcttt attgattttt
     9601 gctttgtaat cgaaaacttc atccaaaatt ccatctttta acaacggata aacagattgc
     9661 cattcgcctg tataatcact caattcgcga tccttcacat cggcatcttt aaagtaccca
     9721 ttagcaactg tcttttcatc cttcgcttct ttttctggaa ctacctcttc accagcaacc
     9781 atagttgttt tttctaacgc ttttagattg tcttccatta ctgaaacata atcttcacca
     9841 ttatccattt gttcttttgt aagactttca agaggattca gtacttccaa ttttatccca
     9901 gcttcgttgg ccaaagtttt tgcaatttta tcattagcat tcttttcaaa gtaaatataa
     9961 ttgatcttat tttttttaac atactctttt aattcagcta atcgaccaga agatggctct
    10021 tcttcaggac ttagtccagc aataggaact tgtttcaatc cataatccaa agctaaatat
    10081 ccaaaggctg cgtgttgcgt cacaaaactt ttttgtttcg cttctttaag actagtggta
    10141 tagtcagcat ccaaccgttt cagtttagtc aaatactttt cagcatttgt ctcaaatact
    10201 tttgcttttt tcggatacaa tttaactaac tgatccttga tatttgaaac ttccttaatt
    10261 gccattttgg gtgaaaccca cgtatgagga tctagttcat gatggtgatc ttcttctccg
    10321 tgatcatgat cttcttcttc actacctggc aataaaatca tgtcttttgt tccttctact
    10381 acattaggcc cagctttctt ccaactttct ttagcttttg gtacccatga ttccatgttt
    10441 tcgttatgat atacaaaaac atcagcatca tgagcttttg ccatatcctt agctgagggc
    10501 tcatagtcat gaggttctga tcccgctgga accattaaat ccacgttcgc ttcatcgccg
    10561 acgatgtttt tggtgaaatc atacattgga tagaatgttg ttacaatttc taattttcca
    10621 tctttatcat tcgatacttt tgcatcatta gtgctggagc aagctcccaa taatcctaga
    10681 gctgctatcc caataagtcc tacactgatt ttcttcattc tttttccttc cttaattatg
    10741 ctaatttctt cattttattt cgtttatgtt ctttgataat ttttaatgcc ctttttctag
    10801 ttttaatatt cgggctttta agtcgtcgat atgcactaga tatatccgtt ttctccatta
    10861 gaaattaccc ccaatcacta cttatcatta tttgctttcc gccagtaatt tatcaaaact
    10921 ctgtttgatc ctttcttcat ccaacatttt tccgattaga ataatagtgg tttggtcttt
    10981 ttgtcgagtg gtgtattgaa aattaacctg ttcgtttacc tcttgcatgg caactataaa
    11041 atcctgctct tgtaaagcaa tcaatccttt aaaacgatac aggctccctt ggttggttaa
    11101 aatcagccag tcaatccatt ggagtaaaaa atcttttttt atgtcttgat ctgaagttaa
    11161 gagaattgat ttaaactcat gtacttgatg gtgttgatga tagtatttgc cattatagtg
    11221 attttctgat gaatcacttt caaaaactaa gggatgatta aattttttca aattataaaa
    11281 atcctttttt tgaatcgttt cactatgtgt gaacattcga atatcggata aagggttaat
    11341 acttttgatt tctctctgta atttttttag tgcagataaa ttcaagtttt gttttctaga
    11401 cacaaaaatg cgatctgcca tgacaagttg cttaagggct tgtggttctc tttcaataag
    11461 ttcaaaactg tcactatcaa taacagtcaa taaactatcg atatatactc ttcctcttag
    11521 ttgaggcgct gttaaaagtg tttgaatgat tggttgggga tctgctatac ctgtagtttc
    11581 aatgatgatt tgtgaaattg gttgttgatg ttcttctatt gtttccagta aagaaatcaa
    11641 tattgctaat aaatcactgc gaagactgca acacatacat ccattattta gttgtaacac
    11701 ccgttcctcg ctatgaacta ataatttgtg atcaatccca acttcaccaa actcattttc
    11761 aattataata atttcttccc tggggtaggg tgagacttgc aaggcttgat tgattagcgt
    11821 tgtttttcca gcccctaaaa acccagaaac gattgtaact ggtatgccca ttttgtcacc
    11881 tttttaccac ttagctattt cacttcttta aaaattgccc gtctgcgcaa ttttggcgaa
    11941 tactttttta attctaaccg ttcagggtta ttccgtttat ttttgctggt gagatacaga
    12001 cgttctccag tttcgacaca ttccaaaata atattttgac gcataaatct tcctccttca
    12061 atacttatcg atcttcaaga tatgcttgaa tttgttgtgc atcttcaaaa gttaactcgc
    12121 cagattccaa ttgtgaaact aaattttctt tcatatcaac aggtatctcc caataaccat
    12181 cgatacattg aaataactcc tcgtcatact cttcaaaaat tggatagatt cttaaaaaat
    12241 catcaaatga taagccttgg tcctcatagt aatctttgac taatccattt tcaattgcct
    12301 gttgggtcgg taaacccgtt tcagtaataa ttcctctctc taccatcgaa cgataaaaag
    12361 tgcatttatc tagcgattgc tgggtcgttt caagataata ctttaatcct tcttcaacta
    12421 aaggtgggta aataatttcc attttgattc cctcctaaaa gttatatatt aaactatttt
    12481 gaagctcctt taattacttt ctttccatta tagtaccctt ttaaagatac atgatggcta
    12541 cgtctataat caccattcgc ttcgtcaaat gaaatttctg gttttactac agtttgatga
    12601 gttcttctta accgtttctt cgccttagat gtttttcttg ctggtactgc catgatattt
    12661 cctcctttta ccaacttgct ttttttactc caggaatcaa cccttgatgg gcaagttctc
    12721 taaacttaat acgggacata ccgaactttc gcatataccc tcttggacga ccatccgtct
    12781 gatcacgaag cctcagacga ttcggatttg aatctcttgg caatttagcg agagcggtgc
    12841 gatctcctgc ggcttttaat tcctgacgtt tatctgcata cttttcaatc atcgccattt
    12901 gtttctttgc tttagcaatt ttcgactttt ttgccatata cctttatact ccttttattt
    12961 tgatttagac acctcaaatc gtaatcgtta cgttttgaca ctgatacttt accaacaaga
    13021 gttgctcttg tcaacaataa ataacatgaa ataaaactca tatgaataga taactatttg
    13081 aaaacgaatt tatcggtaca tagagataat caaatcattt aaaagaatat gcagtttagg
    13141 ctctgaaaaa taagaattca cttttattag tccaatcaaa cacaaatacc gttttaagta
    13201 gcttttcctc tacttaaaac ggcaatatga aagaaactat aactttttat tcaatctttt
    13261 atgtttaact ggtttatcgc caatttttta aaactttgat tattccttat attataagaa
    13321 tagacattaa gtgaactaat tcaatcatac tgattctttc gtgtattcaa aattagatgc
    13381 tgtttaataa aattattctt tttgaaaaag aattataact atatcccaaa aattgtaggt
    13441 taatttaaag cctatttcat catcgtgatt gagcttcttt ttcatagacg aatttctcag
    13501 atagtttagt tttctttatg agatttttgt atatttctga ccttcgatac aactcattat
    13561 gagtaccttg atcaactagt tttccttgat ccatcactac aattttattt accttttcaa
    13621 tagacttcaa gcgatgcgat ataattagta ctgtcttatc ttgtattaaa cgatttaaac
    13681 tttcctgaat acgtttttca ttttccgcat ctaaactggc agtaatttca tctaaaatta
    13741 aaattggcgc atttttcaaa aaagcccgtg ctatggatag gcgttgtcgc tctcctccag
    13801 aaagtgaact tccgttttct ccgaccaatg tatgataacc gtcaggtagt ttttcaataa
    13861 agtcttcgca gttagctaaa cgagctgctt ttttcacttc ctcatctgac gcttgactat
    13921 ttccaatacg tatattttcc aaaatacttg tattaaacaa agttacctct tgaaaaacaa
    13981 tagcgatttt gctaaacaaa gaagctggag atacacgttt aatatcatac ccatcaatta
    14041 aaatacagcc ttcatcataa tcatacagtc tagacactaa tttcaagata ctagttttcc
    14101 cggaaccact agcacctact aacgcagtca cttctccttg tttagccgta aaagagatat
    14161 gatctaaaat tggggtattg ttatcatatg agaaagagac atctcgcaac tcaatgtcaa
    14221 aagatttcag aggactatct gatccctctt ggatactggt ttccttcata gctctaattc
    14281 gctgtatttt aggagctaaa taaaatattt caagcactgc ttctttcata gaatcgaagg
    14341 aatcctttat ttttattgcg gctagtaagt aaccaacgac gtagagtata gtcacttcac
    14401 ccgttaacag taagtgaaca cctactagaa tgactactgc taaactaaca tatgagaaaa
    14461 tagaggataa ggccatgact gaaaaagtac ttaattctac ctttaaatga atcctttcac
    14521 tctcttccat ttttttgaaa agtctgtctt gaactttttt agatagatta aagctattaa
    14581 tctcttgctg caattctata gtttcttgaa attcttccga gttttctctc aaagtatcgt
    14641 aatatttagt attggctttc gtttgggatt tctttgataa caagattaag acaaaactaa
    14701 ataacgttgg caaaataaca gctaatccca ttttgacatt accaaccaaa agcatcactg
    14761 aaataaaagg gaaaaacaga gccataccgc cggctttagg tattgcatga ctcatcgcat
    14821 gctcaatacc ttcaacgtca gacatgatag tttgagataa atctgataaa ttatgttttg
    14881 aaaaatagga taatggtaaa tttgacaaat cgtctgcaat ttgcactctt aaatgcgcac
    14941 tttctttata agtactatta tataagtttt catactccct atctaacaac caatacatga
    15001 aaagcaaagt aacaaacgat acggcaaaat aaagccaacg gggctttgta ttttctaaaa
    15061 ctaattcgtc tatcagcatc attataatga atgctggtac catattaatg cagtatacaa
    15121 aaaaactaga aatagaagct ttagttaaag cttctgcgcc ctgatcactt aaagcaaatt
    15181 tgtttttata aaaacttttc attgtcaagc ctccaatcat ttgcattctc ataattagtg
    15241 accaattgtt tatacacttg atgtttagcc attaattcat tatgatttcc acgttctact
    15301 attttccctt ctgacaagac aagaatttca tctacagatt taatgctgga taatcgatga
    15361 gctatcataa taactgtttt ttctttcatc agatgttgaa aagccttttg tagttcatac
    15421 tcattatctg gatcaataga tgcactggct tcatccataa taacaatctt tgcatctttt
    15481 aaaattgctc tggcaatagc tatacgttgt ttttctcctc ctgacagata aactccttta
    15541 ctaccaatta aggtatgctc tctttcggga aatttttcta aaatcgattc gcaaccggct
    15601 aatcttagcg cgtcaaatac tttacttgct ggcgcttgag gatttgctat agaaacgtta
    15661 tcgtaaatac tctgattgaa tagtttcgta tcttgaaaga caaaagcaat tgtgttaata
    15721 attgtgtctt ttgaatagtg atttaaaggc ttaccaccaa ttttaatctg tcccttatcc
    15781 acattgtaga aacctgaaat tagtttagct atcgtactct ttccgcttcc tgattttcct
    15841 actaatgcat aaattctttt ttgttctaaa actaagttga aattttctaa aacacactct
    15901 tctttgtatg aaaagctgac attttcaaat gcaatgtcac aatttttaat ctgtgtctcg
    15961 ttcccatatt ttagttggtc ttgcttcatt tctaaataaa gggagtctag tgtttcgact
    16021 gcatatcttg cttcaaacaa gtgcatactt acatacatta ttctcatgaa agaaacaaaa
    16081 ataattccac ttaaaaaaaa caacatcaac aagtcaacag ctaatatctt gggagtggta
    16141 agagaagtta taaaaaaggt tataggaact atcaaaatgc aaactatgcc aaaaaatagc
    16201 cattgaaaaa gaacaaaagg tgttttccca ctcaaagaat acttatatgc aaacttagca
    16261 taatcgttaa ttgctttatt caaggcttta aatgatgtta ctttagcacc aaatatcttt
    16321 ataacttgaa ttccgcggat atattctacc gtttcagagc ttaacacatc taacgattct
    16381 tgatattttt gaataaaatt tgtattctct gtcattttct ttaacaagaa tacaccaatt
    16441 actaacatgg cgattagaac caatccaatt ttaaagttaa tgaaaaagcc taaggcaact
    16501 gataaaacag gtgttactat ggcttgagca ctatctggaa tcaagtgagc aattgccata
    16561 tgtgttttag ccgcgttgtc atcaattgtc tttcgaatat atcccgaaga atttaaatca
    16621 aaaaaacgaa aacttgcttc tgttaaaccg ctaattccct tttttctcaa attagtctct
    16681 aagcgaaacc caaggatatg agaaaataag ccagaaaaaa tataaaataa agctctaaag
    16741 ttaaccaagc aacagtttga accgcataac tcttagctaa atattcatct cctacaataa
    16801 ttagagactg aagaaatttg taaatactgt aatatccata gactgttaat acgacagaaa
    16861 aatcagaaaa taaagtggct aaccatccgt agtatttcat tttagggaca taactaaata
    16921 attttttgta tactttcatt ttttcctccc ccgcattcaa tttcttctct tattcgttta
    16981 caactgttaa tgctttccgt aatagcgtaa attagagatg tacaaacaga taaaaggtaa
    17041 actacgttct attttttgta tttttttaag acttttcttc caatcaatac accaatcata
    17101 gataaacttg ctacaactaa cactgaaatg attccccata tacctaccgc atagtggatc
    17161 attgcatcca ttgttgcagc agattgtcct ttatctatcc aatcttgacg aaaacgatct
    17221 gtaaagaacc atataggaat ccactgtccg gcagcactgc ctaattgcat tagagcccaa
    17281 gacgcaactt gaggaattgc tcgatcatat ccaaatacag atagaataat atcagctaag
    17341 atcgccatac taatcgaact gataaaccaa ggtaagtacg ccccgcccat taacgtaaaa
    17401 aataacaata aaacaattgt gtaaatagta aaaattcctt ttctagggta gttatagcta
    17461 atataaacaa atatgattcc agataataat ccaaaaatcc cgggggatat gctgtgacca
    17521 aacgccccac caatactact aaccattatt gaaaaaaagt aaattactag ggaaataact
    17581 gtagctaaac caataaaaat ataatctttt gttttcatat atattctcct caattctatt
    17641 tcgttatgtc attaaaaatc tgttctatca aagttgaatc ttctactcta agttttatat
    17701 ctttttttat tccaccattt tttaaataaa taattctatt gcatgtttta taaagaaatt
    17761 ccggatcatg agtagctata actactgcac ccttttcttt taagtcttta ataaggtcag
    17821 caatcctttc catattaata taatccaacc cacttgtagg ttcatcaaat agaaataaat
    17881 tagtttttga caatgaagct atagaaacca ataatctttg tttttctcca ccggataaat
    17941 caaacggtaa ttgatctttc attttataaa gttccatttt ttttaaaatt ttttcttgct
    18001 gatctttact aactatttca ttccccaggg ctaactcgct cctcactgat tctgtgaaaa
    18061 attggtaatc catttcttgc attactaaaa acggcgatga caaatttttt ttattttcga
    18121 ttatctttcc tttggtaggg cgaataatct tcatgagtat tcttagtaag gttgttttcc
    18181 ccacaccgtt tttccctacc aaaccaataa catctccaac tttgacatca aaatttatgt
    18241 cttttagaat attttcataa gtaatttctt caacttttaa tacatctgca cttacctttt
    18301 cgatataggg aacttcaatc gaactattaa aaatgtcaaa tttccttaat gcgttattgt
    18361 tttcagtttt taacaactga atagttgtag tttttacagt tctattttta acaaatagaa
    18421 cttgatcaat taattctttc aaatagtaaa aacgatgttc agctataaca atagtaaatc
    18481 ctttattttt taaatcacaa agtaagtcag caagtttctt agtcgctttg aagtccaaat
    18541 ttgaagacgg ttcatccaat ataattaatt tagagtttag cgataaacta catccaatag
    18601 aaattttctg ccgttcacca cttgatagcg aaaaaatgtt tttatctaat atatttgaaa
    18661 gatctaataa ttcagctaat tcattgattc ttttatccat atgctctttt gtaaatccaa
    18721 agttttccat cgaaaatact aactcactcg ttgtatttgt tgtaaagaat tgtcctctag
    18781 gatcttgact tacgtagcca atattttttg atatactttc ttgtgttaag gattgtagcg
    18841 agttattttc aaataaaatt tccccaacca attttccgtc ataaagtctt ggaataagtc
    18901 cattcattaa ctttaatagt gtggatttcc cactcccaga agaaccagta atcaatgtaa
    18961 gactgccttt ttttattgaa aaattaaggt caacaagcgc ttgttcctta acatccttat
    19021 atgaaaaaga aacattgtta aattcaaaca attctttcac ctcaaatcaa aatacttaag
    19081 aaaaaaatag taacggaaaa caggatgata tagtctaata agctcatgtt tgttttatag
    19141 taagaacttc ttttcacagg agattctatc ccctttgtta ttgcattagc ggtcatttct
    19201 tcagcaatca tagttgcacg tataagaacg ggaactaaca gaaattccaa atattgcaga
    19261 ggctgaaaaa aagacaagcg aatgtttctc atttttaaag attctttaat cattcttatt
    19321 tcagaacgat aagtcggaaa gaatctgatc actactgtaa tacttaacat tatttttttc
    19381 ggtaaatgta ttttttctaa agaggataat aattcattgc tagtatagac actaaccaac
    19441 aaccatgaga tcattacgac cggcaacatt cgaaatacaa tatataagga tgtcgcaaaa
    19501 aatatagtaa aaaactctat cttgatagca tcaataattt tcattactaa aaacagaact
    19561 gaataaatca agatgaaata aatacatgtc tttattttac gaaacatcaa tactaaaact
    19621 gtagcaataa tgaaacaccc cacttgaaat atatttccct ttcctaaaaa aat
//
LOCUS       DAYYDS010000047        19437 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000047 DAYYDS010000000
VERSION     DAYYDS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.047"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..66)
                     /locus_tag="ACHA02_001709"
     CDS             complement(<1..66)
                     /locus_tag="ACHA02_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8670037.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEW"
     gene            339..2810
                     /locus_tag="ACHA02_001710"
     CDS             339..2810
                     /locus_tag="ACHA02_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8670038.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2913..4649)
                     /locus_tag="ACHA02_001711"
     CDS             complement(2913..4649)
                     /locus_tag="ACHA02_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8670039.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4646..5350)
                     /locus_tag="ACHA02_001712"
     CDS             complement(4646..5350)
                     /locus_tag="ACHA02_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8670040.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5481..5984
                     /locus_tag="ACHA02_001713"
     CDS             5481..5984
                     /locus_tag="ACHA02_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8670041.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5944..6282
                     /locus_tag="ACHA02_001714"
     CDS             5944..6282
                     /locus_tag="ACHA02_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8670042.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6303..7721
                     /gene="ffh"
                     /locus_tag="ACHA02_001715"
     CDS             6303..7721
                     /gene="ffh"
                     /locus_tag="ACHA02_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8670043.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7833..8411
                     /locus_tag="ACHA02_001716"
     CDS             7833..8411
                     /locus_tag="ACHA02_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8670044.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8415..8936
                     /locus_tag="ACHA02_001717"
     CDS             8415..8936
                     /locus_tag="ACHA02_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8670045.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9015..9569)
                     /locus_tag="ACHA02_001718"
     CDS             complement(9015..9569)
                     /locus_tag="ACHA02_001718"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8670046.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9822..10097
                     /gene="rpsP"
                     /locus_tag="ACHA02_001719"
     CDS             9822..10097
                     /gene="rpsP"
                     /locus_tag="ACHA02_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8670047.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10109..10360
                     /locus_tag="ACHA02_001720"
     CDS             10109..10360
                     /locus_tag="ACHA02_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8670048.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10485..11276
                     /locus_tag="ACHA02_001721"
     CDS             10485..11276
                     /locus_tag="ACHA02_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670049.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11446..11967
                     /gene="rimM"
                     /locus_tag="ACHA02_001722"
     CDS             11446..11967
                     /gene="rimM"
                     /locus_tag="ACHA02_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8670050.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11957..12718
                     /gene="trmD"
                     /locus_tag="ACHA02_001723"
     CDS             11957..12718
                     /gene="trmD"
                     /locus_tag="ACHA02_001723"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8670051.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12854..13201
                     /gene="rplS"
                     /locus_tag="ACHA02_001724"
     CDS             12854..13201
                     /gene="rplS"
                     /locus_tag="ACHA02_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8670052.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13333..14469)
                     /locus_tag="ACHA02_001725"
     CDS             complement(13333..14469)
                     /locus_tag="ACHA02_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8670053.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14585..15499)
                     /locus_tag="ACHA02_001726"
     CDS             complement(14585..15499)
                     /locus_tag="ACHA02_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8670054.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15590..16018)
                     /locus_tag="ACHA02_001727"
     CDS             complement(15590..16018)
                     /locus_tag="ACHA02_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8670055.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(16023..16397)
                     /locus_tag="ACHA02_001728"
     CDS             complement(16023..16397)
                     /locus_tag="ACHA02_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8670056.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            16683..16868
                     /locus_tag="ACHA02_001729"
     CDS             16683..16868
                     /locus_tag="ACHA02_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670057.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            16880..17128
                     /locus_tag="ACHA02_001730"
     CDS             16880..17128
                     /locus_tag="ACHA02_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670058.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            17159..17338
                     /locus_tag="ACHA02_001731"
     CDS             17159..17338
                     /locus_tag="ACHA02_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670059.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17381..17851)
                     /locus_tag="ACHA02_001732"
     CDS             complement(17381..17851)
                     /locus_tag="ACHA02_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670060.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            17938..18636
                     /locus_tag="ACHA02_001733"
     CDS             17938..18636
                     /locus_tag="ACHA02_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8670061.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18814..19155
                     /locus_tag="ACHA02_001734"
     CDS             18814..19155
                     /locus_tag="ACHA02_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670062.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19148..>19437
                     /locus_tag="ACHA02_001735"
     CDS             19148..>19437
                     /locus_tag="ACHA02_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8670063.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         6399 a         3394 c         3822 g         5822 t
ORIGIN      
        1 ccattcttca tcaaaaaata aatgtttatc tgttaatccg agtaattttc tagtatgatt
       61 atccattgag agactccttt ggtaggatga cgttttggtc gactaaattt taccaaaagt
      121 gagtctcttt ttgagttgaa acgaagaaaa agtgttagta gaaaatcgcc ttcgattttc
      181 caccaacaca aaatattata gaaccctttt tttttgctat atgacaggaa tgaaaggtgt
      241 ttgcaagctt aatgtgagga acactttcca tatctgttct atactggata ggagaagttt
      301 ggaaatatcc agaaaacgaa atcaatcgag gtagccacat gaagaataaa tggataaatc
      361 gaagtaaaaa aaagaaagta acacaaagat catcagataa agtcatccgt ctttttccat
      421 cgtataaaga aggattaacg gcagatcaag tcaaggaacg aatagaaaaa ggtgcagcaa
      481 ataattcagt cgatcccaca ttcaaaacaa atcagcaaat cgtattggaa aacatattta
      541 cgtatttcaa tttgatcttt cttatcttag ccattttgct ttgtttagta gaatcctata
      601 agaacttgac ttttttacca gttatcatag ccaatacagg aatcgccatt tttcaggaaa
      661 ttcgatccaa aaagatcttg gacgaactaa atgttttaaa tacagcaaaa gtcaatgtca
      721 tacgagacgg gatagaacaa acaatagata tagaagacct tgtaatagat gacattgtta
      781 ttttagagac tggatatcaa attccagcag atgcagaggt cgtggaaggg aaactgcaag
      841 ttaatgaggc tttattgaca ggcgaagcag atgaaataac caaagaaata ggtgatccat
      901 taatgtccgg cagtttcatt gtttccggaa aagcttacgc ccgattagac aaagttggag
      961 cagattcgta tatttcccag ctgacagtca aagccaagac aatgggagaa ggagaacagt
     1021 cggagatgat tgcttctctt aatcaattga tcaaatggat tgggatcatc attattccta
     1081 tcggtatcct gcttttttct caaagttact tttttaatgg aaacacgatc aaagaaagtg
     1141 tcgttgcaat ggaagcagca ttgatcggta tgatacctga aggactgtac ttgctgacga
     1201 cgattgcatt agcattaagt gcgactaaac tagcaaagca aagagttctt ttacacaata
     1261 tgaaaagcat tgagacattg gcaagggtaa atgtcctttg tgtagataaa acaggaacga
     1321 tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc taaaaaacaa gaaataatca
     1381 gtcagtcaga tcaattggaa gcatgcattg ctgactatgc aaaagcgatg gcaggagaca
     1441 atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac cactgatcaa tcctatcacc
     1501 atgttattcc tttttcttct gtccaaaaat tcagcagtgt agcgttggca gacaaggtgt
     1561 atgttttagg agcgcccgaa atggttttgc gcgatcaatt gccggaatat gcggaagagt
     1621 ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt tggtgtgtac gatgggattc
     1681 ttgaagaacc agtattgaca gaagcagtca caccattagg ctatattctg attgctaatc
     1741 ctatcagaaa agaagcaaga gccacatttg attacttcaa aaaacagcaa gtagccatca
     1801 aagtgatttc tggagataat ccgctcaccg tatccaatgt agctgtccaa gcagggattt
     1861 cacatgctga tcagtacgtt gatgcatcta aattatcaga agaagaatat gaagcagctg
     1921 tcgaaaaata tacagttttt ggacgcgtca agccagaaca aaagaaaatc ttcgttcaat
     1981 tgataaagcg taacaataca gtagctatga caggtgatgg cgtgaatgat attctagcca
     2041 tgaaagaagc agactgcagt atagcgatgg cttccggaaa tgaagccgcc atgcaggcct
     2101 cacaagtggt cttacttgat tctgactttt cacgcatgcc agaagttgtt gcagaaggac
     2161 ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt tttagtgaag aatattttct
     2221 cctttttgct ctcactgttt tcagtgatct ttgctctgac ctatccttta gaaccatcac
     2281 aaatcacatt gattagtttg tttactatcg gacttccatc tttcttgctt gctctagaag
     2341 aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt gatggaaaaa gctgtgccag
     2401 gtggactaac agatatgatc gtagtcggtg cgttggtgat ctgcggggtg actttgaatc
     2461 taaataaaac agatgtttct acagcatcta ccatgctttt gattgcagta gggttcctcg
     2521 ttctctataa aatctgttca ccgctgaata aattcagaag tcagatcatt cttttctgtg
     2581 ctagcggtaa ctttttttcg gtcatctttt tgcataagct gttttcaatc accggtatct
     2641 cagctgtatc acttttgctg ttgagcattc tatttttctc ggcagattcg atttttaggc
     2701 atttgaccac attggtagaa tatttgtttg aacgtaaaaa tgaaaaagag caaaagccgt
     2761 ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg agaaaattga cgaaaagtat
     2821 ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt tcgaagcagg atgagtcatc
     2881 caactttaaa acgcacgagc agttgctctt ttttacattt tcggaatggt aacggtgaaa
     2941 gtcgaacctg ttccaagatg actgtctaca gtaatcgtac cattaaggag ttgcacgtag
     3001 tcttttacaa tcgctaaccc taatccggtc ccgcctgaat gtcggcttcg cgccttgtct
     3061 acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa tgtcgatacc tataccaaag
     3121 tcttggacag aaaacgatag atcatctgtt gcctgatagc ggataataat ctcgctgtcg
     3181 ctttttgaat attggacagc attttcaatt agatttttga taattggata aaggatatct
     3241 gttttagtag taaatggttc gttctttcca atcaatcgaa tcgtcaaccg tttcttttcg
     3301 atgattggct gataattttg aacgatttgc tggaaataag gttctgtata aattgtctgg
     3361 atctcgtaag aattctcgcc atcttttgac agctggatga tttcccgaat cagtttatct
     3421 aaccggatcg catctttttg catgatctca agaaaagaag tcagagtttg cgggtcatct
     3481 tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg aagtcacagg agtttttaat
     3541 tcgtgagaga cgttgccaac gaaatccttt tgtagctttt ctaaacgtcg cactttcgtt
     3601 aagtcatacg ccacccctag aatctgtccg gtaccttctg aattagtaaa gtagcgcaaa
     3661 ctcaagtcta atgtctgccg accttctgta agggtgatct cctgatggat cagcggatgc
     3721 tcaggagtca cttgatgaat cagctgaatg agttttggct cttgaatgac ttctgtgtac
     3781 cgctgttcag gttgatagct taccacgttt aaatgtagct gcatctttgg attaagcagt
     3841 agtaaacgag aatcagcatc atcaatcagg aatacgccaa tcatcagttc gttcagtaac
     3901 gtatataatt gctcttctgt ggtagtacac gcccgataca tcttactcat ttgttcgctc
     3961 aacgcattaa tcgtctgata caattcttcc cattgatcgg gtgtttgcat gattacttcc
     4021 gcctgattag gattttgcac catttttttt agtacgggca gcacggtttt caatggctgg
     4081 tttttttgat agatcagata atagatcatt cctgcaagaa caaggaagaa agacagaaag
     4141 aagaaaaaga cccaacggcg aaaactctca gttctcggta aaaaaccgct tgtcggttct
     4201 gcgatacgta aaatggcttc taactggcca ctttttttga ctggtaatgc gacatataaa
     4261 agatcctcat tcaaagtagt acttttgcgc aaagcgctac ctaggttccc accattcaat
     4321 acggctttga tttcgggacg ggagtttcgc tgttcattca atgtttcatt gtttgtgtca
     4381 aataagatct ttccagtagc atccattaaa gtaatccgtt cgttcgactg atggacgaat
     4441 tcttccaaag cagttttgtt ttgcgctgct tggaggtttt cgacatctaa ttgccggatc
     4501 agcagttgtg ctttttttgt caggtagctt tcttgctgtt ccacgatctg ctgttgaaaa
     4561 taatgggaaa tcaattgcca gccaccaaaa aacaaactaa ctaaaagaat aagaaagaag
     4621 agacgttcat aaattttttt ccatttcatc gtttgggttc ctgaaactta tagccaaatc
     4681 ctctaatagt caccaagtac tttgggtgtt ttggatctcg ctcgattttt tctcgcaaat
     4741 gactaacgtg tacatctacg attcggcttt gtcctgcgaa gtcaaaattc caaatgcggt
     4801 ctagcaatgt gtctcggtcg atcacacggt cttttcgttt catgaaataa acaagtaatt
     4861 caaattcttt cggtgttagt tcaatcagat ggtcgtctac ggtcacttga taattggtca
     4921 aatcagcagt gatttgtcca atcgatagat attcaggctc agcttcatct tttgattgct
     4981 tacgaggttc aattcgtctg aatattgctt tcatacgggc taatacttcg cgaggactaa
     5041 atggtttggt caaatagtcg tcagcaccta tttctaggcc aatgatccga tccacttggt
     5101 catctttagc cgtcagcatc aaaatcggtg tttcattttt ttcttgacgc agccgctgag
     5161 tgatctccat gccatccatc gttggaagca tcacatctaa aatgatgaaa tcaaatgatt
     5221 gttctaatgc taattctaat ccttcgcctc catctgtggc actggttacc ttgtagcctt
     5281 ctttttccaa attgaacgtc aagagcgtta caatagaagg ttcatcatca acaactagta
     5341 tttttttcat tcaaatctcc tttaaatagg tttcattctg tttatatttt agcatagaat
     5401 cagtacaaag ggctatttta caaaagaaat aaatggtata ataggaaggc actttgctga
     5461 atagaaagga agagcagtca atgattggaa tcagtgcatg tttaggcggt ctggcctgtc
     5521 gctatgatgg acagaaaaaa gaagtaccag aattgatgga actagttgaa actggagaag
     5581 ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat tcctagagat cccgctgaaa
     5641 tcattggtgg aaatggattc gatgtgtgga aagatcaagc aaaagtatgg acgacaagcg
     5701 gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa agcctatcaa aaattgcagg
     5761 aaaaacacat tgatctagtc atcatgaagg aaaaaagccc ttcatgcgga acccattcga
     5821 tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc cggagtggct actgcttatt
     5881 ttattcagca aaaaatgact gtgctctctg atgaagaatg gctgaaaact agaggtgagc
     5941 agaatggaaa tcgaaaagac caatcgaatg aacgcgctct ttgaatttta ttcgacctta
     6001 ttgacagaaa aacaaatgaa ctacatggaa ttatattatg cagatgattt ttcattaggt
     6061 gagatcgcgg aagaatacga agtaagccga caagcagtat atgacaatat caagcggaca
     6121 agcaagatct tggaggatta tgaaaaaaaa ctccatcttt tttctaatta tattgttcga
     6181 gaacaggtat tggaaaatat gactgcatat attacagata agtacccgga agataaaaaa
     6241 ctacttgaat atgtacagca aattcaagca atcgaagaat aaataaaagg aatgaagaaa
     6301 atatggcatt tgaaagctta actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa
     6361 aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac
     6421 tagaagccga tgttaacttg caagtagtca aagattttac taagcgcgta cgcgaacgtg
     6481 cggtaggggc agaagtgctg gaaagtttat cacctgcaca acaaatcgta aaaatcgttg
     6541 atgaagaatt gacgatcaca cttggttctg aaacagcaga gttaaataaa tcaccaaaaa
     6601 taccaactgt tatcatgatg gctggtcttc aaggggcagg taaaacgacc tttactggta
     6661 aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg
     6721 tatatcgacc agctgcgatc gaccaattga aagtattagg tcaacagcta gacgttcctg
     6781 tatttgatat gggaactgaa gtcagcccgg tagagatcgt ccgtcaaggg atggagttag
     6841 caaaagaaaa gaaaaacgac tatgtattga tcgatacggc aggacgtctg catatcgatg
     6901 aaacattgat ggatgagttg aaacaaatca aagaattgac gcaaccaaat gagattttgc
     6961 ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc
     7021 aattaggtat tacaggggtc gtcatcacta aacttgatgg ggatactcga ggcggggcag
     7081 cactatcgat ccgctcagtg acaggcgcac caatcaaatt catcggttct ggtgaaaaat
     7141 tgactgactt agaagtattc cacccagatc gtatggctag ccgtatcctt ggtatggggg
     7201 atatgctgac attgattgaa aaagcgcagc aagactacga cgagaaaaaa gcggaagaac
     7261 ttgcacagaa aatgcgggaa aattcattcg atttcaatga ctttattgaa cagcttgatc
     7321 aagtgatggg tatgggccca ctggaagatc taatcaaaat gatcccagga atgaatcaag
     7381 tccctggaat cgaaaacgtc aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg
     7441 tttattctat gacgccagca gaaagagcaa atcctgatct gttgaaccca agccgtcgtc
     7501 gccgaattgc tgccggttct ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat
     7561 tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg ggatatgaac atcccaggta
     7621 tggaccaaat gtttggttca ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa
     7681 tgatcaagaa aaataagaag aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg
     7741 gtacagactg aaaatgtcta ctgccggagc ttttctatca gcaattttta ttctttacta
     7801 atacaattac tctaaacgaa gaggtggcac agatgagatg tccgtggagt gaacaaacag
     7861 aaagtatgcg gcaatatcat gatactgtct ggggtgttcc tgaatatgat gaccaaaagc
     7921 tgttccgaaa attgatgctt gatattaatc aagccggatt gagctggcag acgattttaa
     7981 ataaaagtga agcattcgat gaagcatatg atcgattcga tatcaaaaaa gtcgcaaatt
     8041 atggagaaac aaaaatcagt gaactaatgg aaaacaaagg aattattcgt aaccgaagaa
     8101 aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa aattcaagaa gaaatcggca
     8161 gtttttcaga gtatctttgg tcgttttcta atggaaaaat cgttgattct tcttatcatt
     8221 cacaagaaga agtcccaaca acaagtcctc tttcagatga attagcaaaa gatctaaaga
     8281 aaagaggatt taaatttatc ggaagcacaa caatctatgc ttttttagaa gctgtcggga
     8341 tcatcaatga tcaccttgac agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa
     8401 agggcgaata aagcatgcga aaaatcatat tttatggtgc tataagttta gatggctatt
     8461 tggcaacgaa aacggatgat cttcaatggc tttttgacac tccaacaggt aagcaaacta
     8521 cttatgaagc tttttatgaa acaatcgata cgaccatcat gggcagaaaa acttatcagg
     8581 aagcaaaaaa atatttggag accgaagcaa tttatccaga gaaaaagaat ttcgtttttt
     8641 ccacccacgc acaattacaa ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt
     8701 ttctaaaaaa tctacaaatg acagaaggaa aagatatctg ggttgtgggt ggcggtcgat
     8761 tgatcaaacc gctattggaa catcaaatga ttgatgaatg gtatatccag attacccctg
     8821 tattattagg ggacgggatt cctttattcc aaaaaggaac atatgaagcg cgttttgaaa
     8881 tgctagacac gacacgattc ggtgaattta tcgaattgca ttatgtacga aagtagaagt
     8941 aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg tacatagttg ccttttttat
     9001 taccgttatg ctttttactc tgttaatttg ttagaaaaat ctagatacgc atctaaaatc
     9061 gtttggaaga aacttaatgt tccttcaacg atattaccat tttcatcaat tagattcgca
     9121 acgtttccga tatatgcttc cggttgctgc aaggttggca cattcaaaaa gacaagtgat
     9181 tggcgtagat ggtggtttgc gccaaagccg ccgatcccac ctggtgatgc agtaacgact
     9241 aaacctggtt tatgatccca aacactgtgt ccataaggtc ttgagcctac atctaaagca
     9301 tttttcaata ctgctggcac agaacggttg tattcaggag taacgaaaac gacaccgtct
     9361 agtcccctca cttcctcacg aaaacgagtc cattcagcag gagcctgttc tggagtttcg
     9421 agatcctcat tgtaaaatgg cagatcatcg atttttacaa atacactttc gtacccttca
     9481 ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt aagaatcttt tcttaaacta
     9541 ccaataaaaa atccaatttt ttttgtcata cgatttttcc tccttcaaac tatctataat
     9601 aatcttacaa aagataagta actggagcaa ataaaaacac ttactgacaa aagctacata
     9661 gaagatagat tacttatttt taacaaaaaa gaagtctaaa atcttctatt ttctaaaaaa
     9721 aatcgaattt aatcaagctg taaagaaaaa aagctttaca gggtaaaaaa aaactgttac
     9781 actacatctt gtgaataaaa caatggaggt ggaaataaat aatggcagtt aaaatccgtt
     9841 taaaacgtat gggttctaaa aagagtccgt tttaccgtat cgtcgtagct gattcacgtt
     9901 ctcctcgtga tggacgtttc atcgaaactg taggtactta caatcctttg aaagaccctg
     9961 cagaagtagt tttaaaagaa gacttagttc ttgactggtt atcaaaaggt gcacaacctt
    10021 ctgacactgt acgtaacatc ctttcaaaag aaggcgttat gcaaaaacac cacgaagcta
    10081 aattctcaaa gaaataaggt gaccggatat gaaagattta agcgacttag tcttaacaat
    10141 cgttcgtccg ctagtcactt atcctgatca agtcaagtta gacgtcgtag agtcgaaaga
    10201 cttttacgag tacaatctaa cagtggctcc tgaagatatt ggccgtatca ttggaaaaca
    10261 agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt gttcggatca atgcaccgaa
    10321 aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa ataaacgtgc tgtactcgaa
    10381 ttttcgagtg cagctttttt gtttttttta ttaattgaag aaatgattat ttggcaaaac
    10441 aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca gccaatgaga aagaaaatgt
    10501 tatttattcc atttgctcta ttgttaagcg cttgtagttc ggttactcaa gaatcagaca
    10561 atacacatct atcaagcagt atatctgaaa ctgttaccag tccagaaaaa agtagtacca
    10621 aaacggcttc caccactgaa caaacaatgg ctacctcaaa taatgagaaa aagactgctt
    10681 tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc tctagatgta ccagtctctt
    10741 caggctatct caatatcgca gcgacacata caaagcaagg ttattctatt ttatattaca
    10801 gaacagatcg tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa
    10861 cctatctata tcaatatggg tttgcttcta gtcaagaaac aatccaagtt ttgcagccat
    10921 ttgagatcga cacaaatggt cagcaagtgg acttaggttc tcgaatcaca gggtatcagc
    10981 aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg aaactggcgt attcgaatcc
    11041 gtgggaataa tatagaaggc caagatccct tactattagc taaagaaatc gtagcttatt
    11101 tggaagaaaa cagtctacca gcacctgaac aatttggtaa aattactgtt gatatgggtg
    11161 atacgacgaa tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca
    11221 ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc
    11281 agcgcgctga tctgagccca tcatttaact ccaagtcaca ggaagtaatc aaaaatagca
    11341 cgccgcattt ttgcataatt ttggcttaat accttaaaag aaaactagaa ttgcctcttc
    11401 acacatgcta aaataaaaca gacaaaaaag aaatggggat ataagttgac tgaatacttg
    11461 aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca
    11521 acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc taacactatt ccaagacgga
    11581 aaagcacctg tggaattaac agtcaaaagt catcgcaaac ataaaaattt tgacttattg
    11641 agttttgaag gacatccttc tatcaatgat gtggaaaaat atcgggatgg catattaaaa
    11701 gtgtcaaaag acaactctgt agatctagct gaaaatgaat tctattacca tgagatcatc
    11761 ggtctgcatg tattagaaga tggaactgag ttaggtaaag taaaagaaat cttgtcacca
    11821 ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat
    11881 atcgcttctg tcgtcaaaga agtcgattta gaagctggag tggtccgtgt agaaattcca
    11941 gaaggactga ttgacgatga agattgatgt attgacttta tttccaagaa tgtttgaagg
    12001 tcctatgggg gaatccatca tcggtaaggc tgtagataaa ggattattag atatcaatat
    12061 ctctaacttc cgcgattatt cagataacaa acatcagaca gtagatgatt atccatacgg
    12121 cggtggagca ggtatgctgc tcaaagtcca gcccatctat gataacatcc aagccattga
    12181 aaaagctgca ccggatgtca aaaaaagagt aattcttctt gacccagctg gtaaacgatt
    12241 cgatcagaaa atggcagaag aattttcaca agaagaacac ttagtattca tttgtggtca
    12301 ttatgaggga tatgatgaac gtatccgcac attagtgaca gatgaagttt ccttaggaga
    12361 ttatgtcctt actgggggag aattaggtgc gatggtcatg atcgatgcaa cagttcggct
    12421 tttgccagat gtgttaggga atcagacttc agctcaaacg gattcttatt caaccggttt
    12481 attggaacat ccccaatata ctcgcccggc agaatataaa gggatgaaag taccagaagt
    12541 attgacaaat ggaaatcata aattgattga agaatggcaa ttaaaagaat cactaagaag
    12601 gacctatcaa cgtcggcctg atcttttgga aacacttgaa tggacacctc aaatgacgaa
    12661 atttttagaa gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag ccaagtgact
    12721 ttttctaaaa tcttctttac agtaggttga taatatgtta gtattttatg gtgagtgcaa
    12781 aagcacttaa ctattacgat attccgctgt gagaaaagct cataagaata tttggaataa
    12841 ggagaagaaa accatgaatc cattaatcga agaattaaca aaagaacaat tacgttctga
    12901 catcccagcg ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac
    12961 tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag
    13021 cgaaacttat acagtacgta aagtttctaa cggagtgggc gttgaacgta cattcccatt
    13081 gcatacacca cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa
    13141 attgtactac ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa tccgtcgtta
    13201 atcatcgtta tcagactgag aaagaaccct tggtattcaa gggttctttt tttgttctct
    13261 gtttttttat attaacgatt tctatttggg acatttttga ttaataaaaa ggggcaaaaa
    13321 aggggcaaca agttacagtt tttctatatc ttctttcaat ttttctttta ctccctttgt
    13381 cacatgcaaa taaatctttt cagtgatatc actattttcg tgcccaacgc gatcctgaat
    13441 agcatacaga ggagttccta gttccgctaa ttttgaaata tgggtatgtc taaaaatatg
    13501 tgaactaagt tttttatcaa tcttcatgtc agctttatga tttctcaaat aggtgttaat
    13561 tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat agaaattgac cattcggatt
    13621 taattctaga agctcattgt aaatagctat cgcttttttt ggtaaatcaa tttctcgcat
    13681 tccagcagca gtttttgtag aatcagattt tttcatatca gctattgaac gttctctata
    13741 catcatcgtc ccatttataa ctactgatgc attattattg gtaatgtgta catcgtcttt
    13801 acttaatgcg attgcttctc caggcctcat tccagtcaaa tatagccact ggaaaaggag
    13861 agaatatctt ttattgtgtg aagttgtgaa atttactagt cttttatatt catcatcttc
    13921 taaaaactta tctttaattt ttatcgtttt tgactctcgt ttataatcaa taactacttc
    13981 gttgatagga tttttttcta catagccttt tttcatagcg tatgaaaaaa gaagattcaa
    14041 tttggattta attacactga cgtacttatt tgacaagtca tctttatata tcatatcttc
    14101 aaaaatatta tttaaatcta ctgtattaat accagaaacg atgtaagtct caggtatctt
    14161 tttctttata gtgtttaaga tattattcgt aggataatat gtagactcct taacttgtct
    14221 tttgtaaata actaaccatt cttcgacaag ctcatgaaat gttagatcag gcttctgaag
    14281 tgttttttcg tttaatttta tatcaatttt tttatttaac tctagcaatg ccactttctg
    14341 agtttctcga gatttatttt tataagtaat gctcactttt tttctttttc tagtttttgg
    14401 atcaatatat cgctcattat atttatatac tttttttccg tttttatcag ttttggtttc
    14461 aatccacatt gttttcatct cctatttgtt gctataatag gaatggataa gtaagccaat
    14521 tatagcaggt ttatttttca tcacgtccac aaactttggc gagggagggg gcgtgttttt
    14581 ttgtttagta gctacctttc catgaagtat ctgcgaagaa cacttcaaat gttgttccat
    14641 catttttcac ttcaaaatat gctgttcctt gtgcggattt acccggttgg atggtttccg
    14701 aaaagtaatc tctactagct aaatcagatt taatatctga tgaatcataa aattctaaat
    14761 aacttgaact tgcatcaaaa ggtgtagtcc ctgtgttttc tacaagaaaa tctacctttg
    14821 cgtaaaatag gccttctggt ttatagaaat catcaccgta atcttttgat acagactgga
    14881 cagttacgtt aatagagtta ccattttcag aagtaaaagg aactgcttct cctatactat
    14941 gtgcaactga tgccgtctgg ggttctgaag acgaagttgt agttgtagtt gtagtagagc
    15001 ttgaatcttt ggattttcca tctttatcta agaaagttcg ttgattataa tatacaacct
    15061 gttcagtttt tggactaacc caaattaaca ttgtttcatc atcagaatca gttgaataag
    15121 tatattggag catctctaat ttttttatat cttttgcact atttccatgt tcaattaatt
    15181 cagagtgctc tttataataa tctccttgtg catcctttga aatagataca attaattcat
    15241 cccgtgtgat agcattgtct aattctttgt aaacaaaatc atttgtccat ttacgtaacg
    15301 gtgaaccaag tgtctgataa acttgagtta aatcatcacc aacttttatt ttatgaacgt
    15361 ctgatgaagt tagttttttt gtttcatatt cagatgttga tgaaacagta ttttctttag
    15421 tgctagcaga attaggacta gtggagcatc cgcttaatac caagcctaaa aaaagcaacc
    15481 caaaaacaat ttttttcatt tttaattcct cacttctatg atatgatttt tttgtaggat
    15541 cttagaaata aggtttttag tccgtgttgc agcacggact ttttttacat tatataagaa
    15601 agcgataagc gctttctgga agtccgtaaa gattctttaa ttcctcaatt cgtttaggat
    15661 attgatcatt gtcttctttg tatagagaaa caatgagatt agcagcaaag caattagctt
    15721 cgctttcaga tttgcttcta gatgttcttg ttgatacata gtaactggat aagccacgat
    15781 gaaaaatagc gtgacctaat tcgtgagcgc aaatgtagaa tctttcctca gagtctctca
    15841 gttcatcatt taagaagatt attgcacgac ctctaatttc ttgaaactgt cctttgggat
    15901 tctcgataaa aggaacgtat tgaattttaa tgcccatctt ttcacaaata taaaaaggat
    15961 tagcggactg gtatttccgc ttcaactcct cgactaaatt aatcgtatcc atctccataa
    16021 gctcacatct ttttgccttt ttctttgtct tctttcacaa tatcccagaa ggttccaata
    16081 aggatatctt ttacgcgttg gacctgttca ggcgtcagtg tttctccgcc ataagacata
    16141 ttgacatttg aatctagtaa tttatcaagt tcaactactt catcttttgt tgcccagtca
    16201 ggaacttggt ttctaccgag taaataatca gtagttacat taaaataatc tgctacaagt
    16261 tgtagtcttt tagtactagg agttctattt ttccattgat aaatcgtgtt tttaggtata
    16321 tttaattctt cttccaattg agttatactt atgcctcttt tatgagctaa ttcctttatt
    16381 ctatctagta aattcatttt cgcacctcac aagctacgaa aacactaata aaaaaattag
    16441 ttaaaggtgt tgacaactaa taaaagtgtt agtatactgt tttcgtaagc taaaatatta
    16501 gctaaaaaga cttgataact aataaacact tcacggtcgg caaactctga aatgtaaatt
    16561 actaggcatt tctgtgtctt atttagctat gtctatatac taataaaaat attagttatt
    16621 gtcaacgaat attagctaat tttttagctt acagattatt tttttagaaa ggagctattt
    16681 ttatgtctga gaatttagac ttaaaaattc gagcggagat gagaaaaaga agaatgactt
    16741 tcaaagaact agctgcgctt atcggtattt caggagctta tttatcagat attctaaacg
    16801 gcaatcgtga tggaaagaaa gcacaacagc atatcgaaac agtgaaaaaa atattggaca
    16861 tccgataggg gggtaggcga tgacaaaact aaaaaaacaa gattttgtaa aaaaatacaa
    16921 ttattctcca tctacttatc aacgtcgaat gtcggaacta aaaaatacag caattttctc
    16981 agcggcgtat gaacgggtca caggacaaga agtttggatc aatacagaat tatacgataa
    17041 atttttgtct ttcaaatcct ataacaggtt acgcacaaga aaggtaacac ctaaagaatt
    17101 tatcgagaag catttagttg atttataaaa aacagaacat tttgagaggt gaaggttaat
    17161 gggtaaattc aacagagcat tggtattcag cgcaccgcta atcatctacg ctttaggact
    17221 ttggggaagc aggcaagcgt tgataggaac gattgtttac atggtttgga tttttatggg
    17281 gcttgatgaa gctgaagctg agtacagagc gaaaaagcca accgaggggg ctgactaaaa
    17341 atgaatagaa aagagaaact agaatgtata ttactattac tcagtttaat tctttcacta
    17401 atttctctat tgggaagttt gtatttttga tatcaacaat tttgtggatt tgggttctat
    17461 ttagagtaaa tatcattttt gtttggtgat taaggagttt attaaaatta attttcccta
    17521 cttcaaatgc aaggtaacaa gatatagcac ttttaggagg aatatttagc ggtacagctt
    17581 cagagtatat tctattatca tcgactgatc ctcctttagc aattaatttc ttaaatggag
    17641 tagctgacca tctgcggttt aagttagcgg atgaaagttc taaattaact aacatagctg
    17701 gttctgttga atagttagaa atgattactt ttgtataaat catatctgca gcgaagtaag
    17761 aagcattaag ttcaacttct agttgagggc gcattccttt tatttttatc cctgttaaaa
    17821 acgtactcaa tataaaactt attattgaca tccattgaaa aattgttaag tgaaaaaaat
    17881 tcaaaataaa caccaccagt ttttaactaa attataccaa aaaggagaga agaaataatg
    17941 caagaattag taattttgaa aaataaagaa gctgtgacta cgagcttaca agtggcagaa
    18001 agcttcgaga aaaaacatca acatgtttta agagatattg atgcactaaa aaaagatgtg
    18061 tccaattttg gacagatgtt tgtagaaggt aatgaaccag attcatatgg cagaaatcga
    18121 cgagttttct tcattagtag agatggattt ttcttgctgg ctatggggtt tacaggaaag
    18181 aaagctatct acttcaaaca aaaatacatt gaagcattca acgaaatgga agatgttatt
    18241 cgcaagaata ctgttcctca aacaattgaa gatatgatga tctatcaatt agaagaaatg
    18301 aaagatgtta aaaaagatgt ttccatgctt aaagatacta tgcgaattag cggacaacaa
    18361 gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg gagttctagg gggaaaagaa
    18421 agccgagctt atgaagaaat cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa
    18481 cgtacctttt caatcccaag atatggcgag ttacctcgta agagattcga tgatgctgtt
    18541 tcatttattg aaatgtggtt gccagaaact gcgatccgta tggaaattga tcaactgaac
    18601 agacaacaga gactttttgg tgatgacaat gaatagagct gaagcgctaa gaatagggac
    18661 ggcaattgct aattgctggt ggaaatacta taaaccaatc atcctaagcc aacaacatat
    18721 tgacaagcaa aaagcatggc aacaaataaa aaagtgactc cgccggcaag caaagagtca
    18781 caaagaaaac acatcataag gagattttag catatggaaa aagaactttc cactctagat
    18841 caatatttga ttgatcctga ttggggcaag ccgaaaattg aggaaacaag tggtcgaaaa
    18901 atcagacgaa atcttttgac gaatgaagaa ctagcttggg atcaagatga tttaggcaac
    18961 catgtaacta tttgggatca tgtttatctt atccatctat cgaagcattc gaataaacct
    19021 gaatatattt acgtcatcga agatggcttg attgatgcgc tagaagagta cgacagagat
    19081 aacttgattg atatctctta ttacggacca ggtaagaaat acattgctga aatggaggca
    19141 gaatttgatg agtgaaatca aagggacaac gaactttgaa aaacttttta gtcgtaagtt
    19201 aaataaaatt ctcaagaaaa aaggaaattt tgattattta tcttgggctc acgcgtggga
    19261 gattatgaaa aagaatgatc cacaggcaac ggtaactatt aatgagtaca aacactacag
    19321 ggttgtttct ggaactcatc aagactttct tgttgaggaa tataaacctt ttcttatgga
    19381 tgaaactggg acttatgtat ctgtctcagt aacggttaaa ggacacacgg aaaccga
//
LOCUS       DAYYDS010000048        19200 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000048 DAYYDS010000000
VERSION     DAYYDS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.048"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..2543
                     /locus_tag="ACHA02_001736"
     CDS             102..2543
                     /locus_tag="ACHA02_001736"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8670064.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="ACHA02_001737"
     CDS             complement(2873..3577)
                     /locus_tag="ACHA02_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670065.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="ACHA02_001738"
     CDS             complement(3764..4009)
                     /locus_tag="ACHA02_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670066.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="ACHA02_001739"
     CDS             complement(4026..4877)
                     /locus_tag="ACHA02_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8670067.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="ACHA02_001740"
     CDS             5043..6047
                     /locus_tag="ACHA02_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8670068.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6288..6644)
                     /locus_tag="ACHA02_001741"
     CDS             complement(6288..6644)
                     /locus_tag="ACHA02_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8670069.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7012..8199
                     /gene="tuf"
                     /locus_tag="ACHA02_001742"
     CDS             7012..8199
                     /gene="tuf"
                     /locus_tag="ACHA02_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8670070.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="ACHA02_001743"
     CDS             complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="ACHA02_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8670071.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..11765)
                     /locus_tag="ACHA02_001744"
     CDS             complement(11004..11765)
                     /locus_tag="ACHA02_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8670072.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11932..12564
                     /locus_tag="ACHA02_001745"
                     /pseudo
     CDS             11932..12564
                     /locus_tag="ACHA02_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            12623..13483
                     /locus_tag="ACHA02_001746"
     CDS             12623..13483
                     /locus_tag="ACHA02_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8670073.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13668..15983
                     /locus_tag="ACHA02_001747"
     CDS             13668..15983
                     /locus_tag="ACHA02_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8670074.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16165..18006
                     /gene="dnaG"
                     /locus_tag="ACHA02_001748"
     CDS             16165..18006
                     /gene="dnaG"
                     /locus_tag="ACHA02_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8670075.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18030..19139
                     /gene="rpoD"
                     /locus_tag="ACHA02_001749"
     CDS             18030..19139
                     /gene="rpoD"
                     /locus_tag="ACHA02_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8670076.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         6226 a         3392 c         4028 g         5554 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     6181 ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg gagttaaacg
     6241 cttctgtccc agcctctttt tatctaataa aattttaaga ccatttacta attatcgatt
     6301 ttttctgccg catttttagg cacttcagaa gcctttattt cttcccagct taatacacct
     6361 ttacgaggat tgactttgag tttaagatac gcattcatct ttaatggatg ttctctttct
     6421 tcgcgaagcg tcacctgttt tttttcacca tctttattat aagccgcttg cttgtaggta
     6481 taagtcgtga atttctctcc gttatctgct tgggtagctg acatttcgcc attcgtcgtg
     6541 atttttgtat aataagcttc gccgccgtaa aaataatgat aagctgcaaa acttccgccg
     6601 agaaatatgc ctaaaacgac aagtacgcca attaattttt tcatgtactt cacctctttc
     6661 cttattatta acgaaataga agatttcgtc tattgaaagt tgttggcaac agctgaattt
     6721 taaacttttc ctcattttgt tcttattgct tcaaaagaaa gttaaggact atcttgaagt
     6781 ttttctcaaa aaacacattt ttttatttat aaaaggtact tttttcgcgt ataagacaga
     6841 cgagtgctct tagttgactt tgaccaatat tgacgtataa taagcttagt tcgaatctta
     6901 caaaaagtaa gtcggacgta ttctttttga tttagtcaaa aaaagactat aataaaaagg
     6961 tgagagctga aatttttcag tgatctatat tatttaggag gtttgtaaat tatggcaaaa
     7021 gaacattatg acagaagtaa accacacgtt aacattggta cgattggaca cgttgaccat
     7081 gggaaaacta ctttaactgc tgcgatcact acagtattag gcaaaaaagg cctagcaaat
     7141 cctcaagact atgctagtat tgatgcagct ccagaagaac gtgaacgtgg gattacgatc
     7201 aataccgcac acgtagaata tgaaacagaa aaacgccact acgcgcatat tgatgcacca
     7261 ggacacgcgg actatgtgaa aaacatgatt acaggggcag cacaaatgga tggtgcaatc
     7321 ttagttgttt ctgcaactga cggtccgatg cctcaaacac gtgaacacat tttattgtca
     7381 cgccaagttg gtgtaaaata cctgattgtt ttcttgaaca aagttgattt agtcgatgat
     7441 gaagaattga tcgatttggt agaaatggaa gttcgcgagt tattgagcga atatggtttc
     7501 ccaggcgatg acactcctgt gatcaaaggt tccgcattaa aagcattgca aggcgatcca
     7561 gatgctgaag ctgctattat ggaattgatg gatacagtag atgaatatat cccaacacca
     7621 gaacgtgata cagataaacc attactattg ccagtggaag acgtcttctc aattacaggt
     7681 cgaggaactg ttgcctcagg tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta
     7741 gagatcgtag ggatcaaacc tgaaacacaa aaagcagttg taacaggtgt agaaatgttc
     7801 cgtaaaacgt tagattacgg ggaagctggg gataacgtag gcgtgttgtt acgggggatc
     7861 caacgtgacg atatcgaacg tggacaagta cttgctaaac caggttccat tactccacat
     7921 acaaaattca aagcagaagt gtacgtgttg acaaaagaag aaggtggacg tcatactcca
     7981 ttcttcaaca actatcgtcc acagttctac ttccgcacaa ctgatgttac aggaacaatc
     8041 acattgccag aagatacaga aatggtcatg cctggtgaca acgtgacgat tgatgttgac
     8101 ttgatccatc caatcgctgt tgaaaacgga acgacattct caattcgtga aggcggacgg
     8161 acagtaggtt caggtatcgt tacagagatc gaagcataag aaacgcgact cgaatatttt
     8221 ggtaatgaaa aatgtttaaa ataaaagagc ggtccaaaca ggatttctgt ttggaccgct
     8281 ctttgttctt caattgtata agaagagatt gtagaagcat gaactttcgt tttacttcag
     8341 tctcttttat tcatcatgag agtgttcatc tatcggtatg cagtttttaa tactttcttc
     8401 gtatccattt tttcaaacca cttattctac cttgtgtctg ctcaactggc gcattatcaa
     8461 taatcagcaa agcaatcata acatatgcaa tccaactatc tctagaatat agagtataac
     8521 gtgagaccca gtttgtagcg tacttgcttt tatattctct taatccatgg aaagaataaa
     8581 aatgagaacc aaactcgtaa accaagtatg caatcctttc ctgtaaaaaa ctctttctgg
     8641 aagttccgac atttgaaaga ggggccatcc ctaaattgaa ccattggata tgttcttctt
     8701 tcatataggc aaacaaatgc aagaacaaga aatccatact gccagaaggt gcttttttag
     8761 aatcatatcg catcagatca atcgttccta ctttattgtt cgtgtaagta ggcatgatcg
     8821 ttgcaaaagc gacaatttct tcttgggtat ttttgacaac tgcaataggc gctctttgta
     8881 gataatcttc agagaaaaag cctaaagaaa accctttttc ctttcgtgaa cccaaccaat
     8941 tatcagagat atttttgaaa gtactcattt gctcagtaga aaatggtggt tgtaagacat
     9001 caaatgtaaa tccttctcgt tcgatccgat tcaagacagc acgagtgccc ttcatttttt
     9061 tccctgaagt tgtaaaacta ttcaggtcta cataagcttc ttcccccatc ttaatgaaat
     9121 cgtagccgaa ttcatgcaaa atcataacaa tctcttcgct tgtttcgtag aaaactggta
     9181 aatagcatag tcgatctgtt tcttcgataa aagcttcgat tgcttctgga aaatcttctt
     9241 tcttaccaga aggatcaccc attacaatac atttattatt gaagcaagcg aattggagaa
     9301 gaacagttgg ttcaccgtct ttttcatagg caaacatccg cttatctttt aagaagatca
     9361 attgactatc ggagtttcct ccataagttg taaggatctt cagggccttt tcttcattaa
     9421 aggcttcgcc aatctgtttc ttttctcctt gaaggaaatg aacaaataga acgatcatga
     9481 aactaacagc gatgatggcc aacaatcccg aaaaccaaat cttttcagaa ggaaataaga
     9541 aaaaggaaat aaaatggtga cgccgatgag ggaaatccgg cagattgtat accccaataa
     9601 cgatgtaaag caaggacagt gcaccaataa tgattccatc gatcgtacgc cactcccatg
     9661 aatagatcaa ttgttcccga aataattcgt ttttagaagc aagaatgatc agaagcagaa
     9721 tagacaagaa aaccaccggt gtgaaactaa aatcacttaa taaaacataa agtaaagcta
     9781 gaacaatcaa gaagattgta ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc
     9841 ccataatgat taaaagaaat cccaatagta tactaggaaa ttggataata aaatgaaatt
     9901 tcaatgggtt caaactatga agccagcgaa actctgtaaa agcttgcggg atagtggcag
     9961 aaagtactag catgattcca gaaaagtata ataagacaac cacgatttta tgcgcaattt
    10021 ctgttgctaa ttgtttaggg ataccagaat aacgctgatc aaaagtggaa ccgatatttt
    10081 tgaagaaaaa gacgattcca atcaaaaacg gaatgatgta gtagaacagg cgatagagca
    10141 agatccacac aacgactatt tctcttggca caccaatagc ggacaacccg ataatcatca
    10201 ttacatcgaa actcccaatt tctcctggaa tcatagacac gattcctatc actgaagcag
    10261 caatgaataa tggaattgtt tgccacagat caatccggat ttccattaaa taaccaacag
    10321 aaaggaaact aaacaggaca ccggtccatt ccaacaatga aactagcaat agctgtcctt
    10381 gagttttaag tgaggtacca ccaaggaatc ctttcttttt gaaagtagta aataaaaaga
    10441 cgacaggaaa atatagaccg ccgccgatca accatatcca atattgcttc aaaaaaggag
    10501 caactggcgt gaagactaca agtaaaaaag aaagcagact gtaaatagaa agtccagcca
    10561 tcaaaaacaa gaaaactttc gataatgctt ggatcacgtt tctgctctct ttcttttgtc
    10621 catatagttc agagcgaagc cccacactaa caaacccgcc gaaaccggca atattgttga
    10681 tcgtattgat cagccagctt gtctcaaaca ggtaacgagg attctgcttt tgttcaagca
    10741 tccgattcaa aataatatca taaccaacca taggtaacac agagataaga ccaatcaata
    10801 gcattagtcc gcttttccat acaggtatag tggcaaacgt ttctcctaat tgctggatag
    10861 atagagtttt accgatggac atcaattcac cgacaataat gacaatgaca gaaatcaaaa
    10921 agattgtttt gaacagtcct aggtggtttt tcagccattg tatggtattt tttaacaaat
    10981 aaatcacctt ctattcctaa tagttatgtt tcagaaagaa attgaagaac ttctcgattg
    11041 aagcgttcag gatcttttct agctaattca tgtccttgtt ctttcacaag cacaaaggaa
    11101 gcttttggta tagttttagc tatatacaaa gaatgtttta gcttgattac atcttttttt
    11161 cccacgatga tcaaagtggg ggagttgatt ttttttaaat cattttctgt aagcccgatg
    11221 tcatgcagca aaagtttgat aactaaaaga tttttgcgaa gacttgggcg aaataaagag
    11281 agtaaccata cccatgcata atgaatatta ctgatgactc tggcagaaaa acgaacgcct
    11341 ttaacaagcg tgtttccaga atttaaaatc aatcgatgaa cttttttagg gaagctgctg
    11401 gcaaatacca gtgctagatt tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt
    11461 ttttccagca acatgatcgt attcagatcc tcggccatta actgaaaatt caacatagat
    11521 gcttcgtttg tcgaacgacc atgtcctcgg ctatctacta gatacacggt atagtagcgt
    11581 tcgagtacag ggacctgttc tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa
    11641 aagagaggga agccttgacc gctcttttca tagtaaattt cgctcccatc tggcattttt
    11701 gcatatttct tttctttttt cacataatca cctactgtgt aaagtataac gattatatca
    11761 gtcattgggc aattgttttt actgaaaaac aacgatcatt ttcgtaagat gtcaaaaaat
    11821 acaggaaaat tcattttttc ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa
    11881 gataaagaaa tagtaagata aaaaaagatg tcaaaataac taggtgatga aatgaagcga
    11941 aaacgattat ctattatccg tattcttttt tacatgatca ttctgacggt tattggtgcg
    12001 gtgttgtttt tatgaggaag aacttatcgt tcggtaaaac gagtggaaag ctatcaaata
    12061 gaaataacaa cagctgcaaa aaattatcat atggaaaact acgagaattt gatcaaggcg
    12121 ataatattga ccgaatcaaa aggaaaaggg acagatctca tgcagagctc ggaaagtctg
    12181 catggagaag ctgagatcat tgataatcca gctgacagta ttgatcaagg tgtcaaatat
    12241 ttggcggaaa tgatagtaga agcaaagaaa caaggatgcg atttatggac agctgtccaa
    12301 gcatataatt ttgggaaaga ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca
    12361 gtagaattag cagaagcgta ttctaaagat gtgctgagcc cacttttagg aaatgaggaa
    12421 gggaaacaat accgatattg gcggatccag tccttgcttt acaatggcgg ctatctctat
    12481 cataatggag gtaacatgtt ttatcctgat attgtcaaaa tgaatgaaaa aataatagag
    12541 acctatgaaa aagtctttaa gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa
    12601 tgaaagaagg agtgcgcaaa tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg
    12661 gtacacaacc aaagactgga tgttacggtt tgtgaaggga atgtttatcg gttcaggatt
    12721 tatcttgcca ggagtcagcg gtggtgcatt agcagctgtc tttggcatgt atgagcggct
    12781 gatttctttt ttagcccacc ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc
    12841 tgttggtcta ggcggagtga caggcatctt tctactttct tttttagtca gttttttatt
    12901 aggtgcgtat gaaacaacga tattatggtt ttttgtcgga tgtatcgctg gaactgttcc
    12961 tgcgttatgg aaagaagcag ggaaaaaagg aagagatact tctgatgtga tcatcatggt
    13021 tgttactttt gttctggctt atttgttttt attatacggc gctcaattat ttgacgggca
    13081 agtcgatcag aattttggaa catggctgat tgccggcgga ttgattggtt tgggtatgat
    13141 cgtcccagga ttgagccctt ctaatttttt ggtgtatatg ggattgtata aagcgatggc
    13201 tgacggattt aaagaagtac gacttgaagt gattgtgccg atcggaattg gtgggattct
    13261 ttgtgttctt ggattgtcaa aaatcatgga ttacattttc tcaaaggcat atagcaagct
    13321 attccatttc attttaggtg tagtttttgc ttcaacgatt atgatcattc ctaccgatta
    13381 cgcgggattg ggagttgctg gggtactagc ttgtcctgtt ctttttatag ccggtacagc
    13441 actaggttgg tggatgagcc gtttggagga acaatataag taaagataga ttgataataa
    13501 aaacagcaca cgggtgattc aaacaaaaag gttggcatct tttttgtaaa atcattcgtg
    13561 tgtttttcta taaattttat aaaaacaagt tgtttttaat cttggaacac gctagactta
    13621 aacacgtgat tatttttgaa agttggcgga aaatggagaa aaagaaaatg aatggactta
    13681 cacagcaaga ggtagttcag aaaatggctt ctggccagca aaatgactac caggaagatg
    13741 catcaaaaag tacaaaacaa atattcagcg ataatatatt gacgttattc aattttctga
    13801 actttggtat tgggatttgt ctgttatttg taggtgcgta ttccaacatg gcgtatttag
    13861 caattattgt cgtcaatatc gttattggaa tttatcaaga aatacatgca agaaatcttg
    13921 tacggaaatt atcgattgtt tcccaatcag aagtactagt gatccgggac ggtaaagaac
    13981 aatcgatttc tactaaagag ctggtattag gagatttggt aaaaatcgga gcaggagaac
    14041 agataccagc cgatttacag gttatatctg gacgggcaga agcaaatgaa gctcttttga
    14101 caggcgaatc tgatttgatt gtcaaagaaa ctggagctga attgctttct ggaagctttt
    14161 tgacaagcgg ttctgtcatg gctgaagtta tccgagtagg tgcagataat tatgctgtcc
    14221 gcttgactag cgaggcaaag actcacaaag ctattcgttc tgaacttgta gattcgattc
    14281 gcaaagtatc caaattcact agtttcgtga ttattccttt ggggatcata ctatttttgg
    14341 aagcactgtt catccgcgat gcttctatca aaatttctgt tattgcatct gcagcagctt
    14401 tgttaggcat gcttccaaag ggtttggtat tgctaataag tattgcatta acgacagggg
    14461 taaccaaact ggcaaaaaaa cgaatccttg ttcaagacat gtattcaatt gaaacgctgg
    14521 cccatgtcga tacattatgc ttggataaaa cagggaccat tactgaaggg aaaatgagta
    14581 tccagcgggt agaagcacta catccgctgt acgagaaagc catcccagaa attatgggga
    14641 cgtatctggc agaaagcagt gataacaaca tcactatgcg ggctatgaga gattattttg
    14701 agctttctaa tcggtatgat gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg
    14761 gcgcgatgga atttccagaa ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg
    14821 aagaagatcg gcttccggac agtctaaaaa cagcccaagc gaatggctat cgcgtactta
    14881 tgttggggat cgctccaaat cagcaattga acgaagaaaa accagcattt gttgagccgg
    14941 ttgctctgtt tgaaatcgat gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc
    15001 ttcatgagga aggtgtggat ctgaaagtga tttctggaga caatcctttt actgtctcca
    15061 atatcgctag acgtgcagga ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta
    15121 ctgaagaaat ggaagtgcga gaagctgctc atcgctatac ggtttttgga cgagtgagtc
    15181 cgcagcaaaa gaaattgctg gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga
    15241 caggcgacgg tgtgaatgat gttttggcgt taagagaagc agattgcagc attgctatgg
    15301 cagaaggtga tggagcaaca cgtcagattg cgaatctggt attgttggac tctgatttta
    15361 ctacattgcc agaagtcctg tttgaaggac gccgagtggt caacaatgtg acgaaagtct
    15421 caggcatctt ttttatcaaa acgatttatt catttatttt atcgattata tgtgccgtta
    15481 cagcaattgg atttccgttt atccctatcc aaatcacgct gattgattta gcgattgaag
    15541 ggtatccttc cttcttttta tcttttgaac aagataaaag aaaaatcact taccgttatt
    15601 tgcctacagc acttattaat gctttaccaa atgccttgct tgtagtagtg aatatcattg
    15661 cggtgtatct acttggaaaa acattcgcat ggggaaatgc tgaaactact actttgatgt
    15721 attacttgct gattgggatc agttgcatgg cagttgtaaa agcttgttat cccttcaacc
    15781 cattgcgcat cttcctagca gtaacgacga tcgtagggat atatgtagct gcaatgctgt
    15841 tccatggaat tttggaagta aacttagggt tgggagagag cgtaggatac ttctttgtat
    15901 tcatgctcgt aaatattgtt cttcgagtat tgtactggaa ggctgatatt caaaatcagt
    15961 tactgaaaag agtagcaaat tgatgcttaa tgagatagaa atgtgttata cttgaaaaag
    16021 caatgaaata tgatacaatc aaacatgtat aattgtgtcc tttaaaatcg cactgatttc
    16081 agttagtgcg attttttacc ggttattctc ctgaaatatc ttctgttaaa cccaaaagat
    16141 atcaatagag cagggggtga aaaaatggct caacggattc ctcaagaggt tatcgaaaca
    16201 gtacgcagtc aaacgaatat cgttgaggtg attggacaat atgtccagtt gaaaaagtct
    16261 ggtaaaaatt acttggggct gtgtcctttt catgaagaac gtacaccgtc tttttctgtt
    16321 gcagaagaca agcagatttt tcattgcttt ggttgtggta aaggcggaaa cgtgtttact
    16381 tttcttcaag aactagaagg tctaagtttt ccagaagcag tggtaaaagt ggcagacttc
    16441 gaacagatac cgttagaaga tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa
    16501 tctgctacgg ggaaactgat tgctgctcat gaaaaagcag cggaaatcta tcatcacatg
    16561 ctattgcata caaaagctgg acagccagct ttagaatatt tgcaaagtcg tgggttgtct
    16621 ttagaattaa ttgaagaatt caacataggt tttgcaccta atgagcgttc ttttcttcag
    16681 caagtattca agaatgaatc aatttcaaat gaattgttag aacgctctgg attgtttgtc
    16741 acgcatgatg atggccgtat gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt
    16801 aattctcaag gccagacgat cggtttttca ggacgctggc ttgcacctga aaaagggcag
    16861 gaaaaagatc agcccaaata cttgaatagt ccagaaacag aactgttcaa taaaagacaa
    16921 gttttattta atcttgataa agcaagaagc gatatccgga aaaacggaga aattttgttg
    16981 ttcgaagggt ttatggatgt gattgctgca tggcaggctg gtatcaaaac tggattagct
    17041 tctatgggga caagtctaac agctcaacag atccaacaaa tggaaaagct gacgaaagaa
    17101 ttggtctttt gctatgacgg agacaacgca ggttttgaag caaccaatcg agggattgaa
    17161 ttgcttcggc aaaacagtcg attagcactt tctgtcgtag tcgttccaga aaagcttgat
    17221 cctgatgaat atctccgtaa gtatggagaa gcatctttcc aagaattagt ggctcatggg
    17281 cgcgaaacag tctttacttt taaatcacgt tatttaaaac aagggaaaaa tttagataat
    17341 gaaaaagaca agatcgaata cctcgaagaa ttactagttg aacttagccg agttgtttca
    17401 ccaatcgaac aagatatgta tctatctcaa ctgtcaacag agtttcagat cggcagagag
    17461 acgatacaaa aacaactgcg cgatcttcga aggacaaatc agcaaaatcg tcaagaacct
    17521 caggaaagtc aacacactca ggttacgaga caagaaactg cacggaaaaa gagacctttg
    17581 acgcaagtag aaaaggcaga gcagattcta ttatatcgtg catttaaaga agtttctgtc
    17641 cgtaatattt taaaagaacg agaatttcag tttattcatg atgcatatgc agaagtttat
    17701 tttttattcg atgcatatgt ctcacaacat tatgaattca accttgcaga gtttttagac
    17761 tttttgcaag atgaaaattt aaggcggttg gtggtggaaa tcgaatattt accggtggca
    17821 gaagaaagtt cgccacgaga aatccaagac ttgctagatg tgatcagcaa atctggctta
    17881 gctgatgaaa taacattaaa aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa
    17941 cagcttgagc ttgaattgac gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc
    18001 aaatagctac tcaagggggg ccttctttta tggcaaatgg aacagataaa aaaacgtatg
    18061 aagcagcagt agcagctttc atcaaagaaa acaaacccaa acgacaagtt gtatatgatg
    18121 atttatccaa tcagcttgct acaccattca ctttgaatgc agatgaaatg gacaaattga
    18181 tccaaaagat tgaagacgca ggtatcagtg tggtggatga aaatggtgat ccatcaattc
    18241 acagcttgaa gagtgctgag aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc
    18301 caaccggtgt aaaaatcaat gacccggttc gtatgtatct gaaagaaatt ggccgtgtct
    18361 cccttttaac tgcggaagaa gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag
    18421 aagcgaaaca gcgtctggca gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt
    18481 atgtaggacg aggcatgcag ttccttgact tgatccaaga aggaaacatg ggactgatga
    18541 aggcagttga aaaatttgac taccgcaaag gatttaagtt ttcaacctat gctacatggt
    18601 ggattcgtca agccatcact cgagcaatcg ctgaccaagc acgtacgatc cgtattccag
    18661 ttcatatggt tgaaacaatt aataaattga tacgtatcca acgtcaattg ctgcaagacc
    18721 ttggaagaga acctacgcca gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag
    18781 tccgagaaat cttgaaaatc gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag
    18841 aagatgactc tcacttaggt gatttcatag aagaccagga agcaacaagt ccagcagaac
    18901 acgcagctta tgagttacta aaagaacaat tggaagatgt actagataca ttgactgatc
    18961 gggaagagaa tgtccttcgc ttacgttttg gtttagatga tggccggacg agaacattgg
    19021 aagaagtagg gaaagtattt ggtgttactc gggaacgtat tcgtcagatc gaggctaaag
    19081 cattaagaaa attgcgccat ccttctcgtt caaaacaatt aaaagatttc ttagaataag
    19141 cgtggactat tgaccagaag agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa
//
LOCUS       DAYYDS010000049        19115 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000049 DAYYDS010000000
VERSION     DAYYDS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.049"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..703)
                     /locus_tag="ACHA02_001750"
     CDS             complement(<1..703)
                     /locus_tag="ACHA02_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8670077.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="ACHA02_001751"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="ACHA02_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="ACHA02_001752"
     CDS             1758..2837
                     /locus_tag="ACHA02_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8670078.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2834..3462
                     /locus_tag="ACHA02_001753"
                     /pseudo
     CDS             2834..3462
                     /locus_tag="ACHA02_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3572..3799)
                     /locus_tag="ACHA02_001754"
     CDS             complement(3572..3799)
                     /locus_tag="ACHA02_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8670079.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="ACHA02_001755"
     CDS             complement(3902..6490)
                     /locus_tag="ACHA02_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8670080.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="ACHA02_001756"
     CDS             complement(6510..7169)
                     /locus_tag="ACHA02_001756"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8670081.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACHA02_001757"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACHA02_001757"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8670082.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="ACHA02_001758"
     CDS             complement(7942..8808)
                     /locus_tag="ACHA02_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8670083.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="ACHA02_001759"
     CDS             8917..9558
                     /locus_tag="ACHA02_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8670084.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACHA02_001760"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACHA02_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8670085.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="ACHA02_001761"
     CDS             complement(10999..11898)
                     /locus_tag="ACHA02_001761"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8670086.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="ACHA02_001762"
     CDS             complement(11900..12697)
                     /locus_tag="ACHA02_001762"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8670087.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="ACHA02_001763"
     CDS             complement(12675..13379)
                     /locus_tag="ACHA02_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8670088.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="ACHA02_001764"
     CDS             13510..14085
                     /locus_tag="ACHA02_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8670089.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="ACHA02_001765"
     CDS             14203..14880
                     /locus_tag="ACHA02_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8670090.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACHA02_001766"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACHA02_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8670091.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="ACHA02_001767"
     CDS             complement(16943..17941)
                     /locus_tag="ACHA02_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8670092.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="ACHA02_001768"
     CDS             complement(18167..18820)
                     /locus_tag="ACHA02_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8670093.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5522 a         4136 c         3322 g         6135 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatc
//
LOCUS       DAYYDS010000050        19040 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000050 DAYYDS010000000
VERSION     DAYYDS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.050"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..1621)
                     /locus_tag="ACHA02_001769"
     CDS             complement(53..1621)
                     /locus_tag="ACHA02_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8670094.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1625..1906)
                     /locus_tag="ACHA02_001770"
     CDS             complement(1625..1906)
                     /locus_tag="ACHA02_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8670095.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(1903..2826)
                     /locus_tag="ACHA02_001771"
     CDS             complement(1903..2826)
                     /locus_tag="ACHA02_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8670096.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2823..3701)
                     /locus_tag="ACHA02_001772"
     CDS             complement(2823..3701)
                     /locus_tag="ACHA02_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8670097.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3688..5025)
                     /locus_tag="ACHA02_001773"
     CDS             complement(3688..5025)
                     /locus_tag="ACHA02_001773"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8670098.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5028..6131)
                     /locus_tag="ACHA02_001774"
     CDS             complement(5028..6131)
                     /locus_tag="ACHA02_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8670099.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6205..7713)
                     /locus_tag="ACHA02_001775"
     CDS             complement(6205..7713)
                     /locus_tag="ACHA02_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8670100.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7679..8371)
                     /locus_tag="ACHA02_001776"
     CDS             complement(7679..8371)
                     /locus_tag="ACHA02_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8670101.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8393..9367)
                     /locus_tag="ACHA02_001777"
     CDS             complement(8393..9367)
                     /locus_tag="ACHA02_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8670102.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9667..10539
                     /locus_tag="ACHA02_001778"
     CDS             9667..10539
                     /locus_tag="ACHA02_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8670103.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11121..13319)
                     /locus_tag="ACHA02_001779"
     CDS             complement(11121..13319)
                     /locus_tag="ACHA02_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670104.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13551..14543
                     /locus_tag="ACHA02_001780"
     CDS             13551..14543
                     /locus_tag="ACHA02_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8670105.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14603..16024
                     /locus_tag="ACHA02_001781"
     CDS             14603..16024
                     /locus_tag="ACHA02_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8670106.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16017..17516
                     /locus_tag="ACHA02_001782"
     CDS             16017..17516
                     /locus_tag="ACHA02_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8670107.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17555..18949
                     /gene="uxaC"
                     /locus_tag="ACHA02_001783"
     CDS             17555..18949
                     /gene="uxaC"
                     /locus_tag="ACHA02_001783"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8670108.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5717 a         3880 c         3366 g         6077 t
ORIGIN      
        1 ttcactaact agtgaaaata agttagtgaa cctcatttat taaagaatta aattacagat
       61 gatcttttcc atcatacttg aaaaactcaa aatcagcata acttcctctt tttttattta
      121 gatcatgcac tgctatcccg ataaaatttc cagtaaaccc tcctgagagt atacacatgt
      181 ttttttgcgg acctactttc ttccattctt cagttgatac gtctttcaaa tgaaactgtg
      241 ctgtctcttt ctttacagtg actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc
      301 aatcttctat ttgtgctact ccgttttctg attctatcat acgtaggcat tttcccttgt
      361 cctcattcca agtaacataa caataaatat aattggattc atttaagtac agtaccagtc
      421 cagccatctg attaaaatta ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa
      481 aatccgtttg ccgaatagct aacaaatgat gttcgaaggt agattgaaga gattcaccgg
      541 aaatcagacg taaaaaacct ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa
      601 tgcgtctggc attccagttc ttctttagct ctccttcaaa tgtatccata aactgatgat
      661 ctttttcttg ttgtacctct tcatttgtct caatcattat ttcttccatc ggagttgtct
      721 ggctatcttt aagtcttaac caatcgtcgg acgtccaata cacttcttga atagcggttt
      781 ctcttcccaa aatagccgca ccgtttaatg gtctggtaca aagatatgtc atgtaccatc
      841 ttcctgacgg ggtttgtact aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt
      901 tgtcactagc tgtcagcata ggaaaaccag gatcaagctc gtaaggacca gtaatattct
      961 ttgctcggca aactgtaact gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat
     1021 aataataccc gttacgaaaa tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg
     1081 caaatatttt atagatggga ccatcaagct tcttcttctc tgcatcatat tcttgtaata
     1141 cgataccgct ggatttattc ggtgtgtcca gacgataatc ccatagttca ttgacaagcc
     1201 atttttttcc cgtttcaaga tcatggaaca aagaaggatc aaagccgtta ctatttaaat
     1261 aaattggttc ggaccaagga cctgtgatct cagatgcagt gatcaaataa ttgtggacat
     1321 ctttaaacgg acggcctgca cttttgacat ctgtgtaaac aagataaaac aatccatcag
     1381 cataactgat ctgtggcgcc catatacttc catttgtagg atttccttgc aaagatactt
     1441 gagtggtcaa aatatccgtt gtatgtgacc agttgactaa atttttcgac tcatatactc
     1501 ggattcctgg aagccattca aaagaggaga caataatgta ataccgctct ttcactctta
     1561 atatattagg atctggattg aatcctctta aaataggatt tttcactttg atagtctgca
     1621 tgattcaggc tccttttctc attcttactc gatataaact gaatgcccca gctgctaaaa
     1681 aagcaagtga aataaacaac acaacggcaa acagaccagt catgtcctgt aataatccaa
     1741 atgtattttc taaagacaaa gttgaatgat ctacagcaag cgactgataa ctttttttaa
     1801 actctgacgc agacatatta cttaaagtaa ctgctaaccc gccaaagaaa atcaaagata
     1861 gactgccacc gaccattcct agcaatagtt cccattttct tttcatttct gaaccccctg
     1921 cttttctaaa tgtatccatt caggtctttt gatcgatgtt tcataattgt tattacgttc
     1981 agagaatttg acagtctgtt catttttctt gtcatcccaa ttatcccaac cagttgggtg
     2041 aacatgccca cctatcttac acgcttcaaa taagacattt gcatacggac gccacggtct
     2101 tcctaaatag acgtttctag tatcaggttc tgcagtcaag gtacagcttt caaaacgata
     2161 ccctgttttt tgatttttcg gcgtagatgc tgctgtgatg tatccaggat ttttactggc
     2221 taagctatgg atatgacact gatgaaaaac ggcatctgcc ccaccaaata tgaaatccac
     2281 cgttccttca atccagcaat attcataggt ctgacgacaa tactcaggat tggcagaagc
     2341 cggtgtacaa aaaggagtac catctttttg caaatcaggc aacggacccg tgcataaggt
     2401 atcctgataa ccagatagtc gacagttttt taaagatacg cgatctcctg aattgaataa
     2461 agcaacagcc tgtcctacct tttctcctgg acctgctgta ttttcgatgt gaagattttc
     2521 taatacgaca ttcgatcctt ctaagaaaaa agtagcactt cggaaagtcc ctaactggct
     2581 accgtcaaga tactcctgtt ttgcaaaacg attaccggag ataactactg ttcctgctcc
     2641 cacaagttta aaattggata atctgctttc tatatattca tcatatcttc cttcttttac
     2701 tatcaagacc tttggatggc tatccggcag gcctgccaaa taatcgattc cttcttggat
     2761 agtttcaaaa tccgtcgggg tatctatagc attctttcca attatcagtt tgtctttcat
     2821 cctcataaat gctcccttat gaattgtttc caaatatttc tcatttcttg tgtttccata
     2881 tgaccgcctg ttaaagaaaa agaagaaaag ttttctggac tacccattga ttgatacttt
     2941 ttgtccagcc cttttctcag cttcagtacg ccttcgtcca agcacatcgt atcgtttttt
     3001 ccaactaagc taagacgagg gcgcggagcg attttttctt ggattgctaa cgttgtataa
     3061 gctgtcaata gatttggcac atagtaataa aacccatgat gatctaggcg gcgatgtagc
     3121 aaaagtgttt cgatgtccac ctgtgccgct agatcaatac aaactttgac acgagaatcc
     3181 aatgctgtca cccaccagct taataagcct cccatagaaa gaccaatcgt tgcgagccgt
     3241 tgagtatctg tgtcttccct tgtctccaaa taatcgatca agctgatcac atcataaata
     3301 cgcattcccc ataacgtttt tcctgttaat aaaaattgtt tgaacaactc gctttcagaa
     3361 atacctccgc gttcttcaaa tccccaagca tcaatcgacc aaacagcata tcccattcct
     3421 gtcagtactt ctgcaaaaga tggagaagct aagtaaggag caccatggat caattcactt
     3481 ttcccgactg aaaaatctcc gccgtgagaa tgcaggtaaa ggaccgtcgg aaacggtcct
     3541 tctttttctg gataagcata gagggctggt actgtttcca attcatttaa gtacaattga
     3601 tagtattcaa gcataaaacc gttttgtttt tctcggtata atcgttttcc actaggggta
     3661 tgtctgctag atggagcacc taaaaaatca ttcaactgca taagcaaagt ttttacgaac
     3721 aaccagttca tccatatttt ctgattgatt tgattggaat ttctcaatac tgatttcttt
     3781 agtattttct aatagaaatg ctggtccctc gtgattttca atcgacatct ggttaaagga
     3841 aaccttaccc gcaaagccaa tataaaatcc ttgtctgctc atttcctcta ttcctgtcat
     3901 catagcagga acaccgggaa ctgcatcttg ggccatcgag acatcgatat tgtcaaatgt
     3961 gatttcttgt atgaattgtt ccgctaatcc ataaataaaa cctgcagaag catggacatt
     4021 ccttgccgtg atattcgaaa aatggatcct tctgaatgct gatgtccgtt catctatcgg
     4081 ataagccttt ttttcccata catacggttc tttccctcgc ggaccacaga aataataaag
     4141 attcagtata aatggacaca tcacattatc catgactata ttggatactc gaatatcttc
     4201 aacgatcccg cctcgacctc gtctagattt caatcgtatt cctcgatcag tttcttggaa
     4261 gatacaattt gaaatggtaa tattacgaat actaccactc atttcacttc ctaatacgac
     4321 accgccatgt ccatggacca tcgtgcaatt tgtgatcgtg atattttcgc aagcgatcct
     4381 ttcgtacgta tcttcagtcc ctgccttgat tgcaatacag tcatctccca cgtcaatatg
     4441 gcaattacta atacgcacat ttttgcatga ttctggatca attccgtctg tatttggtga
     4501 atccgctggg ttcaagatcg tcaagttatc aaacgttgca ttactgcaaa gaatcgggtt
     4561 gatcgtccaa cttggtgact ggatcaattt aatatctttc acagtaattc gatgacagtt
     4621 atggaaactc atcaatttag gacgaggata agcgagatta tctggttcat ttctaaaagt
     4681 atgccaccat ttttttccat ttccatctaa tgtaccgaaa ccagtgacag agatgttctc
     4741 tacattttga gcatagatac aggaagcata aacttttcga tggactcctt cccaacgtga
     4801 atgaacaaca ggataatctt ttggatcgtc agagaatttc agaaccgctc ctgcgcttaa
     4861 atgaagttcg acattgcttt ttaaaaataa agctcctgtt agaaattcac ctgctggaac
     4921 aactacggtc ccgcctccgt cacttgctgc agcatcaatg gcttgttgta ttgcttcagt
     4981 attcaattca tcaattgatg cgccaaatgt caaaatatca tacatattta taatgcctcc
     5041 acttcaatca atgcttgcaa gaatgcacca acgccttttt gatcattaca aataattggt
     5101 tcactgatgt agtaagcata tgaaccatct cgctgatctg ctccgcctaa ccctgctact
     5161 tgacagtttt tattcaagtt cagccagcct tcttttgttt ccagaacaaa ttcatcaagt
     5221 aatccttgat gtgatttctt cacttgtgga agccattctt ctttgttcag cagtcccaaa
     5281 cgtattcctt tagccattgc gtaaacaaac atactactgc cggaagcttc cagataattt
     5341 cctttccgtt ttgtttctgt tgttacttga taccatgtcc cgctgttttc ttcttgatat
     5401 ggaagtaatg cagccagtgt cttttctaga atttctttca gcaaagaaca atctgtttct
     5461 tcatgaagta attcataggt atctgctgca gccatcaaat accagccaat cgatcggctc
     5521 caaaaatgat gggatagacc tgtttttgga tcagcccatg gttggacctt cttttcatcc
     5581 catgcatgaa tcagcaaatt tgtttttgga tcgaatagat gtttataact gatttcaaac
     5641 tgtctcaaaa catcttggta atctttttct tcactaaaat tctggataaa ttcagcataa
     5701 aaaggagaac ccatatataa gccatccaac caaatttgat aaggataaat ttctttatgc
     5761 caaaaagctc cttcggatgt gcgtggatgg tttgccaact gctgggaaag gagttctgct
     5821 gcttttttat atttttcttc gcctgtttct ttgtacaata aaaagaaaag ctttcctgta
     5881 tttacatggt cgatattgta ttcttcaacg ctgtagccac ggatcgttcc atcttcttgc
     5941 acgaaatagt cgatattgtc ttttatgaaa tcaaaataaa ttttttcttg ggttcgttcc
     6001 caaagtaatt ggaacccttt aagtacgacg ccatagtcat aagaccattt tcctttatac
     6061 cacttttctt catataagcg aggtgttctt tccatgatac tttttgctaa tttctttgac
     6121 cagttttcca ttatttgctc tcctttaaaa attaaaatag gatttgggac aaataccaag
     6181 aagtatttgt cccaatcgtc tatattactg tgccgctgct ttgtatgctt cctcgtactc
     6241 ttgtgtgatt cgatcgcctc cttgtgcttt ccagtcttct acagcttttt tgaatccatc
     6301 ttcgtcaagc tgtcccatga tgtattgcac cgtagcatca atgacgattt tttcaatttc
     6361 tgatcctact tcattgttcg tcggtgaatc caatggcaca gaaggatcca ttacagcaaa
     6421 ttcttcattt tctttgatca attgattaga gaattctttt tcagggtctg tatctttgaa
     6481 agagaacgtc acttcactag gacgagagct tgataacggt tgcacctctt gttgccaaag
     6541 atccatatcg ataaattcga tagaaccatc atcattcaat tcataatgcg ttccttcgat
     6601 accgccagtc atcaatttat acacatcttc ttctaaaaga tcatcaacaa attgaagtaa
     6661 tttcttcaat tgtgcttcgt cttttacttg agatttggga aatgctagca agccgccaat
     6721 accatttcct tctgaccaaa catgataacc gccgccttca ccatctgaga tcttgtttac
     6781 aggcactaat tccatatcat cttgaatacc ttgacccatc gtcttcagat tcttgatatc
     6841 caccattcca gtgtagattc cagttcttcc ttgtgcaaat tgttcttgct gatcagtctt
     6901 ttgggtaact gcaaagtctt gtgccaaata gccattctca aatagatctc ttgaaaaatt
     6961 caaagctttc atatactctt ctgtatcaaa ttctggagtg aatttgccat tatcgtctac
     7021 agcccagcca tttggtgctc caaaataaga agtgaaaagt ttaaagctgg tataacgaat
     7081 atcactaggc gcacgatcgc caaaaccaac tgtgtcgtcc acaccatttc catcaggatc
     7141 gttttctgta aattgtttag ctacttcata aagctcatcc atggtagttg gaacatctag
     7201 tcctagatta tccaaccagt cttttcgaat gactaagcca gcacgagcgt agtctttttg
     7261 tatcggtacg ccgtacaagg caccgtcgat actcgcagct tcgattctat cttcagaaat
     7321 ttgacttaaa tttttatagt cttttaaata ttctgataca tcccagaata cgcctgattt
     7381 taacgcattg cggacagaag agtttttcaa catcgcaatt tgaagtgtaa cgatatctcc
     7441 tagttcatca gaagccaatg cagttgttaa tctttcttct tttgaagcat caggaatcca
     7501 gttgaatgtc agttccgtat cggttttttc ttcgatcaag ttcacaatcg tgtctgtagg
     7561 cggtgaagca tgatgcaaaa tatttaacca agtgatttta gcttttccat cttctgtctc
     7621 tgaagaactg ttacttgaac tgctgccgca tgcggcaagt gctagaattc ctgcaaaact
     7681 aagacctaat tttacgagcg ttgctttttt cataaatttt ctccttcact gttttaataa
     7741 tatttttgtt ttcttctgtt ttttctgtat atgagttctt ccattccaaa tggcaaaata
     7801 tagaatttgc tgtccacgtg actgcggaaa agaagagact gccaccgata aaggttaaaa
     7861 cacctggcat tgtttggatg atcatcagat aaatccctac catacttaat atcaacacta
     7921 atgagtaatg aagatgtgag aatccgaata aaaatgccat tttcattttg aaaaaatatc
     7981 ctttccaatc ataatgagcc atgattggga aaatatagag tgctgagagc acatatatga
     8041 acgcagcaat atacaaagcg atcctaagat accaattgtg tatgaaagaa agatccacaa
     8101 gtaaaacaat cccgatggcg ccatatatcc agctcatcag caaggtttca cgatagtttt
     8161 ctttaaaata tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt
     8221 ttctaatcac tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg
     8281 cgatgactaa accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc
     8341 ttccgtttat tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa
     8401 cgccgtcttc tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta
     8461 cgaccccttt aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag
     8521 ctgtataaat ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca
     8581 agtacatatg ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa
     8641 tgattactgg acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc
     8701 catccatttc tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaaggtaga
     8761 tgatcgtaga ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc
     8821 attcagggga tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca
     8881 atacccctcc gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca
     8941 tgaagtgagg gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt
     9001 catttaatag taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga
     9061 acaagatcaa cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga
     9121 aatgtttgaa accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa
     9181 aagcaataac gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg
     9241 gcagaatcat cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc
     9301 ttctgagctt ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac
     9361 tttccataag cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata
     9421 actatctgaa ttacgtatat aatcattttt atcgtaagta aaaacgctta catagcaatg
     9481 atttttgtgc tatacaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa
     9541 accttctatt tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc
     9601 accgatgata aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct
     9661 atctatatga aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg
     9721 tttctaattt tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct
     9781 tttgcttcta cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct
     9841 ttagacgcgt atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg
     9901 attggtgtta caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac
     9961 ggtctttcca gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct
    10021 atgttgttca gcggggggat gattccaacg ttcttagttg ttcgttctct agggctggtc
    10081 aattcttact gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg
    10141 agaaactttt tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc
    10201 acagattttg gtgtattttt caaaattatg ctgccattag ctttaccgtc tattgctact
    10261 atctcgcttt tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc
    10321 aacgattcat ctaaatggcc gatacagatt cttttacgcc aaatcgtcat tgtatcaagt
    10381 gggatgcaag cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg
    10441 gctgttatcg ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat
    10501 tttgtcaaag gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg
    10561 gtagttgtgt cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc
    10621 ttaaattttt gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta
    10681 tcagaggttg tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg
    10741 cgacgagtaa cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca
    10801 caaaattatg ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga
    10861 actgtgacaa gacttttgtc acagttcttt ttgcgtgctt agccttgaaa tttaactatc
    10921 cataaataca aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga
    10981 aaaacagcct cttgattaaa cggcgtttat cttgttgatt ctgcggcaat aaaatcagcc
    11041 agcagcaaaa catgaagtgc tgttttttgc tgtcctcttt tattgcttga atcagatcac
    11101 aagaaaaaca gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat
    11161 tccttctttt taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca
    11221 atctctttta cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga
    11281 ttttgaacaa aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag
    11341 ttagggttat aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct
    11401 gtgtagacga tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct
    11461 tgagagcatt ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg
    11521 ggatgataat tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa
    11581 tctactccca ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact
    11641 tcccgactca aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa
    11701 ttcttcactt cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc
    11761 ggatatctaa taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt
    11821 gaacttggac cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc
    11881 ttgctttcat tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag
    11941 tattctttta cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct
    12001 gcatcatctt tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc
    12061 agcattcgat tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc
    12121 aaatcacgct ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa
    12181 tagcagtcat tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt
    12241 tctactcgat ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta
    12301 actaagtgtg cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc
    12361 aaatctagtt ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga
    12421 gaaatcggac gggcaaatct gtccgaaaat aagtaggaaa aaagaccaat gagtcctacc
    12481 agtgttaaag acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca
    12541 ctagagtcaa cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac
    12601 gttttatctt tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat
    12661 acagttggtt gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt
    12721 tcaatagaaa gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct
    12781 atcgtagcaa ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt
    12841 tcgattcctt tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt
    12901 tgatactttt caacttcctc ttcggtcagt tgactcaacg atccattgat tactccccat
    12961 ttaccagcca aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa
    13021 tttagttctt tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg
    13081 aatatgtccg agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta
    13141 attttccatt caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa
    13201 ctcgattcga tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt
    13261 acggctccta ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt
    13321 ttcttcctct cctcctttta aaataaaagg ctttcatttt tctgtaaaat tgatactcat
    13381 ctaattaaat gagcattccc cgctttcaag aatagcataa ttaaaaataa aaatccaagt
    13441 aatttttaac gtgtgcaatc ttttcatatc aaagatctat tgacttatcg tagttatcgt
    13501 aatatgatga atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga atggtaacga
    13561 tcaaagatat cgctaaagca gctggcgttt ctcatacgac agtttctcgt gctttgaata
    13621 acagcagttt aataaaaaat gaaacaaaac aaaaaatacg acaactagct aaggaaatga
    13681 attattcgcc aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat ctaatcggac
    13741 tatttttctc aagtttgcat aaaggaactt catcaaattt cctagcagat tccattgcag
    13801 gcatccattc agtattagac acaaattaca gtttatctgt tgaaagtatc gatgttgttg
    13861 atttaacaga aatcaacctg caacgctacg atggactgat cgttatgagt caaagtgatg
    13921 atgatcagcc tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt gttgtcaatc
    13981 gtcacatcaa tgatccagcg gttatcaacg tcactgccga tgatgcggcc ggtgttatgc
    14041 aggcagtaga ttacggtatt ggatgcggac atacgaagat cgcttatatt ggcggaaaga
    14101 aaaattttcg ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta aaaaaagcga
    14161 gcatccctgt caatctagcc tattttttaa caggagatta cagcttagaa agcggcctcc
    14221 aaaacatgga atacctatta tcactttctg acctgcctac tctggttttt tgcggcaacg
    14281 acgacatagc gattggtgca ttgagagctg tacatttagc tggcctccgt gttccccaga
    14341 acatttctct gatcgggttc gatgattctc cagtcgtctc ctatctcaat ccgccgctta
    14401 ccaccgtacg taagcctttg aaagaaatgt gtcaagaagg aacaaagctt ttactagatt
    14461 tgatcgaagg caggacgatc gaacaatcta aaattgagct ctccaccttg ttgatccttc
    14521 gtgagtcgat caattacttg taaactacaa gtaattttat ataaaaatgt taacgtgtgc
    14581 ataacacaga aaggaagttt atatgaaaaa actgacaaaa gaattagtga aaaaaaacga
    14641 agcacctgta aaagttcttc agtttggtga aggcaatttt atgcgtggat ttattgattg
    14701 gcagattcag cagctgaata accaacaatt gtttaaaggt aatgttgcaa tcattcaacc
    14761 tttagaacaa ggtcttggaa atatgatgaa ccagcaagat aatttatata cggtcatttt
    14821 acaaggactc agcaatggcg aaatcgttga tacttccgag attatcaccg tagtagaaca
    14881 aatgatcaat ccttatgatg aatgggatgc ttatctatct ctagcagaaa atgacgactt
    14941 ggcctatatc gtctccaata caacagaggc aggaatccaa ttcgtggaga ctgataattt
    15001 aacagatact cccccacaga gcttcccggg caaactgaca gcctttttat accgacgttt
    15061 caaattaaac aaagctggct ttaccatcat tccttgcgaa ctgattgaca gaaacggcga
    15121 acgtctaaaa gaaatcattt tacgctacgc agaaaaatgg gatttagaac ctgcttttgt
    15181 tgagtggtta gaaaaagaaa atgttttctg ttgtagttta gttgatcgca tcgtcccagg
    15241 ttatcctcgc gaagatgcag gtgttttcaa tcaacggcat aactatgaag atcaattgat
    15301 ggtcaaagca gaaccattca tgctttgggt gatcgaaggg cctaaagaat tggagaatca
    15361 actaccattc aaacaggctg gcttgaatgt catcatcaca gatgatatga ctccttatcg
    15421 tgaacgtaag gtgcatttgc ttaatggacc gcatacagcg atggtccctc ttgctttgtt
    15481 ggcggaattg gaaactgtag aagaagtaat gaaagatcct ttattctccg catatgttga
    15541 tcagttattc aatctagaat tgattcctat gctttcttta cctaaagatg aattagccat
    15601 ttatgctgac caaatcaaag aacgtttctt gaatcctttt gcccatcaca aattagaagc
    15661 gatttcatta aatagtgttt caaagttcag cacccgtctg cttccagtgt tcaagaagta
    15721 tatcgaagaa caaaatcagg taccaccgtt aatcactgtt tcactagctg cgctacttct
    15781 tatgtatcga ggcgatcaag taaagccaca tgatgatgaa aagacgataa gcgaatttac
    15841 tgatgcttgg tctgataatg gaacagctat tcctcgatta ttgcaaaacg ctgcgctttg
    15901 gggagaagat ttgtctcaaa taccgaatgt gacagataca gttcaagaaa tagctgcaaa
    15961 gatcgatcaa aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga tggtccatgc
    16021 ttgaaaccct gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc gtcgcattaa
    16081 cccctattcc aaaacaaaca caactgacga ttgatcagca aacgatcacc actcttgaag
    16141 atatcccgca aggtcataag attgcattgg gtgatttgaa agctggagac aacgtgatca
    16201 aatatggcta tccaatcggt catgtgacga cagacgtaac tgccggtcaa tggctgcaca
    16261 ctcataatgt aaaaaccaat ctttctggtg aattggatta tcgatatgaa aaagatgtcc
    16321 atccaagtca ttaccttttt gagaaccgaa catttcaagg ttacttgcga aaaaatggaa
    16381 aagtagggat tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg aatggcattg
    16441 ctgaactgat cgtcaaacag ttcaaggaaa aacatccaga tctgggacag tttgaccata
    16501 ttacgatttt aaagcatcct tatggctgtt cgcagcttgg aaaagatcat caaaatactc
    16561 gagaaatttt agcggacgca gtaaatcatc ccaatgccgg aggagtttta gtatttggat
    16621 taggttgtga gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa tatgatgaag
    16681 aacgagtgaa atttctagtg gctcaagatg tttacgatga gatcgaacaa ggtgtggctc
    16741 tattagaaga gcttttaaca gcggcggaaa atgaccagcg catatcagtc ccgctctcta
    16801 agttgaatgt cggtttgaaa tgcggcggtt ctgatggtct atcgggaata actgctaatc
    16861 ctttacttgg tgcattttct gactacttga ttgctcaagg aggcagcacg atcttaacag
    16921 aagtgccaga aatgtttggc gcagaacaag tattgatggc acgagcggaa aatgaagaag
    16981 tttttgactc aatcgttgac ttgatcaatg acttcaaaca gtatttcttg tcttacgggg
    17041 aaccagttta cgaaaaccca tctcctggta ataaagcagg tgggatcact acattagaag
    17101 ataagtcttt aggctgcacg caaaaatcag gaagctctcc tgtcgttgat gtgctacaat
    17161 atggcgaaaa aatcagaaaa ccagggctta gtttattgca agctcccggg aatgatcttg
    17221 tagctgcctc tgctcttgct tcatccgatt gccagttggt cttgtttacc acaggaagag
    17281 ggactccgtt tggctcatat gtcccaacgg tcaaggtatc tacgaataca actctttttg
    17341 aacgcaaagg tcattggatg gattttaatg caggcgtctt attagaacaa ccaatggaag
    17401 taatcttaga atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa gaaacgaaaa
    17461 atgaacaaaa tgacgttcga gaaattgcca tttttaaaaa cggagtaaca ctttgatttt
    17521 cataaaacag tataaaaaga aagaaggaag atttatgttt ttatctgatg attttttatt
    17581 aaaagacgat tgggcgaaaa agctttacca ttcctacgca aaaaacatgc ccattattga
    17641 ttatcactgt cacttagatc caaaagaaat ttacgaaaac aacaatttca gcaatctgac
    17701 agaagcttgg ttaggaggcg atcattacaa atggcgcttg atgcgagctt gcggcgtgcc
    17761 tgaagaaaaa atcacaggaa atgcttctga ttttgaaaaa tttttagctt ggtgtcaaac
    17821 cgtaccaaaa attatcggaa atccgcttta cagttggtca catcttgaat tgaagcgttt
    17881 cttcggtatt gacttgttga taaatgaaga aaatgcagag aaaatttgga cacaagcaaa
    17941 tgaaattttg gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat cgaatgtaga
    18001 agtgatttgt acaacagatg atcccgcaga cgacttgcac tatcatcagt tgcttgctca
    18061 tgaagaacca gatttgaagg ttctaccttc attccgtcca gataaagcac tcaatattga
    18121 aaaagaagga tttaatgcat gggtcacact actggaagaa gcagctgacc aaacgatcac
    18181 cacctatcaa gaactgatag aagtcttagg gcagcggata gaatacttcc atcaacatgg
    18241 atgtcgtgtt tccgaccacg ctttagacat cttacgctat aaagaagcgg atgaaacagt
    18301 attagaagag atttttcaaa aagcacgagc taacaaacta ttatcttcag atgaaatcga
    18361 tgcttatctt acagaaacac tgatacattt gacccacttc tatcattctc ataattggac
    18421 gatgcagctg cacatccatg cttatcgtaa ctgtaataca caaatgttgg aaaaattagg
    18481 gcccgatacc ggctatgatg gaatgaatga tctttctttg actattcctc tgcaaaaatt
    18541 attgaaccgt gcagaagaaa cggatcagtt gccaaagacg atcctttatt cattgaatcc
    18601 gaatgactat cctgcgattt tagctttgat gggctgtttc caaaaagaac agaatggaaa
    18661 actgcagtta ggttctggat ggtggtacaa cgatactcga gctggtatgc gcgatcaatt
    18721 gacacaattt gctgatggta gtgctttggg gaattttgtc ggcatgctga cagattcaag
    18781 aagtttcctg tcctacacta gacatgaata tttccgtcgt gttctttgtg aatttttagg
    18841 cgaaatggtc atgcgaggag aagcacctga agatgaacaa cttttaggaa gtctagttca
    18901 agcgattagt tatacaaatg ctaaaaatta ctttggcttt tttgcctaat tgattaatag
    18961 taacagtaac gaatcaaaaa caggatagtg aaataacgga atccgtttat ccacgtttta
    19021 gagaagaaga cggaccaaat
//
LOCUS       DAYYDS010000051        18775 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000051 DAYYDS010000000
VERSION     DAYYDS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.051"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            359..1270
                     /locus_tag="ACHA02_001784"
     CDS             359..1270
                     /locus_tag="ACHA02_001784"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8670109.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1487..1924
                     /locus_tag="ACHA02_001785"
     CDS             1487..1924
                     /locus_tag="ACHA02_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670110.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            2019..2903
                     /locus_tag="ACHA02_001786"
     CDS             2019..2903
                     /locus_tag="ACHA02_001786"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8670111.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            3127..6294
                     /locus_tag="ACHA02_001787"
     CDS             3127..6294
                     /locus_tag="ACHA02_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8670112.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            6362..6859
                     /locus_tag="ACHA02_001788"
     CDS             6362..6859
                     /locus_tag="ACHA02_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM8670113.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            6878..7342
                     /locus_tag="ACHA02_001789"
                     /pseudo
     CDS             6878..7342
                     /locus_tag="ACHA02_001789"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            7339..8097
                     /locus_tag="ACHA02_001790"
     CDS             7339..8097
                     /locus_tag="ACHA02_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8670114.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(8183..8923)
                     /locus_tag="ACHA02_001791"
     CDS             complement(8183..8923)
                     /locus_tag="ACHA02_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8670115.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            8984..10237
                     /locus_tag="ACHA02_001792"
     CDS             8984..10237
                     /locus_tag="ACHA02_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8670116.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            10559..10786
                     /locus_tag="ACHA02_001793"
     CDS             10559..10786
                     /locus_tag="ACHA02_001793"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8670117.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            10909..11235
                     /locus_tag="ACHA02_001794"
     CDS             10909..11235
                     /locus_tag="ACHA02_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8670118.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11303..12016
                     /locus_tag="ACHA02_001795"
     CDS             11303..12016
                     /locus_tag="ACHA02_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8670119.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12326..13177
                     /gene="licT"
                     /locus_tag="ACHA02_001796"
     CDS             12326..13177
                     /gene="licT"
                     /locus_tag="ACHA02_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8670120.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            13298..15163
                     /locus_tag="ACHA02_001797"
     CDS             13298..15163
                     /locus_tag="ACHA02_001797"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8670121.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15177..16620
                     /locus_tag="ACHA02_001798"
                     /pseudo
     CDS             15177..16620
                     /locus_tag="ACHA02_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            16728..17429
                     /locus_tag="ACHA02_001799"
     CDS             16728..17429
                     /locus_tag="ACHA02_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8670122.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            17555..18067
                     /locus_tag="ACHA02_001800"
                     /pseudo
     CDS             17555..18067
                     /locus_tag="ACHA02_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(18465..18674)
                     /locus_tag="ACHA02_001801"
     CDS             complement(18465..18674)
                     /locus_tag="ACHA02_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8670123.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         6359 a         3124 c         3988 g         5304 t
ORIGIN      
        1 aatatatcat aataaaaaca acaaaaaaat gtctggaaaa tcgaacataa aagtgtctga
       61 tttgaatatc ccagcagcgg aagaagaaaa acaaaatcga taacatttgt tctaagaatt
      121 agttacgtta aatacggaac gcaagataga cttgaaaaac ggtaacaaaa gaagtagtat
      181 attaaggtgt gatgtgctac ttgacattta ttctgctgag cgaactttct ggattgtgta
      241 gccttatatc tattcaggac gtatcgagca gattagtaaa ggtgttcttg atgattttca
      301 tcttgaacac ctttgttttt agcaattcgc aaaaaacgaa aagtaggaga attgataaat
      361 gaaaaaaaga tttttagcac ttgccatcgt actaggcact ggcttattgt caggctgtac
      421 gaatgccggt gaaaaaacag ccgttagtta taaaggcggt acaatcagcg aacaagaagt
      481 aatggatagc ttgaaaaaaa tgcaaggagc agattccgct gtgcaacagt tgatcgttta
      541 tcaagtattt gaagataaat atggagatga cgtatcaacc aaagaaatcg attctcaata
      601 tgatcaaaca aagaaacaat tgggtgattc atttgattca caattgaaat ctgcaggtta
      661 cacagaacaa acattcaaag acagtatcaa acaaagtcta gcctttcaag aaggtctaaa
      721 gaaacatatc aaattgaccg atgaagattt aaaaacggct tgggaatctt tccatccaga
      781 agtggaagct caaattatcc aagtagcaag tgaagatgat gcaaaagatg tgaagaaagc
      841 tgctgataag ggagacgatt tctctaaatt agccaaagac aagtcaactg atactacgac
      901 aaaagaagat ggcggaaaag tgaaatttga ttccactact acaacggttc cggcagaagt
      961 caaagaagca gcctttaagt tgaaagatgg tcaagtttct gatgtgatca cttctaccaa
     1021 tgcttcaact tatactacgg aatattatgt ggttaaaatg gtgaaaaacc aaaacaaagg
     1081 caacgacatg gataaataca aaaaagaatt gaaagaaatt gcaacagaca caaaattaag
     1141 tgacagtacc ttccagaata aagtaattgg cgaagtattg aaagacgcta atgtaaaaat
     1201 caaagacaaa gactttgaaa atgttttgag tacattcact tcagattcta gtacagcaag
     1261 tagtaaataa gcggtataaa taaaaaatat gaactattta aatcaataaa actgttcatc
     1321 taaatctttt tattagaact aaatcaacag tagtttaatt actacttgat ttagttcttt
     1381 ttattttaaa aatttttttt acttagaatc cagcgaccct agatattttt ctgtataatc
     1441 ttaaataaag aaaacaatat caaaaaatgc acgtagaagt agataaatgg agtggttttg
     1501 caaatttctt ttatttctag aagttattca cgtatacgga tttgtttttc tcacaaataa
     1561 aaaggcagga agtacaatgg atcatttgta tataaaatat attcatcaat tttccgtttt
     1621 ggtgttaggc acactaatta tagcggggat tgtcgcattt attattcaag gccgtgacat
     1681 attaacagta catcaagagt ttttcagtta tgcatttggt attcctctga tcgctataag
     1741 gtctgataac aaaaagtgga agaaacgcta ttacattctc ttgctatttt tgtgttttta
     1801 tctgccacct aagattttta ctttattagg tatacaatgc ctatcatttt cttttatttc
     1861 ctatatcatt gttttaattt tcctcagttt catagtatgg atgttttatc ttgtcaaaaa
     1921 ataaatgaat gtttcatgaa cagtacttca tcacagccta atttttttct ttatgctatc
     1981 gttatattaa agaaatcaat cagaagggaa agaaagtgat gaaaaaagtt ttattagtgg
     2041 taaaccccag ttcaggcggt gaacaagcaa aggaatttga gcaattagcg atagcaaaat
     2101 tggaatcggt gtttgatgaa gtggtcgtct tgcatacaaa aaaagcaggg gatgcaaaaa
     2161 actttactcg cgaagcggct acggaagggt atcatagtgt gtttgtcatg ggcggagatg
     2221 gaacagtcaa cgaaggaatc agtggaatcg ctgaacaaga acatcggcca aatttcggat
     2281 ttttcccatt gggtacggtg aatgacttgg ctcgagcatt agggataccg ctagaacctg
     2341 aggaagcgat caatcatttt tcgattgaat cagtcaagcc attggatatt ggaaaaataa
     2401 atgacgacta ttttatgaat gtcgtcgcaa ttggctctat ccctgaatca atcaatgatg
     2461 tagatgcgga gaagaagacg aaatttggaa aactagctta tttcatgtca ggaatcaaac
     2521 agttagcttc cacacaatcc tactcgtttc acgtagaagt tgatggaaag aaggaagaaa
     2581 ttgagagcag taccttgctg ataggactaa ctaactcagt cggcggtttt gaaacattac
     2641 ttccaaatgc aaaagtggat gatggtaagc tccatttggt ttatttaaaa gattcttctt
     2701 tgatcgatac gcttaaaaca ttgccagacc tattaaaagg agtagatgaa tctacgaaaa
     2761 atctagccta ccttacttgt gagcaattag ttgtgtcatt agctgatgag gaagaattga
     2821 cgacgaatgt tgatggagat gaaggggata aattacctgt gacaatcaaa gtattgccct
     2881 cacatttaca ggtttattgt taaaatggag gttgtgaccg aggagttggc gggagaacac
     2941 cgcttgttcg ggataaggga ctgtgacaag acttttgtca cagccttttt tttgtacaaa
     3001 aaagaaattt acacctaata gaaaaatttt tccataaaaa tacatatttt aaatatgaaa
     3061 gtacaattgg aaatagatta aaacgataaa ataaaattgt caaaacgtca gagaggatgg
     3121 tggagaatga atcaatgtgt aaaaaagctg attgcgtcga ttttgtttgt cttccctctc
     3181 ttgatggtga catccgtttt tgccgatgta gagaaaacaa acgaatcaac gcaatcagag
     3241 aatcaaagtt ttgacaagac tactttttca gaagaatcga ttacttctga aactacagaa
     3301 tctcaaagtg aggcttctac taataaaagg caagaagaaa ataagacaag tgagacctca
     3361 gaagtagctg aacaaacgtc tgatagcagt gaagcggcac tggcaagagc tgtttcagcg
     3421 gatgggtaca gcgaagctgc cacaacagaa gaactggcgc agcttctcgc agacgaatca
     3481 gtaactaaga tccgactgat tcagcctttg actttagaca gggaattaga gatcaaacga
     3541 gatatcgtta ttgattttgg tggattcgct cacaatttcg gcacacacca tatttatatc
     3601 aatgagatac ctcagtcgat cgagttccag aattttaaag gaacagctgc acatccaggt
     3661 ggattgacac caaatatcga tggaaatgcc attatcattg cttatatgac cgatttttgg
     3721 ggaagcagat atcattttac gggagaaata aaatttaccg ggacacttga tttgtatgat
     3781 gggtccaaat taggactgat ttacgctcct cgtgcaactg tcacattaga tggtgtttcc
     3841 ggtgtgttgg atgtccaacc agtgaaggaa ggactgaatg ccgctccggg taaagcctat
     3901 ttttgtagaa gctatcaatt gaatgtgatc aatggtagtc aactttatgg accgtatcta
     3961 gggaaattct acggttttct agattcagga gatgagactg gtagtggaaa aacagcgcca
     4021 ggaatccata tcttaagcgg ttcaaaggtc tctcttgatt atgatcgggc agatggcgtt
     4081 agcgatgaag gagaggcagt cgatacgcta cctggtaatg tcatgtttga agtcagtggt
     4141 tcgggaagcg aattttcggt aaatacaaag atcaatatta ccaatgacaa caatcgcgga
     4201 atcatccaaa tgagaggttc aggatcgaaa gtcactgtat cgaatggtgg gaaaatcacc
     4261 atcaatacag caactacggg cggatttcgc ttgcaaggag attctagtca aatccatgta
     4321 tcaagtggtg gacaaatctc ggtcaaaatg gctggagatg acgatcaacc cgggaacaat
     4381 gggatgcgtt ttgtaggaaa aggcctttct ttgatcgtag aaggagccgg atcagttatc
     4441 gacatcgata agcagagtgg gcgttccagc gcagttcgtt ttgagaatgg tacgcaaaag
     4501 ctgacagtaa ccaatggagg ctcattgagg gtaagaaata caggtgatgg caaatcttac
     4561 gtgaacagac tcaatcaagg aaatcaagcg atccaatttt atgacgcttc tggttttcta
     4621 gaaagtccag gttcagctga ttttacagtc actggagaaa aatcaaatat cgatatacgc
     4681 gcagattccg gagcaactgt agatacaact ggagatattg atttaaattt tacggcaggt
     4741 gaaaaaactt atttagtcat ggtcgggaaa acaggggaag ataagaccgg catcatcagt
     4801 ggggatgtgt tgaatgtgga cttagaatct cctacctatt ttgatttcca aaacaaacgg
     4861 attggaagcg agaccaatgg cataggcggc tgggtattcc aaggaaatgg agattcttct
     4921 ttaaagatcg cccattcaga aatcgcttta tggcgaaaaa caggcgcaac attcgataac
     4981 tttgaaaaag acccggatta tttttctgat atgacagact tatcgatcaa tgggaccgac
     5041 ttaggcaatt tcgtatcaag ccctagtgca ggtttaacaa atgaatttgc tcagacaggt
     5101 gccggaatga aaaattataa tcgaatcagt gccaataatc agctcccgat gattgaatcc
     5161 ttaagagtgc caacgaatgc agataaaaaa atctatgggc atgtcgttgt tcccgaaggt
     5221 gtcgaggcaa agccaagaga tgcttggaca ggcgaagtag aagtgacttt acgtattact
     5281 tacgcagata cgacgaaatc atctgtagaa attactgctc tcacgcaagg acaaacagat
     5341 gaaaatgaag gggcaggata taacccatgg ggagaaggcg aacaaggcgg cttgttcgaa
     5401 gttcaagtac ccaatgatga gagattaaaa acggaggatc aagtgcaagt tgttgctgct
     5461 aaaaaagtaa gaggtggcca agaagtgaca gaactggact ctgccaaaac gacagtcgat
     5521 gtgattcctc ctgaacctgc taatttaagt accacattga tcaatgcagc ttctcgcacg
     5581 atcacaggta caggtgaagc aggagccttg gtttcattga aaaaaaatca agctgttttg
     5641 tcagaaacaa ctattgatga tacaggggaa tttgttcttt ctatcccaga aaatcaactt
     5701 tttgcaggag atgtattaga aattgtatta aacgaccaag caggagaagc tactgctaag
     5761 ggtgtgcaga acaagccaag taccaacgat gaaattggga atcacaatcc aagtgatacg
     5821 ccagttgttt atcatgatgc cccgcctttt gcacctgcta cgaagatcgt agtagaaggt
     5881 ggtctctcat ttcttgcccc ttccaaactc gattttggtc agatcaaagc aactggtttg
     5941 acacaagaag catttggcca aatggatata caggaaaaat tatggattta tgatcagcga
     6001 gaaacgaaaa atccgtggat cttaacgata aaattatctc agcctttcac aaatgatgca
     6061 ggattttcta tgcaggatgt cctatatttc aagaaaggag aggagtatca attagtgaat
     6121 gaagaagaac tggaaattgt cagaggacaa aattcaacga atgtgcaagc agattattca
     6181 gaatatttaa ataacggtcg ggcgtttaaa cttgtcttaa ccaaagaaaa tcagatagct
     6241 ggtaattact cagcagagat aacatggaca ttagcagatg cgccgacaga ataaagatca
     6301 aggaggatga ttgatgaatc gaatatggaa taaaagtcat cgaaacaact gctctttact
     6361 tatgatcctt ctccttggat tcatcctttg gcagacacct gtccatgcct ccgaattcaa
     6421 ttttgcggta gattttgaat taccggctaa tcagattgat aagaacaaaa catatataaa
     6481 tgtaaggatg gagcctggga aaacacagga aatcccctac acggtcttta accgaacaga
     6541 taaatcagta acagttgaga cattgatcca ctccgctacg acgaattcaa acggtgtgat
     6601 cgaatatgga gaatcaaaaa gtaaatttga tgaaacatta agttatgata tgaaaaactt
     6661 agcaaaaaca gtgggagaaa taactgttcc tcctcaaagt gcggtcactg agacactttc
     6721 actgaaagcg ccagaaaaag agctggctgg tgtggtcgca ggagggatca cgtttcaaga
     6781 aaaaaaagag aacgtttcta acaaaagtac agttggtgtc gtcaatcagt tttctcaagt
     6841 gaaagcaatc ttgatttgaa atagcgaaaa agagtcttcc ccagatttga aattaaaaaa
     6901 agcttatgcg gatcaaagcg atggaagaaa tgtgattaag gcgaatcttc aaaatccaaa
     6961 tgcttcttat atgttcggct tgactacatc ggttgaaatc tacaaaaaag aaacaaaaga
     7021 gccttacctt tcaataaaaa aagaaaaaat agatgtagca cccaatacaa attttgattt
     7081 acttgtccca ttagaaggaa aaaaattagc tccgggacat tatcaagcgg ttgtgaaggg
     7141 tgcttggaaa gaaaaacgct ggaatctcca taccgatttt gaaataactg atgatgcagc
     7201 ggaccaattg aatgaaaagg atattgattt aaaaaatcag ccagaaacat ttgactggcg
     7261 atggctgata cttgttggag tcattcttat tggcttattt gctggattat ttattttaaa
     7321 agaaagaagg aaaaagaaat gaaaaaaaca attgcattaa gtaccgttct agctggaagt
     7381 ctattattat gtacacaaag tgtttttgct gagaataaga gctcagccac tacagaagga
     7441 acgatttctg tgaccaaacc agatgctgca aatcctgaag acgttgttcc agttcctcct
     7501 gttgatccaa atgatcctga tactcctttt acacctgacg aaccaaatca gggaacatcc
     7561 ggattactga cgattgacca agctccagat cttgattttg gaacaatcaa gttaggtaca
     7621 gcaaaaacag tatatgctgc attacaaagc ggaacggaca gtactggagc acaaacacaa
     7681 gtacagaatt tcgtacaagt aacggataaa agcggaaatt atgccggttg gaaattatca
     7741 gtcaaacgaa cagaatttat tggtacaaca tctgatacga ataaactaac tggttcaaag
     7801 gtgaaattca aaaacgccgt gattcgaaat agcgcaacta atagcagtgg aatgccatct
     7861 accgtttctg gaactggcgc tgaaggaatc gaaatcccag cagatcaggc agtagatcta
     7921 gtaactgcag gagcaaatga agggattggc acatggattt attcattagg agagaatgtg
     7981 gaacaaggga gacaatcagt ggaattattt gttcctgtat ccaattatgc tgtagacaac
     8041 tacgtttcaa cattcacatg gtcgttaaca gatgctccta cagaagagga agcgtaaaat
     8101 catatcaacg atttcaggga gtggagccga ttcaaggccc actccctgaa atctgctatg
     8161 cttacgtctc agctcttatt catcaactcg aagcagtttt ttggaagtca tggaaacgtt
     8221 cctttgtctt ttcaaagatc tttcgaccaa cgagtaagga gtcaccgttg aaaaattcta
     8281 acacataatg agcagaatca attttctgca attgcgataa gttgatacag taagatttta
     8341 aaagtaaaaa gtaagcatac ttttctaact ctgtcttcaa ttgttttaaa gaagtattca
     8401 ctaatttatc ttctgtagtt gttttgacca taacttggtt tttcaaacct ttgatacttt
     8461 caatatataa aatttcccga ggatacagga cgagacttcc acctttttga tcaataaaga
     8521 ttttatcgac atttacgatc tgctgttctt tccaattgat ctgttctagc acttgacgaa
     8581 tttgttcaga taaattcctg tcttttctga tatatccaat cggaaggatt ttccgattga
     8641 tgatctctac tgcaaaattc gtataagcgg tgaaaaaaac aatattacta tgtgcatttt
     8701 ttttacgtat ttgttcggcg acatcaatac ctgtgagtga ttgcttcaag tcgatatcga
     8761 taaagaaaac atcgtgatta tcaatagtgc ccaactggat caactgattg atttgagaga
     8821 tagaagtgag ctgcacgata ttctcgatat gatgattgac caattctctg atgatccatt
     8881 cgctgtgaaa ctggttatct tctatgataa aatacttttt catatgcagg ctcctattaa
     8941 aaaaatatgt gtacattttg aaacaaaaca atggaggaat aagatggtcg caacagtgac
     9001 actttatttg acctttactt tgattttaga tttaggtatc tatttattct tttcgaaaca
     9061 actgctagta aaaaacagct ttggttgtat agctttgatg gtggtgacac ttttacttcc
     9121 tatccctaaa gtgagctttc cttacctagt tggcttatta ttgatgtttc ttttttattt
     9181 tactacgaaa aacctgttta tcagctgttt gatcagtaat cttttttttg ctatcactgg
     9241 actcagcttt tttctggttt ttgatctgat ggattattta ggaaagagac atcttggaac
     9301 agcaagtttt tttgcactac ttatcgcatt tattttctat ctattagtaa aaagtataga
     9361 caaacagacg agatttctca acttgctttt tacttatcca actgaacact attttggagg
     9421 tatttgtatt ttattaagct ggggcgtgat ttacttggta ttgggaagtg ttcacttccg
     9481 ctcatctaat tatctgcttc tatctctttt gtcgatagtc atcagttttt tttctttgtt
     9541 tttttccatt atgttgatca gtttccaagt aaaagaaaga caacatcagg aatcgcttca
     9601 actgtatcaa ataaatgaag aatattacca tcatctagag gaattcaaac atgattataa
     9661 aagtctactg ttttcattaa aaattactct tcaggaaaat aaaaaagaaa cgttggaatt
     9721 tttagaaata ttagaagatc attctgcagc tatttttgaa aaaccgcaaa tacaacagct
     9781 aacaaatatt gtatccccag ctgtcagggg tgtcttttta aaatttctag aaaaagcaga
     9841 atcggagaaa atcccgatcc aagtagttat tcctgtcgaa gtgaaaacaa tcccgattga
     9901 tctggttgtt tttattcgct gtttatctat ttttatttct aacgcattcg atcatcgtgt
     9961 agccgatcag agtccgatta cgatcaatct gctggaagaa aaagagggat cacgttttag
    10021 tatagcgaac aaggcaaatc caacgaattt gacgctatcg gagatggtca aaagaggaaa
    10081 aacctctaaa aaaaatggcg ggttaggtct atacagtgcg aaaaaaatgt tggatgttta
    10141 cgaaaatgca gaactaaaaa tcgaattttc aaaacagaat cactattttt ttgtagaaat
    10201 gtatctggca tctagaaaga gcaaatcagc ctactgactt actgtcaaag gataagtttg
    10261 aggaaagtct acacggagat agagacaaca acacagcata ttcaaaagaa acagaagcgt
    10321 gatgaccgtt gtcacccttc tgtttctttt gctatgtagt taaacaaagt agttgacact
    10381 tccgctactt agtattagta cgaaagtata cttcatgata ctaagtagat aagaagtgaa
    10441 aagcggaacc cacgatcgtc aaaatgctaa ctgctttgct tacaccagat aaagggaaga
    10501 gcaatatcgc ttattttagg cgtggagaag caagcaaatc cctacgatca caaaaaaaat
    10561 gctgacccag cagttaagag agttagaaca agatgaaagt atccatcgaa aagcctataa
    10621 ccaagtcccg cctaaagtag agtactcgct caccgaagaa ggaaaatcat taagagagat
    10681 tctagtagcg ttgtctgttt gggaagaaga acgtatcgaa aaacaaaaag cctcaaggga
    10741 agaagacacg ctactgcata cacatggtgg ctacctaaat tattgatttt ctatgacatt
    10801 tattttaaat aaaagaaaac aggagttttt ttaaaagcat catctatttt ttatgtaaaa
    10861 aaatggtatg attaatcaag taagaagtga ttggaagaag gcaagcaaat gaatagtatg
    10921 gaaaaagtca accagtttag agatgaacgt aattggcgac aatttcataa cgaaaaagat
    10981 ttggctatct ctatttcttt ggaagcatct gaattgttag aactttttca atggaaaacg
    11041 ccggaagaag taaaagaaac acaattagaa cgaatcaaag aagaactggc agatgtattg
    11101 atctattcat acatgatggc agacaacctt cacctggatc tagatgagat cattgaagaa
    11161 aaattgatca aaaataattt gaaatatcct gtagaaaaaa gcgcaggtgt acgaaaaaaa
    11221 tacacagagt tgtagaaatg tgtcattcgg ctgttggaaa gagcaaaaga aaaacagtaa
    11281 aatgagtctg gagggagaga aaatgaaaaa aaaaggaaaa gcaaaaagat ggctagtcaa
    11341 tatcctcttg ttcctcttgt tattagtagg actagctttg attttcaatg aacagatcaa
    11401 ggattatttt gtcagagaga caggagataa atatgctata gcgaatgtaa caaaagaaga
    11461 tctgaaaaag aataacgata aagatgtcag ttttgacttt gatgcggtag aaccaatgac
    11521 gacagaaggg gtcatgaggt cgcaaatgag aggaacagat ctaccagtga ttgctagtat
    11581 cgcagtccct tcagtttcaa tcaatttacc ggttttcaaa ggcttggata atacgtcact
    11641 actttacgga gcaggaacat tatctccaga tcaagaaatg ggaaaaggaa attatgcctt
    11701 agcaagccat cgtgcaacga accctgaatt gctgtttaca ccgctagaaa atttagagat
    11761 gggtgccaag atctatttga cagatttaga aaatgtgtat acatacaaaa cattttttaa
    11821 agaaaaagtt gctcctacag atacccaatt actgaatgaa gtcgagggaa aagaaattgt
    11881 cacactaatt acttgtggcg acatggacgc cgtcacgcga ctagttgtac aaggagaact
    11941 ggaaagtgtt acatcgataa aagatgcgac agatgatatg agatctgctt tcaatctaga
    12001 gacaaaaaca ttttaggata ctgggacata agtcgttaca gattaaaaac agccaaagga
    12061 tatgaatcga taaggaaaaa ccaatttgct gaaaaaaaga gaaacgcaaa gaggtgcatc
    12121 tctgatttgg ttgttttcta gatagacttg tgttccagtt tttttatttt ttatatgaaa
    12181 aggcttgcat aaataaaaat tcgatggtat gatgattttg aataagaaat ggattgttac
    12241 tgtacggcag gcaaaaccag aattcagcta agaaacagta ggcgaattct aggttttttt
    12301 atttttcttg agagggcgaa gcaaaatgaa aattgataaa attttgaata acaatgtcgt
    12361 catttcgaaa aatggattcg gggaagaagt cgtttgtatg ggaaaaggac tagcttttca
    12421 aaaaaagatt ggggacgaga tttcgccaga agcggtccaa aaagaatttg ttttaagaga
    12481 ttcttttgct aaaaaccaat tccagcagct gatggctgat attccggcag aggagatcga
    12541 atgggtcaaa caagtagtcg aactggctga agataaacta aaagttgaat tttcgcctaa
    12601 tatttatctg acattgaccg atcatcttca ttatgcgatc acacgagcaa aagaaaatat
    12661 ccaactgcct aatccattat tatttgaaac aaaaaaattt tatccgaaag aatatcaagc
    12721 agcgaaagag gctcttttgt tcgtgaaaca aaagacgggt gtggaacttt ctgaggatga
    12781 agccggtttt attgcctttc attttgtgaa tagccaacaa ggaaacgaag acatgcaagt
    12841 gacgatgacc gctaccacaa tggtaagaga tatactgagt atcatcagta aattcttcgg
    12901 catcgtgttc gatgaagaat ctttgaatta tcagcgtatc attactcatc tgcagttttt
    12961 tgcccagcgt tatctcaaag gggaaggatc tgacgagcaa gatgaatttc tctacgcatt
    13021 agttcaagga aagtatccaa aagcgtttca ttgtgtcgaa cggatcaatg agtatcttcg
    13081 acaaacgcaa aaacaggaaa tggggatcgc agagcagatt tatttaacga ttcatattca
    13141 aagagtagta agtgagaaaa aaagcatcca atcataagga ttgttactgt atggcaggca
    13201 aaacctggat tgaaaaaata ggttcacgaa tgtttgtgac tgtttttttc aatctgggtt
    13261 tttatttttt aaataaaaaa gaaagaagga aaaaacaatg gataatcaag cagttggtcg
    13321 tcgtgtatgg gaagcggtag gaggacaaaa gaatgtcaaa agtttagtac attgtgctac
    13381 gcggttgcga ttcagattaa aagatgaatc tttagcagat acgcaaaagt taaaagaaga
    13441 cccggacgtg atccaagtcg tacaaagtgg cggtcagtat caagtagtga ttggcagtaa
    13501 tgtagcagat gtttatcaat cgatcgtgga tgaggaaggt cttgcagttt cggaggatgg
    13561 aaaagaagag tccaaaaata ttttgaatcg tctgatcgat attatctcta gtatatttac
    13621 accttttcta ggggcgatgg cagccgccgg ggtattgaaa ggattcttat cattggcaac
    13681 ggtgatgggc tggctgacag ctgatagcgg tacttatcaa atattgtttg ctgcagcaga
    13741 tggtgtcttt accttcttac ctgtcatgct tgcttttaca gcagctaaaa agttcaaagc
    13801 caaccagttt ttagcagtag caattgctat ggcacttgtg tatccagcta tcacagcagt
    13861 tgctggtgcg ggagaagcta tcagtttctt tgggatccca gtgattcttt caccatctgg
    13921 ttatacttct tctgtcatac caatcattct agccgtatgg gttcaaagta aattagagcc
    13981 attcgtaaaa aaagtgattc cgcaatttct gcaaatgatt ttagttccgc ttgtcgtttt
    14041 agtagtgatg gttccattaa catttcttgc tttaggacca atcgggacgg ttgccggtaa
    14101 tgctttaggc ggtctcttca actcgattta tggatttagt ccaatcgtag caggtctaat
    14161 catgggaagt ttatggcaag tattcgtgat gttcggcatg cattggggat tcgtaccgat
    14221 catgtttttg aatatcgaac aatatgggtt tgatgtattg atgccaatgc ttcttccagc
    14281 catcttggca caaggtgggg ctgctttagc tgtagcattg cgaacaaaag atacgaaact
    14341 tcgagcatta gggatttctt ctacagtgac ttcgttattt ggaatcactg aaccaactgt
    14401 gtatggtgtg acattacctt tgaagaaacc attcattgcc gcttgtattt ctggaggtat
    14461 tggtggagct attatcggat tttccggagt aaaagctttc tccagcagtt tggtcagctt
    14521 attgacgatt ccgactttca ttaatacggt ggatggtgta gagtcaaatg tgacagtagc
    14581 agtaatagct acaggaattg cttttgttct tgcattcgta ggtacattga ttttaggatt
    14641 tgacgaacaa acacaagaca atcaattaga aaacaaacat gcgaatgcag gaaaaccgat
    14701 tacttcagca cggcatacct taaaaagtcc tttaacgggt aaagtactgc cattgagtga
    14761 agtaccagac caagtttttt cttctggcgt gatgggaaaa ggtatcgcta ttgatccaga
    14821 agtaggagaa ctagttgcac ctgcagacgg agagatcacg actatttttc caactggaca
    14881 tgctgtaggg atcacaacga ctgacggagc ggagattttg attcatatcg ggatggatac
    14941 agtagaatta aatgggaatg gctttgagat cttagtcaaa caaggggatc tcgtcaaagc
    15001 aggagattta ttgattcgtt ttgacatcga agcaattaga gcagctggct atagcgtaat
    15061 cactccagtt gtcattacga atactgatgc atttgccgat atacttgaat tagaccaaaa
    15121 agagattatt gcaaacgaag atgttctggc aattgtaaaa taaggaggaa atcaggatgc
    15181 aatcacgttt ttcagaaaaa tttttatggg gtggagcaac tgctgccaaa tcaattagaa
    15241 ggggcttatg atcgagacgg aaaaggacta tccgttgcag atgctatgcc tggaggtaag
    15301 cagcgctttg cgattatcgg cagtgaagaa tttgactgga cgattgatca agaaaaatat
    15361 atttacccga atcatcgagg aatcgatcat tacgatcgtt tcaaagaaga tcttgccttg
    15421 tttgcggaaa tgggattcaa atgttatcgt ttttcgattg cctggacacg aatttttcca
    15481 aatggagacg aaggtactcc taacgaagcg ggattagaat tttatgatca gctgattgat
    15541 gagtgcttaa aatatgatat tgaacctgtg atcacgattt ctcattacga aatgcctctt
    15601 catttggcaa aagaatacgg tggctggaaa aatcgaaaac tgatcgactt ctatgaacga
    15661 tttgctcaga cagtattaga gagatacagc agtaaagtca aatattggat gactttcaat
    15721 gaaatcaatt cggcctttca ctttccagct ttaagtcaag gattagtaaa aagcaatgga
    15781 gcaggagagt accaaaatat tttccaagca tggcataatc aattcgttgc aagtagcaaa
    15841 gccgtaaaaa tcggccacga attgcgttcg gatatccaaa tcggctgcat gattatctat
    15901 gcaacaactt acagcatcga tgccaatcca gtcaatcagg cagcaacaat gatccaaaac
    15961 caagaattca attttttctg cacggatgtc caagtacgtg gagaataccc tgcatacaca
    16021 gcgcgtaccc ataaaaaata tggagtcgat ccggaaaaat tagagcaaac ggaaaaagac
    16081 ttccagttgc taaaagaata tccagtggac tacatcggat ttagttatta tatgtctacg
    16141 gcaatcaatg agactgatcc acaagcagca acttcagaag ggaacttgct gggaggagta
    16201 aaaaatccgt ttcttgaggc aagtgaatgg ggctggcaga tcgatccaga agggctgaga
    16261 atcgcattga atgaattata caatcgttac cagaaaccat tattcattgt tgaaaacggt
    16321 cttggagcca ttgatcatat ggaagaggat ggcacgatta ttgatgatta tcggatcgat
    16381 tatttacgtc gtcatatcga agcgatggct aatgcagtag cagacggtgt agatttgatg
    16441 ggctatacgc cttggggatg tatcgatctt gtcagtgcat caacaggaga aatgagtaaa
    16501 cgttacggct ttatctatgt ggatctagac gatgaaggaa atgggacact gaatcgatca
    16561 aagaaaaaat cttttgattg gtataaagaa gtaattgcct caaatggcgt aaacttgtaa
    16621 ttaaagcagc tgaatgaaaa agaacacact tctcacatgt gatttgtgtt ctttttttat
    16681 tttttatgtg ctattctttt agtaatagta aagcaaaggg tgacgttatg aaaaggacac
    16741 gtgtactata cttggaagtc gcagataaaa ttaaagaaga tattttctca ggaaaatatc
    16801 cagtcggttc gatgttgccg acagaaagtg aattagaaga actgtttcaa gtcagcaaaa
    16861 tcactgtccg aaaggcaatc gaactgctgg catcagatga atatgtcgca aagaaaagtg
    16921 gtcgtgggac gacggttcta agcaatcgtc cttacaatcg attatccaaa gcggcaagct
    16981 ttacgcaaat cttggagaac tcccgatatg aagtccggaa agaaacgctg gatttgcaat
    17041 tggtccatat aaagaaggaa gatcctttgt tttccttttt cggtgagcaa gctgtttgtt
    17101 ttaagcgctt atattatttg aacaatcagc cttatattta ttttgttcat tatcttccac
    17161 aagaaatgat gcaattcagc agagaagaat ttggcaaaga atctttgtat cgtttgctga
    17221 cgaaaaaagg atatactatt gactcatttt ctgatgattt ctcggcagta tttttaacag
    17281 aagaagaaca aggaattctg aaaacagacg cacagatcgg catcaaacgg atacggaaat
    17341 cgtctacgca aattggggaa atgattgaat attcggaggc ggtctaccat acggagctgc
    17401 atccttacca tattgattat gaaacataaa taaaaaaaga ggttgtgaaa aagctgccct
    17461 gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttcg tttggttagg
    17521 cttattaccg cagatacacg gctgtttctg gattaccatt tattctggat aagggggtgt
    17581 ggcacgattt tcagaaggaa tctatgaacc aatcactgta ttgattttag cttcgatggt
    17641 aaccccattc ggcttgacga tcgcttattt cttagggaaa atcattcgta aaaatatact
    17701 caatcgtcaa gaaatcgata cattgaagac cgcttttcct atggggatct gtcagatcac
    17761 agagggatgt ttccctatcg tattgaacga cttggcacgc aatgtcatag caacgggagt
    17821 cggtgggaca gtaggtggcg gattaagcat gttttggggc gcagatagcc gtattccggc
    17881 atcgggaatg tttgcagtcg ctacgatgac cagaccatgg gcattcattg gtgcattgtt
    17941 agctggttct tttgtcacag gaataaccat tttattattg aaaaaaccag tagatccgaa
    18001 tgcagaaatc atccaagaag aaaaagagga agaagacata tcttgggatg atttgacaat
    18061 tagttaggag aggcagaatg gattacaaga aaaaagtaca atagatgtac cttgagacag
    18121 gaatggtatg aaagaatcag agttagaaag aatcgaatat acagattttg gtttaggagc
    18181 gatcgaaact gtccagagca tcggcaccct ccttgaaatg acgatgaagg aaaaatgaga
    18241 acctttcgtt gtcgaaaggg tctggtatac cttatgtcga aggaaaggaa acaaaaaaac
    18301 tttcctgtac tattccaaaa aggaaagaaa gttattatac agtaaaaaaa gaaattattc
    18361 ttcatgctag agatcagtac acgttcgagc ccaatatcaa acactagttc caggcaggcg
    18421 caacaggtgc ggttatcact gaattttctt ccagcagtga cgattcaagt gatattttta
    18481 caaatccagc tatttatcgt taacaaaaag atttttagga gaagctgatg aacaaaaatg
    18541 atcaaatttt gatcgtttag ttcgtcggct tttttattta gagaaacatc atttatcagt
    18601 gaaacataac acgagaacaa aatgattttt tcgttaataa aagaaacaag tatttgttgt
    18661 ttaaaataat acacgttatt ggcatatagg acgaaatccc agtatcttct ataagtaagt
    18721 catttcatat cgatgaaaac acgttattta tttttgtttt atctataaaa tggct
//
LOCUS       DAYYDS010000052        18740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000052 DAYYDS010000000
VERSION     DAYYDS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.052"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..531
                     /locus_tag="ACHA02_001802"
     CDS             256..531
                     /locus_tag="ACHA02_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670124.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="ACHA02_001803"
     CDS             544..2616
                     /locus_tag="ACHA02_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8670125.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2784..4667
                     /locus_tag="ACHA02_001804"
     CDS             2784..4667
                     /locus_tag="ACHA02_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8670126.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4679..5626
                     /locus_tag="ACHA02_001805"
     CDS             4679..5626
                     /locus_tag="ACHA02_001805"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8670127.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="ACHA02_001806"
     CDS             5646..6173
                     /locus_tag="ACHA02_001806"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8670128.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="ACHA02_001807"
     CDS             complement(6236..6889)
                     /locus_tag="ACHA02_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8670129.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="ACHA02_001808"
     CDS             7021..7863
                     /locus_tag="ACHA02_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8670130.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="ACHA02_001809"
     CDS             8021..8917
                     /locus_tag="ACHA02_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8670131.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="ACHA02_001810"
     CDS             8920..9633
                     /locus_tag="ACHA02_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8670132.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="ACHA02_001811"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="ACHA02_001811"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8670133.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="ACHA02_001812"
     CDS             10168..10395
                     /locus_tag="ACHA02_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8670134.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="ACHA02_001813"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="ACHA02_001813"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8670135.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="ACHA02_001814"
     CDS             complement(11181..11723)
                     /locus_tag="ACHA02_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8670136.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="ACHA02_001815"
     CDS             complement(11960..12358)
                     /locus_tag="ACHA02_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8670137.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="ACHA02_001816"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="ACHA02_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8670138.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="ACHA02_001817"
     CDS             13471..14238
                     /locus_tag="ACHA02_001817"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8670139.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="ACHA02_001818"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="ACHA02_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8670140.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="ACHA02_001819"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="ACHA02_001819"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8670141.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="ACHA02_001820"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="ACHA02_001820"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8670142.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DAYYDS010000053        18184 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000053 DAYYDS010000000
VERSION     DAYYDS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.053"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(402..905)
                     /locus_tag="ACHA02_001821"
     CDS             complement(402..905)
                     /locus_tag="ACHA02_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8670143.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1264..2289
                     /locus_tag="ACHA02_001822"
     CDS             1264..2289
                     /locus_tag="ACHA02_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8670144.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2612..3490
                     /locus_tag="ACHA02_001823"
     CDS             2612..3490
                     /locus_tag="ACHA02_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFM8670145.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3966..4082
                     /locus_tag="ACHA02_001824"
     CDS             3966..4082
                     /locus_tag="ACHA02_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8670146.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5010..5348
                     /locus_tag="ACHA02_001825"
     CDS             5010..5348
                     /locus_tag="ACHA02_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670147.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5442..5672
                     /locus_tag="ACHA02_001826"
     CDS             5442..5672
                     /locus_tag="ACHA02_001826"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8670148.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6072..6443
                     /locus_tag="ACHA02_001827"
     CDS             6072..6443
                     /locus_tag="ACHA02_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8670149.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6717..7409)
                     /locus_tag="ACHA02_001828"
     CDS             complement(6717..7409)
                     /locus_tag="ACHA02_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8670150.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7412..8197)
                     /locus_tag="ACHA02_001829"
     CDS             complement(7412..8197)
                     /locus_tag="ACHA02_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8670151.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8434..9834
                     /gene="lacG"
                     /locus_tag="ACHA02_001830"
     CDS             8434..9834
                     /gene="lacG"
                     /locus_tag="ACHA02_001830"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8670152.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9859..10176
                     /locus_tag="ACHA02_001831"
     CDS             9859..10176
                     /locus_tag="ACHA02_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8670153.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10189..11862
                     /locus_tag="ACHA02_001832"
     CDS             10189..11862
                     /locus_tag="ACHA02_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8670154.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11876..13507
                     /locus_tag="ACHA02_001833"
     CDS             11876..13507
                     /locus_tag="ACHA02_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8670155.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            14379..15569
                     /locus_tag="ACHA02_001834"
     CDS             14379..15569
                     /locus_tag="ACHA02_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFM8670156.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            15703..17892
                     /locus_tag="ACHA02_001835"
     CDS             15703..17892
                     /locus_tag="ACHA02_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8670157.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            17889..>18184
                     /locus_tag="ACHA02_001836"
     CDS             17889..>18184
                     /locus_tag="ACHA02_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8670158.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEF"
BASE COUNT         5934 a         2926 c         3640 g         5684 t
ORIGIN      
        1 tcaaaaagta ggatcaggca gaatatactg gcagaatcaa ggagaaggga gcctaaataa
       61 ggagaaaaac catagaaaat ataaagtaat tacttataaa acaagctttt tatagaaaaa
      121 taatatacct gagtagggta taaattaaga cggaattcct ttctagattg agatattgac
      181 taggaagaat gttaagattt taagccttta agctaaaaaa aaaccctttt tgttaaataa
      241 ggggaatata gcctgtcagt ttcaaactgc aagtcgtcct gctgcaataa tatagtagat
      301 tagagatgaa aatagatttt tttaaaccaa tgactgaata aaaagaccgt catagataca
      361 cattggaatg tgtctattac ggtctttttt tacgaacgat tttattgaag acggacagtt
      421 cgggtcaaaa ataaaaaagc tacgatcaga aataagaata aaatgtatcc caaaataatg
      481 gctagacggt ctaacttatt tgtcgataga tgtttgttta gaagttgata ggcccataaa
      541 cctatcagtc ctcctgttgt attagttatc aaatcagtga tgtccgctac gccaataccg
      601 aataggaatt gaatcaattc tgccaagaag cttacaagga aaataatccc taatttttta
      661 gaaaaagaaa gtctcttgaa attgacattc aaaagcaccc caaaaggaaa aaagacaatc
      721 aagttataag tggtctcacg taataccgtc gtattttcaa aagaaaaagt agaaaaaggg
      781 acgagattga cattgctgta agaataagtg agaaattgtg gaagattaaa tgatagcttg
      841 aataaaatca accataatag aaagaataaa taaatcccta ataaaaattt tgatacatac
      901 cgcatagaca tctcctccat ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa
      961 aaagtatgaa gtttaaggac caacgtagtt atcgtacgaa aattagaagc ttttttttgt
     1021 tgggaatccg gtttctttaa caaagctaag atagcgagtt gcttggaaaa gttgctatct
     1081 tttttctgtc aaaacgactc ggaatgttcg ttttttgttc taatttctcc aagttgttta
     1141 ctttttgata ctaagtttat gaaaaatcaa gaaatagttg ttatactagg taatgctgac
     1201 aaacgggatt ggcatttcat cttggttcag ctttttttcc aattactagg aaggagggat
     1261 agattgttca agacttattt tcgtttcaat aaaatacaag ataatccttt tcgtatcatg
     1321 aatctgatgc tacttatctt tattgtatta tggttaggtt acataaatca tgattttttg
     1381 atttcaagtt tttcatcatt agggatcttt acttttttct attatcaaaa cataccgatg
     1441 aaatatttgc tgaaacgtat gtttctgatc ggttgcggac tgttggttgc tttcgttttg
     1501 gggatcttat cgacttatgt tttatggctt gaaccatttg ccgtagcttt ggttgctttt
     1561 tccagcagat tcgtgttgcg attattccat atttctaaac caggtggctt gttttttgcg
     1621 atgctttctg ccatgggaac gagcatgcag ctgccaatcg ctcaattacc gatcgtctca
     1681 ctttattttt ttatgggtgt cgtttttgcc ctgattgcgg cagtgattac aaaattactg
     1741 gattcacggc ctgagcagac aattgaaaaa gcaactttaa aagaaagatt tcacgaagag
     1801 cctttagtga tcattgattc tgttttctac agcgctgcct tatttctttc cgtttatgtc
     1861 agtcacggct tgaatctgca taatccttat tggttgacct tgtcatgtgc atcgattttg
     1921 ctagcagaaa acttggatgc gatgaagcat cgacaagtcc aatatctgat tggatcgatg
     1981 ggtgggttat gtgtgtcggc attcttgtca tttgtgccgt ttacgcagtt gcagaccatt
     2041 tttctgatca cttttctata tggaatcgca caatttctag ttgctagaaa ttatgcagtt
     2101 gctaatattt ttcttaatcc gatggcgttg atgctatcta cacttattcg tggagcatac
     2161 ttgatttctt tgatcgaata tcgtttttta gggatcgtca taggaagttt catcggttta
     2221 ggtgttgcgt gggtaatgac ggttggtttg caacattatt taacggttgt tcgtcaaaaa
     2281 ttagaataga aggacaaata tatgtgccaa aagaggttat gacaagattt ttgtcacaac
     2341 ctctaattca aggtgttcaa aagccagatt ctcagcatta agtcaaattc acacaaaaat
     2401 gtggaaagga actttcaaaa aaattttata tttattctat caaaaaattt tgtttatgat
     2461 ggtaactgaa taaactttcc tgagatggtc aatgctttcc ttgaaagtct tacagatatt
     2521 ctttcttcat agaaagttta agttttatag ttagtgtaca ataaaagtat gtttatacca
     2581 tatggtgtcc ggataatcag tcatttaaac aatgaggttc aaagatatga aaaggggaaa
     2641 tttgatgaag aaaataagca atgaaatcca aattctggca gaaactttaa ctcttcccaa
     2701 agtcgttata gagcaaatga ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca
     2761 atatagtcat gaactaatgg atcctaataa agcaaaggcg actgcaaaga aaatagacaa
     2821 agtctatgaa gaggaacctt ttgcagcatt agctatttat ctatatgcag cttctcaaag
     2881 ttggaaaaaa atttatcaac cattgaagat tccaagaact atctatttgg caacaatgaa
     2941 tgcttttact agatttattc atgaacattt ccaagtaaca ggaagctatc gctttgaccg
     3001 tggtttttgg atttggcatt acactagtgg cttgatcttt cggattggtg aattggagta
     3061 cgaaatgaca tattttccga aagggtacac ggatctaaga ttagaaggaa agcagtgcct
     3121 ttctcttcat attccttccg atgcagattt atctcagaat aagataagag atagttacga
     3181 aagtgccatt cgtttttttg aactttattt tcctgattac cattaccaag tgatgtacac
     3241 agatacttgg ttactatcac ccaatcttac taaatggttg aaaaaagagt caaaaatctg
     3301 tctttttgca gccgattatc gattattatc agttgatgaa caggacgata gtggagtccc
     3361 ttggatcttt ggacgagtag atgcccaaat acatgattat ccagaaagta cgagtttaca
     3421 gcgacaagca aaagagcaat tgcttgcagg cgagcacatt ggctctggct taggtattct
     3481 tacttcataa gtttagtact tcgaaatagt ttaaaagagc gtgagacaaa agtaaagaac
     3541 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcggaa
     3601 ataagctgaa attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg
     3661 gagttaaacg cttctgtccc agcctctttt ttagtgttct atcgaacaca cagtacgttc
     3721 aataggtaaa ctagctataa caatagatat taaagttaac ggacaagagg gcctttttta
     3781 ggtagctaaa acgtaataat gaaaatttat ttccagagat aggagtcaat cagctagtct
     3841 gatcgactgt tttaacaagt tgactttcat tttaactttt gataccattt aaaacacaaa
     3901 tcgtaattgt ttcgatttgt gttttttagc atgactacaa tcaaagaaga aagtgggtcg
     3961 tccatatgga aaaaacagat atctcaagtg cataccgccg cttgaaaagc ccaaatatca
     4021 aaacacgaaa acgtgcgctg aagatcatca aagatgtgaa acggaatagt gggaaaaggt
     4081 aagaagaagg aaacttaatt aaaaaaatag ccgaaaaacg tttttcggct attttcaata
     4141 atcggttttt gtgtaaggtt tccgaaagaa ctgctgttga ttttccattt ttaacatatg
     4201 ataaattggt agaatctttc ttttcaaatt gataccattc cctaattcca gttatatagt
     4261 taactacaga accacaaagc acgtacgttt ataaaagtat tatctgtagt tatttttcaa
     4321 ttccctttca acctcttatt ttgaccagta gtattgatca ctttaccttt taacaccttt
     4381 gttcgttttt gaaacatttt tagtcaagaa tggaaacgta actacataat caaaggtttt
     4441 gcgaaatatt tcatgaaata tttcatgtag aagcgagcaa tacggttgca aataaccatt
     4501 atcgtttaat aagcagttac gaaagctccc aatatctcta acgttcagct cactccattt
     4561 cttttcttca aaaaaatcag tgattcgtat ctctttctat ttgtcgaaaa agttaacttt
     4621 aaaaagtaac gccgaaaaaa tatgcaaaaa aataaaaaag cagttactat tgctagaata
     4681 acatcaagga ataataagtt ttggttttca gataatagtc gataagttgt gtattggaat
     4741 tttagataca gtaataaagt atttttcatg aaattttgtg agaatcacta ttacaagtat
     4801 ctttaataaa attaatggtt aaggaggaac ttaaagtaga atgtaaatga atagttgtag
     4861 tcagtcgatt gtgatactag actcaaagtt gcaggaataa gtttttcaat ttgtgattgg
     4921 aagataaaaa atgaaaaaga agggcttttt tttatacttt gttgattgta gtgtttattt
     4981 tccctctgtt tagccaagta acattttcta tgactcaacc agtttcaagt gaggaacatg
     5041 ataacctttt tcgtgctaca gaagagttta agaaatatca ggaaaatatt gttccggaga
     5101 ctccattagc tagtgagcca attactgata ttgatggaaa ccaaatagga tggtatatac
     5161 agtacgaagt tatggtggta aataatgact caatagtaaa aaatacagta ggcttagagc
     5221 taacaccgat tgaaaaggtg agttcattgt taacgtatat ttatactgat gaaagtgacg
     5281 agactgagat cattttaacg gattactcaa aaattgctac tgataaaaag tatatattgt
     5341 ggatgtagac actagtgaaa tactttatga tatagaaatt gatgaaaatt tactagatgt
     5401 agcacaagaa atcgatcatc aaaagcagga agctattcga tatgctggta gaaaagggaa
     5461 aagaaaatat ttattacgtg aatgtagcaa aagttagaga agatgaaaat gagtggaaag
     5521 aattcaagag tcgctactca attaactcga ctccgacatt tacagtttac agagaaggat
     5581 cgatagaaaa aactgttttt tggactaaag aaagtggaat gtctttagct gaagtagaag
     5641 agtttttgga ttatgttagt atgcaacagt aagggagttc ttattatgaa aaattttgac
     5701 acatattatg ttattgtttt ttcgatatct ttgctgttaa tattactaac tttaatgcag
     5761 tataaagagt atccagagta ttttatttta acttttgtat atttagtaag tagctacaca
     5821 cttctatata aattcagaag ttcttctaga gtttctaaag tttgtttctt aattagctta
     5881 ttgattctat aatatttttg atttatatag gaaattaaaa atattataga atcaacactc
     5941 agagaacggg ttggttttta atagatttaa taagtactga taatgaaatt tctcctggtt
     6001 gggaaaacga agttggttac cctgtagaag aattttttca agattacttt gcggagtatg
     6061 ccgataggat aatgagagat tacgtaacag ataaaactca agaagtcatc aatcaatccc
     6121 agtcattaaa tgaattatct gataaggcga aagaagaagc agttgccatt atttcttcag
     6181 ggattagtga ctatattgat atgagtggtg aaatcaatta tggcgataaa gacaaaattg
     6241 tacgcatgat aaaagctttt atcaaaactt ttgatgtgaa ggcagatctt aagtttaatg
     6301 atacttacct agtaggaaaa ttaattaata tccttgggac agagaaagat acggaaacgt
     6361 ttatttcctc tttgtctatg ttgtttacag ataagcattt ttcaactcag cttggtggag
     6421 aggaactagt ggaaatcttt taaccattac gcccagcatt aataaaagta tatcaaaaaa
     6481 gtactcctga tgatttttaa gattggtttg agaaataatg tgaaaacact atttttaatg
     6541 ttaattatag atagagatga acgcgtcata tggttaaaag aatgaaaaag tcaaagatac
     6601 actgctatat ttcactaagg atatagcagt gtatctttca tgtcgtttca ctggcagttt
     6661 tttataaaag gaggtgtgca tttcagttgc ttttctttgg tagttagttg aatcgtttaa
     6721 gaattaacgg atgtggcaga caggtgctct gtccaaaaac cagtaaaatc tttgatggtt
     6781 ccatctttat ttagctgtgc ctgttcaaat actgttttgt ttttaccgaa tggatcaatt
     6841 gcaataattg ttccgatccc cgcacgtttg gcagcaagta atcctgagta agcatcttcg
     6901 ataacaagac aatcagccgg agctaagctc agtttgtctg ccgctttaag aaaaatatcc
     6961 ggttgcggtt tcccagggaa agagccgtcg tcatagacaa tcttgtccca gtcaaaccag
     7021 cgggctaaat cgaaaatatc aaaataaaaa gcaacattct ctttgacagt agccgtggca
     7081 atcgttaatg gaatctgttg gtcagtcagc tggtcaagag tcgcttcaag cccttttgtc
     7141 aactgaagct cgttttcatt ttgcaagcaa agcatacgat aatagctttc tttttcttct
     7201 gacaatacgg cgatctcaga ggcggtcaaa tgctctgaaa tgaaatgttt caaaatctcc
     7261 gtattcgtcc gaccatgaat attttttaaa atatcctcgt ctgataaacc atcttttgta
     7321 tgatcatgta tcatatgcag ccaagcagct tcatgcagat gagagtccaa aaacattgtt
     7381 ccgttgaaat caaatataat ccctttcata gttacgctcc tatagtggta ttttcggttg
     7441 aatcagcaat ttgcagatcg acatattgtg catagtgctc ttgaacctcc cgcgagatct
     7501 ggcgatccgt aatgagcaga tcgaaatcat gcaaattata aaacacataa aaatcatctc
     7561 gattgaattt tttattatca acgagtagat atttcttacg ggaattattt agagcgatct
     7621 gctgtgcttc tccttcttcg gtactgtaag tagagatcgc ttcgttgtga acgccatttg
     7681 aactgataaa tgcttttgta aactttagtc ttcttaaatt gtcgttggtg atcggtccga
     7741 caaatgcgcc ggtatttttc cggaaatcac cgccaatcag tagaatttct ggtccatctg
     7801 cttgagcaag aatatcaaaa actggataat tatttgtaat gatccgaatt ttcttgttca
     7861 gtaaatgcgt cgccaatagt tcgattgttg tacctggtcc taagaaaatc gtttctccct
     7921 cgtgaatcaa ggaggctgct gtggcagcaa tttctttttt ttcttgaatc tgcagagtgg
     7981 ctttttcagt gtgagaaagt tcttgatcta aagtgtagga aagactttga gcccctccat
     8041 ggacacgcag cagcttgccc gatttttcta attcatctaa atctctgcga acggtcatat
     8101 cagaaacatc taattgattg atgatttcat taacggtaag gattcctttt ttattgacca
     8161 tttctacgat agtaagcaat ctttcttttt tcaacattgt cttcacctct aatttaagga
     8221 taatctatca tataaacgga caacagtcaa tctaattcga acaaaagtaa acatttaggg
     8281 tggtttttat gtttgttaca atagatttta ttgctgtttt ctggttttta tgattatttg
     8341 tgtttgtttt tgttgacaaa tgttttggat gcgtttacaa tgcaatcagt tagaaagagc
     8401 gaaaactgat tatagatttg aggagagatg tacatgctta aattaccaga agattttatt
     8461 tttggcggag ccacagcagc ctatcaggtt gaaggagcca ctaaggaagg tggcaaaggc
     8521 gctgttgctt gggatgactt tttagaggaa caagggcgat tcagtcccga tccggcaagt
     8581 gatttttatc atcaatatgc aaaggacatt gaattgtgtg aacgttttgg tgtaaatggt
     8641 ctaagattat ccattgcttg gagccgcatt tttcctgatg gagctgggaa accaaatcca
     8701 gaaggaatcg ctttttatca tcgtgtgttc gaagaatgta agaaaagaaa cgtaacgccg
     8761 tttgtgacgt tgcatcattt tgatacccct aaacgtttgt ttgaccatgg agattttttg
     8821 aatcgagaaa caatagaagc atttgtttcc tatgccattt tttgttttca tgaattcaaa
     8881 gaagtcaagg tgtggagtac ctttaatgaa atctatccag ttgcaaccaa tcaatatttg
     8941 ttaggtgttt tcccaccagg aatcaaatat gattttacaa agatcgtcgc ctgtcttcat
     9001 aatatgatgg ttgctcatgc ccgtgtagtg aactatttca aagaaaacga acttccgggt
     9061 gaaattggtg tagttcactc attagagacg aaatatgcgg caacagacgc gccggaagat
     9121 aagcatgctg cctttttaga cgatgcattg tcgatccgtt tcttattaga tgctacctat
     9181 ttaggctatt attcaactga aaccttgact gctcttgatg agatatgtga agcaaatcag
     9241 gccagctatc attttcctga agaagatttc gttgaattga aaaaagcatc cacgcgtaat
     9301 gactacttag ggatcaatca ctaccaatgc cattttgtca aagcttacga tggagaaaat
     9361 gcaatccatc ataacggtac gggtgaaaaa gggacctctg tttataaagt gaagggaatc
     9421 ggagaacgta tttataaaga aggaattcct cgaactgatt gggactggct gatttatcca
     9481 gaaggtctat atgacttgtt gctgcgaatt aaaagcgatt acccacatta caacaaaatc
     9541 tatatcactg agaacgggat ggggtataaa gatcagtttg aagatggcat tattatggat
     9601 cagccgagaa tcgactattt gagagtctat ttggaatcct taagcaaggc gataacagcc
     9661 ggggtaaatg tgaaggggta ttttttgtgg tcgcttatgg atcttttctc atggacaaat
     9721 gggtataaca aaaggtatgg cttgttttat gttgattttg aaacccagaa gcgttaccca
     9781 aaagaatcag cttactggta caagttggtc agtgaaacca aaacgattat ttaataagtg
     9841 ataatggagg aagaaaagat gaataaagag gaattacaaa tgcttggttt tgagatcgtt
     9901 gcatattcag gcgacgctcg cagtacatta ttgaaattat tgaaagaagt aagacaagga
     9961 aactttgaac atgttgattc ggcgttgaaa gaagccgacg aaaatttaac ccttgcacat
    10021 aattcgcaaa caaaaatttt agcggaagaa gcatctggga aagagatgga gatgggattc
    10081 atttttatcc atgggcagga tcatttaatg accactttat tgctgagaga tctgattcaa
    10141 gactttattg tactgtacag acagaatcaa ggataggaga agaacgctat ggatggaatc
    10201 attaaacaaa tcgaaaaagg gaaaccattt tttgaaaaaa tctcacgtaa tagttattta
    10261 ggagccgttc gtgacggatt tttaacagcg atgccagcta ttttattttc aagcattttc
    10321 attttgattg ctgcggttcc tgagattttt ggttaccaat ggcctgaatc tgtctccaca
    10381 tggctatgga aagtctataa ttattcgatg ggtgttgtag ggttactcgt ttcagcaacc
    10441 actgctcgct gcttggctga ttcgatgaat cgtcgcttac cgaaaaacaa agtaatcaac
    10501 accacctctg tgatgctggc ctctgtggta agtttcctgt tgttaagcgt ctctccgatt
    10561 gaaggtggat tttcaacgga atatatgggg acaaagggac tactggcttc ctttgtcgct
    10621 gcatttttaa cagttaattt atataagttt tgtgtgctga aagatattac cattaaaatg
    10681 cctaaagaag ttcctggaac gatttcgcaa accttccgag atatttttcc tttctctttt
    10741 tcagtatttg cggccgtttt aattgattta ggcattcgac aaatcttcca aatctcattt
    10801 gcagaagcaa tcattacggt gctgcagccg ctatttacag cagctgacgg gtacttagga
    10861 atcgctatta tctggggagc gatggcgctg ttttggtttg tcggtgttca tggaccgtcg
    10921 atcgtggagc cggcaattgc ggcaattatc tatgccaatg tggaaaccaa tctgcaattg
    10981 ttcaattccg gtgaacaagc ttctaaagta ctgacggtgg gtttagggaa ttttgtagga
    11041 actatgggag gaactggggc aacattggtt gttcctttta tcttcctact ctttgctaag
    11101 tctaagcagt tgaaggcagt tgggaaagct tcctttattc cagtaagttt cgctgtcaac
    11161 gaaccattgc tatttgcggc acccatgatt ttgaaccctt atttttttat tccatttttg
    11221 ttaacacctg ttgtaaacgt ttgcctgttc aagttttttg tcgatgtatt gggcatgaac
    11281 agctttatgt acgttttgcc ttgggcaact ccagcaccga tcggtttggt tctaggaacg
    11341 ggcatgggta tcttagcttt tgttctagca gcggttctaa tcgtcgttga cttcctgatc
    11401 tatctaccat tttgcaaagc atacgatgct gtcttagtag agaaagaagc acaaaaagca
    11461 gaggtgatcg caaccgagga aggtgcttct gccgatgtac gtgaggaagt acctgtggca
    11521 gcgagtcaag ttatcagcga tgcaaataaa gaaatcaatg tgttggtact ttgtgctggc
    11581 gcagggacaa gtgcgatgtt agctaatgct ttaaaaaaag gtgcatctga atcgaaagca
    11641 ccgatcagtg caatggcggg tgcttatggt tctcattacg aaatgctgca ggattttgat
    11701 atggtggtct tagcaccgca agtcaattct tactatgatg atatcaaaga agacaccgat
    11761 cgcttaggaa tcaagctggt tgcgacaaaa ggagcagaat atatcggatt aacgagagat
    11821 cctgaaaaag ctgttcagtt tattctgaac caatttcaat aaaggaggag ccataatgaa
    11881 aatgggaatt tggttgcgta aagctgtttt atttgcagct gtgttagggg ctttgggggc
    11941 tgtttcaaaa actgttgaag catctgccag cgaatttatt cgcggagcag atatttcgat
    12001 cctgtcagat atggaaaaat ctggtgcaaa atattatgaa gacgatattt caaaagatgc
    12061 cttagagatt ttaaaaaata atggtgtcaa ctatgttcga ctgcgtttgt ggcaggatcc
    12121 ttataatagt aatggagaaa gttatggagc agggacaaat gatctggaaa cgacgattga
    12181 ccttgcaaaa cgagcaaaaa atctgggact taaggtgctg ctggattttc actacagtga
    12241 tttttgggtt gatcctggga aacagaatct tcctaaagca tggcaaggtc tgacctttga
    12301 agaaatgaat acggcattgt acgactatac aaagaacgtt ctgtcggaaa tgaagcagct
    12361 ggacgtttat cctgatatgg ttcagatcgg caatgaactg aatagcggaa tgctctggcc
    12421 ttatggcaag tcttggggag aaggcggggg agaatttgat cgattggctg cttttttgaa
    12481 gtctggtatc caagctgttc gagacactca acctaaaaca actccagtaa tgctccactt
    12541 agctgatggt ggcgatacag gagcatttac ttggtggttt gatgaaatca cgagtcgagg
    12601 tgtgtcattt gatttaattg gagtgtcgta ttatccgtat tggcacggtt caatgaatga
    12661 acttcaaacc aatatgaatg cgatcagcca acggtatggt aaagaagtga tcgtagttga
    12721 aacagcttat gggcatacaa cagcgaatgc cgatactatg ccaaatgctt ttggtgaagc
    12781 cgaagcagct gcggggggat atgaaccaac accgacagga caagcagagt atttgctgga
    12841 tcttgccgat cggattcagg cagtaccgaa taatcgcggg gcgggcttct tctattggga
    12901 acctctttgg tacaacggaa atgtgagttg ggcaacacaa gcaggtatga attatctcag
    12961 tgttcaatcc actatgggaa acgaatggga caaccaagcg atgtttgatt ttgctggaaa
    13021 cgccttgcca agcttgcgtg cgtttaaaca agcaggggcg caaataaatc tagttaaaaa
    13081 tgcaagtttc gaaagcgatg gaccaacgac cagtccttcc aattggcaaa cctggtttca
    13141 acaaggaacc gctaagggta cgatcaaaac ggaagcgggc actttttttg gggactacaa
    13201 actaactttt tggcaggatc aagcatacga ggcatcagct tatcaaaatg ttagtggcat
    13261 aagcaaagga acttacacat tgagtgccta tgtaatgtct gggggagagt ttgatactaa
    13321 tcagttatat atcaaaaatt atggtggagc ggaactaaat actgcggttg ttaaaacaag
    13381 tgaatggaca aaaattacaa tcaaagacat tccaattaca acaggtgttt gtgaaattgg
    13441 ggtttatagc aaggcttcag gaaatgcttg gacgaacgtt gattttgttt cattggtgaa
    13501 acaatagact gcggatcgtt tcgattcaag atcggaggtt cccttcagcc acatcgttag
    13561 cgtagaaggc aggaatcgaa cctgcacctc gcctttgcca aaaataacca gaaacgattt
    13621 ccagtcattt ttaccaaaac gagtaaagca ccagacttca tctacacttt gtattatgct
    13681 aaagagagtt caacgatttc gtaagaagca atgaaaaaat tgcaaaaagt tgtgcagata
    13741 acataaagtt tgtttaagaa agggtaacct tagaattccg tcgtgaatat gatttgagca
    13801 cttagatgga tagaaaaaag ttctcggaaa acagttcgtt tttcgagaac ttctttttat
    13861 gataagaatg aaacaaatta acttatatta tagtctcttt tggtagggaa ccatcttatt
    13921 tttaatgaaa atcaagcatt ttagctattg aagaatgttg atttataggg attccccctc
    13981 catattatgt tggctcttta ttcccactct gttttcaacg taaagtgtat aaaacagaat
    14041 gcgatactat caagggagtt aaaacaatat acagaacagt aaaacactat aaataaaaat
    14101 aaaagtgcaa taaaagtgca atttttgcac ctaatcatac tctgtttata tatgaaaact
    14161 agattctgaa tccaatattt ggttaacgag aggcgttcga atttgaacgt cttttatata
    14221 ttactttcaa ataaatatga ggtagaatac tatctatttt ctttttaacc caattgttat
    14281 aatggagagt gtaaaatatt ttgtgtaaat agaaaaaagg aagtcccttc tgtagaatag
    14341 agttaccaca acacattcac agaagagagg acttccctat gaacgatttt actacagaaa
    14401 ttctaaagac tctagcgaac aaaggcgatt tgaatgaatt attccgtgtc catttggaga
    14461 aagcagtcaa tacgcttctc aaaacggagt taacggcttt cctagattac gaaaagtatg
    14521 atcgcattgg ttttaacacg ggtaattctc gtaacggctc ctatgaccgt acggtcaaga
    14581 ccgagtatgg ggaacttcat ctccagattc cgcgcgaccg caatggtgag ttcaagcaac
    14641 agactgttcc tgcttataga cggacgaatg acacgttaga ggagaccgtc attcacctct
    14701 tccgaaaagg tattaccatg tcggaaatcg cagacttgat tgagaaaatg tatgggcatt
    14761 actacacgcc ccaaaccatg tccaatataa caaaatcatt tacagaagag gtaacggcgt
    14821 ttaatgggcg ggagcttcat gaccgttatg ctgctattta tatggacgca acgtatattc
    14881 cgttaaagcg gaaaaccgtc gccaaggaag ctattcatat cgcagttggc attcgcccgg
    14941 acggatcaaa ggaagtattg agctatgcga ttgcaccgac tgaatccatc acgatttggg
    15001 aggaaatttt attggacctt caagagcgcg gtttgaaaaa tgtcctcctg ttcatcacgg
    15061 atggcttaaa ggggatggta ggagcgatta gtcggttcta tcccaaagct cgttttcaac
    15121 attgttgtgt acacgtttcc cgtaatatca gtcataaagt gcgtgtcgat gatcgtaagg
    15181 aagtctgtga tgattttaaa atggtgtatc aagcctcatc taaagaggtg gcgttggaag
    15241 cacgtggtac ttttgcggaa aaatggaaaa ccagctatcc aaaagtggtt gaatcgattc
    15301 tttcgaacga tcacttgctc actttctatg atttcccctt ggccatacgc aagagtattt
    15361 attctacgaa cttgattgaa tcctttaata aacaaatcaa gaaatacagc caccgcaagg
    15421 aacagttcca aaatgaagag tcgatggaac gtttcttagt ctcgtctttt gatacttaca
    15481 accaaaaatt cctaggtcgc agtcataaag gtttccaaca ggccgaaggc gagctcgaac
    15541 aaatgctaag ccaactgatt gagaattaga aacaatctgc agtagggaag aatcatttac
    15601 acaaaattat tgacgctccc ttataatgag aacttttact aacataattt atgataaact
    15661 tactatataa tcatattaat taaatagtta ggagatctgc ttatgtttat atctactctt
    15721 caaattcgaa attttagaaa ttatgaatcc gaaatctttc gattcgataa tgagacaaat
    15781 actattatag gtgagaatga ttcagggaaa accaatgctc ttactgcatt gagaatctta
    15841 ttagatgata gttattacta tagtagtaaa acactaaaag aatcagattt ttttcatgga
    15901 attcaaaatg ggtggcaagg gcattggatt attatctctg cgacatttga aggaatcagc
    15961 gaagaagaat ttgacaatga gatttgtgct tccatttctt taaattctga aagtcaaaca
    16021 atcttagaag aattgatttc aaatgcagat aaaggtgtag gtagtatttc tctatttatt
    16081 agacctaaca aagctattag aaagcaatta tttgatattt ccactgaatt agactctcat
    16141 caatttaacg aatttagaaa ttctattcgt ctatccgact atgaatttta ctacacatct
    16201 aaatcaaatc taaatttctg tatcaatgaa aattatgata aattggtagg aagactcaat
    16261 gacaatatag ctagtaatcc tgaagaagac gatgaagctc tgttgggctc aagaattgac
    16321 atgtcagata ttttcaagca tatttcggtt gtttatgttg acgctcttcg agatgttctt
    16381 agagaaatga aaaacaatcg aaacccagtc aaaagaatca tggaaacaat tgaatcaaaa
    16441 atatcatcag ataatgttga gtcattaaaa acaattatac agcaattaaa tgagactatt
    16501 accagtgttc ctgaaattag gaaaattggc gaaaatatta atcggcaact aaatagcata
    16561 attggttcag tgtattctcc taatcttctt ctttcctcaa ctatgtcaga tgatatgggt
    16621 tctttagcaa aatttctttc aatgaagcca gaacaaaata tcgacttaga cttactaggg
    16681 ttaggacatc taaatatgat ttatttagct ttgaaaatag tcgaatttga agcgtgtcgt
    16741 tcaagagaac tacttaatat tatgttgatt gaagaaccag aagctcatat tcatcatcat
    16801 attcaaaaaa ctctgttcga aggattaaat cttcaaaaaa actatactca gattctcatg
    16861 accacacatt ctgtacattt agctgaagtg tctgaaatat ctcggatgaa tgtattaaaa
    16921 tcgtttaatg gcaaatctat cgttatgaat ccagcaaata atctgaactc atttgctaaa
    16981 gaaaagttaa aaaagaacaa tctaaatcta ataaacgcag tagagagata tttagatgtt
    17041 aaaagaaatg gattgctttt ttctaaaggt gttatcttag ttgaaggaga tgctgaagaa
    17101 atacttattc ctcaaatagt aaaaaaatta tttggtataa acttagacga acttggaata
    17161 ggtttgatta atgttggtag tacttcgttt gaatatatcg ctagcttgtt tgacgatgat
    17221 agaatacaga gaaagtgtgc tattgtaact gatttagatg aacaggcaat cccatcagaa
    17281 catcgattgt ataaaagcac tgcacaagag aaaggtcaag caagaaaaga aaaattatcc
    17341 tcccttttta atgaaaacaa ttgggttaat tgtttttatg ctaatacaac atttgaaatt
    17401 gagtttttag atgtgaataa agaaaatatt ctaacttatg tcagtccaat tatagatgac
    17461 gcattcaaag ataaaaaaac aataactgct tataaagaag aaatacaaag tgaacaatat
    17521 ttagaaagaa atttagcgat gttaaaacta gccaatcatg ttggtaaggg gtggtttgca
    17581 attgagttag gtgaaaaaat tgacttttta atcaaaattc ctaagtatat tcttaatgct
    17641 attgcttttg catgtcagga aatagtagat ctctcaattt ataaaaaaat tctactccat
    17701 acaatcgact tgtacgagga aactacagaa ttgtcagctt tatcagctag tttaaaaaca
    17761 agtgatacag ctgatttgaa tatagcaata atttctttat tagagtactt tggtaaagat
    17821 gaggatgttg tgtatctaat caatcagata gaaaattaca caaaaatact ttatgctgag
    17881 gtttcaaaat gacaaatatc ttcgaaaaaa tcgtaaatga tttaaattta gaacaaaaag
    17941 atgttgtgga aagtgaagat aacctttatg taaatgcatg tcctgggagt gggaaaacaa
    18001 gggtacttac aaggaaaatt gcttaccaag caattaaaca cgatggatca ctaaaaagaa
    18061 taattgctat cacatacact aatagagctg ctgaagaaat taaggaacgc ctaagtttac
    18121 ttagtataga tgatgatatt aatatttggg tggggacaat acatcagttt tgtcttgaat
    18181 ttat
//
LOCUS       DAYYDS010000054        18124 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000054 DAYYDS010000000
VERSION     DAYYDS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.054"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..727)
                     /locus_tag="ACHA02_001837"
     CDS             complement(29..727)
                     /locus_tag="ACHA02_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8670159.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACHA02_001838"
     CDS             complement(720..2072)
                     /locus_tag="ACHA02_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8670160.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACHA02_001839"
     CDS             complement(2295..3695)
                     /locus_tag="ACHA02_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8670161.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACHA02_001840"
     CDS             3856..4593
                     /locus_tag="ACHA02_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8670162.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACHA02_001841"
     CDS             4718..5197
                     /locus_tag="ACHA02_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8670163.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACHA02_001842"
     CDS             5194..5961
                     /locus_tag="ACHA02_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8670164.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACHA02_001843"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACHA02_001843"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8670165.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACHA02_001844"
     CDS             complement(6864..7466)
                     /locus_tag="ACHA02_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8670166.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACHA02_001845"
     CDS             7572..7802
                     /locus_tag="ACHA02_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670167.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACHA02_001846"
     CDS             7793..8041
                     /locus_tag="ACHA02_001846"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8670168.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACHA02_001847"
     CDS             8147..8731
                     /locus_tag="ACHA02_001847"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8670169.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACHA02_001848"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACHA02_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8670170.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="ACHA02_001849"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="ACHA02_001849"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8670171.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="ACHA02_001850"
     CDS             10632..10811
                     /locus_tag="ACHA02_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670172.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="ACHA02_001851"
     CDS             10833..11858
                     /locus_tag="ACHA02_001851"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8670173.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /gene="glyA"
                     /locus_tag="ACHA02_001852"
     CDS             12033..13277
                     /gene="glyA"
                     /locus_tag="ACHA02_001852"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8670174.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="ACHA02_001853"
     CDS             13430..14377
                     /locus_tag="ACHA02_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8670175.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="ACHA02_001854"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="ACHA02_001854"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8670176.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="ACHA02_001855"
     CDS             complement(15279..15521)
                     /locus_tag="ACHA02_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670177.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="ACHA02_001856"
     tRNA            15642..15714
                     /locus_tag="ACHA02_001856"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="ACHA02_001857"
     CDS             complement(15833..16471)
                     /locus_tag="ACHA02_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8670178.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16594..17226)
                     /locus_tag="ACHA02_001858"
     CDS             complement(16594..17226)
                     /locus_tag="ACHA02_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8670179.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17577..17876
                     /locus_tag="ACHA02_001859"
     CDS             17577..17876
                     /locus_tag="ACHA02_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8670180.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6212 a         3162 c         3571 g         5179 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    16621 tctagcaaaa ccaagtgctt gcaactgatt aagcgtacaa ttttttagat gttccgagcg
    16681 tacttcaatc atttcaaagg agttgctact taaatcgata ttttttaaat cagtatcaaa
    16741 aaagctagca ttttctaaat cattagtaga aaacattatt ttagtgaggg aacagtctac
    16801 aaaatctgcg cttgataaga cagagtgctc aaaggtactt tcttttatgt ttgaaccaga
    16861 taaatttaca tagtccattt tacaattctc gaataacgtg ttcccaatat aactttccat
    16921 caatttagta cctgttaatt tacaattgac aaatttcacg cgatgcaaca tgctttttgt
    16981 caatatacag cctgaaaaat cacagttttc gaatatacag tcggtaatct ccattctttg
    17041 aaaatcattg gaagcaaaaa atgcatactt gaaaatagat ttgtaaataa ttggatggca
    17101 gattatttgg ttttcgatga cctctttttg tgaaaggata ttatacagtt caaaatcttc
    17161 gttttcatag atatgaaaaa aggatccttt attcaagctt gcttcattaa ctttaggaaa
    17221 aatcatattt aattacctcc attttcataa agtcatatta acaaaagcta aaccaactta
    17281 cattagcaga aaatgagata ggttttcgcc tttttcgata acgatagtgt cgctatctct
    17341 tgaaacgttc ttagaattgc cctgagagac acaaattagg cgtaattcta ataaaaatct
    17401 tagaataaaa atttttactg atttaattta gaatgttttt aaaataaaaa tcgttgccat
    17461 aacagtgtat tgaaaggttt taacaatcca gtaatgattt aaaaaaatct agatgtagcg
    17521 tatactttgt ttatagaatg tagtgggaag aaatatattt ttgggaggaa atcgtgatgt
    17581 ctaaaagtga agaaatagaa aacatggtac taaaattata tgatctcgtt ttaaatccag
    17641 agatcaaaga aaaggaacgt atacttttga tagacgcaaa gacaggctta gaaaaaggtc
    17701 agtattatcc taaagtcata aataatttgg aacgtagttt acgtccgtta gcaatcaggg
    17761 gagaattgtc taaaccagta tcaccgtttt atatggagat atcaacaata ggaaaatttg
    17821 aaaaagaatt aggtcgagga atggcttcgg caccgattac ttttgggcat ttataaaaac
    17881 gagttattca tctcaatgaa ttaaaaagta tagtcaatct cataatgcag gctaggttca
    17941 tttatttgaa cagagtctgt atttttttat tctaaataaa gaaacagatg tttctttata
    18001 ttgtatttta tcctttaatt aacacaaaaa cagctagtat aaactaataa tgttaattaa
    18061 aagtgtattt tatgaaacta tcgttattta ttaatgttgc atttttgtaa gttaaaaatt
    18121 ggaa
//
LOCUS       DAYYDS010000055        16685 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000055 DAYYDS010000000
VERSION     DAYYDS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.055"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..257)
                     /locus_tag="ACHA02_001860"
     CDS             complement(<1..257)
                     /locus_tag="ACHA02_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670181.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAF"
     gene            complement(273..923)
                     /locus_tag="ACHA02_001861"
     CDS             complement(273..923)
                     /locus_tag="ACHA02_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670182.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(934..2106)
                     /locus_tag="ACHA02_001862"
     CDS             complement(934..2106)
                     /locus_tag="ACHA02_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8670183.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2125..2997)
                     /locus_tag="ACHA02_001863"
     CDS             complement(2125..2997)
                     /locus_tag="ACHA02_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8670184.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3019..3939)
                     /locus_tag="ACHA02_001864"
     CDS             complement(3019..3939)
                     /locus_tag="ACHA02_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670185.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3939..4208)
                     /locus_tag="ACHA02_001865"
     CDS             complement(3939..4208)
                     /locus_tag="ACHA02_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670186.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4230..4586)
                     /locus_tag="ACHA02_001866"
     CDS             complement(4230..4586)
                     /locus_tag="ACHA02_001866"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFM8670187.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFLFCPNDWLS
                     CVTGSLLLTDKRVNRR"
     gene            complement(5053..6516)
                     /locus_tag="ACHA02_001867"
     CDS             complement(5053..6516)
                     /locus_tag="ACHA02_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8670188.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="ACHA02_001868"
     CDS             complement(6580..9597)
                     /locus_tag="ACHA02_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8670189.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="ACHA02_001869"
     CDS             complement(9748..10587)
                     /locus_tag="ACHA02_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFM8670190.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="ACHA02_001870"
     CDS             10732..10977
                     /locus_tag="ACHA02_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670191.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="ACHA02_001871"
     CDS             11040..11462
                     /locus_tag="ACHA02_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670192.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="ACHA02_001872"
     CDS             complement(11556..11873)
                     /locus_tag="ACHA02_001872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670193.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="ACHA02_001873"
     CDS             complement(11900..12106)
                     /locus_tag="ACHA02_001873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670194.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="ACHA02_001874"
     CDS             complement(12087..12269)
                     /locus_tag="ACHA02_001874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670195.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="ACHA02_001875"
     CDS             complement(12283..12633)
                     /locus_tag="ACHA02_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670196.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="ACHA02_001876"
     CDS             complement(12659..12961)
                     /locus_tag="ACHA02_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670197.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="ACHA02_001877"
     CDS             complement(12964..13197)
                     /locus_tag="ACHA02_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670198.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14101..14631)
                     /locus_tag="ACHA02_001878"
     CDS             complement(14101..14631)
                     /locus_tag="ACHA02_001878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670199.1"
                     /translation="MKMTPNHLRHICCEVTLFNRQRWKLSNRIVRRIIVDSTGKSTVI
                     LTKKLATKQDFQLFFSQVKTIFFDGKIIANRGCSNQDLMVSNIVFSRMKRRQDLLIQA
                     RKAYKVFKKQEKQSRGKQNEQIMNKKCIEVLNGKNDHSDTKKKEGRHENYPILLVCPN
                     CFSCGFVGRIGFRTWN"
     gene            complement(14628..15743)
                     /locus_tag="ACHA02_001879"
     CDS             complement(14628..15743)
                     /locus_tag="ACHA02_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8670200.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="ACHA02_001880"
     CDS             complement(15749..16147)
                     /locus_tag="ACHA02_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8670201.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4440 a         3471 c         2603 g         6171 t
ORIGIN      
        1 aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa agcatgacaa
       61 atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat ccgccacaaa
      121 acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc ataagaaaaa
      181 gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac ctctcttatt
      241 gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg ttgattaaag
      301 ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa ggttacgtca
      361 aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata cgacaccaca
      421 acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca tttctctaat
      481 tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata ttgcttcatc
      541 agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga atcaggctca
      601 taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg aatgtttcct
      661 ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt cgtgacctcg
      721 ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg tcctttcttc
      781 tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt tagctgtagc
      841 ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc gcctacacaa
      901 acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc gagcaaaaca
      961 aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt cacctacacc
     1021 gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc aatcttccgc
     1081 taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa tgataatatc
     1141 gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc ctcttgcgtc
     1201 gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc ctgctttcgt
     1261 taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg gattttcgac
     1321 actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca gactttttgc
     1381 tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta gacaataatc
     1441 ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag cgacgggatc
     1501 actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac gattccccat
     1561 cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt cctctacgct
     1621 ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat gaatcaaaaa
     1681 ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa aatttttcaa
     1741 agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg gatcgtttgg
     1801 tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta acatacccgc
     1861 tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa tttgaagccc
     1921 gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt cggttggact
     1981 acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa acagaaaacc
     2041 aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa gaagtttttt
     2101 cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc ttttgcgcca
     2161 aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa cgagagacac
     2221 ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca ccgcaatatt
     2281 tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc ctgattcttt
     2341 ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt caattggtgt
     2401 acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt gaacattttg
     2461 tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt cctgtactgg
     2521 aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag tcgtatttcc
     2581 ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac catttcctgg
     2641 gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat ctggattttc
     2701 tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg ttggtggaat
     2761 ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt ctcctccatc
     2821 tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg gtggaataat
     2881 ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg cactggttcc
     2941 taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt ttttcatgtg
     3001 tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg gaagcgtgga
     3061 aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg tttcttgtgt
     3121 ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc cttctttctt
     3181 catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt gtttcgcaac
     3241 tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca tttcacccga
     3301 ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt ttgtcgcaag
     3361 ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta gttgttcaat
     3421 ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga cgataaatcc
     3481 aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc tttgtgtaaa
     3541 cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg gtcgacgttc
     3601 gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta aggtttgaat
     3661 aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt caatcaatga
     3721 aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg gaatttcatg
     3781 gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca atagctcttc
     3841 attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat ctgttagtaa
     3901 agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac ttcttccacc
     3961 acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac ccgatattct
     4021 tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt atattcgttg
     4081 cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc aatctttgga
     4141 aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat tcctgttact
     4201 ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt gtcggtaagc
     4261 aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
    16561 gatttatcat aggataaaga gtgatagttt aatacttaaa gagacatatt aattttattt
    16621 aaaaattgac attaatgact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
    16681 ttggg
//
LOCUS       DAYYDS010000056        16392 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000056 DAYYDS010000000
VERSION     DAYYDS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.056"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="ACHA02_001881"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="ACHA02_001881"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8670202.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="ACHA02_001882"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="ACHA02_001882"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8670203.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="ACHA02_001883"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="ACHA02_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8670204.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="ACHA02_001884"
     CDS             2857..3732
                     /locus_tag="ACHA02_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8670205.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACHA02_001885"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACHA02_001885"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8670206.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="ACHA02_001886"
     CDS             4629..5063
                     /locus_tag="ACHA02_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8670207.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="ACHA02_001887"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="ACHA02_001887"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8670208.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="ACHA02_001888"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="ACHA02_001888"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8670209.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="ACHA02_001889"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="ACHA02_001889"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8670210.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="ACHA02_001890"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="ACHA02_001890"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8670211.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="ACHA02_001891"
     CDS             13334..14260
                     /locus_tag="ACHA02_001891"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8670212.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="ACHA02_001892"
     CDS             14442..15335
                     /locus_tag="ACHA02_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8670213.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="ACHA02_001893"
     CDS             15469..15594
                     /locus_tag="ACHA02_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8670214.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="ACHA02_001894"
     CDS             complement(15728..16360)
                     /locus_tag="ACHA02_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670215.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5825 a         2791 c         3494 g         4282 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aa
//
LOCUS       DAYYDS010000057        16372 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000057 DAYYDS010000000
VERSION     DAYYDS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.057"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACHA02_001895"
     CDS             complement(152..550)
                     /locus_tag="ACHA02_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8670216.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACHA02_001896"
     CDS             complement(547..1320)
                     /locus_tag="ACHA02_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8670217.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACHA02_001897"
     CDS             complement(1317..2000)
                     /locus_tag="ACHA02_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8670218.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACHA02_001898"
     CDS             complement(2167..3054)
                     /locus_tag="ACHA02_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8670219.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACHA02_001899"
     CDS             complement(3051..3920)
                     /locus_tag="ACHA02_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8670220.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACHA02_001900"
     CDS             complement(4057..5343)
                     /locus_tag="ACHA02_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8670221.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ACHA02_001901"
     CDS             5616..6194
                     /locus_tag="ACHA02_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8670222.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ACHA02_001902"
     CDS             6194..7123
                     /locus_tag="ACHA02_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670223.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ACHA02_001903"
     CDS             7120..8856
                     /locus_tag="ACHA02_001903"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8670224.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACHA02_001904"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACHA02_001904"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8670225.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ACHA02_001905"
     CDS             complement(9752..10261)
                     /locus_tag="ACHA02_001905"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8670226.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACHA02_001906"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACHA02_001906"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8670227.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ACHA02_001907"
     CDS             complement(11562..12653)
                     /locus_tag="ACHA02_001907"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8670228.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACHA02_001908"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACHA02_001908"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8670229.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACHA02_001909"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACHA02_001909"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8670230.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACHA02_001910"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACHA02_001910"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8670231.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4724 a         3620 c         2910 g         5118 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aa
//
LOCUS       DAYYDS010000058        16147 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000058 DAYYDS010000000
VERSION     DAYYDS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.058"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            225..893
                     /locus_tag="ACHA02_001911"
     CDS             225..893
                     /locus_tag="ACHA02_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8670232.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1093..1479)
                     /locus_tag="ACHA02_001912"
     CDS             complement(1093..1479)
                     /locus_tag="ACHA02_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670233.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACHA02_001913"
     CDS             complement(1451..2833)
                     /locus_tag="ACHA02_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8670234.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACHA02_001914"
     CDS             complement(2989..4176)
                     /locus_tag="ACHA02_001914"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8670235.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACHA02_001915"
     CDS             complement(4183..5214)
                     /locus_tag="ACHA02_001915"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8670236.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACHA02_001916"
     CDS             complement(5230..6684)
                     /locus_tag="ACHA02_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8670237.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6859..7764
                     /locus_tag="ACHA02_001917"
     CDS             6859..7764
                     /locus_tag="ACHA02_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8670238.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACHA02_001918"
     CDS             7855..8532
                     /locus_tag="ACHA02_001918"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8670239.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACHA02_001919"
     CDS             8529..8699
                     /locus_tag="ACHA02_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670240.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACHA02_001920"
     CDS             8813..9709
                     /locus_tag="ACHA02_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8670241.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACHA02_001921"
     CDS             complement(9764..11134)
                     /locus_tag="ACHA02_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8670242.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACHA02_001922"
     CDS             11372..12118
                     /locus_tag="ACHA02_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8670243.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACHA02_001923"
     CDS             12115..12954
                     /locus_tag="ACHA02_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8670244.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACHA02_001924"
     CDS             12955..13905
                     /locus_tag="ACHA02_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8670245.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACHA02_001925"
     CDS             14046..14798
                     /locus_tag="ACHA02_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670246.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACHA02_001926"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACHA02_001926"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8670247.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5239 a         3014 c         3241 g         4653 t
ORIGIN      
        1 gtccaatttc gtaaaacaac cgtaacggtt gtgtaaaaat atcattttag agcataaaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acagtgtcac
      121 agtccttttt ttagtaaaag gatacatttc ttccttgtat attccatgcg tgttcgtgag
      181 aaagcatata taatttaatt aacagatagt tagggggaga aaaaatgatt ttttcaatta
      241 caatgaaact ggtgttcggg ttgatcggtt tattattagt ggtacgtttg cttgggaaga
      301 aatccatgtc agaaatcaca ccgtttgatt tagtctatac gcttgtttta ggtgggatct
      361 tagaagaatc aacctatgat gacaatgtcc atgtagggca tgtcctattt gccattgcgc
      421 tttgggcagt aatgatttat ggtatcgaac gtatcgtcca aaaaaatgaa aaggtcaatc
      481 gttgggtcaa aggcgagcca tctgttctaa tcaaagacgg cgtcatcaac atgacagagt
      541 tgacaaataa ccatatcgag atggagcagt tgcgagcaat cttaagacag caagaatgct
      601 tttctcttga aaatgccaaa catgttatcc tagagaatgc tggtcaaatg agtgtattga
      661 aaaaatcaga tgaagacaaa gctttatcga tcctgcttgt agatgaaggg caaattcagc
      721 ataaagtgct tcagtccaat caattgactg aagcatggct tatggagaac ttgaagaaag
      781 aaggctatgc agatgtaaaa cagctgattt atgttgaatg gtcagaagaa aaagggctct
      841 acatactaaa taaagaagac gtcatgcatg aatcttaccg aatagatgga tagatagaca
      901 gaggttgtga aatcagcgcc ttggcttgtt actcaaaact aagcgacttt ttcacaacct
      961 ctgatatcta atatacgttg ttccagaagt tgcttcttcg aaaaataagc caaaattcca
     1021 caaaaattta agaagcaatt ttcgaaaaat cctttcttat tttttgaagt tgatcacttc
     1081 tgtcacaacc tctcatgtca caaaccattt ttttgtttcc tttaactcaa tataccgtac
     1141 gtcttcccac aatatcggct gatggtcaat cataaagtaa tcttcatagg cttcaccaat
     1201 gaagtgccct tcgacctcat ccaagagtct tccaaattca tcttttaaat tgagctgaat
     1261 gattactgct ttacgagata aaaaggcttc tgtcagtata ctatcgattt cttgcaatgt
     1321 catttgttgt ttaggaagag gctgtttgcg tgcttcttct tcattttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaaca actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgtcactg tttcaaatac tgatccagct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatacctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca attcctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctacattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agccggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa gtatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgcttttt tttatggatt attgcagtga atgttacaga atctgcgttt ttcattcaaa
    16141 aataaaa
//
LOCUS       DAYYDS010000059        15940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000059 DAYYDS010000000
VERSION     DAYYDS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.059"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(377..799)
                     /locus_tag="ACHA02_001927"
     CDS             complement(377..799)
                     /locus_tag="ACHA02_001927"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8670248.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(813..2219)
                     /gene="atpD"
                     /locus_tag="ACHA02_001928"
     CDS             complement(813..2219)
                     /gene="atpD"
                     /locus_tag="ACHA02_001928"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8670249.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2242..3144)
                     /locus_tag="ACHA02_001929"
     CDS             complement(2242..3144)
                     /locus_tag="ACHA02_001929"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8670250.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3158..4714)
                     /gene="atpA"
                     /locus_tag="ACHA02_001930"
     CDS             complement(3158..4714)
                     /gene="atpA"
                     /locus_tag="ACHA02_001930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8670251.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4737..5279)
                     /locus_tag="ACHA02_001931"
     CDS             complement(4737..5279)
                     /locus_tag="ACHA02_001931"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8670252.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5266..5790)
                     /gene="atpF"
                     /locus_tag="ACHA02_001932"
     CDS             complement(5266..5790)
                     /gene="atpF"
                     /locus_tag="ACHA02_001932"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8670253.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5881..6096)
                     /gene="atpE"
                     /locus_tag="ACHA02_001933"
     CDS             complement(5881..6096)
                     /gene="atpE"
                     /locus_tag="ACHA02_001933"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8670254.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6149..6868)
                     /gene="atpB"
                     /locus_tag="ACHA02_001934"
     CDS             complement(6149..6868)
                     /gene="atpB"
                     /locus_tag="ACHA02_001934"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8670255.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7170..7634)
                     /gene="smpB"
                     /locus_tag="ACHA02_001935"
     CDS             complement(7170..7634)
                     /gene="smpB"
                     /locus_tag="ACHA02_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8670256.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            complement(7656..10013)
                     /gene="rnr"
                     /locus_tag="ACHA02_001936"
     CDS             complement(7656..10013)
                     /gene="rnr"
                     /locus_tag="ACHA02_001936"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8670257.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            complement(10016..10771)
                     /locus_tag="ACHA02_001937"
     CDS             complement(10016..10771)
                     /locus_tag="ACHA02_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8670258.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10874..11110)
                     /gene="secG"
                     /locus_tag="ACHA02_001938"
     CDS             complement(10874..11110)
                     /gene="secG"
                     /locus_tag="ACHA02_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8670259.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11228..11506)
                     /locus_tag="ACHA02_001939"
     CDS             complement(11228..11506)
                     /locus_tag="ACHA02_001939"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8670260.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11619..12587)
                     /locus_tag="ACHA02_001940"
     CDS             complement(11619..12587)
                     /locus_tag="ACHA02_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8670261.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12802..12846
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12870..13844
                     /locus_tag="ACHA02_001941"
     CDS             12870..13844
                     /locus_tag="ACHA02_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM8670262.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13849..14424
                     /locus_tag="ACHA02_001942"
     CDS             13849..14424
                     /locus_tag="ACHA02_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8670263.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14491..15630)
                     /locus_tag="ACHA02_001943"
     CDS             complement(14491..15630)
                     /locus_tag="ACHA02_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8670264.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4354 a         3440 c         2853 g         5293 t
ORIGIN      
        1 atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt attgacaaag
       61 agcccaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat catctgaccc
      121 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg tttctctaat
      181 actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga agtagcttct
      241 tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
      301 atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa acacggataa
      361 acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt gctcggtgca
      421 acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct tcttcgattt
      481 ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca cgttcacgtt
      541 ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt ccgccattaa
      601 cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact tcatcaatcg
      661 tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca gctgtcgtac
      721 gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt accacattta
      781 ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt cgctttttct
      841 acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc atcgtatttg
      901 ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata tgaacctgga
      961 agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg gactctgcgg
     1021 gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa gatagcaatg
     1081 atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc tactttataa
     1141 tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa tggatctacc
     1201 gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc atccaaatgg
     1261 gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata gattgcttgg
     1321 atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg acccatttct
     1381 gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa ggctgatact
     1441 tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac atcttgacct
     1501 tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg catacgtgca
     1561 cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac tcctgaatct
     1621 ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc ggtaaataca
     1681 gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag tacggtttta
     1741 ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg ggcaagcaaa
     1801 tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc gtcaaaggct
     1861 ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt ttctaaatcg
     1921 atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac aggaacagag
     1981 attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt tgattccatc
     2041 gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac aacttttgat
     2101 ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa ggattggtct
     2161 aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc tgaactcatc
     2221 ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa tttcggtaat
     2281 ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct catcaatgat
     2341 attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat gttctgctgt
     2401 tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca ataactgaga
     2461 caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag ttgcttcttc
     2521 tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag ttagcgaatt
     2581 gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt acatagttgt
     2641 tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata gattgcgcag
     2701 ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc cgccaattgc
     2761 gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca tcgtttgttt
     2821 caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga cgatataacc
     2881 cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc ctctaggatt
     2941 ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt ctcttacttt
     3001 tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact tagaagcaga
     3061 caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga tacgtgtctt
     3121 gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga atggatttca
     3181 atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct ttatattcct
     3241 gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg attgtttcaa
     3301 agatatctga atgattggta tccagatatt cgaataattc atgttcgaag tctaaaatac
     3361 tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc actacttgtt
     3421 tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta cggcgtccac
     3481 gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta aatgcttcta
     3541 attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc attgctttga
     3601 tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct ggacgagtac
     3661 ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa ataacgtttg
     3721 ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag gcagtcattg
     3781 aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt gagtgtaagt
     3841 agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc aaggaaagtt
     3901 cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca tgtttcccgt
     3961 tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa agcaacggcg
     4021 ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca tattttttca
     4081 atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca tagatacaga
     4141 tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt ttccctgttt
     4201 tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga gcgtcgattg
     4261 cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg cctggtgcca
     4321 ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca attggctgac
     4381 ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc atgatctttc
     4441 ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg ataatgatac
     4501 ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa aattcaagca
     4561 actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca cctacataag
     4621 ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt tgttctttaa
     4681 tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc tttgtactat
     4741 ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc gattactcga
     4801 tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg ttttaattcg
     4861 actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg ttgttcgtct
     4921 gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc atcatatcga
     4981 cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc ataaacaact
     5041 tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat gatttctcgt
     5101 ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg tacttgttca
     5161 tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt tgaatcgatc
     5221 gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc tagtttcatt
     5281 tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt ctggagataa
     5341 ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat cgtcttttac
     5401 agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac tttttagacg
     5461 agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc cactttcttt
     5521 cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt gttcgcgttc
     5581 ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat cattggcgat
     5641 cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat gcttcaagag
     5701 agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca tcgcattgcc
     5761 aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt gaagtacttc
     5821 tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat actgattcaa
     5881 ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa cagcttcgac
     5941 tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag gttggcgagc
     6001 cattgattcg attgttttag agataacttg accgttacca taaccggctc cgattgctgc
     6061 tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc taaattagat
     6121 aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta tggctcatat
     6181 aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac aaggagaatg
     6241 cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct aagcttatta
     6301 ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca taaagacgta
     6361 aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac ataaaactat
     6421 atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc accccaaata
     6481 agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg tctgctgtcg
     6541 ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa ccgatgttgt
     6601 ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg ctcaattctt
     6661 ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat tctatcgcat
     6721 tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct aagaagaaaa
     6781 caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa attggtccaa
     6841 tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg aaattcacat
     6901 aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac acggtatcat
     6961 accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt ttttatttca
     7021 gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga aaaaacctat
     7081 gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta aaagcaaaag
     7141 caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa tgttcgatca
     7201 atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga ttttttccct
     7261 ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt taaaggaaca
     7321 acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt tttgtgtaat
     7381 aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc ataaggactg
     7441 atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc atcttttaga
     7501 ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat ccctgcttcc
     7561 atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat taattttccc
     7621 tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt cttcttttta
     7681 acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt actatcgcta
     7741 tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt tccttcgcgt
     7801 tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc ttctgcggaa
     7861 acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt tactcgcact
     7921 ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa atgattttca
     7981 acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc tactgtattc
     8041 ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc gaattcttcc
     8101 cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg ttctgtttct
     8161 actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc cgtagcccac
     8221 gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg atggacgatt
     8281 agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc cgctaagcca
     8341 taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag caacatagta
     8401 ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg taaatctttt
     8461 ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc aaaatcaaag
     8521 aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa cggcagattt
     8581 aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga ttcgatcaag
     8641 cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc agcatcaaca
     8701 aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg catcgtttca
     8761 aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc atagcgttgc
     8821 atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt catccgttct
     8881 gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt tggatcaatc
     8941 tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca gataccattc
     9001 gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact tgtccctctt
     9061 tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac atctgcaatg
     9121 tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc atcatctaga
     9181 tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc tctacgtcca
     9241 gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa tacatcatca
     9301 ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat gccagggtca
     9361 ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata ccctttttca
     9421 gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac tccctctgct
     9481 gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac gccgtaaaga
     9541 tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa ctgagtagtc
     9601 gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc tgtatttgct
     9661 cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt cgcttctttt
     9721 gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc tcgttcattt
     9781 gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt tcctttttta
     9841 ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag taatttaaaa
     9901 tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa actgcgtttg
     9961 cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt catatttatt
    10021 cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat tcgcgtcttg
    10081 ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc actcgctgac
    10141 ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca tctttcccag
    10201 cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg attgctgcct
    10261 gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact gctttttcat
    10321 tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat ttttcaaagt
    10381 tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg ccgccaatca
    10441 ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat cctaatacgg
    10501 caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg tctgcccacc
    10561 agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt cctttaaaaa
    10621 gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt cgaacatcgt
    10681 ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt tttgcataaa
    10741 ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac catagtttct
    10801 ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc cgttttgcga
    10861 gaggcttatt caactacttc gaagataaat atgctaggac aaataataag atcatccaga
    10921 tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga gctttttgtt
    10981 tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt tgcttgcttg
    11041 gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt cccaaaatta
    11101 aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt tatcatagat
    11161 aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa actttcagct
    11221 gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca aactcttttt
    11281 ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat tctactccag
    11341 tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca aatgtatggt
    11401 caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc tcttgttgtt
    11461 catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc cgcactcctc
    11521 aaataactga tattttttta ttcctacctt attttagcag tctcctgatc tataaacaat
    11581 tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt tattctttgt
    11641 tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga tgcttccaag
    11701 tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt ttgaatcgtg
    11761 tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa taaaggcaga
    11821 tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc ggcgtatttc
    11881 tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt gattttactt
    11941 aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat aaatcctttg
    12001 accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc cattgatccg
    12061 ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat ccaggcaatt
    12121 aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc tccgtgcgca
    12181 cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc tatatgagac
    12241 atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt attctgctta
    12301 tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt gattgttttg
    12361 ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg ttttttttct
    12421 actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata ccagtcatat
    12481 cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag aaaatggtaa
    12541 ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt acacccgccg
    12601 ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg attctttatt
    12661 ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag ttctcctcaa
    12721 tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac taatttcgtg
    12781 cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat aaaaaactaa
    12841 gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat tgcgatccag
    12901 ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc gaagtagttg
    12961 ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat gtgctgaaat
    13021 gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa aaacctttga
    13081 agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg gaaacaaatt
    13141 tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt ttggctgatt
    13201 atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc aatatcgctt
    13261 ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc tcgatgggtg
    13321 gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac tggtgtgatc
    13381 ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg gttgggttag
    13441 atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt caaatgaatc
    13501 ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc cactggaaac
    13561 aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat tttatcgagg
    13621 gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga ataagtcgtg
    13681 gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt aaaattatga
    13741 caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta aatgcacaag
    13801 aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat gaataaaaac
    13861 aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa ttatattgga
    13921 ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg aacgattttt
    13981 gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag cggtatttta
    14041 agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat tctcacaggc
    14101 ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag cttgaaactg
    14161 ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat cattaccgtt
    14221 cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct attacatggg
    14281 ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga ctaccttgat
    14341 cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca acgcttgata
    14401 tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata atgctaatct
    14461 ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa taattccttg
    14521 atatttttca atcatatcca aataattatc gatttcaaca tactcatcta cttgatgagg
    14581 caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag ctttcgtaaa
    14641 ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc tgaattgttg
    14701 ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt tgttgtaatc
    14761 gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga ttgattgcag
    14821 caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc cttggagctg
    14881 cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa cattatggat
    14941 tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt cagcatttgc
    15001 tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg gcatagagct
    15061 atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt gcgtatacat
    15121 caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta catagcctgc
    15181 tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg ctaataattt
    15241 gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa tcaccattgc
    15301 tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc catggatttc
    15361 tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga catccatatg
    15421 cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa tcaaattatc
    15481 tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca aaagcttatt
    15541 caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga tgatctcttg
    15601 taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat ttctttttat
    15661 tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat gaagaaagag
    15721 agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac gagaggactt
    15781 gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat gctagttatt
    15841 gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa acatcatata
    15901 cagaagctag ttgcaaaaaa aactctctac ctgctgatat
//
LOCUS       DAYYDS010000060        15072 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000060 DAYYDS010000000
VERSION     DAYYDS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.061"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..170
                     /locus_tag="ACHA02_001944"
     CDS             <1..170
                     /locus_tag="ACHA02_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8670265.1"
                     /translation="YQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRAR
                     IYLQQGLIFEN"
     gene            complement(553..1281)
                     /locus_tag="ACHA02_001945"
     CDS             complement(553..1281)
                     /locus_tag="ACHA02_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670266.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1259..2833)
                     /locus_tag="ACHA02_001946"
     CDS             complement(1259..2833)
                     /locus_tag="ACHA02_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8670267.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            3062..4000
                     /locus_tag="ACHA02_001947"
     CDS             3062..4000
                     /locus_tag="ACHA02_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8670268.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            4012..4923
                     /locus_tag="ACHA02_001948"
     CDS             4012..4923
                     /locus_tag="ACHA02_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8670269.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4947..6419
                     /locus_tag="ACHA02_001949"
     CDS             4947..6419
                     /locus_tag="ACHA02_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8670270.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6470..7081
                     /locus_tag="ACHA02_001950"
     CDS             6470..7081
                     /locus_tag="ACHA02_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8670271.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            7111..9024
                     /locus_tag="ACHA02_001951"
     CDS             7111..9024
                     /locus_tag="ACHA02_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM8670272.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            9152..12133
                     /locus_tag="ACHA02_001952"
     CDS             9152..12133
                     /locus_tag="ACHA02_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM8670273.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12130..13791
                     /locus_tag="ACHA02_001953"
     CDS             12130..13791
                     /locus_tag="ACHA02_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM8670274.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13831..14763
                     /locus_tag="ACHA02_001954"
     CDS             13831..14763
                     /locus_tag="ACHA02_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8670275.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4894 a         2389 c         3030 g         4759 t
ORIGIN      
        1 gctaccaatc gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg
       61 ggctgaataa caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata
      121 actttcgggc acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc
      181 ggcgcacaga acgcgccgca tatcggacta agttttattc aaatttgtct tcaccaacac
      241 gatttgacga agagcctatt taaataacta catccattta ttctatcttc taaaatagcc
      301 tttttcattt cagttaaata gttcagcgcc atttttgcac tcgacttgca tagtagtttt
      361 ttccatattc ctcaacctca gcgggggttg aagacataag gaaaatccta tgcgttatgc
      421 tcaactggat ctgattaaat attctaatgt atttttctct accatcatta aatggttaat
      481 agccaaaaat caccactttt caatggctcc ttgttgtata agtataacta gaagatgaac
      541 acgtctgaga aattatagaa gctttttacg gtaattactg gggctgtgtc cagtagtttt
      601 tttgaagaga acagaaaaat agctcacgtg atctattcct actttttgac taatttcatc
      661 aatagataag aatgtgtctt tcagtagagc acttgctctt ttgattcgaa agtctagcaa
      721 gtaatcatga aaggaataac ctgtttcttt tttgaaggcc cgtcctaaat aagcgggatg
      781 gtaatggaat tgtgtagcta catctttgag cgtaagcttt tcactataat tgttttgaac
      841 gtaatcaatc acttgttgaa ttgactcatt aaaagaatca tcagttagag agttttgatt
      901 agctgctgat ttacgcattt tttcttcttg tactaactta actaaataag gaaaatcaat
      961 gggtttcgtt atgtaacttt taatagataa atcaatcccc ttttttgcat actgaaaatt
     1021 ttggtgggca ctaatgacaa tactagtaaa gtagcgattg gatgtttgtt gcaaagattc
     1081 gattaattcg agtccagaaa ttttccccat cgtaatatct gtgataagta aatcaaagcg
     1141 ctggttttgc aggtttttaa atgcttcgtc tccgtctgtg tagcagtgat cgataatgat
     1201 gtcggggact ttcttcaaaa gataatgcct tatgccttct acaattgtac aatcatcgtc
     1261 aacaattgca attctcaaca tgcatattcc cccctttttg tattttgata gtaatcgttg
     1321 ttccgacgtg tagttggctt tctataacaa aacgtgtatc tccaggatag agatgcttta
     1381 tacgtcgaaa agaatttgag atgccgatat tctcactata aggaatatca tcattaagtt
     1441 tttgttgaat atgctccagc ttgtcttggt ccattccttt cccattttca acgattttta
     1501 acacataatg gttggttgtt tcagttgcat agacagtcaa acgattttcc ttttttgatg
     1561 aatcgaagcc gtgtttaata taattttcaa caaatggctg taaagaaagt tttggtaaca
     1621 ttaattgatc aatatgatca atcagctgat agtcaaagtt atgtcgataa cgacgttgat
     1681 gtagttgcaa ggagaggtcg atattaatta attcctcttt caaatcaatt atgtcgggct
     1741 gatatgtatt attgcgatac aacttgctca ataaatatat actgtcgctg atttcttcgt
     1801 catcattcaa tatggcagtc attcgaataa attccaaatt attaaataga aaatgaggct
     1861 cgatttgata ttgaagtgct gcatactttg cttcttgctt caaccaatta ttttcataaa
     1921 gtgttttttg atatgtagca ttatctaaat aaaactgatt gatatgcata gcgatttctt
     1981 tcaaatcacc gactttgttt tcgatattga ttgacttgta gtctcctgta gatgaatgat
     2041 ttaaggaagt aatgatttcc tgatactgct ggatatagtt acgaaaactt ctatttaata
     2101 attgatgaat agcaagaata aacaaactca ttaataagaa aatcatgatt gccagaccta
     2161 ataaggtttg tgacgaataa tttggatttt catatacttg taatgtgtgg ttaccgatag
     2221 ctatctgttg cgtttttttc catttagaag gcgagatgtc ttcagataaa ttgacggaat
     2281 caataccatt agacaaagaa taattgtaat gtgttggaaa gagactcttt tttagcgcgt
     2341 tgatcgaaac gtctagaatc aatgtagcat cgggcatgtc ctcactaatg aaacttaatg
     2401 aacgaataaa agaaattttt tcgttttttg taggtgcgta ggcacttaag tatttttcgt
     2461 aatctaatga gatagatata tttttaactt catcatcttg cacaagtttt tttaagttat
     2521 ctactaaata ggtaggggag cgatcctttg acaaaatatc ttccatgtat tcagcaggtg
     2581 atgagaggta gtagtcatta aggttttctt ggaaatcata attttgatag atctctgtca
     2641 cgataccgga gagaatacga ttatatgttt ccatatagtg ggtgatttgc tgaatgtttt
     2701 gcaggttttg atttttttct ttttcaatca cattgatata ggaaaagaac aatgtgaaac
     2761 aggcaagtaa agtaataaaa aaaatcaata tgtatgtaaa acgcataata acttttgata
     2821 aatcgttttt cattcaaagt cctcattttg ataatctcta attttatgaa tcattatagc
     2881 acatattaca agtgatgtaa acgctttgca aacagggcta cttatgattg tgaatacagt
     2941 ttccgtttaa aaactattct attgtagagg tttaataaac taaaaataat gtataaaaaa
     3001 ttaatgtaag cgattaacca agtgggtata atcaaggaaa cgctgagaga ggagagacaa
     3061 aatgaagcta aagaaagggt tattaggaaa tctaaaaaaa aaccgagcat tgtttattat
     3121 gcttttacca ggtgcgttat ggttatttat attttgctat ataccaatgt ttggagttgt
     3181 aatcgctttt aaagatttca gaatttcatc aaggggattc tttacaagtt tgttcaacag
     3241 tccttgggtt ggctgggaaa atttcaaata tctctttgct tcgcaagatg cttggatcat
     3301 tactcgcaat acggttgttt ataacgttgt aggtattttg ctaagtactt tttgtacagt
     3361 gtcattagca atcattctga gccaaatggc gaacaaacgg ttattgaaaa agatgcagac
     3421 atttatgatt tttcctaatt ttttatcatg ggtagtcatc agttactttg tttacgcatt
     3481 tttgagtacg gataaaggat taattaatat tttactaagc aattttggtg tggaacccat
     3541 aaattggtat agtgaaccga agtactggcc ttttattatt gtttttacga gtttatggaa
     3601 aggcgtaggc tatggatcca tcatttatta cgcgtcaatt atggggatcg atactagtta
     3661 ttatgaagct gctttagttg atggtgccag taaatggcaa cagataagga atgtagtctt
     3721 gccacattta agatcgatta ttgtcattat gttgattttg tctgttggta gtatttttcg
     3781 agctgatttt ggattgtttt atcaattgcc aagaaattcg ggggctcttt atgatgtgac
     3841 gaatgtatta gatacttatg tctaccgtgg attaatgaat agcggagata tcggaatgtc
     3901 tacggcagcg ggattatatc agtcatcagt tggctttgtc ttgattttga ttactaataa
     3961 gatagtcaac aagttagatc ctagttcagc actattttag gaggaatcaa aatgatatca
     4021 aaattaaaac ggcagatgta cttttcgctg ccagtcgaac ggtttttcac aatattacta
     4081 atcttattta ctcttagctg tatttttccc tttattttcg taatcgtcat ttcattaaca
     4141 gatgaaaaag cattacaact caacggttac gaattattgc cggctcaatg gagcactgat
     4201 gcatatcaat acttaataca agatggcgga cagttacttc ggtcattagg cgttacaatt
     4261 atgattacag tgattggaac gttgattact gtttttatga cgggaaccta tgcttatgtt
     4321 ttatcaagag cctctttccc ttaccggaag ttctttactt tttatctttt ttttaccatg
     4381 ttatttgcgg gagggatggt tccttcttac ttagtgatga cgaagatgct tggcttaaaa
     4441 aatacaattt gggctttgat tctacctctt gcttttagcc catacaatgt cattatttta
     4501 aggacattct ttaagaagtc gattccagaa tcgattatcg aatccgctaa aatggatggc
     4561 tgtagtgagt ttcgagtttt tttccaaatc gttttgcctt tagcgatccc aggggttgca
     4621 acaataggtt tattttcaag cttaggttat tggaatgact ggttcaacgc attgctgtat
     4681 attgacacca acaaattgat tccattgcaa tatttgttaa tgaagattca aaattcaatg
     4741 gaattcttag caaataataa tgacattaca ttagcgcagc aacaagcaat tcaaaatagc
     4801 cttcctcaag aatcaacacg aatggcaatg gtcgtagtag ctactttacc gattgcaatt
     4861 gtttatccgt tcttccaaaa atactttgtt caaggattga cgatcggtgg agtgaaagaa
     4921 taaaaaagaa taggagatgt taaaagatga aaaagaacat gcagaaatat ttgattggtg
     4981 gtgcttctgt actaaccctt ggcctattag gtgcttgtgg tagcagtaat ggcgaagcaa
     5041 ataatgaaac aggaaccaca aaagttactt ggtatcaggt tggagaagaa cccaaagatt
     5101 ttgatgaggt catgacaaaa gtcaacgaaa aattaaagga agaaatcaat gtagagttgg
     5161 atatgcggct gattccggac ggggattatc agcaaaagct tggtgtgatg atcaattcag
     5221 gagaagaata tgatatttgt ttcgtcaatg gcacagatta tgtgaattat gggaacaaag
     5281 gtgctttcat tagtctcaac gatcatttgg ataaagagtt gaaagattat gcagctgaat
     5341 tgaatgaagg atttattgaa ggtggggcaa ttaatggcga aacttatgga ttgccagtca
     5401 ataacgatat tgccacaacg gaatattgga cctttaataa agatttggta gatcaatacc
     5461 aattggatgt agagaatgtt gaaggtgtag aaacattggc tccagtatta gaaaagtttg
     5521 cagaaaaaaa tactgatacg aatattacgc caatcggtat gacccgtacc agtgtctgta
     5581 ttccttatga caatattatc ggtggtggtg taccgctagc ggttgcattt gaaggcgata
     5641 cacaaacagt tgttaatact tatgagactc cagaaatgat gacttatttg aaggctatgc
     5701 atgagtttta taaaaaagga tatattttga aagatgcagc cactgttacg acagatccat
     5761 ttcgaattga tagtaaaaac tggtttttag gtcgtaactc aagtggttat caaccattag
     5821 cacaaaaaaa attagaagct ctagcgggac atggattaat tttaaaacca gctggaaaag
     5881 cttttaaaac gactggattt gcaagagtgg caatgcatgc ggtatctgct tcaagtaaac
     5941 atccggaagc agccttgaaa ttgttgaatt atgtgaatac caatcaagaa gtagcggatt
     6001 taatggttta tggaattgaa ggaaaacact atgatcgcaa tgaagatggt tcgattactt
     6061 taacaaactc tgatgattat tatcaaggcg cttgggcatt ggtaaatact tatctagttt
     6121 caaaaacttc accgattaca gaagaaagta aaaaactaga agaagaaagt aaaaagtttc
     6181 aaaatgaagc accgatgtct cctattttag gtttctcttt tgacgcttct gattttcgaa
     6241 cagaaattgc aagtgtacaa aatatcgtgt cacaatatga agtactttta agctcgggaa
     6301 cagttgaccc tgaacaatat gtaccagagt tgttagaaaa actagaaaaa gctggattgg
     6361 ataaaattca ggcatctgct caagaacaat acgatgaatg gcgaaaatct aacaaataat
     6421 tttttactaa atgaaaagaa tagactaagc agttagtggg gggattcaaa tgaacgaaaa
     6481 caaatggacc aaaggattta attgggtttt tcaattgttg ttactaaact ttacttattt
     6541 tatactaatt tgtttaggat ttggaatttt aggttttttc cctgctacga tagctttgat
     6601 tgctggcgtt gtgaatcgaa aaaaatatgg cttgcaagag tctgtgaagt tcatgagtaa
     6661 aatctataag aaaaattttg ttagatataa tctatattgc tggtcagcaa ctttgttgtt
     6721 ggccggcaat attactacga tctggctttg ttggcagcaa agcgcaccat tgttttattt
     6781 attaagtaca ttattatcgg taattacact gttcctagtc tttgcaatgt tgaattgggt
     6841 cgtgatcaaa agtcttgggt tgtggatgat gggagaccga ctgattgact atttgctttt
     6901 cacagcaacg atgttgccaa cggtagtcat gcaaggcttg gtgcttggaa tttatttccg
     6961 aatattgatg attttgcctg tgatttttct gtttggcgga acagctgtaa cggtaatatt
     7021 gattagtaaa ttaactgaaa gagcgttagc aaagttgcct tgtcagatac aatcgcacta
     7081 attttttcac aaaaaggagt atttctttct atgaagttaa ctgataaaca agtagctaat
     7141 atagccttta tccagcaact tgagattcca ttgacacaaa aagatgaaga acaaatagac
     7201 ttgattttta ctgacacaaa ggctaatgtg cgacaaatca agattactaa aaatgagcag
     7261 caacgaattt gcttgtctta ttcacatgaa gacttgctgt atcgaggatt gtttatctgc
     7321 cttccacgtt ttttagctca aggaggagag atgccataca ttgaagaaac ctcgatcatt
     7381 gagaccagtt attcattaga tgcttctcgt aatggcgtga tgaataaaga gactctaaaa
     7441 aaattcattt cttacttagc gttgatgggg tacaacacat tgtatattta cacagaagat
     7501 acctatgaag tagaagacct tccatatttt ggttaccttc gcggagcgta tacaaaagaa
     7561 gaagtgaatg aaattgttga atttgcttct ggttttggca tagaagtagt tccatcgatc
     7621 cagacccttg ctcacttgac ccaatttttg aagtggtatg ctcaacaaga actgatggat
     7681 gatcaaaata ctctactgat cggtgaagaa aaggtttatg cggcaatcga aaagatgatt
     7741 attgcctgca aagaaatgta tcagacagag cgtattcatt tgggaatgga tgaagcctat
     7801 caagcaggtc tgggacgata tttaagcctg tatgggtatt gtgatcggac aactttaatg
     7861 attcaacata tggaccgtgt gttatcaatt gttgataaat ataagatgaa accaatgatt
     7921 tggagtgatt tcatttataa gctattagat gaaaccggta aagataggta ttacaatcct
     7981 gattttaaac tcaaagcatt agcagctaaa ggattaccag caggattaac ctatgttcat
     8041 tgggactatg gttcagatga agtttcctcc tatgagaaag taattgataa ccatttgaaa
     8101 ttttgtgaca ttgatcatta cgttttagct agtggtgccc atatctgggg gagactagcc
     8161 ccgaatcatg ggaaaacaga agcaattgtt tctgcagctg taaaagcttc taaaaataaa
     8221 ggtgtacgtt ctgtgatgtt aactacttgg ggagatgaag ggcaggaaac agagcattgg
     8281 cattccttaa ttggggcatt aaaattagca gattatgtct attctgaaga agagtggaat
     8341 gattttgagg aaatctacga tcgtttatta ggaacaggta gttatcaatt aatgtccgaa
     8401 ttacgatatt tcgatgaagt ggataccatt acaaataaca acttacaaat ggcttccgtg
     8461 agtaaactgc tactttggca agatccgtta gttggtatat atgactttca tgtagccgct
     8521 cataacaaaa ttgccactag aacaattggt acttattatc atgagttagc tcagaaatta
     8581 gatatgaata aaaattggaa tccaaaacaa ccaattttaa caactattcg tcaacgatat
     8641 atacttttag ctgaaatttt agcacaaaag gcagaactag gcatctattt acaacactgc
     8701 tgtctggtag aagaccttgc aggattaatg gaattagcga atcggtgtga agcactagcc
     8761 aatcgagtaa atgaattggc atctattcat gaaataattt ggtatcaaac atacaaaacc
     8821 tatggttggg aagtattaga acaacgatat gctgcattag ctcatcgtct gaaaaccgtt
     8881 gcgcgtcgta ttaaaaatca tcttcagggg caagatgccc tagaggagct tttggaagaa
     8941 cgattaccgt tttgtcaata tcaagtgccg cttgaaattt ctggctttaa ttatcggcaa
     9001 agctcggtgt caggctacaa ttaacgaagg aatcttcccg ttgaacggac cacttgctca
     9061 tctaatggga aagtggttcg ttttctattc ataagtagct taccactaac tcttttatta
     9121 agaagagaaa aaaagtagga agtgagaaaa tatgttatcg gactattatc aaaatctgtc
     9181 agttataagt gtcaatacag aaccattacg taattattat attccttatg caagtaaaga
     9241 agaagcaaaa caagagttag aaagggaaaa aacgagccag tttatcgatc tcaatggtac
     9301 atgggatttt cagtattatc cttctgtcca acagttaccg gaaaattttt ttcaagaggc
     9361 ctttactgaa agctatgata gaatttctgt tcctggagtt tggcaaacag agggatttga
     9421 cttacctcag tatatcaatg tatcttatcc aattcctttt gatccgcctt atgtacctaa
     9481 tgatacccct tgtggtgtat atcggcgtca gtttcaaatt gagcaaacag atgcacgctt
     9541 ttacttaaat ctagaaggag cagatagtaa ttgttttgtt tggataaata gaaactttgt
     9601 aggctatgac caagtgagtc atagtacatc tgaatttgat gtgaccaagt atttacaggt
     9661 tggggttaat gaaattcaaa ttctagtgat gaagtggtgt gatggtacct actttgaaga
     9721 tcaggataag tggcgaatga gcggtctatt tcgtccagtg tatcttcttc aacgaccaac
     9781 tatgcatctg acaagttatc aactacggac gttgactgat cctaaaaaag gaagcggaac
     9841 attaatggtt tcttttcaac gatttactcc tattgaagta tcgttaccta tttcttacga
     9901 attattccat cctgatggac gtctcatagc gaaaggggag tgttactcgg aacaacaaat
     9961 aaatttggat gttgaagatt gccttttttg gacagcagaa acgcctaaat tatatacatt
    10021 acttttaaca tgtaatggtg aatattttaa agaaaaagtt ggctttcgag cactgacgat
    10081 caagaatcgt aagttattgc tgaacggaac acctattaaa ttatatggag tcaactatca
    10141 tgacgtgaat cagcaaggca cgccagtgat gacccgagag cagttacgtg cagatttgtt
    10201 attaatgaaa aaattaaatg tgaatactgt acgaacggca cattacccta aatcaccaga
    10261 tttttatgaa ttggcggatg agattggtct gtatgtaatc aatgaagcag atttggagtg
    10321 tcatggtgtg gttaatctat atgatactga agcgaattat caactaattg ctaatgatcc
    10381 gacattcgaa aagccgatta tggaccgagt gatccgttct attcttcctt atcaaaatcg
    10441 aagctctatc attatgtggt ctatggggaa tgaatctggt tatggagtaa attttgaaaa
    10501 agctcaagct ttagcacgga aattggatga ttcacggttg attcataatg aacgctcgat
    10561 tgaaccagtt attgaccaag tgaatgattt tagtaacttg gatgttatta gtcaaatgta
    10621 tcccccactg gaagatgttc atcgtcattg tcaaagaagt gatactgaaa aacctttttt
    10681 tatgtgtgaa tatagccacg ctatgggcaa cggtcctgga gatcttaaaa cgtacttcga
    10741 tttgattcaa cagtatgatt cctttcttgg cggttgtgtc tgggagtgga ctgatcatgc
    10801 aatcaataga ggaactttag aaacgccatc ttatttatac ggtggtgatt ttggtgaaaa
    10861 atatcacgac ggcaattttt gcgtagatgg tttattgacc ccagatcggc aattaacagt
    10921 aggtgcattg gagtaccgta atattcattc gcctattcaa ctgattgctg tagatgagga
    10981 gctgcaagaa gtaaccttga gcaatataac tgcttttaca gccggttcag catttaacct
    11041 ttctcttgaa tggtatatag atggaaaaaa aacagtttat gaagtaatga atcctttcat
    11101 acttgagcct ggcaaaagtt gttcattgcc catcacagta cctacaacga ttcatggtgc
    11161 tcatacggtt gctgtttcgc tatctgttta taaaaatgat gagctttatg gacagcaaca
    11221 gctctttctt aaaaaagaaa aaatgagatt gccaagaact gctcatcata atgatgcacc
    11281 acaaatttca gtaaaaggca cagagctgat aatcaaagga gaatccttta tttatatata
    11341 tcaattagca aaagctgcgt tcacttctgt aaagatcaat caaaaggaac ttttggaacg
    11401 tcctactcaa ttttcaattt ggcgtgcacc aacggataat gatcgtaact tagtaaacaa
    11461 atggaaagca gctggatatg ctgaaatgtc tttagaaatc agaaaaaaag actggaaatt
    11521 cgtagatgat catctcctga tcgaaacgtc ttatcgagga gttattccag gattacagcc
    11581 aatttttaca atgcacatac agttgaaaat aaatggtgca ggcgtaatca accaaaaagt
    11641 atcagtggag aaaaatccga tttttccaga tttccctcgc tttgggatgg actggccgtt
    11701 gaatgatact ttttcgaagg tttcttattg tggctatggg ccgtatgaaa gttatcctga
    11761 taagcactat gctgcgatgt ttggtattta tgagatggag attgaggaat ttgatcagcc
    11821 atatatcaaa ccacaagaat atggtaaccg ctggcaatgt gattgggtat caatcaaaaa
    11881 tcatcaagga tctaccatga cggtttatgg aaactctaat ttttctgctc tgccttatag
    11941 tcaagctcaa ttaaccgaaa agtcacacca ttatgaatta ataaaagaaa atagaacttg
    12001 gttcaattta agttataaac aaaatggtat cggctcacac agttgcggtc cttatttaga
    12061 agatcgtttc cgttttatgg aagatacttt tgactggaat atagtgatta ctttcaaagg
    12121 agaaaataga tgaaagaaac aaagatcaat caagtcaaaa aaaaattatc tcgttatcaa
    12181 gaagttgttt atcgaaacca ttgcttaaaa tggtcaaaaa ataagctgaa tgttcgtttt
    12241 aatggattat tttatcgttt cgaacagtta gaagaagtct ggaaaccggt aacgaatttc
    12301 caatcatgtc ctgtacagct cataaatgat ccaaaagaaa catacgattt atgtataaca
    12361 cacctttata accagcattt gaaaaatgac ggattttatt taaaggttac taatgacaat
    12421 caacttacga ttgaatcagc aaaccttcga gggtttcaat atgctatgga agccttatta
    12481 gagagttttt tttcggtagg agatcaatta ttattaccgg aattgattct gaaacatgaa
    12541 ccatcagtga aaattcgagg aatcattgaa gggttttatg gtatcccctg gacacatgaa
    12601 atgcgcctct ctcttttttc atatatgaaa gataaccaat tgaatacttt tatgtatgcc
    12661 cctaaagatg atgaattatt acgtaaaaaa tggcgtgaac tctacgatgc gcaagaatta
    12721 gacaaattta aggagctttt atctgctgct gaggctagca atatcgattt ttggtactta
    12781 attagtcctg gaaatgatat tgatattacg tgtgaagaag acattcaagt tcttctgaaa
    12841 aagttagaac aactgattga gcttggcgta tttcaatttg gtctattaat ggatgatatt
    12901 gactatcaat taaaaggtgc tgctaaacgc cgttttcgag agccagcatt cgctcatgcc
    12961 tatttagtga atcgagtaga ggaatatctt agcactcggt tgtccaatca tgaattagtc
    13021 atttgtccta cagaatatga taatcgtcat ggttctcgct atttagcaac gttaagtcaa
    13081 gaaataccag aacaattacc gttcttttgg actggcccaa gcacgttagc ggcgtcaatt
    13141 tctacagaag agctgcagga aatggcttct gtctatcaac ggccgatgct gatttgggat
    13201 aatattccgg taaatgatta tttagaggat aaagaactat tgttcatgag tccatatgag
    13261 aatcgaacac cgaatctttc taaggagaga taccaggtta caggagtagt ttcgaatcca
    13321 atggctcaat tagaagcttc aaagtttacg atcaatagta tggctaatta cttatggaac
    13381 tgtgagcgtt ttgatccttt ggaaacttgg acgtcagtcg ttgaaaaagt agttggaagt
    13441 aaactacaac cagcttatct taccttaacc aatgcttttc caaatcatta cacaagcagt
    13501 ctgctttcag atgaagaatt actgctagcc aaagacccag aatgggtcac aaaagaaatg
    13561 gctgctttag tccaagcagt aaaatggatc cagcaagaac aacatacatc tagattgaga
    13621 acagaattgg agccttggtt acaagcaatc acagaaagtt ggactttctg gcaagaatgg
    13681 caattaaaaa aagatccaaa agaagcagaa gaatggttgg ctaagaaaaa aacacaccgc
    13741 ataggacgtg atttagtaac tgcccattta gagcaaatta tcaaaagtta gatatcttaa
    13801 aaaataaaaa gcaagaaaaa ggagaacaaa atggtcaaga gattaattag tgcaaactac
    13861 agtgatgtca agaaaatgac gtcaaaagag ttgaagcaat ccatcaaagc gagtgaagga
    13921 cgggtgattg tttgtgaaac ggttgctaat actcttccac aatctggaga aattacgaat
    13981 gcagaagttg cgtcagcatt tggtgcagat ttgattcttt taaatggttt cgattgttat
    14041 cgtcctgcag tttttggact accaaatgtg acgattgaga aggtttttga tctacagcaa
    14101 acgattgaaa acccgattcc taaattaaaa gaactagtcg gtcgtcctgt gggaattaat
    14161 ttagaaccca ttgcccctaa aacaaatatg atgagtgaga aagtagaaat cagccaagga
    14221 agagcaagct ctgttgaaac gatacaaaaa gcagaagaac taggagtcga ttttatttgc
    14281 tttaccggta atccaggcac tggagtaacc aatttagaaa ttactaaatc cgtaaaaatg
    14341 gcaaaaaagt atttttcggg attgattatt gctgggaaaa tgcatagcgc cggctcagaa
    14401 gagccagttg tgaataaaga ggcgattatt tcttatgcgg aagcaggcgc agatatttta
    14461 ctagttcctt cagtagggtc tatcccaggc tttttagagc aagacctaat tgaagcggtt
    14521 cggttggcaa aaaaatatga tttgttaacg atgtctgcaa tcggtactag tcaggaaagt
    14581 gcccgaccag aaacattgcg gcaattagcc attatgaata aaagttgtgg tgttgattta
    14641 caacatattg gagatgctgg atacggggga ttggcaccag cagaaaatat tttcgaaacc
    14701 tcggttgcaa ttagaggaat gcgacatacc atcaactgcg ttagccgctc gatcaatcgc
    14761 taacaaaatt aaataaaaaa tggttaaatt gattatttaa gtgggaacat acttgagata
    14821 tcagctaact tctgtcactt taaaaaaggg actgtgacag aagtgaaaat agacaaaaaa
    14881 tatccgaaca agagactagg atagcagaaa aactatcgtt ttgcaacgag gaccaactgg
    14941 accgtaggag caattctctt attgttcgga tatttcgctt gttcaaggac ttatgtctgt
    15001 agtgcccttt tttggtagga aaaatggtag ggaaccatat catttttaat gaaaatcaag
    15061 catttcaaaa gt
//
LOCUS       DAYYDS010000061        14492 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000061 DAYYDS010000000
VERSION     DAYYDS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.062"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..1272)
                     /gene="rpsA"
                     /locus_tag="ACHA02_001955"
     CDS             complement(43..1272)
                     /gene="rpsA"
                     /locus_tag="ACHA02_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8670276.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(1407..2087)
                     /gene="cmk"
                     /locus_tag="ACHA02_001956"
     CDS             complement(1407..2087)
                     /gene="cmk"
                     /locus_tag="ACHA02_001956"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8670277.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(2136..2771)
                     /locus_tag="ACHA02_001957"
     CDS             complement(2136..2771)
                     /locus_tag="ACHA02_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8670278.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(2837..4240)
                     /locus_tag="ACHA02_001958"
     CDS             complement(2837..4240)
                     /locus_tag="ACHA02_001958"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8670279.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(4227..5258)
                     /locus_tag="ACHA02_001959"
     CDS             complement(4227..5258)
                     /locus_tag="ACHA02_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670280.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5289..5510
                     /locus_tag="ACHA02_001960"
     CDS             5289..5510
                     /locus_tag="ACHA02_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8670281.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(5675..6412)
                     /locus_tag="ACHA02_001961"
     CDS             complement(5675..6412)
                     /locus_tag="ACHA02_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8670282.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(6565..7905)
                     /locus_tag="ACHA02_001962"
     CDS             complement(6565..7905)
                     /locus_tag="ACHA02_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8670283.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(8142..9665)
                     /locus_tag="ACHA02_001963"
     CDS             complement(8142..9665)
                     /locus_tag="ACHA02_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8670284.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(10043..10654)
                     /locus_tag="ACHA02_001964"
     CDS             complement(10043..10654)
                     /locus_tag="ACHA02_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8670285.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(10777..10902)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(10984..11700)
                     /locus_tag="ACHA02_001965"
     CDS             complement(10984..11700)
                     /locus_tag="ACHA02_001965"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8670286.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(11706..12275)
                     /gene="scpB"
                     /locus_tag="ACHA02_001966"
     CDS             complement(11706..12275)
                     /gene="scpB"
                     /locus_tag="ACHA02_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8670287.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(12259..13029)
                     /locus_tag="ACHA02_001967"
     CDS             complement(12259..13029)
                     /locus_tag="ACHA02_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8670288.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(13026..13397)
                     /locus_tag="ACHA02_001968"
     CDS             complement(13026..13397)
                     /locus_tag="ACHA02_001968"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8670289.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(13416..14303)
                     /gene="xerD"
                     /locus_tag="ACHA02_001969"
     CDS             complement(13416..14303)
                     /gene="xerD"
                     /locus_tag="ACHA02_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8670290.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4056 a         3131 c         2459 g         4846 t
ORIGIN      
        1 caaaagcagc tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc
       61 ggttgctttt tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa
      121 tccagtattt tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc
      181 tggtttttct tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc
      241 taatactttc actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg
      301 tttatgtgaa atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa
      361 ggcaccgaag cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt
      421 tgtatcgatt tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc
      481 ttgttcagga ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc
      541 acttggtttt gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc
      601 gcctaggtcg acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc
      661 tccctcatgg atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac
      721 cgctttatgc gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct
      781 caatgttttg cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc
      841 agggacaaaa ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac
      901 aggtgcttcg atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt
      961 agcatctaaa cggcgttttg aaagtaggta gctgccgttt tctttatctt tgccgatcgt
     1021 actgataacg actaaatcta atacatcacc gacttttact acttcgctga tatcttctac
     1081 cggcaaagtt gataattctt ttgccggaac gactccttca actccagccc cttcgattcc
     1141 gacaatcact tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt
     1201 tacttcttgt acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc
     1261 aaattctgtc ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt
     1321 ttagtttaat tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac
     1381 aattgttttt catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca
     1441 gcttttattg ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca
     1501 tcctctgctt gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt
     1561 atctctttcg tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa
     1621 cgtctttctg cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt
     1681 aatacagctg tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt
     1741 cgttggacag caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct
     1801 tttgtgacat ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc
     1861 tgtccatctt cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct
     1921 tcttcatctg aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg
     1981 gcaccggtat ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt
     2041 ttcccagatg aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt
     2101 tttctcagaa aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca
     2161 gggtaaagca taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg
     2221 atccctgcac gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa
     2281 ccggctgcag ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct
     2341 tgataagttg cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac
     2401 gttacacctg tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt
     2461 gaagtctcag ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa
     2521 gtttcagaag aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa
     2581 gcacctgctg gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat
     2641 gtatttcctt tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg
     2701 tagattggtt gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct
     2761 tttctgctca ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt
     2821 gtcttgtcct aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttt
     2881 cccaatgaag ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg
     2941 ctccatcaat atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta
     3001 agatgaattt tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga
     3061 attctttttc tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact
     3121 gctggatcag ctccatccat tttttttgca gctcatcaaa gggagcttgc tttgtggcat
     3181 attccaaata aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat
     3241 ggatacgttc atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc
     3301 ctgctcgacc gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca
     3361 cataacggat atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact
     3421 gcagctgatt ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac
     3481 cgtgatagta tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt
     3541 tttttgttgc acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt
     3601 cttgttcttt ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt
     3661 gtctgatctc ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat
     3721 ctgttgctgt cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc
     3781 ctaactgttg atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat
     3841 ctactacata taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca
     3901 acatttcggg actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc
     3961 tttcagattg cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta
     4021 attgcgtcac ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt
     4081 ccattaaata ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag
     4141 cgagagtatc ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg
     4201 aatcaaatcc aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc
     4261 aatagataga tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat
     4321 tcgctaaagc gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct
     4381 gcagagagaa tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga
     4441 tctgttactg ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca
     4501 ttcataaaca tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa
     4561 tcgatcaaag cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg
     4621 atacatctgc tttgttgcag ataaacctct gactcatcat tgttttttgc cagctgataa
     4681 ggaagcagcc ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct
     4741 tctggtatct ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca
     4801 cgtggcaatt ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc
     4861 tctgctggaa gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat
     4921 ttaatgagcc gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta
     4981 tgttctattt gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt
     5041 tcatttattt cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc
     5101 tgcttcaatt ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata
     5161 agtactgaag tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg
     5221 tgtcctgatg aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat
     5281 ggttagaaat gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt
     5341 gtcaaatcga agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc
     5401 aagaaccaga ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag
     5461 cgtatcaaaa atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga
     5521 aactttaaaa acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct
     5581 tgttccgtga aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact
     5641 agttttttca cggaccctat aggacccatt tatattattt caactttttc agccagtttt
     5701 ccaatgtatt gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc
     5761 ctttttgctc accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact
     5821 ctgttctttc cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat
     5881 cttttgtcgc cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt
     5941 cgggataact tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga
     6001 cataacgact tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg
     6061 gaaaatcttc tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca
     6121 ttttgacaat agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac
     6181 tttctggctc tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct
     6241 ttttctctcc ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt
     6301 gcgcttctga cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga
     6361 gtaatcctaa aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc
     6421 accttttcta tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat
     6481 aagagagaaa tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata
     6541 tccgcccttt ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata
     6601 aatttccatt gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa
     6661 gtcaggagta tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa
     6721 agtagcgccg cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc
     6781 caatagattt ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa
     6841 ttcttcataa gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc
     6901 tttcttgaat gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat
     6961 ttccaaactt gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac
     7021 gacagcttca ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa
     7081 atcaaatact tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc
     7141 atctttcgcg tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc
     7201 gaattcgtgt tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc
     7261 tgcatcagga acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg
     7321 tcctgtcaag taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc
     7381 tggcgcaaat ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca
     7441 taaattttct tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc
     7501 atcaatcaaa gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga
     7561 tccgcgacca actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt
     7621 attttcgtcc cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc
     7681 agagccaatc tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag
     7741 cttttcactg atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg
     7801 tccttgtcct gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga
     7861 ttctaatgct tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta
     7921 atatgttaaa aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga
     7981 cgttttaagc ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac
     8041 actcactgaa tggagctatt gacttgtcca cgtgcttatc gatagccgtt tatcggtttc
     8101 ttcattctta tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca
     8161 cctaggatca cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac
     8221 cacactgaaa catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt
     8281 gtaattaagt ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg
     8341 acgccggcaa attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa
     8401 gttgaaggaa tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca
     8461 cttgaagata atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat
     8521 ccattgttca taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc
     8581 aaatcagaag caccagctaa aaattcagag acaaattcag actctttcat gccataagtg
     8641 attgctacta cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca
     8701 cctaatggaa gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag
     8761 tttgtcaatg attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg
     8821 atgatcatga taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg
     8881 attacttctt gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg
     8941 ttgtatacta atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat
     9001 acaatagaaa tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc
     9061 atcgaaatcc caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct
     9121 agacagccta cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct
     9181 acagccatca tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg
     9241 taagtagagc cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt
     9301 cctttgaatt tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata
     9361 atacctaaaa atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt
     9421 tctttagtta gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata
     9481 tcccacagcc cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct
     9541 gcatcgtcta cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca
     9601 ataataataa ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt
     9661 tccatcttct tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt
     9721 tatcaaaaag agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa
     9781 aagttcgttt ttccctattc cctcctcttt tttcagtttt ttaaagcttt cttctttttc
     9841 attgtagcgt ttcctttttt ttataaacaa acattttcgt cttttaaaag gagaagaatt
     9901 tcacatttgc aaagaattga ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa
     9961 caaataaaac gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa aagaccaaga
    10021 aatttcttga tctttttgat atttatttga ctgctgtatg acgttgttcg aactgatgtt
    10081 gttttcgact taaccatgga agaagttgcg catgcagcac taaaaagaca gcactcacga
    10141 tgaatccttt gatcaagttg aaaggaacga tcccaatcag tacatagttt gctaaagaca
    10201 tgcccagcat ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc gtaataacga
    10261 aataatttgc gatactcata aaaatagtta gagcgatggt tcctgtaatg acacctaaaa
    10321 atttgtttct tcctttatgc tgtttcttta cactaaagaa ataataaatc gggaatgtaa
    10381 agatcgttgt tgataagaaa ctagcagtgt ctcctaccaa attatccgga gaaaaacctg
    10441 tagtcagcag atgtaatact gaacgcaaga acgctgttgc aactccggcc gctggaccga
    10501 ataagaacat gctgatcaat accgagatat cactaaaatc gatcttcaaa aaactaaaag
    10561 ccggcatgat cggaaaagca aaatactgta aaactaatgc gatcgcagca aacatcgcta
    10621 ctgcaaccat tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt aggactcctc
    10681 ttcagcgaac tttttgacgt ttctctccaa atgaaaaaac tctattttcc agggaaaata
    10741 gagttagagt tatttgatca agcgtcgtca aataagctct atcttcttta tccagactat
    10801 actgtcggta tcggaatttc accgattcag ctattttttt gcaaaatagg tcgtggacta
    10861 taaccaccgg tcgggaattt caccctgccc ctgaagacga accttatata atttttgttt
    10921 acatattcat tatacatatc tattcaaaaa acgcaacatc taacaattat aaaaagtaag
    10981 tgttcatttt gagtggttca gcaaactgct gacttctttt ttgttcagtt cacggtaatc
    11041 cccaggacgt aggccttgta aagtcagatc tccgtatttt tcacgtttta gtttttgcac
    11101 aggaagacca actgcttgaa gcatattttt cacttgatga ttacgtcctt cgtggatcgt
    11161 caattccacg atactgtggt ttgtcttagg atccactgat aaaatttgga atttagctgg
    11221 agatgttttt cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt
    11281 agcgatccct tttacctttg caacgtaaac tttatccact tcatgttttg gatgtgtcag
    11341 gcgctgagca aaatctccat catttgtcag tagcaagatc cccgaagtat cgtaatccag
    11401 tcgacctaca ggatagatac gttcttttac catcggcaaa agatccgtaa caacttttct
    11461 tcctttgtca tctgcaacag cagaaataac tcctttaggt ttgtacaata aatagtaacc
    11521 gtattcttct tgataaattg ggacttcatc cacttccaca gtgtcatgtt tcccaacttt
    11581 tatccctaat tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc
    11641 ttctgctttt cgacgagaag tgatgccagc gtgggcaatt actttctgca atctctccat
    11701 tacaattatt ctcctttatt cattccatca aagaatagat ctggtgacac gtcttcttca
    11761 agtgaaattt ccatttcttg tgtatcaggc agttcttcta atgatttcaa accaaaataa
    11821 tctaagaagt agccagttgt tccatataat atcggccgac ctggtccttc tactctacct
    11881 ttttcttcaa tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct
    11941 cgaatctgct cgatttcagc tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt
    12001 tcgacggcgg cttgagataa tgtgtttgat gtcccttgcg catatcgctt aagtaaggga
    12061 gccagctctt ttttagtcgt taatacataa tggctttccg tttcaaaaat atgcaaagct
    12121 gagtgcgagt tttgagtgta agattctttt aattctttca ctaaacttgt gacttctgct
    12181 tcgctgagat ttaacagtcc gcttaattct tcattactga tcccttcctc gccaacaata
    12241 aaaagaaggg cttcaagttc acttaatttc gtcatgttcg gtatctccta ctgctggata
    12301 aagcataatt gattcatagt tttctttttg ttgcgcaatg atctgccctt ttttcatcaa
    12361 ttctagcaaa gccataaaag tagtgacaac ttcactttta gaaggtgact caaaaaaaga
    12421 atcgaaagta cagccatgcg catttctttt tatacgctct tcgataaata ccattcgttc
    12481 ttcgatcgtt gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa atttcttacg
    12541 ttctaagatt ttctgcatgg cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa
    12601 cggcttttca gcatccgcta acccatcgat gttcataggt tcttttgtgt aataaagact
    12661 tcgttcctct gctttttctg tcagtctttc agctgcatat ttatatttac ggtattctaa
    12721 aagctgagtg accaattctt ctcgaggatc ttcttcccat aattcttcat cgtctgtgcc
    12781 gaattcttgg acaggaagca atgtcttgct tttgattgcc atcaatgtag cagccatcac
    12841 taagtattct cctgcaacag ctagctcgag tgttttcatt gtatggatat aattcatata
    12901 ttgctccgtg atcgtagcga tcggtatatc atatatatcg atttccattt gctgaatcag
    12961 atgaagcagc agatcgagag gaccctcgaa tacatctaat ttaatattga tctccgtcaa
    13021 ctcattcact ctttcttctt tttctttgct agccattttc caatgtaagg ctctgtgatt
    13081 tcagttgcac ggatggagac atccgggtac ttttcctgta attcatctaa catttgaaag
    13141 cccatattct caccgcgata agacggattc aaagaaatgt catggatgat cagctgatcg
    13201 ttcgttgccc agtaaatacc aataatccct tgaatgttgg ttgaaccttc tggcgtaaag
    13261 aaaaaaagtt cataatcgtc ttcagattcg tacgtatcaa tctccttcaa caaagaagtt
    13321 ttacctgtca gtttcttatg gaaactcaat agtcccatag caattttacg gttttctttc
    13381 cggtaatgtg ttaacatctt ctcaccttcc tatgactaag cccttggaaa atgctgctta
    13441 taaacatccg tcattctttt cttcgtgata tgtgtataaa tctgagtcgt agagatatcc
    13501 gcgtgaccta acaattcctg tactgtccgc aaatcagctc cattttctag cagatgtgtg
    13561 gcaaagctat gccgcagcgt atgtggtgtt acttctttgt aaatcccagc ctctcgtact
    13621 aactgcttca gatttttcca gattccttgc cgagaaagac ctttcccatg attattgaca
    13681 aatacatgca tttcagatgc gtcctttaca agtaatggac gtgcttcgtc taagtagcgt
    13741 tccagccatt ggatcgcata atcacctaaa ggaatgatcc gctctttatc tccttttcca
    13801 actgtctgta ccagtccaag agaaagatga agatctccca atttcaatcc aatcaattca
    13861 ctgacgcgca tcccagtagc atacatgact tccaagattg cacgatctct tatccctaac
    13921 gtttttgtcg tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt caatgtactt
    13981 ggtaatttct gtgctttctt cggcgtatcg atatgttgca tcggatcatg atcagtgata
    14041 cgctcttgcc gtaaaaactg atggaatctt ctgagacttg agatcatccg agtaacagtt
    14101 gctgtcgctt ttttttcttc atgcaacttc tccagaaaac ggatgactaa aaagcgatcg
    14161 acatcctgcc atgaagcaat tttctgctcc tgtagaaaat gcaagtactg ttccaaatca
    14221 cgttcatagc tttgtcgtgt attggcggac aatccccgtt caatattcaa gtagtggaga
    14281 taatcgttga tttgctcttt cataataatc cccctcatgc ctctctatca tatcagatac
    14341 cgcttcattc tgatagtctt tttccataat ctgagcaaag aaaaagccgt ctagacactc
    14401 agacggactg gtttcttttt tcggctctat aatttatggg actgatgaat cagaattgat
    14461 tcttcagccc cattttcatt ttaggtatta aa
//
LOCUS       DAYYDS010000062        14482 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000062 DAYYDS010000000
VERSION     DAYYDS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.063"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACHA02_001970"
     CDS             complement(287..649)
                     /locus_tag="ACHA02_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670291.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACHA02_001971"
     CDS             complement(723..1103)
                     /locus_tag="ACHA02_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8670292.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACHA02_001972"
     CDS             1185..2360
                     /locus_tag="ACHA02_001972"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8670293.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACHA02_001973"
     CDS             complement(2437..3021)
                     /locus_tag="ACHA02_001973"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8670294.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACHA02_001974"
     CDS             3222..4220
                     /locus_tag="ACHA02_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8670295.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACHA02_001975"
     CDS             4332..4841
                     /locus_tag="ACHA02_001975"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8670296.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="ACHA02_001976"
     CDS             complement(4871..5530)
                     /locus_tag="ACHA02_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8670297.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="ACHA02_001977"
     CDS             complement(5532..6296)
                     /locus_tag="ACHA02_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8670298.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="ACHA02_001978"
     CDS             complement(6304..6981)
                     /locus_tag="ACHA02_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8670299.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="ACHA02_001979"
     CDS             complement(7018..8403)
                     /locus_tag="ACHA02_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8670300.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="ACHA02_001980"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHA02_001980"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACHA02_001981"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACHA02_001981"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8670301.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="ACHA02_001982"
     CDS             9342..10199
                     /locus_tag="ACHA02_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670302.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="ACHA02_001983"
     CDS             complement(10280..10615)
                     /locus_tag="ACHA02_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670303.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="ACHA02_001984"
     CDS             complement(10682..12052)
                     /locus_tag="ACHA02_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8670304.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="ACHA02_001985"
     CDS             complement(12130..12666)
                     /locus_tag="ACHA02_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8670305.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="ACHA02_001986"
     CDS             12975..14456
                     /locus_tag="ACHA02_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670306.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4461 a         2883 c         2353 g         4785 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta tt
//
LOCUS       DAYYDS010000063        14342 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000063 DAYYDS010000000
VERSION     DAYYDS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.064"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..81
                     /locus_tag="ACHA02_001987"
     CDS             <1..81
                     /locus_tag="ACHA02_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFM8670307.1"
                     /translation="DTIVLHQRKLDLLKEQKKGFLQKMFV"
     gene            108..443
                     /locus_tag="ACHA02_001988"
     CDS             108..443
                     /locus_tag="ACHA02_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670308.1"
                     /translation="MKFLNFLNYKYLNWVLYGITLFFIIYYFFTDDDIFLILAILFLV
                     SSNFSDNLIEVLTEPEIRSMTDEEQQIIKAMISENKKDVVVIKKIREYTNLGVLQAKK
                     VYEKKKKGL"
     gene            620..1570
                     /locus_tag="ACHA02_001989"
     CDS             620..1570
                     /locus_tag="ACHA02_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8670309.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2088..2498
                     /locus_tag="ACHA02_001990"
                     /pseudo
     CDS             2088..2498
                     /locus_tag="ACHA02_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2941..3753
                     /locus_tag="ACHA02_001991"
                     /pseudo
     CDS             2941..3753
                     /locus_tag="ACHA02_001991"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3748..3912
                     /locus_tag="ACHA02_001992"
     CDS             3748..3912
                     /locus_tag="ACHA02_001992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670310.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            3852..3974
                     /locus_tag="ACHA02_001993"
                     /pseudo
     CDS             3852..3974
                     /locus_tag="ACHA02_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(4536..5042)
                     /locus_tag="ACHA02_001994"
     CDS             complement(4536..5042)
                     /locus_tag="ACHA02_001994"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8670311.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RYWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRNSKILVPINILKSKISSLVSPLTEEL
                     QKIAMKVM"
     gene            complement(5047..5814)
                     /locus_tag="ACHA02_001995"
     CDS             complement(5047..5814)
                     /locus_tag="ACHA02_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8670312.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6304..6729
                     /gene="lacA"
                     /locus_tag="ACHA02_001996"
     CDS             6304..6729
                     /gene="lacA"
                     /locus_tag="ACHA02_001996"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFM8670313.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6746..7261
                     /gene="lacB"
                     /locus_tag="ACHA02_001997"
     CDS             6746..7261
                     /gene="lacB"
                     /locus_tag="ACHA02_001997"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFM8670314.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7272..8204
                     /gene="lacC"
                     /locus_tag="ACHA02_001998"
     CDS             7272..8204
                     /gene="lacC"
                     /locus_tag="ACHA02_001998"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFM8670315.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8207..9187
                     /gene="lacD"
                     /locus_tag="ACHA02_001999"
     CDS             8207..9187
                     /gene="lacD"
                     /locus_tag="ACHA02_001999"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8670316.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9215..9532
                     /locus_tag="ACHA02_002000"
     CDS             9215..9532
                     /locus_tag="ACHA02_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8670317.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9532..11238
                     /locus_tag="ACHA02_002001"
     CDS             9532..11238
                     /locus_tag="ACHA02_002001"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8670318.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11380..12786
                     /gene="lacG"
                     /locus_tag="ACHA02_002002"
     CDS             11380..12786
                     /gene="lacG"
                     /locus_tag="ACHA02_002002"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8670319.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12809..13081
                     /locus_tag="ACHA02_002003"
     CDS             12809..13081
                     /locus_tag="ACHA02_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFM8670320.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13102..14001
                     /locus_tag="ACHA02_002004"
     CDS             13102..14001
                     /locus_tag="ACHA02_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8670321.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4685 a         2363 c         2765 g         4529 t
ORIGIN      
        1 gacactatcg ttcttcatca acgtaagtta gatttgttga aagagcagaa aaaaggattt
       61 ctacaaaaaa tgtttgtttg atattttaaa tagaaaagag agataaagtg aagtttttaa
      121 attttttgaa ctataaatat ttaaactggg tattatatgg tattacatta ttttttatta
      181 tttattactt ttttaccgat gatgatattt ttttaatttt agcaatactt tttcttgttt
      241 catctaattt tagtgataac ttaattgaag ttcttacaga accagaaatt cggtctatga
      301 cggatgaaga acaacagatt attaaagcta tgatttcgga aaataagaaa gacgtagtag
      361 ttatcaaaaa gattagagag tatacaaatt taggtgtttt acaagccaaa aaagtatatg
      421 aaaaaaaaaa aaagggtcta taattacaaa ttagataata cattagaagc tggagacaag
      481 attgtctaga taatgtgtga gataaaattt ttggcaggtt atcatacata agacaaaatg
      541 tttttaaaac tgataacgta tttattccac aacaattacg catttatttc tgagaaaaat
      601 ataaagcagg aggattacaa tgctgaaaac ttattcctta acaaaagata cattattagt
      661 ttcgtcagaa tcaaatatca aaaattcttc tgttgtttat gtttatggtg acgaacgaga
      721 atatatcgaa tcttctgaac ataaatatga ctttaaaata ggcaatatac taacgtttga
      781 tgatcgagtt gcatatgaca cgatgataga taagaacgat gaaaaaagtt tgctcgtaag
      841 ttttttgttt cccgaggtac atgaaggtgg gcatctagat accctgacga catctgttgt
      901 tttcttggta tttaaaaata agttgataat ttttacaaaa caaaaattaa aattcattcc
      961 cgaactctta tccaatatgg ttttacgtga tgtcaatcat ttcgtggagg aagtactttt
     1021 aaagatcatg caaaaagatt tctatatgat gcttaacgac cttcgtggag tcaaggaaaa
     1081 aattgatgat ttagactcag aaatatcaac gtccggttca ctcaaaccaa cgtttggtga
     1141 tttattgact cttcaaaaat acatgatagc tttgtcaaca acttataaag ctaaccataa
     1201 agcgcttgat ttcattaaaa aaaatttcac aactattgac gatattgatt ttagtactaa
     1261 aaaaataatt gatgatttat acgatacaag tgatacaatg gatagaatca ttttaagtta
     1321 cagccaatat ttggacaaat tggaaaatat gataaacaat atgatttcgt atcagctaaa
     1381 catgattatg aaaaccctaa ccgagatatc aattgttttg actattccag caattatttt
     1441 tggcttttgg ggaatcaatg tccatgtccc gtttgaaaag tcttcttatg gtgttttagc
     1501 cgtattgatt atctctgcta tactaagtct aatttgctgg cttttgatgc gacgcaaaac
     1561 atatttgtaa agtatttgtg ataccctaat atgatttaaa gcagaatttc tttgttgact
     1621 ttttaaaagg ctgattcctt tcaataattt aatagcttca gcggctgctc agaagtcaat
     1681 aaattggact atttttattc agttacttag gcaacatttg agatacgatt gcgattataa
     1741 aaattgatat accaaccaat ggctgcttgg aagactgaca tattagcctc gatatcatcg
     1801 aataactaaa tacagaaaaa tacaataaat attatcaaac tagggactat actagaaaac
     1861 atcagtacat tccttaactc agggtctata attagaaaat cgcccctttg aaaactgatt
     1921 aaaacagccc ccattatcta gtgactagat aatgggggct gtttttttgt taagtgatat
     1981 aataaaccat aaaagaagtt tagaaatacg gtcctggtca tcaataaata aacaatttaa
     2041 ttaattattt atttaattat ttaatttcag aaaggtatac atgaatcatg gtgcaacaaa
     2101 ttgtcctacc catcaaagac tccagcgtct taaagatggt acaagataca cttctcgata
     2161 gtttccgtgc tggtcgccga aattacaccg tttttcaagt tggtaaggcc acgctactac
     2221 gtgtgagtga tgtcatgaca ttaaaaaaat cagatgtcta taatccagac ggctctgtca
     2281 aaaatgcaac ctttattcat gataaaaaga ccggtaaagc aaacacgtta tatttaaagc
     2341 ctgttcagca agacttgttg caatatcatg attggctggc ccaagaaaat atcaattctg
     2401 agtggttatt tccctcgaca gcccattatg atcttcatat cactgagaag cagtttcata
     2461 aggtgatggc gcatgttggt gatctcttag gaatcaactt ggaagacaaa gagaaaaaat
     2521 tgcgagtcag aaaaccgttt tgagatatga acagcatttg ccttcacgcc ttagcgattt
     2581 taaataatag ccttgaaagt gattatggaa aagccctcta cgctcaatgc aaaattgacg
     2641 tagaaactat ttttgatcga ttgaaaggtg tatttggtat gtgaagaacc catgtcagag
     2701 gcaagaacgt tgcacctact gacattggaa tgatgcttat gagcatgaat ctaaccaaat
     2761 tatcccttga ggctagaaga aaagcggaag cttttcaaca caaagcagcc aaaaataaaa
     2821 aacgcgacga agcaatcaca tttatgatta ttcatcgcgt tttttatatt tagagctagt
     2881 tatttgctag acccactatt tacagatgaa acgtttatca cctttggttt tgggcattta
     2941 gtggaattag cagaaccagg tcattatgac gaaaagtggc aaaattggaa acttgaatct
     3001 ttgccgattt ttcctgatcg atacgatttt gaagttgcaa aagataaggg aaagcagttt
     3061 aaaattgttg cagaacttct caaaaaggca aatacaatta ttgttgcaac agatagcgac
     3121 agagaaggtg aaaatatcgc ctggtcgatt atccataatg caaatgcctt ttcaaaagat
     3181 aaaacattta aaagactatg gatcaatagc ttagaaaaag atgtgatccg aagcggtttt
     3241 caaaatttgc aaccaggaat gaattactat cccttttatc aagaagcgca aacacgccaa
     3301 attgccgatt ggttgatcgg catgaacgca agccctttgt atacgttaaa tttacaacag
     3361 aagggcgtac aaggtacatt ttcactagga cgtgttcaaa cgcccacctt ataccttatt
     3421 tttcagcgcc aggaagccat agagaatttt aaaaaagaac cttttttcga ggtggaagct
     3481 aagtaaaaca agggtcattt aagggcgttc taagccccac acagcgtttt aaaacccaag
     3541 aggagctttt tagcttttgt ttcttctaaa caagctaaaa taggcaatca agaggggata
     3601 attgctgatg ttcaaaccaa agagaagaaa acgaatagtc cgagttcgtt ttctttaagt
     3661 agtttgcaat caaaagtcaa tcagctttat aaagcgacag cgagccaaac tttaaaagct
     3721 atgcaaggac tgtatgaagc aaaattattg agttatccaa gaacagatac accatttatt
     3781 acagagaacg aatttgctta tttaaaagcg aattttggca aatatagcgg ttttttagga
     3841 cttgatcttg aaatgttgtt atcattgata atgatacgtt caaacaacag cacccaaatt
     3901 ttgacgaatt agtgaaactc tatgaaaaag acgtagtaaa acatgcaaca tcctattcta
     3961 atcagctggt aaagcttaat taggttatat aagcttgaaa ataaagggtt tgtccaatat
     4021 ggacaagcct ttttcttatg ttttgccaca gatttgccac acttattgtg taagagtatc
     4081 aaggataggt ataatttgat actttgtttt tttagtgatg tgcaaataaa tttctgttac
     4141 acgactacct ctagaatgcc ctacacggtc ttgaattgct tctaggggta ttccttactc
     4201 tgctaagaaa ctaatatgcg tatgacggaa tatatgggtc gtaatatgtt tattaaaact
     4261 aagtttatta tcaatatcaa ttcctgctcg ttttggatta ccttttcgat ttaattttcc
     4321 attttcaggt gagttgagga aattgttaag gacactataa tgaagaggtg ttccattata
     4381 gtcgggttta tgacgacttt tggaagtaaa aatccatcca ttatctttat aatcaggatt
     4441 gaatctcata tgttggtgat ttaactcaat ttcctcttta agaatttcta tagccctttt
     4501 agataattgg acatttcggt aactttgtaa gttttttaca ttaccttcat agctattttc
     4561 tgcagctcct ccgtgagtgg actgactaat gatgatattt tgcttttcaa aatattgata
     4621 ggcaccaata ttttggagtt tcgactctgt aagtcaacag ggccattagt tagaatacct
     4681 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
     4741 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
     4801 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
     4861 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaatatct gaaacgtttg
     4921 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
     4981 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
     5041 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
     5101 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
     5161 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
     5221 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
     5281 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     5341 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     5401 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     5461 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc caggtccaat
     5521 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     5581 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     5641 caattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     5701 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     5761 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     5821 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     5881 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     5941 ataagaacag aggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     6001 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     6061 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     6121 gttatctatt ttttaaaaaa acaaataaaa aaaacaaaaa ataaacaaaa aaagtgttgc
     6181 tttttgtttg aatgcttgat attatataaa caaagaaatg atgaaaacgt tttttcgaac
     6241 attttgcaaa atgttttcta cttctacgta gcatttcttt ctaaaattta ggaggtggtc
     6301 aaaatggcta ttgttgttgg tgcagatctc aaaggtacga gattaaaaga tgttgtgaaa
     6361 aattttcttg tagaagaagg ttttgaggta attgatgtga ctaaggacgg acaagatttt
     6421 gttgatgtta ccttggcagt tgcctctgaa gtaaataaag atgagcaaaa cttaggtatt
     6481 gtaattgatg cttatggagc tggtccattc atggtagcaa ctaaaattaa aggtatggtt
     6541 gcagcagaag tttcagatga acgctcagct tatatgacac gtggacataa caattcacga
     6601 atgattacgg ttggcgctga aatcgtcggt gatgagcttg ctaaaaacat cgctaaaggt
     6661 ttcgtcaatg gtaaatatga tggcggacgt catcaaatcc gagtagatat gcttaacaaa
     6721 atgtgttaag aaaaggagtt aaggaatgag aatcgcaatt ggatgtgacc acattgtcac
     6781 agatgaaaaa atggcagtat cagaattctt gaaatcaaaa ggacatgagg tccttgattt
     6841 cgggacatat gaccacgcac gtactcacta ccctatctat ggtaaaaaag tcggtgaagc
     6901 agtagtaagt ggtcaagcag acttgggagt atgtatctgt ggtacaggtg tcggcattaa
     6961 caatgcagtc aataaagttc caggtgttcg ctcagcgttg gttcgtgata tgacatcagc
     7021 tatatatgca aaagaagagt taaatgcgaa tgttatcggt ttcggtggaa agattactgg
     7081 tggtcttctt atgaatgaca tcattgaagc tttcattgaa gctgagtaca aaccaacaga
     7141 agaaaataaa aaattgattg caaaaatcga acacgttgaa acacataatg cacatcaagc
     7201 agatgaagat ttctttacag aattccttga aagatgggac cgtggtgact accacgatta
     7261 aggtataaca aatgattctg acagtcacac taaatccttc agtagacatc tcttatcctc
     7321 tagaaacatt gaaaattgat acagtgaata gggtgaaaga tgttagtaag acagctggtg
     7381 gaaaaggctt aaatgttact agagtcttgt atgaatctgg tgataaagtt actgctacag
     7441 gtttcttagg tggtaaaatt ggtgaattta ttgaaagcga attagaacaa tctcctgtta
     7501 gtccagcttt ttataaaatc tcaggaaaca ctagaaactg tattgctatc ttacacgagg
     7561 gtaaccaaac agaaattttg gaacaaggac caacaatttc tcatgaagaa gcagaaggct
     7621 ttcttgacca ctatagcaat cttattaaac aatcagaagt tgtgacaatt tcaggaagtt
     7681 taccttcagg acttcccaac gattattatg aaaaacttat ccaacttgct tcggatgaag
     7741 gagtagcagt cgttttggat tgttcaggtg cacctcttga aacagttcta aaatcgtcgg
     7801 ctaaacctac tgccatcaaa cctaataatg aggaactttc acagttgctt ggaaaagaag
     7861 taacaaaaga tattgaagaa ctcaaagacg ttcttaaaga gtcacttttt agtggtatcg
     7921 aatggatagt ggtctcatta ggtcgaaacg gtgcctttgc aaaacatggt gatgttttct
     7981 ataaggtaga tattcctgat atcccagttg ttaatccagt cggatcaggg gactcaacgg
     8041 tagctggtat tgcatcagct ttaaatagta aaaaaagtga cgctgactta ttaaaacatg
     8101 cgatgacatt gggtatgtta aatgctcaag aaacaatgac agggcatgtg aatatgacta
     8161 attacgaaac actaaactca caaattggag taaaagaggt ataaaaatgg tacttacaga
     8221 acagaaacgc aaatctttgg aaaaaatttc agataaaaac ggtgttatct cagctttggc
     8281 atttgaccaa cgtggtgctt tgaaacgttt gatggcacag tatcaagata cagaaccaac
     8341 tgtagctcaa atggaagaac taaaggtttt ggttgctgat gaacttacaa aatacgcttc
     8401 ttcaatgttg cttgaccctg aatatggact tccagcaaca aaagcattgg ataaagaagc
     8461 aggtcttctt cttgcctatg aaaaaacagg ttatgataca tcaagtacaa aacgtttgcc
     8521 tgactgcttg gatgtttggt ctgcaaaacg tatcaaagag caaggcgctg atgccgttaa
     8581 gttcttgctt tactatgatg tagacagctc agatgaactt aaccaacaaa aacaagctta
     8641 tattgaacgt gttggttctg aatgtgtcgc tgaagatatt cctttcttct tggaaatcct
     8701 tgcttatgat gaagaaatct cagatgcagg ttctgttgaa tatgcaaaag tgaaacctcg
     8761 caaagtcatt gaagcgatga aagttttctc tgatccacgc tttaacattg atgttcttaa
     8821 agtagaagta ccagttaacg ttaaatatgt cgaaggtttt gctgatggtg aagtagttta
     8881 cagtaaagct gaagctgctg acttctttaa agcacaagaa gaagcgacta accttccata
     8941 catctaccta agtgcaggtg tatctgctaa attgttccaa gaaacattgc aatttgctca
     9001 cgactcaggt gccaagttta atggtgtgct ttgtggacgt gctacttggg caggatctgt
     9061 agaaccttac atcaaagaag gtgaaaaagc agcacgtgaa tggttgcgta ctactggatt
     9121 cgaaaatatt gatgaactta acaaagttct tgttaaaaca gctagtccat ggactgataa
     9181 agtatagtaa aaacataaaa cggaggatat tgttatgaac agagaagaga tgactctctt
     9241 agggtttgaa attgttgctt atgctggaga tgctcgctct aaacttttag aagcgcttaa
     9301 agcggctgaa aatggtgatt tcgctaaggc agatagtctt gtagtagaag caggaagctg
     9361 tattgcagag gctcacaatt ctcagacagc tatgttggct cgagaagctt ctggggagga
     9421 acttccatac agcgttacca tgatgcatgg tcaggaccac ttaatgacta ctgtcttatt
     9481 aaaagatgtg attcatcacc tcatcgaact ttataaaaga ggagcaaaat aatgcataaa
     9541 ctcattgaac ttattgagaa agggaaaccg ttctttgaga aaatctctcg aaacatctat
     9601 cttcgtgcta ttcgtgatgg atttatcgct ggtatgccag tcatcctttt ctcatctatc
     9661 tttatcctta ttgcctatgt cccaaatgct tggggattcc actggtcaaa agacattgag
     9721 accttcttga tgactcctta tagctattcg atgggtatcc tagcattctt tgttggtggt
     9781 actaccgcta aagctttgac ggactcaatg aaccgtgacc tacctgcgac caatcagatt
     9841 aacttcttat ctacgatgct agcgtctatg gtagggttcc ttttgatggc agctgagcct
     9901 gcaaaagaag gaggcttctt gacagccttc acgggaacaa aaggtctttt gacagcattt
     9961 attgcagcct ttgtgacagt taatgtttac aaggtctgtg tgaaaaataa tgttactatt
    10021 cgtatgcctg aagaggttcc accaaatatc tctcaagtat ttaaagactt gattccattc
    10081 actgtagcgg ttgttctcct ttatggattt gaactcattg ttaagggaac tcttggtgta
    10141 accgttgcag aatcaatcgg tacccttctt gctcctctct tctcagctgc agatggttac
    10201 cttggaatta cacttatctt tggtgcttat gccttcttct ggtttgttgg tatccacggt
    10261 ccttcaattg tcgaaccagc aatcgctgct atcacttatg ccaatatcga tgccaacttg
    10321 catcttatcc aagctggaca acatgcagac aaagttatca cttctggtac tcaaatgttt
    10381 attgttacca tgggtggaac aggagctaca ttgattgttc cattcttgtt catgtggatt
    10441 tgtaaatcag aacgtaaccg tgccatcgga cgtgcctcag ttgttccaac attctttggg
    10501 gttaatgagc caatcttatt tggtgcacca atcgtattga atccgatttt ctttgtaccg
    10561 ttcattttcg ctccgattgt caacgtttgg atctttaaat tctttgttga cacattgaac
    10621 atgaactcat tctctgccaa ccttccatgg gttactcctg gtccgttggg aattgtactc
    10681 ggtactaact tccaagttct atcatttatc ttagccggac tcttggtagt tgttgatact
    10741 atcatttatt acccatttgt taaggtatac gatgaacaaa ttcttgaaga agaacgttct
    10801 ggtaaaacaa acgatgctct taaagaaaaa gtagctgcaa acttcaatac tgctaaagca
    10861 gatgccgttc ttggaaaagc aggcgttgct aaagaagatg tagcagcaaa caataatatc
    10921 acaaaagaaa ctaatgtcct tgttctttgt gcaggtggag gtacaagtgg cttgcttgcc
    10981 aatgccttga acaaagcagc agcagaatac aatgttccag tcaaagcggc agcgggtagt
    11041 tacggtgctc accgtgaaat gttgccagag tttgacttgg ttatcttggc tccacaggtc
    11101 gcttcaaact tcgatgatat gaaggctgaa acggataaat tgggcattaa acttgcaaaa
    11161 actgaaggag ctcaatatat caaattgaca cgtgatggac aaggtgctct tgcatttgtt
    11221 caacaacagt ttgattaata aatttgtaaa agttatttga gacgagatta ttctttcaat
    11281 tccttataat attgttaacc atatcattgc atttcatttc cttggtttcc ggaactcaga
    11341 atatcgtctc aaatgctttt tttgaaagga tttatagtta tgactaaaac acttcctaaa
    11401 gattttattt ttggtggtgc tacagctgct tatcaagcag aaggtgcgac tcacacggat
    11461 ggtaaaggcc cagttgcatg ggataagtat cttgaagaca attattggta cacagcagag
    11521 cctgcaagtg atttttacca taaatatcct gttgatttag aattagctga aaaatatggc
    11581 gttaatggta ttcgtatttc aattgcctgg tctcgtattt tcccaactgg ctatggcgaa
    11641 gtaaatgaaa aaggtgttga attctaccat aatctttttg ccgagtgcca caaacgtcat
    11701 gttgagccat tcgtgacttt gcaccacttt gacacaccag aagttcttca ctcagatgga
    11761 gatttcttaa accgcgaaaa tatagaacac tttgtagatt atgccgcttt ctgttttgaa
    11821 gagttcccag aagtaaacta ctggacaact ttcaatgaaa ttggcccaat tggagatggt
    11881 caatacttgg ttggtaaatt ccctccaggc attcaatatg atcttgccaa agtcttccaa
    11941 tcacaccaca atatgatggt ttctcatgca cgtgctgtga aattgtataa agataaaggt
    12001 tataaaggcg aaattggtgt agtacatgcg ttgccaacca aataccctta tgatccagaa
    12061 aatccagcag atgttcgtgc cgctgagtta gaagatatca tccataacaa atttatcttg
    12121 gatgcaacct accttggaca ctattcagat aaaacgatgg aaggggtcaa ccacatccta
    12181 gctgaaaatg gtggagaact tgatcttcgt gatgaagact tccaagcact tgaagcagct
    12241 aaagatttga acgatttcct tggtatcaac tactacatga gtgattggat gcaagccttt
    12301 gatggtgaga ctgaaatcat tcacaatggt aagggtgaaa aaggaagttc taagtaccag
    12361 attaaaggtg ttggtcgccg aatcgcacca gattatgtgc ctagaacgga ttgggactgg
    12421 attatttatc ctgaaggctt gtatgaccaa atcatgcgag tgaaaaatga ttatccaaac
    12481 tacaagaaga tttacatcac tgaaaacggt cttggataca aagatgagtt tgtagataat
    12541 accgtttatg atgatggtcg tattgattac gtgaagaaac acttggaagt tttatcagac
    12601 gcaattgcgg atggtgcaaa tgttaaaggt tacttcatct ggtcacttat ggatgtcttc
    12661 tcatggtcaa acggatacga aaaacgttat ggactattct atgtagactt tgacacgcaa
    12721 gaacgctatc ctaagaaatc agctcattgg tataagaaag tagcagaaac tcaagtgata
    12781 gagtaatatt acttgaaaat gtaaaaaatt gttatccgat actttagaaa aagtacaaca
    12841 cttatttaga ccaggctttg aaaaggtata tattgttagt ttcgaagatt gtcctatgat
    12901 tcctgagctt gaagctaccc cgttgctaaa atgtggtaaa tggtatgttt caactggaaa
    12961 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
    13021 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
    13081 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
    13141 tttaaaacac ttggtggtca actgacttcg attaaagaca aggatgggat agagtatctc
    13201 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
    13261 agtttacgaa atgattgggc tatttataga cctcaagaaa gacccttttt tacaggtctt
    13321 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
    13381 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
    13441 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
    13501 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaatagt
    13561 cctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
    13621 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
    13681 tttttggaaa atcaaaaaat tctcgatttg gactactcac ttttttccca tgatgccatt
    13741 acacttgacc agctcaagtc tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt
    13801 cttcgagtag attttgatga ttttccgaac ttgattctgt ggtctacaac taataaaagt
    13861 ccattcattg ctttggaacc ttggagtggt ctctccactt cacttgaaga aggtaatttc
    13921 ttagaagata aaagacaggt aacgaaaatt tcacctcaag aaacttctag aaaaatctat
    13981 gatattacta tccttaatta aaatttgtta ttatttgagt cttcaataag atttgaatac
    14041 tcttaccttg tgatgataga ttggtcttct ccgtgccagt cttacatcgc aaggttttta
    14101 agttttttgt gggtctatgt caactgtagt gggtgacgaa aagctaatat ctaaagacag
    14161 tctggtagta aaaaataaga ttatttacta cttcttgatt taaaataata gtggagccat
    14221 taatttgtgg caaatttaat ttaatatttt gcctcaaatt agccacactt tttatagaac
    14281 taagaggaaa aatgaaattt tcttcctatt aatatagttg tttgaaatct taaatatagc
    14341 gt
//
LOCUS       DAYYDS010000064        13997 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000064 DAYYDS010000000
VERSION     DAYYDS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.065"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..2288
                     /gene="acm"
                     /locus_tag="ACHA02_002005"
     CDS             123..2288
                     /gene="acm"
                     /locus_tag="ACHA02_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8670322.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2560..3045)
                     /locus_tag="ACHA02_002006"
     CDS             complement(2560..3045)
                     /locus_tag="ACHA02_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8670323.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3135..3480
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3525..4166
                     /locus_tag="ACHA02_002007"
     CDS             3525..4166
                     /locus_tag="ACHA02_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8670324.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4185..4820
                     /locus_tag="ACHA02_002008"
     CDS             4185..4820
                     /locus_tag="ACHA02_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8670325.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4838..5653
                     /locus_tag="ACHA02_002009"
     CDS             4838..5653
                     /locus_tag="ACHA02_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8670326.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5972..7408
                     /locus_tag="ACHA02_002010"
     CDS             5972..7408
                     /locus_tag="ACHA02_002010"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8670327.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7597..10044
                     /locus_tag="ACHA02_002011"
     CDS             7597..10044
                     /locus_tag="ACHA02_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8670328.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10274..11341
                     /gene="rlmN"
                     /locus_tag="ACHA02_002012"
     CDS             10274..11341
                     /gene="rlmN"
                     /locus_tag="ACHA02_002012"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8670329.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11331..11537
                     /locus_tag="ACHA02_002013"
     CDS             11331..11537
                     /locus_tag="ACHA02_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670330.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11534..12637)
                     /locus_tag="ACHA02_002014"
     CDS             complement(11534..12637)
                     /locus_tag="ACHA02_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8670331.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13190..13738
                     /gene="aac(6')"
                     /locus_tag="ACHA02_002015"
     CDS             13190..13738
                     /gene="aac(6')"
                     /locus_tag="ACHA02_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8670332.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4795 a         2317 c         2967 g         3918 t
ORIGIN      
        1 aaaataacta tcaatttttt agttaaaaga ttggaaatga gtgggggagg aattgaagaa
       61 aggatttttg agagatgata tagtaggttt tatttttagt taaaataggg gagatagaaa
      121 aaatgaaaaa gtgtagaaat attattttat caatgttatt tattattact aatataacat
      181 cactgattcc tgttcatgtc tatgcggatg caggcagaga tatcagcagt aatgtcactt
      241 cgttgaccgt ggacccaacg aatattacag atggaggaaa cataaaagta aaattctcgt
      301 ttgacgaaaa aaaacaaaat atccaaccag gtgattatct ttggatcaat tggccaagtg
      361 aaggaaatat tcgcggtgaa ggattccaaa aagaaatccc attaatgatt gaaaataaaa
      421 atgttggaac tttaacggtc agaaaagatt ctgcgcaggt tgtgtttaat gaaaatatta
      481 aaaacctaga ctcagtagaa ggttgggggc aatttgagat acaggccaga aacgtaaccg
      541 ataccggtga agaaaatacg ggaccattca ccgtgacgag cggtgataaa acagctactg
      601 taaatgtgac aaagccagct tctggttctt cttctagcgt tttttattat aaaacaggag
      661 acatgctacc tgaagatact aagcacattc gatggttttt gaatattaac aataacggca
      721 cttatgttga acaaccagtt aaaatcagtg atgaaatcca aagtggacaa agattagatc
      781 caagtacgtt tgaaattaat caaatacact taggtgaaca gaaggtttat agaggagaag
      841 agggaatcca acaattttta caagatttcc ccagcgctac atttaatttt tcagtaactg
      901 ataattacat tgaaataact ataccaaaaa actttgtcaa tcttaggaaa ataatggtga
      961 gttataagac gatcatagaa aacccagaac aaataaattt tgagaatcat tcggaagctt
     1021 ggtttaaaga atttaataaa cctgctgttg atggagaaag tttcaatcat acagttaaaa
     1081 atatttcagc ttctggtgga gtcaacggta cagttagagg cgaattgaag attttcaaat
     1141 atatcaatga cacagagatt ggtatcccaa atgtgacttt cgaattaaga cgagcagatg
     1201 aacagcctat tcaaggacag tctagtatcc tgttgacttc gaatgaacag ggagaagcta
     1261 gtatcaaagg cctgcaagta ggagactatg ttgtaaaaga aaaagaagca cccaattgga
     1321 ttgattttga tccactaagt tcaaatgaac tcaaattctc cataaacgaa aatgatacag
     1381 agggagtcag tttacctatt tataacaaga aaaaggttac gaacatcaca gcaactaaaa
     1441 tttggaacgg tggaaccact ccaagaccga gtatttattt taaattattc agagcaagta
     1501 caaacaattg ggaaccagtt cctgatgcag aaacaaaacg attagacaat ggaataactt
     1561 cagtgacttg ggaagatatc caacagtatg atgatagtgg aaacgaatat acgtttaaag
     1621 tgcaagaagt agatcaaaac ggaaatgatt atgtcccttc aggttatcaa aaaattgaaa
     1681 acgggctagt cgttacaaat gagaataaag aagtcatttc agtctcagga caaaaaacat
     1741 gggaagacaa tgagaaccaa gacggcaaac gaccgacgac gatcacagta aatctattag
     1801 ccgacggtca accgatccaa cacaaagaag taagtgaaaa agatgattgg cgctatcgtt
     1861 tcacaaatct gccaaaatac agagacgggc aagaaatcat ctatactgta acggaagaca
     1921 acgtgcccga atacagtacg acgattgacg ggtataacat caaaaacagt tatacaccgg
     1981 aaaaaacaaa tatatctata ccaggtagcc atataacacc gtggaaaaat tttccagaga
     2041 agacagagag agagaaaatt agaaatctat tctctcaaaa attgcaaata tttgctcata
     2101 caggagaatc cagcgaacaa agaagcaatt atattagtaa gagagctgta cctaaacaga
     2161 taacaaataa atcaaaaagc attcttccta aaacaagtag taaaaaatct agttttgctg
     2221 taatcattgg tttattgata gtaactatct gtgctgggat attcttacaa aaatataaaa
     2281 aagtttagtt agaatataat attttttgtt aaatatgact gaaatcaaaa gaataaaatt
     2341 ccgatttata atcgaaaaaa gaagcatttt tatatatgct tcttttttcg tagaatcaat
     2401 aaatatggat ttggctaact atggaagaat actcgtatta gttttcagcg ttcatactag
     2461 ttgttacatt gtttgattgg ccaaaagaat ttctgatata ttttgtgcta aataaagaaa
     2521 aagacgcttg aattgggaaa taagaaaact actctatcat tacaaatttt cataaactgg
     2581 cagatgttct aagtaaacaa gtatcttttc aaagtcggaa aaatccataa tcaacgtagt
     2641 ggtattgata tttggatgaa agcctacggt atcttgacga tcaatagcag catcgatcac
     2701 gacttggatt tttttctctt tgtcgtgcat caatgctaat ggagtcactg tacctgctgg
     2761 aacgcctagg aggtcaggaa gctgttcttc agaaacaaaa gataagcgct tttcttttaa
     2821 ttgttcagct agttgtttta aatcggcgtg cttttcatca ggcaagatga caagatagat
     2881 ttgttttcct tttcttgatt tcaataatag attttttact tgcggaccag gtagggaaaa
     2941 agggacattt ttgactgacg taatcgcagg atgatcgact ttttgaaaag agatacccag
     3001 tttttttagt agttcataag cttcttgttc ggttgcacga ctcatcgcgc atcaccctcc
     3061 tttttttatt agtgtatcat aaaaaagagt tgacaaatga aaaataatca tgtaaattac
     3121 acgtaaagtt aaaacaaatg acatcgaaaa gaagagtagc cgttaggtgt ttttagagag
     3181 tctctggttg gtggaaagag gcagcagtta accgtgaaac acatcttgga gtcgattttc
     3241 tgaagcgtat agtaggaaaa tccggggagc ccgttacagc tgcggtcgtt gctagatgac
     3301 cagataaggc tggcttttcc gtgaggagtc ggtaaaaaaa ggtggaacca cgtgattaac
     3361 gtcctttgat cttcggatca aaggactttt tgttttcatc tgaacggcaa cgactgtgtg
     3421 aggttttttc tgcaaggaaa aaataaacag aggtggaacc acgtataata cgtcctcttg
     3481 gcatttgcca agaggatttt tttattttaa aggaggaaaa gaagatggag tatttgattg
     3541 aagttatgcc gcaattatta gtaggagcac tcacaacatt gaaattattt ttatttacct
     3601 tgattggctc attaccatta gggattgttt tagcattagg attgtctttg cattttaagc
     3661 cgctacgcta cttgcttcaa acatatgtat gggttatgcg gggaacaccg ctgttgttgc
     3721 aactgatttt tattttttat gggttgccga cgattgggat cgtttttgat cgtttcgaag
     3781 cagcactcat tgcctttatc ttaaactatg cagcttattt tgcagagatc tttcgtggag
     3841 ggatacaatc gattcctcaa ggtcaatatg aagcagcgca agttttgcaa ttaagtccct
     3901 ttcaaacggt gacgaaaatc gtcatcccac aagtgacaaa gattgtttta ccttctgtag
     3961 ggaatgaagt tatcaacttg gtcaaagatt cttccttgat ctatatatta ggtttaggtg
     4021 acgtaatgcg tgctgggaaa atcgcgatgg aacgcgacgc gactttactg cctttgctag
     4081 gcgtggcaat catctatctt acattgactg gtattctaac aatcataatg aaacaagttg
     4141 aaggaaaatt aaattattac cgctaactga taggaggaag aaatatgatt gaattgaaga
     4201 aagtgaacaa agcatttggt gaaaaaaaaa taatcaaaga tttgaatcta atcattccag
     4261 atgggaaaac attagctatc gtaggtcctt ccggtggcgg taaaaccacg cttttacgta
     4321 tcttggctgg cttagaaagt tctgacagcg gagaatttta tttagacggt gtaccgttca
     4381 acccattcga gcttaaaatg aaagaacaag ttgtaggagt cgtattccaa gacttccagc
     4441 tatttccaca tctgaatatt tttgaaaaca ttaccttagc accaaaaatg gtattgaaac
     4501 agacaaaaga agaatacacc aaaaaagcag aagaattagc ggcttcatta gggattagtg
     4561 atttattgaa tatctatcct tatcagcttt ctggcggaca aaaacagcgg gtagcgattg
     4621 ccagagcatt ggctatgaat ccacgggtat tggcttatga tgaaccaaca agtgctttag
     4681 atcctgaatt acgccagcaa gtcgagcgtt tgatcctatc attgaaagaa gatggggtaa
     4741 cccaaattat tgtcacccat gatttagtat ttgcagaaaa tgtcggtgat caattactaa
     4801 aagtttcccc aattcattaa gaaaggagaa gatagagatg aaaaaaacca gttttctatt
     4861 cgctttgatc gcaggtcttc ttctttttgc aggctgttcg aacaaaaaaa cttcagcaga
     4921 taattgggcg actatcaacg aagagaaaaa aatcgttata ggtttagacg atacatttgt
     4981 tcctatggga tttagagata aagacgggaa gctcacagga tttgatattg atttagccaa
     5041 agcagtcttt gaacaatatg ggattacggt agactttcag cccattgact ggtcaatgaa
     5101 ggaatttgaa ctgaacaatg gaacgatcga tttgatatgg aacggctatt ccaaaacgcc
     5161 ggcaagagaa aagaaagtgc aatttacaaa accttatatg gaaaacgatc aagtgctgat
     5221 cacacctaaa gaatcaggaa tcacaaactt tggacaaatg aataataaga gattaggtgc
     5281 gcaaaatggg tcatctggtt atgatgtatt tacgaaacaa ccagaagtat taaaagagat
     5341 agtcaaaaac caagatgctg ttttgtatga tagttttaat gaagcattga tcgatctgaa
     5401 aagcagacgg gtcgatggtc tgctgatgga taaagtatac gcagactatt atttgaaaca
     5461 aagaaacgaa ctagaagact tcaacataac aaaaggttct tatgagagtg aggattttgc
     5521 agttggtgca cgcaagtcag atagtacatt agtgaaaaaa atcaatgaag ccctagatca
     5581 actagagaaa acaggtgaat tccaaaaaat atccgataaa tggtttggcg aagatgttac
     5641 gccgagaaat taacaaatag gggactgtga caaaagacgt gtcacagtcc ctttttccga
     5701 ataaacagtg ttctcctgcc aactccttgt gactcggagc tgaatggcag gctcacaacc
     5761 tcttggtaaa cggtgttcaa aagccagttt ttcggtatta agccaaattt atgcaaaaat
     5821 gtagaaagcc attttcacaa aatttttctt actactcgaa actaagcgac tttttcacaa
     5881 cctcctttac ataattttat tttgttgtgg aaggggtttc gtgttattct aaaagaggaa
     5941 cgattgaagg ttataggaaa ggaagagatg aatggaacac aaagaactta cctcatttcc
     6001 aaaagatttt ctgtggggct ctgcttctgc agcgtatcaa gtagaaggag cttggcaaga
     6061 agatgggaaa ggagaatcag tctgggatcg atttgtacgg atacctggaa agacatttaa
     6121 aggaacgaac ggtgatttgg cagtagacca ctatcatcgt tataaagaag atattgcttt
     6181 aatgaaagaa caaggtctga aagcttaccg cttctctgta gcttggacac ggatttttcc
     6241 taatggacga ggagaagtca atcaagctgg gctcgctttt tatgagagat tgatcgacga
     6301 attgattgaa aacgatattg aacctatgtt gactttatat cattgggatc ttccacaagc
     6361 attgcaagat gaatataatg gttgggaatc acgtcagatc attgaagatt tcactcatta
     6421 tgcagaaaca ttatttgagg cattccgcgg gaaagtccat tactggatta gtttgaatga
     6481 acaaaatatc ttcacttcat taggttattt gttagcagca catccgccag gtgtgacaga
     6541 cccgaaacga atgtatgaag ttaaccatat tgctaattta gcaaatgcat ctgtcatcaa
     6601 taaatttcac gagttagaaa tgcctggaaa aatcggacca agctttgcgt atacgccaaa
     6661 ttatccaatt gacagtaatc ctgaaaatgt tctggctgct gaaaatgcag aagacctgat
     6721 ggccaattat tggatggatg tctatatgtg gggaaaatat ccgattgctg ccatgaggtt
     6781 cttagaagaa aaaggatggg caccaacgat tgaagctggc gatgcagaat tgctagaatc
     6841 tgctaaacca gattttcttg ggatcaatta ctatcaaacg gcgacgaatg cattcaatcc
     6901 attaaacgga gttggtgctg gaaaaatgaa tacgacagga aaaaaaggtt catcagaaga
     6961 aactggagtt cctggaatgt ataaaaaagt tgagaatcca tttgtcgaac gaacaaattg
     7021 ggactgggag attgacccag aaggattaag gatcggactg cgtcgtatca caagccgtta
     7081 ccgcatgccg gtactgatta cggaaaatgg cttaggagag tatgacaagt tgacagaaga
     7141 taaacaaatc catgacgact atcgaatcaa ttacttacaa agccacgtca aagccatcaa
     7201 agaagcaatt tcggatggag cagaagtcct aggctattgc acctggtctt atacagattt
     7261 gcttagctgg ctgaacggtt atcaaaagcg atatggattt gtatatgttg atcaagacga
     7321 aacacaagaa ggatctttgg aacgatataa gaaagacagt tattactggt accaaaaact
     7381 gattgaagaa aatggagaga acctttagtt cacatactta aaacaagaag gtacggctgg
     7441 atatggccgt gccttttctt gataaaatca tttagaaatt aagaaaaact gtaaaataag
     7501 ctgaaaaatg aaagaaagcg tcagttcttt caaacaaaac tttagttccc ttactttttt
     7561 ttgtataatg aaacttgatg aaatgaggga taataaatgg cacaaaagaa acggcctact
     7621 aaaaaaagga aaaagacgaa aaaaacgaaa aagcaaaaac agcagcagga acaaatgctg
     7681 acgattggtg ttgggtttgc cttgatttta tttgctgtct tcggcttttt aaaattaggt
     7741 tttttaggaa tattgattgc aaacggcttt cggatcattg caggaaatac ttaccaaatc
     7801 ctctgtcttt tattagccgt attaggattt tggattgtca ttaagaatac agagttttca
     7861 attggtaaaa accgacgctg gtttggcggc atactctttt atttcggtat cttattattg
     7921 ttacatgccc atttatttgg caaactccat actggggaac caaatatcat ggggacgaca
     7981 tgggatttgt tagccagtga tataaaacaa agtcaagtag acaataacgt tggtggtggg
     8041 atgattggag caatccttta tcattttact tacttcttga ttgcacagcc tggaagttat
     8101 ttagcagcag tgctattact ggcaggagga gcattcctgt tcagtaatct ggaaggctat
     8161 cagctgttaa atggtatcca atcaattgga gaacgagtac aggaattatt ggaaggtgat
     8221 cctgccaagc aagctcgaaa gcaagcggca aaggaagaga gaatgaaaca gcgcgcagaa
     8281 gcgaaagaag caaggcgctt agctgcaaaa gaagctgcag aaaaagaagc tgcagaatat
     8341 gagaagaaaa aagcggtgag ccagcaaagg gaaaatactg ctgatgaatg gcaggaacca
     8401 gaaaatacag agccggagca gctaagtttt gttccaatcg acagttttca agaaaatatc
     8461 catcctgcga atctagagaa accagtacca gatacaccaa aacagaccaa tacagcagaa
     8521 ggtttttcag atgagttgcc agaggatgac ggtacctcat tagaatttga gattgaagca
     8581 gaacaggaaa atcaagacta tgaattaccg acagttgatt tgcttgattc tataccaact
     8641 gtggatcaaa gtgatgagta taaaaaaatt gaaaaaaata tcggcgtatt agaacaaact
     8701 ttccaaagtt tcggtgtcga tgccaaggtc gtcaaagcca gcttaggacc atccgtcaca
     8761 aaattcgaag tccagccggc tgtaggggta aaagtaagta aaattgtgaa cttaacagat
     8821 gatatcgcgc ttgctttagc ggcgaaagat gtccggatgg aagcaccgat tccaggtaaa
     8881 tcactgattg gtattgaagt accaaatgga aagatcagta tggtttcctt tagagaaatc
     8941 atcgaagcac aaccaaacca cccagataaa ttactagaag tacctttagg acgtgatgtt
     9001 tctggacggg tacaaacggc tgatctttcg aagatgcctc acttactcgt ggctggttcg
     9061 actggtagtg ggaagtcagt agcaattaat gggattatca caagtatatt aatgcgtgct
     9121 aagccccatg aagtaaaact gatgatgatc gatccgaaaa tggttgaatt gaatatgtac
     9181 aacggcatac ctcatctgtt gacgccggtc gtgacgaacc ctagaaaagc agcgcaagcc
     9241 ttacaaaaag tagtacaaga aatggaagaa cgatatgaaa aattcgcggc aaccggagta
     9301 agaaatattt ctggttacaa tgaatttgtt caacagaaaa acttggaaaa cggaacgaaa
     9361 catccaacct taccattcat cgtagtgatc gtagatgaat tagctgattt gatgatggta
     9421 gccagcaatg aagttgaaga tgcgattatt cgtttagcgc aaatggctcg ggcagcgggg
     9481 attcatatga tcctagctac tcaacgtcca agtgtggatg ttatcacagg aatcatcaaa
     9541 gcaaatgtac cttccagaat ggcatttgcg gtatcaagcg gtacagattc tcggacgatc
     9601 attgattcta atggggcaga aaagctctta gggcgaggag atatgctatt cttgccaatg
     9661 ggtgagaata aaccgatccg tgtgcaagga gcattcattt ctgaccacga agtagaacgg
     9721 gtcgttcaat ttgttacaga tcagcaagaa gctcattatg aagagaaaat gatgccgaca
     9781 gatgaagttg aaacgacagg tgctccagaa cagccacaag atgagctgtt tgaagaagca
     9841 aaagcacttg tcgttgaaat gcaaacggca agtatttccc ttctccaaag aagatttcgg
     9901 atcggttaca accgagcagc acgtttagta gatgaactgg aagctcatgg agttgttggc
     9961 ccgtcagaag gaagcaagcc tcggaaagta ttcattgagc aggaagaaac atcagaaacg
    10021 ttgccggacg ataatgacat gtaaaacttg tgtgatgaag ggttgaaaaa cgaataacca
    10081 ataaaaagac aaggcatgat gataccaaaa gaaaaaatgg tttttttcca tcttttctaa
    10141 tacggtatga tgtcttgtct ttttagtttt tgtgagatta aagttctatt cgatttattt
    10201 gtgaagtatt gcattaagga aatgaatcat gtatagtatg tagcgaaact ttgagaaaaa
    10261 gagggaaaat actttggaaa aacagtccat ttatggcttg acgaatgaag aattaatcaa
    10321 ttggtttata gaaaacggcg agaaaaaatt ccgagctgcc caagtttggg aatggttgta
    10381 tcaaaaacgt gtaagtaatt tcacagaaat gacgaatcta tcgaaacaat tgatcgaaaa
    10441 attatcggag cattttatca tcaatcctct aaaacagatg gtcgtccaag aagcttcgga
    10501 tggtacagta aaatatttgt ttgaactccc tgataaaaac atgattgaga ctgtactgat
    10561 gagacaagaa tatggcttgt cagtttgtgt gacgacacaa gtaggatgta atatcggctg
    10621 tacgttttgt gcaagcgggc tgctcaaaaa gaaccgtgat ctgactgctg gagaaattgt
    10681 tgcacagatc atgatggtcc aacattattt tgatgagcgc aatcttggcg aacgcgtttc
    10741 acatgtcgtt gtaatgggga tcggtgagcc atttgataat tacgacaatg taatggactt
    10801 tttgcatatc atcaatgatg ccaaaggatt ggcaattggt gctcggcaca ttacagtttc
    10861 cactagtgga ttagcacaca aaatcaagga attcgctaat aacggcttgc aagtcaatct
    10921 ggctatttcc ttgcacgcgc cgaataatga ggtgcgaaca tcgatcatgc ggatcaaccg
    10981 aagtttccca attgagaaat tgatggaagc tgtggatgag tatttagaga aaacaaaccg
    11041 tcgaatcacg tttgaatata tcatgttgaa tcaagtaaat gatagaccag agcatgcgca
    11101 acaattagca gatctattaa aagataagaa aaaattagca tatgtcaact tgattccata
    11161 taatccagtc agtgaacatg atcaatattc tcgcagtcca aaagctgatg tattgagatt
    11221 ttatgatgta ttgaagaaaa acggcgtaaa ctgtgtgata cgtaaagaac atggtacaga
    11281 tattgatgca gcttgcggac aattgcgaag caagcaaatg aaaaaaacag ttgccaaata
    11341 atcgtatcct actcgttttt tcaattacca taaaaggagc agttttctta attaaggaac
    11401 tgttcaactt aaataagaga aaagattcta tagcgaaagc tataaagctt tttttgacaa
    11461 gaaaaaccct catcaagcaa agaaatgtcc tcaatttgat gacagcctgc ttgatggggg
    11521 ttttagcaat tatttaactg attttcttat atccttgctg gctttcttgt gtagcatgat
    11581 aagtttctac gtcaagaatg gcctgtttaa atttttctcg ggtgatttca taaggcacat
    11641 gtttaatatc atttgttgcc atggctttat ctaagattgc ttctaaatcg gtcgtgacat
    11701 tgagattcat gtcggttaac gatactggaa gttggttctt tttcataaac acatagattt
    11761 tatcaaacaa atcgaactgc tgatccatca tcaataaggt cagtacacca taactgacag
    11821 atgctccgtg aaggtatccc tccatttttt ctagaacagt cgtaccgtaa tagaatgagt
    11881 gtgccatagc agaattatag ttattgttaa ctaaaacaga aaccaatccg gtagtaacaa
    11941 tgatatccga aacgacttgt tcaattgcat atgagggacg atcgtcggca ctatctttca
    12001 atgcttgcgt accataagtc aagagacgat caatgcacat ttttccgatt tggattccta
    12061 atctatccgt gtagcctaga tcatcgccac gagcagaaaa agtagattcg acttctttgg
    12121 ataaagcatc gccaatacct gcccataagt atttatcagg tgcttcggca ataattgctg
    12181 tatcaatgaa ggtgtgtaca ggtggttttg aaggaaagaa gagtccgtcc attacgcctt
    12241 cttcgttata gatgacacat accgcagtga cgggcgaaca gttcgaacca atagtgggaa
    12301 aggtgaagac tggtttagct agtttttcac caatgacttt tacagtatcg caagcacggc
    12361 caccaccgac tgcaaacagg acgtctgctt tttggacact ttcatgctgg atcaactgtt
    12421 caatatttcc ataagttgaa ttacccccgt accagagact ggcgacaacc tctatttcct
    12481 gtgtcaaagc tacttttatt ttgttttctg cctttgaaag tgcagtcttc cctccaatca
    12541 gcactacttt tgtcccatat tctttgacga ttccatttgc tttttcatac gcatctgttc
    12601 caatggtata atttggtaag aaaacacttt gactcatatt catttccttc tttcttcgtt
    12661 gaaacgggtt aaaattttaa agagtgatcg atctgactgc ggctgtagat ggagtagagg
    12721 tgaacctaag acaggataag tgatgaataa ggtggcatga acaccttgaa atcatcaata
    12781 agtatcctaa aaacgaacaa gtacaaaaac attgaaaacg acaataatga atttttgttc
    12841 ctcttagtat ttctaccgat tccctttgct tttttgtaat tatctaaaaa ttctaaaaat
    12901 taagattatt atatgaaaac aggttttatt tgtcaattca taattggttt tttttaaagc
    12961 aaaagataat ctatatcatg atgaacaaac agagtttttt cctccaattg atggctttcc
    13021 tgatctttcc tattttgaat aaagtgtttc tgtataattt ttttatttta taagtaagat
    13081 aaataatgaa agaatagaga tactgattgg tagctaaaga tttttgagcg agaacatttc
    13141 gttttttcaa aaggactgtt agaataagca agaacaaggg ggaagacgta tgataatcag
    13201 tgaatttgac cgtaataatc cagtattgaa agatcagctt tctgatttac tgagactgac
    13261 ttggccggaa gaatatggag acagctcggc agaagaagta gaagaaatga tgaatccaga
    13321 acgaatcgcg gtagcagcgg tagaccaaga tgagttagta ggatttattg gtgcaatccc
    13381 tcaatacggt atcacaggtt gggaattgca tccattagtt gtagaaagct cccgacgaaa
    13441 gaaccaaata ggtactcgat tagtcaatta cttagaaaaa gaagtagctt ccagaggagg
    13501 aatcacgatt tatttaggta cggatgattt agaccatgga acaacgttaa gtcaaacgga
    13561 cctgtatgag catacatttg ataaagtggc ttccatccag aaccttcgtg aacatccgta
    13621 tgaattctat gaaaaattag gttataaaat cgtaggtgtc ttaccaaatg caaatggctg
    13681 ggacaaaccg gatatttgga tggcaaaaac gattattcct cgaccaaatt ctcaataata
    13741 aaaagaggtt gtgacagaag taatcagctt caagaaataa gaaggaattt acgaaaatta
    13801 cttcttgaat ttttgtgaaa ttccaactta tttccgaaga agctactttt ggaacaccgt
    13861 gtaccagagg ttgtgaaaaa gtcgcttagt ttcgagtagt aagaaaaatt ttgtgaaaat
    13921 ggctttctac atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg
    13981 tttattcaga taaggga
//
LOCUS       DAYYDS010000065        13943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000065 DAYYDS010000000
VERSION     DAYYDS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.066"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACHA02_002016"
     CDS             136..309
                     /locus_tag="ACHA02_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670333.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACHA02_002017"
     CDS             949..1422
                     /locus_tag="ACHA02_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8670334.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ACHA02_002018"
     CDS             1431..1658
                     /locus_tag="ACHA02_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670335.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="ACHA02_002019"
     CDS             2294..2665
                     /locus_tag="ACHA02_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670336.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="ACHA02_002020"
     CDS             complement(2921..3163)
                     /locus_tag="ACHA02_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8670337.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="ACHA02_002021"
     CDS             complement(3195..4097)
                     /locus_tag="ACHA02_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8670338.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="ACHA02_002022"
     CDS             complement(4110..4298)
                     /locus_tag="ACHA02_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8670339.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACHA02_002023"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACHA02_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8670340.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="ACHA02_002024"
     CDS             complement(4913..5806)
                     /locus_tag="ACHA02_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8670341.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="ACHA02_002025"
     CDS             complement(5884..6807)
                     /locus_tag="ACHA02_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8670342.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="ACHA02_002026"
     CDS             complement(6856..7206)
                     /locus_tag="ACHA02_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8670343.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="ACHA02_002027"
     CDS             complement(7239..8141)
                     /locus_tag="ACHA02_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8670344.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="ACHA02_002028"
     CDS             complement(8134..8991)
                     /locus_tag="ACHA02_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8670345.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="ACHA02_002029"
     CDS             9325..10134
                     /locus_tag="ACHA02_002029"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8670346.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="ACHA02_002030"
     CDS             10174..10671
                     /locus_tag="ACHA02_002030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8670347.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="ACHA02_002031"
     tRNA            complement(11056..11129)
                     /locus_tag="ACHA02_002031"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="ACHA02_002032"
     CDS             11316..11639
                     /locus_tag="ACHA02_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670348.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="ACHA02_002033"
     CDS             11803..12057
                     /locus_tag="ACHA02_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670349.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="ACHA02_002034"
     CDS             12127..12372
                     /locus_tag="ACHA02_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8670350.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="ACHA02_002035"
     CDS             12448..12813
                     /locus_tag="ACHA02_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8670351.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12874..13437)
                     /locus_tag="ACHA02_002036"
     CDS             complement(12874..13437)
                     /locus_tag="ACHA02_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670352.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4087 a         2577 c         2304 g         4975 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
    13441 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
    13501 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
    13561 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
    13621 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
    13681 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
    13741 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
    13801 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
    13861 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
    13921 ttcagcccat tcaacgacca atg
//
LOCUS       DAYYDS010000066        13810 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000066 DAYYDS010000000
VERSION     DAYYDS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.067"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACHA02_002037"
     CDS             complement(221..1423)
                     /locus_tag="ACHA02_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8670353.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACHA02_002038"
     CDS             complement(1426..1620)
                     /locus_tag="ACHA02_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8670354.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACHA02_002039"
     CDS             complement(1667..2515)
                     /locus_tag="ACHA02_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM8670355.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACHA02_002040"
     CDS             complement(2720..3070)
                     /locus_tag="ACHA02_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670356.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACHA02_002041"
     CDS             3248..4111
                     /locus_tag="ACHA02_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8670357.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACHA02_002042"
     CDS             4735..5727
                     /locus_tag="ACHA02_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8670358.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACHA02_002043"
     CDS             5743..6627
                     /locus_tag="ACHA02_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8670359.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACHA02_002044"
     CDS             6676..8283
                     /locus_tag="ACHA02_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8670360.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACHA02_002045"
     CDS             8284..9768
                     /locus_tag="ACHA02_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8670361.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACHA02_002046"
     CDS             9945..10937
                     /locus_tag="ACHA02_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8670362.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACHA02_002047"
     CDS             10989..11858
                     /locus_tag="ACHA02_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8670363.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACHA02_002048"
     CDS             complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACHA02_002048"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8670364.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DAYYDS010000067        13730 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000067 DAYYDS010000000
VERSION     DAYYDS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.068"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1451
                     /locus_tag="ACHA02_002049"
     CDS             <1..1451
                     /locus_tag="ACHA02_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8670365.1"
                     /translation="KKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTEM
                     SNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSNA
                     ESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFST
                     PQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQ
                     AILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENY
                     EESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELI
                     KTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHA
                     VKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTG
                     NSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1800..2432
                     /locus_tag="ACHA02_002050"
     CDS             1800..2432
                     /locus_tag="ACHA02_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8670366.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3020..4459
                     /locus_tag="ACHA02_002051"
     CDS             3020..4459
                     /locus_tag="ACHA02_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8670367.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4754..4972
                     /locus_tag="ACHA02_002052"
                     /pseudo
     CDS             4754..4972
                     /locus_tag="ACHA02_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5221..5436
                     /locus_tag="ACHA02_002053"
     CDS             5221..5436
                     /locus_tag="ACHA02_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670368.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5653..5909)
                     /locus_tag="ACHA02_002054"
                     /pseudo
     CDS             complement(5653..5909)
                     /locus_tag="ACHA02_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6096..7007)
                     /locus_tag="ACHA02_002055"
     CDS             complement(6096..7007)
                     /locus_tag="ACHA02_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8670369.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7026..8030)
                     /locus_tag="ACHA02_002056"
     CDS             complement(7026..8030)
                     /locus_tag="ACHA02_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8670370.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8027..10150)
                     /locus_tag="ACHA02_002057"
     CDS             complement(8027..10150)
                     /locus_tag="ACHA02_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8670371.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10155..12602)
                     /locus_tag="ACHA02_002058"
     CDS             complement(10155..12602)
                     /locus_tag="ACHA02_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8670372.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12589..12978)
                     /locus_tag="ACHA02_002059"
     CDS             complement(12589..12978)
                     /locus_tag="ACHA02_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8670373.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13038..13541)
                     /locus_tag="ACHA02_002060"
     CDS             complement(13038..13541)
                     /locus_tag="ACHA02_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8670374.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
BASE COUNT         4220 a         2710 c         2509 g         4291 t
ORIGIN      
        1 tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc cttatagcaa
       61 cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa gaaagaaccg
      121 agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt ttgacaactt
      181 catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca tcaagtgaaa
      241 gtagtacgac ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt agtacgactt
      301 caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca agtaccgaat
      361 cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca aaaccaaacc
      421 atatagaatc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa actttttcga
      481 caccacagca aatagaatca acagaatctt tccaatcaca accaatacta tctggtaatg
      541 aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa attggtgagt
      601 ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt gcacaagcga
      661 tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac aatttatttg
      721 ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa gatttgggta
      781 atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat gaggaaagct
      841 ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat ttttatgcag
      901 gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt acaggacggt
      961 atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc tacgatttaa
     1021 ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac caagtgccta
     1081 ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt caccgaggac
     1141 ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt aaggttgtta
     1201 aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa gatggatcaa
     1261 ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat gttttgcaag
     1321 gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac cttcatttgg
     1381 aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa accttattat
     1441 tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt ggattcttag
     1501 acctttgatc aggatattga gactggaatt gatatctcga ttacgatggg tttgacaaat
     1561 aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct catattttga
     1621 gcaggtttca ctagatgaaa acaatttatc tatcttgagg atttcgtgta cgtatcgttc
     1681 agtcttgtat tgtaaattag tcataaaatc agactaagaa aaattggaaa tatttttttc
     1741 tgatttatta aaagttaatt gaaattatat ggtacttctt tcttgggagg gtaggtgcag
     1801 tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat ggatataaaa
     1861 aatatcgttg cctatgtgac tgtgggaatg aaacgattgt atatcgatca aacttactat
     1921 caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa tataaaaatt
     1981 tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg cgaagtgaag
     2041 gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta tcggcaaagc
     2101 gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag catcacattg
     2161 aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac tgttcatgga
     2221 aaacaaaatg ggtttgtcat tgtgagtgtg gcagtatgtg tgagataagt tatagtaact
     2281 tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca aggacatgtg
     2341 ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat acaagtggtg
     2401 ttaagggagt ttatcgttct aaagagaaat agattgcgta tataacttta aaaaaaaaga
     2461 ggtactattt aggatcgtat gattctatta agagtgcagc tcaagctaga aaagatgctg
     2521 aaaataaatt gtttcaacct ttgattgaca aatatcgagg taatcattaa ttattgtgga
     2581 taataattaa cagtgatata taaacatcta taatatatta ttcatactat tgatatttag
     2641 tataatccta tggaattgaa tatcatagca atgtatttga aaagcaaatt ggaaaacaaa
     2701 taggtgcaac accataaata tgaaaaaatt gggaaaaagt agaatggaaa tttgcttacg
     2761 tctcacgaga tagctcacac tattaatctg aagaacaatg aagtcgactt ttagaaattg
     2821 ctagaagaaa gtaaagcgaa ttatcactag ttactataga gtaagttcaa ataaagcatc
     2881 tccaacttac atggttacat gaggatatca aatcgaaatt actcaagctt cctatggcta
     2941 gagtcgcgag aagttgttgg gcagtagaca taggttatat tgttcttagc tacacgatca
     3001 ttagaaagga aatcctatta tgtatagtta tcaagaagat gcaaaagtcc aaaaaaatcg
     3061 ctggtggatt ttgattgcta tatctatgtt taccttcatg tccacattgg atggcagtat
     3121 tgtcaatatt gcactaccta ccatctcaga tgatatgggc ataccaatga atcaagcgga
     3181 atgggtagtg tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt ttggtaaaat
     3241 tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt acgctgatct ttgtcttggg
     3301 atcgctgttg tgtgggttca atcattcgct aggtatttta ttgttcgcac gggtggtaca
     3361 agccattggt gctagtatga ctatggctac aaatgccgga atcatcacgg aagtctttcc
     3421 catacatgaa cgtggtcggg cattgggagc aataggtgcg tttgtttcat taggctcgat
     3481 tgctggacca ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt acattttttg
     3541 gatcaatgta ccggtgggaa tcgtaacgat gttgattgct gaaaaattct taccaaaaga
     3601 tatcaccatg agcggccata caatcgatgg agggggcttt gctgccattg cggctacgat
     3661 tatcagcttt tttggagcaa tctttttggg gcaagaaagt ggctttttcc atccagtccc
     3721 ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag ttgaaaagaa
     3781 agcagcctct ccattgattt ctttcggtat ttttaagaac aagattttta ccatgagctt
     3841 gattacagcc ttgttgattt tttcttccaa cttctttgtc aatgtagtga ttccttttta
     3901 tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg ctgttgatga tggtctttcc
     3961 tttgatgatg gtcgtcgggg cgcctttaag cggctacctg accgaccggg ttggcccgga
     4021 gattttggta ttagtaggct tgggcttatt gtcggtaacc caggtcatgt atatgttttt
     4081 gtcagtggac tctcctttgt ggctatacat tgtcgctact gccatcatgg gtgccggcaa
     4141 tgccttgttc cagtccccca ataatacgat ggttatgagc tcggtcacta tcgaaaactt
     4201 gggactggca ggctcgatga attcctttgc ccgaaatttg gggatggtca gcgggatttc
     4261 gctttctacg acggtcttat atcaagcgat gtctgctaag atgggccagc aagtaactaa
     4321 ctatatcgca aatcggccgg atgtcttttt atatggaatg aagattacct ttttaggcag
     4381 ctttgttttg tgttttgttg cctttttcct gactatctgg cgtatcaaaa aacaaggagg
     4441 tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc tgcgtatggg
     4501 tgagtgggat ttccatggat gatggaagat gaagtgaact tctaaaagcc gaaaaactca
     4561 agtattatct tcaaaatact agtgctcaat gagatgcttg atttgatcac aagaagactg
     4621 cgaaagccgc tggcaaccgc aagctaggta tcccaccgat ggactacgtt ttagaggcta
     4681 ctggttatga atacggagcc aacgctctca tctgcataag aatacatcac ccagattatg
     4741 taaagatttt cgatacttct gtcaaaaaat cattaagata gataaatgat ttccgtctag
     4801 tcaaattggt tcagaatgta gacacacaga tggtaagaag tcactcacaa ttcgagaatg
     4861 gacttgtcct atttgccgta tacatcataa tcgaaacgtc aacgatagta tcaatatttt
     4921 gaccaagggc tatgaataca agcattgcct tagaaaacat caagaacctt aggaacaacg
     4981 gggatagctt ggttaataag agataccgct gtttaggctt gcaagtatac tcttttatca
     5041 agaattccgt ggctttagtc atgagaggct taatactatg aaaattttct ttagcatttt
     5101 tgatttaggt tccttttcta attataataa gatgaccact acagtaacct actgaaatat
     5161 ataaacagcc actgtagtac cattttcaaa gtagtctatg agaggagaat cttcattctt
     5221 atgaacgctg gaattgcgga tatgaattta ataaaaaaaa cccttaatga ttttacttcc
     5281 aattcaatat caaaaggtac tggtatcaat ttaagcacaa taaaaaagct gaaaagtggt
     5341 gaaagatcag tggaaaagct gaatctttta gatgctataa aaataacgga atttgctatg
     5401 aaaaatggaa aagctgagat tgaaatatgg agataattat cgtctagtga attacttgcc
     5461 acttagctga atttaacgat ccttggtgct tgaaggggga gattcttggg aatagagcat
     5521 acttgcaagc ctatgtcttt actaacaacg atatctctta ttaaccaagc tatccccgta
     5581 gttctaaggc ttttactttt atctaagcaa taatcgtatt caaaggaaat ttttgaccct
     5641 tagaaagtct cattttatca gacgtgagct taggcattgc tgaattttgt taattcggtt
     5701 ttaggttgtg ctttgagcat aatatgaaag tattctttat cacgatttca ttcatctaag
     5761 gtaatgtgat gtgattctga aattctttca aaggcagttt ttgcataagc tgacattaca
     5821 tcatcaatga catgtttaca atgtttcact actaaaataa gggggtaacc aagaagaaaa
     5881 actgaatgat tatttgtatc taattccatg gtatatcaac catttcctat atagactgat
     5941 tatgacatgg attaaaataa aaaggcacta tgtcttaatt gtcggttttc gagttaccga
     6001 atggcattca tcacccacct atagaggatg ggcgacttct gcctgaaata agttacacta
     6061 cttataactt ctaataaaat tagattgagt cattattatt taacatcaac gattttccag
     6121 tttccatttt tttgtaatac taatttgtac tgaaagtagc tagtggtttt tgatacatta
     6181 tcaaggtatt ttatctttgt actcactaga aaattttttc cgttttgtag ataaactgga
     6241 ttctctaact ccgaaaactt taagtcaata ttgattggtc gcatcgcacc attttcaaca
     6301 taatactcca attctttttc agaagctgat ggataaagtt taaaaaatgt ttctaaaaaa
     6361 tcggtgattt cttccgttgt tttggcatca atttctgaat cgttttctac ttgttttgct
     6421 tcatattttg ccttgtctgg ctttccaatc attgttggca aactagttac taaatgattc
     6481 ctgttttcat cttcaaagat tgtcactcga tatgcagaag aaattgattg cgtctttttt
     6541 ccctctgtaa tatgttgctc cactaaaaat gaaacgacaa attcttccga gtgtttttca
     6601 acatctagaa tttttacaga ttgaacttcg gaactcgtgg gaatatctgc tcgaaccata
     6661 tcttggatta aagcgagacc gttttctgta aggtactgtt cgagattact cattctcttt
     6721 tcaattgctt ctttgttatt tttccatgaa aaatattctt tcgcaaaatc ctctgtaaaa
     6781 ctttcaacgc cagaagtatt cactaccttc tcttttataa ccactttttc atgagttgta
     6841 tgttgatcaa tggcggtgaa atttttataa acgccaaaag caagactaca agacaataaa
     6901 agccaaaggg caagcaccat ttttcgatgc ttacccacgg atacaactcg ttcttttttc
     6961 ttttttgggc tcttttcctt ttgattccgt tcaattttta tcttcattct ttttcctctt
     7021 ttctcttatt tgtatcggcc agctccgata agatgctgcc gccaatagtt ttccgttaga
     7081 ttcgtccacc ccactggatc tcctgcatga aacatgcgat tctcccctgc gtaaatgccc
     7141 acatgggtca cataagtacc ggcatcatag gttgaatgaa agaaaactaa atcacctggt
     7201 ttggcatctt ttagatcaat atgagtcatg gcatcatatt gatcctgagc cacccgagga
     7261 agtttcaggt caactttggc ataacaccat tgggtcaagc cactacaatc aaaccccgtt
     7321 tctggacttg agccaccaaa cacataggcc gttccttcat acttaaatgc ctcatcaaaa
     7381 atttcttgga ctattttgtc atcaaactga gttgtagtta ggtattgctt cactaataaa
     7441 cagtaaaaca tattgccata attataccgc cacccaccat tttccttaat cgcgattggg
     7501 ttggggtaat cgactttttg accacctgat ttttcctttg aaaaagattc agctaaagca
     7561 aaactatgct tcttcccgtt tttcccgaca tgatctagat acccgccacc atagttatag
     7621 gcctgaataa tactatcttg atcacaccct agttctttgg aacgcttaac caaagacgca
     7681 aaatagagac agccttgttt gatggatgcc tcggtactta atgaattagg tggtaatccg
     7741 gctgactcag aactttgcat aacgtcttcc attgtgccac cagattccac ttgaataatc
     7801 gccaaaagaa ttggaatata ctctgttata tccgattctt tggcatactt ttccaccatt
     7861 gcacggtgtt tcaatacttc ttctgagaca ctagttccgc caggagaagt gccagaacta
     7921 ccatttccct cactgtcttc accaaacaac aaacccacac acatcaaaat acccataaaa
     7981 gagatggtca gaaaagttaa tgaaataaca cccactttct tcaagttcat tttttctcac
     8041 ctgaccttga ttttggacga tggttcattc ttttttgtac tttttgattg gtctgctttg
     8101 gcagagtgga cctctgtttc acttgttgga aagaattttt cttacttgct gattggcttt
     8161 tcccttttga aatgatttct tgactagtta gttttcgttt gatttcggga ttcagatttg
     8221 ctctcacaat ccgctttggt gcttcttttt tcagcatcat atctttctga ggttgagctg
     8281 ctttatctat tggaatattc gcccttcttt ttccctgacg atttgttgct tcactcagta
     8341 tcttgcgttt ttcgtccatt ttcgcatttc ttcgttgtcg ttgtttttct cgcaaatcca
     8401 ctcgatccgc tttttcttgg aacagtccgc gcttgaattc ttctggtgct tttttcgact
     8461 tttcgattcc tttatggaga ttgtatttca aattcgttgg cgtattcctt acctgatcct
     8521 ttacttgttt agtcttatct ttcaccaaag cttgggtatc caaaactttg ccgatttttc
     8581 tcccagccaa attcattcgg gattgtttct tattatttga aactggattc tccttagtta
     8641 agtcagatag tatttcgtga tcgttcggta atcgtgaatt ctttcgtaga gaagaaccgg
     8701 atggcttaaa tttggatttt gattgttcct ttgccaactt atttccgact gctgcaccac
     8761 caccaaaagc caaagctcgt cccacatttc gattgagttg ccgcaatttt cgattcatta
     8821 acatttgcgg ttttcgcatg acgcgtcttc ccattgactg gctatcatta ctctggaggt
     8881 tgaacatact cataatgtcg cccaatttga aatagatacc ggcaaaggtc acaatttgca
     8941 gaaaagcaac catgaaaaat ggataggttg ccgaaatatt gaaaaacatc gtggagatgc
     9001 taaatgcagt cgtaatcacc aaagtaacac ccgctcgcat cataatcgta ttgaataaac
     9061 gaataatcgc cttctttcct aaactttcat aagtcggcaa catggacaat aagaaactaa
     9121 ttggcagaaa catcgcaaag ataatgaaga gaatttgcga gaacaacata atccctgtta
     9181 gaagaaaaac aaagaaggaa atgccaatat tgaacaatac caaaaagacc acggtcccta
     9241 aacggttcat ggtcttggta atactgagat tcgcattgtc attgtcttca atctctgcct
     9301 tgaccgcttc ttcccgatct ttgcctttgt tttcatctgg actcacagat agaatcttgt
     9361 tgacccggtc ttcaccaatt tcttctatgt tggaatcatc aaattgcagt agtaaccaag
     9421 gttgttggac ttgaatcgaa aacaaactat cccgaatcaa atccacacta tcttttcctt
     9481 ggctttctga attgggtaca acgatctcgg ttccaagggt aagggccgct tcactaacat
     9541 ctgagctaaa gtcattgatt tttgtgatat aggtaggcgc ataagcaatg aacgaacccg
     9601 atagtagaaa aatcatcaca aaattaatca cggctcgaac tgctttcgtg gtttctcgtt
     9661 ttaataatcc ggtatacgcc acatacaccc ccagagctaa aatcatcaat aatagaaacc
     9721 caaaataaaa tccggaggtc tggagtccat tttcagtaat accagccaat gtttggatat
     9781 tctttccaat actttcagcc gtatccgaaa tgaaatctaa tttgtaagcc tcttgaacca
     9841 cataacccgt ggcgttcgac aaatacaaac tcaccgtcca aatgaaatta gtaatggcat
     9901 aaagaccata catcacgctt ttccctaagc catcacccca attccaaggc aaccaatccc
     9961 aagagctatc cacaaagaaa tctagttgat agttgttcag tgaatatttg gaatatagat
    10021 ttcctgcttc aactgtatca tcaactagcc ctgtcgcctc ggctaccgta ccaactaaac
    10081 tcaatagaac tatcagagtg agtatgctaa taaaaacgat tcccacaatc cgaaaaagtt
    10141 tctttttcat cccctcactc cattctctct tgaacaggtg gacgggtatc aaaggcatgg
    10201 aagagttctt caaagactgg atgtacctga accacgccta cccgaccata caaatcctgc
    10261 atcaagcatt ggccattttc caaatctcgt aggcgttttt gattgctttc atcttctcga
    10321 tccaagccaa agaattccaa ggttttcttg atttcatgaa tatcggtact gcgaaacgca
    10381 aacttcaacc cgatattatt cttcaagcgt tcatccagta aatcatcact attttgcgtg
    10441 acaaagtaaa ccccggcatt catagcccgc ccagcacgaa cgagcttatt cgataatgtt
    10501 ttcccttgcg ctacttgaag aaaactccac gcctcatcta agtctacaat tttgaagatt
    10561 tctcgattac tatggataaa gtccaaggca aaagtggaaa tgacgattag catggaaaca
    10621 ctaagcaatt ccatggtggt atattcttca aaagtggttt ctgcatctgg taaaaccaaa
    10681 tccgctactt gaatgatatt taactgccta tctaaactaa tcgactgggt aatcgaacca
    10741 tctgagaaaa gcaaatgggc aaaatcataa tcggtaaagg actcgatgtg atccgcgata
    10801 ttttccgcaa ctggtgagcc ttcttttcga agctcattaa ttacttgtaa taatccccgt
    10861 tgattgcttt gggtaaccgt ccgaatggcc ttacgcaaca ctggaaactt ctccccatct
    10921 cgactagaaa ttcctgttaa aaaggtcaga atatcaattg ctagactttc agaatccttc
    10981 tttttctcca taatcacata aggatcaagt aatccttgat tttttggctt actagttaag
    11041 ttgagaatat tgatttcatc cgcaatttct ggcaaggttt ctttccagcc accccgttcc
    11101 ccttttggat cgacaatcac agcttgtcca ccaaaaagga cggcgtaata aactaagaga
    11161 ttgttactaa aggactttcc gccaccgagt gaaccgagga atgccgctgc tagcgcattg
    11221 gtaactgaat ccttgacccc ttgtgaggcc aatgccggtt tcaggtaaac attgcgcccc
    11281 gtatctacgt tataaccaaa gtaaatgcct tccaactccc ctaacatctg agtggcgcca
    11341 aaaccaagtc cggcgagaaa atcagacgta acatactgga tataatcatt catgtatcgc
    11401 ttactagaag gtaagaattc ttcatgtagc cccatcatat cgccaaaagg ccggactagt
    11461 tttacattgg tatcatcata aaaatctagc acttcatcac accggcgctt tagttcatct
    11521 actgattcgg cacttactcg aaccacatag ctcagtttgt acatggattc tttggactga
    11581 tccaaggtag tttccaattc atctacggaa tccaatgcat ctaagacatt ggaatttgtt
    11641 tcattgtcag attgataggc atggttgtcc aagtctttta attccttttt cttattgcga
    11701 acggtagcaa gtgccttttt gttcgtcaca atctccacat tcatactagt gtcaattggg
    11761 aatgtaaatt gttgttgctg gtaatagaaa agttctgagg agggaaactc catttccccc
    11821 acaattgtat taattgttaa gtacgctaca tatgattcat gattctcatg ttccatgcat
    11881 aagtaccggg gcttttcttc aatcaaacaa cgactgggac gtaataagtc gtaacgtttc
    11941 accaaagttt ccgacttcag ctttttcttt ggtagctgaa attcatattc ttcaaatggt
    12001 gtaccctttt cgccatagat atgttcaatc aaatacatca gatcactcgg tgtgactcgt
    12061 ctgactttaa atcttcgagc caatttactt tccattagtt tttcaagctt ggtatatcgg
    12121 cgaatttctt cattggataa ggaaacaaaa tcgcccatca aatgatgatt cactccatag
    12181 acaaattctt gaaagccgga gaagaaagat ttcttgagat tctttagatt gacctcttca
    12241 tctgtcgcaa tcagtttgaa gccaataaaa aagcgataat caatctgact atcgccaatc
    12301 atggatacta atgcctctgt ctgtaaatca attcgttgtt tcgctacttc tgttaatcgc
    12361 ccggtaattt ccttttttga tcgttcttgc gtggcacgaa tactactttc tgtggcaatt
    12421 tgtaaggcat gaatcttgcc ttcacgattt tgcgcaatca attgacgaaa gttatcgtgg
    12481 acttgatatt tctgttcagg tgacaaaaag gagtaattgt aaggtattaa ctcatagtag
    12541 gcaaaacatt ctccctcttg attaaagaca agattatttt caatatactt aatcgggtac
    12601 atagttcaca ctccttacca gagtaatggg ttcgtcccat cgtttcttct gttctttgac
    12661 tttctttcct gcgaaagtga ccttaaatcg taagtgatag ctaataaaag atcgtaaaaa
    12721 tcccaatggt ttcttaccgt caaacgtttt ttgcgacatg aaccaagtaa ctgccaccgg
    12781 tatacctaag tattttaaaa aggccccctc tatcatagat agtggaggca catgggcaaa
    12841 tacgatgaca aacagcaagg ataaaacaaa ccaagccatc tggttaaaag tcacaggaaa
    12901 aggcagttga aagtcattaa ttgcataaat gaccttttca actgcccaaa tactggtgta
    12961 gctttttatt ttcttcatca aatcattcct ttaaaaagaa ggatagttta gaaatcaact
    13021 acccttcaat aattttgtta atatggatac tcaaaaacac ctcgactcgt taccaaataa
    13081 ttgcctccaa tctccaagtc tctaccacac gcatcaaaat caatatacgt cttcaagtgt
    13141 tctggtactt ctccaaaatc accagcttct acaatcaaga aatatgctac atcactcata
    13201 tcatcgcaat ctggataata gataatatca tccacatgct ctaccatctc ctcaaaagaa
    13261 ttgaagaaag catgttgaat ctcagaagca acttcaccaa ctggcgttcc ttcaagttcc
    13321 tccgctaagc cacacaaccg attaatttcc tcaattggcg tatactcatc aatctcaaag
    13381 ggaagttcat aatcatgaat tgcatattcc tcgtattcgt catttaaccc aattcgttct
    13441 ttcacttcat caaaatcgac aggtggggta aaccacgccc ccaccaattc accttcgtta
    13501 tatttcccga ggttcgcaat ataaacacgc atctgttcca tcgtgcttcc cccttacgca
    13561 ccgacaattc ggttaaacaa actgagtaag acatccttca ccccagaagc attaaagacc
    13621 aatccgaccg caatcaaagc aatcaccaaa aagccaatca gtttggaaaa ctcccgctta
    13681 aatcctaaaa acaaaccaat gaccacaatt gccagtaaga ctaaggattg
//
LOCUS       DAYYDS010000068        13711 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000068 DAYYDS010000000
VERSION     DAYYDS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.069"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..477
                     /locus_tag="ACHA02_002061"
     CDS             139..477
                     /locus_tag="ACHA02_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670375.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="ACHA02_002062"
     CDS             complement(682..1257)
                     /locus_tag="ACHA02_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFM8670376.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="ACHA02_002063"
     CDS             complement(1989..2570)
                     /locus_tag="ACHA02_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8670377.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="ACHA02_002064"
     CDS             complement(2753..3379)
                     /locus_tag="ACHA02_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8670378.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="ACHA02_002065"
     CDS             complement(3401..4732)
                     /locus_tag="ACHA02_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8670379.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="ACHA02_002066"
     CDS             complement(4887..5288)
                     /locus_tag="ACHA02_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8670380.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="ACHA02_002067"
     CDS             complement(5374..5580)
                     /locus_tag="ACHA02_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670381.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="ACHA02_002068"
     CDS             complement(5675..7177)
                     /locus_tag="ACHA02_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8670382.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="ACHA02_002069"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="ACHA02_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8670383.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="ACHA02_002070"
     CDS             complement(7748..8365)
                     /locus_tag="ACHA02_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8670384.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="ACHA02_002071"
     CDS             8586..9959
                     /locus_tag="ACHA02_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8670385.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="ACHA02_002072"
     CDS             10034..11146
                     /locus_tag="ACHA02_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8670386.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="ACHA02_002073"
     CDS             complement(11218..12855)
                     /locus_tag="ACHA02_002073"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8670387.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..13631)
                     /locus_tag="ACHA02_002074"
     CDS             complement(13023..13631)
                     /locus_tag="ACHA02_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8670388.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3700 a         2886 c         2456 g         4669 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    13621 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    13681 ttatcattta gaaatatttc ataggtaaaa g
//
LOCUS       DAYYDS010000069        13690 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000069 DAYYDS010000000
VERSION     DAYYDS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.070"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..344)
                     /locus_tag="ACHA02_002075"
     CDS             complement(<1..344)
                     /locus_tag="ACHA02_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8670389.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTG"
     gene            complement(640..1512)
                     /gene="scrK"
                     /locus_tag="ACHA02_002076"
     CDS             complement(640..1512)
                     /gene="scrK"
                     /locus_tag="ACHA02_002076"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8670390.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(1776..3722)
                     /locus_tag="ACHA02_002077"
     CDS             complement(1776..3722)
                     /locus_tag="ACHA02_002077"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8670391.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            3907..5346
                     /locus_tag="ACHA02_002078"
     CDS             3907..5346
                     /locus_tag="ACHA02_002078"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8670392.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            5348..6310
                     /locus_tag="ACHA02_002079"
     CDS             5348..6310
                     /locus_tag="ACHA02_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8670393.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(6480..7906)
                     /locus_tag="ACHA02_002080"
                     /pseudo
     CDS             complement(6480..7906)
                     /locus_tag="ACHA02_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            8149..8604
                     /gene="tnpA"
                     /locus_tag="ACHA02_002081"
     CDS             8149..8604
                     /gene="tnpA"
                     /locus_tag="ACHA02_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8670394.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            8869..8967
                     /locus_tag="ACHA02_002082"
                     /pseudo
     CDS             8869..8967
                     /locus_tag="ACHA02_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(9190..9420)
                     /locus_tag="ACHA02_002083"
     CDS             complement(9190..9420)
                     /locus_tag="ACHA02_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8670395.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(9650..10468)
                     /locus_tag="ACHA02_002084"
     CDS             complement(9650..10468)
                     /locus_tag="ACHA02_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8670396.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            10629..11318
                     /locus_tag="ACHA02_002085"
     CDS             10629..11318
                     /locus_tag="ACHA02_002085"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8670397.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            11332..12834
                     /locus_tag="ACHA02_002086"
     CDS             11332..12834
                     /locus_tag="ACHA02_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8670398.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            12847..13323
                     /locus_tag="ACHA02_002087"
     CDS             12847..13323
                     /locus_tag="ACHA02_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8670399.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(13420..13530)
                     /locus_tag="ACHA02_002088"
                     /pseudo
     CDS             complement(13420..13530)
                     /locus_tag="ACHA02_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4458 a         2294 c         2435 g         4503 t
ORIGIN      
        1 cccgtcttaa accggcgata gagggtactt actgaacaag agaaggttcg ctctgcacgg
       61 ccaataatga cgtctggggt ccaaccttca gttgatcttt ttgcaatgta ttctttttcc
      121 tcagcaggaa aaatgatttc ggttctccca caacgtcgct tattttcttt gtattgttca
      181 aagcattcaa gtgctgttcc accacatttg agaaagttat agacattgta aattgtttgg
      241 cgtccacgtt taagctgctt cgcaacgata gcaaccggag tttcttgatg aaaatatgct
      301 tctatcatta caagctcgtt tggtgtaaga tgggtataag tcatttatgt tcactctcct
      361 tgtatgcttt agcgggtatt acgatttgag tgtaacataa atggcttttt tatttgtctc
      421 gcttaattat acaaacggcg tgtaaaaaac tattgcacaa tacaagtggc ctacggctat
      481 cgaaatttcc ataactttcg agcaagaatt tatctcattc aaggcttgat cttccaagta
      541 aaacaaaaac cagttaagca ctctgcctaa ctggttatga ctaaattttc agttgtatct
      601 ttctctacca acactaaatg tcgaagagcc ttttttcgtt tatgaatgta agaccgcttc
      661 tcgagccaat gccaaacaac cgagcgttcc agcgttgtct tctaatgctg gggtcacgat
      721 atatttgctt aactcaggtg tttcgacata cccattgacc aattttgcaa atgcgtcgtg
      781 gacgttttgc agcatatggc gttgtttcat cacaccaccg ccaaaaatga tcacatctgg
      841 agaaaacatc aaggttgtat tgtaggcaca ttgagcaata taggaggctt cgatttccca
      901 ataaggatca tcttctgcaa cctcttgtcc ttttttccct aagcgttttt cgatggcggg
      961 tccagcggcc atcccttcta aacagttgcc atgaaatgga cagctgccag caaagtcatc
     1021 ttttggatga gggacgacga gcatatgccc catttctgga tgactaaacc cttcgataaa
     1081 gcgcccttct tgtaaggcac cgccgccaac acccgtaccg atcgtatagt aaacgacact
     1141 gctcaatcct ttgccactgc cgaagacata ctcaccatag gcagccgcat tgacatctgt
     1201 tgtccaagaa atcgggatcg gaaattcttt tttcatggta ccgatgaaat caaaattttg
     1261 ccatgccaat ttcggggtcg aggtaatgta cccataagtg gcggaatcgc gatggatatc
     1321 gataggccca aatgagccaa tcccaatccc tgctaaatct gcttggtatt gtttgaagaa
     1381 gtcaatgact aaaggcattg tttcctcagg tgtcgttgtc ggaaagctca ctctttcctt
     1441 gatcgtcatt tcttcatcgc caatggcaca cacaaatttt gtgccgcctg cttcaattgc
     1501 accgtatagc atagacattt ctccctttat tttagaatga aatcactaaa taaatgacta
     1561 tatatcatat ttaaattatt tttttattta gtaacatgac aagtaaataa ctactatttt
     1621 ttctttttga atttttatca ttttttaaaa ttctaataat ttttcataca attgatatat
     1681 gaaaagatta aataaaatgt tattctcaaa tatttgatta atctatatta tttgattttg
     1741 gaaagaaaat tccatcttta ctcagggaaa tatatttatt tgatttcaag taaatcagta
     1801 ccctcagtga ctgtattttc agctactata tttacttctg aataatccga agtattggtc
     1861 acaataatca ttgttgtatc atcaagacct tctttctcaa tcattgaacg gtcaaatgct
     1921 ccaagaatat cgccttttct aactttttga ttagtagtaa ctttttgtgt aaatcctttt
     1981 cctcccattg atacagtatc aataccaata tgaattagaa cttcagcacc attatttgat
     2041 ttaataccat aagcatgtcc agtttcaaac gtgagttgaa tagtaccatc tactggagaa
     2101 tggaccgtgt ttccatttgg tttaatggca atcccttttc ccatagcttc tgatgaaaag
     2161 actggatcat ttactgaaga aagactaatc actttaccag aaatgggact aacaattgtt
     2221 tctgatgaaa cttttgtttg tgttgattca acatcagtag atattggatc tgttgttgct
     2281 acttttgaag aattaactgg tgtttgttca gcaatttctt ctgctttaaa gataccagca
     2341 gcttttgttt taccgtagaa gtaagtaatt gcaaaagcaa ctacaaagct aatcaattca
     2401 cacaatacgt aaaatggaat agatgttgca ttaattgaaa gaaatcccaa gaaacctgct
     2461 gaaccaagtg aaacagcaac tactttaagt aatcctgcta ctgctgtagc aattcctgaa
     2521 ccaattaaag cacaaaagaa tggaaagcgg tattttaagt taactccgaa caaggcaggc
     2581 tctgtaattc caagaagtgc tgagacaccc gcagaggaag caagactttt tgtcttagaa
     2641 tttttagtta agaaccaaat ggcaaatgtt gcagcacctt gagcaacgtt tgccatagat
     2701 gcaactacaa agataaagtc tccatgtccg ataccatttg tccacgctga aagtaattga
     2761 gtttcaatag ctggaaatga ttgatgtaaa ccagtcatga ctataagtga atatgttcct
     2821 ccaaaaatac ccattccaat aaatccagta gtgttatata gccaaacaat aatgtctgta
     2881 attccattag acaacaataa cattacaggg ccaataactg tgaaagtcaa aaatccagta
     2941 atcattactg ataataatgg cgtgaatgta aagtcaatag atgatggcaa ttttttatga
     3001 aagaattttt ctaatataga taatatccaa acagctgcta aaactggaat aacttgataa
     3061 gcatagtttg tttgtgaaac atgataacca aaaatattcc aatattcagt tgagccacca
     3121 agatctggtg ttgtcatgat cataccaatt gcagcaccaa gaaattggtt tgctccaaaa
     3181 cgtttagcag ctgaaatacc tactaaaatt ggtaagaaca tgaatggtgc agcggacatc
     3241 agttggatca ttgaggaaat accttcaatc gcaggataca tttcttcaat tgattttggt
     3301 ccaaaaagtc cagcagctgt taaaaagttt tgtaatgcca ttaataaacc acctgcaaca
     3361 agggcaggta taattggaac aaaaatatct gataataatt taattaatgc cataatgggg
     3421 ttgaattttt tatctttact agcaatttgt tttaaatcat ctgttgaaat ttccgaaagt
     3481 ccagtttttt tgataagttc gtcataaacg aaattaacat cacctgcacc gataatcact
     3541 tgatattgtc cattaatttt aaatgtgctt ttcacatctg gattttcgtc taaagctttt
     3601 tgattcactt tcgtgtcatc ttttagtacg agacgaagac gtgtagcgca gtgtgcagct
     3661 gcaataaggt tatcttttcc gaccgcttgt attacgtcgg aagctacttt actgtaattc
     3721 attcgaatca ctcctttttt atttttacgt gaaaacggtt cactgaaatg caaatttatt
     3781 ttagcatatc ttaaaaatat gtcaaacgtt tgtcatatag tttatttttt taaatgaaat
     3841 gattgatttt tcaacggaaa acgtttacca tactagttgt aaaaagataa attgaaggag
     3901 taaaatatga acttacctcg agaaatacga tatcgtgctt attctgattg gactaaagat
     3961 gagatggata aaattaagga taatgtaaaa cgatcacctt ggcgtgctag ctatcatatc
     4021 gaacctaaaa caggattgtt aaacgatcct aatggttttt ctttttttaa cggaaaatat
     4081 acgctttttt atcaaaattg gccatttgga gctgctcatg ggttgaaaga atgggtacac
     4141 aatgaatcag atgatttagt tcattttcat gtaacaggag cagaacttct tccagacaca
     4201 aaacgagata gccacggcgc ttattccggt tctgcatatg aaatagaggg gaatctattc
     4261 ttactctata caggaaacgt tcgaaatgaa aattgggaac gtgatacact tcaaatcgga
     4321 gcatggatgg ataaagacta caacattacg aaaggtgaga atgttcttat tcataaacct
     4381 gctgatgtaa ctgatcattt tcgtgatcca caaatgttta attatgaagg acaattgtat
     4441 gcgattgttg gagcacaaaa tttagaaaaa tcaggcttta tcaaacttta caaagcggat
     4501 gacaataacg tggaaacatg ggaggaagtt ggcaaccttg attttggcgg cactggttca
     4561 gaatacatga ttgagtgtcc aaatctagta tttgtagaca aaaaacctgt tctcatttat
     4621 tgtcctcaag ggttggataa atctgagcta aattatggga atatctatcc gaatactttt
     4681 aagatctgtc aatcgcttga catagaaaaa gtaaaacttg tgggcgcttc agaaattcaa
     4741 aatcttgatt atggatttga agcgtatgcg actcaaggat tcaatactcc tgatggtcgt
     4801 gctctgattg tcagttggat tggactacct gatatcgatt atccaactga taagtatgat
     4861 tatcagggag caatgagtct tgtaaaagaa ctttcaataa aagatggtaa actttaccaa
     4921 tatccagtag aagctattac atcattacgt gctagttctg aacaatttac agcaaaagca
     4981 gaaacaaata atacttatga gcttgaacta acttttcctc caaatcagaa atcggagtta
     5041 cttcttttct ctgatagtaa aggaaatgga cttagcttga ttgtagatac caaagaagga
     5101 aaaattattt tagatcgcag caaagcaggc attcaatatg ccactgactt tggtacgatt
     5161 cgtgaatgct caattgatac taaagaaaca actgctaaca tttttgtaga taactcaatc
     5221 attgagattt ttattaataa aggagaaaaa gtgtttacta gtcgtgtatt ccctgaaaat
     5281 caccaaaatg gtattcaaat caaatcaggc gaacctactg gaaaatactt tgaattaaaa
     5341 tattaaaatg gtagcaaaat taactgatgt cgctgaatta gctggagtta gcccaacaac
     5401 cgtttcacgc gtcattaata ataagggata tttatcagaa aaaacaaaac aaaaagttca
     5461 tgaagctatg aaaactcttg gttataaacc aaataattta gcacgtggac ttcaaggaaa
     5521 gtcacctcag cttattggtc ttatttttcc taatattagt aatatctttt actctgaact
     5581 cattgagtat ctcgaaatag agttatttaa acatggatat aaaacgatta tttgcaatag
     5641 tcaaaatgat cctgctaaag agagagaata cattgaaatg cttgaagcca accaagttga
     5701 tggcataatc tcatcaagcc ataatcttgg aattgctgat tatgagcgtg tgagagcacc
     5761 tattgttgct tttgatagaa atttagctcc taatgtttcc attgtttctt cagataactt
     5821 tgaaggggga aaacttgcag ctaagacttt acaaaaaaat ggttgtcaaa ataccattat
     5881 gattacagga aatgataaca cggattctcc cacagaatta cgtgcactag gtttttcttt
     5941 tcaaaatcca aatggaaaaa ttttcaaaat tcctaataat ctgtctacta tccgtcgtga
     6001 aatggaaata aagtcaacta ttataaatca caaaccagat ggtatttttg tttctgatga
     6061 tttaacagcc attctcacta tgaaaattgc tcatcaactt aaacttaata tcccagaaga
     6121 tttaaaaatt attggatacg atggtacctc ttttattgag aaatttttcc cacaattgac
     6181 aacaataaga caaccaattg atgaaattgc aagtcttatt gttgatattc ttctgaaaaa
     6241 aattaaaggc gaaaagacaa ataaggatta tattttacca atctcaatac tttctggagg
     6301 aagtatttaa cgttaaatta attttttaat gtctataaaa ttcgttagaa aatacaattt
     6361 gtagatatcc aattgatttg taaacaaaaa ggagttgtca acttgtagtg acccccaaaa
     6421 gttagacttt tttggagtgg agaaatccac tccttttttc atgctctaaa atgatatttt
     6481 tacacaactg ttacggttgt tttacgaaat tggacgggac tttgccagtt taatttctgt
     6541 tttatccgtt cattattgta gtaatagata tagctatcga tggctgattt taattcctcg
     6601 aaacagttat aaactttccc gtgaaaaatc tcttgtttta ataagccaaa aaaattctcc
     6661 ataagtgaat tatctaagca gttccctttc cgagacatgc tttgaaagat tttgtactcc
     6721 ttcaatttgt ttctataagc tttcatttga tacgcccaac cttgatctga gtgaaacgta
     6781 cgacgatagg gacaatcgtt agtggtctgg atagcttcct ccaaaccttc cattaccgct
     6841 aatgcattgg gcttttcaga gatacgatag gataaaatct cactattgaa catatccata
     6901 aaaggatcta gatacaattt cttttgacgg ataatgcctt tggcatccac ttcatggtat
     6961 ttaaattcag ttgtatccgt cgtgattttc tgatggcaga tactggtgta gaaacgacga
     7021 tggatccgat ttttggctac tgctccgact tgtcctcgat aagaattgta gcgccgtgat
     7081 ttacgtgtgt atgtcgttac ttttattcct agcttcttga tcaggcgctg aactttcttt
     7141 ttattgatag agaaaccgcg gtttcttagt tcattggtca tccgtaaaca gccataatcc
     7201 ttgtgcttct cccgaacttt aagcatttcc gcttcaatct cttgatctgg atttttacgg
     7261 tccaatcgtt tttgccagta cataaagatt gccttaggga actttaaagt ttctagaagc
     7321 tcaactaatt taaacggtcc tcggaggctt gcgatgattc gtgcgattgc ttcattcgtc
     7381 gttgttgggc ttcctgtttt cgcagcttcc tcaattcttt taaaaaggca ttttcaattt
     7441 gaagggaacg gactcgtttc tccaactcct cgatgcgttc acgttcttta gacgttgctt
     7501 tagttgtaga ttttttctta tttttgggtt catttttctt agacaagata ggtggacatc
     7561 ctttcgtttt tgagagtcca tcgatcccac cctcacggaa ttgacgcatc caactagcga
     7621 tcaaattagg gttgttcatg ttaagtgtgt tggcaacttc acgataggac agctcacttg
     7681 ttagatataa ctctatcgca tccagcttaa attgaacaga atattttttc ttttgtcgct
     7741 ttcttactag accctcttca ccaaattctt gataggcatt gatccagcgc ttcacttgtg
     7801 atggagcctt aatggaatat ttctttgcca gataatcact tccgccttta ccatccaagt
     7861 aatcatgaac gatctttaat ttaagttcaa aagtatactt agccataaaa aataccgacc
     7921 tccaaaagtt agattttttg gtctaacttt tgggggtcgg tacaaaactt aaaagtttta
     7981 gaccctttta ttttcttaat gacttcagtc agttttgtgc gtaaagcgtg cgaaacaaga
     8041 aaaccgcgcg tttctacgcg tggaagaagt atctttagcg ataaaaactc ctttcgatat
     8101 actagtgagt ggctaccaac cgctcatcag atcgaaagga gttttttcat gtcaaatgac
     8161 gataaaagtt tagcatatac tcgttggaat tgtaagtatc atattatttt tacaccaaaa
     8221 tatcaacgaa aagtaatcta tgggcaatta cgaaaagatc ttggaaaaat acttaggaag
     8281 ttgtgcgaaa tgaaagatgt ggaaatcata gaagcacatg caatgctaga tcatattcac
     8341 atgcttgttc gagtaccacc aaaaatatgt atatccagtt ttatggggta tctgaaaggt
     8401 cgtagtgcgg tactaatcca cgagcagcac gcaaatctca aatataaata cggaaacaaa
     8461 tccttttggt cgaagagata ctatgtgagt acagtcggat tgaatcaaaa gacaaatgaa
     8521 aaatacatcc gtgaacaaga agctgatgat cgcattcgat atattataag taaaagagag
     8581 tacgtagaac catttaaaaa ataaagagaa gtaagtgagt gaagatgagc agttggcggt
     8641 cttttaaaaa tcccctttta aggggcagcg agtgtcgacc ccttctaggg ggattaaaaa
     8701 tacacccgtc ctcacgggtg tatgggttac tgatagactt gattttaact ggtgtcagaa
     8761 acttatgtcc agaaagggtg atgaaatttt ggcaatttac ggctattcaa gaattagcac
     8821 aagcaaccaa gattacaaaa cacaaattca aacattagaa aatgctggcg cagaaataat
     8881 cttcctagaa aatcactgga ttatcgaaca cctttggaag tatttttgag ttacgtaagt
     8941 attgatgatc tgtctaactt aatttgacaa ttaagagtat atttacttca gatggtaagg
     9001 caacaaaata aaatacaata ggcagatgaa ctaataaata aaatctgtat taaatacaga
     9061 taaattaata taaaaatcaa aaggatcata aacttttaac aagtgatgaa atgggtggaa
     9121 attgattctt atagacagtt tgtctcataa cattgaagta ttttgcaatt ttagcattga
     9181 aacacttttt taatcttcaa cgttgttctt ataaacaaga tgattgggaa tcttttgaat
     9241 taacactgta tagcttagaa caccctttat agaaaacatt cgcttagcaa tcttaataat
     9301 tccttgtgcc cgatgtcgtt tagattcagt aggttcctgc tcatcgatca tggaaaaaat
     9361 ttgaaggatc agatccttaa taaatttatt taagagagga tgatccattg cttcatccat
     9421 actcgataaa ctagtgataa tgagctacac gtttttcttt caagttgatc aatagcttcc
     9481 acgataaaaa tatctttaga taaaatcaag aaccgtagga actacaggga tatttggtca
     9541 ataagagcca ccgctattag taaaaaataa gctatcaagt atgctctcat cccaagaatt
     9601 gcctacttca atcgttaaaa accgttaggt ttagctaagt gggggtagtt cactcaatac
     9661 ttttatcaat aatagagcgc aaaacttttt ctcttaattc aactgaatca atattattgt
     9721 tttgttcata accatgaact aatagatcac aaatataaag ttgtgatatt tttccagcta
     9781 aagatccacc attaagaaat tcttcaatgg ctgtttgtaa aactaagtct gcagactttc
     9841 ctataggtga atgaatatag ttagttatag caataatttt tgcgccattt tttttagcta
     9901 ttttcaatga atcataagta tctttcgttt tcccagataa agaaaaaatg attactaaat
     9961 cattaactgt taataaagaa gcgacttggg cttgaaaatg agggtctagt acagcttttg
    10021 cttgaactcc tacgcgaaga aacatatttt ctaaatcaag actagtattt ccactagatc
    10081 ccactccaaa tatatatatg ttttttgatt gagtgatcat tttaatggct tcattcaatt
    10141 tttcttcgtc taaaagccta atagtggaat agagagcttc tgttaaacta ttagcaattt
    10201 catcatagta ctttacacta gtcggatgtt cttttttttg actaaagtct tcctttgcaa
    10261 tttcaatttt taaatcagaa aaaccagaaa aaccaatttt ttgacaaaag cgaataatag
    10321 tagcatcgcc aacttttgtg tttttcttaa tatcactcat cgtactataa ataatagttt
    10381 tcccagaatt taaaataaaa tcagcgactt ttttttcaga cttcgttaaa aatggataat
    10441 tttctttaat taaatagata taactcatac aaactcctct acaatttcaa cttttctcca
    10501 ttatactcca aaataataaa aaaaactcct aaatactcca tttttatctt tacttttatg
    10561 aaagcggatg ctatattgtt tttggagttg ttatccaaaa ataataaaaa attggagtgt
    10621 ttaggataat ggataaaaag aggtttttag atcttatttc aaaaggtttg attgtttcct
    10681 gtcaagcatt acctggtgaa ccattttaca aagaggaaga aagtttaatg ccttattttg
    10741 ctttggctgc tcaaagggct ggagcagttg gtatacgtgc aaattctgtt cgagatatta
    10801 ttgaaattag aaagtgtgta tcattaccaa tgattggaat tattaaaaga gattatccac
    10861 cagaatctcc attcattact gccactatga gagaagtaga tgaattagta gaaaccggtg
    10921 ttgaggtaat cgctcttgat tgcactctta gaaatcgaca tgatggacta tctataaatt
    10981 cttttattac taaaataaaa gaaaaatatc ctcatcagtt atttatggca gatatttcta
    11041 catttgaaga aggcaaaaat gcatataaag caggaataga ttttgtagga actactttaa
    11101 gtggatatac tgaggaaagt aaaaaacaag atgggccaga tatagatttg attaaaaaat
    11161 tggtagaaga agggatttgt gtaattgctg agggaaggat tcatactcca gaacaagcaa
    11221 aagagataaa aaaaatagga gttgcaggta ttgttgtggg tggtgctatt actcgaccac
    11281 aggaaattgc atcaagattt gttaaagcat taagttaggg ggagaaataa aatgtttaaa
    11341 aaattttcac aattaggacg cgctttcatg ttaccaattg ctatattacc agtagccgga
    11401 ttactattgg gactgggagg agcgttaaca aatgagagtg ctataaatgc ttatccattt
    11461 ttagatcaac cgtggttgca tacaatttta agtattatga gttatgctgg aaatgcagtt
    11521 tttgcaaacc tagcactaat ttttgcaatt ggtattgctg taggacttgc aaatggagat
    11581 aaaggaacag ctggtttagc tggtggagta gcttatctag tatatactgc aactattagt
    11641 gggtttcttg aacttttttc agcaaaagat gcaacactag atacaggtgt agtgggatca
    11701 attgttgttg gtggtactgt tgcattttta cacaatcgtt atagaaaaat tgaattacca
    11761 caattcttag gtttttttgg aggatcaaga tttattccga ttgtttctag tttagctgca
    11821 atcattattg gttctttctt ttatttaatt tggccaccaa ttcaaaatgg tttagtagtt
    11881 gctggtgaac aaattgccca aatgggtagt ttaggaactt ttttatatgg ttttttattg
    11941 cgtttaacag gtgctgttgg actacaccac accatttacc ctttgttctg gtatacatct
    12001 ttaggtggta ccgaaactgt agctggatca acaattgctg gagcccaaaa tattttcttt
    12061 gcccaactag ctgatccaaa ccacactggt ctgtttactt atggtactag attttttgca
    12121 ggaagatttg caacaatgat gtttggatta ccagcagcat gttatgcaat gtatcgtgca
    12181 attcctaaga aaaatcgtaa aaagaatggt gggctttatt ttagcggggc ccttacatca
    12241 tttttaactg gaattactga accagttgaa tatatgtttt tatttgttgc tccttggctt
    12301 tatgtgattc atgcattctt agatggatta tctttttatt ttgctgatat cttaaatatt
    12361 cgtgtaggga attccttttc aggtggtttg atcgactttt tattattcgg tgtacttcaa
    12421 gggaatgaca aaactaattg gttaaaatta attccttttg gtattgcatg ggcatttata
    12481 tatttctttg tttttacatt ctttatcaaa aaatttaagg tagctattcc tggaatggaa
    12541 gaagaatcag aagttcattc tgaaaaagta ataaacaaaa atacatcatt acatgaagag
    12601 tctcttatca ttattgaagc tcttggtgga aaagaaaata ttgagtctgt tgaagcttgt
    12661 gcaactcgtt tgcgtgttgc tgttacgaat ggggagatta ttcaaaaaca aacaataaaa
    12721 gacactggtg caactgctgt cttcgaagtg aagggtggca tccaagctgt ttttggaggg
    12781 aaagctgact tattaagtca agaaatcaat caaatattag gaaaagataa ttaggaggtt
    12841 tttaatatgt ttgaaatttt caaaaagaaa aaacaagaaa ctttttatgc accttgttcc
    12901 ggaaaaatca tttccatcac agaagtaaat gatcctgtct tttcacaaaa attactaggt
    12961 gatggttttg ccgtcatacc tgatggttca aaaatttatg ctccattggc tggtcgagta
    13021 acaaatatat ttcctactaa acatgctatt aatataaaaa gcaactcagg cattgattat
    13081 ttgattcata ttggaattga tacagttgaa ttacaaggag agcctttttc gatttgtgtt
    13141 caagaaaatg atgaggttac tcctgaaacc tcgttggttg atgtggattt tgacaaaata
    13201 ataaaatctg gtaaagatac ttctgttata gttgtttttc cggagaaaaa tcagttcaat
    13261 gatttaataa tagataatgg tcgaattgct catgggaatt catgtggaaa aatcactttt
    13321 taaaaaagtg tatataaaaa gctataaaat aagctaaata ggatatttgt caaatgaagt
    13381 tggtgaacag aaagaactgt tttctttctg ttcatttttt tattttatta gtaatgattc
    13441 cccatgccaa atcatgatcc gtaatttaaa gttataaaag cttcgaaatc cattggcatt
    13501 tcgttggaat accttgatat gattattcaa atataaaata tagagagaag agagagaggg
    13561 aagatattta taaagggcaa agttatgttt actcaactta ggaaatagaa atagatcaat
    13621 ttatattctc ttgtcaatcg aatatgaacc ttagcactat ataccaaata tagtaaaatg
    13681 ctgaaagatg
//
LOCUS       DAYYDS010000070        13429 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000070 DAYYDS010000000
VERSION     DAYYDS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.071"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACHA02_002089"
     CDS             complement(<1..914)
                     /locus_tag="ACHA02_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8670400.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACHA02_002090"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACHA02_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8670401.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACHA02_002091"
     CDS             2006..2176
                     /locus_tag="ACHA02_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670402.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="ACHA02_002092"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="ACHA02_002092"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8670403.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="ACHA02_002093"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="ACHA02_002093"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8670404.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /gene="dapA"
                     /locus_tag="ACHA02_002094"
     CDS             4440..5321
                     /gene="dapA"
                     /locus_tag="ACHA02_002094"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8670405.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /gene="dapB"
                     /locus_tag="ACHA02_002095"
     CDS             5318..6034
                     /gene="dapB"
                     /locus_tag="ACHA02_002095"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8670406.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="ACHA02_002096"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="ACHA02_002096"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8670407.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="ACHA02_002097"
     CDS             6751..7752
                     /locus_tag="ACHA02_002097"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8670408.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="ACHA02_002098"
     CDS             7749..9110
                     /locus_tag="ACHA02_002098"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8670409.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="ACHA02_002099"
     CDS             9070..10221
                     /locus_tag="ACHA02_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8670410.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="ACHA02_002100"
     CDS             10196..11365
                     /locus_tag="ACHA02_002100"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8670411.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="ACHA02_002101"
     CDS             11540..12919
                     /locus_tag="ACHA02_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8670412.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="ACHA02_002102"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="ACHA02_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAYYDS010000071        13383 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000071 DAYYDS010000000
VERSION     DAYYDS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.072"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..201
                     /locus_tag="ACHA02_002103"
                     /pseudo
     CDS             33..201
                     /locus_tag="ACHA02_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
     gene            complement(263..2074)
                     /locus_tag="ACHA02_002104"
     CDS             complement(263..2074)
                     /locus_tag="ACHA02_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8670413.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2071..2436)
                     /locus_tag="ACHA02_002105"
     CDS             complement(2071..2436)
                     /locus_tag="ACHA02_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8670414.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2599..2994)
                     /locus_tag="ACHA02_002106"
     CDS             complement(2599..2994)
                     /locus_tag="ACHA02_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8670415.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3012..3974)
                     /locus_tag="ACHA02_002107"
     CDS             complement(3012..3974)
                     /locus_tag="ACHA02_002107"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8670416.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3974..4186)
                     /locus_tag="ACHA02_002108"
     CDS             complement(3974..4186)
                     /locus_tag="ACHA02_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8670417.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4207..4905)
                     /locus_tag="ACHA02_002109"
     CDS             complement(4207..4905)
                     /locus_tag="ACHA02_002109"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8670418.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4925..5467)
                     /locus_tag="ACHA02_002110"
     CDS             complement(4925..5467)
                     /locus_tag="ACHA02_002110"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8670419.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5599..6603)
                     /locus_tag="ACHA02_002111"
     CDS             complement(5599..6603)
                     /locus_tag="ACHA02_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8670420.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6600..7589)
                     /locus_tag="ACHA02_002112"
     CDS             complement(6600..7589)
                     /locus_tag="ACHA02_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8670421.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7586..8392)
                     /locus_tag="ACHA02_002113"
     CDS             complement(7586..8392)
                     /locus_tag="ACHA02_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8670422.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8558..9514
                     /locus_tag="ACHA02_002114"
     CDS             8558..9514
                     /locus_tag="ACHA02_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8670423.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9591..10109)
                     /locus_tag="ACHA02_002115"
     CDS             complement(9591..10109)
                     /locus_tag="ACHA02_002115"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8670424.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10197..10346)
                     /gene="rpmG"
                     /locus_tag="ACHA02_002116"
     CDS             complement(10197..10346)
                     /gene="rpmG"
                     /locus_tag="ACHA02_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8670425.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10574..11020
                     /locus_tag="ACHA02_002117"
     CDS             10574..11020
                     /locus_tag="ACHA02_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8670426.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11215..13110)
                     /gene="asnB"
                     /locus_tag="ACHA02_002118"
     CDS             complement(11215..13110)
                     /gene="asnB"
                     /locus_tag="ACHA02_002118"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8670427.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4174 a         2833 c         2283 g         4093 t
ORIGIN      
        1 agatataaca aataataaat gaggtggatt caatgaaaaa atttgtatct ggaatattag
       61 taggaagtct ggcaacagcc gctgccgttg ctggtttggt tgcttctgtg aaaaaaaaca
      121 gtgatcgatc caattgacga aaaagaagca atgatcgaag aaaaccgtaa aaaagcaatg
      181 cgtaaacgcg tttctcgtta ataattattt aaaaatacgc atgaagactt tctaaaaaac
      241 agataagtca tcatgcgtat ttttatttgt cagtcaatat aagttgtgct attttatctg
      301 cctgtctcag attccatgga tctgtataag gtctagctac tgctgcagtt agccaatcca
      361 caccttcaac aggtattcca tagcaataaa agtcagactg gatttcccct gaagcatcaa
      421 tcacgcgatt cgtacctcgc tcaacatcta tagcttctga atgataactt cctgtattat
      481 ctgaaaatga aaaagggcga acaagtttgt tttccttcaa attttttatg acaggattac
      541 ttgaacgaga aaagcttgtt tgtggcaatc gagcttcaat caaatagcga ctgctgattt
      601 ctttatcttg tagagaaata acatagcggt tgtgcttttg ctgaatatca aatggcggtt
      661 cgatcaaatg aaaaataccc gcatctatca gtgctgctaa ttctttcgtc cgaatgattg
      721 gcggaccgat cgttaaaaag ctatttagat gagtaaacca tccccatagt atttctttgt
      781 attccttagc agaaaaaacg tgccattcaa tagcctgccg gattggatca cgccagtctt
      841 ttaatgcatc aaatgttgaa gcaaatgctc ctgtgcaatt gcctttttcc gcttctttta
      901 tctgcccttc aataaatatt cggctagctt ctgaaaaggg ctcattctga taggcaagag
      961 gagtttctaa atacgaccaa tcccatctat aagggattag tgctggatgc tttccgagac
     1021 actcctctga agagagggag agaaaatcat gctcaaaatt tttctttgga atcggcaagt
     1081 gatattcttc gatgattttc ttgtaataaa acaactcaac gtcttttttc agatagtcaa
     1141 aaaatgtttc gccagtcaaa aatcccgact gatgccattg ctccaggttt tcttttgtta
     1201 gtaaccgtgg ccatgccatt gctccgcctt gcttttgatt tcttcctcga gggtaataag
     1261 ggagccccct tcttgatcca ctgtatacaa tcggttcctt gcctgatgga tgatagacca
     1321 gttttccctt ttcgttctcg aagcgccctc ctcttgatac tgtaaacaat ccgacataat
     1381 caaaaaaact taagcctaga cctcttaaaa agacattttc tttaggcggt atcttcgaaa
     1441 gatctacatc tgcaggatta gaaggaagct gatacagtaa aacatttttc tttgcgtagt
     1501 ctacccacac tctttcttct tctgtctcat cattttccca gtgaccgctt gctaaaacaa
     1561 gctgatcgat agaatagctt tgctgatctg tctgtaaaag ataaccatct tttcttttgc
     1621 ttactttttt tactaaagaa taaacaaact gaatagaagc tggaaattct tcttgcaact
     1681 ttgagaaaaa ccagcgttga tagagtccgt ataaacatcg agtagattgc tgatcttttt
     1741 ccagacgatt gatttcttct ataattagtt gcttattttt ggttaccgtc tgttcgatat
     1801 aatctgtagc aggtccttta ctccattgaa acaagttggg tccctgtgtt accaatcctc
     1861 cactggacaa tgtttcatct gtaaaaagcg tcacttgttg agaaacagaa ttcattaata
     1921 actccggtgg ctgattcaac cgccaaactc gacctccagg accattggga tcgaaaagaa
     1981 aaagttcaag agaatgtctg tcctgattat tatggagaat cctttctgct acacttaatc
     2041 ctctaggtcc ggcaccaatg atccctattt tcatttgcat gcctccattt cgataaattt
     2101 tttcaataaa acagcatggt tctgatgttc attttttgca ccataaacaa aagtaatgac
     2161 tggatgttct ttcataagtt gactgagttt aaaaatagct tgcttctttt cttcttcgaa
     2221 aatcaattct tgctggtatt tctttgcaaa tgaagcaaat cgttctggta catggtcaaa
     2281 agttttgcgt gcttcattac taggtgcaat ttctttcaac cacagatcta actgcgcttt
     2341 ttcttttgaa attcctcgtg gccagaatct atccacgagt attctatagc catcctcttt
     2401 ttcagcatca aggtaaacac gttttaattt gatcatagtt gtttccttct ttctaccttt
     2461 tcgcttagct tatatatgta gataatagca caaaataaaa aaaatgaacg gcacaactat
     2521 ccattgcgcc gttcatgtga tcttggtcaa aatctaatga ttgattcggt ttttaaagct
     2581 gtcatacaat catctgtttt atactttttt acttttgatt tttccagacc atccgtcata
     2641 tccaccctta agtaaataaa tctcttgata acctgcttta cgtaatttat ttgctgtacg
     2701 gatgctcaaa cttttctttt gatcgtaaat atagacaggc tggtcttttc gaatagatcc
     2761 taatgaatct ttcaaaacag tgtaagggat gtttcttgca cctaaaatat gcccagcatc
     2821 aaacacatct ttttcgcgaa catcgattac ttgtgctttt ctcattcctt ctcgaaattc
     2881 ttcttctgtc aaagtcgttg ctgaacgttt ggccattact cgtagataga cttcccaaag
     2941 gatgattgca agcacaatca atagcaaaat gatattaatt acccataaaa ccatgtaatg
     3001 cctccgtttt tttaagcaaa tttcaaggct aatgaggcag caccaatgac accagcttca
     3061 ttaccaagct ctgctaattt gatttttgtt gattctctta cttgcgggaa agtaaattct
     3121 ttaaaatatt tctctacacg gctacgtaaa aattctcctg ctgccgatac tcctccacct
     3181 aacacgatac ttgacggatt taatgtattc cccaagtttc cgcaagctaa acctaagtag
     3241 aagcatacgc gatcaacaac catcaatgca aacggatcgt cttcttgagc catttcaaag
     3301 atatctttac ttgttacttc ttctccgttg tctagcattg ctttcagttt tgaatcccct
     3361 gcgtattctt ctgccaagaa acgacctagg cgtaccacac ctgtagcgct agatacagtt
     3421 tctaaacatc cgcgtttacc gcaagtacat tcgaaccctt caggatccac agtgatatgt
     3481 ccgatttctc ctccagctcc gctcacgccg tgaatcaaat ttccgccagc aacgatacct
     3541 ccgccaacac ctgttcctag agtaacgaat acaacgtctg gatcgttttc tccagcacct
     3601 ttccatcgtt cacctaaagc agcaacattc gcgtcattat cgatagcgaa tttgattcct
     3661 gttcctgcct cgatcagttt tttcacaaat tgaacttctg tccagtttaa gttatacgca
     3721 ccaataaccg ttccagcttc gctgtccacg cttcctgggg tacccatgcc gattccgata
     3781 aagtcttcgg ctttcatatt atataaattc aaacgatgat tgattgattc aatgatttca
     3841 ggaacgatat gcttaccatc atccaaaata ttggtatcga tgctccattt ttgctgaatc
     3901 tctccttctg gtgttaaaat tgcaaatttt gctgttgttc cacctaaatc aatcccaata
     3961 atcttcttgt ccattacaaa tttcctttct ttcggctttc ttcaattcgg tgctctcttt
     4021 ttaatatgcg ccacgctgtc atgtatgtat catgatcaat caaacggcta tcatacagat
     4081 tacgcaattc gatagtcatt aattcaatgt catagatgcg tgcacccaca tagataaaga
     4141 tgccaaattt tttgaacagc tgctgtacat catataagct ctccatcttt aagcacttcc
     4201 ttcatattat acaagtaaac catatttttt taaacccagt aaaatacata ttacaaaaag
     4261 aaatacaaaa actagtgtag aaagaatcct ttggtgtatc gaatagcttg tctgttgttt
     4321 cggtagaccc aaaacattcc ctaacactaa tccgccaatt agcccaccga catgtcccca
     4381 aacatctaca gaggtatcca gaagaccaaa aacaaatgtc aacacaataa ataaaagaaa
     4441 atgtctgact agctgctgga tcgctgggtt atgtttaaaa tgaaacccta agataaacaa
     4501 cgcaccgaac aaaccgaata tcgaagtact tgcaccacca gacagaacat tcgcttcgtt
     4561 aaaagcaaag cttgctgtat ttcccattat cccgctgaac atataaatca agaaaaaacg
     4621 ccaatggcca tagattgcct cgatttgctg ccccataaaa tacagtatca gtgagttcat
     4681 tacgaaatgc atcagtccaa aatggatgaa gattggtgtc actaaacgcc accattcatt
     4741 gaagtgaaca agataaggac catacattcc ggcataataa gggatctcaa gtcgcggaaa
     4801 taattccatc atgaggaaga ccgctgtctg tatccctaaa aaagcatagg tccataacgg
     4861 tcgtcttaac catgcacgca attttatttg ctgttgataa ttcatgaaaa cctcccattt
     4921 cattttagtc aataaacaat tgttggatct tctgatcaaa gctctccggt tgccattgtt
     4981 cattcaattg ttcacgaaaa acaaggctac atgttttttt atgaaaatta tttaaatacc
     5041 ggtcgtaata accaccgcca aaaccgattc gataaccggc tctattgtag ataatacctg
     5101 gtacaatgag gagatcgatc atctccggca aatagagctt ctctgattgt ggctcaagaa
     5161 caccaaaact cgaacgtaca agaacggact gttcatccat ttcataaaac gccatctgac
     5221 gtttcggcaa agttttaggt acaactattt tcttttgttc tttttctgct cgttggataa
     5281 ccggctgtgt atccagttca aattcattcg ataaagtcgt ccctatcact tttgctgact
     5341 tccagtactc gctggcgaat agctgtttat agattcgctg ttctttttcc tttttcttgc
     5401 cacttttggc cagatttttc agcttttcga ttcctttccg gcgtaatctt actttcaatt
     5461 ggtccaaaaa ctcacccttc cttctctcat aaaatggtat aggaaaaaaa gaaaaatagc
     5521 aatcgaactt tcaaataaat gcataaatag aataaaaaga agacctagaa atgattggcc
     5581 ttcctttttt aatgaatttc aactgttcag cgcgtgtttt tgtctgataa aaagtgcata
     5641 cagaaaatat ggcgcaccaa aaagggcaat caaaattcct gtaggtactt ccccacttgg
     5701 tagaatcact ctagcaacca tatctgcact aagcagaaaa atggaaccga acaaaccgct
     5761 cagaacaaag gtccagttat tttctttacg tacgactagc ttcgcagcat gaggagctag
     5821 tagtcctaca aaagataaac tacctgtcac cgctacacta ccgcttgcca aagcaacagc
     5881 taacaacaaa aggatttttt gacttttagc cacgttaagg cctaatcctt gcgcgcgttc
     5941 ttgaccaaat gatagcaata caatcctctg tgcataaagt cctcctagcg gaattaagat
     6001 aaccgcccat ggaagcaatg cccaaacata aggccagctc gcaccccaga ttgatcccgc
     6061 taaccatgca ttcacaaatc cataattttc cttagataat tggatcgtgc atagcaatgt
     6121 taatgcagaa aggccggcat taacggcaat accggacaat aagattcgat tcatacccaa
     6181 aaagccattt cgatcatagg ccgcaagata gacacagcaa gcagctaaaa caccacctag
     6241 aaaggctaca actagcaata gaaacggcag cgaatagtgc gaaaagaaac ctaaataaag
     6301 catcactaaa aatcctgctc cggcattaat gcccaatata ctagaatctg ccaattcatt
     6361 tcttgttact ccttgcaaga tataaccaga aacgccaaga caagctcctg ctaataaact
     6421 gatcagtaat cgaggaactc gaaattctct aacaatcagc gccaaagaag atgattcttt
     6481 ccctatgaac gtggcaaaaa gctgatccag gtaaataaaa ggcgtaccaa tcattaaact
     6541 acctatagac aggatgacaa gtacaaaaag caagaaatag aacgtccaat atattttttt
     6601 caccgttgtt ccctccttac ttgtatcagt aaaaacggaa cgccaatcaa tgcaatgact
     6661 aatccaaaag gtgtttcaaa agggggattg atcatcctcg ctgtaaaatc cgccaacata
     6721 accaatccac cgccaactaa aaaagagaca ggtagaacat aacgatagtc cgttccaacc
     6781 attccgcgaa caactggcgg aaccaacagc ccaataaatg aaacaggtcc gactaacgaa
     6841 acggcaatgg ctgctaacac tgcaactaga cagaaaacaa tcaaccgcgt tcgtttagga
     6901 tgacttccaa gagctaatgc atgtgtttca ctcaaatgaa tcaaagatat tgcttttcct
     6961 aatgaaaaag aaaccaaaac agcaagtata tataaaggaa acaatactgc taattgtttc
     7021 catgtgatat tggcagctcc gcctacaaac caaaaagcaa gatcctgctg cagattaaag
     7081 aattgggtaa gtgcttggct gatagcagta aaaaaagaac taatagctac gccagctaaa
     7141 atcagctgta aaggattcat gcccattttc gaaaactgca aaaaaccaaa tacgagcaaa
     7201 gttacagcaa aagaacctgc caatgacatt agaaacacag aaaaatcaga agctttaggc
     7261 caaaaagcaa aactaagggc cattgccaac gaagcacccg cgttgattcc tagtaagcct
     7321 gaatctgcta aaggatttct tgtcacccct tgcatcaatg caccgctcaa tgcaaaacaa
     7381 ctccctacaa gaaatgctcc cgccacacga ggcaaacgaa tatttcgtat gatttggtga
     7441 ctttgttttg atctatcaaa atcaaacaac gcctgccaaa aatctgcttt ttccacagtc
     7501 gctgcgccat ttgaaagagc aacaaaaaaa ataatcaaaa gaacaaaaca gaccaaaagc
     7561 aggagtctat ttttcttcat agtcctcata attgatcctc ctgctctttt ggcaacaaat
     7621 cataggttag aaaaactggt ttatttgttc ttggaagcgt gacaatcgtt gcatcaatct
     7681 caaatatttt tttcatacac tcttttgtga tcacttcttc aggtgtacct tctgtgatca
     7741 acttaccgtt tttcattgct aataaataat ctgaaaaacg tgaagcatga ttaatgtcat
     7801 gaatcgacat caagatcgtt gtttgtttca tttgattgat ttcagtcaat aactgtagga
     7861 tctctaattg gtgtgccgga tctaaaaatg ttgtcggttc atccaagatc aagatatctg
     7921 tttcctgagc aagcgccata gcgatccaga cacgctgggt ttgtccacca gacagcgttg
     7981 cgatatcccg catacgaaat ccagctagac ctgtagcatc caacgcccac tcaattgctt
     8041 catgatcttg cgtgtccatg ccactgaaca gtttttgata agggtatcgt ccgtaagata
     8101 ctacttcttc tactgtcata cctgtagggt gttcactagt ttgtgctaat aatgcaattt
     8161 tttttgcaag ttcttttgaa ctgtatgtag agatatcaga gttattgaca atgatctttc
     8221 ctgatttcac aggtagtata cgggtaaaac ttttcaataa agtagatttt ccacttccat
     8281 ttggcccaat caaactagtg attttccctt caggaatcgt aacagacatg ttttcaatga
     8341 tttttttgtt ttcgtagccg gctgcaatgt cagctgctct taatctcgtc atttcaccca
     8401 tcctttcgct gaaaatcatt ctcaattatg acgctgtttc aacatttcgt caagcgctac
     8461 tttcatatct atagccaaat gaagcggttg acagcgttcg aaatatcagg aataataacc
     8521 aatgagaaag attctcaatt atgttaggag gaaagaaatg aaattaaaaa caaaagtatt
     8581 cacattatct agcatattgc tgctggctgg agtactgagc gcttgctccc cctcttcaga
     8641 taaagaaaca gctacaactg ccaattcgac gacttcatcc aatagtactc atacagtcac
     8701 agatacactt ggacataaag tagagattcc aaacgcgccc aaacggatca tcggcagtta
     8761 cttagaagac tatctgattg cactagggga aaaacctatc gcccagtgga ctgttggcag
     8821 tggttccatt caagattatc tgcaagatga tttaaaggat gtcccaacga tttcttatga
     8881 ccttccttat gagaaagtgt taagctttga gcctgatttg ctgttgattt cttcttccgc
     8941 tactgtcgaa ggcgggaaat acgaacaata tagcaaaatc gcgccaactt atgttgtaaa
     9001 aaacggaaca gatgtcacat gggaagaaca actaaaagat gtcggcaaag ctttggcaaa
     9061 agaaaaagag gcagaaaaaa tcatttccga ttatcaaaaa tccatcaaga aaactcgaga
     9121 agaactagca gataaaatcg agaacaagac agctgcagtg ttgtgggtga ccaacaactc
     9181 agcctttatg gtcagcaaaa atcggtcaag tggaagaatc gtatacgacg atctggaatt
     9241 tggtgttcct gatctagtgg aagaggtctc caaaaaagcc acttctgact ggtctgctgt
     9301 ttcctctgaa aagctagctg aattagatgc tgactacatc atccttgtga acagtgacaa
     9361 agatgctgcc atgttcaatg aaccaagctg gaagaatcta aaagcagtga cagacaatca
     9421 tgtactgcaa ttcggacctg aatctagttg gctatataac ggacctattg ccagtcagga
     9481 aatggtcaat gatatcaaag aaaaactaaa ttgattttaa ctaaaaaaag catcttcacg
     9541 attagtcgtg agatgctttt tttcttatta acaaactaga cgcatagatt ttattcttct
     9601 tttgaagcaa ccctattttt tactctttcc catttttgaa aacgatgggc atatagattt
     9661 tcttgatccg ttatcccctc atactcctct ataagggcga agtctgaaaa atcaatattc
     9721 ccaatatacg tatcgccttc aaacgtgcta tcgatcaatg ttcgccagat ccgaccagtg
     9781 taaggaagaa gttcttgaaa aatccgacta ccacccgaaa catagatatc ttctgagatc
     9841 gtttgactat acgacaaaag ttcatcgatc gaatgcatta cttccgcatt ttctttcgtg
     9901 cgaaaatttg gttgcgttgt caacacgatg atatggcgat aaggaagtga aagatccccc
     9961 attccttcat aggtctttct tcccatcact aaaattcgat ccattgtatg ttcgcgaaag
    10021 aaacgcatat catttggcaa tcgccatggt aacaaaccat ttttgccgat cagaccattt
    10081 ttatcttgtg cccacatgga aataaacata ttatccgctc cttatgattt tagatgtttt
    10141 gagataaaaa aagtctcaat tctttgacca gcaaggaatt gagaacttta aaaacattat
    10201 tttgtttcac ggtgcaaagt aactttgcgt tcgcgtgggc aatatttttg tttttccaaa
    10261 cgatcaggat tgttacgttt gtttttgctt gttagatagt tacgttcttt acaagaagta
    10321 cactctaaag tgatgtttac gcgcatgttt ttccctccca tttttacaag aatcctctaa
    10381 gatgactctc agacttgatt atcttaccat gattttttct agattgctag tgtttttcac
    10441 gattttgtta agtaaaacta cttgacgata aatacaacgg ttttacttct caaacaaaat
    10501 ctttttcttt ttgtataaat acccttacac ttgtttatat agattatgtt ttttaataaa
    10561 gggggatact aatatgccat ggaacatgaa ggactttcct gcatcaatga aaaacttaga
    10621 taaattgaca agaaaaaaag caatcgacat cgcaaatgca ttattagatg aagggtaccc
    10681 tgataaccgt gcgatcccaa ttgccattga tcaagcaaaa aaatggtatg aagatgctag
    10741 tgaatcagaa aaacgaactt tcgaaaaaga aaaaaatcca agcaagacgg atgaacacga
    10801 tacgaatcct cgtgctggaa aactgcttga ttcagatgtg atcgtagaat atgaggaaga
    10861 acaatggatc gtgaaatcta aaggggcaaa aaaggcaagc aaccattttg atacaaaaaa
    10921 agaagcaatc gaaaaaggaa agcaagtagc acaaaacaaa gaatcaactt tgttcattta
    10981 taaaaaagat gggacaaaag aaaaagaaat cgattattga acatatgtaa aggggttgtg
    11041 acgaaaatcg tgtcacaacc cccttatgtt cgaaacaatg gtgttcctct gccaactccg
    11101 aaccataagt cataatatct ataaaacggg aagagctgtt ttttgaaatt gtttcttatc
    11161 actcagtgtt aaacggtagg agaaaacctc ttttttattg atatttgatt ttcatcaaac
    11221 agtcacatag tcgattgctt ctgcttgcgg aatcgtttca tctacaaagt agactttata
    11281 ccatgtcaag aaactgaaga ttgtccagat ttttctttgc tcttctttac gtccttcgtg
    11341 atggtcatct agcaacttca tgattttttc ttgatcaaaa aattcttttg caaaatctgc
    11401 ttcgaataat gctttgactt gttcgtagcc gtgttcttct ttcaaccaag ctttgattgg
    11461 cacagggaat cctaatttca cacgattcga ccattcttcc ggaagatgtt tgttcgcagc
    11521 ttgtctgaaa atatcttttg tgttgttttg attcaataaa aacttacttg gtatttgttg
    11581 agccaatttc atgacttcga tatccaataa aggtactcgg acttccaaag aacttgccat
    11641 agaaagttta tctgccttta acaagatatc ttttggcatc cattgatgaa gatcgacata
    11701 ttgcatctta ttgacttcat ctttgattcc ttctgttttc tcgtaatgaa cagtcatgat
    11761 ttcatccaca gatggtgctg tttgatattt tggtgttaac acttctgatg cttctgattc
    11821 ttcgaacact cgagcatgac cgataaaata ttcttttgca ggagcaagtg attcatataa
    11881 atggatccgt ccatggaagt ttttcatttt tccgatttta cgtcctaaat tatagcgtgt
    11941 tccttttggt aattttttca atccttgagc aatcacacgg atgaattttg aattcgtatg
    12001 gaatccatac gtttggtatc ccgcaaacag ttcatccgcc ccttctccag attggactac
    12061 ccgcacactt tgtgaagcaa gatttgctaa aaagtacaaa ggtacgcaag aaggatttga
    12121 atcaggttca tctaagtgat actggatcaa agggaatgct ttgaacgaca tgtctccgtc
    12181 gatcactgct gctgtattat ctaagtccaa tagttcggtt agttttctgg cttcaatcgc
    12241 ttcattatag gttttatcat caaacccgat tgagaaagaa tgatctggtt tcaatacaga
    12301 ggttacataa ctggaatcaa ccccgctgga taagaaagaa ccaacttcta catcactgac
    12361 cgtatgtgct tcaatagaag cttgaacggt ttcgtcgatt ttttcgatcc attcttgtcg
    12421 ggaatgcgtt tcttttgttt caaaatctgc atcccaatat tgctgaatgt ctatttttcc
    12481 atctttatac gtataataat ggccttcagg caaacgatag acatctttga agaatgtttc
    12541 gccgtttaat ggagaatatt ggaaagtcat ataaggtttc aacgcttctt tgttcaattc
    12601 tttgttaaaa tcaggatgag gcagaaaact tttgatttct gaaccaaaca taaatgttcc
    12661 gttcatttct gcataataat atggtttgat cccaaagtgg tctcttgcac cgaataactc
    12721 attcttttca ttatcccaaa tagcaaaagc aaacattccg cggatctttt gcaataattc
    12781 tgttccccat tcttcgtaac catgcaaaag gacttctgta tctgcatgtg tttgaaaaac
    12841 gtggccagct gcaatcaatt cttctctcaa aggctggtaa ttatagattt cgccattaaa
    12901 aataatgatc tttgtccgat cttcattgta aataggctga gtaccgccct ctaagtcaat
    12961 aatacttaat cttctgaatc ctaaagcaac atgtttatcg atatattctc ctgaactatt
    13021 aggtccacgg tgaacgattc gatccatcat tgtattaata attgtttttt tgttgtcctt
    13081 gtcgttaaca aaacctacaa taccgcacat ttaactttca catcctaata taaaattact
    13141 gtttatttca ttcatatgca tcttatcata gaataaatag cggacgaata ttttctctct
    13201 tacaatttcg tcacgtgaaa acaaaagatg aaagtttctg tttttacaga aaataaagtc
    13261 ccaatatcta atttaccatg ttttcttttt tgaggctacc ttattctctt cgtgttaaaa
    13321 aaatattagt ggatgctatt tccaaaaaaa ccagcaaaaa aagacacgac ctaaagatcg
    13381 tgt
//
LOCUS       DAYYDS010000072        13383 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000072 DAYYDS010000000
VERSION     DAYYDS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.073"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..2011
                     /gene="yfcC"
                     /locus_tag="ACHA02_002119"
     CDS             272..2011
                     /gene="yfcC"
                     /locus_tag="ACHA02_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8670428.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2168..3352
                     /gene="iadA"
                     /locus_tag="ACHA02_002120"
     CDS             2168..3352
                     /gene="iadA"
                     /locus_tag="ACHA02_002120"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8670429.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3463..3666
                     /locus_tag="ACHA02_002121"
     CDS             3463..3666
                     /locus_tag="ACHA02_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8670430.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3767..4429)
                     /locus_tag="ACHA02_002122"
     CDS             complement(3767..4429)
                     /locus_tag="ACHA02_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8670431.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4442..5944)
                     /locus_tag="ACHA02_002123"
     CDS             complement(4442..5944)
                     /locus_tag="ACHA02_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8670432.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6492..8171
                     /locus_tag="ACHA02_002124"
     CDS             6492..8171
                     /locus_tag="ACHA02_002124"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8670433.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8298..8777
                     /locus_tag="ACHA02_002125"
     CDS             8298..8777
                     /locus_tag="ACHA02_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8670434.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8996..9676
                     /locus_tag="ACHA02_002126"
     CDS             8996..9676
                     /locus_tag="ACHA02_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8670435.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9684..11015
                     /locus_tag="ACHA02_002127"
     CDS             9684..11015
                     /locus_tag="ACHA02_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8670436.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11030..11992
                     /locus_tag="ACHA02_002128"
     CDS             11030..11992
                     /locus_tag="ACHA02_002128"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8670437.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12103..12441
                     /locus_tag="ACHA02_002129"
     CDS             12103..12441
                     /locus_tag="ACHA02_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8670438.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12520..13227
                     /locus_tag="ACHA02_002130"
     CDS             12520..13227
                     /locus_tag="ACHA02_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8670439.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4291 a         2346 c         2923 g         3823 t
ORIGIN      
        1 ttttcaatga ttgtaaaatg aaaaaaacag gacgagattt tctctaatct aaaaataaaa
       61 aaagaatttt aaggaaaaac agagaaataa aatacttaat ataaatggaa agagaaaaaa
      121 ataatcatac ataggagaaa aaaaacaaaa tagaaaaaag tgattttcta ataaattatt
      181 catgtggaaa tgtcattttt actgtataat tcatttgatt atccattttt tattcatcaa
      241 aattactgaa aaaagaaagg aagaaataaa tttgaagaaa acaaattctt caggaaaacc
      301 gaaacgcgac ttcactaaat tatcgacacc ccatacgtat gtcattattt ttggtgtagt
      361 catctttgca tggatactga cttttgtcgt tccagcagga aagttcagca cgcaagatat
      421 tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact gttttacgtc aagattcctt
      481 ccgttatgct tacgagctgg ataagtcgta tgtgtttgat caattagaag aactgcaaga
      541 tcatccagca gaacgtgaaa aactggatgt tccggaaaaa ggattggaaa aagtcattgc
      601 tgacggagaa aaaaatctta cgcaggaaaa actagatgaa atctccttga cagatgatgt
      661 gctttacgat cagtacggcg aaaatatcta tgatacttct aaaaaattgc ataaaacagc
      721 caaaatatgg ggaacagatg attttggcgg ctttggtttc ttaaattttg tttttgaagg
      781 actagtatct ggagataagt atggatctgc tgtagggatc gctgcattga tccttgttgt
      841 tggaggtgct tttggtatta ttatgcgtac cggagcgatc gacgcaggta tctatgcgtt
      901 tatcagtaaa actaagggac tggagcgttt agctttaccg ctattattct ttgctttttc
      961 attcggtggc gctacattcg ggatggcaga agaggtaatt ccattctcaa tggtgatggt
     1021 tccatttgtc attgcgttag gatatgactc gatcgtagca gtaaccgtga cttatgtcgc
     1081 atctcaagtc ggaaatgcga cctcttggat gagcccgttt agcgtagcgg tggctcaagg
     1141 gattgctgga atccccgtgt tgtctggtgc gacattccga ttgattatgt gggtagttgt
     1201 tactgcatta gccgctggtt atatgatgat atatgcagaa aaaatccgta aaaaaccaga
     1261 aagttcgttg acttacaaat cagatgatta tttccgttct catatcaaaa agacatctga
     1321 tgaaaataaa aaattcatgc ttgggcataa attgatctta ttagagatgt tagttgtcct
     1381 tgtctggatc gtttggggcg tgactcaaaa aggctattac attccagaaa tcgcttctca
     1441 attctttgtg atgggattag ttgctggaat cattgctgtt cttttcaaat tagatggaat
     1501 gaaagtcaat gacatcgctt cttctttcca atctggtgca gctgatttag ctggtacagc
     1561 aatcgtagta ggtatggcca aagggattct tcttgtcttg ggaggatctg atgcatctgt
     1621 tccttcagct ttgaatacga ttttgcatgg gatcgggacg gcgttgaccg gtgttccagc
     1681 tgtcattggt gcttgggcga tgtacatctt ccaaagtttg ttcaacttgg ttgttacatc
     1741 aaattcagga caagcagctt tgactatgcc aatcatggct ccactggcag atttagtagg
     1801 tgtatccaga caagtagcag ttcttgctta tcagcttggt tcaggtttta tggacgcttt
     1861 tacacccgta tcagccagct tgattggtgt gctaggtgtt gcacggatcg aatgggcaaa
     1921 atgggcaaga ttccaaatca agatgcaagg attcctgttt gttttaggaa ctattttcat
     1981 catgattgcc attgcaatag gtctgcagta gtactggaga cctgtaaata aaaaagtaat
     2041 ccaaacagta gataaggact ccaagaaccg tcattttacg gtaaaatcaa aggagtaaag
     2101 atcctatcgt ttggattatt ttttaagatc ctatcatcag ttatacttaa agaaagagga
     2161 aaaatgtatg aaaatcattc gacaaattga agtgtttgca ccagattact taggaaaaat
     2221 ggatgtactg attgccggtg ggaagatttt ggctgtagaa gaacagctag aaggcggcta
     2281 cgaaggagtg gaagtagaag aactttcagg tgagggaaaa gtactaaccc cgggtttcat
     2341 tgactgtcat ttccatattt taggtggagg aggagaaggc ggttatcaaa atcgaacgcc
     2401 agaagtcaca ttgagccaat tgactacagc tggtgtaacg acagttgtcg gttgtttggg
     2461 aacagacgga gaaggtagag atatgactgc gttgatcagc aaagcgaaag gtctggaagc
     2521 agaaggaatc tccacttatg tatacgaagg ttcgtaccga ttgccggtaa aaacagtaac
     2581 agattctatc atcaaagact ttttgacaat tgataaaatt atcggaatcg gagaaatcgc
     2641 agtatccgat caccgttctt ctcagccaag ttttgaagag ttcgctcgtg cagtagcaga
     2701 tgctcgtgta ggaggcatgt tatccggaaa agctgggatc atcaatgtcc acttaggcgg
     2761 aggaaaaaga aagctagagc tattgacacg aatcgtagaa gaaacagaaa ttccgatcac
     2821 acaatttttg cctactcatg caaatcgcac accagaatta tttgaagctt gcgtggcatt
     2881 tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa gatcctgatt tttgggagaa
     2941 gacagatggt gaagttcgct tcagcaaagc actgaaacga ttgatcgagg aagatgtttc
     3001 tttagataat ttcacgatga cctcagatgg acaaggaagc ttgccttatt ttgatgaaaa
     3061 caatcatttc ctagggttag gagtaggaag tgccaaggca ttgctagtag ggatcaaaga
     3121 agctgtgcaa aaagaaagta tccctttgga aatcgcctta cgagctataa ccagtaatcc
     3181 agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa attggtgctg atgcagatct
     3241 gtgtatcttg gataaagaaa cgcttgatat tgatacagtt atcgctaaag gagaaataat
     3301 ggtccaagaa aaagaagtca aagtatgggg gactttcgaa aaaagttttt aatcaacagc
     3361 tctaactatc ttttttagaa aacgagcgtt ctgtttgtct gtatctgttg ttgatgttac
     3421 cctgaaggta gatcaataaa caaactaaga ggaggaagca caatggcaga tttaaaagga
     3481 cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa aagaagcata cggcaaagca
     3541 acagacgaca agagcaaaga aacagaaggg aaagcacaat cactaggtgc tgacatcaaa
     3601 gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt tttctcgaaa agacgaaaaa
     3661 aaataaaaac cacttattac taattgaatc aagcaaataa aaaatccgaa ccatatctgc
     3721 gagacagata tggttcggat tttttatttg atcattatcc tgttttttaa acttcgattt
     3781 tttgaaattt tttatttttt agttcaaaga cttcatcaca gctttttgct acttcaatct
     3841 catgagtcac gatgataata cagcgatttt gttcatgggc gatttcttgg aaaagtttga
     3901 cgatatcttg tgaagttgtt tcatctagat ttccagtagg ctcatccgct acgattagtt
     3961 gatgttcaca gcagatagca cgaacgatag cgactcgctg ctgctgacct cccgaaagtt
     4021 tggtcacctg tttttttgct aatatttcat caattcctac acgagccagt cgatctaaag
     4081 caacttgtcg ataattctct tgcttggtct tagtgatttc cattgctgct atgacgttgt
     4141 ctagagccga taaataagga agcaagttgt aagcttggaa aataatcgag acatcatttt
     4201 tacgataact tcttaacccg attttgttca gtgaacgatc ttgatagtat acctttcctt
     4261 cttttggtgc atctagtcca gaaataagcg aaagaaaagt cgtttttcca gaaccacttg
     4321 ctcctaaaat agcgtacatt tttccagctt cgaaattcag ttctacatct ttataaagcg
     4381 catcttgcgg atcttgatac caaaatccca attgttctgt ccgtaacatc aaattcctcc
     4441 tttagtttaa taggattttt cttgggttta atcttaatat ccctatagaa gctaaaaaga
     4501 cagaaaacag actgattcca aatgctactc cggctaatag tcccaattgt gcaggtttga
     4561 cattgatttc caattcgctg acttgatcag aaacagcaaa tggattattc gtggttcctt
     4621 gttgaccatc gcgttgaccg ccttgttctt gtctgttacc tggcatttgt ccaccgccag
     4681 gagcttgctg gttttgtgtc gtggtttctg tctgttggtc aagtagctgc tgtcccacgg
     4741 cattcgcgac gacgtttccg ctaagcgaag caactcctaa tgcaagaatc atacaaatag
     4801 ccacttcggt aaacaattgg agaatgattt tacttctgga ttctcctaaa gatagcaaga
     4861 cgcctaactc atgccgacgc tcccgaatgg taatcataat gatcagagta agtatgataa
     4921 taccggctac agccactagt aaaacaacat ttttagaaaa acttgccacg ttgtttaaag
     4981 gttccaacat tgactgatac atggaatcat ttgattgtaa gctaaatgta tctgtgtcga
     5041 tcagcttctc agcttctttg agaaactcat ccattttatc tggatcagat agagtgtaaa
     5101 tagcagaatc tatcgtgtta tcgtcagatg tcccatttag ttcatttgca aaagtgtaag
     5161 aagtgtaaag tgtattcgac ggattcatga agttgaattt catacccata ctgccgcttg
     5221 tttcgctgga ttcataaatt cctttgatcg tcatttcata agtggtatct tcatcttcag
     5281 tactcgtgat tgtgaaagta tctccgactg ccaggtcatt tgtttctgct agagtagaat
     5341 caatgactac attgtttgtg cctttgtcgc tgtcatctat tccttcacca tccgtgattt
     5401 ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc tgacacacca ctgatttgaa
     5461 agtctgcttg agtcatttgc ggattcccgc ccattcctcc agcaggttca ccgcctgcat
     5521 cagtagtttc tgaactgtct tcacttgaat cagaactagt gatggcagtt attccatctt
     5581 ttgccatagc tgaggcggaa ctttcaaagg aataactctt tacattatct aattcagcta
     5641 ttttttccgc gtccgttaag gaaactggtg taagagaaaa actgcctgga tctggacgtt
     5701 cttcagtggt agaaccgctt gaagaagttt gttcctgttt tttaaatgaa gcttctcgat
     5761 tggcacttaa agtcactgtt gctccaactt ctttttgtgc ttgattcgct gcttcttctg
     5821 cagcgcttcg aatcgtgagg ccagctagca caaaaataag aatggctgtg aacacggcaa
     5881 acaataacaa tgaacgtccc cattttactt ttgtactttt ccatgcacgt ttcataaaat
     5941 tcatattttg catccctcct agtagatgca tttgataata gacaacctat cttaaagcta
     6001 gcttaaatct tatgctgttt gcacaccatc aacatgaggt tctgtatata taaaaagtca
     6061 ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta tgtttcaaac cgacgaagaa
     6121 gaaatgaaag aggaatagca aaagaaatcc tagatgctac agagggattt ttacgtgcat
     6181 agttgttcta acaattgctg atactagcga tttagtttat ttgttgtata ataatgggga
     6241 acagctgaat acttatataa gtattctggc tatagcgttt cgcaaaagat ccattttgtt
     6301 aatgaagtat atccaaaata aaatgcccat ccaactgtca gaacgcggat gtgctggtaa
     6361 ttttcctgct gattctccat aagttgagaa atagtgattt tgcatatgct tcattggaat
     6421 ggctcatata tttagataaa gaagaaaatc gacatcttta aacttggatg tcataagaag
     6481 aggtgaagtt tgtgagtacc attaaaatta tcccattagg cggcgttcgt gaaaatggaa
     6541 aaaacatgta tgtcgcagaa gtgggcgagg atatttttat tttagattgt gggctaaaat
     6601 atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc tgattttacg tatttagaag
     6661 aaaacattga tcgtgtagct ggtgtctttt taacacatgg acatgcagat gcgatcgggg
     6721 cacttcctta cttgttgtca aaaatttctg taccagtatt cgggacagag ttgacgatcg
     6781 agttagcaaa attgaatgtc agtcgtaatg aagcagccaa aggatttaag gatttccatg
     6841 tggtagatga gcatacagaa atagactttg gtgaaacagt agtgagcttt ttccgtacca
     6901 ctcatacgat accagattca gtggggatca atctgaaaac aaaagaaggt agtatcgttt
     6961 atacgggaga ttttaaattt gaccaaacgg ctatcccaat gtatcaaaca gattatgcac
     7021 gattggcgga aatcggcaaa gaaggagtct tagccttact aagtgaatcg gcaaatgcag
     7081 aaaatcctca gcctgtagct tctgaagccc aaatagcaga tgaagtattt gatacgatca
     7141 aatattggga agggcgtatc atcgtcgctt gtgtagcaag taatttgcaa cgagtgcaac
     7201 aagtattgga tgcagcagaa aaagcacaac gaaaagttgt cttgactggt caagatttcg
     7261 aacgaattat tcgcacagcg atgcgtttag aaaaacttca attaccaagt gaagatctgc
     7321 ttgttacgtt gaaagacatg aaaaactatg atccagaaga actgatcatt ttagaaactg
     7381 gccgaatggg tgaacccatc aagtctctgc aaaaaatggc aaacggtaca caccgtaatg
     7441 tcaagattga agaaggagat ttggtttata tcactactac gccaagcatc gcgatggaaa
     7501 ccatcgtggc gaaaacggaa gatatcattt atcgagctgg tggaacagtg aagttgatct
     7561 ctgagaatat gcgcgtttct ggccatgcca atcctaatga cttgcaatta atgatcaatt
     7621 tgatgaaacc aacatatttt gtgccagttc aaggagagta tcgtgaatta gctgctcatg
     7681 cagatttggc tcatgcagta gggatgccat acaaaaatat ctatattact ggacgcggag
     7741 acgtgctgga atataagaac aagcgtatga gtgttgcagg aactacaagt gcagaaaatg
     7801 taatgattga tggattaggc ataggtgata tcggaaacat tgttttacgt gatcgaaaaa
     7861 tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat cagtcggcga gaaaagaaaa
     7921 tcatttcaaa acctcaaatt acttcacggg gattcgttta cgtaaaagct agccgtgatc
     7981 tgatgaaaga aagcagcaat atgattgaaa caatcgttga gaaacattta gaaagcaatg
     8041 attttgagtg gagcaaatta aagcaagaca tccgagatca attaggccgt tacttatttg
     8101 aacaaacaaa acgccgccca gtgatcttgc cagtcattat ggaagctact caacgtcgaa
     8161 ataaaaaata aacaaaaaga gtaagatgaa cctctctata gagtgctcgt cttgctcttt
     8221 ttttgcgaaa aacaaataat ttttgaatac agcatccctc gctttataat aaacgataga
     8281 ataaagggag tgaatcaatg atgagtcaaa caaaagtcga acgttattta gaagaacaaa
     8341 agatcagcta tcaaccactt gattttagtc atgtggtaga agaatctttc acagaagaac
     8401 tacaaaaaag agggattgat ccagctttga tatgtaaaac tctcgtagtg aaaggaaaca
     8461 aaactggggt gatcatcgca ttgattcctt tgaacgatca cttagattac aaaaaaacga
     8521 gacaagtgac gaaagatcgt aaagttggtt ttcctggaat ggattttgta ttaatgcata
     8581 caggatatcc ccacggagca aacaccccta ttggaataaa actcgcacat cctgattatt
     8641 gttttttagc agacagttct ttaaaagaaa aagaagaagt aattgtgtca agcggtgaaa
     8701 tcgggagaag ccttcggata aaagtagccg atttagaaaa gatcgtccat cctatttacg
     8761 ctgatttaat tagttaaggg acgagaagag aagctaagca gagcagagac ctcaaacttg
     8821 agattatatc tgcttggtac ttggtgcatc ctgtattatc tgttacaatt gctttgtaca
     8881 tactgtacta tcaatccaat gaaaatatat ttagcagcta tattttttca ttgatgtgca
     8941 agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa cgcgaggtgt aaaatatgaa
     9001 aattacgtat catggacatt cctgtttaac aattgaaatg gaaaatggcc aacgcgtgat
     9061 gattgacccg tttattacag gaaatccatt gacagactta actgcagaag aagtaaagac
     9121 agattggttg ctgattactc atggtcatag tgaccacata ggagatatga ttcctattgc
     9181 aaaagaaaat caagccacaa ttatcagtat tgttgagatt gctgattatg cgaacagcag
     9241 aggagtagat tcattcggta tgaatatcgg tggaaaacat gcgtttccat tcggcgtggt
     9301 aaaattcgta catgcacagc atagttcaag ctatgaagta gacggtatcg ttcagtatat
     9361 gggcgagcct agcggaatta ttattcaagc agaaggaaaa acgatttatc atgcagggga
     9421 tactgcttat ttcagtgatc taggattgct tgcagaagaa ttcgatattg atgtcgcttt
     9481 cttgccgatt ggcgataact atacgatggg gccagaagat gcagcaaagg ctgcgcaaac
     9541 aattcgtgcc aagaaagtag ttccaatcca ttacaatact ttccctgtca ttgaacaaga
     9601 tcccgaatct tttgtagagt tactggaaga tagtgaagga aaaataatga gcgtctcgga
     9661 aacgctggaa gtgtaggcag aaaatggcga caaaacatga tttgatttta gaatatattg
     9721 aaagtttacc tgttggcaat cgaatttctg taagaagcat cgcaaaagaa ttgaatgtca
     9781 gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa tgtcggacta gtttcaacaa
     9841 ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa aaaacatatt gaacgtttga
     9901 catttggcga ggtcgtcagg atcattgaag gggacgtttt aggcggggcg gcaggattag
     9961 acaaggtctt gaataaattc gtcatcggcg cgatgactaa acatgccatg gaacgctata
    10021 ttacgccagg atcactaatg atcgtcggca accgggcgga agtccaaaaa ttagcattgg
    10081 aagatggcgc cgcagtattg atcacaggag gattcaatac gactgctgag attgcaaagc
    10141 tagctgatga tcttgaaatg ccgatcttac gcacctctta tgatacattt acagtcgcaa
    10201 ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa agatatcatg cttgtcagtg
    10261 atatctatat gccagctgaa aagactcatt atttgcagca gacagatacg atttctgatt
    10321 atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc agtcgtcaac cgccatcatc
    10381 gtttgatggg gattgttacg gcaaaagatg ttttagggaa atcgccaaac cagatcatcg
    10441 atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac aatgagtatt gcctctgtta
    10501 gccatcaaat gatctgggat gggctagaag tcatgccggt cgtttccgat gacttgacat
    10561 tggaaggctt gattacccgg caagatgtaa tgaaagcgat gcagcttgtc cagcgacaac
    10621 cgcagattgc agatacgatt tcggatcaga tatctggaaa tatccatacg attgatactg
    10681 accgtgaagg aaatcgtttg gaaaacccta agttcaaatt cgtagtagca ccacaaatgg
    10741 tcaatggcgt tggaaccatt tcttttggcg tacttagcga aatcatttct gatgtcgcac
    10801 aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat tgaacaagtg aacctacatt
    10861 atttgcggct gatccaattg gaaagcgaat tagacattcg tccacgtgta ttggaaatcg
    10921 gcagacgttc cgctaaactg gatatcgaag tgtttattga aaacaccatc gtttccaaag
    10981 caatcgttgt ttgtcaagta atggaacgtt cttagaaagc aggagtgaaa tgacagtaca
    11041 gcaagaaata gtagatacaa taaaaaaata cgataaaatc attatccaca ggcatttgcg
    11101 ccctgatcct gatgcattag gatcacaagt aggattagcc gaactattac gggaaagttt
    11161 cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa gggcttcgct ttttagcaga
    11221 aatgcaagaa gttgaagatg tcttctacca aggagcgcta gtcatcgtga ctgatacagc
    11281 taattcaccc agaatcagtg atcaaagata tcagttggca gaaaaactga tcaaaatcga
    11341 ccatcatccc aatgatgacc cttatggaga tcttgtgtgg gtaaatacaa aagcaagtag
    11401 ctgtagtgag atgattgcag aatttgcttt gatgtttcct gaatctttaa agatgaacgt
    11461 taaggctgca cggttacttt atgcaggaat cgtcggagat acagggcgct ttttgtatcc
    11521 ctccacgacg tcccgtacat tagagattgc ttcgatattg agaaattatc catttgatgc
    11581 aagtgctttg aacagagaga ttgaacaaat gccgatgaaa gtcgctaaac tttccggtta
    11641 tctttatcaa aatatccagg tggatgaaaa tggtgcagga agaataatcc ttccgcaaga
    11701 aattcttgat aaatatggaa tcgatgattc agagacagct tcgatcgttt cacttcctgg
    11761 agtgattgac gaagtgctgg cttgggggat ttttgttcag cagccttcag ggtattaccg
    11821 cgtacgttta cgttctaagg ggccgatcat caatgaattg gctaaacgcc atcatggagg
    11881 aggccatcca ttagcaagtg gtgcgaatgc aaaagattta gcagaagtag aggaaatcta
    11941 taaagagatc caagatatat gccaatcgta taagagcaga aaaacagaat gatacttcgt
    12001 tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc ttttcaagat cttctttttc
    12061 cgttacaata gtgctcacta aagattttaa aggagaaaga agatgacatt accaaattgc
    12121 ccagaatgtg gctcagagta cacatatgaa gatcgaggct tgttgatttg cccagaatgc
    12181 gggaacgaat ggaacaaaga agaacaagaa actgtcatag aagacggatt agtggtatta
    12241 gatgcaaatg gaaaccgtct gttagagggc gatagcgtga cagtgattaa agacttgaaa
    12301 gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag tcaagaacat ccgtttagta
    12361 gaaggcgatc ataatatcga ttgcaaggta gaaggatttg gaccgatgaa actgaaatcg
    12421 gaatttgtga aaaagaatta aagaatattc aaggtctttt cacattcttt tcctactact
    12481 aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa tgtatggtta tggaatgtat
    12541 ggttttttag atccgacatt tcttttggtc attcttggat tagttatttc cggaattgcg
    12601 tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag tgagaagcaa aaacaatgtg
    12661 actggtacac aagcagcaca atatatatta caaagtcaac agataaataa tgtcggtgtt
    12721 caacaaattg ccggagatct cacagataac tacaattcag gtaataagat gttgagtttg
    12781 tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag gtgtagcagc tcatgaatgc
    12841 ggacatgcgg tacaagatgc ttcgggttat gtcccattga agattcgtgc agcacttgtt
    12901 ccagcagcta actttggttc gatgatttcg ttccccttga ttatggtagg tgtattgctt
    12961 agctggaact ctacgttaat caatatcggg attttcgctt tttctttagc attacttttc
    13021 caattggtga cattaccagt cgaatttaac gcgtctcggc gagcaatcca aattttgagt
    13081 gaaggcggac tactgacgga agaagaagta ccgatggcaa gacacgtatt atttgcagca
    13141 gcactgactt atgttgcagc agcgctttcg acattcctgc agctgttgcg cttgatctta
    13201 ttgttcggcg gcaaccgaag agattaaaat aaagaagttg tgaaatcagc gctttgatct
    13261 gagctaatac cgagagttag ctgattgaat accatttatt tggaataagg gctctttgtc
    13321 aataaggact gatgggttgt acaaaatcaa atctgggtca gaatgaacct cattctggct
    13381 cag
//
LOCUS       DAYYDS010000073        12898 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000073 DAYYDS010000000
VERSION     DAYYDS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.075"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..712)
                     /locus_tag="ACHA02_002131"
                     /pseudo
     CDS             complement(14..712)
                     /locus_tag="ACHA02_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            791..967
                     /locus_tag="ACHA02_002132"
                     /pseudo
     CDS             791..967
                     /locus_tag="ACHA02_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="ACHA02_002133"
     CDS             complement(1143..1712)
                     /locus_tag="ACHA02_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8670440.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="ACHA02_002134"
     CDS             complement(1786..2109)
                     /locus_tag="ACHA02_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670441.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="ACHA02_002135"
     CDS             complement(2263..3075)
                     /locus_tag="ACHA02_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670442.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3402..4277
                     /locus_tag="ACHA02_002136"
     CDS             3402..4277
                     /locus_tag="ACHA02_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFM8670443.1"
                     /translation="MSKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4291..4614
                     /locus_tag="ACHA02_002137"
     CDS             4291..4614
                     /locus_tag="ACHA02_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670444.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4611..5363
                     /locus_tag="ACHA02_002138"
     CDS             4611..5363
                     /locus_tag="ACHA02_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8670445.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="ACHA02_002139"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="ACHA02_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ACHA02_002140"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ACHA02_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8670446.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="ACHA02_002141"
     CDS             complement(9827..12013)
                     /locus_tag="ACHA02_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8670447.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ACHA02_002142"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ACHA02_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8670448.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="ACHA02_002143"
     CDS             complement(12235..12675)
                     /locus_tag="ACHA02_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8670449.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..>12898)
                     /locus_tag="ACHA02_002144"
     CDS             complement(12749..>12898)
                     /locus_tag="ACHA02_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HFM8670450.1"
                     /translation="FIWHFEENNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKI
                     ESKNN"
BASE COUNT         3630 a         2698 c         2291 g         4279 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaa
//
LOCUS       DAYYDS010000074        12805 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000074 DAYYDS010000000
VERSION     DAYYDS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.076"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..917
                     /locus_tag="ACHA02_002145"
     CDS             180..917
                     /locus_tag="ACHA02_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8670451.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            931..1746
                     /locus_tag="ACHA02_002146"
     CDS             931..1746
                     /locus_tag="ACHA02_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8670452.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1759..2406
                     /locus_tag="ACHA02_002147"
     CDS             1759..2406
                     /locus_tag="ACHA02_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8670453.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2422..3078
                     /locus_tag="ACHA02_002148"
     CDS             2422..3078
                     /locus_tag="ACHA02_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8670454.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3240..4061
                     /gene="racE"
                     /locus_tag="ACHA02_002149"
     CDS             3240..4061
                     /gene="racE"
                     /locus_tag="ACHA02_002149"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8670455.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            4064..5419
                     /gene="rph"
                     /locus_tag="ACHA02_002150"
     CDS             4064..5419
                     /gene="rph"
                     /locus_tag="ACHA02_002150"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8670456.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5420..5938
                     /locus_tag="ACHA02_002151"
     CDS             5420..5938
                     /locus_tag="ACHA02_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8670457.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            6045..6545
                     /gene="cbpB"
                     /locus_tag="ACHA02_002152"
     CDS             6045..6545
                     /gene="cbpB"
                     /locus_tag="ACHA02_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8670458.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6825..7745)
                     /locus_tag="ACHA02_002153"
     CDS             complement(6825..7745)
                     /locus_tag="ACHA02_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8670459.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7871..8923
                     /locus_tag="ACHA02_002154"
     CDS             7871..8923
                     /locus_tag="ACHA02_002154"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8670460.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(9033..9788)
                     /gene="fabI"
                     /locus_tag="ACHA02_002155"
     CDS             complement(9033..9788)
                     /gene="fabI"
                     /locus_tag="ACHA02_002155"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8670461.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            10008..10448
                     /gene="fabZ"
                     /locus_tag="ACHA02_002156"
     CDS             10008..10448
                     /gene="fabZ"
                     /locus_tag="ACHA02_002156"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8670462.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10563..11324)
                     /locus_tag="ACHA02_002157"
     CDS             complement(10563..11324)
                     /locus_tag="ACHA02_002157"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8670463.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11513..12607
                     /locus_tag="ACHA02_002158"
     CDS             11513..12607
                     /locus_tag="ACHA02_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8670464.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(12693..>12805)
                     /locus_tag="ACHA02_002159"
     CDS             complement(12693..>12805)
                     /locus_tag="ACHA02_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8670465.1"
                     /translation="SVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         4147 a         2203 c         2685 g         3770 t
ORIGIN      
        1 tcctaaggaa aaaacaagga aagaggataa aataactaat agattagaaa tggaagaaat
       61 atttatggga gaataaacgt tctaagaaac tttacaaatt actaaactat cagatataat
      121 gtattttatt atacatcttt aaaagaaatc ttactaaata ataatgggag tgtaattcta
      181 tggcaatgat taaatttgat cacgtggata aatattatgg caagttccac gcattaaaaa
      241 atatcaatct cgaattcgag aaaggagaag ttgtcgttgt cattggtcct tctggttcag
      301 gaaagagcac gatgcttcgc tgtatcaatg gattagagac gatctcttca ggtaagctgc
      361 tgattaatga tactgacttg catgataaaa agacaaaact gacagaagtc cgaaaaaaca
      421 tcggcatggt tttccaacat ttcaatttgt acccaaataa aactgttctg gaaaacatca
      481 aattagcacc aatcaaagtg ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga
      541 aatttttgaa aactgtcaat atgttagata agaaagattc ttatccttcc atgttatccg
      601 ggggacaaca gcaacgggtg gcaatcgcca gaggtttagc gatgaatcct gagatgctgc
      661 tgtttgatga acctacttct gctttggatc cggaaatgat tggtgatgta ttagacgtta
      721 tgaaaaaatt ggctcatgac ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg
      781 ccaaagaagt ggcggatcgt gttatcttca tggctgatgg agaagtattg gaagacagca
      841 gaaatgtgag agactttttc gaaaatccaa atgaagtccg agcacagcaa tttatcagta
      901 aagttatcaa tcattgatag gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt
      961 ttccttttag gcattgcttt tcttgcacta gccggatgta aaggaaatgg gttggcaaat
     1021 gaagatattt tagagcgaag caaatctacc aatgaaatca tctggggtgt caagtatgat
     1081 actcgattat ttggaatgat ggatattgag agcagaactg tacaaggatt tgatgtagat
     1141 attgcaaaag caatcactaa aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa
     1201 gtcacttcta aaactcggat tccattattg aaaaacggaa atatcgatgc aattattgca
     1261 acgatgacga tcacagatga acgaaaaaaa caggttgact tttcagatgt ctatttcgat
     1321 gctggccaag ctttgttggt aaagaaagga agccaaatca aaagtgtaga tgatttgaat
     1381 gcttctacaa cggttctagc agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat
     1441 gcaccagatg caaaaatttt agaattagaa aactatgcag aagcatttac tgctttacaa
     1501 tctggtcagg gagacgcgat gactacggat aatgcgattt tgttagggat tgcagatgaa
     1561 aatccagagt atgagttagt tggtggaacg tttactaatg agccttacgg gattgcgatc
     1621 aacaaagggc aagaaaactt cttgaaagct gttaaccaag cgctagagga aatgcatgca
     1681 gatggcacat acgataaaat ctatcaaaaa tggtttccaa atgaaactga aggaaaagta
     1741 gagtaagggg gagaggcaat gagcgaattg atacaaactt atggaccaat ctttgtcgac
     1801 gggttcaaga tcacattgtt ttcgagtgtc ttagcacttg tgtttagttt gattatcgga
     1861 acgttaatgg caatcttcca attatcaaag aataaagtag tgagcgcctt agcaaaggca
     1921 tatgtagaat tttttagaaa cattccatta ttaatcatcg tgatgttttt ttatgttgtc
     1981 gtaccgattt attggtttaa tctagatgga ttccaagcag gtacgattgg attaacgatc
     2041 tatacatctg cttttattgc tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt
     2101 caaacagaag caggactatc ttctggtttt acctattcag aaatcatgcg ttatattgtt
     2161 ttaccgcaag cattcaaaat cgttatcccg ccattaggca accaatttat caacctcgta
     2221 aaaaactcat ctattttagc aatcgtagct ggtttagatt tgatgtatca aggagacttg
     2281 atcgctagtg aaacattcaa taccttcgat acgtatatca tcgttgggtt attttactta
     2341 gttatcacgc taccgttatc ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt
     2401 tcatagaagg aggggaaata catggatttt agcggcgctt tttcatggat gaatatccgt
     2461 tttttattgg aaggattaaa ggttactgtt gaagtttcat tgttttcgat catttttagt
     2521 ttcttgatcg gtggtctgac aggtgtgatt cgttttgcga atatcccttt cttttccaaa
     2581 atccttggtg tagtgattga tattatacgg aatcttccat tattattgat cattttcttc
     2641 acttactttg ccttacctca aatcggcatt cagatgaaca tcttctggtc cgctgtcgca
     2701 gcattaacga tttttgagtc agctatgttg tcggaaattt tccgagctgg actcaatgct
     2761 gttccaaaag gacagatgga agcaggatta tcaacgggac tgacttatgt agaatcgatg
     2821 cgaacgatcg tgttaccgca agcatttaaa tcaatgatcc cggcaattgt gagccaattg
     2881 atttcactga tcaaagatac ttcattagca gtaatcattt ctttacctga actttctcat
     2941 caggcacgta ttatttatgg acagaacacc aattatgtgc ttccgatgtt catcatcatg
     3001 acacttatgt acttcgtggt atgttacgcg ctttctctgc tttccggtta cttggaaaag
     3061 agacggtaca gttattaata gatgattctt ttagcggttg tggcaaacta aagtcacaac
     3121 cgcttttcct ctttccattc gaaaaaaaga gatttcgatt gttttaagca agtaagagca
     3181 cttgaaaatt cacgctaaaa agtacacaat agggaggata gaatttattg aaaaaaagga
     3241 tgatacgatt gacagataat cgccctatcg gatttattga ttcaggtgtc ggcggcttga
     3301 ctgtagtaaa agaagccctg aaacaattac cgaatgaaaa tattttattt gtaggagaca
     3361 cagcacgctg cccatatggc cctagacccg cggaacaggt aatccagtat acttgggaaa
     3421 tgacggatta tctggtggag caaggaatca agatgctggt gatcgcctgc aataccgcaa
     3481 ctgcggtggc tttagaagaa atcaaagctg ctctttctat tccagtcatc ggtgtgatcc
     3541 ttcccggtac tagagcggca gtaaaaaaaa cacaaaataa acaagttggc attatcggta
     3601 cgattggtac ggtaaaaagt caagcttatg aaaaagcact gaaagagaaa gtaccagaat
     3661 tgactgtgac aagtcttgct tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc
     3721 attcatcggt ggcgaaaaaa attgtggcag aaacattagc tcctttaacc actaaaaaaa
     3781 tcgatacatt gattttggga tgcacccatt atccattatt acgccccatc attcaaaatg
     3841 taatgggaga aaatgttcaa ctgatcgatt ctggagcaga aacagtaggt gaagtatcta
     3901 tgctgttaga ttatttcaat ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt
     3961 tttatacaac tggctctgcc aaacttttcg aggaaatagc tgaagactgg cttggaatcg
     4021 gacacttaaa tgtagaacat atcgaattgg gaggaaaata aggatgcgtc atgacgggag
     4081 aaaagcccaa gagcttcgca aactggagct tcaaacaaat gttttcaaat atccagaagg
     4141 atcggtagtg atccgttttg gtgatacaac agttatttgt tctgcaacaa tagaaaattc
     4201 tgttccccct tttttgagag aaacaggaac aggatgggtg acagcagaat atagtatgct
     4261 gcctcgagca acgagtacga gaaatcgtag agaaagcagc aaagggaaac tttctggtag
     4321 aacaatggaa atccagcgtc ttattggtcg ttccttgcgt gcagtagtag acttggaaaa
     4381 attaggagaa cgtagtatca tcgtagactg cgatgtgatc caagcggatg gcggtactag
     4441 aacagcaagt atcactgggg catttgtggc attgaaatta gccattgaaa aacttttaag
     4501 agaaaaagaa ttgtcagaag atccaataaa agaacatctt gctgcagtta gcgtaggaat
     4561 cttaccagat gggacttgcg tcacagattt ggattaccaa gaagattccg cagcacttgt
     4621 agacatgaat cttgtaatga cagaatctgg aaaattcgtg gagattcaag gaactggaga
     4681 agaagcaacg tttgatggtg aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat
     4741 tgaagatttg atcaaagagc aaaaacatgc tttgctaaca gagtttgctc aaaatgatga
     4801 gcggatagaa gaaacaaaaa caatcatcat tgcgacaaga aatccaggaa aagcagaaga
     4861 gtttcgaaat atgttcaaag aagcgggata tcatgtaaaa acattacttg actatcctga
     4921 attgccagat gtggaagaaa ctggcagcac atttgaagaa aatgcccgat taaaagcaga
     4981 aacgattgca caattgcttg atcagccagt cttagcagat gattcaggtt tgaaagtgga
     5041 tgctttaggc ggtatgccag gaatctattc tgcacgtttt gcaggggaac aaaaaagtga
     5101 tgcggggaat aacgccaaat tattatatga attgacagat gtgccggatg aaaaaagaac
     5161 agcacaattc cattgtacgt tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga
     5221 agcggaatgg cccggtcgag tagcccgtat accatctggc gaaaatggct ttgggtatga
     5281 tcctttattt atcccagaag gtaagaaaca aacagcagca gaattatcga gcgaagaaaa
     5341 aaacaagatc agtcatcggg ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg
     5401 gctggaagga gagagataga tgcggtattt agtagtaagt gataatcatg gagacagaga
     5461 tatcctcgtt gacttagtag atcgttatcg ttctgaagtg gatatctttt tccattgtgg
     5521 tgattcggaa ttagaagctt ctgacccatt atgggataca ttccaagtgg ttcaaggtaa
     5581 ttgtgattat ggaccaggat tcgaacagaa aaaagtgatc caaactggtc aggatacagt
     5641 attcatgact cacggtcatt tatcgaatgt ccgatttggt ttgacacagt tagcaatcga
     5701 agctcgagct gcaactgccg atatggtgtt ttttggtcat acccatcaaa taggctgtga
     5761 gatggatcag aaaatcctgt ttttgaatcc tggtagcatt tctcagccta gaggcccgat
     5821 ccaaatccca tcatatgctc ttatcgaaag tttagatgac caaatttctg ttcaatacta
     5881 taaccgtgcc catcaggcag ttgaagagtt ggccttttct ttttcgagag agcgctgaat
     5941 aaaaaaactc taatttttaa taaaaataag ctttatttta tgaattttgc atccggtttc
     6001 ggtaaaatag tagtgttgaa tgaaatagaa atggaggcca tcagatgatt ggacctatag
     6061 taagagaaat gctgctggaa aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa
     6121 atgttatgta tcatcatcca ttgtcccatg gtttacttgt gttatcgaag gtcggttatt
     6181 ccaaaattcc tgtattagga aaagatgatc actttgtagg actagtaagt ttatcagatg
     6241 tggtcaataa aatgattgac ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg
     6301 ttgctgatgt gacagaaaca gatgttccga caattgatga aaattgggaa ctagaagaag
     6361 tactccattt gttagtcgat tcgccttttt taccggttgt cacagaagat cggattttca
     6421 aaggaatcat tacaagaaaa gagctgctta aagccgtaaa cttcatggtg catgaattag
     6481 aacgtcgtaa tatattgtct ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga
     6541 gctaaagaac gagaaactaa aagataatca aaaaataagt agaaaaaaca gcgtaccagt
     6601 tttgacttta tcttgaaaaa gaggctggga caaaagcgtt taactccaag aaataagaag
     6661 gaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggagttg
     6721 cttctgcttc cgccgtttat acgtgtttag agcgtgggac aaaagtgttc tttacttttg
     6781 tcccacgctc tttttagtga gattcgttgc atcgtttgtc caaattattt tccgacagca
     6841 attggactgt tcggaatcgt gaaaccagaa gctgtcaatt ctttgacata ggagctcaag
     6901 aactcttctt ttaaggcata ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca
     6961 aaattgctgt ttccaagatc cactaatcca aagatagaag gttcagtttg gagctcttct
     7021 tggtattttt cagctaatgt acgatttact cgatcaatga tctcatagat tttgtcatat
     7081 ccttcttcag gaacaatccg aatatctaaa agtacttgca tatttgcacg agagagattg
     7141 ctgatcgtgg tgatgtttcg attcgggatg aaatggacag taccatccgt tgctttcagc
     7201 tgaagggtac ggataccaac agatacgacg gttccttcaa tcgttaagtt tgctagcttg
     7261 atgtagtccc caacatccat ctgttgttcc atgataataa aaaatccggt aataacatca
     7321 ctcatgaacc cttgggcacc aagaccgatt gccacacctg caattccggc accagccaaa
     7381 agtgatccga tcggcacacc gatagcagat aaaattgcat agataaaaaa gaaaccaagg
     7441 gtataatgaa aaatagtatt taaaagacta tgtaaagtct ttaagcggct ctcgtttaga
     7501 atggcttttt tagaatactg attaaaagac ttatcaatca gatattttcc cacacggtga
     7561 agaatccaaa aaaggacgat caaaaaaatc agatataaac ttttttggat aaaggcagcc
     7621 aaaatggcgt cccaatcaat cccgtcccaa aaccgctgaa acgcatttac tttgctgaca
     7681 gcttgatctg tcaatgtctc gccagaagag tctgtcgtac cggcagttgc taataatgaa
     7741 aacataaaat cctcttttct aaaatttgat tctttctgta ttgtaccaag aagttttcaa
     7801 aaaaggaaat agcaaaaata actataatga aggaaaaaag ctgaaaataa aatgacaaat
     7861 aatatggata atgataaaat gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga
     7921 ttggaacaat ctaggttttt cttatatcaa aactccttgg cgttttattg ccaaatggaa
     7981 agacggacaa tgggaagaag gagaattgac agaagataat tacctgacga ttcatgaagg
     8041 ctctccagcg cttcattatg gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa
     8101 agacggcaag gtcaatctct ttcgacctga acaaaatagt cgacggatga atcaaagtgc
     8161 tagacgattg ctgatgccaa tggtccctga aaagatgttc gttgatgctg tcaagcaagt
     8221 ggtaaaagca aatgaagatt ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg
     8281 ccctttactg attggggtcg gtgaaaacat tggtgtccat ccagctccag aatatttatt
     8341 tgtcgtcttc tgtacgccag taggagcgta tttcaaaggt ggactaaaac caacgaattt
     8401 tattgtatct gattacgatc gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg
     8461 aaattacgca gcgagtttac ttccaggaac agaagcaaaa gaactggatt actcagattg
     8521 tgtttatctt gatccagcca cacatacgaa aatcgaagaa gtaggtgcag caaatttctt
     8581 tggaattaca aaagacggaa aattcatcac tccgcaatcg ccctctatct taccaagtat
     8641 cacgaaatat tccttgttgc agttagctga agaacgacta ggactgcaag caattgaagg
     8701 agatattttc attgatgatc tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc
     8761 tgttatttct ccaatcggtg ggatttatca caataacaat cttcatgtat tttatagtga
     8821 aacggaagta ggaccagtaa caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg
     8881 agacatagaa gcaccagaag gctggattga agtcgtggaa taatttcaaa gtaatgagtt
     8941 gaaaaaaacg taaaaagaaa aaatcaggaa gagactttaa aaagtttttt cctgattttt
     9001 atatgtttta ctttaaagaa tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg
     9061 tagataatat caccgatgat tccgctggaa agtgggctga ttaagaaagc acaagcatta
     9121 ccgacttctt cgatcgtcac accttgctga tcaggcgtac gagattcaga taactggatc
     9181 agtttttgat agtctttaac acctgtgaca gctaatgttt tgattgcccc agctgagatt
     9241 ccattgaccc gaattccttt aggcgagaac tcagctgcca aataacggat agcagcttca
     9301 agagaagctt tcgcaatacc catcatgtta taattcggga ttgcgcgttc tgaacctaga
     9361 taagtcatgc taacgatccc gctgtttggt gcaagaaacg gttgtgcgta ttttgataca
     9421 gcaattaaag agtagctgct gatatcttga gctaattgat agccgtctct ggaaatatca
     9481 gacacatttc cggacaattc ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg
     9541 atctccccag catattcacc gatggcattc attgcttttt cgatgctttc gtcgctagaa
     9601 acatcacatt ctacagtgaa tgcttcttcg ccgaccaatt tgactaatga ttttttcatt
     9661 cgttcatttt ggtaagtata gataatttct gccccttgtt tcaacattgc ttcagcacaa
     9721 ccccaagcaa tgcttttttt gttagcgacg cccataatga cgattttttt tccttctaga
     9781 aatgacataa tgactctcct ttgtttctga taaaaatttg atagtcgtaa aaaaatagag
     9841 aataatagct aaaataactt cttgatttct taattattag tcaaaatgtt ttgattgtca
     9901 aactattttt tcttaaatta aaaaaagatt gctaaatatt taggaatatg atactttgat
     9961 agtcaaagta atgaaaatgg caattgcctg aaagaaggag tttaattatg tcttacgtat
    10021 tatctgctac tgaagtaatg gaattgattc ctaatcgtta tcctatttgt tatattgatt
    10081 atgtagatac tttagaacca ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg
    10141 aagatttttt ccaaggtcac tttcctgaca atccagtgat gccgggtgcg ttgattctag
    10201 aaacactggc acaagcaggt tcgatactta tcctaaaatc agaagagttt ctaggaaaaa
    10261 gagcttatat cggtgggatc aataaggcca aattccgtca aaaagttgtt ccaggagatg
    10321 tgctgaaatg tcattttgaa atcaccaaaa tgaagggatc agtcggtaca gcgttatctg
    10381 cagcatatgt agatgataaa aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa
    10441 atgcttgact ataaaacctg atatagcaaa acaaaaggaa gcttacttgc tttttatgta
    10501 aaaagaagac gcctgaatcc tagcatacgt tgtcatctat gcaggattca gacgttattt
    10561 ttttattttt gttttgcttc ttgcagtata ttgattttat cgatttttac ttcttctttt
    10621 ggtttgtctg aactatctgt ttcgatattt gcaatagcat ccacgacatc cattccttca
    10681 atcacttggc cgaagacagt ataatggcca tctagtgaag gataaccgcc gtttttataa
    10741 gcgtcgatga ttgcttgagg atagtcgtct tttaaaagac catcatgcat atcatctgta
    10801 ttttgaacga taaagaactg gctgccgtta ctgttaggat cttgtgagcg agccatagat
    10861 aacgcacctc ttatattata aaggtggttg ttgatctctg tttcaaatcc ttctccccaa
    10921 atgctttccc ctccagtgcc atcgccttta ggatcgccac cttggatcat gaaatctttg
    10981 atgacgcgat ggaaagtcag tccgttgtag taaccgtctt ttgcatgtgt cataaagttt
    11041 tcaaccgctt ttggtgcgtg ttcgggaaat aatttgattt tgattgtgcc tttattcgtc
    11101 accatttcga ccagatcttc attttcggcg acatctttcg tcaattgtgg taattccaat
    11161 gcgttcaaat cgacgctact ggcagtcgtg ctttccgcag tagatgtact ttttgaactt
    11221 tctgtagacg aactagaaga agtcgtgttg tttgcagaac aaccagcagc aaacagcaat
    11281 gctgctgcta aactaatact taaaataact ttatttttct tcataactgt tcctttccaa
    11341 tttactattt tgttatcttt aatgataaca aaatagaggg aaaataccta cttaaattat
    11401 tggaatttat catttatttt aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt
    11461 gctataatgt aagtggatac aaaaacgaaa agatgcagga ggaaatggta atatgggaaa
    11521 attaggaatc tcgatttatc cagaacggtc aacatttgaa aaggacaaag cgtacttgga
    11581 tttagctcat aagtatgggt ataaacgggt ttttaccagt ttgctgcaga tcaatgatga
    11641 taaagaaaaa gttctttcag aatttaaaga agtagttgat tacgcgaata gcctaggcat
    11701 ggaagtaatg gttgatatca atccagcgtt atttgaacag ttggagattt cttacgatga
    11761 cttatctttt ttccataaga tgggtgccta cggggtccgc ttagatatcg gattcacagg
    11821 agcagaagaa gcaaaaatga cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag
    11881 ttcgggcacg aattacgttg acaacatcat gagttattcg ccaaacacgg ataatttatt
    11941 aggttctcat aatttttatc ctcatcgata ctctggttta ggatacgaac actttgtttt
    12001 ttgttcagaa aaattcagaa aatataattt gaatacgatg gcattcgtca attcgcaatc
    12061 tgctgagttt ggcccttggc ctacgcaaga tggcttgtgc acgctggaag accaccgaga
    12121 tcttgaaatt gcgacacaag tgaagcattt aatccttaca ggattgattg atgatatttc
    12181 tattggaaat gcgtacgcaa gtgaagaaga attgaaagaa atggcagaag catttaacgc
    12241 ggattatcca acattgaaag tagatacgga agaaggtatt acagaaaacg aacgaatctg
    12301 cttgtttgat aaccttcata gttatcgagg ggatcgctca gaatatatcc ttcgttcaac
    12361 gatgactcgt gtatactaca aagataaaga tttcccgcct cataatacgc gagatatgca
    12421 tcatggggat gtactgattg acaatgaagg gtacggtcaa tacaaaggag aaacacaaat
    12481 cgccttgaag gatatgaaaa acgacggacg tgtcaatgtt gtaggaagaa tctccgatga
    12541 cgaattattc ctccttgact ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa
    12601 aaaatagata attagttatt gcagaaataa tccgctagaa atagccagtt atttctgctt
    12661 ttttattttg tttttttata ggttatgatt tgtcaaatta agctagaata aaatgcttct
    12721 tgtacatagc tcaaaaatat ttcgattggt gttctgtaat tcaatgattt tcttggaata
    12781 tgattacgtt gattgctgac actgg
//
LOCUS       DAYYDS010000075        12788 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000075 DAYYDS010000000
VERSION     DAYYDS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.077"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACHA02_002160"
     CDS             133..3660
                     /locus_tag="ACHA02_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8670466.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACHA02_002161"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACHA02_002161"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8670467.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACHA02_002162"
     CDS             7448..7789
                     /locus_tag="ACHA02_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8670468.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACHA02_002163"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACHA02_002163"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8670469.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACHA02_002164"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACHA02_002164"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8670470.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHA02_002165"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHA02_002165"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8670471.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4327 a         2229 c         2717 g         3515 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaata
//
LOCUS       DAYYDS010000076        12734 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000076 DAYYDS010000000
VERSION     DAYYDS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.078"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHA02_002166"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHA02_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8670472.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACHA02_002167"
     CDS             complement(322..891)
                     /locus_tag="ACHA02_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8670473.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACHA02_002168"
     CDS             1077..1979
                     /locus_tag="ACHA02_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8670474.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHA02_002169"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHA02_002169"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8670475.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHA02_002170"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHA02_002170"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8670476.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHA02_002171"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHA02_002171"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8670477.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACHA02_002172"
     CDS             8547..8732
                     /locus_tag="ACHA02_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8670478.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACHA02_002173"
     CDS             complement(8794..9990)
                     /locus_tag="ACHA02_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8670479.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACHA02_002174"
     CDS             complement(10160..11596)
                     /locus_tag="ACHA02_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8670480.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACHA02_002175"
     CDS             11889..12698
                     /locus_tag="ACHA02_002175"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8670481.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAYYDS010000077        12621 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000077 DAYYDS010000000
VERSION     DAYYDS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.079"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1130
                     /locus_tag="ACHA02_002176"
     CDS             150..1130
                     /locus_tag="ACHA02_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8670482.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            1252..2670
                     /locus_tag="ACHA02_002177"
     CDS             1252..2670
                     /locus_tag="ACHA02_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8670483.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            2741..4210
                     /locus_tag="ACHA02_002178"
     CDS             2741..4210
                     /locus_tag="ACHA02_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8670484.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            4220..4687
                     /locus_tag="ACHA02_002179"
     CDS             4220..4687
                     /locus_tag="ACHA02_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8670485.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            4723..5595
                     /gene="scrK"
                     /locus_tag="ACHA02_002180"
     CDS             4723..5595
                     /gene="scrK"
                     /locus_tag="ACHA02_002180"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8670486.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            5676..8210
                     /locus_tag="ACHA02_002181"
     CDS             5676..8210
                     /locus_tag="ACHA02_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8670487.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            8366..9139
                     /locus_tag="ACHA02_002182"
     CDS             8366..9139
                     /locus_tag="ACHA02_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8670488.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            9132..9923
                     /locus_tag="ACHA02_002183"
     CDS             9132..9923
                     /locus_tag="ACHA02_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8670489.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            9933..10424
                     /locus_tag="ACHA02_002184"
     CDS             9933..10424
                     /locus_tag="ACHA02_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8670490.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            10442..10852
                     /locus_tag="ACHA02_002185"
     CDS             10442..10852
                     /locus_tag="ACHA02_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8670491.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            10856..12256
                     /locus_tag="ACHA02_002186"
     CDS             10856..12256
                     /locus_tag="ACHA02_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFM8670492.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            12279..>12621
                     /locus_tag="ACHA02_002187"
     CDS             12279..>12621
                     /locus_tag="ACHA02_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8670493.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNH"
BASE COUNT         4385 a         1698 c         2558 g         3980 t
ORIGIN      
        1 tatatcttta aacagctatt gccagataaa aataaatgat attatatata taaatactta
       61 tccgaaaagg ggaatgagag agtcaaggtt cttggaatgt cgaatagtcg agtaggtaga
      121 ttttctatct acaaccttgt tgtttaaaaa tgaaaaaaat tggaattaga gaagttgcga
      181 aagaggcaaa tgtttctcct acgactgtgt cgagggtatt gaatgatcgt ggatatttga
      241 gtgaggaaac aaaaagaaaa gtttttgatg ccatgaaaaa attagaatac tatcctaatg
      301 aactggcgag agcacttttt aaaaacgaaa catataccgt aggtttgatt tttcctacaa
      361 ttactaatcc atttcacgct gagttaattc aagatatcga gttcgagctt tccagcaggg
      421 gatataaagt gttgctgtgc aatagcttaa acaatccaac aaaagaaaaa gagtacttga
      481 cgatgttgag aaagaatcag gttgatggaa ttatagtagg aacgcacaat aaagatattg
      541 cggattatga tattccaggg ctacctattg tagcgattga tagaagtctt ggtgaaaata
      601 cagttacagt atcttgtgat aattacaggg gtggggaatt agctactcaa ctacttgtaa
      661 atcatggctg tcagaatata ctgtgtattc ggggagatag taagataaag ctacccgcga
      721 atagtaggac tttagcatat aaagatgttt tgaataaatt catgctggaa ccgttagtcc
      781 tagaagtacc atttgtaaaa gaaatcaatg aaaaaaatga aataattaag acatatctgg
      841 aaactcatcc aaatattgac ggggtttttg caggagatga tttattggct tctttggcga
      901 ttaacaactt aggaagttcg ggcaaaagag ttccaaaaga ggttaaagtg attggatttg
      961 atggagctca acaaacattg atttataatc cagaattatc aactattcaa cagcctatag
     1021 atcaaatatc taaagtagca gtaaaaaaat tattgaatat gattaagggc gaaaaggaga
     1081 cggatgaact ttcacttcca gtaaagctca taaaaaaaaa tacagcataa gttaaatcgg
     1141 cgaaaaattc cttcgtcgat ttaaaaatat ttttatgaaa cgggttgaca tatgaaacgg
     1201 gttgacatat attagttgtg aagataaaaa ccttttcact ggaggaagaa aatggagaaa
     1261 ttattaatta aaagcaataa tcatcaactt tactaccaac ctaaggatgt ttgggtcgga
     1321 gatgttatgc cttacgggaa agaaggaaaa ttctttattt atcatcaacg tgatactaga
     1381 aatccagttc cttttggaga accgtttggc tgggctttag caactacgaa agattttgtt
     1441 cattttgaag actttgggga atctctttta agaggggatg acaatgagag agaccagttt
     1501 atctacgctg gttctgtatt tgaggcaaat ggaaaggttc acgcatttta tacagggtat
     1561 aatcgtgaat tcttagcgca gggaaagaca tctcaagtgt tgcttcatgc tgagagtgat
     1621 gattttgttc attgggaaaa atcagaaaca gcattggagt tagaaccaca agaagggtat
     1681 gatattcgaa actggagaga tccatttgtc gtttgggatg atgaaaaaga agaatatttg
     1741 ctaattttag gggcgcgaaa gggtcaggat aaacatctac aaaccggacg tttagtggaa
     1801 tttacatcaa aagatttaac taattgggaa ttcaaaggtg atttttgggc tccgggattg
     1861 tataccatgt ttgaaatgcc agaacttttc aagattggtg actggtggta tcttgtctat
     1921 tcagaatact cagatggaaa taaaatactc tatcgaatga gtcaaagttt ggaaggtcct
     1981 tggattaagc ctaaagatga agcttttgat ggacgggctt attatgcagg tagaacagct
     2041 tatgatggaa aacgtcgagt cctatttgga tgggtaccaa caaaagatca agaaaatgat
     2101 aaaaacaatt atttatgggg aggtactttg gttcctcatg aagtctatca aaaagaggat
     2161 ttttcacttg gggtaaaacc catagacgaa gtgtggaatg cattcataaa tttacatgat
     2221 attgccgatg tagagataga aacagcagac acaagagaag aagtaacgct tgtcaaaaat
     2281 agtggaactt tgtttaattt agaaacaaca atttcatttg atgagaatct tagtgacttt
     2341 tcactgagat tatataaaaa ccttgagaca gatgaatctt acgaatatcg cttcaattta
     2401 gatgaggggc gtcttacatt tgataaaaac ccttgttacc cttggtttca atatatggat
     2461 aaaggactta ttagacctat atctttaaag cctaaccaat cttataaact aaaagtgatt
     2521 gttgatgaca atataatgac actttatatt gatggaatcg ctcttaacgc tcgtgtacat
     2581 ggttgttttg gtcaagcaat tactattaca gcaacaaatg gatcaatgac tgcaataaat
     2641 accaagtttt caaatgaaat aaagaaataa taaacgtcaa tcatgtggca ggtctgaaaa
     2701 tcaaacatga cctgtcacat ttaaaaggga ggataaatga atggactaca aaaaaattgc
     2761 cgaagatgta ttggaaaacg ttggaggaaa agagaatatc tcaagcgcta cacactgcat
     2821 tactcgttta cgtttaattc ttaaagacaa aagcaaagcg aatatggaaa tgcttgagga
     2881 aatagaaggt gcaaaaggtg ttcttttcaa tagtggacag ttacaagtaa ttttcggaac
     2941 aggtataatt gaaaaggttt acaaagagtt cgttgaattg acaggagcta aggaagtatc
     3001 cgtttctgaa atgaaagacg aaggagtcaa taattcagga aatagacttc aacgagcttt
     3061 taaagttttc tcggatatct ttattccgat cattccagct tttattgggg cagcaatggt
     3121 tttaggaatt cgttcgttga ttaccactcc tggaatgttt ggaatggatg gctcattcgc
     3181 tgatcacaat gtattcctaa atgatttatc caaatttctg ggtattattg caaccacatt
     3241 tgattattta ccagttttag ttatgtattc tgcaacaaaa cgttttggcg gaaaccctat
     3301 tttaggatta cttgtaggat ttgttatgat tcatccagga ctagccaatc ggaatgattt
     3361 cgtcatggga actataaagc ctgattattg gcatttattt ggattaagca ttccagcagt
     3421 tgcgtttcaa gggggtgttt ttccagcaat tttgacttct tggtttttag caaaagttga
     3481 gaaaattaca aataaatatt ctcctcaagt acttgcttac attttagtcc caactgtgac
     3541 aattgttctt gccaactttg cactattttt agtgtttggt ccattgggga attttatcgg
     3601 taatgtttta ggtggaatca ttgatttcct atatatgaag atgggtgcct ttggtgcttt
     3661 tgtttttgca ggcgcgttac aaccattagt tgtaacagga acacaacatg ctattcaagg
     3721 tattgaggca aacttagtcg caacaactgg attcaattat atccaaccta tttggtcagt
     3781 ttcaattatt gcccaaggtg gcggttgtat tggtatgtat ttactcttta agaaaaaatc
     3841 taaagataga gatattgcga tttcaagctt tattccaaca ctggtaggta ttactgaacc
     3901 ggctatcttt gctgttaatt tgaaatattc aatcgttcct tttgtttgct cgtttattgg
     3961 tgctggtttt ggtggagcat ttatgaaaat ttttgatgta aaagccattg gacaaggatt
     4021 gactgtattg ccaggtctga ctattgctaa tccagttctt ccttatatat ttggtaatct
     4081 catagcattc attttaccaa tcatttttat tctcgcttat gataaatata aagtgtttat
     4141 ccctgaagct aaacaccgta aatttgcaga aaaaactgac tcaaaaaaat tgccagttca
     4201 agtaaagtga gatgagagca tgttaaattt ttttagaaaa aatgaaataa gcgttgtagc
     4261 acctgcaacc ggtgtttttg aaaaactgga gaaattaagt gatccagtct tttcaaaagg
     4321 aatgatggga caaggatttg caattgatgc aaaaaacgaa acgattgtat ctccaatcga
     4381 tggttttgtt tcaagtattt ttcctacaaa acatgcctta ggaataaaaa caaaatcagg
     4441 aattgagata cttgttcatg taggaataga tacagttgag ctaaatggtg agggatttga
     4501 tattaaagtt caagaaggac aaaaagttaa ggctggggat attcttatga ccattaattt
     4561 tgtagttttt gaaaagaatc acaagactaa agatgtcatt gttgtttttc cagactacca
     4621 agacgaaatt ttcattccga aacttgaaga agtaaaacaa ggacaagaat tgtttgttct
     4681 tggctaatga taagtaatta ataatagaat cgaggaaaat gtatggtttt tggggctatt
     4741 gaagctggtg gaacaaaatt tgtttgtgcc attggagata aagatttgaa catatttgaa
     4801 aaagtcagct ttcccacgac tacacccaaa gaaacaatga atttagtttt agattttttt
     4861 aatcatcata aagatgattt agaaggtatc ggagtgggat cgttcggtcc tattgatatt
     4921 cattgtgaat ctaaaacgta tggcttcatt acatcaacgc caaaacttgc ttggcaggat
     4981 tttgatttta ttggaagttt aaaaaagcaa cttaatattc caactgcttg gacgactgat
     5041 gtaaatgcct cttgctatgg tgaatatgtt tctggaaatg gcagaggcct ttcaagtgtt
     5101 gtttactata ccgtaggaac gggcatagga ggtggcgcta tccaaaacgg aaacttcatt
     5161 gaaggtttta gtcatccaga aatgggacat atgcttgtaa aacgacacaa taaagacaac
     5221 ttttctggga catgtccatt ccacggagac tgtttagagg gcttagctgc tggtccggca
     5281 atcgaaagaa gatcagggaa aaagggacaa gaagtgactg aaaatgacgt tacttgggaa
     5341 atagaagctt attacattgc tcagtgtgca tataatacaa cattgatgtt atcacctgat
     5401 gtcattatat ttggtggcgg agtaatgaaa caaaaacact taaaaaagaa agtacaagat
     5461 aagtttattg agttgatcaa cggatatgtc gctattcccg atgttgacaa ttatattatg
     5521 acaccaaaat tgttagataa ttctggtatt gtaggttgtt tagctttagc aagagacgtt
     5581 aaagaaaaat cttaatttgg aaaaaagatc gtctcgacta tttgtttggt tgatgcgatc
     5641 tttttttcaa ttagtaattg aagggacgcc taaaagtgaa aaaaaaaatg aaagatgaag
     5701 tattagagta tatcaaaaat aatttaaagt attacgattt ttctgctcaa gatattgcaa
     5761 tgaaattttg tattaaaaga aatgtcgctt cccactattt gaatcagtta ttttcagatg
     5821 gaaaattact aaagaatgat tctgttagac cagtaatgtt caagtataat caacaaaagc
     5881 caaaagactg cttttctaag tttattggag cagacatatc tttgaaatcc actatagata
     5941 aatgcaaagc tactgtaatg tatcccccta atggcctccc attaataata aaaggaaaca
     6001 gtggcgttgg aaaaagtttt ttagcaagtt tgatctatca gtatgctctt gatagaaaag
     6061 taattcataa tgacgcaaaa tttgttgttg tgaattgtgc agattatgca aataatccag
     6121 agctattgtc agcagtttta tttggatata aaaaaggagc ctttactggg gctgaaaaag
     6181 atactgcagg gatgctatct tctgctaatg gaggatacct ttttttagat gaggttcaca
     6241 atctaagtgc agaaaaccaa gagaaattgt ttcttcttat ggattctcaa aagtatcgta
     6301 aacttggtga aagtgtgcaa tgggagtatg caaatgttcg ccttatctta gccacaacag
     6361 aagataaaaa tacctctttg ttagcaactt ttagaagacg catcccagct gaaatcactc
     6421 tgccagatta caataataga agtacatctg aaaaaattca attattattt gaatttctta
     6481 aagatgaagc aattacgatt gattcaaaaa tattttgttc gattgaactt tttactgatc
     6541 ttttatcgaa aacatttgaa ggaaatgttg gagaattgag gaatgaaatc aagcttcttt
     6601 gtgcagaagg ttatttagga aataagagaa agtcttcaat ttatttaggg gacaaactag
     6661 acagcggttt ttggatagat ccagaaatta gtatagattt aaaaaaaatg tttcttaaat
     6721 cactttctga aattgatttg attgaacttt ttcaaaatca catttcaatt gaagaaagca
     6781 tgagtcaatt aagagataga atacttaaag agtgtgcagg ttcttctgag tataattatt
     6841 tagaaagtga cgaatttata gcattaagaa actatgttgt taataaaata tcaccaatta
     6901 ttgattcgac aggtctatgt ttattagatg aattcttggc ggatatcagt ctatttataa
     6961 tgtttattga tgtaattgaa gaaacatcaa gaaatttttt gctgtctaag ttcagaaaat
     7021 ttcgtatgaa aaatgataaa caaaaattat tggcaaatga gatttggagt agtttagaag
     7081 tcgaagaaag taaacaagaa cttttgttga aatggatact ttttcttgta aagaagttct
     7141 atgtgaaaat accagaaacc cattgtctga tagttatgca cggtaaaata acagcttctg
     7201 cgatcgcaag ggaaacaaat aagttgctga atacatatgt ttatgaggct ttcgatatgc
     7261 caattgaagg tgagactagt gacttaatta acctaatcaa caatttttgt aaaagtatag
     7321 atacgacgaa tggactcatt ctgctagttg atatgggatc attggaacaa atgtatgaaa
     7381 aaattgaatc caatgtaata ggtgatttgg taattttaaa caatgtttct acagctttag
     7441 cgattgaatg tgggattcaa gtttgccaaa aaaagccaat tagtcatttt taccagatgg
     7501 attttgattc gtttcaagtt aaagttcagt attataaagg attatcacaa aagaaaaatg
     7561 taatagtatc ttgtctctct ggtgagggga tttctgaaaa agttaaggat attttaaaac
     7621 ggtatctcga taatcaagtg gagattttga tttttgattt taacgcatta aagaaaatag
     7681 caaaggaaaa agatactatg atttttaaga atacaatttg tgttctttca accactgaat
     7741 tttacattca aggaatcgat tgtttaaatt tagaaaatct aattaatggg aatcaaacct
     7801 tagaaaaatt aaataagtat atggactcgg atagctgtga gttctgctta aatgaattag
     7861 ttaaactctt tactattgaa ggcgctagta ctaagcttcg ctttttagat tccaaaaaag
     7921 tattcaatga gatagaaaaa gtactatatt tgtatgaaaa atattacgat gtcaaaatcc
     7981 caagttttct gagaattaat ctatttctac atctttctgg aatgattgaa cgaattatga
     8041 ttggtgatgg aataacaaat gaacatttga aagagggagc gcaactattt gatacttttt
     8101 taaatgtatc aaaagatttt ttttcagaaa tcaaagatat ttactgtatt gaaataccca
     8161 aaggagagta tgagttaatc tatacaattt ttgagcagac tattttctaa atagcatact
     8221 agcgtctaaa aaaatgaaag ggtgtccaaa gtatatttag acgctcattt aattaatatt
     8281 tactgtattt tcccgcttta ttggataatg ttagttggca cagtaattgc ttatataaaa
     8341 gtataagaag aaaggagaga acaaaatggc gttttggcaa acatttttag ttgtgttgta
     8401 tggatttttc attaactggg ataaggaagg gcccaaaatc ggaacctccc aacctgtcgt
     8461 tgcaggtttc ttgaccggac ttattatggg agatattcaa actggacttt atatcggtgg
     8521 gactttgcaa ttaatgacac taggtattac tagttttggt ggtgcatcag taccggatta
     8581 tcaaacagca gcattgatcg gtacttattt agcaatctca actaaccagg atgcttctat
     8641 aggaataact ttagcaatac cggtagcaat gttaattgta caactagatg ttctaaagtg
     8701 gtcaacaaac attttttttc aacaaaaggc agaaaaattc gcacaaaaag aagattatca
     8761 aaaattagag tggatgcaat atttggcatc aattaataca agctttgtat ctggaattcc
     8821 tgttttgtta actgtgatat taggcccaac gttagtggga agtgcaatca attatattcc
     8881 tgaatgggtt accggtggtt taaaggtagc agggggacta ttacctgctg ttgggatagg
     8941 aatgctactt aaatatttac ctacaaaaga ttactttgcc tatttgttag ttggtttcgt
     9001 tttggcagtt tatttccaaa taccaatttt agggatttcg ttacttggcg ctgcaatcgc
     9061 actaatcgta tataaaaata gatcaaataa tactcaagta gcaccagctg caacaggagg
     9121 ttttgacgaa gatgaataag aaactgacaa aaatcgacaa acaagtaatt ttccggtgga
     9181 ttctcaatgg tgcaacttca ttaaattatg aaaaaatgga agggttgggt tatgcgtatt
     9241 ctatgcttcc ttttatcaag gacacatata aagatgatcc tgaaggaatg aaacaatccg
     9301 ttttagctca cttacaattt tttaacacta cgccgtatac agcgccttac attattggta
     9361 tgaatgttgg aattgaaaaa gaagaaaaaa ataagtcttt ggaagcagta agtgctctga
     9421 aaacaggtct aatgggtcct ttagcgggaa ttggagatag tgtatttgta gttattccat
     9481 ggactatttt tggagcaatt gcagctaata tggcattgga aggtagtttt gttggaatta
     9541 ttttgtggat actggcatca attttattaa ggattactgc ttatccttta taccttgctg
     9601 ggaaagaatc gggtgcgagc ctactaactc aaattgaaac aaaattaaag tctattactg
     9661 atgcagtatc aattttaggg ttaatggtgg tcggtgcact cattgccaca gttgtaaaag
     9721 tgaatgttgg ctttgaattt aaacaaggcg acttaacaat gaccggaaat gaaatctttg
     9781 atcaagttat gcctggttta ttacctgcat gtgtagtagc aattgtgtat tatttgttag
     9841 gacgaaaggt taaaccagtt tatgtaattc tattaatctt agtagtttct attgcgttaa
     9901 gagcgttaaa tattttgaag taggtgttga atatggatga aaaaatgaaa ggtattatac
     9961 atgttcgtat tgatgatcga atgattcatg gacaagtagc aacacaatgg acaaatcaat
    10021 tgagtgctaa cagaattatg gttattaata atgaagtagc caatgatgac gtaaaaaaag
    10081 ctgtagttcg cctcgctgca ccacctaatg ttagaacttc aataattaca agggaagtag
    10141 cagttaaaaa tattctttct ggtaaatatg aaggacaaaa agttcttatt gttgcagttt
    10201 ctccaatcga tgttaaattt ttgattgata acggtttacc aataacaagt gtaaacgtag
    10261 gaaatttatc aagaaggaat ggaacagaac gcatccgtcc gacgataaat attactgaag
    10321 aagagagaga ggcttttaaa gaactaatta gtgatggagt tgaagtgaca gtgatacaaa
    10381 ctcctagaga ttcaaaagaa tttttaaagg attttattta ttaagaacag aggatgagtg
    10441 gatggagttt ataattacat ctcatggaaa tattgccatt ggatttgtag atacattaaa
    10501 aatgtttttt ggcgataatt taaattgtca tgtggtaaca cttggagatg caggcattga
    10561 agaattccgt gaaaacattg aaaaaatagt ttctcaagta aaaaatcaag aagttatggt
    10621 atttgctgac ttgattggtg gaacaccttt caacgaattt gctaaaagaa gtgaagtatt
    10681 tcaaaatggg tttcaacttc ttggtggcat gaatttagga attttgattg aggctgtcaa
    10741 tttgagatta caaggaaaaa aaagcgatga tatcatcgaa aatctaagaa atatgaatac
    10801 aattgcgtgt cttgcagata tgcaaagaca tactaacgaa gaggatgagt gataaatgca
    10861 aaaaattttt tacaagccta aagatttttg gtttggggat tgcatgcctt tttataaaga
    10921 tggaactttt tatctatttc atcaacgaga tacgagaaat cctaaacctt taactgatcc
    10981 ttttgggtgg tcactagtta caaccactga ttttgtgaaa tttgaagaat acggggaagt
    11041 aattcatcgt ggtggcaacg atgacattga tcaatacatt tacgctggaa gtattgtcca
    11101 aggaatcaat gagtatttag ctttgtatac tggacataat cgcaaagcta agttggatgg
    11161 aaaaacttca gaagtgttaa tgattgcaag ttctaaagat ttaattcatt ggaaaaagga
    11221 tggcgaagca gcaaaacttg ttcctcaaga aggatatgat aagaatgatt ggcgcgatcc
    11281 ttttgcaatt tttgatcaag atcatggaaa atacgttttg atactaggag ctagaaaacc
    11341 agcaagtaag aaaaagccga cggggagatt ggtctatttt gaatccgaag atttaaaaga
    11401 ttggaatttc aaaggagact actgggctaa taatgaattt aacatgattg aaatgcctga
    11461 tatatttgaa attgatggaa cgtggtattt acttttttca gagtattgtg aagataaaaa
    11521 gactaaatat cgagtcggag caggattatt ttcagattgg aaatcaccag tagacgaagc
    11581 tttcgacgga aaagcctatt atgcagcaag aacagtcgga actaatgaaa ctggccgttt
    11641 tctaactggc tgggtggcaa caaaagaagg ggagaacgat caaaataact gggattgggg
    11701 cggtgcactt gttccacatc aaattattca aaagacagac aaaactcttg gtgttaaact
    11761 gcctgaaaaa ataattgaga attttaaaga aatcactcca ttttcaagtt ttaagttaga
    11821 gaaagaattt ggaaaaaaag aaagagtaat tgttcctaaa atcgaagatt attttctatt
    11881 tgaaacaact ttgactatta catcgttaac aaaggagttt gctttaaaat tatttgtaga
    11941 tagtgatact caagaaggat acgaatttcg tatttcattg attaataatc agcttacttt
    12001 cggaaaaact ccgaactatc cttggcctca aatgtttgat aaggggctgc aacgaccact
    12061 atttttggaa ataggcaaag aatatgaact aaagttaatt gttgatgata cgatagctgt
    12121 tttatatatt gataatgttg ctttaagtac tagaatgtat gataaagtcg gaacagcatt
    12181 aagtatgact gttacggagg gagcaatcaa ggtagataaa ctagactatt caataaatta
    12241 tgaatgtgga ttttaaaaca attgaaggga tgatgtaagt gaaattaaat ggtttacaac
    12301 atattggaat tccaacaaaa gattttggag cttcaaaaat cttttatgaa tccttagggt
    12361 ttagtctgat taatgaagaa atgaatgtgg gaagtaaagt cggctttttc gagttgaatg
    12421 gaacgataat agaaatatgg gaagataaaa ctactgagtg tagaggagcc attgatcatc
    12481 tagcattaga tacggatgac atagatgagg tattcaattc aattcaagat ttaggatatg
    12541 aattattaga taaaaatatt atggaattac cgttctggga aaatggaatc cgttacttta
    12601 atttctacgg accaaatcat g
//
LOCUS       DAYYDS010000078        12205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000078 DAYYDS010000000
VERSION     DAYYDS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.080"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..216)
                     /locus_tag="ACHA02_002188"
     CDS             complement(37..216)
                     /locus_tag="ACHA02_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670494.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(232..432)
                     /locus_tag="ACHA02_002189"
     CDS             complement(232..432)
                     /locus_tag="ACHA02_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670495.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(597..1304)
                     /locus_tag="ACHA02_002190"
     CDS             complement(597..1304)
                     /locus_tag="ACHA02_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670496.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="ACHA02_002191"
     CDS             complement(1297..1734)
                     /locus_tag="ACHA02_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670497.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="ACHA02_002192"
     CDS             complement(1749..2000)
                     /locus_tag="ACHA02_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670498.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="ACHA02_002193"
     CDS             complement(2000..2206)
                     /locus_tag="ACHA02_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8670499.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="ACHA02_002194"
     CDS             complement(2643..2903)
                     /locus_tag="ACHA02_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670500.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3077..3181
                     /locus_tag="ACHA02_002195"
     CDS             3077..3181
                     /locus_tag="ACHA02_002195"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFM8670501.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACHA02_002196"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACHA02_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8670502.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="ACHA02_002197"
     CDS             complement(3810..4709)
                     /locus_tag="ACHA02_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8670503.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="ACHA02_002198"
     CDS             complement(4722..5315)
                     /locus_tag="ACHA02_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8670504.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="ACHA02_002199"
     CDS             complement(5391..5540)
                     /locus_tag="ACHA02_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670505.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="ACHA02_002200"
     CDS             complement(5556..7760)
                     /locus_tag="ACHA02_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8670506.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="ACHA02_002201"
     CDS             complement(7771..9231)
                     /locus_tag="ACHA02_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8670507.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="ACHA02_002202"
     CDS             complement(9292..11664)
                     /locus_tag="ACHA02_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8670508.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            12074..>12205
                     /locus_tag="ACHA02_002203"
     CDS             12074..>12205
                     /locus_tag="ACHA02_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8670509.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT         3040 a         2531 c         2053 g         4581 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccgt ttcctcctcg taaaatatca tttgtctaat
    11701 agtttttatt caaaatgttt ctagattata aaatttattg aataacacat tcctaacgga
    11761 atttgtttcg atattgtgtg atttctcaga gtacaatgga tgtatggaaa ggatccaacg
    11821 ggaatttgct atttcctcct gtagcgtttg tctaccctgt ttagtctgtt acccgaacca
    11881 ctgttatcac taccaactca ttagctgttt cgataatgga ctagactttc aaatagtagt
    11941 ttggaaggca gcttctagct cttttatcac cgtattctat taggcgaggt tgtggctata
    12001 cagtgagttt ctgggaaatc ccacagtatc gaagcatttt ccaaaattta tatcgaaagg
    12061 aggtccttct tccatgaaat tatttgtcgg tattgacgtt agctctgaaa aactagatgt
    12121 ttgtttttta acagacgaca atcaattgtc tatcttatct gaaatctctg ttgccaatga
    12181 catcgaaggt gcttcattca tacga
//
LOCUS       DAYYDS010000079        11709 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000079 DAYYDS010000000
VERSION     DAYYDS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.082"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACHA02_002204"
     CDS             complement(105..629)
                     /locus_tag="ACHA02_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8670510.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHA02_002205"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHA02_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8670511.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHA02_002206"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHA02_002206"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8670512.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACHA02_002207"
     CDS             complement(2351..3031)
                     /locus_tag="ACHA02_002207"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8670513.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACHA02_002208"
     CDS             3157..4026
                     /locus_tag="ACHA02_002208"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8670514.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACHA02_002209"
     CDS             4052..4567
                     /locus_tag="ACHA02_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8670515.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACHA02_002210"
     CDS             complement(4614..5177)
                     /locus_tag="ACHA02_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8670516.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACHA02_002211"
     CDS             complement(5232..5411)
                     /locus_tag="ACHA02_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670517.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACHA02_002212"
     CDS             complement(5488..5997)
                     /locus_tag="ACHA02_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670518.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACHA02_002213"
     CDS             complement(6093..6473)
                     /locus_tag="ACHA02_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8670519.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHA02_002214"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHA02_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8670520.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="ACHA02_002215"
     CDS             complement(7736..9013)
                     /locus_tag="ACHA02_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670521.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHA02_002216"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHA02_002216"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8670522.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="ACHA02_002217"
     CDS             complement(9935..10879)
                     /locus_tag="ACHA02_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8670523.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="ACHA02_002218"
     CDS             complement(10881..11567)
                     /locus_tag="ACHA02_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8670524.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3344 a         2474 c         2051 g         3840 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgt
//
LOCUS       DAYYDS010000080        11605 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000080 DAYYDS010000000
VERSION     DAYYDS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.083"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHA02_002219"
     CDS             6..305
                     /locus_tag="ACHA02_002219"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8670525.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHA02_002220"
     CDS             378..1739
                     /locus_tag="ACHA02_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8670526.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHA02_002221"
     CDS             1761..2021
                     /locus_tag="ACHA02_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670527.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHA02_002222"
     CDS             2035..2787
                     /locus_tag="ACHA02_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8670528.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHA02_002223"
     CDS             complement(2881..3486)
                     /locus_tag="ACHA02_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8670529.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHA02_002224"
     CDS             complement(3539..4513)
                     /locus_tag="ACHA02_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8670530.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHA02_002225"
     CDS             complement(4891..5610)
                     /locus_tag="ACHA02_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8670531.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA02_002226"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA02_002226"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8670532.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHA02_002227"
     CDS             7161..8558
                     /locus_tag="ACHA02_002227"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8670533.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHA02_002228"
     CDS             8639..9079
                     /locus_tag="ACHA02_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8670534.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHA02_002229"
     CDS             9227..9553
                     /locus_tag="ACHA02_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8670535.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHA02_002230"
     CDS             9554..10303
                     /locus_tag="ACHA02_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8670536.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHA02_002231"
     CDS             complement(10383..10838)
                     /locus_tag="ACHA02_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8670537.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHA02_002232"
     CDS             complement(11265..11540)
                     /locus_tag="ACHA02_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670538.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3664 a         2013 c         2357 g         3571 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaag
//
LOCUS       DAYYDS010000081        11584 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000081 DAYYDS010000000
VERSION     DAYYDS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.084"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..848
                     /locus_tag="ACHA02_002233"
     CDS             141..848
                     /locus_tag="ACHA02_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670539.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQALP
                     ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAI
                     AQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
     gene            complement(1010..2182)
                     /locus_tag="ACHA02_002234"
     CDS             complement(1010..2182)
                     /locus_tag="ACHA02_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8670540.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(2388..3203)
                     /locus_tag="ACHA02_002235"
     CDS             complement(2388..3203)
                     /locus_tag="ACHA02_002235"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8670541.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(3353..3799)
                     /locus_tag="ACHA02_002236"
     CDS             complement(3353..3799)
                     /locus_tag="ACHA02_002236"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8670542.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3796..4311)
                     /locus_tag="ACHA02_002237"
     CDS             complement(3796..4311)
                     /locus_tag="ACHA02_002237"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM8670543.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(4384..5298)
                     /locus_tag="ACHA02_002238"
                     /pseudo
     CDS             complement(4384..5298)
                     /locus_tag="ACHA02_002238"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(5295..7622)
                     /locus_tag="ACHA02_002239"
     CDS             complement(5295..7622)
                     /locus_tag="ACHA02_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM8670544.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            8231..8377
                     /locus_tag="ACHA02_002240"
                     /pseudo
     CDS             8231..8377
                     /locus_tag="ACHA02_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(8465..8758)
                     /locus_tag="ACHA02_002241"
     CDS             complement(8465..8758)
                     /locus_tag="ACHA02_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8670545.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            8795..8980
                     /locus_tag="ACHA02_002242"
     CDS             8795..8980
                     /locus_tag="ACHA02_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670546.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(9161..10339)
                     /locus_tag="ACHA02_002243"
     CDS             complement(9161..10339)
                     /locus_tag="ACHA02_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8670547.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(10443..10757)
                     /locus_tag="ACHA02_002244"
     CDS             complement(10443..10757)
                     /locus_tag="ACHA02_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670548.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            10864..11082
                     /locus_tag="ACHA02_002245"
     CDS             10864..11082
                     /locus_tag="ACHA02_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670549.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(11002..11472)
                     /locus_tag="ACHA02_002246"
     CDS             complement(11002..11472)
                     /locus_tag="ACHA02_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8670550.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGFPYFDFGWHIFHAKFCDFNSIGKI"
BASE COUNT         3353 a         2269 c         2024 g         3938 t
ORIGIN      
        1 aaaaaatccc gtgtatcaac acgggattag attaagtttc ctcaatcact attcataaca
       61 caattattat aacaagtaag taaacaattg aaaaggacat ttattgaaaa cggttgtttt
      121 ttacttaagg aggaaattta gtgaaaaaat tagttgcgtt gtttttggtc gcaggaagtt
      181 tgttgagtgg gtgtggtgct actacgtcta ctactcaaag cacaagttca gctcaggctt
      241 ccagcagcag taccacgatc tccgaaagta cggcagattc atcgaaagaa caaattgaaa
      301 agaaaaaaga agaagagaaa caaaaagcgg aaaaagctgc actagaaaaa aaagtgacag
      361 aagcggaatc tgctttgtca caagctgaag caaatcctac aaaagaaaac tatgcaactg
      421 cttcagcagc tatccaagcg ctacctgaaa acaaacaaga gttgacaagc cgtcttgctg
      481 caattgattc cacaatcaaa gcaaaagaag cagaagaaga acggaaaaag caagaagaaa
      541 ttgcacgaca acaagcagaa caagcgcaac aagaagcaat cgcacagcaa caagcagaac
      601 aggcgaggca agaagcaatc gcacagcaac aagcagaaca ggcgcgacag caagcagcag
      661 ctgaacaggc ttctcagcag gcacaagccg cagctcctga gcaaaataat gaacaaactg
      721 tctatgtcac accgaccggt tcaaaatacc atacacataa atgtggaaat ggtacgtata
      781 cccctgccac tttaagcgaa gctcaagctc gtggtttaac cccttgctcc aaatgttatg
      841 gaaactaatt caaaagttat actataataa taaaattctt ctgaaacata aaaaaataga
      901 agctggcgtt ccagatagta aaccaaaaat agcagtttac acgtccattt cgtcagcttc
      961 tctctttttt catttagtct gttccctctg cttttttgga catcttcatt cattctttct
     1021 caatcaaata gtacacctct ttttcttttc gcagtttata tcttcctttt ttcaatttca
     1081 ttgaaaactg tttcgaatgt ttatgtttta catggatttt aagcaggagc tcctcatgtt
     1141 gcaagcgata gttcctgcct tttaaatcag aaaatctcac tagtccgtat tcatcagaag
     1201 ctttcacaaa tggaactttt tctctagtaa tatagtgttt gccgttgagc gtttttacag
     1261 aaaagaccat tccttcgatt ggtcgtccgt ttgtgttctt tacataaaaa gataaaggat
     1321 agtttcgctt agcgatggcc aaagaatcta aataagcgat gagtagccaa actaagccga
     1381 ttgatccaag tagtataccg ataaaccaaa taaaaaattt ccattttttc cattgatcaa
     1441 ttccttggat cgcttttttt gcctcttttg cttgataagg aatacgatgc cccgtcacaa
     1501 gcagtcgatg tgtattgacc atataaggag tgcaggtaag caaagtcacg agatctctgc
     1561 ctttttggat acccaaagaa tctacttcgt caggtaagac cacttggatt ttttctactt
     1621 gatacgccaa tgtctttcca ttgatctgga tgaaaaactg gtctcctttt ttcaacttag
     1681 gcaaatctgt aaataatttt gcttctggga gtccacgatg cccagaaaga acggcatggg
     1741 tgtttttccc gccagtcgga taagaacttc cttccaataa tgacgttcct ttttgcaaaa
     1801 atatttccgt tgtctgatcg aatataggta agctgacatt gatttttggg atttcgatga
     1861 ctccaagcat atgttccata aaaaaggcat tactaggtaa atcttttaat gcttttgcat
     1921 caactgcttg gttgaaactg cctatccctg gtgatacgtt ttgctctgcc aactgttggt
     1981 ttttctttgt cattttttcc tgctgttttt ttatttcctc tgaattctta ttcgatgctt
     2041 gcttttgata atggatgatc atttgctggg caagaaaatc attcagtgaa tcttctacaa
     2101 aaggataaga aaagatcccg atcccaacga ctaaaatgat tgccatgaag atgtccagta
     2161 ttcttattct gcttttattc atcatgtcca cctattttct aatatgtata tgaagaggtt
     2221 gtgaaatcag cgccttgacc tgagtattag cgaagcaaac ggcaaaatag ttcctcctat
     2281 tttgccgttt gttgagcgaa taccaagaga ccgaccactt gtcacaacct ttctccccat
     2341 ttgaatcact atcgtttatt tatcgtatga tttttgtttc caatagttta agcttctgtt
     2401 tcttgatggc gttttttgaa atagatcact gctcctccaa ctaaagcagt acctaccaag
     2461 atgaatacga tgatccccat accaccagtt gaaggcaaga ttcctttagg ttggttatcg
     2521 atcgttgttt catcagaagt tgttggatca tcatctactg taaattcagt gtacgcagct
     2581 tcattcttag gtaatgcgta gccttctggt gcttttgttt cttgaaggca gtatgcccct
     2641 tgtttcagcc cttcgattga aaatttccct tctgaatcag aaatcaattt cacttcttcc
     2701 cagtcagaac ctgtgacttc atctgaccaa gcatatgggt attctcctga ggcaaggttt
     2761 gctatttttt tcattaattt aacagttgga tcttttgctc ttagaataga aaattctgca
     2821 tcggctaatt tttcttgcgt gttcacatct tgtttgatga agtggaaatc accgtagttt
     2881 tggacatttt ctacagtaat cgtgttatcc gtagttgtat tttcccgatt gactacaccg
     2941 tttggattcc caagattggc ttcatctgcg ccatggatga taccggttgt ttgttgcaat
     3001 tttccgtttt ttactataaa agtagtttct ttttgtggat tggttaattg ataaccatct
     3061 ggtgcttttg tttcaactaa agtataattg ccgtctatta atccattgac actgatggat
     3121 ccttcagtat cagttgtgaa tgtcaacgct gactcagcag gatcatagag agcgacacca
     3181 gtgttttcat caaaaccggt cacgaattga ttctctgaat ttttgatctg gaattgcgcc
     3241 cctgctaact tatctacccc tgaaaagttc ccagctttct ggattttgat attgctttct
     3301 ttgatgacat ttttagcata taggtgaatc gtgtctaaag gtttgtcagg gtctagggta
     3361 agcttaacac gatatttggc gacgtttcga caccgggctt tggtgtttct acaaagacgt
     3421 atacagcatc tttcccatca gatttacctg gaagagtcag gttagctaat ccttcaccag
     3481 aagtcgttac acttgcgaag ggtgtagcgt ctgctggaac tgctgtgtct ttacttggat
     3541 agtacgtagc agtgatgtca tatacgttga attcaacgcc gttcaacggt tcgctattcg
     3601 gaaatgtcaa ttccgttccg ttgtttattt gattttctgg tgctgaagtg aatttcaatt
     3661 tatgcaaatt aaccgtaaca ttttctggtg taggtgatac ggctgcagaa acctctgcag
     3721 ttcctaggcc gagaacgaaa ggaagcagca tgatagcagt tcctaaaaac gtccatattt
     3781 ttgttttgat gtttttcatt ttatttccct cctattgttt cctttttgtt gttttttata
     3841 aaaccaagct gtccctgttg aaaggactag caataaccct aaaattgaga aacctccaag
     3901 catctcttcg cttgtttttg gaaagcttcc tccatttttt tcatttgatg gaccatttgt
     3961 ctgcatctga actggcgttt ttccattatt cgtcggattt gcttttggtt cgttgatgat
     4021 ttccaatggt ataggatgaa gcggatcata actgcctttt tcaacttcaa agagaatggt
     4081 tgattgactc aatatatatc ctttaggcgg tttaatctcc actagttcgt aacttccaaa
     4141 agacaatcca tggacagaaa atgttccatc tttactggaa gtaaaaatcg ttgctcttga
     4201 atcatctttg gctacccaat gatttatccc gttttcttgc gctaagtatt ggtcattttt
     4261 tttgactaga aattgagcat ctgctagttt ttgtttcgtt tgccgatcca ctttgacaaa
     4321 ttgtttgcct cctgtttgga tggcaagatg tgtttctact gtttgattgt cagtcgtcaa
     4381 acgtacatcg ttatcaaatg tcgttttttc actgttttct ttcaatgtca tttggtaacg
     4441 gatcgttaat ttttttccag caaacgaact aagatctttt ccagaatctg ataagacaaa
     4501 accatgtttt attttttgtg tgtgaaatgt atgaatcttg tctccaccaa tcaaaaaatc
     4561 gatcgtttct ggttctagcc ataactgagg atcagctgta tctttgatcg tataggttcc
     4621 tatctcgttg atgttctcag gaattgttac ggtgatgaga taagggactt tatccccatc
     4681 tgcaaaatct gtctgcttat tctctactgt ttttgttaat gtgagtgcag caggtatttt
     4741 ttgttcgctc tttggataca agtggatggt cgtcaattta tgattagatg aatctgtcac
     4801 aggaagcaca acgacaaatg gtgctgattt ttcttttact taatctgaag tctttatttc
     4861 cgtaaatcgg taaacagcgt cacgaccttg ctcatctttg tccgatgcac taaaagaagc
     4921 aatgccctct tcctcgtcta ctgtttttgt cacattttct gccagtattt tttcactatc
     4981 acttttttgt gctagttttc tttgtgcgtc ttccacacta cttccttctg accgcagctt
     5041 gtaaaattca tccgagacat catatatagc gaaagtaacg ccgttcaacc cttttactcc
     5101 ttgaaagtta tccgggatat ttattccgtc gttttgactg atacttggca tcttatcgct
     5161 ttcgaacatt cgattatgca aagtaaatgt aataggtgat tctctagctg acacagtcgg
     5221 agaacctata aaaaagacca gtaggataaa aaagagaaca gcaaatatat ccacacgtac
     5281 attttttgtt tttttcatcg ctgttcacct gcttttttct tacgatggat aaaataaaag
     5341 aagccagaaa aaataaagat cagtcctaaa aagacaaagg gcagtagacc tatcccacct
     5401 gtcgaaggaa gcaagagagt cggattgatc gtcaacttag cggtatctgt tgcgataatc
     5461 gtataattat tgtttacact tccttcgcct aaactaatcg ttactatttg tgatccaata
     5521 tctgtccggc tgttaactgt tagggtataa tccaatgatt cgcctaagac aacaccggta
     5581 acgtggagat ttccaaccga aatcgagtga ctttgggcat cataattaaa ggcagcgtca
     5641 tcgacagtaa tcactgcgtt agctttatag atggtataag ttcctgatgt gttggctata
     5701 tcagtaaatg acaacttggg atttgctgct ttgactttgt ctattccgct attcgttagt
     5761 ttaattgtat agctgccagc attaaccata ttttttgctg attgtgcatc tgctgtgtaa
     5821 tccatactgc taagtgccac ttgtactgga gtaagcgaac tagtgacacc ctctgctagc
     5881 ttgacatgga ttgacgggac acctgctttt ccatcatatg tcttactgcc actgagctct
     5941 accgcaccgc tgatttgctt aatcgtatag acatcagatg tctgcacgat agtaaataaa
     6001 tagtttgttc tggcagctag tttagctagt ccgctttttg tcaattgcca agacgtatag
     6061 gttccaattt cagtaggcgc agttacttca actccctttt cattcatcgc tttgaaatca
     6121 gttggattcc atgtgatttg atcaatactg agatcattgt ttaaaaggga tgttgattct
     6181 gttttatcat taaaggcata tttaacgatc ggtacaacag aaagaccatc atagtactta
     6241 ctgccatttg cattgattga tactgttgca tttgtcttat aatataggat gacttcagaa
     6301 atctgttttc catttgcgtc ctttacgatt acatttccat ttgtatctaa actagctaaa
     6361 acactcattg ctgattggtt ctttgtttct atcgctgaaa cagctgtaaa ggtatatccg
     6421 ttgatagtag gcgtattagt cgtcaggtct tgtgcagcac ttccgtaagt aagattcatt
     6481 attttagaag gagcaatcat ctgtccaaaa tagtcttgat acttaatagt gattggtgtt
     6541 tgtttgcctg tataggtcac cgtatcgacg ttactttcta gggctgtgaa agaaccgttt
     6601 gtttgtgcaa aagaatagcc cgttactgct gttggagtga taaccacttt gtcacccaca
     6661 tggattccag ataatgtagt cccgtacgtt ccgatttttg ctgctccacc attgactgta
     6721 taagaaaaag ttcctgtccg attcgcattg acctgaaccg ttgctgcttg ataggttgga
     6781 aaatttgaaa catttccctt accatcaacg atctgcccat ttttgatcga gtaccccgtg
     6841 aatggaatat catttgctaa ctgtataccg attgaaaaag tactcatacc tcctccggca
     6901 ggaagccctc cccatgttga gggtgtaaaa gaataacttt tatccgcatt gggtgtaact
     6961 gaatcgatcg tatttccagc ggtagccacc cccatataga tactttttgg gaatgtgata
     7021 gtagtaccat ttactgtgga gccaataaac agattatgga tatctggacg gttatcaata
     7081 aaagcgattg ctttggttac gccttgtaac gcagttttat ccgcactcat cgatccgtta
     7141 accgcaacaa tgactccatc tttggcatat atcactgtat atccatctgg tatggtatgt
     7201 ccgttgaaat tcgcagtggc cgcaacagta ctaaatgtct ggcttctacc tgtttctgaa
     7261 taagctttct cccctgctgc ttttgctgct gtgatttctt tttctgtcgg atcaatcagt
     7321 tgagtcgagg atgtggttga ggttgtctcg ttgttgctag actgattggt gctttctatg
     7381 gactgtattt cagaataggg tggactattt gtctcctctg tcgtttggtt agcaaaattt
     7441 gacgtttctg tataaacgtt tacataaata cttgataagc tccctaagaa agtaaaaatc
     7501 aagatgatcg aaatcataat gggatgtaag tattttcttt tagatacagc tttgagtcta
     7561 gaaacaaaat gtttttgaca tatacgttgg attttatttt gagttttctt aatcattttc
     7621 atccctcctc tcaataaaaa atcatcaaat aatcctttac acgttattct aaatttaatt
     7681 tttattattt tattgatgta tagaaagttt ggggcttttt ttctcttttt attctaaggt
     7741 tattttagtc ttgtccttta atacaaagat tttattttta aataaaaaat actctttttt
     7801 tacgattatt gaaaaaaaaa gataaaactt gattaattga agtattgctt aaatttggaa
     7861 gctatgaggg aagaataaaa gagttaggac gggattatct actatttttc tttgttttaa
     7921 atatttgtta tctttgaaca taaaagagaa atgtgtcgac tgcagtgttg tgtccctcat
     7981 cacgaataaa cggtgttcaa ttagctgatc ttgtattcac ttaaaaaatg gtaaagtacg
     8041 agttgctatt ccccattttc aattttctaa cgctcaggac aaaatactgt tttcacaacc
     8101 tcattggaat aaaagcatat atattgtttc aagaacgaaa tcttgttatc tttttaaagg
     8161 gcatctgtcc aaaatctaat tttaggaggt acgttttggt atttattcgt acaaataaaa
     8221 gagaattatc tttattcatg taacgcatcc aattgatttt ttcatgtacg ctcctggaac
     8281 gattttactg acgattgctt atttaactgc gaatcgctca ttagctttta tcatagctat
     8341 ccatattttg aataacgttc ttggctttgt attatagata cagatgacaa aaaaagttca
     8401 agtatgcggt gtagaaccaa tatcgcatac ttgaactttt ttcaatgagt ataaattaaa
     8461 acttctaatt ttcaattccc atcatctgat atgcccgacc gtctgaactt cttttgaaaa
     8521 agccaaatgt gattagataa cgtctttgtt cgacataatc gccactagaa aatacgttca
     8581 gcagtatcga attaacctct gtttctgtat aagtcactcc atggtcaaag cgtttcaaga
     8641 cttctttcaa taggatgtat ttcttttttg ttgaacttgg tacttgtttt acttttccat
     8701 cattcatgaa tgtgtctaaa actttcatct tttcttgctc cgtataagta taaaccatct
     8761 tctttctcct tttgattcta agaatactcc atttatgtta gaatattctg gattttttat
     8821 taaagcggtt actcttaaga aagatattat catagtgctc ttttttttac tactacttat
     8881 aggtagattt attctaatcc attattctcc cctaccaata attaaagaaa aaactggatc
     8941 agggtgttca aattacatgc taagaaaaaa gaacccttga ataccaaggg ttctttctca
     9001 gtctgataac aatgattaac gacggatttc tttgatactt gtgaaaagtc gttaaagtat
     9061 tgatataaaa ggatttttat tacctctgac tacatattga ctaatttgtt aaaaaatcgt
     9121 ttttcaagaa tcctgtaata ccagtgcttt caccgttttt ttaactctct aaagaactaa
     9181 aagcaatcag aggttttacc ttttcatttg acgttaagtg gcgataaatt ttcgaagttg
     9241 tttggtcagt atggcctaag cgatctttca agacttccca gctacagtgt tccgctacta
     9301 agcttgccat tgtatgacga gctaaatgag tatgaaatgg cttattaatt cctgagcgat
     9361 tcacgcaccg tttgacgtga tcatttacat actctcgacg ataaggaaca ccacgtttac
     9421 tagaatcaag atacacaaac aaatatgttt cttctataat aaacctgtaa tgttgcctca
     9481 tctcacccaa agccttaatc aactgttgta gcttttccat aactagagga gttactagaa
     9541 ctcgtctagc agaactaact gtcttagtag tttcaattcg aaaatcctta ctcttacctt
     9601 taactgccgt ttgcttatag aacagaattt catttctttt gaaatctata tctccaggct
     9661 gtagagcaag cgcctcgctg actcggcagc cagtgtaaaa tagaacatgg aaaagtacag
     9721 acatcggtaa ttcctcatga acaatgccat aattcaaaaa agttctcaat tcatcgacag
     9781 taagaaactt gtcctcaata ttatttatgg cttcttttaa atcagttacc gttaactgat
     9841 attttggcat tctcacagac ttcattggat tttcacttat caaattcttt ctttgacaat
     9901 atttaaaaat catatttaga acttgaataa gtgattgtaa actatttttg gcatagccaa
     9961 catttgttcc attaacgcca cattccttta aggtaaaaag caattcttga aggcactctg
    10021 gtgtcaaatc agacaacttt ttatcaccaa tccatcgaac aataattttt aattgttcct
    10081 ctcgcttatc ataggttatt tccttttgtt catttaacga acggtaatat tggaaccatt
    10141 gctcacctac aacactaaac ttgtcattgc ttgcaacaat ttttttctta agcctttgtt
    10201 gtttctccat ttccagataa gcgagttgcg cctcacgttt tgaattaaag ccacgtcttg
    10261 ttgttcgata tgcttgaaag taccatgtga tcctaccagt agaactgatt tgcttcttga
    10321 atcgatctgt tttttccatt tattgattcc tcctattttg aaggaaccca acaagcagaa
    10381 accctttgga aatctttcta tctatgtcat cattgtaccg catcttagaa agaaaatgta
    10441 acttataaag atcgaagtag tcgcaatttt tctcgatagt cattcgaatc actctttctc
    10501 atccattcta aaacatcatc acgaaaatag tgtcctcgaa tcccgtacca acagggagga
    10561 aactcaccaa gttttactaa acggttcagt gtactatcac ttatctgaaa tagtcgcttt
    10621 aaatcagtat ttgttaatac cagaggtaaa tgactgactg caagcaattt ttccgtgtaa
    10681 tcttccagtt ttttttgtaa aattgaatag aactctattt ccagagaatt aacatttgtt
    10741 ttaacttcca actccatcac ccttctctct caaaatcaaa acgaccacca cggtacccct
    10801 taaaattgta aaaaaacact ccagtactcc atcaaaacgg actagaaaag aggaattttt
    10861 aatatgaaac aaggaattgc agacataaaa ataattaaag aaattcttga aaaatctacg
    10921 gctaatgcaa tagcattcgg aacaggaatc aacttgagta cagtaaaaaa acttaaaagt
    10981 ggagagagag ctgaagaaaa attaaatctt gccgatgcta ttaaaatcac agaatttggc
    11041 atgaaaaata tgccaaccaa aatcgaaata tggaaatcct agctcttcca aagccatttg
    11101 tcgttggtac caaacactgc cgagactttt cccaataata tcgccaattt cacgatcatt
    11161 aagaccgatg aagaaggaca taaaaatgat ttcttgtctt tgactaggta atgctgcaat
    11221 cgcatttgcc aactcctcat tggaaataaa gattttcata ccaaggacaa taaaagtacc
    11281 actactattc aaatttggtt ccaaataata gaactctgta cctaaccctt tttgttccaa
    11341 tatttccatt gatatttgcc gttggcgaaa tctagcgtac ttcttttgaa tatttaacgc
    11401 ctcattgcag actactttct tacaaaaact gtcaaacgta tgttgaatgt gggtttcctc
    11461 tgagtcgccc ataatattct ccccctcaaa aaattcattc aactcgtttt acttctatcc
    11521 ctttccatta ttactacaat ctaggaggta agcgccccat agaacagtac ctgtacgtca
    11581 aata
//
LOCUS       DAYYDS010000082        11378 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000082 DAYYDS010000000
VERSION     DAYYDS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.085"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..525)
                     /locus_tag="ACHA02_002247"
     CDS             complement(<1..525)
                     /locus_tag="ACHA02_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8670551.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFEST"
     gene            complement(537..1229)
                     /locus_tag="ACHA02_002248"
     CDS             complement(537..1229)
                     /locus_tag="ACHA02_002248"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8670552.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1248..1532)
                     /gene="macP"
                     /locus_tag="ACHA02_002249"
     CDS             complement(1248..1532)
                     /gene="macP"
                     /locus_tag="ACHA02_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8670553.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1545..2108)
                     /locus_tag="ACHA02_002250"
     CDS             complement(1545..2108)
                     /locus_tag="ACHA02_002250"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8670554.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2336..2998
                     /locus_tag="ACHA02_002251"
     CDS             2336..2998
                     /locus_tag="ACHA02_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8670555.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3122..4318
                     /locus_tag="ACHA02_002252"
     CDS             3122..4318
                     /locus_tag="ACHA02_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8670556.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4419..5795)
                     /locus_tag="ACHA02_002253"
     CDS             complement(4419..5795)
                     /locus_tag="ACHA02_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8670557.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5832..6698)
                     /locus_tag="ACHA02_002254"
     CDS             complement(5832..6698)
                     /locus_tag="ACHA02_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8670558.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6787..7812)
                     /locus_tag="ACHA02_002255"
     CDS             complement(6787..7812)
                     /locus_tag="ACHA02_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8670559.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7830..8504)
                     /locus_tag="ACHA02_002256"
     CDS             complement(7830..8504)
                     /locus_tag="ACHA02_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8670560.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8688..9581)
                     /locus_tag="ACHA02_002257"
     CDS             complement(8688..9581)
                     /locus_tag="ACHA02_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8670561.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9655..11352)
                     /locus_tag="ACHA02_002258"
     CDS             complement(9655..11352)
                     /locus_tag="ACHA02_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8670562.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLAKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3373 a         2507 c         1959 g         3539 t
ORIGIN      
        1 tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacggc
       61 atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac
      121 acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg
      181 gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt
      241 ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc
      301 cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg
      361 atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg
      421 agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc
      481 tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat
      541 acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata
      601 aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc
      661 aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa
      721 gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat
      781 gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta
      841 accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt
      901 cctggtttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata
      961 acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc
     1021 atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct
     1081 ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg
     1141 cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct
     1201 tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg
     1261 attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa
     1321 gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc
     1381 tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt
     1441 tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt
     1501 tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc
     1561 aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc
     1621 atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct
     1681 tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca
     1741 tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag
     1801 ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga
     1861 tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg
     1921 acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccacc aggatgaaat
     1981 accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca
     2041 ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg
     2101 ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat
     2161 ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc tctaaatata
     2221 gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa taagtatgta
     2281 atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat
     2341 aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa
     2401 tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact
     2461 ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact
     2521 tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac
     2581 aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa
     2641 acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga
     2701 attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc
     2761 gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa
     2821 acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat
     2881 cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc
     2941 tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact
     3001 cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt
     3061 tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt
     3121 tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga
     3181 tttattgaac aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga
     3241 gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga
     3301 acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt
     3361 catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa
     3421 tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg
     3481 tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt
     3541 cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc
     3601 acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa
     3661 tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta
     3721 tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat
     3781 gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca
     3841 attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat
     3901 ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat
     3961 ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac
     4021 cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga
     4081 cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa
     4141 tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac
     4201 aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt
     4261 agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat
     4321 cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg
     4381 cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt catcttgctg
     4441 tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg
     4501 atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat ggagacgatt
     4561 ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt
     4621 ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt
     4681 agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg
     4741 gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga
     4801 agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc
     4861 gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt
     4921 cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg
     4981 attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca
     5041 atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg
     5101 gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa
     5161 ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata
     5221 aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc
     5281 ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca
     5341 atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct
     5401 aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct
     5461 agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca
     5521 acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa
     5581 cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga
     5641 ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca
     5701 ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga
     5761 ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg
     5821 gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa aatgactaaa
     5881 aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa
     5941 atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa
     6001 ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa taatccacag
     6061 atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg
     6121 cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag
     6181 ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc
     6241 ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca
     6301 gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc
     6361 gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa
     6421 ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca
     6481 ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg
     6541 attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt
     6601 cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc
     6661 catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac
     6721 atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac
     6781 gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt
     6841 cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt
     6901 tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt
     6961 aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt
     7021 aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc
     7081 cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc
     7141 atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa
     7201 ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat
     7261 gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc
     7321 atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc
     7381 gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga
     7441 gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg
     7501 ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc
     7561 actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct
     7621 tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc
     7681 taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat
     7741 cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt
     7801 aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt
     7861 gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg
     7921 actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt
     7981 tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt
     8041 ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta
     8101 atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt
     8161 aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct
     8221 gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg
     8281 atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga
     8341 agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga
     8401 ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat
     8461 tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc
     8521 tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa
     8581 aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct
     8641 cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt
     8701 ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc
     8761 actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt
     8821 tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg
     8881 tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta
     8941 attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg
     9001 agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc
     9061 gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct
     9121 cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca
     9181 ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc
     9241 atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa
     9301 ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc
     9361 ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt
     9421 tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga
     9481 tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga
     9541 ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt
     9601 aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac
     9661 ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc
     9721 gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat
     9781 attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata
     9841 gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc
     9901 ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa
     9961 gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct
    10021 atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc
    10081 agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc
    10141 atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc
    10201 ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc
    10261 atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg
    10321 tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat
    10381 cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat
    10441 cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt
    10501 atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa
    10561 ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata
    10621 agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc
    10681 ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc
    10741 tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat
    10801 catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata
    10861 agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac
    10921 ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg
    10981 atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga
    11041 attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac
    11101 ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt
    11161 agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa
    11221 tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttggcta gttttccgtt
    11281 cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat
    11341 ttttttattc atttttttct ccccgttctt tttttatt
//
LOCUS       DAYYDS010000083        10922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000083 DAYYDS010000000
VERSION     DAYYDS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.086"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACHA02_002259"
     CDS             complement(<1..281)
                     /locus_tag="ACHA02_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8670563.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            453..1541
                     /locus_tag="ACHA02_002260"
     CDS             453..1541
                     /locus_tag="ACHA02_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8670564.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="ACHA02_002261"
     CDS             1669..3045
                     /locus_tag="ACHA02_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8670565.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="ACHA02_002262"
     CDS             3446..5026
                     /locus_tag="ACHA02_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8670566.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="ACHA02_002263"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="ACHA02_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="ACHA02_002264"
     CDS             complement(5652..5972)
                     /locus_tag="ACHA02_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8670567.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="ACHA02_002265"
     CDS             complement(6120..8354)
                     /locus_tag="ACHA02_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8670568.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="ACHA02_002266"
     CDS             8833..9099
                     /locus_tag="ACHA02_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8670569.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="ACHA02_002267"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="ACHA02_002267"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8670570.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aa
//
LOCUS       DAYYDS010000084        10807 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000084 DAYYDS010000000
VERSION     DAYYDS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.087"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..162)
                     /locus_tag="ACHA02_002268"
     CDS             complement(25..162)
                     /locus_tag="ACHA02_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8670571.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACHA02_002269"
     CDS             complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACHA02_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8670572.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="ACHA02_002270"
     CDS             complement(1599..1853)
                     /locus_tag="ACHA02_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670573.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="ACHA02_002271"
     CDS             complement(1878..3020)
                     /locus_tag="ACHA02_002271"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFM8670574.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="ACHA02_002272"
     CDS             complement(3080..4432)
                     /locus_tag="ACHA02_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8670575.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="ACHA02_002273"
     CDS             complement(4503..4847)
                     /locus_tag="ACHA02_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8670576.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="ACHA02_002274"
     CDS             complement(4857..5231)
                     /locus_tag="ACHA02_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8670577.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="ACHA02_002275"
     CDS             complement(5244..5558)
                     /locus_tag="ACHA02_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8670578.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACHA02_002276"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACHA02_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="ACHA02_002277"
     CDS             complement(8976..9638)
                     /locus_tag="ACHA02_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670579.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="ACHA02_002278"
     CDS             10255..10485
                     /locus_tag="ACHA02_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670580.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="ACHA02_002279"
     CDS             10491..10790
                     /locus_tag="ACHA02_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8670581.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         2997 a         2384 c         1832 g         3594 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaac
//
LOCUS       DAYYDS010000085        10723 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000085 DAYYDS010000000
VERSION     DAYYDS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.088"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(241..1089)
                     /locus_tag="ACHA02_002280"
     CDS             complement(241..1089)
                     /locus_tag="ACHA02_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8670582.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1253..2155
                     /gene="gnd"
                     /locus_tag="ACHA02_002281"
     CDS             1253..2155
                     /gene="gnd"
                     /locus_tag="ACHA02_002281"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8670583.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2157..3158
                     /locus_tag="ACHA02_002282"
     CDS             2157..3158
                     /locus_tag="ACHA02_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8670584.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3155..3439
                     /locus_tag="ACHA02_002283"
     CDS             3155..3439
                     /locus_tag="ACHA02_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8670585.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3472..4269
                     /locus_tag="ACHA02_002284"
     CDS             3472..4269
                     /locus_tag="ACHA02_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8670586.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4283..5116
                     /locus_tag="ACHA02_002285"
     CDS             4283..5116
                     /locus_tag="ACHA02_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8670587.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5134..5991
                     /locus_tag="ACHA02_002286"
     CDS             5134..5991
                     /locus_tag="ACHA02_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8670588.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6013..6522
                     /locus_tag="ACHA02_002287"
     CDS             6013..6522
                     /locus_tag="ACHA02_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8670589.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6559..6960
                     /locus_tag="ACHA02_002288"
     CDS             6559..6960
                     /locus_tag="ACHA02_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670590.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7072..7425
                     /locus_tag="ACHA02_002289"
     CDS             7072..7425
                     /locus_tag="ACHA02_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670591.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7459..7902
                     /locus_tag="ACHA02_002290"
     CDS             7459..7902
                     /locus_tag="ACHA02_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8670592.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7919..9004
                     /gene="uxuA"
                     /locus_tag="ACHA02_002291"
     CDS             7919..9004
                     /gene="uxuA"
                     /locus_tag="ACHA02_002291"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8670593.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8997..9710
                     /locus_tag="ACHA02_002292"
     CDS             8997..9710
                     /locus_tag="ACHA02_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8670594.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9917..10066)
                     /locus_tag="ACHA02_002293"
     CDS             complement(9917..10066)
                     /locus_tag="ACHA02_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670595.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10462..10662
                     /locus_tag="ACHA02_002294"
     CDS             10462..10662
                     /locus_tag="ACHA02_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8670596.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3588 a         1694 c         2320 g         3121 t
ORIGIN      
        1 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
       61 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      121 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      181 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      241 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      301 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      361 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      421 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      481 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      541 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      601 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
      661 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
      721 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
      781 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
      841 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
      901 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
      961 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1021 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1081 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
     1141 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
     1201 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
     1261 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
     1321 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
     1381 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
     1441 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
     1501 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
     1561 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
     1621 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
     1681 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
     1741 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
     1801 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
     1861 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
     1921 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
     1981 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
     2041 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
     2101 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
     2161 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
     2221 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
     2281 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
     2341 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
     2401 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
     2461 tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
     2521 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
     2581 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
     2641 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
     2701 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
     2761 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
     2821 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
     2881 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
     2941 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
     3001 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
     3061 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
     3121 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
     3181 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
     3241 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
     3301 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
     3361 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
     3421 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
     3481 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
     3541 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
     3601 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
     3661 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
     3721 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
     3781 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
     3841 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
     3901 gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat gcatgacgct
     3961 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
     4021 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
     4081 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
     4141 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
     4201 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
     4261 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
     4321 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
     4381 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
     4441 gatgattgtt acagccgctt tgcaaatgat ttatatgggg ggtttttctc caggaggatc
     4501 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
     4561 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
     4621 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
     4681 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
     4741 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
     4801 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
     4861 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
     4921 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
     4981 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
     5041 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
     5101 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
     5161 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
     5221 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
     5281 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
     5341 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
     5401 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
     5461 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
     5521 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
     5581 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
     5641 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaatg
     5701 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
     5761 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
     5821 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
     5881 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
     5941 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
     6001 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
     6061 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
     6121 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
     6181 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
     6241 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
     6301 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
     6361 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
     6421 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
     6481 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
     6541 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
     6601 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
     6661 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
     6721 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
     6781 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
     6841 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
     6901 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
     6961 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
     7021 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
     7081 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
     7141 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
     7201 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
     7261 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
     7321 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
     7381 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
     7441 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
     7501 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
     7561 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
     7621 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
     7681 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
     7741 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
     7801 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
     7861 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
     7921 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
     7981 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
     8041 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
     8101 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
     8161 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
     8221 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
     8281 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
     8341 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
     8401 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
     8461 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
     8521 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
     8581 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
     8641 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
     8701 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
     8761 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
     8821 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
     8881 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
     8941 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
     9001 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
     9061 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
     9121 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
     9181 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
     9241 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
     9301 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
     9361 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
     9421 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
     9481 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
     9541 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
     9601 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
     9661 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
     9721 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
     9781 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
     9841 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
     9901 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
     9961 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    10021 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    10081 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    10141 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    10201 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    10261 cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa
    10321 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    10381 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    10441 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    10501 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    10561 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    10621 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    10681 cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa att
//
LOCUS       DAYYDS010000086        10192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000086 DAYYDS010000000
VERSION     DAYYDS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.089"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..626
                     /locus_tag="ACHA02_002295"
     CDS             120..626
                     /locus_tag="ACHA02_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8670597.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="ACHA02_002296"
     CDS             643..1542
                     /locus_tag="ACHA02_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8670598.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="ACHA02_002297"
     CDS             1629..2513
                     /locus_tag="ACHA02_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8670599.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="ACHA02_002298"
     tRNA            complement(2705..2776)
                     /locus_tag="ACHA02_002298"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="ACHA02_002299"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="ACHA02_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8670600.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="ACHA02_002300"
     CDS             4319..5410
                     /locus_tag="ACHA02_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8670601.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="ACHA02_002301"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="ACHA02_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8670602.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /gene="pgk"
                     /locus_tag="ACHA02_002302"
     CDS             6562..7752
                     /gene="pgk"
                     /locus_tag="ACHA02_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8670603.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /gene="tpiA"
                     /locus_tag="ACHA02_002303"
     CDS             7856..8611
                     /gene="tpiA"
                     /locus_tag="ACHA02_002303"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8670604.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="ACHA02_002304"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="ACHA02_002304"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8670605.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2111 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct ca
//
LOCUS       DAYYDS010000087        10086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000087 DAYYDS010000000
VERSION     DAYYDS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.090"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..759)
                     /locus_tag="ACHA02_002305"
     CDS             complement(247..759)
                     /locus_tag="ACHA02_002305"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8670606.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACHA02_002306"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACHA02_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8670607.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ACHA02_002307"
     CDS             complement(3212..3655)
                     /locus_tag="ACHA02_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8670608.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ACHA02_002308"
     CDS             complement(3656..4444)
                     /locus_tag="ACHA02_002308"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8670609.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACHA02_002309"
     CDS             complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACHA02_002309"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8670610.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ACHA02_002310"
     CDS             complement(5559..6146)
                     /locus_tag="ACHA02_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8670611.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACHA02_002311"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACHA02_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8670612.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ACHA02_002312"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ACHA02_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8670613.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ACHA02_002313"
     CDS             complement(9063..9998)
                     /locus_tag="ACHA02_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8670614.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DAYYDS010000088        10056 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000088 DAYYDS010000000
VERSION     DAYYDS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.091"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(520..744)
                     /locus_tag="ACHA02_002314"
     CDS             complement(520..744)
                     /locus_tag="ACHA02_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670615.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            935..3712
                     /locus_tag="ACHA02_002315"
     CDS             935..3712
                     /locus_tag="ACHA02_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8670616.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3793..4212
                     /locus_tag="ACHA02_002316"
     CDS             3793..4212
                     /locus_tag="ACHA02_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8670617.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4224..4694
                     /locus_tag="ACHA02_002317"
     CDS             4224..4694
                     /locus_tag="ACHA02_002317"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8670618.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4711..5490
                     /locus_tag="ACHA02_002318"
     CDS             4711..5490
                     /locus_tag="ACHA02_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8670619.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5477..6298
                     /locus_tag="ACHA02_002319"
     CDS             5477..6298
                     /locus_tag="ACHA02_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8670620.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6315..7364
                     /locus_tag="ACHA02_002320"
     CDS             6315..7364
                     /locus_tag="ACHA02_002320"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8670621.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7377..8381
                     /locus_tag="ACHA02_002321"
     CDS             7377..8381
                     /locus_tag="ACHA02_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8670622.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9014..10033
                     /locus_tag="ACHA02_002322"
     CDS             9014..10033
                     /locus_tag="ACHA02_002322"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8670623.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3372 a         1655 c         2097 g         2932 t
ORIGIN      
        1 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atctttttgc
       61 tacgtaattt ttgtaaaaaa gtgggttgag agaaatctaa ttgattttca aagaaggagt
      121 atgaaaatga gaaaaatatt tattcgttta ttgtgggtac tatcttagag gaataaataa
      181 tagtggagaa aactatggtt tggcgcagat tctatcaggt ttgatggaac gaagacaacc
      241 tcccgacatc aagtcaaaag ctacttgaga ttttgatctg aaaataaaaa aagaaaacaa
      301 ggtaggaaca tcaatgtttc taccttgttt tcgtaatcaa tgggaaaaaa ctaagagtgg
      361 tgtacgtaat caataaaaag gatggtgaca acagtacctc tttactctct gaaagcatca
      421 taactcgttt ttcctcatta tgccaataaa ttgttgttat accacatata aggatttgta
      481 atagctaaaa acagacagga aagttgaaat ctgtgaatct tattttgtat ggaaggattt
      541 atagaacgca aaaatcaaga taaagatgaa taaaaatcct gtttcggcta tatattgcgt
      601 atctacgcca ataaaacgag tttgttctgt catagccata taaagcaaag gcaaaaatac
      661 aactacagcg agataagatc cgatgatttc tcgaaaagtt ttccaggaag tatgtttcct
      721 gtttttatat ttatccgatt tcattgaata gtcatagcct ccttattctt tcatatgttg
      781 aaattccagt ataaaaactg gaaggaactg tacgtttttt agaataaaaa ttataaggga
      841 taagtaggct gctgtctata ataagatata tttttcaatt ctttattaaa gattttattg
      901 acaaaatgaa tccgttttct atgatggagg aggggtgaat aagttgttga agaaggaaat
      961 tttatgttac ttagagaacc agacagcctt tttcgattta gaacatatga acgaggtctt
     1021 tacagcacaa aggttagctg atcattttgg ggtaaaaaga aacacagtca gtcattattt
     1081 gaaccagtta aatgatgagc aactactagt caaaatcaat tctcgaccag tgatttattt
     1141 ccacaaagaa gcttttgaaa aacaattttt taagttacgc acgaattttt atttttctat
     1201 cgatgatctc aaagaggaac agcctttttt cgcgcaacca aaagacttat tctcttttat
     1261 gatcggatac gacgccagtt tgaaagaaag tatcgaacaa atcaaaacag ctctttacta
     1321 tccagatggc ggattaccac ttttgattac aggcgaaagt ggcacaggta aaagttattt
     1381 agttaatttg gtctatcagt attgtctgct tcatgatctg ctagaagatt ctgcaccatt
     1441 catcactttt aattgtgcac aatatgcaga taatcctgaa cttttgacta gcaatttgtt
     1501 tggacatgtt aagggagcgt acactggtgc agaagaaaat aaaaaagggg cttttgaagc
     1561 agcagatggt gggatcttgt ttttagacga ggtacatcgc ttaacaccag aaggtcagga
     1621 aaaactgttc acctatcttg atcaaggtgt gatttatcgt atgggagaac ctaatttgtc
     1681 acgccggatc aaaacacgtc tcttttttgc tacgactgaa gagattacta gtcatttctt
     1741 aacaactttc attcgaagaa tacctatccg aatcgaacta cctgcactta atcagcgtag
     1801 tcgtaatgaa cggctagaac tgatttactc tttttttctc gaagaacaaa gaaaaatggg
     1861 gagccctttg tcagtcagtg gaccagcttt gtcactttta acaagtcaga aattatccgg
     1921 aaacatagga gagctgaaaa atatcgtcaa agtttcagca gcaaaagcat atgctgaaca
     1981 gcgtgaacag cctgaaatcc atgtcacgat ccatcatttg caaaaagaac tacttgcgca
     2041 gcctattact aaaaatacag cacagcaagg aatctatatt acgcaagacc agaccttaga
     2101 gcaattaatg gagaagcaac agcccgaaca gcagcgaatc gttaaaagtt ttgagcaaat
     2161 cctaattgat ttcaagaaaa ataactgtct gctatattct tgcgaaggaa aattaaagca
     2221 agaagtgatt caattatttg attttttgct gtttgagacc agtagacaag aaaaacacga
     2281 gctgctggtt tattggacac aatctatccg agagaccttt cgacaaatgg aaactgccta
     2341 tcaaatcaaa ttcgatggaa acagtgtcta tgccttgagt tactatctct atcagcgaag
     2401 cactatcaaa tggttaccgg aagaacatga tattatccaa ctaatcaaag aattagaaca
     2461 acaagtttct cagtcttatc catctagtta tcactatgtc caacgaattc tggaattaag
     2521 taagatcaaa ttagatattg aagacacaag tatggatcgg attttactga cgatctatct
     2581 aaaaaaagcg aaatggtcaa aagaaaaacg actgccaaaa gcgatgattg ctgctcacgg
     2641 ttatgcaact gctagcagta tggcaaatgt agtaaatcgt ttattacaag aggatctatt
     2701 tgaatcattt gatatgccac tagatgtcac accacagcag attgcttcag aaatccttga
     2761 ttattgcgaa aacagcgatg tttcaaatgg attgatcata ttagtagaca tgggatctct
     2821 gaaagaaatc catcagtttt ttaaaaaaca gttatccgta ccgcttttga ttttgaataa
     2881 tgtgacgaca cctttagcaa tcacagtagg ggaatgcctg caaaaaaatg tatcattaga
     2941 agagatagca gaagagactg ttcgacagat ccagccagaa tggcgactgc tctatccaga
     3001 ggaaaacaaa cccaaagcac tcattactac ttgcttcact ggaatcggta cggcggtcca
     3061 cttaagtgaa ctgctagaaa aaagcttacc tacggcttgt cagttgaaaa tcatcccata
     3121 tgaatatcag caattaaaag ataagaaaaa cagtgaccca ttattttcca tatacgaagt
     3181 cgtaggaatg attggcacca cagatcctga tatcaccaat atcgactatc tttcattaga
     3241 agacctcatc tcgggggaaa agatgtctgt actagctgaa tggttggaca ctactatgaa
     3301 tgcttcagag aaggaaatat tcaatcagcg catcattcgt aatttttcat tggaaaaagt
     3361 actggattcc gtgactattc tagatacaga gaaggtgatg ggagaaatcg atcattttat
     3421 gagagaatta gaggtgcagt tgaatcgaag catcagcaat cccaaaaaat tagcgttgta
     3481 tgtccatgtg agctgtctca ttgaacgatt gatccgacaa atgccaatcg aaacctatca
     3541 aggattagag aaatggaagc agtgtcaaaa agaagggtta tctgctatta aatctgcgtt
     3601 tagtgtcatt gaagagcatt atagtgtcat gatccccaat tctgagattg cctatatcta
     3661 tgatattttg agtgaagata cagagtttct ctcgatagaa gaagaatttt gaacataagc
     3721 aagttgttgc cactaatttt ggcatgcaac ttgctttttt agtatgtgta agtgaaaaga
     3781 ggagatgaaa aagtggatag agaaattatc ttagcttccc atggaaaatt tgcttcaggg
     3841 atattagatt cattagagct gatctatggg aaaaatcatt ctattactgt attcgattgc
     3901 tatactgatg agaattttga tttagcggaa acagtaaggc agctttttat gaggtacaaa
     3961 gataaagaaa tcattgttct gacggatctt tttggcggaa gtgtcaacaa cgaattctta
     4021 caatatatca atcgggatca tgtctatctc gttgccggat tgaatttgcc actattgatt
     4081 gagctagtat ctcggttgga cgctgaagta gagacaactg ttttgatcca gcaggtgcta
     4141 actgattcaa aaaaaatgat ccagttttgt aacgagcggc tcaatcagaa aatagaagaa
     4201 gacgaatttt aggaggaaga aaaatgattg tattgacaag agtcgaccat cggttattac
     4261 acggacaagt agctttttca tggacacaaa caataggcgc agactgtatt ttgattgcta
     4321 atgatgatgt acctacgaat gagatacgaa agacaacgat caaattagcc aagccacaag
     4381 gagtcaaact agtgatcaaa tcaattgatg actcgattgc cgcgttgaaa agcggggtga
     4441 cagataaata taagttattc atcgtagtgg aatcgatcga ggatgcatat aagctggcag
     4501 aagcgtatcc agaaatcaaa accatcaact taggagggat caaggcaaaa cctggtactc
     4561 gcagcatcgg aaaagcggta aatattttac cagaagagga ggaacgcttg aaacgattaa
     4621 ttgacaaaca aatagaagta gaaatccgtc aagtaccagc agataaaaaa gtggatgtaa
     4681 gagaagtttt gtaagaaagg aagaacctca atgattgtac aagcagtctt attaggaatt
     4741 gttgcatttg tcgcacaatc agaatatgca ctagggacat cgttgctttc tcgtccgatt
     4801 gtgacggggc tgtttacagg aatcgtgtta ggtgatgtaa aaactgggat cattatgggc
     4861 gcaactttag agttagcatt tatcggatcg ttctcagtag gtgcttcgat tccacctgat
     4921 gttgtgaccg gtgggatttt agggacggct tttgctatta cagctggagc cggtacagaa
     4981 accgcgcttt tactaggttt gccgattgct acgttgacat tgatattgaa aaatatttac
     5041 ttagggcttt tgattccaat tatgaaccat aaagcagaca attatgcaga agaaggaaat
     5101 tataaaggag tcgagcatat gcacttgctt gccggtatcg gtctttcctt gatgcttggt
     5161 gttgtggtaa tggtttctta tatggtggga agcaatacga tcggaaatct attaaatatg
     5221 attcctgact tcgttcaaaa ggggttgtca gtagctacag ggttgattcc agctcttggt
     5281 tttgcgatgc ttgctcgttt gctgatcaac aaacaagttg caccatattt tttcttaggt
     5341 tttgctatag cttcttactt agagattcct gtgaccggag tagctatttt tggtgcgatt
     5401 ttagcagttg tggtagtcaa tattatgaat gtccgtcaac taccacttca aacaactgca
     5461 ggggaggtag aagaagatga agaattctga gatcaaaaac agcaaaataa cgaaaaaaga
     5521 gctaaatcac gtattttggc gttcttttca aatggagttt tcttggaatt atgaacgtca
     5581 gatgaatatg gcttatgttt atgcaatgat tccgattttg aaaaagttat atcagtcgaa
     5641 agaagaaatg gcagcagctt tgaaacggca tctagagttt tttaacacga ccccacatat
     5701 cgttacactg attttaggaa tcacggctgc aatggaggaa gaaaataagg atgatcctga
     5761 atttgatgtg actgcgatcg atagtatcaa aacatctttg atgggacctc ttgcagggtt
     5821 aggagattct ttcttttggg gaactttgcg attgattgcc acaggtgtgg gtacctcttt
     5881 agccatgcaa ggaaatattt taggccctat cttgttcatt ttgattttca atattccgca
     5941 cattctgttt cgctatctag caacaggttg gggatatcgc ttaggaacgg gttttttgaa
     6001 aaagatccag gcaaacggta tgatggaaag tttgacatta ggcgcgtcta ttattggact
     6061 gatggtcgtt ggagcaatga ctgccacgat gatcaccatc aatattcctt taaaaatcgg
     6121 ttcaggagaa aatgctgtga ctgttcaaag tatttttgat gatatcgttc caaatatctt
     6181 atgtcttgga gcatttggca tagtgtttta tctattgaag aaagaagtga aaccattaac
     6241 gatcttgatc ggactagctt tctttggaat ttttggttcg tggataggga ttttttaaaa
     6301 aaggagcaga gaaaatgttt acgatgcaag attatatcta tgaagaaaag gaagtactaa
     6361 gtacgatctt gaaaaagaat gatttttcga caagagaaca tatgaaaaaa acagtgaatt
     6421 tactgatctt agcaacgggt tcatcttata acgcttgttt agcagcgaag ccagcattgg
     6481 aatcttatgg tgatcttact gtcgatattc aagaaccttt ttattttttc aattatggaa
     6541 aactttctcc ttcaatcgat actgtgattg ctgtgtcaca aagcgggaaa agtgcttcta
     6601 ctgttgaagc agtaaaaatg atacaaaaac aagggttacc tgttgttgct atcacaaatg
     6661 acgtgcaaag tccacttgct ttagaagctg cacaaattat tgatttaggt gccggggtag
     6721 aatcagtagg cttcgttaca aaaggctatt ctgcgactgt tttacagtta ctactcttag
     6781 ggataggaat tggaataagt aaaaataaaa tcagcaaaaa aatagaacaa gactacatgc
     6841 aacagttgcg caaaataatc aaccatttac cagcgattat tcaaaaaacg gaagaatttt
     6901 ttgatggata tcaaagttta tttcgtttag cacagcgttt cgtcaccatt ggatatggtc
     6961 caaactgggg aacagctaaa gaagcagaga cgaaattgac tgaaacaatc cgcgttccgt
     7021 cacaaggatt tgagttagaa gcttatatgc acggcccata cctggaggca gatgcttcgc
     7081 atttgctatt ctttattgaa ggggaaagtg taaataagga acgttcgcaa aagttgcaaa
     7141 gatatatgag tcggtacgtt ggagagactc tcaccattac cacaaaaaaa gcaagaaacg
     7201 agaaaacact tggattagca atagaatgtg atgaatatct gtctgtatta gcgcttgtcg
     7261 ttccgtttca actattcgct tataaaaccg cagtagctaa aggtattgac ttgaacaaaa
     7321 agatttttga agactttgat acagtcttga aaagtaaatt ataagaggtg gaaatcatgc
     7381 tgaaattcaa tgaagaagaa caaatcaaag caataaaagg cgcactagct ttacgtccgc
     7441 aagtagaaga aatcatcgat aaactttatg cggaaaaatt tgacgctgtc tattatctag
     7501 gaatcggtgg aacgtatgct tcctcgatgc aagctgtcac ttatatgaat gggaaaagca
     7561 acttgcctgt ctttgtacag catgctgcag aatattacac tactggaaac aaaagattaa
     7621 caaaagattc tatcgttatt ttgtcctctg ttaccggtac aactcaagaa gtagtgaaag
     7681 cagtagaaca aatcaagaaa gtaggagcga cactgattgg atttatcgat aaagcgaaca
     7741 gtaagttgtc tcagctatgc gattttgtgg tcacttatcc agctcctgga acagaacaaa
     7801 tcaaattttt tatggcagca gatcgattga tgtatttgaa tggagaattc gaagcttatt
     7861 cagaatacta tgaacagctc gaacgttatt taccaacagg gttagtggaa gcagagaaaa
     7921 aagcagatgc atttggctta tcatttgcag aaaaacatcg acgtgatagt atgcattatt
     7981 ttattggagc agggaatcaa tggggagctg tttattcata tgccatgtgt tactgggaag
     8041 aacaaagctg gcttccatcg aaatctatcc atgctgccga gtttcttcac gggacgttag
     8101 agatcgtaga agaaacaacc ccagtcacat tatttttagg cgaagatgaa cagagagtgt
     8161 tatctgaacg tgtggctaaa cttttgccaa aaatttgcag caattataca ttgatagaca
     8221 caaaagacta tccagtagaa ggtatcagtg aaaaatatcg cggacgtgtt ttgtctttct
     8281 tattgatgca tgtagtgaca caacgcatcg atgctcatgt ggagcagttg aactgccatc
     8341 cattagagat acgccgctac tatcgtcagt ttgattatta ggatagactg gataaaaaga
     8401 atataaaaaa taaagagagc agcgataact gctcccttta tttttttaaa atattggcat
     8461 caattacgtt ataaaacaca ttttttgcat tatctacagt tcaacttata atctgtttaa
     8521 tgggacatgt gggtgccaac cattttttgt tatgcaatag tactatttag ttgttgcatg
     8581 gatccgtagt aaaagtccaa gtacaacgag ttgcgccagt ttacatagcg ttttgtatcc
     8641 agaatgttga ctaaaacaaa agttcctatg tcaaccgtca acagagacaa tctgtccgtt
     8701 gcaggacctg acagatttag aaatgttcaa tctgtctctt atgtatttta aatcaatact
     8761 cactaggaga agtttcctaa cctcctaata gtaatgctgg tagtaacatt gtcaaaactg
     8821 cttcctctta ggaacaagca aagcataacg agtgtaaaaa taaaatgtag actattgtaa
     8881 catatcattg attatttcaa gtaaaaacga taaaagaagt aaacaaacac ttaaaaacaa
     8941 tggaaattag tcccaacttt aagaaatgta tactataatg gaaattatca attatttatt
     9001 tggaggaata tccatgaaac aaaaaatcgc agttcttggt cctggatctt ggggcactgc
     9061 tttagcacaa acattggcag aaaacgggca tgatgtccgt atttggggaa atgtacctga
     9121 acagattgat gaaatcaata agtatcatac aaatcagcat tttttacctg atttgacgat
     9181 cccagaatcg attatcggat ataaagaatt ggcagaagca gtggataacg cagatgctat
     9241 tttatttgtt gttccaacaa aagcgattcg ttcagtcgca caggaactta ttggtaaaat
     9301 gaatactaag ccaatcatta tacatgcaag taaaggacta gaacaagata cgcataaacg
     9361 aatctcagaa gttttggctg aagaaattcc agaagaaaaa agacaggcaa ttgttgtatt
     9421 atctggtcct agccatgctg aggaagtggc agtacatgat atcacaacaa ttacagctgc
     9481 aagtattgat caaaaagcag cagcctatgt acaaaaactg ttcatgaatg attatttcag
     9541 gatttataca aataatgatg tgattggcgt agaaactggt gcagcactga aaaatatcat
     9601 tgcaattggt gccggcgcta tccatggatt aggattcgga gatgatgcaa aagcagcaat
     9661 catgactcga ggattggcag aaatcagccg tcttggtgtc gcgatgggtg ccaatcctct
     9721 gacatttatc ggtttgagtg gtgtggggga cttgatcgtt acttgtacaa gtgttcattc
     9781 tcgcaattgg cgtgccggta acttgttagg acagggacat aagctggaag aagttctaga
     9841 aaacatggga atggtcgttg agggtgtggc aacgacaaaa gcagcagtag agctagctca
     9901 acagctaggt gttgagatgc caattactca aacaatctat aatgttttgt ataatggaga
     9961 agatatcaaa aaagcagcaa aagaaatcat gttacgtgac ggaaaaatgg agaatgaatt
    10021 ttctcttcgt taaatagatg atcaatagtt aatcgt
//
LOCUS       DAYYDS010000089        10020 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000089 DAYYDS010000000
VERSION     DAYYDS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.092"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..892
                     /locus_tag="ACHA02_002323"
     CDS             113..892
                     /locus_tag="ACHA02_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670624.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            938..1882
                     /locus_tag="ACHA02_002324"
     CDS             938..1882
                     /locus_tag="ACHA02_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8670625.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1898..2677
                     /locus_tag="ACHA02_002325"
     CDS             1898..2677
                     /locus_tag="ACHA02_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8670626.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2689..3387
                     /locus_tag="ACHA02_002326"
     CDS             2689..3387
                     /locus_tag="ACHA02_002326"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8670627.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3415..4179
                     /locus_tag="ACHA02_002327"
     CDS             3415..4179
                     /locus_tag="ACHA02_002327"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8670628.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4223..5674
                     /locus_tag="ACHA02_002328"
     CDS             4223..5674
                     /locus_tag="ACHA02_002328"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8670629.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5706..6842
                     /locus_tag="ACHA02_002329"
     CDS             5706..6842
                     /locus_tag="ACHA02_002329"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8670630.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            6872..7948
                     /locus_tag="ACHA02_002330"
     CDS             6872..7948
                     /locus_tag="ACHA02_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8670631.1"
                     /translation="MHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKSDYDEEIISLG
                     GKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKAAKKAGIPIRI
                     AHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKFTLIPNAVDAK
                     KFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFHAIKNNSVLLI
                     IGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLFEGVPLVGIEA
                     QFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSERSILIKAD
                     YNIQTAYKILESKYYELFELIQRG"
     gene            7954..8976
                     /locus_tag="ACHA02_002331"
     CDS             7954..8976
                     /locus_tag="ACHA02_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFM8670632.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            8998..>10020
                     /locus_tag="ACHA02_002332"
     CDS             8998..>10020
                     /locus_tag="ACHA02_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8670633.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKN"
BASE COUNT         3659 a         1422 c         2075 g         2864 t
ORIGIN      
        1 tatttctgtg aatatattag tttaattatg tttttttaaa taaaaagctg tcatttttat
       61 gtaagtgaat tatatcttaa tttatctatc atcaatactt aggagtgaat ctatggaaaa
      121 gccattcgtc aagtcattga ctgctgtggc ggtaggtgca ggtgctattg cgatctgttt
      181 gtttggatac catatcaata atcagcggca acatcagcaa cggatcaatt atgcagaatc
      241 tgccatcacc aatcaaaaag acaccttgac ttctttatca aaagaagtag acaagctgta
      301 tagtacaaaa gaaaaaattt ttctaaaccc tgaaataaca gaagaaacaa tcacaagttt
      361 gtcaaataag ctatcctctg tcaaactttc agcagatgat tacgacatca aagaatcaga
      421 attgcctaaa gaagctgttg caatccaaga tgagaagaaa gcaatcgtga cacagttaga
      481 tagtgcagaa tcaaaactga agatccagac agctgtcaac gatttattta ctaaaaatgt
      541 atcaaactgg cagcaagctg tagacgatgt catcatcaaa gaaaaggtga caagtgcaga
      601 tgtcgctcgt gtacgtgaaa atatgagctt ttttaaagat agtccttgga aaacggttgt
      661 tatgcaatat ctaggattcg ctgatattca gatcgcgcaa gtcacgcaac tagatcagtt
      721 attcgatacg atgttaaagg atggtcaagt gactgccacc gctacttacg atcaatattt
      781 gacggtgctc agtcaaatag agcagatccg gaatgaaaaa ataagcgcag catacgcaac
      841 aaaagcacaa actgttgctg agcaaatggg ctatagcaat ataagctatt gacagttaaa
      901 acgttgattc aagcaaggtt agcaaacgga ggaaactatg gcaagacgaa gaagaaaatc
      961 aagagggaaa aaaatatttc tgattatttt agcagttctt atcgtattag tattagcaat
     1021 tgtaggagta ggagcaaaat tatatctgga tctctcacat tctattgaga aaacttacga
     1081 atcagtggaa cgaacccaag gagaacgaac gaaaccagtt gatctggaca aacaagaatc
     1141 tttctctgtt ctattgatgg ggattgatac tggagattta ggtcgggtcg atcaaggaag
     1201 atcagatacg atgatggttg ccacagtcag tcctgaagac aaacagacaa cgatcgtcag
     1261 cattcctcgt gatacgtatg tcgacatcgt aggtcgtgac acaaaagaca agatcaacca
     1321 cgcttacgca ttcggcggag ctgccatggc gatggatacc gtacaaaaat atgtagacat
     1381 cccaatcgat cactatgtct ctatcaatat gaaagggcta aaggaactcg ttgatgcagt
     1441 tggtggtatc gaagtcaata atgacctcac tttctcacaa gatggctatg actttccaat
     1501 cggtaaaatt acattgaacg gggatcaggc attatcttat tctcgtatgc gttatgaaga
     1561 cccaaatggt gattacggta gacaagggcg ccaaagaaag atcgtagaag gcgtagcgaa
     1621 aaaagtattg agtctaaacg gtgtcagcaa ctatcaatcc gttttgaatg ctctagagtc
     1681 gaatatgaaa acagacatga actttgatga aatgaaaaaa attgctttag attatcggga
     1741 ggcatttggt acgattaagc aagatcagct gcaaggagaa ggcttcatgc aagataatat
     1801 ctcctatcaa aaagtcagtg atcaagaact tgatcgtgtg aaaaaagaat taaaagatca
     1861 aatgaatcta gagaataaat aggaaagtgg agaaaaaatg gaagagacaa tcagcttgaa
     1921 agaaattttt gaaacattac gcaaacactt ggtaataatc atcatctcaa tgtttgctgg
     1981 cttggccatc tcaggaatcg taacattttt catcttgaca ccaaaataca gttcacaagc
     2041 acaattgatc gtgacactgc cacaatcaga cactgcaaat gtcaatgatg tcaatacgaa
     2101 cctgcaaatg atcaatacgt ataaagatat gatcgtcagt gatttagtgc tcaatgaagt
     2161 caaagatcgt ttagaaacag aagacaatgt gaaaatgact gctgaacaaa tcaaagatgc
     2221 catctcagtt aatcaggcag aaaattcaca aatgttctcg attcaggcaa taagtacgaa
     2281 tgcagtggct gctcaacaga tcgctaacac aacttcacaa gttttccaag agaacgcaaa
     2341 agatgtgatg aatgttgata aaatctcgat catttcaggt gcagttgcca atacgacacc
     2401 tgtttcgcca aataataaat tgaatttaat gactggctta atgttaggaa tgatggtagg
     2461 tgtaggtctg gcattcttat tggaattact cgatcggaca gtgaaagatg aaaaatatgt
     2521 gacagataca ttaggattcc caatcttagg aacagttcca gaaatgtcag cgaaagaatt
     2581 gaatgctggt atccgtaaaa aaggaccagc tcagacacaa ccaaaagcta caaacgataa
     2641 aacagcgcca tcacgcagaa gccgctcaag agtctaggag gaaagaaaat ggcacgaaca
     2701 cagaaacaaa aagcagccca aactcatgca gtcagtttgg tcacactcac caatccctct
     2761 tcaccaatcg ctgaacaata tcgtacgatt cggacgaata tccagtttgc ctccgcagct
     2821 ggtcagcaaa tcaaaacgat tgttgtgact tcatcaggac ctggagaagg gaaatcgacc
     2881 actgcagcaa acattgccgt tgtttttgcc aaatctggtc aacgggtgct gctagtcgat
     2941 gcagacttac gtaagcccgt catctataaa acgttcaagt tgaacaacgc aagcggatta
     3001 agtactgttc ttagttcatc aggaagtgta gcagatgaga tccaacgaac accagtcgac
     3061 aatttgtcga ttctgccaag tggacctaaa ccaccaaatc catcagaact actaagttcg
     3121 ccaagaatgg atcagatttt agcagaagca agacaactat ttgatgtagt gatcttcgac
     3181 atgccgccag tcgttgccgt aacagatgca cagatcatgt cttcaaaaac ggatggcacc
     3241 ttattggtcg tacgggaaaa tacgtcacga aaagaatcat tgactaaagc aaaagaactg
     3301 ttagatatgg tacaagcacg agtcttaggt gtcgtataca acggagcaga acacagcaaa
     3361 gatgctggtt attactacgg aaattaagga ataagaagag aggagacaag agaaatgatc
     3421 gatctacatt gccatatcct accaggaatc gatgacgggg cagaaaactt agaagcaagt
     3481 attgcaatgg ctgaaaaagc gattcagcaa ggcattacgc atattttatg cacaccacac
     3541 cacaataatg gaaaatacag caatgaaaaa tcacaagtca tttctctagt cgcttcgtta
     3601 caagccgaat tagagaaaag acagttgcct ttgacgttat tggaaggaca agaagtacgt
     3661 attaccggaa cgctcatcga agacatccac cgagatgaaa ttttatttac agatttagat
     3721 gatacatatc ttctgatcga atttccgaca ctggaagtgc cgttatatgc agaacggctt
     3781 ttttttgagt tgttagaact aggaaagata ccagtgatcg tccatccaga acgcaacgcg
     3841 cattttcgag aagacccaaa tcatttgctt ccgttcctag atatgggatg tttggcccaa
     3901 cttactgcgc caagttatgt tggtgtattt ggcaaagata tccagaaaac agccaaaaca
     3961 atggtcaaac ataatctcgt gcagatggct gcttctgatg cacacggggt ctctaagcgc
     4021 accttttact taaaagagtg ctatgagcag attgccaagg agtttggtaa ggaaaaagta
     4081 gtacagatga aacaagtcgc aaaggatctg atcaacgggg atgagatcaa ttatccaatc
     4141 tatgaagagg taaaaaagaa gaagtttggt ttattttagt tctttattcg tagataaaaa
     4201 ggaaatgagg agagagtgag agatgtatag aaaaaactca agtggttggt ctaaacatat
     4261 tgattttatt ttattagact tattttgtgt ccagcttgct ttttatatat catatgtaat
     4321 gcgacagggt gactggaatc cgtatgtgat tcctatttat cgccacatgg caatttttat
     4381 agaacttgag aatcttacga ttatattttt atttgaggca tataaaaatg ttttaaaaag
     4441 aggatattat aaagaggctg tagcttctat aaagcaatct tttatgctgt tacttgtgtg
     4501 cagtttatac cttattacaa tgcaggatgg aaatgattat tccagagttt ctttatttat
     4561 aatgggggtg gtgtatggat tattaacata tataattcgt atactttgga aagggttgtt
     4621 gcatcaccgc atgaaaaacg gtgaagatgt atcgttagtt attgtaacat ctcaaaagat
     4681 tgcaaaaaat gtagttcgta atcttaaaga gaagaattat ggcatgtata aacttagtgg
     4741 tgtgattgta gtagacagag atatgtatgg aactaccatc ggaggtgtcc cagtagttgc
     4801 aaatgctgat aatgcagcgg aatatctttt acagaattgg gtagatgaag tattcatcaa
     4861 tgttgacgaa agtgttccat atcccaaaga attaatcgcc agatgttcgg aaatgggact
     4921 tgctgtacat atgaatctta caaaagtgtc tgatagtaca cgtggaaaac aatttattgg
     4981 ccgagttggt gattatactg ttattacatc tagtattaat gttatgacga tgaggcaagc
     5041 atttataaag agaacgattg acattcttgc tggaattatt ggatgtattg caacaggtgt
     5101 tatttttatc ttcgtagcac cagttattta catcaaatca ccagggccga ttttctttgc
     5161 acaagaacgt attggtcaaa atggtaagac atttaagatg tataagttcc gtagtatgta
     5221 tatggatgca gaagaacgca aagcagagtt gatgaaagaa aataagatga gtaataatct
     5281 catgtttaag atggattttg acccaagaat cattggcaat aaaatacttc ctgatggaac
     5341 aaagaagaca ggaattggac aatttattcg atctacatca ttggatgagt ttccacaatt
     5401 ttataatgtc ctaatgggaa aaatgagcct ggtggggttt agaccttgct tgaagtcaga
     5461 atatgagaag tatgattttc atcatcgtgc gagaattgcg atgaaacctg ggattactgg
     5521 aatgtggcag gtgagtggta gaagtgatat cacagacttc gaagaggttg taaaacttga
     5581 tgcagaatat atcaggaatt ggagtatggg attagatttt agaattttgt ttaggacagt
     5641 caaaactgtg cttcatagaa acggttccat gtaagataaa gatactatag aaggagtaaa
     5701 acgtgatggc aagaaaaaaa ttaaaaatct gtatgctagg tcataaaaga attccatcaa
     5761 gagaaggtgg aattgagata gtggtagaag aattggcaac gcgaatggtt gcacttgggc
     5821 atgaagtgac ttgctataat cgaagtggtc atcatgttag cggaaaggaa tttgaccagc
     5881 ataaaaataa agaatataaa ggtgtaaaat taaagactat ttttacatta aatataaaag
     5941 gaattgcagc aatgagttct tcagtatttg gtggaattag agcagcattc ggaaaatatg
     6001 atgttgttca ttttcatgca gaaggaccat gtgctatgtt gtggttgcca aaactttttg
     6061 ggaaaagatg tgttgcaaca attcatggtc tagatcatca aagagaaaaa tggaacaagc
     6121 ttgctagtac atacatcatg ttaggggaaa agtgtgctgt aaaatttgca gatgaaataa
     6181 ttgtacttag tgaaagcgtc caaaattatt ttgaagatat atatggaaga aaaacaaggt
     6241 ttataccaaa tggcgttaaa aaaattgaaa taaaatcagc aggattgatt acagaaaagt
     6301 atggattaac aaaagattct tatattttgt ttttggggcg attggttccc gaaaaaggaa
     6361 ttcggtatct aattgaggca tttaaagatg ttcagacaga taaaaaactt ataatagccg
     6421 gaggaagctc tgatacagat gaatttgcaa atgaactaaa ggaattagca aaaggtgatg
     6481 agcgaattat atttactgga tttgtacaag gacaggaatt agaagaatta tatagtaatg
     6541 cgtatatcta tacacttcca agtgatttag aaggaatgcc attaagtttg ttagaagcaa
     6601 tgagttatgg gaactgttgt atagtttcaa atatctcaga gtgtacggaa gtagtagaag
     6661 acaaggctat gatttttaag aaatcggatg taagtgattt aaagataaga ttagaagagg
     6721 catgcaatca atcggaaatg gttaaagttt tgaagaatca agctactgag tttatttgta
     6781 gtaaatataa ttgggataaa atcgtacaag aaacattaaa tttgtatagg ggaaatatat
     6841 aatgattcga atattacagt gtgtgaatga tatgcatcgt gcagggttgg agacgatgct
     6901 aatgaactat tataggaata tagatcgaac taaaattcag tttgattttt tgacacatcg
     6961 tccatataaa agtgattatg atgaggaaat aataagtctt ggaggaaagg tctattatgc
     7021 accgagactt tatccacaaa attatcctaa atattttaaa tggatggaac aattttttaa
     7081 ggatcatcca gaatatgaga ttgttcattc tcatattgat gcgatgagtt atttaccttt
     7141 aaaagctgca aaaaaagctg ggattccgat acggatagca catagccata atacatcgat
     7201 agatagagat tttaaatatc ttttaaagca atattatcga tgtaagttaa cgtctgtttt
     7261 gacacatgaa ttttcatgtg gaaaagtagc aggtgatttt ttgtttcgaa atgataaatt
     7321 cactttgatt ccaaatgcag tggatgctaa aaaattttat tatgatgaaa gtattagaaa
     7381 tgatgtacga atgaaattag gaatttcaag tcagacgttt gtaatgggac atattggaag
     7441 aatttcatat tctaaaaatc atcgtttttt gattgaaatt tttaaagaat ttcacgctat
     7501 taagaataat tccgtattat taattattgg aactggtgat atggaagaag aaataaaaaa
     7561 ttatgcaaag aaaagtggca tagatgatga tattaaattt ttaggaaata gaaatgatat
     7621 agaaaaattt tatcaagcat ttgacgtatt aatgttgcca tcattatttg aaggggttcc
     7681 gttagtgggt attgaagctc aatttgcaga tttaccgtgt ttcttttcgg agaaagttcc
     7741 tacagaagtt gctttcagtg ataagaccaa tttcataagt ttaaatcaat cggcaaaaga
     7801 atgggcgaga gagattgcaa atgtagatat ctctcatcga gattcagaac gttcgatttt
     7861 aataaaggca gattataata ttcaaacagc atacaaaatc ttagaatcaa aatattatga
     7921 gttatttgaa ttaatacaaa gaggttaaag aatatgacac agaaacattc aagtataaaa
     7981 agaattatac atcgcatatc cgaagaattg aatgcaacaa gacgaattgc aaagataaca
     8041 tcttggaagg aagcatttgc cacgttacaa gctaaaattg atattcaagt catgaatcgt
     8101 aatggatata atgaaagtaa aaaacagaag gaacatttat taaaaaaaca tgaggtaatg
     8161 ctgaagtatt atgaaaagac tttttcagat tttttgaaaa catatgatta tagtcaaaat
     8221 caaataatta atcaatcaaa atcaccatat tctaactgca tttgggtttg ttggtggcag
     8281 ggattggatc aatcgccgga aattgtaaaa gcttgtgtaa aatcaataaa acgaaatgca
     8341 gggaaacata ctgttataat tttaacagaa gataattata gacaatatgt tgatatacca
     8401 aaatgggtgg aagaaaagaa aaacaaagga atcattacga gaacaaatta ttctgattta
     8461 ttaagattat ctttgctagc aaaacatgga ggtttgtggt tagacgccac attttattgt
     8521 acaaaaccaa taccagatga gtattttgat atcgatattt ggtctattag gaggcctgat
     8581 tatgctcacg catccgtagc atcaggatat tttgcgggtt attcattagc atgtaatcaa
     8641 gagtctagat ggatgtttgc tataattaga gacttttttt taaattattg gaaaaataat
     8701 gatatgatgg tggattattt gatgatagat tatatgattg ttttagcaca gagaataaat
     8761 catgaaatag cacagacatt tgataaaata cctagtaaca atccattatg tgacgaattg
     8821 attaaaaatt taaataatat atatgatgaa aaagtatgga acttattaaa gaaagatact
     8881 ttactgttta agttgacatg gaaacaatct tttaaaaagc aaaaagatga taaagttact
     8941 ttttatggga tgttggtaga aaacaaatta ttataaatat tagaggtata aaaagttatg
     9001 aaacacaagg taagtgtgat agtaccaatt tacaatgttg aaaaatattt aagaaaatgt
     9061 gtggatagtt tattgagtca gacattaaaa gagatagaaa tcgtattagt tgatgatggt
     9121 tcgccagatg cttcggggga aatagcagat gagtatcaaa aaaaatattc taatgtaaaa
     9181 acaatacata gagaaaatgg ggggttagga ccggcaagaa atacaggaat tgagaatgcg
     9241 acaggagaat atgtggcttt tcttgattca gatgattggg tagaaagtga tatgtatgaa
     9301 aaattatact tagttgcgtc caaagaaaat gctgatatag tagttagtgg acattgtgat
     9361 tttgcgaatg acaaagctat ggtgaaaaag caacatcctt tgagtgggaa tgtatatgat
     9421 acgcctaata gtattaaaaa tgtacgaaaa aatttatttg gacatagtcc aaaagatcaa
     9481 gaagtagagg ccttcccgat gtcagtatgt atgtctttgt ataataaaaa tctgctagat
     9541 aaatatagac taagatttga aaaaatttta tctgaagata caatttttaa cttgaatgca
     9601 tatgatgtcg caaataaaat tgcttttaca gaatatacag attattgtta tagaaaagaa
     9661 gaacaagagt ctattacaaa aagtttatct ccaaataagc agacggaatt taaagaattt
     9721 ttaataactc taaaagctat agcatcgaaa gaagatttag aatgtcaaat cagagcaaaa
     9781 cgtatggcaa ttgattattg taggctttat gtgggcttag ttgatgatgc aaatttggga
     9841 attaaagaga aaagaaagta tataaaaaaa tttattgaag atgagggatt aattcaatgt
     9901 tggaagggat atcctacaaa tacgctacca tttcaacaaa aattttttca taaaatgctt
     9961 gaatgtaagt gttatacaat attgttgttg ttaagtagaa ttagaaagat aattaaaaat
//
LOCUS       DAYYDS010000090         9991 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000090 DAYYDS010000000
VERSION     DAYYDS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.093"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..321)
                     /locus_tag="ACHA02_002333"
     CDS             complement(<1..321)
                     /locus_tag="ACHA02_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8670634.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASM"
     gene            complement(450..1097)
                     /locus_tag="ACHA02_002334"
     CDS             complement(450..1097)
                     /locus_tag="ACHA02_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8670635.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(1181..2608)
                     /gene="uxaC"
                     /locus_tag="ACHA02_002335"
     CDS             complement(1181..2608)
                     /gene="uxaC"
                     /locus_tag="ACHA02_002335"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8670636.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(2682..3761)
                     /gene="uxuA"
                     /locus_tag="ACHA02_002336"
     CDS             complement(2682..3761)
                     /gene="uxuA"
                     /locus_tag="ACHA02_002336"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8670637.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(3764..5395)
                     /locus_tag="ACHA02_002337"
     CDS             complement(3764..5395)
                     /locus_tag="ACHA02_002337"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8670638.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            5712..6383
                     /locus_tag="ACHA02_002338"
     CDS             5712..6383
                     /locus_tag="ACHA02_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8670639.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(7219..8673)
                     /locus_tag="ACHA02_002339"
     CDS             complement(7219..8673)
                     /locus_tag="ACHA02_002339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670640.1"
                     /translation="MFDESLHLFPEYPDTVGSQAIPEKKQAVCQILLKEEVQLKQELT
                     AVILMDRICKEVLAGSDGTLAYGVVPLFDEVVRLLGNTQQIGAICGEFLLLPKMLVTV
                     ELAELATGMNEHPYMSGYFYLTVDDKNRQALMQAVTRLLTEKKLNILVGTVSLLGEGW
                     DCPAINLLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVPLSEQPDLK
                     RMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSCERSRLKEQ
                     WSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKGNLLEYFQI
                     RRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLENAGYREGQ
                     LFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQSSLPKCQLV
                     YTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(8674..8973)
                     /locus_tag="ACHA02_002340"
     CDS             complement(8674..8973)
                     /locus_tag="ACHA02_002340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670641.1"
                     /translation="MESQETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHN
                     YELIPEHWQLLGSSKQQGKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(9276..9647)
                     /locus_tag="ACHA02_002341"
     CDS             complement(9276..9647)
                     /locus_tag="ACHA02_002341"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8670642.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT         3123 a         2034 c         1457 g         3377 t
ORIGIN      
        1 catagaagct gatgccattg atgtttgata cattaaaaaa cttgctaaag cgactggacc
       61 actcatccgt aaaatccacg gtttaaattt gccagtttta gatggttttg atccatccac
      121 aatacgcccc attgttatgt ctgtaactgc atcaataaat cttgctacta aaaataatgt
      181 tcctacggta gctccgctta tccccaatac ttttgtataa aaaatcatta aaaaagaact
      241 agcaaaaatg aaagtgaaat cattcccaaa atctccaaac atatatccta ttttgtctct
      301 tagaccaaat tttctttcca tataatttgc gctccttcct attgttaagc gttttcattc
      361 tgttgaactg ccaacttacg tactatactc tgaagtataa agagttagta tttatttttt
      421 acattttata gtattattta ttttttctcc taataattct ttatctcata aaacttatct
      481 atatatttac tagctattcg agttacttct tcaaaatcac ctttttcaac aggagccaac
      541 aaattcccac ctacaccaac tgcaacaacg cctgcttcaa accactcttc taagttatct
      601 agacttactc cgccagtagg cattatattt acatatggta atggagcttg atacgttttt
      661 acaacagatg gaccaaaagc agaacctgga aatagtttaa tgatttctac tccagcggta
      721 agtgcctcct ttatctcagt aattgtcata caacctggta aataaggaat ttgatataaa
      781 ttacaaatct ctgctgtttg tcgatcaaaa gatggactta cgatatattc tgctccagcc
      841 ataattgcaa tacgagcagt aaacgcatct aaaactgtac cggctccaat tattacatct
      901 ttttgatcag catatttttt agcaatttct ttgattattt cttcagcatt cggaactgta
      961 aaagttagtt caatcccttt aattccaccc gcagtaattg catttattgc tttaatggcc
     1021 tcatctttat tttttccacg tacaaccgca atgactccag ccgcttctaa cttacttaaa
     1081 atttctactt ttttcatatc tatctccttc ttttatggat acatactcta gctagttgta
     1141 ctaacttaca ataaataaat ttaagctttt attttttgta ttattttcta ctttcgattt
     1201 caaaaaaatt aaaataatta tacgcattac gataacaaat attctccact atctgactaa
     1261 gatacatttc atcatctgga attcgacctg ctttaaccca ctcgcttagt aattgacata
     1321 atactcgacg gaaatactca tggcgaggat agcttagaaa acttcgagaa tcagtcaaca
     1381 tacctgtaaa attcgacaac aaactttgtt gcgccatcgt agtcaactgt tgacgcattc
     1441 cttcagctgt atcattgaac caccaagcac atcccaattg gattttttga atcatacccc
     1501 cttggaagct ttgcattgct gttgctagtg gaagccaatc ggttggattt aatgaatata
     1561 aaattgtttt tggtaagcac ccactctttt cagcagtgtc gaacaatgct acaacatttt
     1621 gtgtcaaact agcttgatcc cccatcgaat caccaccgac atctactcca atttgttcct
     1681 ttaatatact tgaattattt cttaaagcat ttaaatgcat ttgcatcgtc caaccatatc
     1741 ttgtatactc agacatcaaa tgtaattgaa tacctgtaac aaattgtgca atttctgata
     1801 aatggaaatc cgtttttcct gctaaagctt ttattaacag attgtcgagt attttttcat
     1861 ctgctatttc ataaaaataa gtattcaaac cgtgatctgc taatcgacag ccaatttgat
     1921 gaaaataatc cactcgttgg ctcaatgcct gcttcaaccc attaaagttt ttgattggta
     1981 tacccgctgc ttgactaagt tttaaaatat agttcgaaaa tcctgatgat ttgatactcc
     2041 agaccttatc tggacggaaa gtaggtaata ccttgaaaga attatcctgt ttagaaagct
     2101 cagtatgata tcgcaagtca tctgtaggat catccgttgt acaaatggct ttcacatcca
     2161 tcatcttcag caattctttt gggcgatagg aatccgtttg gattttttcg ttggcacgtc
     2221 gccaaatttc tggtgcattc ttctcagaaa ttgtcaaatc aatatcaaag taacgttgta
     2281 attccaaatg agaccattca aaaataggat tacctggcgc cttctccact gtttttacaa
     2341 aagctaaaaa tttttcataa tcattacctg caccactgat caactcttct tctatcccat
     2401 tagctcgcat caatcgccat ttataatgat ctccagctcc tttatcattt aaccatacct
     2461 gagtcaaatt cttgtattgc ttattttcta gcaaacatgc tgggtctaaa tgacaatgat
     2521 aatcgatgat tggttggtgt tctgcaaatt tatgaaaaag tcttttagct atttcattct
     2581 ctaataaaaa gttcttatct aaaaacatat ttaagcactc ctttccttcc cttagttttt
     2641 agaagagaaa atttttcttc taaatagact ccattatata attatttact cgcccccttt
     2701 tcaagcgctt cccaaatacc atttaaataa actgcaccta gagctcggtc atataaccca
     2761 taaccaggtc taccggtttc tccccaaatc atccgaccat gatctggacg aatatagcca
     2821 ttaaatccat tatcgtacat tgtttttaat atcttataca tatctagaga accatattct
     2881 gatttatgcg gcgcttcata aaaatcgagt ccattatcac ttaaatattt gatattacga
     2941 acatgtgcaa aaggaatccg atcccgttga caaaattcag atagaatttc atagacatca
     3001 ttatctggtc gttctgcaat acttccagta cataacgtaa taccattaga aggagaatcc
     3061 actacattac acaaccattc taaatcgtca cgatttttaa caatacgagg taatccgaaa
     3121 atagaatatg gtggatcatc tggatgaact gccattttaa tcccaacttc ttcacatgtc
     3181 gggataattg ctttgagaaa atatgcaaag ttctctcgca atttcgcttc atcaacttct
     3241 ttataggctt ctaataattg cttgatttgt gataaccgat gaggttccca acccggcaat
     3301 tcaaaattat tagtagaatt ttctactttt ctaataattt cctctggatc agtaggaata
     3361 ttttttgaaa tataggctaa cgtagaagac ccatcatcta aatgataatc caaatctgat
     3421 tttacccaat caaagactgg cataaaatta taacagataa cctcaatccc aaattctgcc
     3481 aaattgcgaa tcgtctgttt ataattctcg atgtattgat ctcgtgttgg caaaccaatt
     3541 ttgatatcat catgaacatt aacactctca ataactttca aggtaaggcc attggcttcg
     3601 acttcatctt ttaaagcttt aattcgttct ctcggccaga cctctccggc tggtatatcc
     3661 attaatgttc caacaattcc tttcattgct gggatctgac gaatttcttg tagtgagatt
     3721 ttttcttcat ttgccccata ccatctaaaa gtcatttcca tagttacttt cctccaactt
     3781 ctgttatata tttattgatt gttttacgaa ctgcttgagg acctactagc atttcgcaaa
     3841 agattttttc aattttcttt cctatctcaa cttgatataa atctaaacca aatatttcct
     3901 tattttctaa gatttcctgt accgcacaat gaatctttgc aggatcttgt tcacctaatt
     3961 tcaatactga gaatttttcc tgcaactcta ccaataaagg atcaggactt ggcacaaaat
     4021 tttttccttg atcatcgact gccaataaat aacgtaacca agcagcaata gcgataggaa
     4081 taaatgttag attttctgca tttccgtctt tttcgacata ctttttaatt gtttcaccat
     4141 agcgaatcgc tactttttga gaagtatctg ttgcaattcg ttgcggtgta tctggaatat
     4201 agggattagg caaacgttta ttgattactt catcaataaa ttgttttgga tcaataacgc
     4261 ctggactttc aactactggt aagccttcta cataacctat ccctttaatt aagccaacca
     4321 aatcatcatc ttgcatttct gcctgaattg atttataccc caacaaacaa cctgttattg
     4381 ctaatgccgt atggagcgga tttaaacatg tcgtaacctt catgatatct gctttatcaa
     4441 ctgtttctct atctgtcata atcacaccag ccgcttccag tttaggtcga ccatttggaa
     4501 aactatcttc aatgactaga taatggactt tttcagtatt ggtaaaagca gcgatattgg
     4561 tatttttcgc agtatgaatt atatctaagt cttcgaaacc cgccttcaat aaatccattt
     4621 tgacactttc cgaaggattt ggcgtaattc tatcaatcat actccaagga aaagtcactt
     4681 tttctttatc ctttaaataa gcaataaatt tttcagaaac gtatcctttt ttttgccaag
     4741 cttctgcaat cataaaaatg ctatcttgaa aacgttgacc attctgtgag aaattatcag
     4801 tagaaaccat cgcaatcggt aactctccag cattaaacct gctatacaac aaggatgtca
     4861 caatactcat cgtatgttga ggattggtag gtccagattt tatatctgct tccactaccg
     4921 aaaaaaattg tcctttagaa tcttttaaac tgtatccttt ttcagtaata gtaaacgttg
     4981 taaactgtaa tgtaggatct tcaaaatatt taaccactct ttgatagtcc gttatattct
     5041 ttggattgca atataaacta tcagctgtta ctgctaaaag tctttttttg agttcaccat
     5101 cttcacccat aacaatctcc aaaatatcat tgttgtagtc atgataagct ttttctacta
     5161 cttcttcatc aaatgtctca cacacaacta ctccggattc taattcattt tgatctgcta
     5221 agcattgagc aatttctgca tgaaatcctc gataaagatt tcctccacca aaatgaagcc
     5281 acactggatg tttctgacct tcttgtttga ccgcttcgac attaaaatac ggtagttgaa
     5341 cttgcatttt gttcaatcct tcgatagaat cttgccagtc tttaatcaaa accatattta
     5401 cccctccata ctgatatgtt agtttccgtt acaattgtag tataaaatcg aatgtaatcg
     5461 ctgtcaataa gaaaaaacta aaaatattct tttttctacc atgtcagaat tattgtgtct
     5521 ttttttagta aaagcacacc aatatatatt ttttagaact aacttaaatt aaagcttaat
     5581 ttctctttga tcttatttga gtattttttg tttttttaat agctagtatg ttactttgaa
     5641 atttgatatt atataacgag catagaacta aaatatcaaa ttcaaaaatg aaattttgag
     5701 agggattaaa tatgacaatt aaaactagtc tccaaaatca agcttatcaa tctattcgtc
     5761 agcaaattat ctacgctgac ttagaacctg gaaaaaaagt atcagaaaaa aaattggaag
     5821 aaacgctttc tatcggtcgt actccaatac gtgaagcatt gattcaactc cgtcatcaag
     5881 gacttgttga tacaattcct caaagtggaa attatatctc attaatcgac ttagatttag
     5941 caaaaaatgc tcgttttgtc agagaacatt tagaacgaca aattatgcta gagtgttgtg
     6001 ccaagatgaa taatcaaaag aaacaagttt tagaaactat aattgcagaa caagaaaaag
     6061 ctgttagtca aaagaatgct cgtagttttt ttcataatga taatttattt cataaagctt
     6121 gttttgaaat tgcagaacgg gaagaagtat ggcaatggtt ggaagataat aatacgcact
     6181 tggaacgatt tcgttggtta cgagtaaaaa cggaaggatt aaaatgggaa aaaattatgg
     6241 aacaacatca tcaactattt aaagccttga ccatgcaaga ccctgaagaa gctaacttct
     6301 taacatcctt gcatcttcat ttaatgcttg atgaacaaaa tgttgtattg actactcacc
     6361 caaaatattt taaaaatata taactgctaa atttcttttt tttaatttat ttacatttct
     6421 gattactaca gaaaactaac tacttttttt atgtaaagtt ctgtcaaata tagtcacttt
     6481 atattacttt ctcttgagta aaaaaacatg tgaggtcgta aacaaagatt gaactatcta
     6541 cattcatact ctataatctg tttatttgca gactgataat agcaaaagaa aacttataat
     6601 caaataatat caacatttga tcacataatt tgagattgaa tgctgattta cctatgtttt
     6661 acgtaatggt ctttagttat ttttgctcat aaaatgcata aaacacgcgt ttttatgtgt
     6721 gcttgaaatt tcttggctct ataatttcta agactgataa aaatattcta tcggttcttt
     6781 ttgcaaataa aaaaacatat agaagctcca ttagattgaa tcaccacaac acaatcaaag
     6841 gagcattccg tatgcttgat tttcattcta taaaactact tttaggaatc aaagaaaatc
     6901 acaacggttc aaactaaaag caagaatatc cgaagtacca ttgtttcatc gatcatgaga
     6961 aaagcattca cctctgtctg cagatgttct tatatcgctc tgtcagtcct attaataaag
     7021 tactgttttg ttttcaaaaa aactattacg agtacttttt aagagaatgc cttgcaaata
     7081 ttcatcacac accgttatat aagtcaaata gtttctttca gtcgtcaaga aaagattctc
     7141 ctgtccttgg cattatcagt taagtagcaa cttaattctc ggacttgttc tactctagca
     7201 aaatttattt tcatcaactt acgtccaaat gtttttttga ataattactt catcgcgggt
     7261 ctggaagact tgcctcattc gataagtcac tagcttttgg cgtccttcag gatttctagt
     7321 atacaccaac tgacatttgg gcagcgacga ttgatttaat tcgtttagaa aaaggttagc
     7381 gatttgctta tttcggccaa agcgttctgg taccgtcaga actatctttt tcccttcgat
     7441 taaataccgc ggattctcca gcggtgctaa aagctccctc atagcttcag taaaaagctg
     7501 tccttctcga tagccagcat tctcaagata gcatctaaat tcctgctgat cagccgctac
     7561 gattactttt ctatcactaa cttcaccaag gcgctgcaac gtcgtttgaa cgcatatggc
     7621 caagcttttt ttgcgggcac attccttttc ccatagcttt tgtcgcctac gaatttggaa
     7681 atattccagc aagttacctt taaatatagc tgaccagaag cttagaggct ctatattttt
     7741 tactactatc ggcaatgaag tttcttgttt ttgctttacc tgatcgactt tcctaacgac
     7801 gattggcata gataggtgcc agccatcagc taaagcagta ctccattgtt ctttcaaccg
     7861 acttcgttca caagaaaaat agaacatcga ctgagtataa gtttccatcg tggtggtcgt
     7921 atattccagt ggtaaagcaa agcgatcgcc accactctca atttttggtg aagattcaaa
     7981 ggtcagacct gaaatattgc ccaaacgccg tagcattctt ttcaaatcag gctgttcaga
     8041 cagaggaact cccggtaatc caatcgctaa atgccaaatg ttcgttactt tattttgtgc
     8101 acctaggcgt aagccccgcc ctcgtagttg ttgtgtttgc acaaaagtgg aagcttgatt
     8161 agccaagatc aacagattaa ttgcaggaca atcccagcct tctcctagca aactaacggt
     8221 tcctaccaaa atattcaact tcttttcggt taataggcga gtaactgctt gcatcaatgc
     8281 ttggcggttt ttgtcatcca ccgtcaaata aaaataaccc gacatataag gatgttcatt
     8341 catacccgtc gctagttctg ccagctcaac agttactagc attttcggta acagtaagaa
     8401 ctctccacag atggcaccga tttgctgagt gttacctaat agacgaacaa cttcatcaaa
     8461 caaaggaacc acgccataag ctaaggtacc atcactgcct gctaatacct ccttgcaaat
     8521 acgatccatc aaaatgaccg ctgtcagttc ttgcttcagc tgaacttctt cttttaacaa
     8581 aatttgacaa actgcttgct ttttctcagg aatcgcctga ctaccaacag tgtctggata
     8641 ctctggaaac aaatgcagcg actcatcaaa caatcatcca gcttttttca attgataaaa
     8701 aataggcaat tccggttgat cttcaaacat ctgctgataa atagtgatca gctctttttt
     8761 acctaaagat ggaactgaag cttttttacc ttgctgctta gaactgccta atagctgcca
     8821 atgttcgggt atcaattcat agttatgatg attcaagtaa attaatgcac tagtgaacag
     8881 ctcaaaattt tcataaattt ccttcgaatg ctcaagcgga tgctgaacaa attctaaaga
     8941 aagcaaataa tccaccgtct cttggctttc cattaacgct tccaccagct cattttgttt
     9001 ctctaaaaag gtaaaataat gctgagtagt ttcctgatta gccggaatca aatatagaaa
     9061 atcctgatga ggacatagaa cctcctcctt taccaactcg ggaatgctaa tttcttcgtc
     9121 aatcaggcca ttttaactca atatattgct tccactccgt ctgagagcta tcaatcggag
     9181 gcgtggctgt caaggaaatc gtcaacagat tcggttgttt tttcttcaag tcaatcaaca
     9241 attcacccca gctttttttt aaatgatgag cttcatcaaa aaactaacag ctccagctga
     9301 tcaccataag ttggctcctc cttcagtcgg ttgtaaatgg tctgatacgt ttccactgta
     9361 atatgactag gatgttcaat cgccccagaa agatagcaac tatctgctgg atttactaag
     9421 aaatattcct cgattgttgt taaccactgt ttcttcaata ttagggaagg aaccaaaatt
     9481 aaggttttac aatccaaacg tcggatcatc tcaattccta aaaaggtctt gccagcccct
     9541 ggtggagcca ctagatggac tgaattgtta tttaaataag tagcaaagtt agtcaatata
     9601 cgcttctgat agctacgcca ctgaattttt tcatcagata gccacatccc aggtccccct
     9661 ctcacgtatt ttctctatca taatctaaat gaatacagca aaaaaaagat ttcttcaaga
     9721 atatgtgttt ttatagtaat attttactta ctctaaactc aaatgaaaag gtaaaaaaac
     9781 tggtagtaca atcttttaaa ttctcattgt tttgtacgtt ctttactatc aaaaaaacaa
     9841 aaaaaacgcg tttctacgcg tgaccaaaat ttttagctct ataatttcta agactgatag
     9901 aaaatcattc tgtttttgaa gtcttttttc ataaatacta cttgatttat tgtgtatatt
     9961 gtataaaaga ttgttcataa ttatttttta t
//
LOCUS       DAYYDS010000091         9781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000091 DAYYDS010000000
VERSION     DAYYDS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.094"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(172..786)
                     /locus_tag="ACHA02_002342"
     CDS             complement(172..786)
                     /locus_tag="ACHA02_002342"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8670643.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(933..1616)
                     /locus_tag="ACHA02_002343"
     CDS             complement(933..1616)
                     /locus_tag="ACHA02_002343"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8670644.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1725..2183)
                     /locus_tag="ACHA02_002344"
     CDS             complement(1725..2183)
                     /locus_tag="ACHA02_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8670645.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2267..4030)
                     /locus_tag="ACHA02_002345"
     CDS             complement(2267..4030)
                     /locus_tag="ACHA02_002345"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8670646.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4251..5711)
                     /locus_tag="ACHA02_002346"
     CDS             complement(4251..5711)
                     /locus_tag="ACHA02_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670647.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5919..6845)
                     /gene="glsA"
                     /locus_tag="ACHA02_002347"
     CDS             complement(5919..6845)
                     /gene="glsA"
                     /locus_tag="ACHA02_002347"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8670648.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6868..8295)
                     /locus_tag="ACHA02_002348"
     CDS             complement(6868..8295)
                     /locus_tag="ACHA02_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8670649.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8326..9540)
                     /locus_tag="ACHA02_002349"
     CDS             complement(8326..9540)
                     /locus_tag="ACHA02_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8670650.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2902 a         2033 c         1621 g         3225 t
ORIGIN      
        1 tatttcctta ccatctaaag taacagtagt aacgacaaaa tggtcttgca atggcggaac
       61 ttctaagtac tgcccttgca gaaaatcatt cactgtttca tattcttttt tcatttcttt
      121 tgaatctgtc gatggtttat agcttatttt taacataact agtgctcctt tttattcggg
      181 acgtgcaatc gtgaaaattt ctcctattgc tgcataatca tttcctgcca aacggcttac
      241 agcattcaat tttcttggat cgatccttcc tgtcttatca tcatagactg tttcatttaa
      301 atgatatttc tgtacttcta gcaaaaataa atcagctgtt actttatttt tattccgtat
      361 ctcgacatgt tcatataagg atgtttcaaa acgaactttt gcttctgtca atgctggtac
      421 tgaaatgatt actgaatcag ttaaagtcaa gtcagtcagg tacagttcgc tttcctcatt
      481 aggcagattg gctgctgttt tatttacttc ttctacgttg tcctcatcaa ggatatggat
      541 aacagcttct cttgtctcaa gaatattctt tgctgtgtct tttactgcat cttgttttct
      601 ctggatcgcc aaggataaga tgggtcgatt cgaactgacg atattgaagt aactgaaggg
      661 tgcgatattc actatatctt ttgctgattt agtcgttact actgctactg gtctagggat
      721 gactgaacca atcagcaact tgtaattttc acgttctgtc aattctgaag gactccgttc
      781 aaacatggtt tcgccttctt tctataaaat tcgttaaaaa gcagatcttg aagacaaaat
      841 gaaaagtaat gcttttttca tcttcttaga atctgcttta aactatctat caatttttct
      901 tttttgtcaa gaatctgtgc cgtgcgtgtt ttttagttat ttgctttact tgcggcttcg
      961 acatatcttt tagtgatcaa ttgcggacgg gtgataatag atccaactac gactgaaaaa
     1021 gcacctaatt catatgcttt ctctacttgt tcaggagagt cagtatgtcc ttccataatt
     1081 actgggatat cgacttgttc tatgatttga ttcaataaat caaaatcagg tgtctgcgtc
     1141 tttcgtgaat attctgtata gcctgctaaa gtcgtagaca aaagatcaaa accatcttct
     1201 tgcgcttgaa gcgcttcctc aaaagtcgaa atatctgcca tagctaaccg accgtgttcg
     1261 tgaatcattt ggacaagttc agcagttgtt ttttctgtcg gacgtttacg agaagttgca
     1321 tccagtgcaa tgatttctgc ttgcgtctcc aaaagttctt ccacttcttt ttctgtcgca
     1381 gtaataaaca cttctgaccc atcgtaatct cttttgataa tccctattac cggtaaatca
     1441 acgattcgct tgatttcatt gatatcttct gtcgtatttg ctcgaatacc aactgctccg
     1501 ccttcttttg cagccaaagc cattctcccc ataataaaag aactgtgtag cggctcgttt
     1561 tccaaagctt gacaagaaac gacgactccg cctttgattt gttccagtgt atccacaata
     1621 tctcctccag taatgttata taaaaataca gcttcatata aaaatgtaac ccttttctat
     1681 tcaggagaca ataatgagct atttctttca gcagaccttt cttactaaaa gaaaattact
     1741 tcaaattctt cgtatacgac cagcttctct tctgtaaatt ctatgttcaa cgcttcttta
     1801 taagcatgtt taaaaaactc agcatggact ttccgccaat aagctaatgt ccgatcccct
     1861 tctccttctt tccaagcatg ctctttcgtg acttcactaa aaggcaagat ggtcacattt
     1921 atatttttga tgatacaaac tggttcattt tttccattca ggaccacgtc taattgacca
     1981 acttttggta aaggctcatt ctcttgttta tacagatcgt aaccacttga tgtaccagtc
     2041 tttatctttt ttacaactag ttcgctcaac tcgtctgcca ttttttctgt atccccaaat
     2101 gaaaagaaac ctaatttttc tgattgataa ctaggatatt tttcttggaa ctttttgaca
     2161 tattcttcta cttgctgata cataacatcc acccctttgc tttttataat gaaaaaacca
     2221 tccagatact agtataaaca attatctgga tggtgagcat aatgaattat tgaatattca
     2281 agacacgcag actatcttca acgatttgtt ttggtgttaa atgataacgt tcataaagct
     2341 gccctacagg aacgctatcc gtgaattctt tctctgcacc aaaatttaat acgttcattg
     2401 aagaatcacc ataataacga ctgatatttt cgccaaatcc tcctgacaaa ctaccatctt
     2461 ctaatgtgat gactaattcg tgtgtttctt ttaatttatc taatgtttct acgtcatttt
     2521 ctgtgatcgt tcctggattg atcaacgttg cttcgattcc tttttttcta aaagctttta
     2581 ctgtttcttt tcctaattca agaaaaccgc ctaaagctaa aattgctact tttcctccat
     2641 tatttactgt ttgataggct ggaatactat aatcatttgc gatagctgta ccatgttcta
     2701 caccatgtgt aggaattcgg attgctacag gctgcgctgt ttgttcgaac gcccaatcta
     2761 gcatagagat catatcctct tttgttgctg gagcaagata tttgatgttc gggatacttg
     2821 ataagtaagg aatgtcaaat gttccttggt gtgttcggtc actagctgag atcgttccgc
     2881 caccgactaa aataatcact ggattctcgt tgatcgctaa atcatgtgat aattgatcat
     2941 atgcccgttg caagaaagta cttgattgga agacgactgg acgtactccc tgagaagcca
     3001 aagcagaagc gaaagtgata gagtgctgtt cagcgatccc cacgtcaaaa taatgatctg
     3061 gatgtttttc ttggaatgtt ttcagtccaa aggcgcctgg tatcgctgca ttgactgcgg
     3121 caataggagc tgtacttgtt tgcattttct tatccaaata atccatgatg atcatattaa
     3181 atgtttctcc agaagttact tgctttgcct ccccggtgga cagatcaaat ggtacatgcc
     3241 aatgatattt cattttatta tcgattgccg gctgataacc gcgacctttt tccgtatgga
     3301 cgtgaagtac aatcggatga tcaatatctt ttacttcttc aaataaacgg atcaccgttt
     3361 ccagatcgtt tccattatcc acgtaacgat aatcaaatcc cattgcttta aataggttat
     3421 tgctgctttt accgttggtt tgacgaagct cagctaagtt tttatataaa ctgccatgat
     3481 tctcatcaat agacatctca ttatcgttta ctaatacgat tagattggat tgtaaaacac
     3541 caccattgtt caatccttca aaagctaagc caccacttag ggagccgtca ccgataaatg
     3601 ctaccacatt tcctgattct ttcttcagat ctctcgcttt agctaaacct gttgctaagc
     3661 tgatagatgt agatgtgtgc cccactgtaa aatagtcatg tttactttca tgttggttgg
     3721 aatatccgct cacttcacgg taatgtcctt tttcaaaacc attttttcga ccagttaaaa
     3781 gcttatgacc ataagactga tgggaaacat cccaaacgat tttatccttt ggtgaatcaa
     3841 atacataatg aaacgcaatc gttaattcta ctacacctag attcggacca acgtgcccac
     3901 ctacatcgct gtccttttgc aagatttttt ggcgcatctc ttctgccaac tgtttcattt
     3961 cctcaacaga taattttttg acatctgcgg ggctattgat tgcatccaat acatgtaata
     4021 ctgtactcat aatacattct ctcctccgta ctatcttctt ttaatgaaaa tagatttgtt
     4081 acactttatc tattctacta cttagagttg actctaagtc aatagcattt taaaaaaatt
     4141 tcagagaaag cttgttatat aagtagatct tcgcacgaat caggactcaa ataaaaacct
     4201 tctacagaca attaaaaagc tttgtagaag gtctcctaat taaggtctca tcaagaaata
     4261 tgttttttta ataggtgaag ttcctgagca atatgataca aatggatgtt tgcacctttt
     4321 aatgagattg gatagatctt tgtcttttca gtttctaatc cattttgata gctagcagca
     4381 tcgcacaaaa ccactctttt tttcgcacat agacattcat caggcaattc ttctaatgaa
     4441 cgaaataaaa taggatttat ttcagcgatg ccttcttccg catacaaatg aaattgggtt
     4501 cgataaaaaa attgtttgac tgaactttct gaaacaacaa aaatttctat ttcctgtctc
     4561 cccgcttgtt cactgttgat caaagaaaga cttttgacga attttcgaat gaaattttca
     4621 ttccatctta gttggatttg atttttcttt ttccactctt ccaatatttc tacaattttt
     4681 ccgtacagaa gttgttcttt ttctgataga agatagatcc tttccggtaa aaatacttgt
     4741 ccatctgccc acaacgtacg gacaaattgt aaaaaggatt gcttgaaaat aaaattttct
     4801 gcatcgtcga tttcagcgat actaataatc tgtaaaatca attcattcag tgataggtca
     4861 ttgagaacaa aatttttata aacaacttcg aaatcttttc ttaacagctc catgttctca
     4921 ttggtataat ttctactgtt gaacaaagaa aaaatataat agacatcttc ttcttgtaag
     4981 tctagaattc ctttttgctc ttccaatcct tgacagacta attgaaataa cggcttcgtt
     5041 tctgcgcttt tcttatcttc ttctttcagt ccaaggggaa atgcctttct tccgattgcg
     5101 atctctattc ctcgtatgat aaaggacttt tcatcttccg aatatcttct tttaaaaaaa
     5161 tgagtctcta cataatcaat caactcgtta caagcttctt ctacatacgg atttttattt
     5221 tcatagcctt cccaattgac ataacgtact aaagctagca agatgttccg cgagtcaaat
     5281 tctgggataa tgatttcttt atttttcaaa taatcatttt ctttaaaaaa atccaatacg
     5341 atttttttga ttgtatatac tttgctcaaa gaaataaatt caagatcagc cagttctgta
     5401 ctcgtgaata taccattaaa ataataactt aaaaacttta aaaaataaga ttgaccgtac
     5461 agaaactgtg tgagctcaga aagagcaata ccatgtttga atacgagttt atacttatgc
     5521 ttctctctta cttgttcttt tatacaatat tctagacact cggctagact ttctagatcg
     5581 tgaaggatcg ttggtgtagt cacccttaga gaaatagctg tttgttcgat gtctatttct
     5641 tggaattcaa ataattgttc acacagatac acttttctaa atatatttcg ctcgatgtaa
     5701 ttttccagca ttcctgatac acgcctccag tttttcttat gattttattt aatcatatct
     5761 gtaaatcgta tttttgtttc ctttttattt agaaaagccc tttcaaaaac caataaaaaa
     5821 agaaactggg acaaaagtca aacaagctca caatttcagc ctcattgtag gtagctttga
     5881 gccgtttgga cttttacccc agcctctttc aagaatggtt attcaaagat gtcagcgtct
     5941 agttctttga ctacatcttt caacaattgg ataccagctg cgctgttacc aaatggatct
     6001 agtgccgggc tgaagacacc tatgccgtaa cgatttggca cagctgccat caacccgccg
     6061 cctacaccgc tttttgcagg tacaccaaca tggacggaaa actctcctga ttcatcatac
     6121 aaaccagcag ttgtcataat tgatttgacg attgtagcac tttcttcagt aatgatttgt
     6181 ttgccattcc aaggagcaat tcctttattt gccagcacag cagctaattt tgctatgcct
     6241 gtagcggtga cattgaggga gcattgtctg aaataagcat cgagtatctc cggtacgtct
     6301 ccttcgatca tttgattacc tttcatatag taagcaagag agcgattgat atcacctgtt
     6361 cttgattcag aatgatagat ttcttcattc aatgtgatat taggatcatc acagatctct
     6421 ttcataaatg acaagattcg ctcaaatttt tcatctgcat ctttaccggc aatcaacgag
     6481 gttgttgcaa tcgctcctgc attaacaaaa ggattcattg gatgtttgcg atgattgatt
     6541 tccatattca tgatagaatt gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag
     6601 actttttcta ttccgttgtc tttgattgct aataacaata cgggaacttt tgacatgctt
     6661 tcgatggcaa agcgcacatc ggcgtctcct ccctcaatca gttgttttgt atccaaatca
     6721 taaatcgcca tacctaattg tttgggatcc acttcagcta atgctggaat gtaatccgcc
     6781 acttgtcctg tttgataata gtggaaattt tttccaatca attgttctaa ttcttttact
     6841 tccatttgaa taaccagctt tcatttttta tttttctgca tgctggaatt tatgacggca
     6901 tgcgtagatc gtaaatggaa taactaagac gataagataa gaaacaatca gaatcgtttc
     6961 ataaatccca tgctgtccag ctgcgatact agcaggtgga acaaatgaga tacaaaaagc
     7021 aaaaagtgaa gtcaaaagac ctaaacaagc aacgattact tttacaactt ttccgcctgg
     7081 aacctgatag gctctttcaa gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa
     7141 aaacataaag tacaacatca ggtagattac aacagtcaat gacatcgctg caagaaatga
     7201 aagattattc cccccgccgc ttaatgtaaa gacggctgcc cagattgttg caacgatccc
     7261 atttgcgatc atcaaatgag caggaacacc gtgtgtgttt tcttttgcaa aatattcagg
     7321 aagtaatcct tcttctgctg ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc
     7381 aatttctccg atgacaccta aacaaacaag aatcgcaaga attttcgcaa tccatgcaag
     7441 atttggatca atgtgtctta ataggaattc taacgattga atcacccctg tattaagaga
     7501 caaaccatgt tgaggtactg tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat
     7561 tgccagaata accaatacaa acattgctaa tggataatcc tttttagggt tttccatttc
     7621 atttgcatga ctggcagatg tttctacgcc catatatgct aaaataaagg aaacaaaaac
     7681 aaccagcgta ggcaatttcg aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg
     7741 tagtggagcg cctgaagcga cataatggat ccctaagcca aacaagacta atgctggcaa
     7801 caagatacca gctacaaaac cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa
     7861 ctgtgagagt aaaatagccc agaaaataat caataggatt gccaatttca gatatttatt
     7921 gttttgtata gcaccaatac ccgttgcata ggataatgca ccagttaaga aatacagcat
     7981 ggtcaagtag ccaactgtta tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg
     8041 ttttcccaat gtgcgactga cccaagtata gacaccgcct ttttcccatc ctttcaccgt
     8101 tgccatctct gctgcgcaca atgccacagg gataaaccat agcaaaccgc ctaataacaa
     8161 gaaaaatact aagctgaagc ctgaggtcgc gaatgtggga tattcatata atgaaacgac
     8221 cattgagatg gtcatagcca aaaagccaaa gaacgttaat ttttttgcag gaattttcgt
     8281 tttgctttgt tgcatgaatc ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt
     8341 gccaaagaag aaaaagtcca atccttcttc ttgttcttct tttgtagtag tgatcgttgt
     8401 aaagtgcatc gccgatttta aaatcactgt tgtcagcaca taggcataca cggcagtaat
     8461 gatgacacca atcacttgaa tccctaagaa gtaccagctg cttggattcg ttagtaaatg
     8521 gctgttagat gcaaagatcc ctatcagaat cgtacctgtg aatccgccca tgccatgaac
     8581 accccacaca tcaagtgcat catcccattg tttcttttta cgaaaatcaa cagcagcatg
     8641 acagacgata cccgcaatga tcccaatcag catggcactt tttgcttcta cataaccggc
     8701 acaaggcgta atggctgcca atccagcaac ggatcctgtt agtacgtcga caaaatcaaa
     8761 gccatttcct tttacttttg caatgatcag ccaagtaatc atcgcaaatg caagagcgat
     8821 gaatgttgaa ccaaatgcat tgactgcttg ttcatctgct cgaagtgcac caccggaatt
     8881 aaaaccgaac cagccaaacc aaagcagtcc tgtaccgata gcagctgcca taagactgct
     8941 atgctgactt ctttctccta aaggaatctt tctttgacca agtgtgagga tacatgctaa
     9001 agagccgaat ccagctgtcg tgtggatgac tgctccacca gcgaaatctc taaatcccat
     9061 ctgatccaaa aatccgccac cccatatcca gtggcaaaca gggaaataaa tcagcagatt
     9121 ccaaaaaata acaagtataa tatagccttt taaattcaat ctccctgcaa aagctcctgt
     9181 catcaaagga acggtaatca cgataaacat cagttggtaa agaaagaaca ttaaaaatgg
     9241 aatcgttgca ccatgaaaca tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc
     9301 tgccgggttt ccaataacac cgccgatatc ccgtccaaag gccaatccga atccaccaaa
     9361 tacccacata atcgttgtta tcccaacgga gagaaaagat tgagacatga tcgttagtat
     9421 attattcttc cgtgctaatc cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac
     9481 cattgctgta cataaaatca tgaatgctgt atctcctgta ttgataggta atgtattcat
     9541 ttttttcgcc tcctttctac gattacgagt ataaaagaag gtgtagttgt ttttttcttc
     9601 tgtttttttg aattaatact tgcatttata aaagacagac aattaataaa agcttgatgt
     9661 gttgtgctct ttttcgtttt tcacacaaat aaagacaaaa aataaacggt cttcttttta
     9721 tagaaaaccg tttattttcg tcttttttta tttttaaaga tattttttca taaggaataa
     9781 a
//
LOCUS       DAYYDS010000092         9741 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000092 DAYYDS010000000
VERSION     DAYYDS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.095"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACHA02_002350"
     CDS             33..656
                     /locus_tag="ACHA02_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670651.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACHA02_002351"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACHA02_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8670652.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACHA02_002352"
     CDS             2152..3033
                     /locus_tag="ACHA02_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8670653.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACHA02_002353"
     CDS             3036..3806
                     /locus_tag="ACHA02_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8670654.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACHA02_002354"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACHA02_002354"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8670655.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACHA02_002355"
     CDS             5117..6280
                     /locus_tag="ACHA02_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8670656.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACHA02_002356"
     CDS             6397..6807
                     /locus_tag="ACHA02_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8670657.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACHA02_002357"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACHA02_002357"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8670658.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACHA02_002358"
     CDS             9380..9664
                     /locus_tag="ACHA02_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670659.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3385 a         1649 c         2090 g         2617 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata a
//
LOCUS       DAYYDS010000093         9357 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000093 DAYYDS010000000
VERSION     DAYYDS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.096"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHA02_002359"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHA02_002359"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8670660.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHA02_002360"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHA02_002360"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8670661.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHA02_002361"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHA02_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8670662.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHA02_002362"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHA02_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8670663.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHA02_002363"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHA02_002363"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8670664.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHA02_002364"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHA02_002364"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8670665.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACHA02_002365"
     CDS             complement(8053..8523)
                     /locus_tag="ACHA02_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8670666.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACHA02_002366"
     CDS             complement(8569..8994)
                     /locus_tag="ACHA02_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8670667.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACHA02_002367"
     CDS             complement(9061..9330)
                     /locus_tag="ACHA02_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8670668.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
BASE COUNT         2355 a         2095 c         1621 g         3286 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttt
//
LOCUS       DAYYDS010000094         9352 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000094 DAYYDS010000000
VERSION     DAYYDS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.097"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..751)
                     /locus_tag="ACHA02_002368"
     CDS             complement(83..751)
                     /locus_tag="ACHA02_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8670669.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            885..1172
                     /locus_tag="ACHA02_002369"
     CDS             885..1172
                     /locus_tag="ACHA02_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670670.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1588..3126
                     /locus_tag="ACHA02_002370"
     CDS             1588..3126
                     /locus_tag="ACHA02_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8670671.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3123..4088
                     /locus_tag="ACHA02_002371"
     CDS             3123..4088
                     /locus_tag="ACHA02_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8670672.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4075..5397
                     /locus_tag="ACHA02_002372"
     CDS             4075..5397
                     /locus_tag="ACHA02_002372"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8670673.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5460..5564
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5638..6381
                     /locus_tag="ACHA02_002373"
     CDS             5638..6381
                     /locus_tag="ACHA02_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8670674.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6396..7388
                     /locus_tag="ACHA02_002374"
     CDS             6396..7388
                     /locus_tag="ACHA02_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8670675.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7400..8137
                     /locus_tag="ACHA02_002375"
     CDS             7400..8137
                     /locus_tag="ACHA02_002375"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8670676.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8127..8849
                     /locus_tag="ACHA02_002376"
     CDS             8127..8849
                     /locus_tag="ACHA02_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8670677.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8933..>9352)
                     /locus_tag="ACHA02_002377"
     CDS             complement(8933..>9352)
                     /locus_tag="ACHA02_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8670678.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3067 a         1607 c         1972 g         2706 t
ORIGIN      
        1 aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc tgtaaattaa
       61 ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca agtggttcaa
      121 atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt tccagatact
      181 tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac agaaaatgtg
      241 tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag aaaagacttt
      301 tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct gttccgcctg
      361 tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata cctgtcatca
      421 aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc cataaatgta
      481 aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt tttccaaaaa
      541 gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca gaaggagcga
      601 atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct gccagcttag
      661 atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa atgattccgc
      721 acaattctgt aaataaattt aattttttca tttctagcct cttttctttt gaaaattttc
      781 agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg aaaaaaaatt
      841 atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga gcagcagact
      901 tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt tttttggctc
      961 aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat caatttatct
     1021 ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt tttttgatga
     1081 agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct ctttatggag
     1141 tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa gacgtttatg
     1201 atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac agtataatat
     1261 tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta ttggctgttt
     1321 ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc aagtgacaat
     1381 ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag ttgatccgac
     1441 tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc ttttttttgt
     1501 tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa atgtttatac
     1561 ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt aagtgaaaga
     1621 aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat ttctttgctg
     1681 attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa aaaaatggca
     1741 gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc cttattaggt
     1801 ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact tgttatttac
     1861 gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga cccgtctatt
     1921 gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa agtagagctt
     1981 cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt aatcatcggt
     2041 acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat tttgttaggt
     2101 attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc cttgcttgca
     2161 attatattca gcggactgat tcgctggttg cagcataaga aacctcgata ttccatcatt
     2221 actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc tgccttgttt
     2281 aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat cctgatccaa
     2341 atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa accgaatttt
     2401 ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat ctatcctgag
     2461 ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa aagtgctgat
     2521 ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca attcgatatg
     2581 acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa gagatctttt
     2641 gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga tcagatcaaa
     2701 gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat ccaagaagca
     2761 tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta ccaagcaatc
     2821 aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct caaggagtac
     2881 gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg cgcaccgctg
     2941 atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa taaattaagc
     3001 ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt cgaaaagaaa
     3061 aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa ggaggaagat
     3121 cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga aagtatgtca
     3181 tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt ggtccttccg
     3241 gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca tcagcaggag
     3301 acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg cggtggaata
     3361 tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag caaaatattg
     3421 aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt gtagatgaac
     3481 ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct agagagttgt
     3541 ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc ccagatgtga
     3601 ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca ttacaagaat
     3661 tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg cacaatatgg
     3721 aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa attatccaat
     3781 gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg catttctttg
     3841 atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat ggatatttct
     3901 taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct atgaaagaag
     3961 tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa agaagtattg
     4021 ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa ggaaatgaaa
     4081 acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc ttatggattg
     4141 aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg gacttgtcct
     4201 aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg taatcgggta
     4261 aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga agaacttcaa
     4321 aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca attagcagaa
     4381 gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc tattgaagtc
     4441 aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag acctacgatc
     4501 ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc attgcctgta
     4561 ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct agctacgatt
     4621 gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc attaaaagaa
     4681 tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg cattcagttt
     4741 gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg tttagtcgga
     4801 aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca gccgaataca
     4861 gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat tgcagtcaat
     4921 gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt ttcaaggaaa
     4981 accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa gcggataacg
     5041 gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta cgccagtcct
     5101 aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca agtggtagag
     5161 atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc taaggctgaa
     5221 ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga gcaagcagac
     5281 gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa agagttggat
     5341 agctatatca tggggtatcc gacattggca agtgatctct cctatttatt gaaataatca
     5401 gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta aaaaatgaat
     5461 acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta gacccattga
     5521 acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc gggttactat
     5581 gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg taacaaaatg
     5641 aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg gcagattgtt
     5701 gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt attttctgca
     5761 ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa tgaagcagtg
     5821 cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat ggattattgg
     5881 aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt gccaatcatg
     5941 gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa agtgatcctt
     6001 gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt gaggaaagta
     6061 tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa gatttatcga
     6121 atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt ggctgctact
     6181 tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg cttagggtat
     6241 tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc gatcatttgt
     6301 gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa agggtattat
     6361 cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg cttttattac
     6421 cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc aaacaagaaa
     6481 taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact aatcatactg
     6541 gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat gtccaaatca
     6601 tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa gcacaatttg
     6661 gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat ataccagtca
     6721 aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta tcagacagta
     6781 acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa agtccaagtg
     6841 aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca aaactgacaa
     6901 tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat atccaatggt
     6961 actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta aactatatcc
     7021 cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt acgaatgatc
     7081 aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg aaaaaaggct
     7141 atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa tatgcaaaag
     7201 aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat tatacggatg
     7261 atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca gcttttatgc
     7321 aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact aatcagttta
     7381 taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag tcattgaaaa
     7441 cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta catcgattgt
     7501 cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag tccagcctat
     7561 gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata cagcatttag
     7621 ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa acgttacgtt
     7681 atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt atctggatat
     7741 ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg ggatgaagca
     7801 gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc ttgatgaacc
     7861 atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt tgaagctgcc
     7921 taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag caatttattt
     7981 atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg agattcaagt
     8041 gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga agcaaacgat
     8101 ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat ttagatggga
     8161 gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc aattgtgatt
     8221 cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa gccttaagtt
     8281 acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag ccttttttga
     8341 aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat gtaccgatga
     8401 ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat gagaaacctg
     8461 ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa gcttacccta
     8521 atcactattt gatccattgg tattcttctt tggactatca acaagactat attgatttag
     8581 gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg caactagcta
     8641 aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca gcagagtggg
     8701 ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa cagttaaaga
     8761 caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact tatcaaaact
     8821 tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta gtgtcgcacc
     8881 tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa tcttagtacc
     8941 aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt gaagtaacat
     9001 attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag taagaagaat
     9061 ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg cttgattctt
     9121 tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca gtttctgtta
     9181 cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt tgaaccgttt
     9241 cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct tgaggttcag
     9301 cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag ta
//
LOCUS       DAYYDS010000095         9345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000095 DAYYDS010000000
VERSION     DAYYDS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.098"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..381)
                     /locus_tag="ACHA02_002378"
     CDS             complement(<1..381)
                     /locus_tag="ACHA02_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8670679.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTF"
     gene            complement(371..2545)
                     /locus_tag="ACHA02_002379"
     CDS             complement(371..2545)
                     /locus_tag="ACHA02_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFM8670680.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2561..4903)
                     /locus_tag="ACHA02_002380"
     CDS             complement(2561..4903)
                     /locus_tag="ACHA02_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8670681.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4925..7468)
                     /locus_tag="ACHA02_002381"
     CDS             complement(4925..7468)
                     /locus_tag="ACHA02_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8670682.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7478..7876)
                     /locus_tag="ACHA02_002382"
     CDS             complement(7478..7876)
                     /locus_tag="ACHA02_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8670683.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7957..8241)
                     /locus_tag="ACHA02_002383"
     CDS             complement(7957..8241)
                     /locus_tag="ACHA02_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670684.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8265..9263)
                     /locus_tag="ACHA02_002384"
     CDS             complement(8265..9263)
                     /locus_tag="ACHA02_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8670685.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2655 a         2002 c         1463 g         3225 t
ORIGIN      
        1 gaatgttttt ctctcaattt ttttgacgtt tctatcatga atcacgagaa aacttgggac
       61 acttttaaca tggtaacgtt gtaaaaaagt ttgaagttct tcttgtcttg gttcttttct
      121 tgtttcaatt cgatatacat tcctagagaa ttttttgtcg tatctttcca agaaagattc
      181 aacttcttgg cattttttac aatcatttct tgctagatac acaaaaaatg attgattcgt
      241 tttttgaagt tctagtactt cttctaaatt actattgatt agttttccat ttgattcttc
      301 gttagttttt atcagtttaa aacaaccaaa taagaggaaa ctaacaataa gtatcctaac
      361 aataactttc ttatttcgca tattgtttct ccaaatttgg tttccgaata tagacgactc
      421 gcggtggttc ctggacgtct tcagctgttc gatacaccac aaattcgcct gttctcaatg
      481 ctcgaatgcg attcggattg aatttaaagc ggtctacgtc tcgtttcgtg cctctttctc
      541 ctgtccaatc aacctttccc atatcagaat aacctgcttc ttgtgtcgtc atttccgtta
      601 attcaatgtc ttcttctgtc cccattaaat ctgcccaata atcaatttct gtcgggtcat
      661 ttgtcttccc aataaagaac gtattcacat tcccaatcaa ctgttttcga aggacaggat
      721 caattctatc aatgtccgca agcgtttgag gggcatacgc cgcatgcacc ccagcgcctc
      781 gtcccttatt gactaagtct ataaggttat catttagata agaagctggt tcgtcaaata
      841 ttactaggac tttcgagcga ttggctgttt ctcctgcttg tttatacact tctgacgcaa
      901 agaaattaat atctcccaca agcatttgag ccaatgtccg aataaactca ctataaatca
      961 atccgtttaa cgagatataa acaatctttc cttctttaac aacttcttga atatctaatt
     1021 ctctttttcc tccagaagta tcaaagagtt caccaagctc actatccaat aacatattga
     1081 tattggtctg taaattctca gaagtcgcat acaaataaaa cgggttcttc ttatgttcgt
     1141 aacgaacaaa caaccgttta aacaacgttt gctcgatttc tgtcatgaga gaaatgtttt
     1201 cttttaataa tagataatac gtttctaagt caacggtttt acgatcatgt tcaacgattc
     1261 gctcttcact taactgttcc acatcggcaa aatcacgttc tgttttgtcc atcgcttcat
     1321 tctttttcgt ttttttcttc gtggttttct tcttttctac gaccactttt tctttaaata
     1381 aatctggatt tttcaactga atacgatccg caaacaaatg taagacgttt cgtggcaata
     1441 aataatgttg taaccaaact agatttcttt caattttatg gctcttttgg aactcgtcta
     1501 aaagctgaat cgttacctgt aataacgctt tggctgctgt cgaataatac acgctttctg
     1561 tttctgctag attggttaat ttatctcgaa tggcaatatc attgccattt tttacaggat
     1621 tataacgcaa atcgccaccg tccacaaaga cttcaatcgg gcgattgtat gcctctgcca
     1681 cttcttttaa cgcttgtaaa gtctctcctg ctcctttccc atcgactaag ataacgggga
     1741 tacgaaactt acacgcatgt tctgctagta attggatcac cgtcgttttt cctgaacctg
     1801 tagtcgctgg aataatcaca tgttggttca attcactaaa gttcatcaca taagggagcc
     1861 catactcatc tgttcctaat agaatcactt tggctaatga agccagttgt ttattccgca
     1921 aagcaacgtc ttttatgaca attgctacct ggtacgcttg ctgatactct gctgtatatt
     1981 tttgggactc ctgaaattta ttcttccgac gttccttgta ttctttacta tttagcaaag
     2041 ctgtttgccg ttctttcttt gaaacaactt ttcttttttc aataaaagac gcatacgcaa
     2101 tcaaacaact tcctgcacct gtcccaccta aaaggagggc aaaaatcgtt tgtccattaa
     2161 actgcaacgc atgagaaaac aagtttaatg gaaataataa gtaacacgac aaataccctc
     2221 taggaagaac aaaaataaaa caaaatagat ttaatagcgt aaaacctaac gcaaaaataa
     2281 aaaaaagaga gcttcttcgt tttaagaagc tctcatactc aaagtcataa atatgtgcac
     2341 gtgtctcttt tttatctatc ataaagacaa tcgctaaagg aatactgcct acaataaaaa
     2401 atggatatag acaaacacct aataaagtga cgataaaata aagttgttga aaccgacttt
     2461 ttggcgtttc tttttcttgt tttcttttgc gatcgacaga aaacatttgc tcaaaatgtg
     2521 tcggtcgttt tggtgcattc atcatactat cgatttcctc ctatcgtttt cgttgtttca
     2581 tttctcttcg ataaatcggt tgtttttctc gttttcgttt catttttgct tgagttgctc
     2641 gaaactcact ccgtgtcttc ggtgcttgtt ttggtgattt tgtttgtttc atggttgagt
     2701 atgctttaga tacggttgaa ggcgctttgg tttgtgctgt tactcgattg actgctgaca
     2761 tatgttcccg aaccccctct ggacgtcgaa cagatttcgg tgtggtagaa cctgttgctc
     2821 gtggtacatt cccttttctt agtggttttt gtaccgcatg tttttgtcct gcaactgtcc
     2881 gggttttcat cgaacttgtt tttgttacgg gagtaagtgc ctccttccga cgacgacttg
     2941 tttgtccctg acgacgtttt cgatcgttca attctgttct aggcgtatgc ttctggccat
     3001 aagacttgtg tttttgcttt ggattcgcta atgacaccac cattgcagaa ccctgattgc
     3061 gatacggact acgagacgca ccttttctac ttctttctct tagactagga ttgccttgca
     3121 attctcgttg taataaatcg gctgtttttc ttgcctgttt ctccattgct gttcgttgca
     3181 tacgttcttg actttgctct tctgcttgtt tcgcttgatc atgcaagtct cttgcttttt
     3241 gtgccatacg ttccatacga tttggtgctt tatgacgagg ttgttgtgcg gattcacgcc
     3301 gaaaagcact tcgtgtggct tgtttcgccg tttgtggcat acgaacgtct gagccacccc
     3361 cacgttttcg ttgacgttct tgtctcttcc gtgcgttttc tttttctttt tgtttctgtg
     3421 cttttctcgc ttctttgcgt tctttcaacc gttgtttggc ttcccttctc aattttgctt
     3481 ctgccccaaa tggatttccc gcaaaagcta acgctcctcg aacacccatt cttccagtaa
     3541 atagactgcc taaaaagcct cggaacaaat aaattcccaa cggcgcaaac aaataaacaa
     3601 tgatcaagag aattttttcc catggatcgt cttgtgtact acttaattgg aacccgtaac
     3661 taagaaaaga agaaaaaagg atacatgcaa aacctgtcat tccttttaaa aacgtcaaaa
     3721 taccaaaaac tttggcatag ttaccaaaca cattcattgt gacaagaaac aatcctaaaa
     3781 aaagtaagaa cggcaacatc ggtactaaca atagttgcat agttccaata atcaatcgaa
     3841 acatacttaa gacaaaataa acaacggttt gaatcagatt cacgacaaaa taaaacaagg
     3901 caaatgctgt atttttccaa ctattgtaat attgtatgtt cttgtttttc agtgtatctg
     3961 cttcatactc tgtgactttg tcatgaattt tctcccctat ttttgtacct aaatcattgt
     4021 ccaataaaag attgattcga tcatattctt ttccagcata tttgactttc ttctcacgaa
     4081 ttgtgtctac attggttgtg ccataattta acaacagata tggttcataa acattactat
     4141 aaaagacccg agccgccatc aatttgcctg ccgtttccac ttttttatcc gctgttacct
     4201 cttctgtttc ttttgtttca taatttgttt cttcttcagg tgttaacact ggattgacct
     4261 tcacgaacgc cgtttccact gtagaatcta aagaaaaaag aacatcaaaa agattcttat
     4321 ttgtcccaga atcttttaat acgcttaacc cagtaaaaat aaggatcgtc ataagaaaga
     4381 cacggagtgc ttgtttcatt cgagcctctc cagcatattt gattgccatc ataattgccg
     4441 aaaaactgat tccaatagtt cctgcaatac ttaacatatt gtccgcaatt ttttgtgtta
     4501 agttttgaat gggttctcga atggcggaaa taatatctaa ttcaaagaga aattcaacca
     4561 ttgaagcatt gaattctcct aacattttta cgccactcca aacaaaattt ttcatcactc
     4621 ctgcaacatt gtacgatagc ttttttttaa ggttcattga ctcatagtca ttggtcatca
     4681 attcaaacga attctttttg taatttttat acaattgatc cacatcagtc gttacttctt
     4741 ttacttctac tttatcgttg gcattctctc cttcagaacg atcggtttca gtgacacccg
     4801 caccatcatt catttgatcg gcagaaacag tagccccttg tagacagaaa aaaagacccc
     4861 ctaataaaag gagcgtccac catttcagag atttttttcg caaaaaaacc acctcttttt
     4921 tgttttatcg ttgtttttca ttttctaaca ttgcttgaat tttttcatct tgattttcag
     4981 tagaagaaac agccaataac caactatcaa acaacacatc aatggcaatc ggttgatttc
     5041 ttcccaaatg atcctggaat aaacaggttc ctgtttgtaa ttgttccatc atttctaaat
     5101 tggctgaatt cacttccata tcaaagaagg acaatagttt ctctcgtgct tctttttgtt
     5161 tcggacgaaa cgcaaatttg taagaaatta actctttttt atcttcttga tcgtaatctt
     5221 taaacgcttg cacaacaagg taaatatccg tattattcgc acgtcctttt cgcaagctat
     5281 catcaatcaa atagcttcct tgtgcggtat cttcataccc ttttgcctcg tcataaataa
     5341 tcgcagcgtc ttcttctttg tttgtagaaa agatatacgt aaacttcata atgacttcca
     5401 taattgccat tcctgcaagt tgttcgctat ttaatcgacc cgaatcaatc tctttttgtg
     5461 tcggaatgcg tattccttgt gtccctaaca cgttgatttg tgaatcaaaa cgtatcggcg
     5521 tactgtgctc attcccaatg agaatctttc ctaaaccaac gctatgccct aaaatcaatt
     5581 tagctagttc tttgtcactt tttcgaattt cttcgataac ttgacttaac gaatgcttac
     5641 ctggcatggt gtaaactaca tcgatggcgt cttggatcac cgttttcttg tggctcgctg
     5701 tttctcgatt ggtatctact tctcctaaca ttcctaacac atttttagcg gtttgaattg
     5761 cctcttcttt tggtaaaaac aatagaggat caagcatccc tcgacagctt tcatctgaag
     5821 ataacgaaat aaaattaatt cgttcataca tagccacaaa ctctggtatg tgtccgaatt
     5881 tttcaagtgc ctttaaaaag aacttttggg tttcattttt cggatcaata taaagaattt
     5941 tttgacctaa aaacatcagc cataaaaaaa tatattttac aaggaccgat tttcctttac
     6001 ctggtggacc aataatcaac gtatttccat tggtatgttc cgcgccttca atacttttct
     6061 ttgttaaatt gggataatac catactagtt tactagatag cgctaacgct tctttcgcag
     6121 atttaaactt ttttgcaata acaatccgtc ctaaaggaaa tcctttctta tttcctactt
     6181 ctttctttaa gtccaagcca taatctgcca caaaacctgt cgtagcgacc tgctcgtaac
     6241 tacgaaaacg ttgcgtactt ccaattaagc attgattaaa caacgttagc tgatccgcta
     6301 ccgatcgata aaatttgaaa tctgtagact caatcgcaaa ttcaatatct ttgactcgtt
     6361 cttccaattc ttcttttgaa gaagcagata caaccagcgt gacggtagaa taacacaatc
     6421 gtttttcttt gcgcttcagc ttatctttta catccattaa ccgttctgta ccaaaattta
     6481 atacatcgtc tgaatctaaa atgccgtcaa cggtctcata ttctttttct tgttcataaa
     6541 tccgtccagc cattttatta atttttcgtt catcagattt tgaatgctca aatcgaagtc
     6601 gaatatgcgt atcaattgga aaagataagc tatcctgaag attttgaata aagctactcc
     6661 catacatggc taaaggagcg tccgtttgac ttaaaaacgt cgaataatgc gtttgattca
     6721 actgttcaat cgcaatgtat cctgcttcat tcgaaatgac gccctcgcaa atttccattg
     6781 gcgtaagctg tctttcaggc aatttttctt ctgatcgatg gaaaaaataa taataaattt
     6841 gtccaatctc ttgggcagat aagcgctcta tcggcttata attggacaat tccgtataga
     6901 gctccttttc taatgtttca tacgttggta acacactact tataggaact cgttgtcccg
     6961 taatacgata gataaacttt ttaaaaacat gcttaagcaa ttgaaaatat tcgatcggat
     7021 cagacacttt catttctgta gtgaaacgca caaacagata cgttcgatac tcgtttaatt
     7081 gcacttcgtc ttttaaaatc tttcctgcct gttcaaagta cactttccca agatctgcgc
     7141 attctccctt taccaaatct cgaatggttt cttcaatgtg cgtatctagg ttaaattgac
     7201 gtggaatatt gataaaatga tagtcatatt catcatgtcc aagtatgcct tcgccatctt
     7261 gaagatagtt cttgaaaaaa tcaatgtcat ttaaaggaaa tacttgggaa gaaagttgat
     7321 agcctaccca cacatcatca tttttggtca aaacaaaatt tcggataatg gcgtctatcg
     7381 gaaattctaa tcgaggaagc ttcgtttttt tctttttgaa ctgccaaatc ccgataataa
     7441 agacgaccaa aagaaacaaa aacgccattt actcgactca ctccttccat ttgtgtaggg
     7501 aatacgtcac tttttcatgg aaaaacggca catacatttc atgttcaaag cgttgtttcc
     7561 gtttctgata ataggacgtt cgcccaatta aatactcgaa aaagtgttta ttgtcccatt
     7621 tcattgaaaa caatgtccaa acgagaacac caatcccaaa aatcataatc aaccaggact
     7681 ttcgaaacag tgtctgcaag aaactaattt gattcacata cgcaatcaaa aataggatca
     7741 aaagaatgaa gagaaatacc ccacctatga ctaattgttc catgcgaatg ccattagaaa
     7801 tccagggaat tttttgtcct ttaatcccgt aaattttgtg agggtgctta aactcctttg
     7861 tatagttgta ttccatgttc actctacctc cttttacaaa aaaagaagcg accagaagtt
     7921 ctaatcttct gatcgctttt gcagtttgtc tttttcttat gataattgta tccagggcac
     7981 aatcatttgc gttaccccca cgttctcttc actatcctta ataaagataa tgttctttag
     8041 ccattcacca atatcaacgg cattcgataa caataagacg agcattatca aaataaagac
     8101 gccaataatg gcaaacgctg cccgcgcaaa gccttgggtc gcataagcac caaccattag
     8161 aacaatcgca acgattgcca atagccaaat gacttgtcca gatacatcat caaataacga
     8221 tttaagatca aaaagtttca tttccattct cctctaagtt cattttattc atttaacgta
     8281 gtaacaaaat aactatcgcc acttgttttt acttccattg aaaagtttga ttggaactcg
     8341 tttcctttct caaaatagaa acgatacttc ccttgcaaaa agtagtttcc ttctttattt
     8401 tttactgcat gatccagttg tacattgatc aattcgccgc cttctaaccc tttttcgtta
     8461 gaaatcaatg ccaatttttc atcatttttg acatataact caaaaaaccg tttcacaaat
     8521 gcttccagct tgatttcttc gctttcagtg accgcttgcc cagaagaata aaatcgattt
     8581 tcctgatagt tcacagaatt ggtattctta ctctcagaaa gattcaaaaa ttgaacgcca
     8641 tcaagtactt ttaaaacatt gttttgataa ctgactggaa aagaaagttg actgcttttc
     8701 caatgttctc cttctgtaaa agtaacctca taaaataggc gatacattgc ttgtgtcaaa
     8761 tcgatttctt ctagtttaat aaagcgaaca tctttcgcta ctcgattggt agccgtcgtt
     8821 acaaagccta gatcagacgc ttccaatcct tgtgctaaat aatgattgac ggaagcctcc
     8881 cattcgtttc gtttttctgg cgaataagta aataaccttt caataaaatg ttgtccctcg
     8941 tacttcaatt gttccgtaga agacaaggtt tggttgactt cagttaacaa tgtttctgga
     9001 ttgacttgtt ttttcactgc taaacgcgtt aatgtatcca ctcgtccaaa gtttaggagg
     9061 agcacgacac ccattaacag aaacaaacaa aaaaaagcgc ctgaaaatac actcatggca
     9121 cttttttgcg aaaattggat cattcgatct gacggttctg ttttcttttt ttggaacaaa
     9181 aaacgtttct tttgtttctt ttccttctgt tgtttcggtg gcttagtttg gaacgcttgt
     9241 ggattaaatt ttgcattact catgagaaat gccataccgt tctaacaata atttcggtat
     9301 tttgtccaca atcactactt cgtggacaac cacacttgcc ccctc
//
LOCUS       DAYYDS010000096         9270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000096 DAYYDS010000000
VERSION     DAYYDS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.099"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACHA02_002385"
     CDS             complement(<1..281)
                     /locus_tag="ACHA02_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8670686.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFS"
     gene            482..1696
                     /locus_tag="ACHA02_002386"
                     /pseudo
     CDS             482..1696
                     /locus_tag="ACHA02_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1875..2210)
                     /locus_tag="ACHA02_002387"
     CDS             complement(1875..2210)
                     /locus_tag="ACHA02_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8670687.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(2194..2457)
                     /locus_tag="ACHA02_002388"
     CDS             complement(2194..2457)
                     /locus_tag="ACHA02_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8670688.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2917..3720)
                     /locus_tag="ACHA02_002389"
     CDS             complement(2917..3720)
                     /locus_tag="ACHA02_002389"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8670689.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3698..4822)
                     /locus_tag="ACHA02_002390"
     CDS             complement(3698..4822)
                     /locus_tag="ACHA02_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8670690.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4838..5653)
                     /locus_tag="ACHA02_002391"
     CDS             complement(4838..5653)
                     /locus_tag="ACHA02_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8670691.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5643..6515)
                     /locus_tag="ACHA02_002392"
     CDS             complement(5643..6515)
                     /locus_tag="ACHA02_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8670692.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6600..7889)
                     /locus_tag="ACHA02_002393"
     CDS             complement(6600..7889)
                     /locus_tag="ACHA02_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8670693.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8033..8545)
                     /locus_tag="ACHA02_002394"
     CDS             complement(8033..8545)
                     /locus_tag="ACHA02_002394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670694.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8612..8755)
                     /locus_tag="ACHA02_002395"
     CDS             complement(8612..8755)
                     /locus_tag="ACHA02_002395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8670695.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2876 a         1956 c         1485 g         2953 t
ORIGIN      
        1 gaaaaggtcc ttccacaatc atggcagaga aaacgggtac gttttaattc taaaatagtt
       61 aacttttctt tgactttcaa tatctgagtg cgtgtcgtat aggaaccgtt gcggataata
      121 cgaaccgatc gacagtgttc acaataggaa ggagagtcat ccaattttcc tttaataaac
      181 tgcgcttgaa cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt
      241 ttatctgtta atccgagtaa ttttctagta tgattatcca ttgagagact cctttggtag
      301 gatgacgttt tggtcgacta aattttacca aaagtgagtc tctttttgag ttgaaacgaa
      361 gaaaaagtgt tagtagaaaa tcgccttcga ttttccacca acacaaaata ttatagaagc
      421 aaaatcaatt ctaccttaga tgttcttata tcactctatc agtcctattt gacaaagagc
      481 caagaaaaag acgttggtcg tcgatgctgt tctctcgcct actcctcgtg cttgtagaag
      541 ttgtggttct actgtggtag atggaaacgg gaaagcaatt atagtgaaaa atgggaagaa
      601 ggaaacgatt gtccgttttg aacaatacaa tcatatgcct ttggttacgc gcctaaaaaa
      661 gcaacgctat acctgtaaaa actgtcgtac ccattggacg gctcaaagtt attttgtccg
      721 actcagccat tcaatcgcag atcatgttcg atataaaatt gcttctttac tgactgaaaa
      781 agtatctttg tcttttattg cgaaaagttg tcaggtatct ttgactaccg ttactcgtac
      841 attgaaagag tttaaaagct atttaccaaa gccttctaag aagattctcc ccagagtatt
      901 gatggttgat gaatttcgtt cgcacgcttc catagaagat aaaatgagct ttatttgcgc
      961 agatggtgaa acaggaaact taattgctgt cttgcctacg cgtaaattac ctcgattaac
     1021 aagctatttc ttaggctgca ccaatccaga agaagtagaa ttcttagtca cagacatgaa
     1081 cgccgcttac ttccagctta ccaaacgtgt tctgccaaat gcgaaagtag tgattgatcg
     1141 gtttcatatt gtcaaacaca tgaatcaggc actcaatgag ttgcgtatcc gtgaaatgaa
     1201 tgagcttcgt aaagcaggac agaaaagtca ggcagaaaaa ctgaaaaaga actggcgctt
     1261 tctgctaaaa aatcgtgcaa gcatcaacca ttatgagtac aaaacatgga aaagtttccg
     1321 agcaccaaaa tacccatttc tcactgaagc aatgatgatt gatcgattgc ttgagttttc
     1381 tgcgcctcta aaggaggcgt atcccttttt tcatgaatta gtcgaagcct ttcgagacaa
     1441 agaccctgac ttatttttct ccttattggc agaacttccc gaaacgttag atgacggctt
     1501 tcgggagaag cttcaaaacc ttctgaccta tgaagaaggt atcaccagcg caatgatcta
     1561 tccttattcc aatggaaaaa ttgaagcgaa gaatacccac ataaagacaa tgaaacgagt
     1621 atcctacgga tttaaatcat tcgagaacat gagaattaga atctttttga tcaatcaatt
     1681 aatcaatgta agataacaaa aaatctgaac cagaatgtgg ttcattctga cccggatttg
     1741 attttgcaca actcatcagt ccttattgac aaagagccaa aaatctgagc cagaatgagg
     1801 ttcattctga cccagatttg attttgtaca acccatcagt ccttattgac aaagagccct
     1861 tctttttagt attattataa ttcttccatg cgttcttcca gtcgctgtaa atcgcttgga
     1921 cttaattccc caattttttt ccatttgacc tttttgttgt ttttgggtaa ttcgtttaga
     1981 gttcctacgt caatccaaga ttcttttttt agtcctgcct gtttccaatc ccttatttca
     2041 acgtatttca gctttatttt atcggattta gtatgatatt ttgaagtaat tgacagaaac
     2101 gaaattgtgt ccagtccatt ttttagaata tagacaggcc ggcgtttacc gccctctgca
     2161 gctgtgaaag caacgtagac ggttgctata tcattagtat tcatcttcat tgaaccattc
     2221 ttcaattttt tcgtcagtgt ctagtttttg gacaggcaat ttttccgcta atctttgaat
     2281 attatggatt cgtttacttt tttccgacaa ttcaatagaa aatgggattt ttccttgctc
     2341 aacaatttgc ttataaaaaa cggtgacggc tgttttgggg ctcatgccta gttcatcaat
     2401 aatgctttct gcttcatttt tcaatttttc gtccatagaa atttgtaact tagccatttt
     2461 ctccacctct ttctgacaaa cacattatat cacggaaaga gttcaaatgg aattaataca
     2521 actacttttg aactccttga aatgcagtgt atttatctaa atataaggaa aggatatagt
     2581 gctaaatggc cttgatagat agaaaagttt caaaatccgc cacagcgata attgaagcga
     2641 acactcaacg acacacagaa caggaaaaga ctcctaaaag aaccaaatta actaggacgt
     2701 gtctgaaaac tccacaaaga tgtggtatgc tctggtaaaa aacgaatggc acaaccacga
     2761 agaccgatat ggttcttgga aaactttctc cacccttttt gtaattggag ggatagtggt
     2821 ttattgatag cgattttcca agttcttcaa gtggaaccag acattgagca ccacggctaa
     2881 aaagtgaatg aagtctttat ttttaatcaa taatttttaa tgattaccag taattggttt
     2941 tttcatgcgc cctatttcta tttgatgtgg agttaaataa tgctgataag taaaaatttt
     3001 tccgtcaatt aaactgccac tagtgaatcc tgttctattg gtaaaaagag aatagtctgg
     3061 gtattcatct acaccaaatg ataaagattc agccataaac tgttttttac cagcaaattc
     3121 agcactgcta ccttgatgga tatgtcctac aaaaatacct aaaatatcac tttcggcaat
     3181 caattcagca aaaccttccg gaacttcagt tacaatagat ggatcttccc acgacaatgg
     3241 atgatgaacc aataaaatcg ttcccttttc agcaggtgta cccaaccact cttgtaattg
     3301 aatcatctgt cctatcgata tttttccttc gtgctgggta tcttgagcag aatccaagga
     3361 aataattcgt aacccatttt gattcgtaca atggatatac tctcccgttt cattgctttc
     3421 aacactcatt ccttcaaaga aaacactgcg acgatcatga ttcccacgac aaaaatagta
     3481 aggtgtggtt gggaaatatt gatccaacat tttccgtaag acttgataat cagaaatccc
     3541 cccttcatgt actaaatcac ctgttaatag aataaaatcg tacactgtta acgagagttc
     3601 ttgtaacaat ttttccaatg ccaactgtgg atttttaatt gttggaaata gttcagcagt
     3661 aaacaagtta gtttgatagt tttctctaaa atggatatca gagagatgca aaaagcgcat
     3721 ggctattccc tctttcttca ataaaataat caggatatcc taaattttct tgcgtctgat
     3781 aatcaaaaaa atgcattctt tcaggttcta tgcggagatt gactgcatca ccaatctgcc
     3841 aagtaggttt ttcattaagg acaatcattt ctataccatc gactttaacg gtgtacgcat
     3901 aactttgacc taatagctca cgatacttca cttgaccgtg taaatcgctt gtttcttttg
     3961 aaaaggttac gtgttctggt cttatgccta aatacaatga gccactaacc tgaagaagtt
     4021 gtttttgcca agctggattc aatgttaaaa attgactggc tacctttaat gttccttgat
     4081 cataagtaac tggcataata ttcatgcctg gcgaaccaat aaaatttgca gtaaaagtat
     4141 ttttaggttt cgtataaaca tttttcggcg tatcaatcat ctgaattcta ccttcattca
     4201 ataaaacaat ccggtcagct aacgtcatcg cttcaatttg atcatgagtg acgtagacaa
     4261 aagtttgttt ataacgttga tgaattttta ccaattcttt tcgtgcatcc aatcgtaaac
     4321 gcacatccaa atttgataac ggttcatcta ataaaaaata attactccgt ttaaccaccg
     4381 cacgagccaa tgctacacgc tgccgttgcc ctcctgacaa atctttagga agtcgatctt
     4441 ggtatggcgt taaacctaac atcttcaaag cttcttccaa acggtgctga atgtcagctg
     4501 tcttaatttt attcgccctt aatgcaaaaa taatattctc ctttacagtc atgtgaggat
     4561 ataaagcata attttgaaaa accattgcga tctctcgttc tccacaaggt agcttatttg
     4621 ctagttgtgt acctaaaaaa agctctccac tcgtaatttt ttccaaacca gcaatcattc
     4681 tcagcaaagt agattttcct gaaccagatg gtcccagtaa cacaattcgt tctccagcgg
     4741 ccacagctaa atcaatttct tttaaaatgg tatgttctcc atattttttt ccgatatttc
     4801 ttaattcaag cgtttctccc atgatttgtc ctccaattta tttaatacct gcagaaacaa
     4861 aagtcccgat aatatatttt tgaaaaattg catacaagac taacggtaca aacattgtaa
     4921 ttaaactaat cgccattgct acggtaaaat ttgtcccacc ttcagaacta atataggtct
     4981 gcatcgccaa cggcaaagta taattatcta ctgtccgtaa aattaatacc ggccaaacat
     5041 actcattcca agaggtaata aaaaaccaaa taccggtaga agtaatcgca tgtctagtaa
     5101 gaggaaaaac aattttactc atgacggttg tttgtttgat gttatccaat gccgccacat
     5161 ctagaaggct cactggaatt tcccgcattg tttgcgtcaa taaaaaaatg cccatcgcat
     5221 cagcaaattg tggtattatc acaccccaag tagtatcaat taaattaagt tttgcaatca
     5281 acaaataatt aggaatcatc gcaacagtaa aaggaatgaa catcgtacca atgatgataa
     5341 agtaccagat tttcttccct ttgattcggc aataagtaaa actatatgcc gcaaaaaaag
     5401 ctacgcctaa cttaacaacc gttataattg tagcaataaa aaaagtattt ctcgtaattt
     5461 tgataattgg aagattttcg aacaaatagc aaaaattttc caaggtaaac ggctgtggta
     5521 tcaaactagt aatcgtctga taggcatctt tcaatggctt aaaagaattg gccataataa
     5581 acaataatgg atagataata atgattatga tgaacataaa taagagatgt ggcaaccagc
     5641 gtttaatttt catagtaaac ccccttttcc acaaatttaa atattacccc taagaaaaca
     5701 gcaaatatta ataaagttaa aactgaaatt gctgaagatt ctccgacttt atacatttca
     5761 aaagcctttt gataagcata atagatcacg tttgcactac cattattcgg tccaccacga
     5821 gtgatgatct taatcggagt aaacacatac tgcagacctt gaacaatggt catcaccaat
     5881 aaatatagtc ctgtcgaact actcataggc aacacaattg taaaaaaaat gcgccagatg
     5941 ggtacacgat ccaacttagc tgcttcgatc atctcttgat caattcccac aattgctgca
     6001 aaaatgacaa tgaaattata accaaaaact ttccaagcag taatacctgc aatcacagca
     6061 attacccaac cttgtaaatt catccacata ggcagtctta aaccaaaatt tcccaacaag
     6121 acagcaattg gtcccgatac cggatttaat atccatgtaa aaaccatccc cgccactacc
     6181 aaagaaatca cagctggaat aaaaaataat ggtttataca aattctgccc tttttctata
     6241 actattccta ataaaaagga tgtaatatat gggaacacac agctcagcaa gacaaataga
     6301 acggtatagg acaaagtatt cttcaacact ttatgaaaga ctggatcagt cagcaccttt
     6361 acatagttgg caaagccaac aaattcttta gtgggggcca ccatattcca gtcaaaaaaa
     6421 ctcaaataga tcgtataaat caatggataa aaaacaaaca cgcccaacaa aataattgct
     6481 ggcgccagat acaaataacc tacatagcgc ttcatttccg acagcctcct catccttatg
     6541 tcaaaagcag acgaccttgt ttttcctttc ttacaggcgc tgtcgtctgc ttgctttgct
     6601 tatttgagta actcattaat ttccttttga gtagctttca tcgtttcatt gacggaatta
     6661 cctgataaaa ttttatcccg catatccaac aatttttgtt cagcttctaa tccagcatct
     6721 ccaggaaatg aactccaact gaccatctta tccatctgat caattgctgg tatcatcatc
     6781 tgattttcag ctaagaaagc ttttaatcca ttttgggcgt ctgcaacatc tttacgtggt
     6841 ggtaaataac cagtaccttc tgtccattta gctaaagatt caacactata aagatatttt
     6901 aagaaatccc aagttgcttc ctttttctta tccgtttgtg atgtaacagc taacattgcg
     6961 ccaccagcag gtagtttaac ttttttccct tcccaacttg gtgactcaac tgctttagca
     7021 gtaaaagtac tatttttttg aatgttgtcc ctttgagcaa tagtggtata gaccatcgcg
     7081 acgttaccat caataaagct ttgcacacct tgttcccacg tagtgtgtaa tgccgtctgg
     7141 tcttttacca tcctttgata taattcataa gcagcgatac catctttaga agcaaaggta
     7201 gctttgccat cttttattac acttgcccca ttactttcta aaattgcttg ttgtgcccaa
     7261 ttatcagcag gttcttgaat atacacgcca tactttccag ttttttcttt tattatctta
     7321 gaaaaagttt gaacttcttc ccaagttttt ggaccatttt catccaagcc cgctgctttt
     7381 aatatatctt gatttaaata taaaaccgga ttactaatgg agtaaggaat acccacttgt
     7441 ttaccatctt tttcagacag tgccatgata ttgtctaaaa aatgatcttt aaaataatca
     7501 gcattatccg gatccacttc ttctactaaa gattgaggat tagtatattt gaaattttct
     7561 gcaaaataat ctttaaaagc ccatcccact tgtactacgt ccggtatttc tttggaggca
     7621 acagaagctt gtaaattttg catcaagcct ttatacatat caggattata ttttgctaca
     7681 accttcactt tgtcttgaga ttgattgtat ttatctacta attcattcac tgtttttcca
     7741 cctagggtat ccgcattaac atgccaatac tctatcgtta cacgaccctc cgattttttt
     7801 gttgtcttgg tattcccact accacaagct gccaaagaga tgacacttaa aacagacaca
     7861 acccctataa caagattttt ctttaacata acattaactt cctcctaatt tctttatggc
     7921 catttttcta atcaagaaat attatgaact accaatgtaa atttctagtt aattttctgt
     7981 aaataattcg aaatttgtgc atttcggggt acagggtcac tggacatatc catcaagcta
     8041 agaataaagc ccccatatgg tcatgtatcc catctccaaa tctcttaact cctctgtttt
     8101 gattataaaa tagtttattc tgtgctcctt tcttccataa ataggcccat gcacaacgtg
     8161 aggttatatt ataaacacca cagcgcgcaa ggtactcaat cgtttctagt tcattgactg
     8221 tccatacaga aaatgtcaat cctagctgct gccccacttc acgggccaac ttagttaacc
     8281 atccttcatg aatattgacg atttgaacgc tttgataggc taatttctgt aaggtatcga
     8341 tcaaatctgc ttcggtaata tccccaagtt ttttcccttc gaactgattt tcaatattat
     8401 aaaatatatg ctttccccaa atcgcagccc aagataattg tacatagcca ctaagaacaa
     8461 tttgtggcca gatttgatag gtttgtgcca gcgaaaaaac ggccatttcc aatccctctt
     8521 cttttaaatc acagttaatg gtcatggttg ccttctcatt ggccactgct tgaaacacct
     8581 gctcaagggt taccgaatct gggacaactt cttacctgtc atgggctagt attagcgtac
     8641 catctgtttg ctgacgcaca tctacttcaa aacaaagctg gcgatccaaa caaaagcgaa
     8701 gaaatgccca gctattctcc tccgtattgt cactaccaga gtgtgcagtg atcatggtca
     8761 ttttgacccc tcctttaatt ccgtgatttt ctcccacacg tccactatac cgatgaagcg
     8821 ttaatttctt gttaatttct tgtaaattcc ctgcgttttt tcagtaaact agttgtaaat
     8881 cttgcatgct ccttcctacg aactcatgaa tcgtcgattt tgcccttcta gctagtttca
     8941 ccaattaagt agttctacat tccccgcttt taacctacta cacctttttt tgttttcagc
     9001 tattccttct tcttgctgaa agtccttgag gttctttctt acaggatacc atcaaggtgt
     9061 tcaatggctt attcccattg aatgaccgag acttactatc agatttctgc tcgttgaacc
     9121 aagtctttga tacaggattt cttccgtcat cttctcacaa ataccacctt gcgtttctct
     9181 agcgattggt cgatgtgcac cctcccagtg gactgtcact accccttttt gctatgccta
     9241 gcccacaaaa aaagctgtcc tcacagacag
//
LOCUS       DAYYDS010000097         9197 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000097 DAYYDS010000000
VERSION     DAYYDS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.100"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(598..954)
                     /locus_tag="ACHA02_002396"
     CDS             complement(598..954)
                     /locus_tag="ACHA02_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8670696.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(968..1378)
                     /locus_tag="ACHA02_002397"
     CDS             complement(968..1378)
                     /locus_tag="ACHA02_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8670697.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1725..3911)
                     /locus_tag="ACHA02_002398"
     CDS             complement(1725..3911)
                     /locus_tag="ACHA02_002398"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8670698.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4129..4812
                     /locus_tag="ACHA02_002399"
     CDS             4129..4812
                     /locus_tag="ACHA02_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8670699.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4893..5612)
                     /locus_tag="ACHA02_002400"
     CDS             complement(4893..5612)
                     /locus_tag="ACHA02_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8670700.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5924..6130)
                     /locus_tag="ACHA02_002401"
     CDS             complement(5924..6130)
                     /locus_tag="ACHA02_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670701.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6375..6977
                     /locus_tag="ACHA02_002402"
     CDS             6375..6977
                     /locus_tag="ACHA02_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8670702.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7118..8398
                     /locus_tag="ACHA02_002403"
     CDS             7118..8398
                     /locus_tag="ACHA02_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8670703.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8411..9139
                     /locus_tag="ACHA02_002404"
     CDS             8411..9139
                     /locus_tag="ACHA02_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8670704.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2657 a         1719 c         1510 g         3311 t
ORIGIN      
        1 tcttgaattg aatcaagacg ccctacacat ttggtttgtc ttactttgtt cagttttcaa
       61 aggtctactt catcgggagt gtttcccgat tcgtttttgg ctgccgtagc aacttttata
      121 tcatatcaga gagacaagcg attgtcaact cttttttaaa agtttttttc gatttcgttt
      181 ttcgaaatct ttcagcaact catttatcat aacttatcgt ttgttatttg tcaagaactt
      241 ttttgaagtt tttttcgata gaatcaagct tcgcaagtgg ttctttttta acaactcagt
      301 tatcttatca tgtcatttct aacatgtcaa caactatttt atatgttttt taaaatacta
      361 ttttattgct ttgctactta aaacaacaga aatgaatata tcatgtaaat ttacacatgt
      421 caatatattt ttaactctat atccccacct agatttgaat tttgaccaca taaattgagc
      481 atgtctctat agtagaaaaa taaagtcaaa aaaagaacac ataacacgct tgatgctaag
      541 tgttcgactg ttacaactgt atgaaatgct cagtgacttg attaaaacag ttaaagtcta
      601 tttatttaag ataagctgtt ccatcaactg ctcattgtat ccaataaata gaacttcatc
      661 gataataatc aaaggacgtc tgaggagatt ggtatgttgt gtgcataaaa aaatcagttg
      721 tttttttcct acacaggaaa ggtcaatcgc aatgttgtga aaagcctcag accaagtcgc
      781 aaatagctgt tctgcttgca cttcttcaaa actgcataat tcttctgctt cttctgtact
      841 tagatcgcgt tcttctaagt caacaaattg ataaggcaat tgatggtgtt ctaaccattg
      901 tttcgccttc agacttgtta cacagtgcgc tgatccatag actttcactt ccacgaaggc
      961 tccctcccta tgaatttaag atgttattgt tttgtaaata gatcagattt ctggcaacac
     1021 atcgaatttc acgagggaca aatgaccgga tctcatcttc attaaatccg attagtattc
     1081 ttttttcatc aatcaaaata ggtcgtctca ataatgaagg atattctttg aataagacga
     1141 tcatttgctt taaagttaat tcgttaaaat ctatgcccag cttttcgtaa attttcgacc
     1201 gagtggaaat aatatcttga actcctcctt cagtcgctgc caagatcttt ttgatctcac
     1261 tttctgttaa cggttcggca aaaatattct tctcttcaaa ttcaagttga ttcgcttgca
     1321 accatgctct tgcttttcta caggaagtac agctaggcga agtatatact tttatcattt
     1381 tctcactcat ttttaattat aatccaattc tacagtaatt tcttttaaaa taccaattat
     1441 tagctaataa tatataaaaa aaaatcaaaa taactgatta tttacgtttg aataatgaga
     1501 atttttctat atattctcta ttttgataat agtattctca ttttcaaaat aaataaggga
     1561 ggtgtgacaa aattcttgtc acacctccct tttccgaata aactgtgttc cagaaatagc
     1621 ttcttcgaaa ataagctgaa atttcacaaa aatttgagaa gcaattttcg tacattcctt
     1681 cttctttctt gaaactgacc acttctgtca caccctctct tctatcactt tccttttttc
     1741 acacgactcc cggtttcttt gataaatctc gatactttca attttcttga atttttcatt
     1801 tgaggtacac atagatacag acgatttctt gctctagtca tcgcaacata aaatagtctt
     1861 cgttcttctt ctatcgtcgt cactggttct gcttttattt cattaaagtc gattactttc
     1921 ttttctgtcg ctccgttata aggcaaactt ccttcgttac aaccaatcaa aaagacattt
     1981 cggtaactaa gacctttact tttgtgaatg ctctggatac atagtgcatc aagttttcct
     2041 tgtctgtaag caatcaagcg ggtttttaat tctttagctg tcgcttgggt ttcttgcgat
     2101 aaggcttgga gtcttctgaa agtagcatgc tgctttactt cttctcgtat ccattcttga
     2161 tcactctggt cgatttcctt agctatacgt ggcagatgaa gatagctttg aacaagcgaa
     2221 tagatttctt gatgctggtc tgttttacgc aacgcttcct caaactctcg gatttctgca
     2281 gatttcgatt ttaaattggg gcttatcgac aaaatataat ctgcaacggc tatattttga
     2341 tatttaggag aaaagtattg atcgcattca ctttttttca ggtagcagtc tgccatcagc
     2401 tgccggagca attgctggta ggcctgattt ttcttttgca gattcagtgc tgtttgccaa
     2461 tgaacaattt tttgatagaa atgttggaaa gacttgatac caaagatacc ttcatatgga
     2521 ctatctgctt ccagtacaaa aggaatattc gttttcagca gagattggac aagctgctgc
     2581 gtttgtgaag ctgatcggta taatatgatt gattccttat atttcccatt tttttgaata
     2641 aatgtctgaa tttgtttgat tagccactga ctttctgctt tagtatcaga aaacaaatag
     2701 gcttccataa cacctgcttc tttttgtgct ggaatcatcg cctttggtat ccgatcattt
     2761 ttattatagc tgatcaatct atttgctcct tggacaatag ctgcccgaga acgataatta
     2821 gtcaataaga aaagagtttt ggtaggatat tctttttcaa agttcttgat gtatgctggc
     2881 tctgatcctc tgaatccata gatactctga tcatcatctc caacaacaat caacttgatc
     2941 ctgttttcct ttgtcatccg tttcacgatt tcccattgaa gtggattagt atcttgaaat
     3001 tcatcaatga atagataatc atatttttgg ccgatataat gctggatctc ctcattctcc
     3061 aaagcgcttt tcagattttg gagtacatca ttgaactcta gcaattgctg gcttcttttt
     3121 tgttcttgga gttccgtgag caatccatac agtcgttctg atatcggctg tcgtgcattc
     3181 ttatcaatgg aatcatattt ttctttaact aactgaacca tttcttctac tgaaaggttc
     3241 atattctgcc aatagtctaa ccgagaagca atcgtttttt ctccatattg atcaaatata
     3301 taaagattat atttacgtat cagctgttgc aaaacagcat tcactttttg tgcagtatcc
     3361 tttgaaaagt ctccttgtat tccttgcatt gctacttcag gaaattttcg acaaatttca
     3421 ttgtatatcc taaaaaagat actatggaat gttccgatat ggacagcggt ttttctctgg
     3481 ctgaaattat ctactcgttt ttttagttca tttgcagcgt tccttgtaaa agtgatcatt
     3541 agtatgcgtt cttcgtctac ttgtttttgc gtcatcagat aaacagcttt tgcacagatt
     3601 gtttgcgttt tgccgctccc agctcctgct atgattcgaa gatgtttctt catatcatat
     3661 gtgatagctg ctttttgttc ttgattgaag cgattatagg aacgtttctc catcatttta
     3721 aagaaagagc tgtttccttc actttctttt acttgtactt cttcctcaat aagtccgggt
     3781 gcttcgccat aagccgcttc ataggtagca atgatttctt cttgtagccg aaagacttgt
     3841 tgaagctttt tatacttgtt ctcccagcgt ttcaattctt tttgtaatcg gagaatttct
     3901 ttttcattca actgattgat ttccttccca tctttcttct ttgaatttgt ttatttcaag
     3961 ttggttattt aaagtttcgt tgtctatttt ttctttatac taacagacct ttctaaaaat
     4021 gaaaatggga agcgatatga ttttagtttt ctgaaaattt ttcctttttt tcaatcgttt
     4081 atgctaagat aaggaagaca aaaatattca taaaaggtga tgaaaaaaat gcagacttcc
     4141 tttcacgagg ggcgtacaat aggacttttg ctgacattta tcggaggagc catggacagt
     4201 tatacttata tccaatatgg tgcttttgcc tctgctcaaa ctggcaatat cattctatcg
     4261 atcatccaag catttgacgg agaatggcta agtgttggta aaaaagtatt gtctaccctt
     4321 ttctttttct tagggattct tctaacaaag tatctaattg attattttag aaaaaaagaa
     4381 aaacatttct ggcgcttgtt tgttctatat tatgaagcag tgattttttt ctttgttagt
     4441 ttgacacccg tccatgagca tcccgcatta gtaacgattt tgattgcttt tactgcatcg
     4501 atccagtggg tagcttttga taaaatcaac gggcgtgcct acactaattt atttacaact
     4561 ggaaatttaa aaggggtagc aacgaactta tatgattatt gtatttctaa agaaaaagca
     4621 gattttgatc gatttttcca ctatctaagt gtcgttcttg cctttatcgc aggagctatt
     4681 gtccttgttt tctgtcatca tctgtttggc gcccattcga ttttgatcgt ttccatttta
     4741 tttttgatca tggccctcac tcagacattt catttatggc aattttatca tacgaatcgg
     4801 ttcactattt gatctcaatg aatcaaaaaa actaagcaag atgctgctca cagagaaata
     4861 tctgcgagtg agcaatttgc ttagttttta tattatttat ttgtaaagat tccctttgct
     4921 ttcgggttat ttgtagtgta gcggatcgac tgggcatctg cctcttctgt cattttcttt
     4981 gcccgcatct tgatttcttg tacgatttgt tctccacatg ccggatcggc tacgaaatca
     5041 tatttgtccc catcgatcac tattttggga catatatcaa actgttcata ccactcttcg
     5101 tatcgtcgat tgagttcttt atagtaggaa tagagttcag gatcttgttc tagctgttca
     5161 tattcccggc ctcttttttt gattctcgct aacatagttt caaaggatac acgaatataa
     5221 accagtagat ctggtttctt ttgtggcgaa atttcttcta actccttcag catcgtttct
     5281 aaaagacctt catactgctt tacttctact tcagaaacac gccctaaatc tgcattcaaa
     5341 tgaaacagca tactatcttc gtagatggag cggtctagta cgttgtcatc atggctgaaa
     5401 gcatctttga ttgccaaaaa gcgtttgttc aaaaagaaaa tctgtaataa aaaagtatac
     5461 ttttctggat tagaataaaa tagcggcaat acttcattat cttccacgtt ctcataaaaa
     5521 ggttttgttt ctaaatcttg ggcaagcatt tcagttagtg tgcttttccc tgcaccgatc
     5581 gttcctgcaa caatgatcac aattatcggc atccttcctg taataaaatc tgttcttcat
     5641 tttactactt catttataga aataaaagcc ttgactttat ctgctcctta aaagctactt
     5701 ctctaaaagt agccaaaagt aactcctatt catgaaaata aaggctgtga caaaagtctt
     5761 gtcgcagccc ttttttcaaa taaacagtat tctcctgcca acacctcggt cacaaggcaa
     5821 tattcataaa acatgaaaag ctgtttttta acattattcc ttgtaactca gagctaaacg
     5881 ggagactcac aacctcttta tgctctcaac attttatctg tttttattct tctattgtac
     5941 ttgatttgcg tacaactaac tgccagtgca gattatgagc ggtatcattg tcttctactg
     6001 cgcatttcat gacacgtatc atcacactat tttcatagat tttttctatt tttcctttta
     6061 ttggtgattc aaataaatca gatttaaatg catatgtttc gccgacttca aacaattgtt
     6121 cattttccat aagaatccct ccatcacctt ttataaggtc agtaattttt taacataaat
     6181 cactcgaaaa agcacaaact tgtcataaaa agtacttttc gtatcaaaac tgtcactttt
     6241 tttggtaagg ttcttggaaa acgaagattt ttttaggaga tcatgacggt tttgatgtca
     6301 aaaccacttt ttatgaaaca aaagacgttt tttatttttt ttagcataaa atttgtggtg
     6361 gaggtacgta tgagatgaaa gataaagttg tgaacaaatt attagaagcc ttattcagcg
     6421 gagacaaatc ggaagaagat atggtgtatg tccaagtaaa atttgcctta gaagttttat
     6481 tgaataatct tggaaaacta gcggtcgttc tagtcttttc tctggtgaca ggctcatggg
     6541 cggaaacagg aatcaccttt ctttcttata tctgcatccg cagatatgct tatggattgc
     6601 attccgactc tgaattcgtt tgtctgctgt ggacactact ttatttatgg ggcgttccct
     6661 tggtgatgaa acatctgcaa ttgactatct ccttcccaat gatggtcttt ttgttaatca
     6721 gctgcttttt gttactgctt cgctatggat caagaggtac agcaatcaat cccattgaac
     6781 ccgagaaacg tcccccatta ttaaagaagg cgatcagcat gtttctaatt ttttctctca
     6841 ttactttatt tttctcagct tcttactttt ctacctattt gctacttggg attgttttag
     6901 aaatagctac gttgcttcct attacaaact acttgatgaa ttttggagga atctttcatg
     6961 aaaaaaacaa tcattaaaca attaactaac ttatctgact tttttgtatc tggctgctgc
     7021 tttggtatga gttctgaatt caaaatccct gaaaaattaa aaaaataata cgctaattgt
     7081 gctataattt ggtaaaaatc aatgggagct gtactttatg tttttatggt ccatagcgtt
     7141 gcaatatttg atttacacaa tttgtatctt attgattgac catcacatcc gatacaaata
     7201 tctgtttgcg ggagtcattc tgatttcttg tattttgatt ggatgggtct attcgatttt
     7261 agggattttt actgctctaa ttgctatagg aatgctgatg cttacttttt tcttcttttc
     7321 gacttcgcgt caacaattgg tttacgcgct tccttttggg ttattgacgt cgcttttagg
     7381 agatcactta acaagcatcg acgatttttt tgttttgaaa tcttcggtta ttgaaccagg
     7441 agattcattg cagtattttc acattgtctt atccgctttt ttatctgctt tattagcttt
     7501 ttgtttaaaa aaatcgttag agacttggtt tcacactgtg gaccgccgta tgatcggtac
     7561 gatcgggaca ttgatattat ttacatacta tatcattatt ttttataccc gtttttctgg
     7621 tgaaacacct gatgtcttag ctttgaatac gagttttttc attctatatc tgtgtgttgg
     7681 cctgatcatc ttggtttatt attggaaaat ctctaataag aaattagctg atcagttatt
     7741 agagcaacaa gtcaggatgc aacaggatta tatcaaagat ctagaaaaaa actatcaaga
     7801 cttgagagaa ttcaaacatg attaccaaaa tctgcttttt tctcttcaca gctatatttc
     7861 agaaggcgac ttagaaggat tacggtctta ttatgatcaa acgatattac ctacacgcca
     7921 gatgatgaac ctttttccgg ccaatctaag tatgttggat aatatgaaag ttcctgagat
     7981 ccgtagttta ctgtcattaa aactcatgat ggcacaggaa aaagggttga tcgtccagct
     8041 tcattttcct gagccaatcg aactggatac gaaacatacg gtaaaccttg tacgtatgct
     8101 tggtattatc ttggacaatg cgattgaagg agctagtgct gcaaagaaga aaaaaatcga
     8161 attgaccata ctcaagaaga atcattccgt gatgattcgt gtgatcaata caacaacaaa
     8221 tacattaccg ttaaaccagt tgaaacaaaa agggttctca acaaaaacaa atccaaaaaa
     8281 cgaaggcctt ggcttgttta tcttggatga attgacaaag accaatcctt atcttttcct
     8341 tgaaacatca attgaaaacc agcgtttttc tcaaacgatt tatatacaaa taaaataaag
     8401 gagtaatgat atgattccca tttatatttg tgaagacgat ccaaaacaat tagaagtttt
     8461 atcaacggtg atcaaaaata gaatcatgat tgaaaactta gacagttatg tccatatttc
     8521 tacctctgat ccggaagaac ttctcaatgc agccaagatc agaacttctt cttttggtat
     8581 ttacttttta gatatcgaac tagaagacag tgaaatggat ggtgtagcga tcggtaaatt
     8641 gatccgtgaa ttagatcctt taggaaaaat aatctatgtg acttcccata cggaaatgtc
     8701 gatgaaaatc ttaaaaagca acatcgaacc gactgattat atcgtcaaag aagatcttta
     8761 tgatttaaaa gaaaatgtcg agcatatcct gagcaaaatt ttcgaagatt ttcaaatgtc
     8821 ccaaatccaa aaagatatct tcaaaataga gtttaatgat gagatcaaat ttttaccgat
     8881 caaagacatc caatattttt ctacttcgct aggagcgcca cataaactgg aagtccattt
     8941 gactcatgcc caattacaat tctatgagaa gatcaaagaa atcgagaaaa tgcatcggaa
     9001 ttttatccgt tgtcataaat cctatgttat caatacacaa aatgtgcggt cgattaataa
     9061 aaaaaccaga gaagtcacgt tggcaaacgg cgagaccatc ccagcctcta tccgtggatt
     9121 aaagaaattg atttcataaa aagaaccgaa tagtttggac cgactaaatg accagccgat
     9181 ccaaaccctt cggttct
//
LOCUS       DAYYDS010000098         8956 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000098 DAYYDS010000000
VERSION     DAYYDS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.101"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..318
                     /locus_tag="ACHA02_002405"
     CDS             19..318
                     /locus_tag="ACHA02_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670705.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(415..849)
                     /locus_tag="ACHA02_002406"
     CDS             complement(415..849)
                     /locus_tag="ACHA02_002406"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8670706.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1081..1791)
                     /locus_tag="ACHA02_002407"
     CDS             complement(1081..1791)
                     /locus_tag="ACHA02_002407"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8670707.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1781..2641)
                     /locus_tag="ACHA02_002408"
     CDS             complement(1781..2641)
                     /locus_tag="ACHA02_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8670708.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2646..3284)
                     /locus_tag="ACHA02_002409"
     CDS             complement(2646..3284)
                     /locus_tag="ACHA02_002409"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8670709.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3299..3598)
                     /locus_tag="ACHA02_002410"
     CDS             complement(3299..3598)
                     /locus_tag="ACHA02_002410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8670710.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3641..5125)
                     /locus_tag="ACHA02_002411"
     CDS             complement(3641..5125)
                     /locus_tag="ACHA02_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8670711.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5145..5606)
                     /locus_tag="ACHA02_002412"
     CDS             complement(5145..5606)
                     /locus_tag="ACHA02_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8670712.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5624..6691)
                     /gene="ulaG"
                     /locus_tag="ACHA02_002413"
     CDS             complement(5624..6691)
                     /gene="ulaG"
                     /locus_tag="ACHA02_002413"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8670713.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6909..7679
                     /locus_tag="ACHA02_002414"
     CDS             6909..7679
                     /locus_tag="ACHA02_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8670714.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7952..8779)
                     /locus_tag="ACHA02_002415"
     CDS             complement(7952..8779)
                     /locus_tag="ACHA02_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670715.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2729 a         1875 c         1399 g         2953 t
ORIGIN      
        1 atagaaagga aataaaacat ggattatact gttgaatttg acagtgaact acttaagcaa
       61 tttccacaag aagaacattt catgtataaa gaagcaattt cccacaaagt gacagagtat
      121 ttggagcata atttttttcg tataaagcca gtgcgtactc gttcggcaaa aggaatatac
      181 gagatgaaag tcaaaattaa taaaaaaaat tatcgcatag catttgcttt aaaaaaaaat
      241 catgtgttca ttttttatat tagttcgaat ttgcaaaaaa ttgtatttga taaagaggtg
      301 agtaaaaaaa taaaatagct gagcattgga ggatagattg agtgatacta agtctatcct
      361 cttgtttttc taaagaacta agctagaatt ttttcttttg tacgttatta tatgttattt
      421 gtcattacca aaaagcacct tttttcttct agtatctatg acataagaaa taccacccat
      481 ttcctttact tgaacaaatc ctactgattc ataaaatttt tttgttttct cagtatcatc
      541 tgtcaataat tcgatttgga ctggtccata actagtgatc ttctccaaag cttcattcaa
      601 aagcactttg cctattcctt ggcgctgata cctcggatga atgaggatat cttgaataaa
      661 tgcgatcgtc gcaaaatcag tgatccaacg aacgattccg ataatcttcc cttccccatt
      721 acgctttatc agagattcag attgttcaaa tgctctagct aaccttgcag tgtcttttgt
      781 gtaattcgtc caaccaacgc tcttatataa ttcagaaatc tcatcgatca taagggaatc
      841 aatattcatt tttttcttcc tttcttatac aattctccct ctaataatac agttctttga
      901 ctatttaatc tatcacatat aactctcctg taaagattct ctcgattcct taaaattcct
      961 ctttcctttt agaatgcata ctttttgata aataaaaagg ttgtgataaa ttttcttcac
     1021 aacctctgtt tctctctatc aagtgttatc ttttaatttt ctacccttac cctagcatct
     1081 ctattgcccg tagtatgcat actttccatg tttgcgaaaa taatgtttat ctagtaaata
     1141 ttgcgggatt tcacatatat tggggttgat cagctcgttt tctttggcca tcagacaaat
     1201 ttcatctaag atcaaactgt tttctactgc tttcataggt gaatcccccc atgtgaacgg
     1261 tccatgtcca tagacaagta ctccagggac ttcgtttgga tctaattttc tttccttaaa
     1321 cgtctcgaca atcacattcc ctgtatgaac ttcgtaagct tctcttactt cttctttcgt
     1381 taattggcgt gtacaaggaa ccttaccata aaaagcatca gcatgagtcg tgccataagc
     1441 tggcaaatcg cgtcctgctt gcgcccacat gacggagtgc gtggaatgag tatgagtaat
     1501 agcagttata tcttcaaaag tttgatacaa aacaacatgt gtggggagat ctgaagatgg
     1561 ttttaagcta tcttcttcta ttatatttcc cgacaaatct gttaccacca tttgatctgc
     1621 ttgcatgcat tcatatctga caccgcttgg cttaatgacg atgactccta gctcacggtc
     1681 cacttcactc acattgcccc aagtcaattt gactaatccg tatcgcggca atgccagatt
     1741 tgcttcaagg acacgttgct tcattgctgc aattttttct tcacaagaca tcatagccag
     1801 cctccttcaa tttagaactt aagtagtctt ttgcctgctg gatttctctt cgaaagtctt
     1861 ggcttttttc actccacatt tcaatcagaa atgttccatc ataatctaga cgtttcaaat
     1921 gtcttaataa tccaagaaaa tcaacacaac cgctaccaaa aggaacatca cgaaactggc
     1981 ctgaatattc ttttgttaca gggtaagtat cttttaagtg gatagcagtg atataatcga
     2041 tccctttctc aagttctcga gccggattat tttccggcca tgcggacaaa ttgcctaggt
     2101 ctggatatac ttgtagaaaa ggtgaatgaa tctgttcttt gatttctaaa aattttgaaa
     2161 tggagttcat aaatggatca tccatgattt ccacggaaag aatgatgcca tatttagaag
     2221 cttcctttac gctttctttt attccttcga taaagtcttc tctggaattc attgatttct
     2281 cttcataata aacatcgtat ccagctattt ggattacttt gacgcttaga ttatgcgcca
     2341 aatggattgc ttttttcatt agttgtttcg ctgctttttt cttttctgga tcagcagaac
     2401 caaacggaaa tcgccgatgc gcacttaaac aaatcgaatg gatacgtaca ccggagctga
     2461 acatctcttc tctcagctca gctatctttt cttcagacca atctaatcgt gctaaccgct
     2521 catccgtctc atcaatcgac atctcgataa aatcgaagtc catttctttt acaagggaaa
     2581 aacgttcttt ccatgaaata tctttgggta acgctttttc ataaatccca atttgagcca
     2641 tttccttacc cccagatacg aatgatctca tctttgaagt tttgtgcggc cttcatcgga
     2701 tcatttgctg ctgtaatacc acgaccagtg ataaaggtat acacatcgat cccttcaaat
     2761 aagtgtaatg tgtgtggatt taatccacca gttacagaaa cattaaagcc tagttcaatc
     2821 agttttttga tcttcgataa atctttttca ccccaagttt caccagataa cagcgcatca
     2881 cggctttgat ggtagattac ctgccggata ccgatgttat gccaatccat ggcttgttca
     2941 aaagtccaat tgccgtagag ttctacttgc aattcaccta tttctttttg tgctgcctcc
     3001 atagttggaa tcgttgcaca acaaatgact gtcatccagt ctgctcctgc ttttgcaaca
     3061 ttactcgcca ctgttcctcc agcgtctgca catttcgtat cagcgactag ttttttatct
     3121 gggaacatac tgcgaataca gcgaatcgct ttttgtcctt cctgtaagca aagaattgtg
     3181 cccacttcaa taatatctac tacatcacca actttcataa catctcctaa tgcatgttct
     3241 aagtcattgt gatctaacgc aatttgcaaa ttcggcctgc tcatcttagt tccttccttt
     3301 acttgttttt ttggtaccac tttgtttcag caaatttttc actgatttct ttgactgaca
     3361 tcacattttt gataccgata actgtaactc cttttttctc tgcctgagta aacatattca
     3421 aaaaattgac tggcgtaaaa actacatcgt attggccagc tgaacttttt ccctctgaaa
     3481 tggcacaatg atggattttg gtaattggta cgtttagttc tttcattgct ttttccacac
     3541 ttctcatcat catcaagctt gttcctgatc catttgcaca cgatactaaa attctcatct
     3601 taatccctcc attatgagtg ttgtctaatc agattttctt ttatactctc tgcttttctg
     3661 catatgtgtc atagtcttct acaatcaaaa aataaccatc aggatttttc cgatattgta
     3721 actgcggaat agccaataaa ccaatgacaa ctacggcaat tccagcataa cctaggactt
     3781 tcatgattac tgtaaacaat ggccaagctg ttgcccagtc gaacatcccg atataaccac
     3841 cgtatgttga catcccaatc catgtagcga ataatgctga accgcctact tgtatgattc
     3901 ctgaaagaaa cgggaacagg caagcggctt tgaatccacc tcggttattg gcataaactg
     3961 ctataacggc attatcaaag aacaacggaa tgaaaccggc tatgacgatc gttggtgatt
     4021 tcaataaaat cagtaaacca atcatcaaaa actgtcccaa agcaccaaac aagaacccaa
     4081 tcgttaccgc atttggcgaa ccaaagccaa aagtagcagc tacgtcaatt cctggaacag
     4141 ctcctggtaa aatcgtgttt gagattcctt ggaaagattg agttagttca tcgacgaatg
     4201 tccgaacacc taattgcaag atcgccagat ataccgcaaa atttaaagaa gtcgtcataa
     4261 tgtagaataa gaaactctgc ccttcttgca tgaattgcgc ttgaatcaaa taatctttgc
     4321 ctagtacaat cagaatgatc ccaaagaaaa atagcatgag taacgaagta gcaaccatat
     4381 tctcattgaa aatcgacaag aatcctggta atttgatatc ttcaattttt ttgtcttgct
     4441 tgattgatct atttttcttt tcatcccgct ttttcatcca ctcagctaat tttgcaaata
     4501 taaagattcc gaacatttgc tgatgtgcca ctgcaaatcc ggcaccttct gtcaattcct
     4561 gcgtgattcc tactgttaag ttagaagcta ctgtccaata acaacctaaa atcagtccca
     4621 taaaaatcaa tgcttgtatc ctacctagat caggaaagca aaacagtaaa atccaaaaag
     4681 cggtagctgc ttgttgaacc tgaacgtttc cggtagtgaa aacggaacgc agtttcgtat
     4741 atttctgaaa acggactaat aagatattta aaacaaatgc aatcagtaat aaaatcatca
     4801 catctccaaa ggtacggcca aatgtctcca ttaatccagc atccacagca ttttgtccaa
     4861 aataaggatc tgtaaccatg gcatccaaat tgaaccgttc ctttagtcca accaagattg
     4921 gccgaaaatt attgaccaaa ccaccagagc caacagtcaa aataaagtaa ccgaccgttg
     4981 cctttaagaa tccagcgatg cattcatgaa gcgggcgttt cagcaaaata tagcctaata
     5041 acacgataaa accaatcagg tatgctggct gcgtcaagat atttgttgca aaatacgtcc
     5101 aaatgcttaa caaaaattcc cccatctttt ttctcctcta tcaattaatt atattctgcc
     5161 atcacaactt ggtaatcatc gatggagttc gtagagatga gacgctcaac caatccatct
     5221 gtcattaata attcggataa tttttggata ttttctagat gttcttctgg atcttttgct
     5281 gcgagtgtaa aaaacaaact agccgttttc tcctcattct ccccttcact ttcaaaacgg
     5341 attggctgtt tcaacttggt aaatgaaata gctgttccaa aaacaccatc acttttttct
     5401 gaagaatgcg gcattgctac ttgaggtgcg atcacaatat aaggaccgta tttttctaca
     5461 caatggatga tctcctccac atacgtttca tcaataattt ccttatcgat caatacaaga
     5521 cagctttcgg cgatagcttc acgccaatcc gtgatttctt tatctgggta atgaatcaat
     5581 tgatggtcaa aaaagtattt cagcaagaaa ctccccttct ttcttataaa aacgacggaa
     5641 acggtgtatc attttcaatc gagaagacat cttcaaatcc tcgataaaaa ttaaactcaa
     5701 gatcatcttt gtttgtcgga tagatgaatt tcccgcctac ttgccagata taaggtttaa
     5761 atccatactt caggcgatcc tttttgaatt tccaaatttc agtgatctct tttggatcag
     5821 ccataaaatt agaccaaata tcgtagtgga caggaatcac tacttttgca ttcaatgact
     5881 ccgccatgcg cagcatatca acagaagtaa ctttgtctgt tatccctcgt gggttttcac
     5941 cataggcccc taaacagaca tcgattttgt gttcattacc atgtttggca aataaattgg
     6001 agtaatgtga atctcctgca tgataaagat tccctcctga tgtttcgaat aaatagttta
     6061 ctgcaatttg atccatctcc tgaggcattt ttccttttaa tacctcttct ggatcttcac
     6121 aagtgaccaa cgctgtccga tcaaacgctt ctaaagctac tacattgatg tctttgattt
     6181 tgactgcatc acctggcttt actgtaatcg ttttttcttc aggaattccc catttttgcc
     6241 acgtgcgaac cacttctttt ggcccaatga atgttgcttc aggacaattt tgatgaactg
     6301 ctgcggctgt atggatatct agatgatcgg aatggatatg tgtaacgatt aaagcgtcta
     6361 cattttttat cgcaaaagga tcgattacga aaggctgagt acgtaaatta ggctgcatat
     6421 tttgaacgcc actcatacgc atcatctgat ggcctttttt catcatgcca ttcccatgac
     6481 tacgttttcc cgtcccacac catagatcac ataaaatatt cgttccttca tgacttttca
     6541 gccaaattcc cgtacagccc agccaccaca tagcaaaagt tccaggatcc actacttctg
     6601 tttcgatttc ttcattcaag tatgtgcccc actcaggaaa tgtttctact aaccactttt
     6661 cttttgttac ttcattgaca gttttactca tttgcttgct ccccccatat cgttgttcca
     6721 acttacaagt aaatattaat gtgtatcagc atccatgaaa acccttttat atgaccgttt
     6781 gttttttata tgattttctt tcagtaatca atgattgttt cagtataata aagacagata
     6841 tgaatgttta aatgaacaac tagtcaaata aatccatgga tcaagatgtc cctcaggagg
     6901 caaaagtcat gaaaaactca cttgtcaata ttgaaaaacg tcaaaaagaa attttggaac
     6961 tgcttcagca gactgaacaa ctatcaactt tcgaattagc tgaatccctt gatgtatcta
     7021 ttagtacgat acgaagagat ttaaatttat tagaagaaaa aaacgagatc atacgcaatt
     7081 acggttattg ttcttataat tacaaaaacc agactgattt tgatcaatct ggtcctgaaa
     7141 ggattaaaca agcgattgcc agagaagcta gtaaatacat taacgattat gatacgctgt
     7201 ttatcaattc tagctcgact gcgttgaaaa cactggattt tcttcaatct aaccatttga
     7261 cagtcattac aaacaacgtg aaaatcaatt actctccgca caaagataat tgtagttata
     7321 ttttaactgg tggagagatg cgctttccaa aagaagcact agtgggcgac atcgcattaa
     7381 atacgatttc ttcagtcaat gcagacattt gtatcattgg ctgtagcgga gtcgatgtag
     7441 tacatggggt cacaacaaag atattaaatg aatcaacgat caacgaaatg atggtcaaca
     7501 agacaatcaa atgtaagatt ttagtcgctg atcatcgaaa aatcggactg acatcaaagt
     7561 tcaagattgc tgatatttca gtctttgatt atttgatcac tgataaattt tgttctgaaa
     7621 aagtattaga ggagatcaga aatacgggga taaaaaccat tcaagtagat tcattttaag
     7681 aaccaggacg taaatatgta aataggatta tcaaaaaaat gaataacaaa tttatcgata
     7741 aatgaaaaac acgctacgct caaattgata aaggaatccc aagatgtatc catccaatcg
     7801 ataccatcat aatctattgc ttcttttata accgttatat gttgcctaaa taggaatcaa
     7861 acaatatcga aaaaaggagg tatgacaaaa gtcacacctc cttttttcga ataaacggta
     7921 ttcaaatcag aacactgatt tcacaaccac tttatttcag gtcgttttca atcaccttta
     7981 ccgcgtaatc atttccgttg aaacgcgcta ttttttttgc gtccatatat ctttctaaat
     8041 aatcatcttc tcctaattgc ttatggcaag aagcaatttc ataaagcaag tattccaatg
     8101 cataagtcga tttatgtttc ttattatact caataccctt ctcacataat tggattgctt
     8161 tatcataatc ctcgatttta cggtaatagg tggcgatatc ataatataca atgcagacca
     8221 tcagattatc aaaatcagtc tcactcaata ttttaagtgt acgctccaca taaactttcg
     8281 caaaatctaa ttctcctttt gacaagtaga tcattgccac gtttgcagct gcttgtaagt
     8341 gatggatatc tgtttcggtc gtttgttcca acacttgatt aaaataaaat aatgctgcat
     8401 ctggtttttt cgcataaaga tgatcaagca gtccttgata aaataaaatt tgatttaaaa
     8461 gatcagaatt acttactttt tcctgatcga tcgtttgtaa aattttataa gattcttggt
     8521 attttgatac agaattcaat gattgaatct catttatttt tttcctgatt ttgtcttctt
     8581 ctgtttctac taatatatcg gagatctcga cattcagctt tttgcaaagc gcacttaaga
     8641 tattgatgcc tggcgacata ttcttgtttt cgatattgct gacagttgct tgttcacaaa
     8701 tgccttctgc taattgtact tgtgttaacc cctttttctt tcggtattct tttagtaatg
     8761 ctccatctac tttaatcaaa acaatttcct tccctttaaa aaattatttc tcctttatat
     8821 catacacaaa aaaaagttct aaacaaccaa aaatgtttag aacagaataa aaattttaat
     8881 tttaaaataa tattcaaatt aaacaactgg cgtatctgct ccttgatttc tttttgtgtt
     8941 atctgaataa ttatcg
//
LOCUS       DAYYDS010000099         8788 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000099 DAYYDS010000000
VERSION     DAYYDS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.102"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(266..349)
                     /locus_tag="ACHA02_002416"
     tRNA            complement(266..349)
                     /locus_tag="ACHA02_002416"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(313..315),aa:Leu,seq:cag)
     gene            877..1377
                     /locus_tag="ACHA02_002417"
     CDS             877..1377
                     /locus_tag="ACHA02_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8670716.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1567..1911)
                     /locus_tag="ACHA02_002418"
     CDS             complement(1567..1911)
                     /locus_tag="ACHA02_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8670717.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2065..2985)
                     /locus_tag="ACHA02_002419"
     CDS             complement(2065..2985)
                     /locus_tag="ACHA02_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8670718.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3172..3663
                     /locus_tag="ACHA02_002420"
     CDS             3172..3663
                     /locus_tag="ACHA02_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8670719.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3687..6062)
                     /locus_tag="ACHA02_002421"
     CDS             complement(3687..6062)
                     /locus_tag="ACHA02_002421"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8670720.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6388..7014
                     /locus_tag="ACHA02_002422"
     CDS             6388..7014
                     /locus_tag="ACHA02_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8670721.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7037..7741
                     /locus_tag="ACHA02_002423"
     CDS             7037..7741
                     /locus_tag="ACHA02_002423"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8670722.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7933..8766
                     /locus_tag="ACHA02_002424"
     CDS             7933..8766
                     /locus_tag="ACHA02_002424"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8670723.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2659 a         1676 c         1551 g         2902 t
ORIGIN      
        1 ttctttcgca tgacctcttt ttgagtttaa gtttagtctt tattccttaa ctatcgtttg
       61 ccagtatttc ttgtatcaga atctataaat ctactatttg aatcaatcta ttaggtttta
      121 aactgtccca ccttcacaca aatttttttt gatttatttg aatctggttc cgttgtttaa
      181 aatttttcct atattctttt agataatatg ttattttctt tctcatcgct ccaggtgtac
      241 cttctcttta taaaaaaaca aattatgcgg ctaagaagac tcgaacttcc acgaggtcac
      301 cctcactagc gcctgaagct agcgcgtctg ccaattccgc catagccgca tcacatcttt
      361 tagtttagga ttacatgtgt tttttgtcaa gaacatattt ttgttctttt tcatttctgt
      421 acgccctccc ttatgccatc tacacaaata attcatccga gcaactccat catctattga
      481 gggttggtga aaaaatctgt tttatccata tgatcgattt caatgttaaa cgaacgactc
      541 tgggattagt aactatttta tttttttctt catttctttt ttcctctttt tctcgattca
      601 gctatcttct ttcatttgga tattgattat ttcttctgtt gattactatc cttctggaat
      661 tctagacgct gaagtaattc acgttttctt tcttctagag atcataagta acgaatgaac
      721 gaacttcaaa taattttttt attgtaattc atatttgtat tgttcgatga ttcttaatac
      781 gatatgacac aataaataaa aataggcatg gctatttaca acaatagtgc tatagtagaa
      841 aacaaatgaa taaggttact taaagaggtg aatacgatga aactgatcaa actatgggaa
      901 gcagatttaa agaaagctta tcagctacaa actagctttg gaccaaatga gagtggattt
      961 atcaacaatg cttatggcta tacatttgct gaatttttag attatgtaaa aaaatgcgaa
     1021 aaaacgtctt taggaatcga tcttcctgag agttacgttc ccgcaaccgt ctttatttta
     1081 gaaagtgatg aacatgagta tgtaggaatt tttaatttgc gccataaact gaacgaaaca
     1141 ttaagaaacg gcgctggcca tattggttac ggcatctccc ctaattatcg taaaaaagga
     1201 tatgccacac aaggattagc attagtacta caagaggcaa aaaaaatcgg cattgatgag
     1261 gcacttttgt ctgtaaataa aaaaaatctt ggcagtctta aagcacaaca aaaaaatggg
     1321 gctgcgattt atcgtgaaga tgagaaagaa tactatacga aaatcaaact gatgtaaaaa
     1381 agaaggattc caaaaggaaa gggatttgaa tgtcctttat caatagaaca catttattta
     1441 attaacttat aatatagcag acaaaatttt ataaacaaag aaacctttta tctcttttct
     1501 tctagaaagg ataaaaggct tttttgtttt tcctatttgc ttctcatagc tagtcagcca
     1561 agatttctat cgtatcccca acgcaatccg tgcataacgg ctcatcttat ctgtcgtcca
     1621 tgctggatac cagactagtt tgacttctgc tttcgttact tctggcactt cttttaatgc
     1681 gtcatggata gattctgtta aaatatctgc taatggacag cccatagttg ttagagtcat
     1741 cttaatgact gtttctccat tttcaggatt gaattctata tcataaatca agcctaaatt
     1801 gacgatgtcg attcctaatt caggatcaat caccatttcc aaggcagcaa gaatacgttc
     1861 tttgattagt tctatttctt gtccagaccg ttcttggttt gcttcgctca tcgtacgaca
     1921 ctccttaaca cgatttttat ttatcatact ctttttttag ctgcttgtaa atgagaattt
     1981 agaagtaaga ttctcgttta aatttataaa aatgttctta ttagatgtaa tagattaaat
     2041 ttattctaaa ataaacctga ttcctcattt tttttgtgaa cgatttttta tttctggcaa
     2101 tacgccagtt gttcgataca ttttttgttc gttttcattt gcaaaccggc caaatactga
     2161 atgagttggt aaaatattga taaatgcatc gtcgtcgatt tcatgaacga tctgatcaag
     2221 atcatacatt tcgtagcggg tgatgaccat catgatcatg ttcccttgtt tttttgaata
     2281 gccaccgaca gacggcagta atgtcattcc acgtaccatc cgttttgcta gttcttctgt
     2341 tacctcttct ggcttgacgg taacgatcat tgctgttact ttttgatgac tcgtgtggat
     2401 ggcatcaatt acttgagtca ttgcataaat ggaaataatc gtatacaagg cactttccca
     2461 gttaaagaag aagcctgcga tgactacaat gataccattt agaatcagca tgtagtttcc
     2521 gactgttttt ccagttgttt gtgaaagtac aagagagatg atatccatcc caccagttgt
     2581 aaagcccatt tttaaagata atcctacgcc aagacctaat aaaacaccgc ctactagggc
     2641 attcatcaaa atattatcag tgatctgtcc gactggaagc agcatcgtca tcaccgatac
     2701 acatacaaca ttcacaaaac taaggatcgt tgctcgtttt cctactttga gaaaccctaa
     2761 tacaaacaca gggatattca aaagaaaaat aaatgttcct gtgtctatct tgatatgaaa
     2821 ccattcatat aaaagcgagg aaatgatttg agcgatccca ttcatccctg cagaaaagac
     2881 attggctgga atcaaaaaat aatttaaagc gaccgcagcg gtcaatccgg ttatcaaaat
     2941 aaccactatt ttctttaatc tttcattttt atgatacgca gtcataattt tactcacaag
     3001 cacctctccc ctactttggt attccaaatg taagaataac agtttatcta ttaaaataaa
     3061 atacttcgaa ctcaaatgag aagaatttta gcaaacaggt aagaatctgc cctaatccta
     3121 tgataagata gatgtatggt aaatatttat atgtatatca ggagggttcc tatggcaaaa
     3181 gatgctagct tcgacattgt atcagaagtc aatacagaag aagtaaaaaa tgcgattcag
     3241 caaacaacaa aagaattgtc caatcgcttt gacttcaagg attctacagt agaaatcaaa
     3301 ttagagaaca gcaatctagt tgtattgagc gatgatgatt tcaaaatcga acagatcaaa
     3361 gatgtattat ttggtaagct gatcaaaaga aatgtgccaa tcaaaaatat ccatttttcc
     3421 gaatcagaac acgctctagg tggcaaagct cgacaaactg ccgatttggt caacggaatc
     3481 gatcgtgaaa acgcgaagaa aatcactaca gctatcaaaa atgaaaaact gaaagtaaaa
     3541 gcacaaatcc aagaagatca gattagagtg acaggtaaga accgtgatga tctacaagct
     3601 gtgattgctt tattacgtaa acttgactta ccaatcgagt tgcaattcac taactatcgt
     3661 taatcaaaaa acgaggctga cgtctgtcat tcctcgtttt tttgatttac tgcttctttt
     3721 gataaccaaa aacgctgtat tccttcttcc aattccccaa cacttcgtac ttctcttgct
     3781 gtttgccaat gttgtgggaa taaacgtcga taagaaaaac tagaaaatcg ttgatctgcc
     3841 aatacaacga ttccttgatc cgccatgcca cgaatcactc gaccaccagc ttgcaagact
     3901 ttgttcattc caggaagctg ataagcgtaa gcaaatccaa ggtgttcctt ctcatcataa
     3961 tatgatttga tcagttcctg ctcatgattc atctgcggca aacctacacc tacgatcata
     4021 ctgccgatca atcggtcccc tctcaaatcg attccttctg aaaaaatccc gccaagtacg
     4081 caaaatccaa tcaatgtttc agcgggattt gtttgaaatt ttgttaaaaa tcgttcgcgt
     4141 tcttcctcat tcatgtccgt ttcttggatt agcacattgc tgtcgggata ttgctgtctg
     4201 aatgcttcca ccgcaagatc taaatattga taagaaggaa agaaaacgaa ataattcccg
     4261 acctttccat cagcaaaagc atgaatcgtc tctactaatt ttgccagact gtgttccctt
     4321 tttcgataag tcgtctctag gtaatttgcg atgaaaactt gctggtttcc ttctgggaaa
     4381 ggactcggca gtttgtaagc cagacttttt tgtcccccca gtgtttcctg ataataagag
     4441 agtggtgaaa aactagcaga aaaaagaata ctgctttttc ctttatccaa cgactgctca
     4501 agaaacaagc ttggatcaat acaaaactct tttactatca gatcgcgata cgttttttcg
     4561 actgttgttt cgtagtgatc atcataaaat tcactgacct tcaaaaaatg aagcaaatca
     4621 aaataataag gaagcaattg ttcttgtgat gggtgctcag gaaattctgc cagccattct
     4681 ttaatttttt cgcttaattg ataacctgcc tttactaaag attcagccgg tgctttttga
     4741 tgatgataat cccaatgatc ttcttttgct atttctcgga tcgaatcaaa ttcttttaat
     4801 agtttgttga aacggcgatg aagttttttg aattcctttg gtatagcttc cttacttcgt
     4861 ttcgtttttt cataagatag ttctgccgaa tacatctctc gggaacgatt caccaaatta
     4921 tgtgcttcgt caatcaaaaa cagatagtcc tcgttttttt catcttcaaa aaaacgtctt
     4981 aaataaacag ttggatcaaa taaataattg taatccccaa tgattacgtc acaccagata
     5041 ctgacatcta atgatagttc gaaaggacaa agtgtatgct ttcttgcata agtttcgatg
     5101 acttcacgag tgatctgatt ctcatgattc aaaagatccc ataaaccttc attaatccga
     5161 ttgtaataac cattggcata tgggcactga tctggattac aggtcgtctc atctaaaaag
     5221 cagatcttgt ctttcgccgt caaagtgaca ctttttactt cagctcctgt atcttttaat
     5281 gctgtcatag catcttcagc tacttgtcta gttattgttt tagccgttag ataaaagatc
     5341 ttttctccct cttcttcccc tacagctttc aaagcgggga ataacgtaga gattgttttt
     5401 cctgttccgg taggtgcttc tacaaacaat ttctgtttcg tacggatcgt tttataagca
     5461 gctaccgcta gttcacgctg acctttccta tactcatcat gggggaattt caaagccatc
     5521 aaagaggcat tgcgtacagt gcgccagttt tcttgaaaaa caagccattc tttataatct
     5581 tcagtcagct ttttaaaaaa agtttctaac tgctctcttg tctgatgttc aacctttctc
     5641 gtgatcactt cttctgccgt ctggaagtaa gtcaattgca gatttatttc ttgtaaatca
     5701 tgttcttgac tatagatata ggcatacacc cttgcttgat gaaagaaaag ttccacttgt
     5761 tctggttcta aatcttcaaa tcgtggttcg gaggttttga tttcatctat ataataaata
     5821 ccgtctcttt tgaaaatacc gtctgcacgg ccttcaacga tcaggtaata ttcatctaat
     5881 tgaactgttg tttttaaaaa gacttctttt tcatagtttt ctcccgcatc tttttggagc
     5941 ttgcggtgga ttctcgctcc ttctaatgca gtatggttac tttttttacg attgtcgata
     6001 cttccacgtc gcaaaataaa ttctactaag tgccgcacag atatttttga ctgtttgatc
     6061 atttccacca cctctttctc tctaagatta cctgaaaaaa cctagtttag caaaaagagc
     6121 tctctttcca aacataaaaa aacaccctgc tgattaggca gggatatagg atatagaatt
     6181 tggatttttg gatgaatcta atgtaacaag gacatattaa aatgctatga aaacttactt
     6241 tccttctctt tagaatatcc aaatactttt catatagtct gataattcag ataagtaaag
     6301 agaaataagt acctgttttt gtttcctagc aatgaatgcg ttatactaat ggatgaaaga
     6361 agttttggct atcaaggagg aatgcacatg catatcgaaa gattaaaaac aggaatgatt
     6421 gaagaaaact gttatttggt ctacaacgaa gaagcacttt tgatcattga ccctggagaa
     6481 gatgcggaac gaatcaaaca agaaatcaaa aaaacaaacc aacagccggt agcgattttg
     6541 cttacccata ctcactacga tcatatcggt gcagtggaac ctttacgcgc gtattatcaa
     6601 atcccagttt atgtcagccc actagagcaa aaatggctag gtgatccgat tttgaatctt
     6661 tctggactcg gtagacatga tgatattgct gatgttattg tctcgcctgc agaatatgaa
     6721 tttgaaatga aaacttatac tttaggaaac atgtcttttc gcgtcgtccc taccccaggg
     6781 cattctatag gaagcgtcag ctttattttt gatgattttg tcgtttctgg cgacgccctg
     6841 tttagaggaa gtatcggtag aaccgatctg tatacaggga atttagaaca attacttcat
     6901 agtattcgga cccagctgtt tgtattacct gatgaatttg cagtctatcc aggacatggc
     6961 gatgcaacga cgattgaaca ggaaaaaagg acaaatcctt ttttcaatcg ttaaaacgac
     7021 ttaggaggag aataaaatga cagaaactac actttatatc gtacgacacg gaaaaacgat
     7081 gttcaataca attgaacgag tacaaggctg gtgtgataca cccctcacaa agcaaggaca
     7141 agaaggcatt cattatttag gtcgcgggtt aaaaaatgtc gacttttcat ttgcttattc
     7201 tagtgacagt ggtcgtgcaa tcgaaactgc ccgtattatt ctaggagaac attctaaggg
     7261 aaaagaaatt ccttatttta ttgaaaaaag gattagagaa tggtgtttcg ggtctcttga
     7321 aggtggatat gacatggaaa tgtggggtgt gatccctcgt gtattgaatt ttaaaacata
     7381 tgatgaaatg tttaccacag aagtttcttt tgaacaaatt gctaatgctg tatacaactt
     7441 agataccgct gggtgggcgg aaacttatga aacattgcga gaccgtgtat ggacaggatt
     7501 tgaagatatc gcccatcact gcgaaaaaaa tggtggtgga aatgttttag tcgtatctca
     7561 tggtttgacg atcgcctttt tgcttaatct gatcgatcct aaacagcctg ttcgtgctgg
     7621 gcttttaaat ggcagcgtaa caaaggtcac ttatgcaaac ggtcgatttt ctatccacgg
     7681 catcaatgat acgtcttata tcgataacgg gaaaacacat agtcgcgact cgtttctata
     7741 atggggatat tttaattttt accagagaat cataaaaggc aaagtctgaa cgagggcttt
     7801 gtcttctttt ttctcttcgt gtacttatct gctttatttc tcaagttttt cttaatatta
     7861 aaaaacagat tgggtttctc aaaaaataac ggtatactat aagatgtatc ttaattggga
     7921 aggaaggaat ttatgttttt tgcaaatatt atcaaagcag tgattctcgg tattattgaa
     7981 ggaataacgg aatggctgcc catcagtagt actggtcatt tgatcctagc agatgaattc
     8041 atcaaactgg atgccagttc acaatttatg tcgatgttca atgtcgttat ccaattaggg
     8101 gcgatcttgg ctgtcgtcgt tctttatttt cacaagttaa acccattttc tccaagtaaa
     8161 tcaacggtag aaaaaaaaga tacgtggaca ctttggttta aagtgatcgt tgcttgcata
     8221 ccagccatca tcattggttt gcctttggat gattggttag aagcaaattt ccacaagttc
     8281 ttaccagttg ccattgtttt gatcgtttac ggtattgcct ttattattgt cgaaaaacgc
     8341 aacaaaaaca aaacacctcg ttggacttct ctgaatgact ttacgtacca agcagctctt
     8401 cttgttgggt tattccaagt cttggcactt gttcccggaa catcccgttc tggcgcaacg
     8461 atcctaggat ctatcttgat tggtgcttcg cgttttgtcg ctacagagtt ttcatttttc
     8521 ttagggattc ctgtcatgtt tggcgcaagc ggcttgaaga tcgtcaaata tttagctgaa
     8581 gggaattcat tcaaaatgga agaaactgtt gttcttcttg taggaacatt cgtatctttt
     8641 gtggtttccg tcttagctat caaattctta ctaagctatt tgaagaaaaa cgactttacc
     8701 gcttttggct ggtatcgtat tatccttggt atcattttga ttggttattg gtttattgca
     8761 atgtagaaaa aaccagaaca cttctctt
//
LOCUS       DAYYDS010000100         8787 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000100 DAYYDS010000000
VERSION     DAYYDS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.103"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..600
                     /locus_tag="ACHA02_002425"
     CDS             <1..600
                     /locus_tag="ACHA02_002425"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8670724.1"
                     /translation="DQEKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNS
                     YLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRV
                     LGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKK
                     GHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
     gene            615..1367
                     /locus_tag="ACHA02_002426"
     CDS             615..1367
                     /locus_tag="ACHA02_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8670725.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2070..2285)
                     /locus_tag="ACHA02_002427"
     CDS             complement(2070..2285)
                     /locus_tag="ACHA02_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFM8670726.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2304..2540)
                     /locus_tag="ACHA02_002428"
     CDS             complement(2304..2540)
                     /locus_tag="ACHA02_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFM8670727.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2798..4951
                     /locus_tag="ACHA02_002429"
     CDS             2798..4951
                     /locus_tag="ACHA02_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFM8670728.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4920..6335
                     /locus_tag="ACHA02_002430"
     CDS             4920..6335
                     /locus_tag="ACHA02_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFM8670729.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6368..7402
                     /gene="sppA"
                     /locus_tag="ACHA02_002431"
     CDS             6368..7402
                     /gene="sppA"
                     /locus_tag="ACHA02_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8670730.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7404..7925
                     /locus_tag="ACHA02_002432"
     CDS             7404..7925
                     /locus_tag="ACHA02_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8670731.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7922..8308
                     /locus_tag="ACHA02_002433"
     CDS             7922..8308
                     /locus_tag="ACHA02_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8670732.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         3140 a         1260 c         1561 g         2826 t
ORIGIN      
        1 gatcaagaaa aatttgccag atttagacat gactataaaa atttattgct gagtttaaaa
       61 gaaatcgcta atcaaaacca tgattcgata ttggaaaaag aaataaaaga attagagatc
      121 tattctaatt cttatctaac caataagttt gaatatcgtt atttaaaaaa tataaaaaat
      181 gaatatttga aaagtttgtt gatagcgaag ctataccaag caaataacaa taagatttgt
      241 tgtaaattcg aatgtccaag tactattatt gaagttcctg ttccaatatt tgactgtatt
      301 agggtgcttg gaattattct cgataatgca attgaagcat ctatagagag tcaagataga
      361 gaactgtctc tagctattta ccgagatgat aaacagatag aaatttcgat atcgaatagt
      421 tgtagagaga tactactctc tatagatact ctaatgaaaa aaggaataac aacaaaaaag
      481 ggacatcaag ggttaggttt atctagtata gaggaaatca ataaaaagaa tcccaatatg
      541 tttgtgaact atcaaaacga tccgtatcaa tttactactc aagttatttt gttgttttga
      601 gggaaggagg atttatgagt tatccaataa tattatgtga agatcaaatt atacaattaa
      661 atcagttaga acgaattatt gataatttca ttctttttca cgataaagta tttaagatag
      721 tattgaaaac acaaagtccg ttagaagtta aaaaatacct caaacaattc cgaccaaagc
      781 aaggaatata tttcttagat attgatttaa atcatgaagt taacggtata gaattagcag
      841 aagtaatcag aaaatatgat gttcaagcaa aaatcatttt tacaactact catgatgaga
      901 tgttacccgt aacaataaaa agaagagttg aaacgttagg atttgtaaca aaagatcaaa
      961 cactagatga gtatcgaaac gagattgttg agttattgtt attagcgcaa gaaaggatag
     1021 atgcaacaaa acaggttacg aatcaagcat ttgttttttc cataggttct caaacattta
     1081 cttttgatat aaatgacatt tattttgtag aagcttcaaa attaccacat agattatcat
     1141 tgtgtactaa agatgggcag tacgaatttt atggaagaat tagtgagctt gaaaagaaat
     1201 atcctatgtt aacaagaatt agtcgagcgt gtttagtaaa tatctttaac gttaaagaaa
     1261 tagattttaa aaaacgttca ctatattttg attcagagtt ggccagaaac tttactttag
     1321 ggaaagcaca aaaaattaag gagaagttga aagaaagcac tgtttagtgg ttttttcttt
     1381 agggagattg catttttcta ataaaataga ttccttcact agtgcaaatt tttctgaatc
     1441 ctattaaagt tgtaccaatt tttatagctg ttattagtta cgtgcataac agcaatatta
     1501 gttactggag gaatatttct atggtctgga accaaaaaag ggccaaatga atagttcttt
     1561 tcttgttttt attaagtacc ataaagggga attaaaatta aatgtatggt ggaagatgcg
     1621 aaagatttgg caaattctac taatttttag aaagtgtttt tcatttttta gttaaaaaat
     1681 ttgtcctgtc aagtgcaatc ctatgattgt acttgatagg atttgagata attactttcc
     1741 tgtttttata aatttaactt gcaggaacag gctttaatgc acatccgaca agtacattac
     1801 caatacctcc tgtaacggcc gaaataggtc ctcctgcagc accaactaca gcgctgcaag
     1861 tccttttgta aaaatatctc taccattaca tttccaaaga atttttatct gactttaata
     1921 aaagataaaa atttaatata attataaaaa ctagcacaag aagctttagt aaaaagttct
     1981 gtccattcca aacttttgaa aagataaaga acatacctat gttcgtaatc gacacaaacc
     2041 aatcttgagg tttgatattt tttaattgtt tacgcaccgg tccacatgct gaatccagca
     2101 cctaataaag ctccacctac accacaagca gctattgcat aaggtcctcc ggctgaacaa
     2161 agttttactc ctgctgttgc cccagaaaaa gtagtgccta aaactcccac ggcactccca
     2221 ccttgaatat ttttcaactc ttctgaagat aatataaatg ttgtttttgt gtttttaata
     2281 tgcatactac ttattctcct tttttatttt tttcgagcat cccagccagc tgcagcacct
     2341 ccagcgacag ctccatataa ccctccaaca acaccgcaag ctggtccgca aattgctgtt
     2401 cctaaacctg caccacctac tgcccctgtg gtaagtccac ctacaacttg ccccaatttt
     2461 ccccaagcat tattgcctcc accaacagtt tgtgcatttc cacaatactt agttccttta
     2521 cattttgcat gtttaaacac gcttctttaa ataatatttt ttacggtgct actgtaacat
     2581 acgtatttga ttaacagtaa aaatcttcct gaaacttatc aagtttattc ttgaatagta
     2641 aaatttccaa aataaatttg attcaggaat gaaaaagtta gtgttcaaga actgttttca
     2701 aagtttttgt ggaattatga gacaatattt tcactgtatt aaagctatat tgagaatttt
     2761 tctataaaaa ttttttagaa ggagttgtgt tagataagtg ataaaatcat ttccttttgt
     2821 gcagcaacaa gatgaaaaag attgtggtgt tgcttgcctt tcgatgattt taaaatattt
     2881 taaaactgat atcccgattc ataaattacg ggacctatct gggactgatt tagagggtac
     2941 ttcagcattt ggattgaaaa agacatttga aaaattgtct tttgattgta tggctattca
     3001 agcagatagt gagatctgga aagaaagaga tcttatctta ccattaattg cacatatatt
     3061 ggttgataac agctatatgc actacgttgt aatttataaa acgaagggaa acgtactatg
     3121 gattgcagat cccgctaatg gaaaaaatag aaaaacgatt gaagcgtttc aaaaagaatg
     3181 gacaggtatt cttttattac ctatgcctag agaaacttat attcctatta aagaaaaagt
     3241 agctggatta agttcttttt tgcctataat ttggaaacaa aaaggattag tattccacat
     3301 cgttttagca tcgattttca ttacgttttt tggcatagga agctcatatt attttcaagg
     3361 tattcttgat tttttcatcc ctaatcaagc acgttcaact ttaaatataa tttccattgg
     3421 cttgatcgct gtctatttat ttcgggtatt gtttgaatat agtcgtagtt atcttcttat
     3481 tattttagga cagcgaatga gtatggcaat tatgcttcag tatttcaaac atgtgttaac
     3541 tttaccaatg aatttttttg ctacgagaaa atcaggggaa attatttctc gttttcttga
     3601 tgccagtaag attattgatg ctttagcaag tgctacgtta tcaatatttt tagatattgg
     3661 tatggtgttg cttgttgggg ttacgttagc tattcagaat agtacgttgt ttttaatcac
     3721 tctatgctct ttgccttttt atgtagttgc aatattcgct ttcgtgaaaa gctatgaaaa
     3781 atcgaacagg gaagaaatgg cagccggtgc agctgtaaat tctagtatca tagaaagtct
     3841 taaaggaata gaaacaatta aagcatatag cggtgaagag aaagtatata accgtataga
     3901 ccatgaattt gttaaattaa tgaaaaaatc atttcgtacg gttacgcttg ataatattca
     3961 acaaggaatt aaacatttga ttcaattaat tagcagtgca ttaattttat ggctaggttc
     4021 ttattttgtt atggataata gaattagtct aggtcaactt atcacataca atgctttact
     4081 agtatttttg acagatcctt tacaaaacat tattaattta caagtgaaaa tgcaaacggc
     4141 tcaagttgca aataatcgtt taaatgaaat tttcgcgatt gattcagaaa aatcgaacca
     4201 gtcttttgaa tatatgcttt ctgaacaatt atttcaacaa gatatcactg taaaaaacgt
     4261 ttcattctcg tatagcatga aggccccaac gttgaacgaa atttcatgta caattccttc
     4321 gcacagtaaa acggctatag ttggagttag tggttctgga aaatcaactc ttgcaaagtt
     4381 atttgttcgt ttttatgaag catcagaagg atcgatccat tatggaaaca ttaatcactt
     4441 agacattcct tttcagatat tacgcgatcg agtgacatat ttgcctcaag agtcattttt
     4501 ctttagtgga acaatcattg agaatcttat attcggttta gatagcccac caagtttcga
     4561 acaaataata gaagtttgtg agatagtcca actaaatgaa tttattaatc aacagccatt
     4621 acgttacgag acattgttgg aagaaggtgg aaataatctt tcaggagggc aacgacaacg
     4681 attagcctta gcacgggctt tattaaaaaa tccggacata ttaattttag atgaagcaac
     4741 tagtggatta gataccttgt tagaacatgc aattatgaat tatctcctca atttaaaaga
     4801 aaaaacgatt atttttattg cgcatcattt accaatagca aaagcatgtg atcaaatttt
     4861 agttctccat gaagggcatt tggtagaaaa aggaacgcac gatgaacttc gttttgccga
     4921 tggtatctat caaaaattat gggaaatcta ggagagggaa acaatttatg cagaataata
     4981 attggttcga acattctagt atctatacta aacaacatca tagcttctac cgttgggtgt
     5041 tataccctgt aattcttttt ttatgtatta ttggattgtt tttaaccttt gctaaaaaag
     5101 aggttgttat tcggacaacg gctcaattga accccgaaaa aatagagaag ttacaagtgc
     5161 cattagaagc taaaataatg gaaaacttcc taaaagaaaa tcaatttgtt cataaggacg
     5221 atgtgttggt tagattggat tgttctttaa tagagaatga aaaggctcag atagaacaag
     5281 aaaaccaacg tattactcaa caaataaaga tggctcagct atttattgaa agtataagta
     5341 aaggaaaaaa tttgttttca acggatgaca gttttggcta cagtaatcaa ttaaagagca
     5401 tgttgtcaga aaaagaatca ctccgctacg ctttgaagca aagtgaatta aatgatcaaa
     5461 agcaattaga agtatacgaa aagacaaaaa gacaactaga aaaacaaatt gagagttcag
     5521 atagtaaatt acaagaatgg caacaagtac aggtagcttg gagtaataat caatcattaa
     5581 aagatttttc aaaagaaatg atggcaaact atgagaattg gcaagaacaa ctaaataatg
     5641 tttctgatga tcaaaaaaat caagtgaaac tgacaatttc agcaagcata aatgaacaaa
     5701 ttgagcaact aaaaaaagaa gtagaacagt atcagtcaga aaaagctaaa ttagttaaac
     5761 caactacttc tgagaatgac agaattagtc aaacggaaaa aggaaagcaa gagctagaac
     5821 aagcaataac aactgtaaaa caaacagtta cagagctaaa tgaaaaacaa gagaagaacc
     5881 agtcagttat caaaagtttg gacgatcaac tttcaaaagg aatactaaaa gctcctgtat
     5941 ctggaacggt tcatttaaat gaagaaacaa agggacaagc agaactagcc aaaggaacag
     6001 tgcttgcaga aatttatccg atccacgaaa aatcaaaaat gaaatttaca gcacttttac
     6061 ctgcgaatga aagtacctat attaaagaag ggatgaaggt tcattttaaa ttggatcaaa
     6121 aaggtggggc tccgattacg atcgacggaa ctttagatga aatttcagaa aattctactg
     6181 ccactgagaa aggtgttttt tatgcagtga aaggaacgtt aaaaacaact caatcgtttc
     6241 cgtttcgtta tggattaact ggagagttgt ctttaattgt aggagaaaaa acatattggc
     6301 aacaaattgt cgatgtgttt ctaaataaaa aataattatt tttactaaga atataggagg
     6361 agtttatatg aataaaagaa gatggattgc tgtgggagta gcgtgcggat tattgttact
     6421 atccattatc gtcgcgctaa ttccaggaaa agataaagag gaagcatcaa atacaaccct
     6481 aacaggaata aataagattt tttacggatc taacgaaatc acagaagaaa cgttagaaga
     6541 aggcgcatca aataaaaaga ttgtcaaact atccgtcaat ggagttattg cagatacagg
     6601 tgaaagcaat ctttttagta gggaacaata taaccatcag aattttttaa cacagctaaa
     6661 gaaaattcaa gaagacaaag cggttaaggg agtgttatta gaagtaaatt cacctggggg
     6721 aggaatatat gaaagtgctg agattgctaa agaaatggca aaaattaaaa agctagatat
     6781 tcctatctat actgcattta aaaatacagc ggcaagtgga gggtactaca tttctgctgg
     6841 ttcagataaa atatttgcta cggaagaaac aacaactgga tcaattggtg tcattatttc
     6901 tggtttaaat tattctggct tattagaaaa acttggcgta actgatgcga cgtataaaag
     6961 tggtgcatta aaagatatga tgtcacctca acacaaacct tcagaggaag agaataaagt
     7021 tatacaggaa tttgtgatga gtgcttatga tcgttttgtc aatgttgtag caaaaggaag
     7081 aaatatggat actaatgcag tgaaagaact tgcggatgga agaatttatg atggtaacca
     7141 agcagtagaa aatgggttgg tggatcaaat tggttactct gaagatgcat tagattccct
     7201 aaaaaaagag aagaaattaa ctgatgcgac ggttatagaa tataaaaatg acacaactgg
     7261 ctttgcttct tcttggttag gtaataagat tgctgaatgg caagggctaa aggcaactac
     7321 atcagatcgt gtaatttcag tttttgaaaa acttggattt tctgatactc caaaaccaat
     7381 gtattactat ggagggctat aaaatgaata agaagagtgg cattgaaact ttgcctaatt
     7441 atgtatttgc gggattttgg ttacgatttt ttgcttttct tattgattta atttgtatta
     7501 gcatcatcac ccatgctact ataggtctct tttacaaaat catgggactg gaatctgaca
     7561 aatccattat tactttttat ggtatagcgt cattagctat ttatttaacc tattttgtac
     7621 ttttgactaa gctgaattca ggacaaacaa ttgggaaaat gatttttggg ataagagtag
     7681 tgagctttga ggaaaaagag ctaagttggc aaactatctt tattcgtgag tgtatatgta
     7741 ggtttatatt aaaaaatttt gtatttcaaa ttgggtattt ggtggctgct ttcacgccca
     7801 agaaacaaca tattggggat tatttttcta acacgtcagt ggttgttatt cgttcattag
     7861 aagctattaa ctatggtcaa aaaatttcta attctaaaac acttaatctg gagagaatta
     7921 aatgaataag agaaaaacag agtttatttt gattttaatt agtggaataa cagggatttt
     7981 atgtagtctt ttagcattga tgggtggtat gtttctatct tctattgatt caactacttt
     8041 aaatactaca gaactaacca gtaaggatat agatatgtta gtcaatctaa gcgggaatgt
     8101 tgttttattt ggaggaatta ccctagtttt ttcgataatg attttcataa taggtttttt
     8161 agtaagaaaa aatcaaaaaa cagtggtcta tggtattagt ttactcattt tatctattat
     8221 tccatttttt cttttaacat tattgtgggt aattcctgga gttttaggta ttattgcggg
     8281 attgatgctt ctaataagaa aatcttaatt aggcaatgtg tagacaacgt gaaacgatta
     8341 atcaattaaa agctaaatac gttttgagaa atgataactt gtagtcgatg gaagacatgt
     8401 agataaaaaa actacgtgtc ttttatttat tctaattttt tcgaaaaaaa gacactctct
     8461 gattgttgta ttaatggaaa aggggaaagc agcaagtaaa tttagttttt ctattaaata
     8521 ttcttcactt tttaaccttt tatagtactt agatttaatc ctatatgttt agagagttca
     8581 ttggcagcat gattttgcaa agcttctcga attattgtta tattagaaac tttactgttc
     8641 ataattatgc ttctttttta actcaaagtc caaagggcta cagtggactt tgagttaaaa
     8701 ataaaaagcc ctagttattt tgaaaaataa aaaaagaaca gcgtaaatgt tttcgaaatg
     8761 ctttcgcacc aacatttacc tgttccg
//
LOCUS       DAYYDS010000101         8681 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000101 DAYYDS010000000
VERSION     DAYYDS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.104"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1475
                     /locus_tag="ACHA02_002434"
     CDS             138..1475
                     /locus_tag="ACHA02_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8670733.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ACHA02_002435"
     CDS             1523..3652
                     /locus_tag="ACHA02_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8670734.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ACHA02_002436"
     CDS             3627..4316
                     /locus_tag="ACHA02_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670735.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ACHA02_002437"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ACHA02_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="ACHA02_002438"
     CDS             complement(5546..5740)
                     /locus_tag="ACHA02_002438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670736.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="ACHA02_002439"
     CDS             complement(5790..6230)
                     /locus_tag="ACHA02_002439"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8670737.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="ACHA02_002440"
     CDS             complement(6234..7079)
                     /locus_tag="ACHA02_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8670738.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="ACHA02_002441"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="ACHA02_002441"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8670739.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2654 a         1615 c         1854 g         2558 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat t
//
LOCUS       DAYYDS010000102         8576 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000102 DAYYDS010000000
VERSION     DAYYDS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.105"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(110..565)
                     /locus_tag="ACHA02_002442"
     CDS             complement(110..565)
                     /locus_tag="ACHA02_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670740.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="ACHA02_002443"
     CDS             complement(719..1927)
                     /locus_tag="ACHA02_002443"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8670741.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="ACHA02_002444"
     CDS             complement(2039..2371)
                     /locus_tag="ACHA02_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8670742.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="ACHA02_002445"
     CDS             2519..3394
                     /locus_tag="ACHA02_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8670743.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="ACHA02_002446"
     CDS             complement(3476..4057)
                     /locus_tag="ACHA02_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8670744.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="ACHA02_002447"
     CDS             complement(4063..5322)
                     /locus_tag="ACHA02_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8670745.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="ACHA02_002448"
     CDS             complement(5324..6370)
                     /locus_tag="ACHA02_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670746.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="ACHA02_002449"
     CDS             complement(6605..6880)
                     /locus_tag="ACHA02_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8670747.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="ACHA02_002450"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="ACHA02_002450"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8670748.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT         2414 a         1796 c         1451 g         2915 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaa
//
LOCUS       DAYYDS010000103         8494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000103 DAYYDS010000000
VERSION     DAYYDS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.106"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACHA02_002451"
     CDS             complement(<1..149)
                     /locus_tag="ACHA02_002451"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8670749.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACHA02_002452"
     CDS             complement(593..1714)
                     /locus_tag="ACHA02_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFM8670750.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACHA02_002453"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACHA02_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFM8670751.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACHA02_002454"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACHA02_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFM8670752.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACHA02_002455"
     CDS             complement(4304..5215)
                     /locus_tag="ACHA02_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8670753.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACHA02_002456"
     CDS             complement(5232..6239)
                     /locus_tag="ACHA02_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8670754.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACHA02_002457"
     CDS             complement(6236..8365)
                     /locus_tag="ACHA02_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8670755.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACHA02_002458"
     CDS             complement(8370..8480)
                     /locus_tag="ACHA02_002458"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670756.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAYYDS010000104         8407 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000104 DAYYDS010000000
VERSION     DAYYDS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.107"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHA02_002459"
     CDS             476..1339
                     /locus_tag="ACHA02_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8670757.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHA02_002460"
     CDS             complement(1468..3090)
                     /locus_tag="ACHA02_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8670758.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHA02_002461"
     CDS             3339..3860
                     /locus_tag="ACHA02_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8670759.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHA02_002462"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHA02_002462"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8670760.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHA02_002463"
     CDS             5941..6759
                     /locus_tag="ACHA02_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8670761.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHA02_002464"
     CDS             complement(6862..7059)
                     /locus_tag="ACHA02_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670762.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHA02_002465"
     CDS             7228..7806
                     /locus_tag="ACHA02_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8670763.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHA02_002466"
     CDS             7901..8038
                     /locus_tag="ACHA02_002466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670764.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2539 a         1504 c         1649 g         2715 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctc
//
LOCUS       DAYYDS010000105         8275 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000105 DAYYDS010000000
VERSION     DAYYDS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.108"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            296..829
                     /locus_tag="ACHA02_002467"
     CDS             296..829
                     /locus_tag="ACHA02_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670765.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(955..1632)
                     /gene="nth"
                     /locus_tag="ACHA02_002468"
     CDS             complement(955..1632)
                     /gene="nth"
                     /locus_tag="ACHA02_002468"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM8670766.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1639..2337)
                     /locus_tag="ACHA02_002469"
     CDS             complement(1639..2337)
                     /locus_tag="ACHA02_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8670767.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2605..3789
                     /locus_tag="ACHA02_002470"
     CDS             2605..3789
                     /locus_tag="ACHA02_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8670768.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3795..4238)
                     /locus_tag="ACHA02_002471"
     CDS             complement(3795..4238)
                     /locus_tag="ACHA02_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8670769.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4464..4751)
                     /locus_tag="ACHA02_002472"
     CDS             complement(4464..4751)
                     /locus_tag="ACHA02_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8670770.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4922..6274)
                     /locus_tag="ACHA02_002473"
     CDS             complement(4922..6274)
                     /locus_tag="ACHA02_002473"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8670771.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6547..6993)
                     /locus_tag="ACHA02_002474"
     CDS             complement(6547..6993)
                     /locus_tag="ACHA02_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8670772.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(7229..8155)
                     /gene="cysK"
                     /locus_tag="ACHA02_002475"
     CDS             complement(7229..8155)
                     /gene="cysK"
                     /locus_tag="ACHA02_002475"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8670773.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2277 a         1656 c         1320 g         3022 t
ORIGIN      
        1 aaaaaagaaa ccacgtctac aagcgtggtt tcttttttgt gtctatgtaa aaaacagcta
       61 caaataaaaa tcatgtacca agctgtatta catgattttt tataagacta tccagcatat
      121 acatgtgtga tctaacattt agcttaaaaa ttagaaaatc catttttctt ttatcattac
      181 atatacttta tccatttact tgtttataat taaatcggct aaatacacac tagtctttta
      241 tttagtgcca cttcttaata atcccattgt tattatcaaa taaaaaatac aatatatggt
      301 tttgaataat tttttgaaag aaggcttcat tttgagttat ctgatagtat gcttaattat
      361 cataatttgt ctgcaccatc tttttttatc gaaaacaata cctcgttatt ccaagaataa
      421 aaaagctgaa aaattttgtt tattattaaa taaatttact ttaattgctc ctatccttgc
      481 atttattatt ttttctgtac ttttaagcac tactcttaaa ggaaaattta tggaaagaag
      541 ctctcatgca atgattctca cttttttatg gttgttgttc accagaatct atatttttct
      601 gatgagcctg aaaccgccca aatccattag tttatgttta gttataaatg gtattttttt
      661 attatcactt atcattttta ttacaccttt agatagatac gtcacttatt tatataatcc
      721 tttggaatac tggacttatt ttataggtat attggaaggc attattttct acatcgggta
      781 ttttcccaat aaaaataaca acttttattt ttatcgcaat aaattgtaat aagttaccat
      841 cctagctact gagtatgtac atttactttt tcatgcaaaa aagataaacg aaacagcttt
      901 tctccataat aacaagataa ttcatacatt tatttgcaat taaagaaacc acgtctacag
      961 gcgtggtttc ttttttagcg ccttttcttt tagacgcatc cgatttttcc catcttgaca
     1021 tattgatagc aaaggacata cctcgcactt gggatttctt gccgtgcaat gatatctacc
     1081 aaaaaaaatc agtgtatgat gtgtctttac ccataattct tgtggtactt tccgcatcag
     1141 tgtttcttct acttccatga ccgtagcatc tagtttgcag atcctcaggc gttttgagac
     1201 acgttccacg tgggtatcta ctgcgattgc tggtatcccg aatgcatccc ccaatacgac
     1261 atttgctgtc tttctgccta caccaggaag agacatcaat tcttctcttg acgttggcac
     1321 ttgcccatcg aaacgttcaa tcagctgctg tgcacacgct ttgatgtttt ttgctttatt
     1381 tcgatacaaa ccaatcgttt ttattttaag aatgatttca tcgattgaag cttctgctaa
     1441 cgcatcgggt gtcggaaaag aggcaaacaa atctggcgtt gctttattca ctgatacatc
     1501 tgtcgcctgg gcacttaaga tcactgcaat caataactca aatggattat tgtgcttgag
     1561 ttctccatgt gcttcgggaa acattccata catcgtttct agagcttcca ttgttttttg
     1621 tttgcttaac atattccgct actcctccaa ccaatttttc agtgagactt tcggcaattc
     1681 ttcttgttga gctgactgtt ctacttctct ttgaagaagc tgctgttttc ttcgtttttg
     1741 gtcttcttcc acttgttgtt ttgtacgtag attttttctt tcccacgata gcaaaatgcg
     1801 gtcaacgtaa ttaaaactat aagcctgatt taatacggct tcccgcagtg ctagcttcaa
     1861 aatttccggc tgataatggt cttcttctag ccattgtccg atccgctgga actcgatcga
     1921 agaaagcggg cgtccaaatt cttgttcaaa taattgatac actgactgga tttctttttc
     1981 ttgtgttttt tcttcgtatt ttttttcttg tgtttttaaa tactcaccta atagatcata
     2041 gatggggtaa agattatatc gatctgcttt tttaccgttg acaatcgtcg tttctatctt
     2101 gataaatcca tttgttacga gacgatccag tatttgaaaa atctgatctt gtttgattcc
     2161 catatccata gcaatcatct gcaaatcagg gaaagtatct ccttcaagct gtgccatatg
     2221 caactgtaga acaaataaaa attcttcaga ttgtagaccg atcctatgat aatttttaag
     2281 taaaagatta gatatgactg tttgtccagc gcttaagtaa ttttctaaat tcaacatgat
     2341 ttctcctccg ctcaatcaca gtatacgtaa aaaaaacatg ggaagcaaga acaggtttct
     2401 tttctattta cttcactgat cggtcagatt tacaatgtcg tctacttgat ttttgtttgt
     2461 tcatgttttt gatgcgtatc ttcacttttc ggaataaata aattaagtag atttcgctgt
     2521 tccttagaat gttgacaagg aaacaaaatt actatacact atttttgttt ccaagttaac
     2581 attattgcat aaaggagttt ttacatgtac agtttcacaa ataaacattc taaagttttt
     2641 ggatacgtct ctgtcttatt cactggatta ggatttacta tcgtcagtcc tgtacttccg
     2701 tttttaacgc tgccttattc tcactccata catcagcaag ctttctatat cactttgctc
     2761 atgtctgttt atgctctagc tgccttctta tctgccccca ttctcggtag cttaagtgat
     2821 cattttgggc gtcatccgat ccttattatc agtttattag gatcaagtat cggatatttg
     2881 ctttttggtt taggaaattc gatatggatg ttgttccttg gtcgtatcat tgaaggattg
     2941 acggcaggag aaatctccac cttatatgct tactttgccg acatcacgga acctaatgaa
     3001 agaaccaaag tttttggatg gatgggtgct ttagtcggta taggaacaac gctgggtcct
     3061 attatagggg gtctattagc tgagttgggg aattctgttc ctatatttat tggagcactc
     3121 tttactttct taaatgccgt ttatggctac acatttatgc tagaaagcct tccaataaaa
     3181 aaacgttcag tagatttaag tttctcccat gtaagacctt ttcaccaatt acaacaatta
     3241 ttcaagattt catccgttat tcctttattg acagcaggtt ttgctgtttg gttggcagct
     3301 ggctctcttc aatctatctt ttctcaattt tcgattgacg cctttcagtg gaaggctggg
     3361 ttggttggtt taagcttctc tttgattgga atcttggata ctgtctcgca attgttcatt
     3421 atgcctcgat tgctcaaaaa attttcagaa cagcagatca caagaatagg gatgttcagt
     3481 gaaatcctcg cctatctatt catcacatta tctggaattc ttctactgcc gattttgttt
     3541 ttatttggga ttatctgtta tggtttcggt gattctattt ttactcctgt attcaatggc
     3601 caactatcca attctgtctc agataaccag caaggtcttg taatgggcgg tacccaaagt
     3661 atacaatctc tatcgcgcgt catcggcccg ctaatcgcag gacagcttta tgctgcagca
     3721 ccatttcttc cgacggtatt cggatttttg cttatgattt ttgcctatta tcactacaat
     3781 aaaaaatagt agcttcattt gctacttcgt ataaattctc tgtatcgacc gataaaacga
     3841 aatactgtgt caaattcttc ttttgacata ttttcaaata cagtagcatc tcgctgtacc
     3901 catttttgat ggagttgctc gtgtctattc cttatttttt gtccgattgg cgtcaattga
     3961 aaatagattt cttttttatt ctcttcgctt tgataagtgt ttatgatccc tctagctatc
     4021 agtttttttg ttagcttact gatggctcct ctagtcatgt aactagcatt cgctaatttt
     4081 gtcacgttag gatcagggat tttcccgata tgttcaatca cttctagttc atttagagta
     4141 aatcctgcaa attctttttc catttctggt ttattacgat gaataatctc attagctaaa
     4201 tccagatatt cttctattag cagttgtctt ttatccattt ctacaccttc tttttctttc
     4261 attatattgt ttttttgttt cctattcaac aaagataaaa atattttgta ggaaaaccca
     4321 atctgaaacc aagaaaaaaa ggggttgaca aagcttgtca taaccccttt ttctgattaa
     4381 aagatagtaa agagacagtc atcggtaatt gtgatgattt tttgtcatca cttaaggatg
     4441 agcggccttt tcaaaaactg tctttaaaga tcatcatcag tttctagcac atatggctga
     4501 ccttcatatg tgtactctgt attcaatgtg ccgtcatttt catctgagta gatgcgtcta
     4561 ctgaaaggaa aacgatgaga aacattatct ttggcgtcat ttgctggcat cgtgtaatat
     4621 tcctcttgcg tttcattcaa ctgagagagt acttgatctg ccaactcatc aatattgatc
     4681 gttaatttct tcgaagcact tttataatct tctaaaaatg catcttgttg gacaaaatat
     4741 tctttattca tcttctttct cacctttctt ttcaaaatca aataaagctg cttgttcttt
     4801 tatgtaacag ctttatcttt gctgcttatt gtgacaaaaa attcgaagaa agacaactaa
     4861 ttgtactttt cacaaaaaaa gacagagggt cgtgacaaaa ttgtgtcaca accctttttc
     4921 tttattgtgc agtttcgatt tcgcctagcg ctgcttgtgc aagtaggttc aagtaattcc
     4981 atgggcggtc aaaatgtggt tggaagaaga aatctgaaag tgcgagatct tctacagtca
     5041 ttttattttg aacagctaaa gacatggtat ttgctgattg tgtaatgtcg tatttggaca
     5101 tgaactgagc acctacgata cgattcgttc ctttttcata gactagctcc atcattactt
     5161 tttcagtagt tggcatgaac tctggacgat aattatcttc aaatacagtc gcttggactt
     5221 ctagatcgtt caaagttgcg ctttctttgg tcacacctgt cgaaccgatc gtccagccaa
     5281 ataggtaaag tcctgaagtt ccttgagtac cacgatacgc cattttatga tctgtcaagt
     5341 tatttcccac aagcaaacct tgacgaacgg catttgttgc caaaggaata tagttttgcg
     5401 tgccacttgg attgtagttg actactgcag agtctccagc tgcaaaaatg tctggattac
     5461 ttgtctgcat gtattcattc actttgattg ccccgtttgg cagtgtatct acttttcctt
     5521 cgatcagttt tgtatttgga cggaagccca cacataaaat gaccatatct gtatcaaatg
     5581 tatctgttgg tgtagctact tgtttcactt ttccttcttc atctgtgata aattcagtaa
     5641 cattttcgcc tagcgcaaga gtaacaccgc ggtctgtcaa ttctttttct aagatatctg
     5701 tgaatggttt atctagatat ttattcaaga tgcgatctaa tccatcaatc aatgtcactt
     5761 gttttccaga ttcgacaaaa gcttctacta attcaatacc gatatatcca ccaccaacaa
     5821 ttactacacg ttttgcttct ttcgcttgtg caataatttc atttgcttga ttgtagtttt
     5881 tgcataggag tacatttttc gcatcgattc cactaatcgg tgggataata ggccatgaac
     5941 cagttgtcat cactaattta tcataagaaa ccgtttctgt ttctcctgtt ttcagatctt
     6001 ttgccgttac tgttttattt tctgtatcga cgtctgttac atcgtgttcc attttaactg
     6061 ttgctcctaa tgaagataat tcttcaggat ttgagtagaa caagcctgct gggtccttta
     6121 ctactccgcc gacataaagt gcgataccgc aagataagaa agaaatattg tcatttcttt
     6181 catatactgt cacttctgct tctggatgat tttttaaaat actttttaca gctgcagtac
     6241 ctgcatgagt acagccaatt actactactt tcatttatgg tccctccgat taacattttg
     6301 tccttcgaca aaactactat aacagaaata aaacggatac aacagtttaa tgcttgtgaa
     6361 ttcacaaagg tttgtttgta gtttcattcc ttttcaagca taagaaccca atatctacaa
     6421 gattcttatt ttaataatat tcacttttat atctaagatt gtgaataaaa gacaaaaaaa
     6481 taaaaaagct agcgtttttt ttacacagct agcttttttt aggtataaca cttgtagaaa
     6541 ctgtttttac atttgcgcct tttttgctga ctctttacaa tccggacaga gtccatacaa
     6601 ttccaaccga tgttcattaa tctcaaaatc agtcagtctg cttgcagcca tttctatatc
     6661 ttcaagccct gggtaatgaa aatcaacaat cttaccgcag ttttcacaaa ttacatgata
     6721 atgacgcttt gaactgaaat caaatcggct agaagcatcg ccgtacttca tttcttgaac
     6781 aaatccaatc gaagtaaata agcgaaggtt attgtaaaca gtcgcaacac tcatatttgg
     6841 aaaccgatgt tcaagtgctt tataaatctc atcagccgtt ggatgggaat gatgttcaat
     6901 caaatattct aaaaccgcgt accgttgagg tgtgattcga atgttctctt tttttaactt
     6961 ttcgataacc tcttcgacta acttattttc catgtaaaca ccccacgttg ttattctcgt
     7021 cttatcatac tatttttaca ttttaaaatc cacttaacat gaaaagaaaa ttcaatttta
     7081 ttgtatttta atgaaaaaag acgtaaaaca gtcgcgttca ctattctacg tctctattca
     7141 aataattttt ctcatcataa atttttgtgt gttcttttca tctttaaaag ttttttacat
     7201 aaacctatct ggagaaaaac caagtgcctt atatctcttg gtaaagggct gtagataaat
     7261 aacgttcacc attgtcggga accacagcta ataccttctt gccttttcct aattcttttg
     7321 cgacttttag cgcggcagca attgcagcgc cagatgagat gcctactaaa atcccttctt
     7381 ttcgcccaac ttctctagct gtttccatcg cttcgtctcc actaatagaa aggactttat
     7441 catacacaga agtatccagt gttttaggaa caaaacctgt accgattcct tggattttgt
     7501 gaggtcccgc ttctttccct tctaaaatag cagattctgc tggttctacc ccgattaatt
     7561 cgattttcgg atacacacgt tttaattctc ggccagcgcc agtgatcgtt cctcctgtac
     7621 cgatacctgc aacaaacgcg tccaagccat ttactccaaa tgcttgatgt atctcaggac
     7681 cagtcgtctt ctcatgcaca agaggatttg cttcattttc aaattgtaat ggtaaaaagt
     7741 agccgttttc ttttgccaaa cgttctgctt ctctgattga tccagaaata ccatccgcac
     7801 caggagtcaa aatcaactct gcgccgtacc ctttcatcaa taaacgtctt tcgatactca
     7861 tcgtctcagg catcacgatg attactttat atccttttgc aactcctacc atcgacaatc
     7921 cgattcctgt atttccagaa gtaggttcaa tgatagtatc acctggtttc agtagaccat
     7981 cgtgttctgc tttttctatc atacttaaag caatacgatc ttttacactt cctcctggat
     8041 taaaaaactc aagctttaca aaaacatctg ctgaatcttc tggtacgatt ttatttaatt
     8101 tgacgattgg cgttttgcca atcaattcgg tgactgagtt atagatagct gtcatcttca
     8161 attcctcctt gtctttgctc ttcttactta tcataataca atctgttcat ttatggcgag
     8221 tgattcgtct ttgactagca aaaaaaagac aaagatatac catatatggt aattt
//
LOCUS       DAYYDS010000106         8171 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000106 DAYYDS010000000
VERSION     DAYYDS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.109"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1248
                     /locus_tag="ACHA02_002476"
     CDS             94..1248
                     /locus_tag="ACHA02_002476"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8670774.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="ACHA02_002477"
     CDS             complement(1270..2022)
                     /locus_tag="ACHA02_002477"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8670775.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="ACHA02_002478"
     CDS             complement(2386..5052)
                     /locus_tag="ACHA02_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8670776.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="ACHA02_002479"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="ACHA02_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8670777.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="ACHA02_002480"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="ACHA02_002480"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8670778.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2372 a         1697 c         1457 g         2645 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat
     8161 tcgctattct a
//
LOCUS       DAYYDS010000107         8155 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000107 DAYYDS010000000
VERSION     DAYYDS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.110"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            384..572
                     /locus_tag="ACHA02_002481"
     CDS             384..572
                     /locus_tag="ACHA02_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8670779.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            650..1663
                     /locus_tag="ACHA02_002482"
     CDS             650..1663
                     /locus_tag="ACHA02_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8670780.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1730..2764)
                     /locus_tag="ACHA02_002483"
     CDS             complement(1730..2764)
                     /locus_tag="ACHA02_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8670781.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2879..5104
                     /locus_tag="ACHA02_002484"
     CDS             2879..5104
                     /locus_tag="ACHA02_002484"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8670782.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5122..7005
                     /locus_tag="ACHA02_002485"
     CDS             5122..7005
                     /locus_tag="ACHA02_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8670783.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6986..7960
                     /locus_tag="ACHA02_002486"
     CDS             6986..7960
                     /locus_tag="ACHA02_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8670784.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2645 a         1479 c         1714 g         2317 t
ORIGIN      
        1 tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg tgcaaaatca
       61 aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa
      121 ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca
      181 tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga cgacgtagca
      241 gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg
      301 tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt
      361 tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg
      421 aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt
      481 caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag
      541 cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata
      601 acactatggt ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa
      661 aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt
      721 tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa
      781 tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa
      841 aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa
      901 aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg
      961 agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt
     1021 ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt
     1081 gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga
     1141 acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat
     1201 acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag
     1261 tctgaaagga accatttctt attggggacc tgttgatttg aagctaacag cacagcagca
     1321 ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac
     1381 aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa
     1441 ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca
     1501 agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga
     1561 tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa
     1621 ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc
     1681 gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt
     1741 ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg
     1801 gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg
     1861 atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat
     1921 tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc
     1981 gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt
     2041 cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat
     2101 gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc
     2161 tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc
     2221 cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca
     2281 tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga
     2341 cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa
     2401 caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat
     2461 tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca
     2521 aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa
     2581 tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag
     2641 ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca
     2701 acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa
     2761 ccatttttac acactcctca aaaaaataaa aaatttttat tgacaaaatt gtttagtaaa
     2821 cagtatacag gaattataaa aaaaagtaaa cggtttccgt tttgttggga gtgaaatgat
     2881 ggatattacg catagaaaaa cagataaaaa tttcatctta tttgatgaaa agagccgagt
     2941 cttccattta cgaaatacat ttctttctta tctgataaag atagaagaaa gcaatgtatt
     3001 ggcgcatatt tattttggca aaccagtcaa gcagtacaaa gacaataaaa attatccccg
     3061 aagagatcgt ggattttcag gaaatgttcc cttaaatcct gaccggtctc tgtcgaaaga
     3121 tacactaccg caagaatact cttctcatgg cagcatggac tttcgaacgc ccgcatcgat
     3181 cattcagcgc aagaatggat cagacttact ggatctgaga tatgacagcc attatattac
     3241 agatgggaag ccagagatcg aaggcttgcc tcaaacttat gtgatggaca agtcagaggc
     3301 acaaacgttg gtgatttcac ttaaggatag agaaacagcg atttatttcg atttgttcta
     3361 taccatattt actgatagag cagtgatcac acgatctgta aagataagaa atgaaaccgg
     3421 agaaacaatc aaactagaaa aagcggctag tttccagcta gactttgccc atactaggcg
     3481 atttgatgaa gtcattgctt tgccaggagc acatgtcaat gaaagacaga tttcacggca
     3541 atctgtttta tctggaacaa aagtatttga aagccgtcga ggaacgagca gtcaccatat
     3601 gaataacttt attgctttag ttcatcatca tacgacagaa aatactggag aagcgatcgg
     3661 acttcaattt gtttattccg gcaaccattc tttcgaactt gaaaaagatc agatcaatca
     3721 attgagagtc gtcggtggga tcaacagcca tcgtttttcg tgggaattga atgcaggcca
     3781 gtcttttcaa acacctgaaa tgatcctttc ttactcaagt cagggattga acaaaatgag
     3841 tcagatccac catgagttgc tacgtgaaag aatcgcaaga ggtcgtcatc aatttgctga
     3901 acggccgatt ctggtcaata actgggaagc cacttacttt gacttcaata gtgaaaaaat
     3961 caaggcgatc attgatgaag caaaggagct aggcatcgaa atgttcgtat tagatgatgg
     4021 ctggtttgga aaaagagatg cggacaattc ttcattagga gactggttcg agtatgaagg
     4081 caagttgaca aatggattac gtgaaatagc agattatgct catagcaaag gattgaaatt
     4141 tggtttatgg tttgaacctg aaatgatctc ggttgattct gaactgtatc gaacacatcc
     4201 tgattttctt atgcaggagc caggtagaat gccttctgct tccagatcac aacatgtact
     4261 ggattttaca aggttagatg ttcggcagac gattgagaaa caaatgagaa agattttaga
     4321 tacgattcct cttgactaca tcaaatggga tatgaaccgt tcgctttcag atgtctattc
     4381 gattaccctt gacccacaga gacaaggaga agtagcgcat cgttatatgc tcgggcttta
     4441 tgaactactt gaacatttga caactgatta tccggaaatc ttatgggaag gctgttctgg
     4501 tggaggtgga cggtttgatg ccgggtttat ttattatatg ccgcagtcat ggacaagtga
     4561 taacacggat gcagtggagc gtatgaaaat ccaatatgga acaagccttg cttatccgat
     4621 ttcgtccatc acagctcatg tatcagctgt accaaatcat cagaccgaca gatctacttc
     4681 gttgaaaaca agaggagaaa cagctatgag cgctgttttt ggttatgaac tggatttgac
     4741 aaagctgtct cctgaagaaa aaaagcaagt aaaagaccaa attatatcct atcaaacgat
     4801 tcgtccggtg atccagtatg gccattatta tcgtttggcg agtccttttg aagaaaatat
     4861 tgccgcatgg atgtttgtgt ctcctaaaca agatgaagct attgtgtttt tagggaggat
     4921 actagcttca gctcaaccag cgttccatga agtatatctg atggggttag atgatgaggc
     4981 actttatcag gaacagacct cgaagcggat attttcgggg gccgaattga tgacagttgg
     5041 actttacttc cccgattttc aaggtgattt ccaaacagaa ctgcttcatt tcaaaaagtt
     5101 atgagagaga aggaaaaaag tatgaaagca aatataaaat ggttagatga tccacaagtt
     5161 tttagagtga accaatcaaa tgcacatagc gatcatgttt gttatgcaac aagggaagaa
     5221 gtagaaacca catcctccct taaacagtct ttaaatggat catggcgttt ttgttattcg
     5281 ccaaatcctt cggtacgacc aaaaacattt tataaaaaag attttgatgc ctgttctttt
     5341 gatgaaatca ctgttcctag tcatattgaa ttagcaaatt atgacaagat ccattacatc
     5401 aatacgatgt atccgtggga gggacatgta tttcgccgtc cccctttcac gacaagccct
     5461 gagaaagagg cggaagggat cttttcagct gctaaagaca atccggttgg ttcgtatttg
     5521 acgacttttc gattagaaga aggtctaaag ggaaaacaaa tctcgattca ttttgctggt
     5581 gtagaacaag caatgtttat ttggttgaat ggtcatttta tcgggtatgc cgaagattcg
     5641 ttcactccgt ctgaatttga tttaacacct tatttggaag aagaaaatat tctagctgtg
     5701 gaggtttaca aaagaagtac agctgcattc ttggaagatc aggacttttt ccgatttttt
     5761 gggatcttca gagatgtctt cttagttgct cgaggacaaa accatttaga agatctttgg
     5821 atcaaacctc gcttagatga caaatgtcaa aatggcaaac ttgaagtaga tttgtctttt
     5881 gagcaagtag ccaaaaacag tcagctgaat gtagcgatac tagatcctga tgggaaggaa
     5941 atctatcaac agcaatatcc gttaaaagag caaatgacga ttcgcatcga tggatttgaa
     6001 aatgtggcac tgtgggggca ttttcagccc aatttatata agcttgaatt ggagacgata
     6061 gacgaagaaa aaagagtaac ggagtatatt gactattcct tcggttttcg taaaatcgaa
     6121 atcaaagata aaatcatcta cttcaatgga aaacggatga ttatcaacgg ggtaaatcga
     6181 catgagtggc atccgaaaaa aggacgcgcc atcactatag aagatatgga aaaagatttg
     6241 gctatcataa aaaatgcaaa gatcaacggc gtacggacat ctcattaccc caaccagatc
     6301 acttggtatt atttatgcga ccaagcagga atccatgtga tggcggaaac gaacctcgaa
     6361 tcccatggct catggcaaaa aatgggcgca tttgagcctt cttacaacgt accaggtgca
     6421 gtacctcagt ggcaggcggc tgttcttgat cgagcaaaaa cgaatttcga aacattaaaa
     6481 aaccatccaa gtattctttt ttggtctctt ggaaatgaat cttatgcgga agaaaacatt
     6541 cgacaaatga acgattactt caaaaaagta gaccccgatc gacttgtcca ttatgaaggg
     6601 aattttccaa atccagcatt cgaagattcg atttcagatg taaaaagctg catgtatgcg
     6661 ccaccagaag agatcattga ctaccttgaa aataaagcgg aaaaaccgtt catcttatgt
     6721 gaatatatgc atgacatggg gaattcgtta ggtggaatgg attcttacat gacgttgctt
     6781 gatcgttatg aaatgtacca aggaggctat atatgggatt ttaaagatca ggcgatccaa
     6841 gtgactgatc caatcacagg cagagaagtg atgcgctatg gcggtgactt tgacgataaa
     6901 ccaagtgact atgaattctc agggaatggg atcgtttttg cagatggaca agaaaaaccc
     6961 gccatgcagg aggtaagata ttattatgaa aaatacagta aataaaagtc atatggatac
     7021 ggaaaaagtt gcaatcgtct tcggcgactg tacattaggt gtcaaatcgg ggaatacgca
     7081 ttatattttt tcttatacaa gaggcggact ggaatcgctc aataaaaacg gaaaagaatg
     7141 gctataccgg gaaacacttc cagttttttg gcgagcaacg accgataatg acagaggtag
     7201 cggatttgga tttaaatccg gtatgtggtt aggagccgga ttgtatccaa aagtcaaaga
     7261 aatagaagta ctggtggaca ataaaaagat cgatttgccg attgctccag taaataatca
     7321 atattcaagt aatgaatacg ctgaaacggt tgaaatcatt tttacactgg aaatcaacac
     7381 agtaccaaaa acccaagtga caatttccta ccaaatcgct ggagagggta aaatgcttgt
     7441 caatatgcat tattatggac aaaaaggatt accggaatta cccgcattgg gtttaaggtt
     7501 cattatgcca acgaaagcaa aattttttga gtatgaaggc ctatcagggg aaacatatcc
     7561 ggatcggatg gctggtggtg tcgaaggtat ttataaagtg gcaggactcc ccgtttcccc
     7621 ataccttgtt ccccaagaat gcggcatgca tatggaaacg aaatgggtag aactaacaag
     7681 aaatcaaacg ttggataata cggataaaga tcaagaagat ttttcgttgc gatttgaaca
     7741 gcaggatacg ccatttgctt tttccgcttt accattcaaa gcagaagaac tggaacaggc
     7801 aacacatata gaagaactac cgccagaaag acgaacggtt cttacgattt ttgcaaaagt
     7861 aagaggagtc gggggtatca atagctgggg agcggatgtt gagtcacctt accgaatcag
     7921 cagcgaagag gaccatgaaa tcggctttat tattcagtag caatctttag attattattt
     7981 gaaaaacgga gtttacaaga caaagctgct ttattctata tgtcatgaac aagagagtaa
     8041 aaaagttctc agatactttt gttcatgact attttcttca ataaaaccaa ttgttatttt
     8101 tctgaaaaac aaagttaact atcagaaaat taaaaaaagt ttcataaaaa tgatt
//
LOCUS       DAYYDS010000108         7965 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000108 DAYYDS010000000
VERSION     DAYYDS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.113"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..996)
                     /locus_tag="ACHA02_002487"
     CDS             complement(22..996)
                     /locus_tag="ACHA02_002487"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8670785.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="ACHA02_002488"
     CDS             complement(1076..1951)
                     /locus_tag="ACHA02_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8670786.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="ACHA02_002489"
     CDS             2081..3988
                     /locus_tag="ACHA02_002489"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8670787.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4529..7807)
                     /locus_tag="ACHA02_002490"
     CDS             complement(4529..7807)
                     /locus_tag="ACHA02_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8670788.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2269 a         1667 c         1472 g         2557 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
     4141 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
     4201 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
     4261 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
     4321 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
     4381 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
     4441 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
     4501 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
     4561 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
     4621 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
     4681 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
     4741 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
     4801 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
     4861 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
     4921 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
     4981 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
     5041 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
     5101 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
     5161 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
     5221 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
     5281 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
     5341 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
     5401 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
     5461 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
     5521 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
     5581 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
     5641 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
     5701 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
     5761 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
     5821 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
     5881 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
     5941 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
     6001 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
     6061 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
     6121 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
     6181 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
     6241 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
     6301 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
     6361 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
     6421 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
     6481 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
     6541 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
     6601 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
     6661 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
     6721 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
     6781 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
     6841 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
     6901 caggatcgta ataatatgac tctaaaccag tattcgtttt tttcatctct acgtccagtt
     6961 tagtaatcga gttcgtggcc ggaaacaaat tcccatgacc gtttccgaat atattataag
     7021 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
     7081 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
     7141 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
     7201 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
     7261 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
     7321 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
     7381 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
     7441 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
     7501 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
     7561 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
     7621 ccatatcttc actagttctt ggtacttcct ccattgttga catcccttca atgttcacgt
     7681 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
     7741 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
     7801 ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt attacctaat
     7861 ttatttttac tgtcaataaa aaacaaaatt ataataagtg tattttataa aaagaaaaaa
     7921 tataacttaa gttgttgata ataaagagat ttattaacgt aaaaa
//
LOCUS       DAYYDS010000109         7925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000109 DAYYDS010000000
VERSION     DAYYDS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.114"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            176..877
                     /locus_tag="ACHA02_002491"
     CDS             176..877
                     /locus_tag="ACHA02_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8670789.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1486..2502)
                     /gene="tsaD"
                     /locus_tag="ACHA02_002492"
     CDS             complement(1486..2502)
                     /gene="tsaD"
                     /locus_tag="ACHA02_002492"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8670790.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2499..2963)
                     /gene="rimI"
                     /locus_tag="ACHA02_002493"
     CDS             complement(2499..2963)
                     /gene="rimI"
                     /locus_tag="ACHA02_002493"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8670791.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2970..3512)
                     /gene="rimI"
                     /locus_tag="ACHA02_002494"
     CDS             complement(2970..3512)
                     /gene="rimI"
                     /locus_tag="ACHA02_002494"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8670792.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3496..4218)
                     /gene="tsaB"
                     /locus_tag="ACHA02_002495"
     CDS             complement(3496..4218)
                     /gene="tsaB"
                     /locus_tag="ACHA02_002495"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8670793.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4409..5389)
                     /locus_tag="ACHA02_002496"
     CDS             complement(4409..5389)
                     /locus_tag="ACHA02_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8670794.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5413..6897)
                     /gene="galT"
                     /locus_tag="ACHA02_002497"
     CDS             complement(5413..6897)
                     /gene="galT"
                     /locus_tag="ACHA02_002497"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8670795.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6909..7898)
                     /gene="galE"
                     /locus_tag="ACHA02_002498"
     CDS             complement(6909..7898)
                     /gene="galE"
                     /locus_tag="ACHA02_002498"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8670796.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2230 a         1706 c         1395 g         2594 t
ORIGIN      
        1 acaaatcatt cgcttcaaaa caaattattc aataccattg tatatttttt acataataac
       61 aaattatatt tgtaaagtat taaaacttgt ttctaatgtt ggtatgttaa aactttacaa
      121 attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg
      181 tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt
      241 ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg
      301 acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact
      361 cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac
      421 aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca
      481 agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg
      541 aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca
      601 tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt
      661 attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta
      721 tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca
      781 agtaatcaaa caactacgtg attccgggct gttagattat gacaaaaagc gttttacttt
      841 ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac
      901 cctgataaat atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca
      961 gttttagatc actggacaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt
     1021 cactgtttgc aaaaaatact gttagattaa ctagatgaag cgtcttgtcc tcatccgatc
     1081 tagattattc gatattcctc gacaataaac gattactcag tgagggcaat cagaggaacg
     1141 gataatgata aataagctgt aaaaaatagc aaaataacaa taatgattaa aagtatgtaa
     1201 aaaatagggc tttgacaaaa acgtgtcaca gccctatttc gattaaacgg tgtgtacctg
     1261 tcaactcttc ggtcacaagt cataatgttc ataaacatga ggagctgttt tttgaaattg
     1321 ttccttgtga atcagagtta aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa
     1381 agcttgcata cgcggtaata agaacagcca ggtcaaaaac atgagaagcc gtttttcctg
     1441 ggctgttctt attacttgat gcagggcagc tttttcacaa cctctttatt cagataaaac
     1501 caagcttgga tcagcattca actgatagct agcaaaattt cttttttgca tttccacatg
     1561 tgctgctgct ccaatcattg cagcattatc tccacaaagt cgaagagggg gaatcactag
     1621 ttctacctca gggagtttag ctgccaaagc atcctgcaat ccttcacgca acccttgatt
     1681 ggccgctaca cctccagcta taatcagttg cttgactgga taattttgac aagctcgaag
     1741 tgttttagta atcaatacat cgatcacact tgcttgaaag ctagctgcta aatcattttt
     1801 gtctagatct tctccacgct gttgtgcatt atgcaccaaa ttgataaatg cactttttaa
     1861 tccgctaaaa ctgaaatcat aattatcttc atggatcatc gctcgcggaa aatggtaatt
     1921 atcttttccc tgatgagcta gctgatcgat ttcttttccg cttgggtaag aaagacctaa
     1981 tactcttcca actttgtcat aagcttctcc tgctgcgtca tctcttgtct ccccaatgat
     2041 ttcatatgat ccatcttctt gcatatagac taattctgta tgtcctccgc taacaagaag
     2101 tgccatcaat ggaaactgga atggtttaac cagacgggct gcatagatat gccctgccat
     2161 atggtttaca ggaataagcg gcaattggtg tgcccaagca aaagccttag ctgcagaaat
     2221 accaatcagc aacgagccga caagtcctgg tccataagtg acagcgactg ccgataaatc
     2281 ttctgccgac acaccagctt ctaccagtgc atcttccaag cataaggtga tttgttctac
     2341 gtgatggcgg ctagctactt caggtactac gccaccaaaa cgtttatgac tgtttatttg
     2401 agaagcaacg atgttagata agatctctgt tccatttcta acgaccgcta cactcgtttc
     2461 atcacaactt gattcaatcg ccagaatcaa ttcttcattc atttcatcgt ctccgttttt
     2521 aactttctgc tcataatgat cgcatcttcc gtcggattgc gataatatga tttccgttta
     2581 cctaatatac gaaacttttc tgattcataa agttttcttg ctgcttcatt agatagacgt
     2641 acttctaaaa aaacatgcag cgaatctttt atttgttccg taatcagtaa ttgtaacaaa
     2701 tgccgtgcat gcccttttcc ttgttctgaa actgcaacag caatatttgt gatctcgact
     2761 tcatctatta ctttcgagta tccaataaag ccacagatct ggttatcttc agctaaaagc
     2821 agataatcag agtgaggctg ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa
     2881 ccatgtggat aagcattttc gctaatcatc catacgcatt ctgccaagta cgcttcatca
     2941 aaatcttttt ttctgcaaat caactaatct cactcatttt ctaaaagttt tatcatatcc
     3001 aacgcatctt cttttgtttc agtgtaatat ctttttttga tcgttgttgc ttgaaacccc
     3061 aatttccgat aaagtctttg tgcatcttga ttactcatac gcacttctaa tgacatcgtc
     3121 tcacagcgat tcattatagc aaagttctcg atttcgtcaa taagcagcga accgattcgt
     3181 ttacgctgat atgccggtcg aacggcaata ttcgtgatat ggcaatctaa accgaacgct
     3241 cttgaaccga taaaaccaat cattcttcca ttatcaaaga tacctagata tagatgagga
     3301 agcggtgact tcaattctga tataaaggca ctttttgtcc atggcaattc gccattatag
     3361 acttctcgtt cgacatccag cagctctttg atatcttctg tagtcaattc tcttagtacg
     3421 taacgacgat cgatttgttc gactgttttt tctgtataaa tcattttatc ttgaaaaaag
     3481 cttctggcat acgctctaaa ttttttcaac ataactttcg tctccaggtg tgtgcgtctc
     3541 caaccatttt tcttctgctt ctactcgttt taaatagcga ggaagaaaag cttgtttctc
     3601 tgcaactggt tcaactaaag cacctaattg agccacagtt acaccacttg gaatatccca
     3661 tgctggaact gtattaatca tcgcactagg caatatttcc tttatttcag agaagaattt
     3721 gtgacaatca ctcccgacga aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa
     3781 atcagaagca gaaatatgta catcttcaag tacagtttct aatctatttt ctttccagcg
     3841 gtatgcccca gcgtatacat tttttcttcg agcatcaaac atgggcacaa taatattatc
     3901 cacaccgatg caattagccg cgatggtttt cagactagat actcctacta gctctttatc
     3961 taatgtataa gcaatcgttt ttgctgtcgt tacacctata cgaagccctg tataagaacc
     4021 gggaccatca gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa
     4081 caattggtcg attgcaggca tcagtgttac actgtggttg cgtttgatat ttgtttgtat
     4141 ttgtccaaga atttgttcgt cttctaccac accgatcgcc aaagtttggt ttgcagtatc
     4201 tattcctaat gtaatcatat atttgatcct ttctgctatg acattctcgc cttccattgt
     4261 agcatacttt tttattctga ggatgaaaat accaaaggca aaactagtac aataatacat
     4321 gattatctgc tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc tgcggtatca
     4381 ctccattttc ccacagcttt tccatctttt atttcgttga ttctcgaata atcaactctg
     4441 tttccacaat aatctttctt gaaaccggtg ctttggaatt acttaattga agaatcgtct
     4501 ccactgctag ctctcccatc cattcggtat aaacttttac agtagaaagc gagggcgtta
     4561 catatttcgc tacactgaca tcattgaagc ccatcacaga aatatcttct ggtaccacta
     4621 aacctttttc ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa
     4681 gcgcatcagg aacatgcgga ttcgttttta aatattgctt cactgcttga tatcctgact
     4741 cgacagaaaa atctgcttcg ataattggac cttcgtcgta gcctttttct gccaaaactt
     4801 gttgaaatgc cataattctg ggatctttta gccgctgatg gtttttcttc gtatattctg
     4861 ttccagcaag catagcgata tgttgcgctt tatgggagac aaagtgttca atgacttttt
     4921 gaacacttga cttaaagtcg acaactagag agtcgattcc ttgagaagtg ccgtcagaat
     4981 ctaccaaaag caaggcattt tgggatcctt tcatttctaa taactgagct tcgtcgaatt
     5041 ttcctagtgc gatcgttcca tctgccggaa gatcactaat ggaatcccac gtttctttca
     5101 ataaagtaac gtttgcttct tctgcctttt tttcgattcc taggcgaata gataaataat
     5161 aaagatcttc aagctcttcc gtatcgtcgt accactggat caggcgaagc gtttgcttag
     5221 tatttgctgc atttttacga tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt
     5281 tttgctttgt ggctaagcca acggaaagat caggatcata gttgagaact cttgaaacag
     5341 tagcaggaga aactcctgcc aactgcgcaa tgtctttaat tgttgccatt tgtctctcct
     5401 ccttttttac gtttataaaa tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc
     5461 tcgcttgaat actccagcgt cttccagtac acgtgcaaaa acctgaccta cagatttttg
     5521 tacgacatcg gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta attgttctgc
     5581 ccacggacga tgatattcag ccatctggtt ttcttctcct aataagaaat gctctacttc
     5641 tactagttct ggtttcaaac gaggtggtaa aacagcaagc cccattactt cgatcaaccc
     5701 aatgttctcc tttttgatat gttggacatc tttatgtgga tgaaaaatcc catcaggatg
     5761 ttcttctgaa acattgttgt ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct
     5821 tcgtgcaatt ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag ctctgataga
     5881 tactgtttca tccgaatatg ctttccattt ttccaatact gagttagctg cttcaataag
     5941 caagtttttc tctgttgatt gcaaacggat cacagacatc ggccatttca cgactcctgc
     6001 gatgacttgc ggataatctt ctagtttgat aagtttttct atcggtgctt ttgccatagg
     6061 aaattcatga cgtcctgcct gataatgatc atgagaaaga atcgatcctc caacaattgg
     6121 taagtcagca ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc gaagcagtct
     6181 agcaaatgtt tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg
     6241 ttcattgtaa taagcataag gggaatattg gaatccccag ctttcaccgt ctagattcat
     6301 tcgaataatc cggtgattgg tgcgggctgg atagttcaag cgacctctat atccttcgtt
     6361 ttccatgcaa agcatgcagg taggatagtc tacttttacc gcctcgcgtt ctgctgcgat
     6421 ttgttttgga tctttttctg gcttagataa attgattgtg atttccagtt caccatactc
     6481 cgactctgcc ggaaatttga tgttttttgc gatggctctt gttttgatgt agtcattttt
     6541 cttacttaaa ttaaagaaat actcagttgc ttcctctggg cttttcgaat agtgctgtgc
     6601 aaagaatgca ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa
     6661 aatctcttgt tctgctggca attctgaaag aacaccattt tctcttgcct tagcgacaag
     6721 ctgatccaat aagtctacag attcttcctt aactggtctt acttcaatgc tttctagagc
     6781 atcttctcct accatcccta aaagacgatt ctgtaaatat aagcggtcca tttccatcca
     6841 tccgccagct tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga ttgacatatc
     6901 tcattcccct attcttcata accatttgga tggctgacat gccaatccca tgctgttttg
     6961 ataatcgctt tgatatctgt gtattcaggt ttccagccca gaatctctct cgctttttca
     7021 cttgaagcga ctaatcggct aggatctcca gctcttcttg gtgctatttc tgctggaatt
     7081 tcttttcctg tcacttctct agctgcttct agcatttcct tgactgagta tcctttgctg
     7141 ctgcctaaat tgaagaagtt gctttcattt ccttctttca agtattccaa agctaaaata
     7201 tgtgcagcaa tcaaatcttc tacttgaaca taatctctga tacatgttcc atctggtgta
     7261 tcataatcgt ctccataaac agccaaggct tttctttgac caagagctac ttgtaaaatg
     7321 attgggacaa gatgcgtttc tggtgtatga tcttctccga tagatgcatc tgcttttgct
     7381 ccggctacat tgaagtagcg caaagcgaca tagcgcatgc catatgcttg gtcacaccac
     7441 ttcatcattt tttccatcat caatttactt tcaccatacg gatttttagg attagctggt
     7501 gtatcttctg tgattggaga ctctttcggc tctccataag ttgctgcagt agaagagaat
     7561 acgattttat tcacgttgaa ctcgtgcatg acttcaagta agatttgcat accgtatacg
     7621 ttgttgttga aatacatcaa tggtttttcg acagattctc ctactaaaga gcttgcagca
     7681 aaatggatta ctccttcaat cggttccttt tcaaatacgg aacgtagaaa ttctttatcc
     7741 cggatgtctc cttcataaaa atgagcatct ggatgtactg cttgtttgtg acctgtgagc
     7801 aaattatcta ccacagctac ttgatacccc ttttgaacta gctggtctac agcgtgcgaa
     7861 ccgatataac cggctccgcc tagtactaaa attgtcataa aaaacactcc tttgattagt
     7921 aaaat
//
LOCUS       DAYYDS010000110         7816 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000110 DAYYDS010000000
VERSION     DAYYDS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.115"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..199)
                     /locus_tag="ACHA02_002499"
     CDS             complement(<1..199)
                     /locus_tag="ACHA02_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670797.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="ACHA02_002500"
     CDS             complement(362..1996)
                     /locus_tag="ACHA02_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8670798.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="ACHA02_002501"
     CDS             complement(2000..3373)
                     /locus_tag="ACHA02_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8670799.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="ACHA02_002502"
     CDS             complement(3389..3628)
                     /locus_tag="ACHA02_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670800.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="ACHA02_002503"
     CDS             complement(3810..6485)
                     /locus_tag="ACHA02_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM8670801.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="ACHA02_002504"
     CDS             complement(6668..7591)
                     /locus_tag="ACHA02_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670802.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2287 a         1539 c         1192 g         2798 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ttagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaag
//
LOCUS       DAYYDS010000111         7633 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000111 DAYYDS010000000
VERSION     DAYYDS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.116"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(142..345)
                     /locus_tag="ACHA02_002505"
                     /pseudo
     CDS             complement(142..345)
                     /locus_tag="ACHA02_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            311..508
                     /locus_tag="ACHA02_002506"
     CDS             311..508
                     /locus_tag="ACHA02_002506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670803.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(551..1126)
                     /locus_tag="ACHA02_002507"
     CDS             complement(551..1126)
                     /locus_tag="ACHA02_002507"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8670804.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1281..1807)
                     /locus_tag="ACHA02_002508"
                     /pseudo
     CDS             complement(1281..1807)
                     /locus_tag="ACHA02_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2264..3775
                     /gene="gadC"
                     /locus_tag="ACHA02_002509"
     CDS             2264..3775
                     /gene="gadC"
                     /locus_tag="ACHA02_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFM8670805.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3788..5188
                     /locus_tag="ACHA02_002510"
     CDS             3788..5188
                     /locus_tag="ACHA02_002510"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFM8670806.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5353..6793)
                     /locus_tag="ACHA02_002511"
                     /pseudo
     CDS             complement(5353..6793)
                     /locus_tag="ACHA02_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7186..7633
                     /locus_tag="ACHA02_002512"
                     /pseudo
     CDS             7186..7633
                     /locus_tag="ACHA02_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
BASE COUNT         2400 a         1288 c         1380 g         2565 t
ORIGIN      
        1 tttaattttg attttcaata aaaatatatt acaataataa tcgtaatagc taatgaaaca
       61 atttctcaat ctttttacca ctccaaacag ggagcgtcaa taattttgtg taaataactc
      121 gtccttctgc aaaataatgg gttactcagt aaacattgaa gctaatgtat ccgtcacttg
      181 ttggaagcct ttatggcttc tatttaagaa tttttgattg tatgtatcaa agatggatac
      241 taggaaacgc tctagtgatt cttcattttg aaactgctct tttctgcggc tgtatttctt
      301 gatttgttta ttgaaagatt cgattgtaga accagaagga atggagaaca cttttgaact
      361 ttacaacaaa actactgttg ataaattaat tccaatttta gttccaactg atttaaatgg
      421 tcatgatgtt ttacttcgtt ttaacggatt tttaggtgag gattatagat acctaaaaat
      481 ttctgatcct caacctttaa cagaataata aaaaacgtgt aatcaattat ttgattacac
      541 gttttttatt tcaatcttcg ttgtagtagt ggttgcgaca taccgaaagg taatcttcat
      601 tacctcctac tacaatctgt tcacctgaat aaactggaat accatcaaca atacgagcat
      661 tcattacagc ctttttattg caaaaatggc aaatagtttt aatttcctca attttatcag
      721 caaatgtcaa taatgcttcg gatcccttga agagattatt ttgaaaatca ttcttcaatc
      781 catagcaaat tactggtact tcctcattca ttacgataag tgatagatca ataatattct
      841 ctctacttaa aaattgagac tcatcgacca aaatacaatc aacttttccg tactgatcaa
      901 taatctccac taattctccg tacacatcca tttcagaagt aattgcaata gctggtttcg
      961 aaacacctat acgactctca acttttccaa atcctgatcg agtatctatt gcgcttgtca
     1021 ttatgataac tctcttccct tgttcttcat aattatgagc cactttcagt agctcaatac
     1081 tctttccact attcattgtt ccatatctgt aaaataattg tgccattaaa tttattccct
     1141 tcttttacaa ttcaattatt ccttaattcc atctgctttt atgcagctgc tcgtttattc
     1201 cttgatatgt ctataacatc tactatccct aaaaccaatt tataagccat tagagatggt
     1261 attcctgaac atctctcata ttgttttttg agtggtatat catctgaaaa actgaaggaa
     1321 tcatgaaaat agcgacatac ttatcattta gagttctaac tttaaatgca tttacttctt
     1381 cagtcagcac tttagtcatg ttggacatgg tttggggcgt gtagtgatgc ccatacattt
     1441 tctcaatcaa gtctgcgatt tccgacatgg taataccttt tcggaagagg tgaatgacgg
     1501 tctcctctaa tgtgtcattc gtccgtctat aagcaggaac agtctgttgc ttgaactcgc
     1561 cattgcggtc gcgcggaatc tggagatgaa gttccccata ctcggtcttg accgtacggt
     1621 cataggagcc gttacgagaa ttacccgtgt taaaaccaat gcgatcgtac ttttcgtaat
     1681 cgaggaaagc cgttaactcc gttttgagaa gcgtattgac agctttttcc aaatggacac
     1741 ggaataatcc attcaaatcg cctttgttcg ctagagtctt taggatttct gtagtaaaat
     1801 cgttcatatg gaagtcctct tttctgtgaa tgtgttgtgg taactctatt ctacagaagg
     1861 gacttccttt tttctattta cacaaaatat tttacactct caacaaaagt atcttcgatt
     1921 aatacttgct caagttatgt gcagctacga aaaagtaggt accgaggtac tttctttttc
     1981 gcagcttttt ttggacttat gagaagtaca ttcgctggaa gaatacgata taaaacgagc
     2041 tttttcacta aagatattaa tagagtgaaa gaaacaagct tttgttgtca gttttttcac
     2101 ttttctaaaa atgaaaataa aaaattctgg aagtggagaa gaattttgct tgctatactt
     2161 tgtcttgtag aaaagaggta gacaaaataa tgcaagctca aataagcgat tttccataac
     2221 cttggtagga atgacaaatt aaataggaaa gggaagtaat aatatgaata aaaaaaagtt
     2281 atctctattc ggtttttttg ccctgacagc ttccatggtt ttgactgttt atgagtatcc
     2341 gacctttgca acatcacaat tacatttggt tttctttctt cttttgggag gtattttatg
     2401 gtttctacca gttgctttat gtgctgcaga aatggcaaca gttgaaggat ggaaagatgg
     2461 tgggattttc agttgggtta gtcaaacttt aggagaacgt tttggttttg cagctatttt
     2521 tttccaatgg tttcaaatta cagtaggttt tattacaatg atctacttca tactaggagc
     2581 gctgtcgtat gtaattaatt ttccagagct taatacagat ccatttatca agtttattgg
     2641 tgtattagtc attttctggg tactgacttt ttcacagttg ggcgggacca atcgtaccgc
     2701 taaaattgct aaagcaggat ttgtcattgg aattgttatt ccttcgatca ttctctttgg
     2761 tttagcagca gcctacttta tcggcggtaa tccgattcaa atggctatta atgcgaaagc
     2821 ttttattcct gacttttctc agctttcaac tttagtagta tttgtatctt ttatattggc
     2881 ttatatgggc gtagaagctt cagcatcaca tattaatgag ctggaaaatc cccaaagaaa
     2941 ctacccattg gcaatgtttt tactagtgat tttggccatc gccttagatg caatcggcgg
     3001 tttctcagta gcagcggtta ttccacaaca tgacttgtct ctcagtgctg gtgtaatcca
     3061 aacatttgaa acgcttattt tacattttaa cagtcattta cattggttgg taaaagtgat
     3121 tgctctcatg attgcctgtg gcgttatggg tgaagttagt tcttgggtag ttggtccatc
     3181 acgagggatg tttgcagctg ctcagcgagg tctgttacca gttttcttcc ggaaaactaa
     3241 taaacaagac gttccagttc cgttgattat gatacaagga attatcgtta ctatttgggc
     3301 agcagtctta acttttggtg gaggaggcaa caatttatca ttcttagtag caatctcctt
     3361 aactgttgtc atttatctcg taggatattt actatttttc ctaggttact ttgtattagt
     3421 ttttaagaaa aaagacttaa aacgaactta caatgtacca ggaaaaactg ttggcaaaac
     3481 gattatcggt ggaattggtt ttattttatc agtgttcgca ttgcttatct cttttgtacc
     3541 accagcttca attgcagcta gtgagcaccg aacataccaa atcatactac ttattagctt
     3601 tgtagtgact gttcttttac cttttatcat ttatgaagta catgataaaa gtgctcacac
     3661 aactattgag gaacctactc acgtgaaagc tgaagacgtt aatcccgctg tttatccagc
     3721 tgctagagga gagcaccacg ttacaaaaaa tgatgaacat attttaaaac attaggagga
     3781 attcattatg ttatacggaa aagataatca agaagaaaaa aactatttgg aaccaatttt
     3841 tggctctgca agtgaggatg ttgacttgcc taaatataag ttaaacaaag aatccattga
     3901 accacgaatt gcttatcaat tagtacaaga cgagatgttg gatgaaggaa atgcgcgatt
     3961 aaacttagct actttttgtc aaacgtatat ggaacctgaa gcagtgaaat tgatgaccca
     4021 aacgttagaa aaaaatgcaa ttgataaatc agaataccca cgaacaacgg aaattgaaaa
     4081 ccgctgtgta aatatgattg ctgatttatg gcatgctcca aataatgaaa aattcatggg
     4141 aacttcaacg atcggctctt cagaagcctg catgctgggt ggtatggcca tgaaatttgc
     4201 ttggcgtaaa cgtgctgaaa aattaggtct tgatattcaa gcaaaaaaac ctaacctggt
     4261 gatctcttct ggttaccaag tttgttggga aaaattctgt gtatattggg atgtggaact
     4321 gagagaagtc ccaatggatg aaaaacatat gtcaattaat ctagatactg tcatggatta
     4381 tgtggatgag tacacaattg gtattgtagg tattatgggt attacttaca ctggtcgtta
     4441 tgatgatatc aagggtctga atgatttagt tgaagctcac aataaacaaa ctgactataa
     4501 agtatacatt catgttgacg ctgcatcggg tggcttttat gcaccattta ctgaacctga
     4561 tctagtttgg gattttcaat tgaaaaatgt tatctcaatt aattcttcag gtcacaaata
     4621 tggtttggta tatccaggtg tgggttgggt cttatggcgt gaccaacaat acttaccaga
     4681 agaattagta tttaaagtta gttacttagg tggagaaatg ccaactatgg ctatcaactt
     4741 ctctcatagt gcagcacaac taattgggca atactacaac tttgtacgct atggctttga
     4801 tggttatcgt gatattcacc aaagaactca tgatgttgct gtttatttag ccaaagagat
     4861 cgaaaaaact ggtatttttg aaatcattaa tgatggatca gaattgccag ttgtgtgcta
     4921 taagctgaaa gaagatccca atcgcgaatg gacactatat gatttatctg atcgtctgtt
     4981 aatgaaggga tggcaagtcc cagcctaccc attacctaaa gacttggatc aattaattat
     5041 tcaacgctta gttgttcgag cagactttgg aatgaacatg gctggtgatt atgtacaaga
     5101 tatgaaccaa gcaattgaag agttgaataa agctcatatt gtttatcata aaaaacagga
     5161 tgtgaaaacg tacggcttta ctcactaaaa ttcttcagag aaatataaaa gaagttaatt
     5221 aactaataaa aggagttgtg attaccacac agctccttaa atctatttga ataaatatat
     5281 gatattcatt tgttacactc tcaatcttag ctgttgttct attcatcatt actcttattg
     5341 taagtagttc agtcaaattt gcttgttcaa ttgatcgata acagtatgaa ttgtttgaaa
     5401 agtaatcgga tatataattg ttttctcact acctaagcct tcttggtata aagaactatc
     5461 gcaaagtata atccgctgag tacaaaagaa agtgtcgtct actatttctc taagttgttt
     5521 acaaatgata ttactaatag aaaagtgttc tcccaaaatg tcttctaact gttgtcgata
     5581 gtataaatac ctaaagtctg acggagctac tatgaacact tcaattggag atttccgttt
     5641 atgctcattt aaaagactta aactaatggt tagtttacga atagaattct cattccaacg
     5701 cagaaataaa ttgttttgtt ttcgccattg ttcaagaatt tcatgaactt ctaagtacaa
     5761 agcgcgttgc tcttcattta acaagtaagt cttatcaggt tgaaatatct gaatatcagc
     5821 ccaaagtgag cggataaacg ggagaagtgc tttatcaaaa agtatctgat tttcttcaga
     5881 tatattcaga gataagataa gtttttgact gagctcaatc accaaagggt tatctgctac
     5941 atggttttga taaacaacgt tcaagtcttt ttgtaacaat tcaaagttat cgcatagata
     6001 atttctagag ttgaatagaa agtagatata aaggcattca tcttcgtcat gacataaaga
     6061 gaaagaaagt tgctctaatc ctttctgtat tagctgaaac aagggagtat gtcgggcaac
     6121 tcttttttct tcctgactaa aatatattgg gttagtctta gatctctgca aaccgatagc
     6181 gatacctaat aaaataaatt gtttttcaac tgtagggtaa cttcttgaaa aaaaatgttt
     6241 ttctacataa tcaatcaatt gttgaccgct ttgaataatt gatttattta gctggatcaa
     6301 ctctgtccga tttgtctcat agattatcgt tagtaacaaa cttcgataat ccttttcagg
     6361 aatttcaaaa tggccatcag catttaagta ctgcatttgt ttaaaaaaat taatcaactc
     6421 atttcgtaag ttgtaaactt tgcttaacga aatgaaggta gtatcagcta tattactcca
     6481 attgtgttcc cctgataaat aatgtcctag tacattgaaa aagtcagagc gctgatagat
     6541 gagttgagtg agtgccaacc tcgaaaacga agagtcgaat gcgactgtaa agctacttct
     6601 agatttctcc actgaaataa tttgctgatg gagtatgtcg gtaatgcatt ccaagtcgtt
     6661 agtgacagac agtgagtggg caaacgttca attgatctgc tatttcgcga atctgaatgg
     6721 tccctttttc gtgtaattgc tcacaaatgt aaacttgacg aaaaatactt ttctccagat
     6781 aggtttccaa catagtactc ctcctagtca ttttatacat aactattata actctaccaa
     6841 tcggaatagt cctgtaatat attgaatcaa aaatataaat tataaaataa ttagatgggg
     6901 tatttggttt aagtatccct tatatagtaa taaatttata ggtgtgattc tcgaattaat
     6961 acccgaaaat gtgagagaat ttttatggat tttttaggat acaaattaat tatttttgct
     7021 tttatcctga ttaccagtcc agtactgtcg gtatcccatt ttgcagcaaa atcatgttga
     7081 taaagtgaca agaagactga tgagatatat tatccaaatt gagaagataa ttaatcggat
     7141 tcttgagtat accctacttt ttaagaaagt agggtataca agcgtatgaa taaagacata
     7201 actttccctg tgatggacgt tcggcttgtt ccaattacca aaatacaggc gaacgattat
     7261 aatcccaatc gagtagcgtc tccggaaatg aacttattgg agctttctat cattgaagat
     7321 ggctttactc aaccattagt ttgttattac aaggaaaaag aagagaagta tatcttggtt
     7381 gatgtgcttc ataggtatcg tgttgctaag gaaagactca acttaaaagt tgtaccggta
     7441 acagttattg ataaacccat ataagaacgt atggcttcaa caattcgaca caatcgtgca
     7501 agagggaccc atgaaataag aggaatggaa attgtcatcc aaagattagt aaaaaatgga
     7561 tggtcagaca agagaatatc caaagaatta ggcatggaca ttgaagaaat ttttcgcttt
     7621 aagcaagtgt ctg
//
LOCUS       DAYYDS010000112         7600 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000112 DAYYDS010000000
VERSION     DAYYDS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.117"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACHA02_002513"
     CDS             complement(138..428)
                     /locus_tag="ACHA02_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670807.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACHA02_002514"
     CDS             complement(546..806)
                     /locus_tag="ACHA02_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670808.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACHA02_002515"
     CDS             complement(943..1356)
                     /locus_tag="ACHA02_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8670809.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACHA02_002516"
     CDS             complement(1812..2297)
                     /locus_tag="ACHA02_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670810.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACHA02_002517"
     CDS             complement(2436..2651)
                     /locus_tag="ACHA02_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8670811.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACHA02_002518"
     CDS             2875..3675
                     /locus_tag="ACHA02_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8670812.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACHA02_002519"
     CDS             3694..5562
                     /locus_tag="ACHA02_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8670813.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACHA02_002520"
     CDS             5555..6046
                     /locus_tag="ACHA02_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8670814.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACHA02_002521"
     CDS             6034..6588
                     /locus_tag="ACHA02_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8670815.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="ACHA02_002522"
     CDS             6606..>7600
                     /locus_tag="ACHA02_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8670816.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAYYDS010000113         7546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000113 DAYYDS010000000
VERSION     DAYYDS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.118"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..437)
                     /locus_tag="ACHA02_002523"
     CDS             complement(<1..437)
                     /locus_tag="ACHA02_002523"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8670817.1"
                     /translation="MFQILKLSKNEIRHSNFLAFLFAPNETHNLSDEFFKMFLKRYID
                     SNDDTKAAINYFDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQ
                     LNKYQIYIERNYSNYKKIFVFLTPDGNEPTNPSWGIITYKNIV"
     gene            complement(401..526)
                     /locus_tag="ACHA02_002524"
     CDS             complement(401..526)
                     /locus_tag="ACHA02_002524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670818.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            complement(729..1208)
                     /gene="rlmH"
                     /locus_tag="ACHA02_002525"
     CDS             complement(729..1208)
                     /gene="rlmH"
                     /locus_tag="ACHA02_002525"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8670819.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1636..2925
                     /locus_tag="ACHA02_002526"
     CDS             1636..2925
                     /locus_tag="ACHA02_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8670820.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2964..3569)
                     /gene="ytpR"
                     /locus_tag="ACHA02_002527"
     CDS             complement(2964..3569)
                     /gene="ytpR"
                     /locus_tag="ACHA02_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8670821.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3774..4238)
                     /locus_tag="ACHA02_002528"
     CDS             complement(3774..4238)
                     /locus_tag="ACHA02_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8670822.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(4260..4580)
                     /locus_tag="ACHA02_002529"
     CDS             complement(4260..4580)
                     /locus_tag="ACHA02_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8670823.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4724..5803)
                     /gene="pepA"
                     /locus_tag="ACHA02_002530"
     CDS             complement(4724..5803)
                     /gene="pepA"
                     /locus_tag="ACHA02_002530"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8670824.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            6003..6353
                     /locus_tag="ACHA02_002531"
     CDS             6003..6353
                     /locus_tag="ACHA02_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8670825.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            6576..7523
                     /locus_tag="ACHA02_002532"
     CDS             6576..7523
                     /locus_tag="ACHA02_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8670826.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
BASE COUNT         2267 a         1443 c         1356 g         2480 t
ORIGIN      
        1 tcaacgatat tcttgtaagt aattatgccc caactaggat ttgtgggttc atttccatct
       61 ggagttaaaa aaacaaaaat ttttttatag ttagaataat tgcgttctat atatatttgg
      121 tatttattaa gttgccctcc agactccgaa gacagaattt tattttcgat gcacaccact
      181 attttatttt tttccgatac caataaaata tcaatattct tattttctct gtaaacaatt
      241 aaatcttcat agctattaag taaagcatca aaataattga tagcagcttt ggtatcatca
      301 ttagaatcta tatacctttt caaaaacatt ttaaaaaact catcagataa attgtgggtt
      361 tcatttggag caaacaaaaa agctaaaaaa ttactatgtc ttatttcatt tttagatagc
      421 ttaagtatct gaaatatatt tgttttccca gtccatttag atagttttct tcaatatcat
      481 ccatatcctc taatagattt tttaaacact ttttttcatc tatcatgttt tttctccttg
      541 ccattattct aactaaaatt ttacgtattt gtttctatgt ctacattatc tttttaacag
      601 tatctatttt tcttttgcaa cttacttggc taccaaagca aataaaaaag ttatttataa
      661 atttctccct accactacct attgctatta tttaaaatag ggataatttt ttctcttaaa
      721 aaatatcatt atttgtggta tggagccccc gcattgatcc ggaaggcacg gtagatttgc
      781 tcgacaagga tcagacgcat caattgatgt ggatacgtca tttttccaaa agagatctga
      841 gcattgcttc ttttcatgac ggcttcgctg agtcctaaag agccgccgat aacaaaaaca
      901 aggtggcttt ttccttggat acctaattgg tcgatttgtt tagcaaaaga ttcacttgta
      961 ggatttttcc cttcaattgc taaggcatag acgtattctt gttctttgat tttcgctaag
     1021 atccgttccc cttctttttc ttttacttgt ctcatttgcg cttcgcttaa attctccggg
     1081 gctttttcgt caggaacttc gatcaattcg atttttgcat aggcattcag ccgtttgaca
     1141 tactcattga taccttggat caggtatttt tcttttaatt ttcctactga gataattttg
     1201 atattcatgt tttctctcct ttggtcttca tgctcacttt gtttattata atcaattttt
     1261 tcgtctatat gtttattttg gctatttttg ttaaaaagat ttccccaaca gttatgcaca
     1321 acatccacag gtttatccac agaaaacagt gttattttac cttatttcat cacattcatt
     1381 aggcaaacat acgttttata agaatttatc cactttatac acaggctgtg gatatgtttt
     1441 atcccttatt tttccaatcc tatccgcgta gttttacaca gtatctgtgg aaaaagaaaa
     1501 tcaaatattt tcatctgaaa acaatgattt ttcctaaagt ttttcataaa aagttcacag
     1561 tctcaaagta gactaaagtc aacaaataag actatactaa cagtagataa atgaatgtaa
     1621 ttggaggagg aacacatgga tagaaaaaat gtgacaccaa agatgaaaaa aagtaaaaat
     1681 aatagcctct ggcgtaagtt aggtctgggg cttgttggag ggatcgttgg aggactactg
     1741 acagctggca ttttttatgc agcgatgggc actggcaaca atagttctac tgctacaagc
     1801 ggcaatcaga attctgccgg tgaaacagtt gtggaaaatg tcaaagtcaa tgtagattct
     1861 gatatcacca aagcggtaga taaagttcaa gacgctgtcg tttctgttat caacttacaa
     1921 agtcaaaacc aatcaagcgg ttttggcgga ctattcgggc agcaagaaga aagtagcagc
     1981 tcagatgata gtaatttgga agcttatagt gaaggaagcg gcgtgatcta caaaaaagac
     2041 gggaatactg cttatgtagt cacgaataat cacgtagtag atggccagca aggtcttgaa
     2101 gtcttgatga aagacggtac gaaggtaaaa gctgaattag ttggaacaga tgcttattca
     2161 gatttagcgg tgttgaagat caattcagac aaagtcgaaa cagttgcctc ttttggtgac
     2221 tcaagtgctc tgaaggttgg tgagcctgcg attgcgattg gttccccact aggatctgaa
     2281 tatgcaaatt ctgtcacttc aggaatcatt tcttccttga accgacaagt gacaagtacg
     2341 aatgaatcga acgaaacagt caatatcaat gcgatccaaa cagatgcagc aatcaaccct
     2401 ggaaattctg gtggtccttt agttaatatc gaaggacaag ttatcggtat caactcaagt
     2461 aagatcgcaa gtacttctga atctacttct aatgtaagcg tagaaggaat gggatttgct
     2521 attccaagta acgatgtagt gaatatcatc aatcaactgg aaaaagacgg aaaagtgaca
     2581 cgtccagctc taggtatcac aatggtcgac ttatctgccg tttctactca gcaacaagaa
     2641 caaatcttga agattccttc atctgtaact aacggtgtca tcgtgacaaa tgtccaaaca
     2701 gctacaccgg ctgaaaaagc tggattgaaa caatacgacg tcattactaa gatcgatgac
     2761 aaagaagttt cttctggtgt agaacttcaa tctgctttgt ataagaaaaa agtgggagat
     2821 tctgtcaaaa tcacttacta tgaaggctcg aaagaaaaaa caactactgt tgaattgacg
     2881 atcgatcaat cggcattaaa acaaaatcaa aatagtgaaa attaataaaa aagaggttgt
     2941 gacaaagact tgtcacaacc tctttatttt ggaaatgctt ctccgactac ggcatcctca
     3001 ggcaatacta agattccttt ttttgctgga gcatttggca aatgcaattc acgagcggaa
     3061 cagatcattc catagctatc tacccctcgc aaagttcctg gccagatcat cattccatct
     3121 ggcatcatag ctcctggttt tgcaacgaca actttttgtc cagcttcgat attgggtgca
     3181 ccgcaaacga tttgcaatac tgtaccattg tctacttctg tctcagtaat cgataaatgg
     3241 tcagaatcag gatgtgggac gcatgttttt acgtacccga caacgatctt cggttcatga
     3301 tcagcttcaa gctgttcttc gaatcctgct tctttgatca atcgattcaa tttcgctaat
     3361 tcctcatctg ttaagcttac ttggccgttc ccttcgattg ttagatgatc ggagatatgg
     3421 aaaaagttcc aagcaacaac ttgaccttgt tcatctttga tccgagcaac attccatttg
     3481 cgttcaacag tgttttctgt tcctttatcg tcggctataa taaccatcaa ggtgtctccg
     3541 acgtgtgctt tgttataagc aaaaatcatg ttctttcctc cattatcctt tgctgagatt
     3601 tatcttaaca aaaaattgca ttcactgcat gaaatttttt tcatgcttta tttccgaaga
     3661 agcctcttct ggaacaccat ttattcgtaa caagggactg tgacaagact tttgtcacag
     3721 tccctttact gttttagtta attgattaaa aaggtcgtac aagttccact tgcttaatta
     3781 cttggctgtt cttcttcgtc agaaacgatc aagacatcac aaggtgaatg acgtatcacg
     3841 tagctggcta cacttcctgt cataaagcgt tcgactgcat ttagacctga ctgcccaacc
     3901 atgattagat cgatctcata tttttttggc aatttgactg ccatggattc acgaacagaa
     3961 ccatatgcaa taattccttc tatatttgtg taatcaacag atttcccata ttctttacat
     4021 tcttcgatca tttccttggc tgtttcagtt tgctgatcga ctagattttg atcaataggt
     4081 gcatagccca taaagttata aaattgctga tcgattacgt ttgctacata tactttccca
     4141 ttattacggc gagccacttc taccgctttt ttgaaagcag caagtgcttg ttcactaccg
     4201 tcgataccta ctaaaatatt cttatattct tgattcatct tgacccctcc taacagatgt
     4261 cagccttttg ttacactttc taggaacgat tcgatttcct gttttgtttt acgatcttta
     4321 ttcactagac gacctaattc ttttccgtct tggatcacga caaaacttgg gataccaaaa
     4381 atgttccatt cagcggctac atctatgtac ttgtcacggt ctacttcaat aaattgccaa
     4441 tcagaaaagt cattttcaat ttcaggcatt tgcggtttga taaaacggca gtctccgcac
     4501 cagccggcag taaagaaaaa tacactcttt ccagtttcta cgtagcctgc taattcttct
     4561 aaactatttg ggataatcat tctttgtcct ctactttctg tttatgtatt tttcaattaa
     4621 attatacacc tttctttcga actaacaaac attgatcctc tttttataaa aaaaatctgg
     4681 caaaaagcaa tcttttgcca gattttatag tttcactatc gcattatttt ccgtatacga
     4741 tcgtatttac tgttgattta tccaaacctt tcagtgtttg aatcaacatt tctcgtgcag
     4801 cttcaaaatc gcgaatactg aacatcgttt gatgcgtatg gatataacgt cccactacgc
     4861 caatcactgt acttgggatt ccttcgtttt gtgtatgagc agcacctgca tctgtaccgc
     4921 cttttgaaac aaagtattga taagggatat tgtttgtttc agcgatatcc agcaagtact
     4981 cgcgcaaacg aggaagcatg atcaaacctg gatcttggat acgcatcaat gtaccttctc
     5041 ctaaatgtcc gaatgtgcca tttttcgtca ctgtatcatc tgctgcagag cagtctactg
     5101 caaagaacag atctggcttg aatttattga cagaagcttt agaaccacgc aagcctactt
     5161 cttcctggac attcgcgcct gcaatcaatg tatgacctaa tgtttcattt tgcaacgctt
     5221 ctaaagcttc taataccatt gtacagccat agcggttgtc ccatgattta ctgataatat
     5281 tctttccgtt ggctgttttg atcgtttcag cgtaaggaac gattgtgtct cctggaagga
     5341 caccgtaact cattgcctct tctcttgatt caaacccagc atcgaacaaa atatcggtta
     5401 cttctactga tttttgtccg cttgttcctc gcaataaatg tgggggaacg gaagaagaaa
     5461 tacatggata attcttacca gtagaggttt taagtgtaaa acgctgagca gaaacaacgt
     5521 aaggattcca gccgcctaga ggtacgactt taaaaagccc gttttcttgg atttgtgtca
     5581 gcatgaatcc gacttcatcc atatgtgctg cgaccattac acggggtgca tccgcttctt
     5641 catgatggcg cagaccaaaa atgccgccca aaccatctaa ctgtaactca tctaccagcg
     5701 gttccatatg actttccata taagcgcgaa catcttgttc aaaaccgctt gttccttgta
     5761 attctgttaa ctctttgatt cgttgaaatg ttttttcttc catatgctat tcgtctaaat
     5821 ttcagactaa actcctctca gacttatatt gatctgcgga taaaatatga ttttttctgc
     5881 agaccgatcg tatttattat agcttatttt ttctggtagc ggttatttat ttcaaagaaa
     5941 atatgagagt gtggtaaaat aagcacaagg agtaacaagt gagaagaagg aggtatgtcc
     6001 acatgtatga agaaagcgaa ctaagctatt ttaaaggcgg gttagcagtt ggaatcggtc
     6061 taggattatt gagcggggta gcttcaacga tttggtatca tcgcaaaaaa gtattgtcag
     6121 cagaccaagt acttgaaaat atcaagtcat cttttctaaa agaaggtgca atcgaaggtt
     6181 cttggatctc ttttgaaaag gaacctatta ggaaattcgc cgttcggtcg agtgcttatc
     6241 ggggaggtct ttctcgaata gaagatggcg aaactgttta ttatgaattt ttagcagatg
     6301 ctcatacagg aaccgtatta gaaatttcaa gaaaaaaagg tgcaacagat taatacgtca
     6361 aaaaagtgag agaacgaata cgcacccact attcaaaaga gaggtatcat gtccttcttt
     6421 tgtagtgggt ggttttttag atagagcttt ccaaacatat attccagtat tttcttttta
     6481 aataaatgat atttagcggg aaaaactagc atttcctttg attatgaggt atttttaatt
     6541 tagcttttgt tttctatagt taaggagagt gaaaaatgga acaacaagaa aaaattgctg
     6601 ttctgattcc ttgttacaat gaggaagcaa caatttcaac agtcattgca gactttaaac
     6661 gagaattacc agaagcagat atttatgtct atgataataa ttcgaccgat cgaacatacg
     6721 aattggccgt tcaaggtgga gcaatcgtaa aaaaagaacc tcgtcaagga aaaggaaatg
     6781 tgatccgcca aatgttcttt gatatcgatg ccgactatta tttaatggta gatggcgatg
     6841 atacttatcc tgcagaagct gtacatggtt tgctcgagaa attacgttct ggtgaagcag
     6901 atatggtcat tggtgatcgc ctatcaaatg gaacctactt tgatgagaat aagagacctt
     6961 tccacgattt tggaaataac cttgttagaa atacaattac tagaatgtat aaaacaaaaa
     7021 ttcttgatgt catgaccggc tatcgaggat tcaatcgtat ctttgtcaaa agttttccta
     7081 ttatgagttc tggttttcaa atcgaaacgg aattgacgat ccatgcttta gataaaaaat
     7141 tcaagttcgt tgaaatccca atcgattatc gtgatcgtcc tgaaggcagc gaatcaaaat
     7201 tgaatacatt ctctgacgga ttcaaagtga tcatgatgat tattaaaatg tttaaagatt
     7261 ataagccttt gcttttcttt gggatttgga ccatcttctt ctttttattt ggattggtca
     7321 cggggattcc tgtgattcgc gaatatatgc tgacaagctt tattacacgc atcccttctg
     7381 cgattttaag tactggattg atgactttag cacttctttc attagtaaca gggttgattt
     7441 tggatacagt cgtcacgaac gctaagaaag aatatgaatt gaatctatat catgtttatc
     7501 atgaatacca cagtagaaag taaaaaaagg atcaagactg caaaaa
//
LOCUS       DAYYDS010000114         6903 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000114 DAYYDS010000000
VERSION     DAYYDS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.119"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHA02_002533"
     CDS             complement(<1..182)
                     /locus_tag="ACHA02_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8670827.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHA02_002534"
     CDS             517..951
                     /locus_tag="ACHA02_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8670828.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHA02_002535"
     CDS             974..1294
                     /locus_tag="ACHA02_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670829.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHA02_002536"
     CDS             1435..1626
                     /locus_tag="ACHA02_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670830.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHA02_002537"
     CDS             complement(1697..1954)
                     /locus_tag="ACHA02_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8670831.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHA02_002538"
     CDS             2112..2375
                     /locus_tag="ACHA02_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670832.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHA02_002539"
     CDS             2399..2587
                     /locus_tag="ACHA02_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670833.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHA02_002540"
     CDS             complement(2670..2855)
                     /locus_tag="ACHA02_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670834.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHA02_002541"
     CDS             3070..3441
                     /locus_tag="ACHA02_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8670835.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHA02_002542"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHA02_002542"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHA02_002543"
     CDS             complement(3709..4137)
                     /locus_tag="ACHA02_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670836.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHA02_002544"
     CDS             complement(4197..4955)
                     /locus_tag="ACHA02_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8670837.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACHA02_002545"
     CDS             5250..5447
                     /locus_tag="ACHA02_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670838.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACHA02_002546"
     CDS             5553..6035
                     /locus_tag="ACHA02_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670839.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACHA02_002547"
     CDS             complement(6093..6689)
                     /locus_tag="ACHA02_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8670840.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2339 a         1061 c         1355 g         2148 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttgaaaaa
     6901 aac
//
LOCUS       DAYYDS010000115         6872 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000115 DAYYDS010000000
VERSION     DAYYDS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.120"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(198..656)
                     /locus_tag="ACHA02_002548"
     CDS             complement(198..656)
                     /locus_tag="ACHA02_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8670841.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(669..2000)
                     /locus_tag="ACHA02_002549"
     CDS             complement(669..2000)
                     /locus_tag="ACHA02_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8670842.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(2011..3453)
                     /locus_tag="ACHA02_002550"
     CDS             complement(2011..3453)
                     /locus_tag="ACHA02_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8670843.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3440..4204)
                     /locus_tag="ACHA02_002551"
     CDS             complement(3440..4204)
                     /locus_tag="ACHA02_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8670844.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4308..5183)
                     /locus_tag="ACHA02_002552"
     CDS             complement(4308..5183)
                     /locus_tag="ACHA02_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8670845.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5438..5522)
                     /locus_tag="ACHA02_002553"
     tRNA            complement(5438..5522)
                     /locus_tag="ACHA02_002553"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5486..5488),aa:Leu,seq:caa)
     gene            complement(5540..5611)
                     /locus_tag="ACHA02_002554"
     tRNA            complement(5540..5611)
                     /locus_tag="ACHA02_002554"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5577..5579),aa:Gln,seq:ttg)
     gene            complement(5621..5693)
                     /locus_tag="ACHA02_002555"
     tRNA            complement(5621..5693)
                     /locus_tag="ACHA02_002555"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5658..5660),aa:His,seq:gtg)
     gene            complement(5755..5839)
                     /locus_tag="ACHA02_002556"
     tRNA            complement(5755..5839)
                     /locus_tag="ACHA02_002556"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5803..5805),aa:Leu,seq:caa)
     gene            complement(5860..5930)
                     /locus_tag="ACHA02_002557"
     tRNA            complement(5860..5930)
                     /locus_tag="ACHA02_002557"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5896..5898),aa:Cys,seq:gca)
     gene            complement(5976..6047)
                     /locus_tag="ACHA02_002558"
     tRNA            complement(5976..6047)
                     /locus_tag="ACHA02_002558"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6013..6015),aa:Gln,seq:ttg)
     gene            complement(6059..6131)
                     /locus_tag="ACHA02_002559"
     tRNA            complement(6059..6131)
                     /locus_tag="ACHA02_002559"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6096..6098),aa:His,seq:gtg)
     gene            complement(6134..6207)
                     /locus_tag="ACHA02_002560"
     tRNA            complement(6134..6207)
                     /locus_tag="ACHA02_002560"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6173..6175),aa:Trp,seq:cca)
     gene            complement(6214..6294)
                     /locus_tag="ACHA02_002561"
     tRNA            complement(6214..6294)
                     /locus_tag="ACHA02_002561"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6258..6260),aa:Tyr,seq:gta)
     gene            complement(6296..6371)
                     /locus_tag="ACHA02_002562"
     tRNA            complement(6296..6371)
                     /locus_tag="ACHA02_002562"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6336..6338),aa:Phe,seq:gaa)
     gene            complement(6407..6479)
                     /locus_tag="ACHA02_002563"
     tRNA            complement(6407..6479)
                     /locus_tag="ACHA02_002563"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6444..6446),aa:Asp,seq:gtc)
     gene            complement(6489..6561)
                     /locus_tag="ACHA02_002564"
     tRNA            complement(6489..6561)
                     /locus_tag="ACHA02_002564"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6526..6528),aa:Val,seq:tac)
     gene            complement(6577..6648)
                     /locus_tag="ACHA02_002565"
     tRNA            complement(6577..6648)
                     /locus_tag="ACHA02_002565"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6613..6615),aa:Glu,seq:ttc)
     gene            complement(6663..6752)
                     /locus_tag="ACHA02_002566"
     tRNA            complement(6663..6752)
                     /locus_tag="ACHA02_002566"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6716..6718),aa:Ser,seq:gga)
     gene            complement(6792..6865)
                     /locus_tag="ACHA02_002567"
     tRNA            complement(6792..6865)
                     /locus_tag="ACHA02_002567"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6829..6831),aa:Asn,seq:gtt)
BASE COUNT         2089 a         1509 c         1111 g         2163 t
ORIGIN      
        1 caatatattc tcttaaataa tcaaatttat ttttaagaat cctgaaaata agttttctaa
       61 atcataggag caacaaaaaa attcgataat taacaaaaat caaaatgatt aaatctagtg
      121 attgaaacat atcatttcaa aaaaattttg taaattcacc cataaatatc accctattta
      181 acttttttca tcgtacacta aaattcaaaa caactatctt taatctttac ttcttggttt
      241 tgttttaata aatttccttc aaaaatgcct ggagaaacta caatgatcac cgtatctttt
      301 tgtgcttgct ccaacttctt caaatctata atagctaaca gtgtcccttc ttttacctcc
      361 tggttacttt caacccgtat ctgaaacgga cttccttcga gttcaactgt atctaaaccc
      421 atatggatca gtatttttct gccagattca ctttcgatcg ttatggcatg tcgggtagga
      481 aatatattat cgattattcc tttaactggc gcatggatat atccatcatg attcgttata
      541 gcgtatccaa ctcctatcat ttttgaagaa aagacctcgt cttttactgc tgttaatgga
      601 ataatttgac cactagctac agcttttaga atgtttcttg ttttaaagaa atgcactttt
      661 tatcacctct actttaatgc tggccaatat tttttgttta ctttgatcat ttcatctagt
      721 attttttttg cgactctggc atttggtacg gtttgattca atgtaaatgc ttgtaaagct
      781 ttttgataag aaccttcaaa gtaagcatct actagtaact tctctactgc aacttgtgcc
      841 tccatcaatc ctttatgaaa atcactgatt ggttttctta gtgtaattgg ctccacacca
      901 gttgcaccaa catatgccgg tatttcaaca actgcatctg accgtagatt cggaatcgcc
      961 ccttgattag gaacgattag cataaagcga cgacgatcat cattcaacaa ggaaatagct
     1021 atatcgacga tatattgtcc atgctcccca aaattaaagt tcaatacttc ttccacaata
     1081 ccgcggcgta ttttatcagc catttctttg gtatttttct ctcgtccatc catgaccata
     1141 ttagcacgtg tatactcttt gtcagtatgc tcaacgacca tatcaggata taagtaatac
     1201 tctaagtaat tattcgggat attacttgga aaatttttcg tgatgactga catcatttga
     1261 aaggtttttt gccaagtttt atctcctata ttaaaatcgg cgacttgcat ttcttttgtt
     1321 attagttttt caataatctc tggcataatt tctcgttgca attctttatc ataaatcgat
     1381 gtataccagc caaagtggtt taaaccataa tacgtaacga tccaattctt acgatcataa
     1441 ccgtaattca ctgcaatcgt ttcttcaatt gaaatagtca tatcacaggc attaatcatt
     1501 tttacattcg gatatttacg acgaacagct tcagaaacga tcgactctgg atttgtataa
     1561 ttcaaaatcc atgctttggg ggcatagtct tgtatatgat cgacaatttc caataaacct
     1621 ttgattgagc gcatcccata agcaaatcct ccgagtccac acgtttcttg tcctactaat
     1681 ccatgtttaa gtggaatctt ttcgtccttt tctcgcatct ccaatcctcc aacacgaatt
     1741 tgtgagaaaa caaaatcgca tcctgtaaaa gcaagctttg gatcatccgt tgcacgaatg
     1801 cgaatatttg aaaaaccttt tcttttcagc ataaatttaa caatcaagta catatccata
     1861 tttctttctt catcaatatc atataaacgt atttcagata atggcaaccg tttttcattg
     1921 ttcaaaatgg cttgaacgat tcctggtgta tacgtactcc cgcctccagc tatagtgata
     1981 atctgttttt tcatcatcat cttttccttt ctattcttct aataatttat tgaattctgc
     2041 cataacataa gtcacttcta agccgatgat aatttgataa tttttttcag ttggtttaat
     2101 gatcccgctt atccctaatt gcttgattcg ctgttcctcg accatggtgg gatccactac
     2161 ctctacccgt aggcgtgtat tacaactata gacatccagg atattggctt ttcctccaaa
     2221 cgcttgtagc atttttgttg ccatatattc ataattttta cttgctaacc ctaactctgt
     2281 ttcttctaag gttacttcat tgccaaactc tgccttagct tcacgtccta atgtctgaat
     2341 gtttctctta ttgatcaaat aataaaaagt aacgtaatac aaggcaaaaa agaaaattcc
     2401 gattggaaga atcaaaatag cattggtagc gattcgaaaa ttgatgagat aatcaacaat
     2461 acatgctccg aatgtaaaac caattcgtat attgaataag taacagacaa tccctgctaa
     2521 accggcaaaa agtgcatgga tcacgtacaa cataggagaa agaaacatga aactaaattc
     2581 aacaggctca gtgatatttg caataaatga agttcctgca ccgcttccca tgattccttt
     2641 gaccatctct ttattttctt tataagctgc tttatagata gctagacata tagctggtat
     2701 accaaacatc attactacaa aaaaaactga taaaaacagt ccagcctgag gatctccgtt
     2761 gataaagcgt gtcatctctc ctctgaaaac ttccttgcta ggagatgtat agcttcctaa
     2821 atcgtaataa atatacgtat tcaacacatg gtgcagtcca aaggggatca gcaaacgatt
     2881 caaaaacata aatatcccca cacctaatgc tcccatatca acgagcagtt ttgcaaaatt
     2941 atcaatccca atttggatga gcggccagat aaagcccaaa aaaacagctg cgattgtttg
     3001 tagaatcatc accatagtca atgggaattt ttcgcccgca aaataagaaa aaactactgg
     3061 taatttttgt tctttaaagc gattatacgt ccatgccgct actaaaccgg aaataattcc
     3121 accaaaaatc cccatattca cttctgggtc cataatttct aagccttttt tcaaggtagc
     3181 aatcgctaaa aaaccagtaa gtgcaggtat tcctttgtcc ttatttttga caaaaccgat
     3241 tgcaactcct atagcaaata agatatccaa gtttccaaaa acggcatttc cagctgcacc
     3301 aattactggg atgttcagca tatcgtcagc tgataaacgc atgatcaatc ccgctaatgg
     3361 catcgctgca actggaagta gcatcgctct tccaagcttc tgcacatttg atttaacatt
     3421 catttttatt cctcctctat taaatatcct catatgttac taactcaatc aaaggatcat
     3481 ttttcacttt ttgagcaaat gctgaatgaa ttaaaaattc gacttggtgg gtacgaggat
     3541 acacaaaact tgaaccattt aacaattcct gatccaaata cgctgcgtga cacatgactt
     3601 cgatcgtttt atcttctctc aatatcatga gtaaacttgc caaatattct tccacactcg
     3661 tattgatttg tcgttttttt tcatcagttt ctcctacgac atcgaaacaa ggctcaaaat
     3721 aatcgacatg acgaacttca tcttttcgtt caaagttgct ccgaaccggc aaatcatatt
     3781 tttttgctaa tgtaacaact acctcctgat tttcatccaa agtatgagta tgatgatgac
     3841 tgtctaaatg agttggtatg atccccgcat gataaatttt ttgaatctga gcattccatt
     3901 cctgatacag ttgatctttg tcaatgttaa atggatgctg ataataagat aaggatctga
     3961 actctccgct ttcgataaga gtatcgacag ttttgaggag tggtttgcca cacgtcaatg
     4021 ttaaatggac tcctacacct agcctaggca tttcctttct tagtttcaca gcatgctcga
     4081 aacccggcat atttgccatc aatgtagttg atgtcaaaat tcctcgctga taagcatcca
     4141 taatcccata attcacacca tggctatagc caaaatcatc cgaattaaaa atcacttttc
     4201 ccatttcatt ctccttcttt tttctactaa aataggcgca accaaataat catagtccat
     4261 ttactatgtg tcatttggtt gcgcctatag atagttacgc ccttatttta atagattctt
     4321 gctagatgaa ttgccaaata gatgatttca ttttcttgaa tggcaagttg aaattgctgt
     4381 tcgatgatat ctttaatctt tagtgctgta caatacgctt tgctatgttt aaatgtttca
     4441 gtaaaaaaac caatattttc tactggcgtt tcttcctgat aaatcaagcg tttaaccagg
     4501 aatttgatat gaaccattaa gcgatgataa gtcagtgatt ctttatcata aacgtttgaa
     4561 taggattttt ctagatggga caaaatcgtg gtcgtcattt ctactatttt taagctcttt
     4621 aaactattag gctctcctag ctcagcatta atgatatgca tagtgatgaa tcctacttca
     4681 tttgacccta actcggtttg atatcgatga ttgattcgct cgaccacttc tactgctata
     4741 tcatattcat ttggataaag gaatctcatt tctggcaaca taattgctgt gacatctcta
     4801 tcttctcttt ttcgttgaat agcaaaataa atatgatctg tcaatgtaat atagatattt
     4861 gaatctaatg gagaggagat cctagctgtt atcagttcaa tcacttcttc cgcaaaatcc
     4921 ataaattcga ttggaatctg cgagactatc tcactgaatc gtttgatctt ttctttagaa
     4981 tctaatacaa ataatttgtc agcatcctca acatcaaatg gcttactcac tttagcagaa
     5041 aagccaatcc ctttccccat cgcaattttt tctattccat cgtcatcgat caatacagca
     5101 ttatgattca gctttttcat cacatacacc gttaattcac ctcctctata tgataaaaca
     5161 aaacaacttg cagactcctt catacatatt tttgtctcca aacaattttt aaagacaaaa
     5221 aacgacctaa gaaagaaccc tttcttaggt cgtgcctaat tgaatagtag caccctacat
     5281 aatttacaaa aatagtttaa cacataataa aagcgttatc aatatctttt ttactcgttt
     5341 tatgataata cttgtatcga attgatttgg aggtaattac ataaaaacaa aaaaaatata
     5401 gcaactctct ataagaaagt tgctatattg ataattatac cggcggccgg ggtcgaaccg
     5461 gcacgtcctc aacggacact ggattttgag tccagcgcgt ctgccaattc cgccacgccg
     5521 gcaaatttaa aaataataac tggggtagct ggattcgaac caacgcatga gggagtcaaa
     5581 gtcccttgcc ttaccgcttg gctatacccc aagaataaag ggcgaatgat gggaatcgaa
     5641 cccacgaatg ccggagccac aatccggtgc gttaaccact tcgccacatt cgccatcata
     5701 aaagatgtta tttaatttct caataaaaaa ctaagatgaa gacatcttag tcaataccgg
     5761 cggccggggt cgaaccggca cgccctcaac gggcactgga ttttgagtcc agcgcgtctg
     5821 ccaattccgc cacgccggca agttgattta ttagtactaa ggcggtaacc ggatttgaac
     5881 cggtgatgaa ggttttgcag acctctgcct taccacttgg ctataccgcc attatactat
     5941 tatcaatcta taaaataaag aataaattat agaaactggg gtagctggat tcgaaccaac
     6001 gcatgaggga gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaaaaggg
     6061 cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt taaccacttc
     6121 gccacattcg ccatggcagg ggcagcagga attgaaccca cactaacggt tttggagacc
     6181 gctgttctac ctttaaacta tgcccctata aaatggagga gagtggattc gaaccactga
     6241 accctaagga acggatttac agtccgtcgc gtttagccac ttcgctactc ctccatggtg
     6301 gcgcgggaca gaatcgaact gccgacacac ggagcttcaa tccgttgctc taccaactga
     6361 gctaccgcgc catattttaa aattattaat tgaataacaa gaacgacggt tccgacggga
     6421 atcgaacccg cgatctcctg cgtgacaggc aggcatgtta acccctacac cacggaacca
     6481 gttcattatg gaggttaacg ggatcgaacc gctgaccctc tgcttgtaag gcagatgctc
     6541 tcccagctga gctaaacctc caacattcaa ttaaaatgac ccgtacggga ttcgaacccg
     6601 tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc aacgggccag gtaaatttgc
     6661 tacggagagt aagggattcg aacccttgag acagtttggc accatctaca tgatttccaa
     6721 tcatgctcct tcggcctctc ggacaactct ccagagtgaa agaagctcaa tactctttct
     6781 caaaatgaga actccggcag taggactcga acctacgaca tcatgattaa cagtcatgcg
     6841 ctactaccaa ctgagctatg ccggaataac at
//
LOCUS       DAYYDS010000116         6696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000116 DAYYDS010000000
VERSION     DAYYDS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.121"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..529
                     /locus_tag="ACHA02_002568"
     CDS             <1..529
                     /locus_tag="ACHA02_002568"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8670846.1"
                     /translation="DHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDL
                     VKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFMATFEDV
                     EGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGI
                     SYDEYQKLEMGKKIV"
     gene            complement(983..2674)
                     /gene="argS"
                     /locus_tag="ACHA02_002569"
     CDS             complement(983..2674)
                     /gene="argS"
                     /locus_tag="ACHA02_002569"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8670847.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(3094..4041)
                     /gene="arcC"
                     /locus_tag="ACHA02_002570"
     CDS             complement(3094..4041)
                     /gene="arcC"
                     /locus_tag="ACHA02_002570"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8670848.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(4156..5175)
                     /gene="argF"
                     /locus_tag="ACHA02_002571"
     CDS             complement(4156..5175)
                     /gene="argF"
                     /locus_tag="ACHA02_002571"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8670849.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(5266..6495)
                     /gene="arcA"
                     /locus_tag="ACHA02_002572"
     CDS             complement(5266..6495)
                     /gene="arcA"
                     /locus_tag="ACHA02_002572"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8670850.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         1858 a         1394 c         1191 g         2253 t
ORIGIN      
        1 ggatcatatc caacagcttc tttccctccc ttctgtcaaa aaacaatgga tagctattta
       61 tggacaacta cctcaggaaa aagaggtgga ggaattgtat caacaatttg aacattatct
      121 gtttaaagat ttggtcaagc attctacctt gaaacccgac acattggaaa cgatcgaacg
      181 gttgagaaac gctgggatca atattggttc cacgaatgga tttaatgctc ctatgatgac
      241 aatcgtagca gaaaccgcaa aaaaagaaag gtattcacca gacttcatgg caacttttga
      301 agacgtcgaa gggtatggtc gaccgtttcc ttatatgatc cataaaaaca tgcagtactt
      361 caaaaacaag tcggttgatg aagtaatcaa agtcggagat acggtctcag atatccagga
      421 agggaaaaat gccggagtgt tgacagtagg tgtaattgaa ggtagttcgc taatggggat
      481 ttcttatgat gagtaccaaa aattagagat ggggaaaaag atcgtttaaa acagtctgtt
      541 gctcttagat tccaacaagc cggcgctgat atttgtgtaa acaatttaaa agagttaaca
      601 gaatacattt tgtacgctga catgaatcca gaatgacttt tatacaaaat aagaggttgt
      661 gaaaaagctg tcctgtatca agtaataaaa acagccaagc aaaaatagct cctcatattt
      721 ttcgtttggt taggcttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
      781 aaaaagggag ctgtgacacg acttttgtca cagtcccctt atttgcaaac aaacagtgtt
      841 taagagacaa cctctccact aaacggtgtt cattcagctg actctcggta tttgctcaaa
      901 aaacgataaa atttgaaaag ctattttctc ttttgtttct ctaatactcg gatcagaacg
      961 ctgatttcac attctctttt tcttacatct tatctggtgc atgaagtcct agcaaacgaa
     1021 ggtcttcttt caataataca gtcactgcat aaactagagc taagcgtgct tctttttgtt
     1081 catcatcagc taaaatcttc gtatgagcat agtatttgtt gaatgcttgt gctaatttga
     1141 ttgcatgttt cgcaatcact gacggttcgt atttttcccc agcagacaag accgtttcag
     1201 ggtatttttg aactaattta accacttccc agctggtatc gtcattcaat gcatagtctg
     1261 ctgtttctga agggacaaat cctgcttttt ccaacaggct gactgcacga gcatgtgtat
     1321 attgcacata aggtccagtt tctccttcaa atcgaacaac ttcttccagg ttaaagtcaa
     1381 atgtattcag tcgatcgttt ttcaaatcgt ggaaaatgac tgccccaacc ccaacttgtt
     1441 tagctacagc atctttgttt tccaaatcag ggtttttctc gctgatttgt tctttagcag
     1501 attggattgc ttcatttaat acttcttcta ataagacgat ctttccttta cgagttgata
     1561 attttttacc accttgcgtg atcaaaccaa atggaatatg gtgcatgtcg tcagaccagt
     1621 caaagcccat ttccttcaag acagctttca actgtttaaa atgatagctt tgttcattcc
     1681 ctactacata tagagattgt ttgaagtcgt aagtacgttt acggtacaat gcagcagcta
     1741 agtcacgtgt aatgtatagt gtcgcaccgt ctgacttctt aatcaacgct ggattcaggt
     1801 cataagcaga taggtcaacg atctctgcac ctttatcctc gttcagcaaa tgtttttctt
     1861 ctaaaagttt gacgatttca tccatcttgt cattgtagaa agcttcaccg ttcaacgaat
     1921 cgaaacgtac ttccagcaga tcatagattt tattgaattc tttcatcgat tcatcacgga
     1981 accattgcca taattgaata gcctctttat cgccttcttc cagacgtttg aaccaagcac
     2041 gagcttcttc atttaattct ggttctgttt cagctacttc atggaattgg acgtataaac
     2101 gaagcaattc attgattggt tctgctttaa cagcttcttc tgtaccccat tttttgtaag
     2161 cgacgatcag ttttccaaac tgtgttcccc agtcacctaa atggttgatg cggattggct
     2221 gataaccgat tttttccata atgaaaccga tcgaattacc gataactgtc gaacgcaaat
     2281 gtcccattga gattggtttg gcaatattag gagaagacat gtcgattggt actgttcctt
     2341 gattcccgat gttgctatcc ccataatgtt ctttttcttt cactactgtt tgcaaaactt
     2401 ttttgctgat aagttcttta ttcatgaaaa agttcaagta aggtccgaca acttcgattt
     2461 tttcaaagtt ggcagaatcg attttttctg ctaaatctgc agcgatttgc tgtggcgctt
     2521 ttcggtaaac ttttgctaat gaaaaggctg gaaatgcaac atctccgtgt tcagctgatt
     2581 ttggattttc tagtaattgc tcgacttgtt ccagtgttaa atcatctttg acagcgtcat
     2641 agacagcttt tgcgacaaga tctttattat tcatctttat tcctcctaaa gtgttccctt
     2701 gattgttaaa caaaaaaaat ctctagcgac ggcatactta gtatgccatc actagagacg
     2761 agatttcccg cggtaccact ccaattgtcc taaaaaagga ccagctccat ctgatatcgg
     2821 tagacgaccg tctgtttaaa acagaattct ccaagtgcgc ttcgttgtat cttcttcatt
     2881 cggcttccac cacttccgaa ttcgctagcc aagaatgata caagtactct cttgttcaac
     2941 aaatcttagt cttattaaac tttcttgaat tatatcagaa aaaaaaacga aaggctagtc
     3001 gtttcataaa aaaacaatcc agatggccta aaggagaaga aataccacct ggatagctat
     3061 aaatcatttc gtaaatacga aagacttaac aaattaatca gctacgataa tcgttccttc
     3121 ttcagaagct aaaagattct cgatattttc taaagaagtg atgattgcct tagcgtttgg
     3181 ttttgcttca acaaatgcaa tagctgcttc tactttcgga agcatgcttc ctggagcgaa
     3241 ttgcttttca tcaatgtatt gtttcatttc tgaaacagta acagtttcta atttcttttg
     3301 atctggtttt tggtaattga catacacatt gtctactcct gtcaatacga tcagcaaatc
     3361 cgcatcgatg atttcggcta atttttctga agcaaaatct ttatcgatta cggcttctcg
     3421 tccttctaat ccagtagctg tttctacaac aggaatccca ccgccaccaa cagagactgt
     3481 gataactcct tgatcgacca gcgtttcgat gacacgtgct tctttaatcg agatgggttt
     3541 aggggaagcg actacttttc tccatccacg tccagcatct tctttgaatg tcacagtgga
     3601 gtcagcattc atttgttctt tggcttcttc ttccgtgtag aatggaccga ctggtttact
     3661 tggatttttg aatgaaggat cattttcatc cacaataact tgagtgacta aagaaaccac
     3721 atctttatcg atcccttttt ccttcagtac ttcgcccatg gcgttttgta accagtaacc
     3781 gatagatcct tctgtcattg caacacatgt atctaaaggc attgccggtg ttttctcaga
     3841 atcagcagct tgctgctgaa tcaataggtt accgacttgc ggaccatttc cgtgggagat
     3901 gatcaattca tctccttgtt cgataaattt cactaaatac tttgctgttt ccattaacgc
     3961 ttcttgttgg gcttttgcac ttgcatccgt agacaagatg gcattgcccc ctaaagcgac
     4021 aactactttt cgtttggcca tttcattcgt cctttcttca gttctaccga attttctaga
     4081 taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga
     4141 tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta cctaatgttg ctgccatgat
     4201 cgctttgatt gagtgcatac gattttctgc ttggtcaaat tgacgagcat atttgctgcg
     4261 gaagacttca tctgttactt ccatttcagt gatgccaaag cgttctttca tttgttcacc
     4321 gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc aagaagatca aacgatctgt
     4381 gttgtgtgtt ttttctacca tttccatatt gatttggtaa ggtttcaaca atttgatacg
     4441 ttcttcgaat ttatcttctt cacccattga tacccataca tcagaataaa ggacgtctgc
     4501 tccgtctaca cctttatcaa catcgtcggt aatcatcaat tgtgcaccgg atttttcagc
     4561 aaagccttct gccattttga cgatttcttc ttcaggttgt aattcttttg gtgcaacgat
     4621 acgcatattc gctcctaaaa ttgcacctgt gactaataaa gagttagcca tattgtttcg
     4681 accatctccg cagtaagcaa cagtgatacc ttctactgtt ccaaagtttt cttggatagt
     4741 taaaaagtca gcgatcattt gtgttggatg ccattcatca gtcaagccat tccaaactgg
     4801 aacaccagag aattctgcta gttcctctac cattttttga ctgaatccac ggaattcgat
     4861 tccgtcaaac atacgtccta gtactttagc tgtatcttct gtggattctt ttttacctaa
     4921 ttggatatca tttgcaccta gatattcagg atgtgcacct agatcgatcg ctgcagtagt
     4981 aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa agcgcaatat tttttccttc
     5041 tagatagtga tgtgggatac cacgtttttt caagtctttc aagtgttctg aaaaatcgat
     5101 caaatattgt aattcttctt ttgtaaagtc tttttctgct aataagcttc ttccttggaa
     5161 tactgattct ttcatattaa acattctcct ttagattgtt tttatttaag attaaagagg
     5221 aaatcccgac ggcagctgac agtaagactg ccgtcaggaa acaaattact tcaagtcttc
     5281 acggacaagt ggttggctca tgcagcgtgg accaccacga cctcgtgaca attcacttga
     5341 attgatttct aacactttga tgccatagct acgaagcaat tcgttcgata cataatttcg
     5401 gttataagtg actacaacac ctggagcgat tgccaatgtg tttgatccat cattccattg
     5461 ttctcgaggt gcaacgatct catctccatt accagttggg atcagtacaa gatcatctaa
     5521 tccaagagca gctttcatcg tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc
     5581 accatctggt tcgattgtaa agacatccat cttaccgtct ttactttgaa ttgctggatg
     5641 gatagtgaat ttatcgtgat ctaccattgt aaatacggta tccaaatgca tcattgcacg
     5701 gttgttcgga attttgattg ccaacacttt ttcaaatcct gaattttttg cgaataggtg
     5761 gcgtgctaat ttttctaaag ctttcgcatt agttcgttga gaaatcccaa cagctactac
     5821 tttatcgctt aagatcaatt cgtctccgcc ttcgatatga tctggatttt cacgatccaa
     5881 ccatacttcg attcctttat ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa
     5941 catcgattca cgacgacgtg cttcaaaagt catcttattg actgtcatcc catttcccat
     6001 tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt gggtccatgt aaaatggata
     6061 gtcatcgtct gctgacagat catatagact gctagaacgt acttcgatat cttttgtacg
     6121 aaccccagcc atgattttat ctaccatttc ttgcgtttcc attgatagaa ggaactcatg
     6181 aagaccctct cttacagcat tcgatgcaat atgagattca tccaacattt tattcaagaa
     6241 ttgttctttg acattgcctg cgtcaatcgc ttctgccgct aatttttcca agtaaagagt
     6301 ctcgacgcct tcattttgca atgtcttcgc aaagttgtca tgttcttctt gagcaatcgg
     6361 aagatatgga atatcatcga acaacagacg atccataata tctggtgtca aattttccac
     6421 ttcttgtcca ggccgtttca gtaaaactgt ctttaatttc cctatttcag agaaaacgtg
     6481 aataggttta tccatgttgt gttgcctcct tctttttgat tacatgtata ggatatcctc
     6541 cttttgtaat cactttcata attttgtctc attcaaaatg agacattcct cacattaata
     6601 ttcgataatt aaataattaa acgtttttct atttttaagc gtaatttatt cattttttat
     6661 tctgtataca ctttcaaaaa aatatttatt cattga
//
LOCUS       DAYYDS010000117         6568 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000117 DAYYDS010000000
VERSION     DAYYDS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.122"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHA02_002573"
     CDS             127..579
                     /locus_tag="ACHA02_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670851.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHA02_002574"
     CDS             593..1282
                     /locus_tag="ACHA02_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670852.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHA02_002575"
     CDS             1407..3758
                     /locus_tag="ACHA02_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8670853.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACHA02_002576"
     CDS             3838..4233
                     /locus_tag="ACHA02_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670854.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACHA02_002577"
     CDS             4558..5175
                     /locus_tag="ACHA02_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8670855.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACHA02_002578"
     CDS             5216..5515
                     /locus_tag="ACHA02_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFM8670856.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACHA02_002579"
     CDS             5549..5716
                     /locus_tag="ACHA02_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8670857.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="ACHA02_002580"
     CDS             complement(5761..6366)
                     /locus_tag="ACHA02_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8670858.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2108 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattt
//
LOCUS       DAYYDS010000118         6564 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000118 DAYYDS010000000
VERSION     DAYYDS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.123"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..1122)
                     /locus_tag="ACHA02_002581"
     CDS             complement(7..1122)
                     /locus_tag="ACHA02_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670859.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="ACHA02_002582"
     CDS             complement(1167..1523)
                     /locus_tag="ACHA02_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670860.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="ACHA02_002583"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="ACHA02_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8670861.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="ACHA02_002584"
     CDS             complement(2991..3830)
                     /locus_tag="ACHA02_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8670862.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="ACHA02_002585"
     CDS             4015..4902
                     /locus_tag="ACHA02_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8670863.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="ACHA02_002586"
     CDS             complement(5486..6181)
                     /locus_tag="ACHA02_002586"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFM8670864.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="ACHA02_002587"
     CDS             complement(6165..6563)
                     /locus_tag="ACHA02_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8670865.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2065 a         1300 c          966 g         2233 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catc
//
LOCUS       DAYYDS010000119         6487 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000119 DAYYDS010000000
VERSION     DAYYDS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.124"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            413..619
                     /locus_tag="ACHA02_002588"
     CDS             413..619
                     /locus_tag="ACHA02_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8670866.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(821..1822)
                     /locus_tag="ACHA02_002589"
     CDS             complement(821..1822)
                     /locus_tag="ACHA02_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8670867.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1827..3740)
                     /locus_tag="ACHA02_002590"
     CDS             complement(1827..3740)
                     /locus_tag="ACHA02_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8670868.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3908..4414)
                     /locus_tag="ACHA02_002591"
     CDS             complement(3908..4414)
                     /locus_tag="ACHA02_002591"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8670869.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4574..5014
                     /locus_tag="ACHA02_002592"
     CDS             4574..5014
                     /locus_tag="ACHA02_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8670870.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5173..>6487
                     /locus_tag="ACHA02_002593"
     CDS             5173..>6487
                     /locus_tag="ACHA02_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8670871.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTT"
BASE COUNT         1847 a         1325 c         1050 g         2265 t
ORIGIN      
        1 tatttttgtt tcttattttc taaaaaatta aaaaaaattt tggtgaatta agtatttcct
       61 tgataacact ggcttttggc ctcattttag gttatcattt gtaattttat ttcacagttg
      121 atatgattgt tatgtaaaat aaaaaagttt aggatacatc gaatttttgg ggaaattctt
      181 ttgggggtat cctaaatctt ttctattata ttcccatatt ttctattatt ggtctagacg
      241 tatgatggta aactttgtac ctatctgatt gctgaatcat taattgctta tcattagcca
      301 tctttgtttc aagttcgcta gatgatcatt ttattcacat cctaacaaac ttgtcacatt
      361 tatagattct taatctattt atcatacaat tataaaaaag gaggtgctgc ttatgaaagc
      421 tatccatatt tctttgcttt ttttcgctag tgcattgatt ttatttgcta ttcatctttg
      481 ttcccagcct tatatcaata acaacggaat attagtggaa ccagcattct tttgcttacc
      541 actcggcttt ttgtcccttc ttgcctccgc aggcttcgga tttgttgctt tttttaaacg
      601 atcaaaacaa caaatctgac ttataaaatt tacgtaaaag agatcgtgac aataatcgtg
      661 tgatattccc aaatttcgaa taaaaggtgc cccttgccat ctctaactcc tcagtcacaa
      721 gttacaatat tcataaacca tgaaaagctt gtttattgaa attgctcctt gtgacttgga
      781 gctaagtgac agattacaac ctcttttgca tcataagact ttactccttg aaaaacatta
      841 ctaacttttg atatgtttct tgcgcttgtt tattgctgaa atcaaactga tattcatggt
      901 tcaaaacagc atttttctcc gcataatcta cttttgtgac tttcaccccc gctttctcaa
      961 atacagaagt tacttcttcc atttgtttcg taaacgtatt tgtatttcca tccgttaaga
     1021 atgtgttttg cgtccaacga tccgcatgtc tgaccaaggc taaacttttg accttctctg
     1081 attgttcaaa gtccttatta tcaaagtaag cccatccaca tttttcataa agaaagctag
     1141 agattttatc acttacttga gtaagtgcag aaggatccaa cagaccagac aaagaaataa
     1201 acttcgtaat ctgcttgttc tccaaagtcg gatcaagatg atcagctttt ttaatttctt
     1261 cattttgaag tgctaacaaa tattctccac taatttgtgc acctgccgaa tttccaccaa
     1321 tcgaaatatt cgaccaatct gcttgatctt ttatctcttt tttagctgct tgaatggctt
     1381 cgttcaattg gataactggt gtaggatgtt ttgcttccgg tgatcgttca taatctatat
     1441 ttacgatgat ttgctgcgtg tctgaacata gatattccaa ataatcacga caatcatttt
     1501 tatcccctcc tacatatgcg cctccatgca tccagaaaag aatcggtaat tttttctttt
     1561 ccactttcgg tgtgtagata tccatattat tatttttgta ctttgaagga taggatattt
     1621 ctttttttat atcgatttgt tcgatttttt gctggaaatc atctggtcgg gcatatactt
     1681 gttctttttc acttgtgtca aattgttttc taacaagcaa gctaatcggc tttggtgtga
     1741 tttgattaat caaaaataca gtgactaaaa taactacgat gataactggt actgcaatca
     1801 ctattcgctt gaaccatttc attcatttac cctctctttc ttcgccaaat ccatgtgata
     1861 atcgtgctaa cagataaaaa tccacctgaa taaatgagaa gcgaatgttt cttttctcca
     1921 gttttcggca acattctttt ttttgcatct gtcttttttt gtactttcac tgtcatctga
     1981 atcggttgcg tatcatcact tgattgatcc tctcgaggtt cattctttcc cttattgtct
     2041 gcattctctg tcggatcttg cttttttggt ggagctactg ggattttttt gtaatagaaa
     2101 ataatagttg gtatcgtctc attaaaaatc ccttcagtcg gtccttcaac atttgctaat
     2161 tcgtatcctt cgaatgtctt ttgctctgtc atataatttt ctccataagc tccgtaaagc
     2221 tggatttcgt cctggtcttt gagtagactt ttattgtttt catccacata ctttacgttt
     2281 atctgatcat ggaacactac tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg
     2341 tcaacttcct gtgcctttcc atgccctgct ccttctacta ataaaagatc tttataccct
     2401 cctgataact catataattt ttgcgcgtta gacacaggta ctacatcatc tgctgttcca
     2461 tggataatca atttaggcaa tgaatctgac attccaatcg taggtagagc agcttttgta
     2521 tccaaatgca tctcctgatc aaaatagtat tgtttcaaaa ggcccataaa taaatcttca
     2581 tgctcttgat ccccaatgat gtctaactga ttttttatgg catccggtag tgcgggggct
     2641 actgtctggc tgtacgtaaa acgtagttgt tcatcaattg atgcaaatcc gcaattttca
     2701 ataattcctt tgacattgac aggaatatct ttcgctaata cagacatcat cgtagctgct
     2761 cccatcgaac caccataaag taaaatcttt tgagatggcc gctctcgaac ttcttgattt
     2821 atccagtaaa ggacgtcatc tgactcatat tgtccatagg tcacataatc gcctccgcta
     2881 ttaccagtcg ctctggaatc aacaaataat aaattatacc cggccttata aaaatcattg
     2941 tattcttctg taacgatccc gttattccaa ttccctcgaa atccgccatg gatcatgact
     3001 gttttatctg aaccttgatc gacataatat gcatatacat ccatcatttt gccatcttgt
     3061 ttgtttagta cagtttgatg aagttgtttt gtatttggat agaagcctgt gttttctgta
     3121 ggcgttgtcc catcatctgt acctccaagc atcgacatca acacactgtt ttttgcaaaa
     3181 gcgatatcgt acaatagata agtagcaatc agatgtttga taatctcttt atctacacca
     3241 taatcattaa cagcaagctc agtgactaat aactctatta attttgactt gatgtcttct
     3301 gttggtccat taagtatttc tgtcatctgt ttaccgaagt aattcaaata ttctttacca
     3361 ttgaaatttt cacctttacc gatatcatca ttttctgtcc cattctcttt gtcacttact
     3421 ttagcagaag aggattcgtc gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg
     3481 ttatctgtgg tagcggtcgt ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt
     3541 tcagtatttg tttctgtttg tgaagtactc gatttcgttt catttccagc atcagtagtt
     3601 tctgtggttt ctacctcccg agattccgat tcggaagaaa tgctctctcc atatactgtc
     3661 cattgcggga cgcttgtact tacagcaaat aagatgacca acgctccctt taatacttgt
     3721 tttgttctca agctattcac tcctttttat gtttctagga gaattatatg taaacgcaaa
     3781 cattttttat acaaaaaata aatggtttat cccataaaaa tagaacagga ttattttctc
     3841 attttacgta ataaagcaga atggataaac cagtatccat tctgcttttt ttaaacgaag
     3901 aaacaactta ttgctccaaa aatgtcagaa atcgtttatc ccaaattttc tcgtaaaaag
     3961 cgattgtttc ctttgctttc ataaaaggat tatcataagt tgtcgttgct cctctacaca
     4021 tcataagctg gtatcctaat ccatgagcaa acttcacagt cgtatctata caatactctg
     4081 cttgtgcccc acataattcg atcgaatgac agtccaattc agttaataaa acactcaaat
     4141 tagtcttata aaatgaattc gcatgggttt ttctcacaaa ataactgtgc tcaggaatag
     4201 ataattccga caatagctcc catggttttg tttgtggcac taattgagca tcttcgtgtt
     4261 gaacgaaaat aactggtttc ccaatcgttt cgtatgtttt gattctttta ttcactaatg
     4321 ctgtcactgt ttctaattcg tgtatcggcg aagaatcaaa acatacccct ttttgtaaat
     4381 cgatcactac tattgcgtcg gctgtgtttg acatacttgt gccttctttc ttttttcttg
     4441 atttaatagt atttcttatc tagctagttc cattatacgt gacaacattt tcttatttta
     4501 cacctctaaa tggcactttt cgactatttc ttcatgttat tatttcataa atacttagct
     4561 attggaggaa tgtatgatga ttaaattgta tacaggttct ggctgctcgt cttcaagacg
     4621 cgcaaaacgt tggcttatcg tccatgatat accgtttatc gaacagaact taattcgtca
     4681 accactaacc aaaaatgaat ttatccactt actaaaattg acaaacaacg gcgtgaccga
     4741 aatgctctct actcattcta ttagttataa aaatcttgtt cacagaatag atgaactcag
     4801 tttgagtgaa ctatatattg agattcgtac atccccttct ttattgaaaa aaccgattat
     4861 ctacgaccaa tctgcaaaac ttttgactgg atttagcgaa gataaaatca ggatgttcct
     4921 ttctaaaact aaaagaaagc aagagctcaa cgaactcttg ctttctggtc atcttgtcga
     4981 ttttaaagaa aataatcttt ttgcaatgtc ttaattttgg cataaacaat ctgcttttct
     5041 ggatctataa tatctagtgt tggtgacgcc ttctgttgcc aatacttttt cttttttcta
     5101 ataaacgcaa acagacacct tgaaatcctt tagaataaag tcgacgaaaa ccattcaaag
     5161 gagtgtttca agatgtccta ttcagagttt accaaagagt tactagatat tctagaccta
     5221 aatcttacct ttcatgaaga tgcctttcgc aaagagcgca ttaatgacga aacatgtttt
     5281 gtttttgatg gcacattgac ctatcaacca gaggaatgct tccattgcca ctatcaaaat
     5341 aagcaaacga tcattaaatg gggctggaaa aaagtatcca tcttattgaa tgatgtcagt
     5401 aactacaaga ccattgtgcg gattaacaaa cagcgcttca aatgtaaaca ctgtggaaag
     5461 acttttttag cagaagattc agttagcgat cgtcgctgct ccattgcccg cagagtcaaa
     5521 caagccattc ttgagctatt aagtgagcct atttctatgt ctttgattgc acgtatgaag
     5581 cacatttctc cgaccactgt cattcgaata cttcgcagcc ttcggcctaa aaccgtttct
     5641 ttgaaccagc cgttaccaga agtggtgtgt ttcgatgaat tcaaatcggt gaaaaacgtc
     5701 tcgggagcca tgagctttgt catgatggac ggcaagaccc atcaactgat tgatattgtg
     5761 gaaaatcggc aactcaatcc tttacgcgac tatttcttgc gctaccctag aaaagtccgt
     5821 gaacaagtac gtctagtggt ttcagatttc tatacaccct atcgcacatt ggtgaaagag
     5881 tgttttccga atgcccgaat cgtcgccgat cgctttcata ttagccaaca catcggtcgg
     5941 gcctttacca accaccgaat tcaagtaatg aagaccttta aaaaagggga tcgtcggtcg
     6001 aagcacttaa agaaatactg gagactactt caaaagaatg cgtgggaatt gaatggtcag
     6061 catcgctact ggcgtccgtc atttcgagat catctgacag aagcggagat agtcgatcgg
     6121 ctattatatt atgatgactc cttaaagcga ggctacgaag tctaccaggt ttttctttcc
     6181 gctatcagaa gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga ctacaaggag
     6241 ttgccagagc actatcaacc agtgttcacg accttcaaga aatatcgaac agaaatcaaa
     6301 cgagcgttgc gagttcccta ttccaacggc cctatagaat gtttgaacaa ccatatcaaa
     6361 gtactaaaac gaattgccta tggctttaga aactttcaaa actaccgaga acgaatcttt
     6421 ttatatcgag gaaaatattt caagaaaact aagaacacta cccaactgac taaagccaga
     6481 acaacaa
//
LOCUS       DAYYDS010000120         6336 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000120 DAYYDS010000000
VERSION     DAYYDS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.125"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1348
                     /locus_tag="ACHA02_002594"
     CDS             <1..1348
                     /locus_tag="ACHA02_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8670872.1"
                     /translation="FLLVPLYTSVLSTTEYGTYDLTISTATLLFPILTLNIVDAVMRF
                     LMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLLNITKLWQDVYGLEKYIFLYYISYVW
                     NQFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLIGFFIANILSQIISA
                     TYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINSTADRYVVSFIVGVS
                     ANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYGNTFVMINLLMCITC
                     SSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGPILSAKKDSKSMMWS
                     AIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVGKDIFIKHYHIVVES
                     WGLLFVQAIIEIYFKNYVIEIIIMIILIIINISYIKQLCYRGGNIISMIKSREHKR"
     gene            1384..2100
                     /locus_tag="ACHA02_002595"
     CDS             1384..2100
                     /locus_tag="ACHA02_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8670873.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            2119..3702
                     /locus_tag="ACHA02_002596"
     CDS             2119..3702
                     /locus_tag="ACHA02_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFM8670874.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            3831..4415
                     /locus_tag="ACHA02_002597"
                     /pseudo
     CDS             3831..4415
                     /locus_tag="ACHA02_002597"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            4361..4879
                     /locus_tag="ACHA02_002598"
     CDS             4361..4879
                     /locus_tag="ACHA02_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670875.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            4902..5927
                     /locus_tag="ACHA02_002599"
     CDS             4902..5927
                     /locus_tag="ACHA02_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8670876.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            6176..>6336
                     /locus_tag="ACHA02_002600"
     CDS             6176..>6336
                     /locus_tag="ACHA02_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8670877.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         2292 a          727 c         1216 g         2101 t
ORIGIN      
        1 ttttttgtta gtccctttat atacaagtgt attatccaca acagaatatg gcacatatga
       61 tttgacaatt tcaacagcca cattattgtt tccaatttta accttaaaca ttgtagatgc
      121 tgtgatgcgc tttttaatgg ataaagataa aaataaaaaa aatatagcat ctatagcgat
      181 taaagttata tttttaagcg tgatagtctt tggatccgta atgtatttgt taaatattac
      241 aaaattatgg caagatgttt atggattaga aaaatatatt tttttgtatt atatatccta
      301 tgtatggaat caatttctaa tacaatttgc taaagggtta gaaaaagtta aagctatggg
      361 catagcaggt attatcagca caattgtaac aataattgcc aatattttct tcttgcttat
      421 tctgaaatgg ggattaattg gttttttcat tgcaaatatt ttatcacaga ttatttcagc
      481 tacttatctg tcagtattag tgagaatatg ggaatatatc gattttgagg ctaaagatcc
      541 tcagctaaag agagaaatgc taaattactc gattccatta attgcaactg ttgttggatg
      601 gtggattaat agtacagctg ataggtatgt ggtttccttt atagtaggtg tctcggcgaa
      661 tggcttattg tctgtggcat ataaaatacc tcaaataatt aatactttgc agggaatatt
      721 tatacaggcc tggcaaattt ctgcaattaa agaatatgga gataatgaga ctgcaaaatt
      781 ttatggaaat acatttgtta tgattaattt gttaatgtgc ataacctgtt caagtcttat
      841 catattaact agaccgtttt cacatattct ttatgcgaaa gacttttatg aagcgtggaa
      901 atatgttcca tttttattaa tagctaatgt tttaaattgt gcatcaggtc tgttaggacc
      961 tatcttatca gcaaaaaaag attctaaatc catgatgtgg tctgcaatca taggggcatt
     1021 agttaatgtg attatgagtg tagtccttgt ttataagata ggggttcaag gtgccacaat
     1081 agccactgtt atatgttcat atgtaatata tgttgttcgt aaacgagctg tgggaaaaga
     1141 tatttttata aagcattatc atattgttgt ggaatcttgg ggattattgt ttgttcaggc
     1201 aatcatagaa atatatttta aaaattatgt aatagaaatt ataattatga ttattttaat
     1261 tattataaat atcagttata ttaagcagtt gtgttataga ggaggaaaca ttatttcaat
     1321 gattaagagt cgagagcaca aacggtagac gatgttattt ttataaaaag atataattta
     1381 ttaatggtcg ataattatga aaggaaaaat attcttaaaa aaaagagtat aggatgtcgt
     1441 atgaaaatag tcgctatagt accaatgaag ttaaataaca aaagattgcc acaaaaaaac
     1501 acgaaagctt ttaccaatgg aaaaccattg tgttattata tactttcaac acttttaaaa
     1561 gtaaaacaga ttgataaagt gtatgtctat tgtagtaacc cagatattaa agaatttata
     1621 ccagaaggtg taacattttt aaagagatct agttccttag atcaggatac aacgagtatt
     1681 acacaggtat tagccggttt cacaaaagaa gtagaggctg atgtatatgt aatgacacac
     1741 acaacagcac catttattag cgaaaaatct attgaaaaag gactagatgc cgtgctgaca
     1801 ggagaatatg attctgcatt tgctgcaaaa aaattgcaag atttcttatg gaaagacggt
     1861 gaaccattta attatgaatt ggacaatatt cctagaacac aggatttgcc gcctttatac
     1921 gaagaaacta gtggatttta tatttataat cgcaatgtga tgaccgagtt gcatagaaga
     1981 attgggaaaa aaccttgcat agttgaagta ggtgagattg agtcagttga tattgatgaa
     2041 gccgaggatt ttatgattgc tgatgcgatt tataatcatc ttattttaca aggtagttaa
     2101 gacaggggga aggttaggat gaacaatgta cagattttag attgcacact tagagatggg
     2161 ggctattgta acaattggga atttggattt gataatacaa aaaaaattat ttctggactt
     2221 atagaatcag gaattgagat tatagaatgt gggtttctca caaataaaac agtatacaat
     2281 aaaaatgtta caaaatatac gaccttagaa gaattagcac agataattcc tgaaaaaaga
     2341 gaaggaaaat tatttgtagc aatgatgaac tatggcgaat ataatataga ggatcttcca
     2401 gattatgatg gttcatctat tgatggaatt aggattgcgt ttcataaaaa aaattgcaat
     2461 gaagcgttga aattatgtcg tgagataaag aaaaaaggct acttagtttt tgttcaagct
     2521 atggtatctc tcaattatac agatgaagag tttttagcag taataaaaaa tgtgaatcag
     2581 tttaaaccat acgcttttta tattgttgat agttttggaa tgatgaaagg aaaagattta
     2641 actcgtcttt tttacatgat tgagcataat ctagaaaaag atatacagat aggttttcac
     2701 tcacataata acatgcagct tgcatattct aatgcacaaa aacttataac cgtacaaaca
     2761 aatcgaagcc tgattattga ttcttcggtg tacggaatgg gaagaggagc aggtaatctt
     2821 aacacagaac tatttgtaga atatttgaat gataatgcag gaaaaaaata tcaattaaaa
     2881 ccattattat atataattga tgaaatacta aatagtttct atcagaagag ttattgggga
     2941 tattcattac caaactatat ttcagcagtt cataatgctc atcctaatta tgcaagctat
     3001 ctgtcatata agaatacttt aacaattgaa gcaatgaatg aaatattcga aatgatggat
     3061 gaagaaaaaa aggtatctta tgataaacaa tatatagaag atttatacta caaatatatg
     3121 gaaactggga aaatacagga ggaatatatg gaagaactga gaaaatttct ctcagacaaa
     3181 aaaattctac tgattgcacc aggaaaaagt tctgtagatg aggtagaaag aataaaagag
     3241 tttgttacca aagaagaagt cgtaataatt agtgtaaatt ttgaatattc tcatatagat
     3301 atagattacg tatttttaag taatttaaga cgttttcatg aattaccgaa agaaagcaga
     3361 actaaatgta ttgttacatc aaatatacaa gcatcggatg tatatttacg gacgagttat
     3421 aaaaagttgt tatctgaaag ggaagttgta agagacaatg caggattaat ggcaattaga
     3481 tttttagccg atatgaatgt ggatgaaata ttcttagctg gatttgatgg atattcacat
     3541 aatgaagatg agaattatgg tgagcaatcc atggaaatta taacaaaatt tgcattatta
     3601 gatgcaatga acgtgcaaat gagagatgaa ttgtcagaat tagctaaaaa agtaaaggtg
     3661 acatttttaa cggatccacg ttgggttcga attgataagt agtagtaata atgtttttaa
     3721 aattttgata aagagatagt gagagaataa aaatgggtaa agtcttaaat aaaaaagaag
     3781 aaaaatattt aagggacgat ggggcgagat gaaactctaa ttttgaactt ttaagaatca
     3841 tttctatatt gtgtattgtt gcttaccatg ctaatttgcg tttaaattat aatgcagacg
     3901 gaattatagt acatggttca ttaactgcta accagatttt cttgaattat attggtattt
     3961 ggggtcagct aggagttaat tgttttatat ttatttcggc atatttttta agtgagaaaa
     4021 gaaacattca ctttgaaaat gttttgggaa ttgtattaga atgtattttt tatagtgtat
     4081 tagtttatgg aattctttgt gcatgtggaa tagaaacatt ttcattgaac agctttgtaa
     4141 aagaaatatt atcatttgct ctaggtagat attggtttat tacatcctat ttagcattgt
     4201 atattctaag tccgtattta aatatcatgc ttaacaatct ggaaaaactt cagttgcaaa
     4261 aaataagtat gttattgttt ataggtgtat gctgttatcg tatgttattt aaaacatctt
     4321 tgtttgggga ttgtgctgtt ttaattgcac tatatgtcat atgttataca ctgaaaaaaa
     4381 cgataataat atttttgaga gacacagaat cataggaaca attgtatgta taacagttat
     4441 accacttctc atatccatag gaatactttt caaatttata ccaaatgcaa tggttaacca
     4501 gatggttcat attggttctc cattgatggt tatagcaagt atatttttat tttatgtatt
     4561 taaaaatttt tcattttaca gtaagtatat aaataaactt gcgagaacca gtttaggggt
     4621 atatatttta catgaaaatc attatttcat tgatggtatg aagtctagag gcttaatatg
     4681 ggatgggata tttggattag gcaaaaggtt tttagatagg gatttttgga tttggtttat
     4741 aattgcagtt gtagttgtgt gcataatttg catggctatc gatgttgtaa gagaaaaatt
     4801 tgaaagacag atttttaaaa ttaagagtgt aaagaacctg tgtaaactta tgaatcatgt
     4861 tataaatata tacccataat taataaatga ggagaaaaga agtgaaagtt atatcattcg
     4921 gagaattatt attgagactt gcagcacccg gttattcaaa gttatttcaa agagatagtt
     4981 tggagactac attctgcgga ggggaagcta atgtagcggt atccttagca atatttggat
     5041 atcaatcaga atttataaca aagcttccgg attcagatgt tggatatgcg gcagctaatg
     5101 ctttgcgtta ttttggagta aatgttgata atattatatt tggagaggga cgcatgggac
     5161 tgtattattt agaaaaagga gcagctcaga gaccatctaa agtaatttat gacagaaaat
     5221 tttctgcaat ttcgttagct gatagtgatg aatttaagtg gaaggagatc ctaaacgatg
     5281 caaattggtt tcattggaca gggataaatc cagcattgtc tgagaatctc tttgagacgt
     5341 ccttagatgc atgtcaggct gccaaaaaaa gaggtataac aatttcttgt gatttaaatt
     5401 atcgatccaa gctttggaca tcagaggaag caaaagaaaa gatggaacag atactaccat
     5461 tggttgatat ttgtatttgt aatgaagaag atgcagaaaa agtgctagga attaaagctg
     5521 agcatacaaa tattaatagt ggagaattaa ataaagatgg ctatgtgcag gtggctaggg
     5581 agattcacaa gaagtttgga tgtaggtatg tagctacaac tctgcgtaga agttattcgg
     5641 caagtagaaa tggttggagt gcaatgttat acgaagcaga aaaagataaa gcgtattttt
     5701 ccacagaata caacatacaa attgttgatc gcgttggtgg tggagacagc ttttctgctg
     5761 gtataatata tgcgttatgt gaaaatatgt ccggacaaaa ggctattgaa tttgcgactg
     5821 catctagttg tttaaaacat actatagaag gtgattttaa tcgaactaca attgaggatg
     5881 tatataattt aatgaatagc ggtggaaatg gtcgcgtgtt gcgttagtca acatataaag
     5941 ataaatcaca gtaattttta tggtgattat ctgtcttagt agtgattaga ataacgtgaa
     6001 aataaattgt tgagcgttta tactgttttg aaaaataaat ttaacatatt gaaaaataca
     6061 aatatatacg tatattataa agcgccgatt gtaaaattaa gctagacaac taaaacagtc
     6121 atttgtgcta cactcaaaat agttccgacc aaagaattat aaggagtgag tgcaaataac
     6181 ctatacctat cttacaacgg atgaacttgt aataatagag tcttatttga aaattaacca
     6241 atctgttgct aaaactgcgc attgcttgaa tcgttcaagt caaacgatcc ataaagtata
     6301 tctgttcttc aagcaaagaa aatcagcctt agaata
//
LOCUS       DAYYDS010000121         6303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000121 DAYYDS010000000
VERSION     DAYYDS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.126"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACHA02_002601"
     CDS             334..714
                     /locus_tag="ACHA02_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8670878.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACHA02_002602"
     CDS             696..1610
                     /locus_tag="ACHA02_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8670879.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACHA02_002603"
     CDS             1855..2544
                     /locus_tag="ACHA02_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670880.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACHA02_002604"
     CDS             complement(2665..2751)
                     /locus_tag="ACHA02_002604"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8670881.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACHA02_002605"
     CDS             3427..4377
                     /locus_tag="ACHA02_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8670882.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACHA02_002606"
     CDS             4398..4931
                     /locus_tag="ACHA02_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8670883.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACHA02_002607"
     CDS             4947..5117
                     /locus_tag="ACHA02_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670884.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACHA02_002608"
     CDS             5567..6100
                     /locus_tag="ACHA02_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670885.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACHA02_002609"
     CDS             6112..>6303
                     /locus_tag="ACHA02_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670886.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAYYDS010000122         6287 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000122 DAYYDS010000000
VERSION     DAYYDS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.127"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACHA02_002610"
     CDS             190..690
                     /locus_tag="ACHA02_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8670887.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACHA02_002611"
     CDS             787..2634
                     /locus_tag="ACHA02_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8670888.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ACHA02_002612"
     CDS             2637..3533
                     /locus_tag="ACHA02_002612"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8670889.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ACHA02_002613"
     CDS             3583..3972
                     /locus_tag="ACHA02_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8670890.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="ACHA02_002614"
     CDS             3959..>6287
                     /locus_tag="ACHA02_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8670891.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAYYDS010000123         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000123 DAYYDS010000000
VERSION     DAYYDS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.128"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHA02_002615"
     CDS             725..811
                     /locus_tag="ACHA02_002615"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8670892.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHA02_002616"
     CDS             complement(932..1621)
                     /locus_tag="ACHA02_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670893.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACHA02_002617"
     CDS             complement(1628..1906)
                     /locus_tag="ACHA02_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670894.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACHA02_002618"
     CDS             complement(1866..2780)
                     /locus_tag="ACHA02_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8670895.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHA02_002619"
     CDS             complement(2762..3142)
                     /locus_tag="ACHA02_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8670896.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHA02_002620"
     CDS             complement(3592..3879)
                     /locus_tag="ACHA02_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8670897.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHA02_002621"
     CDS             complement(3899..4123)
                     /locus_tag="ACHA02_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8670898.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHA02_002622"
     CDS             complement(4578..4748)
                     /locus_tag="ACHA02_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670899.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHA02_002623"
     CDS             complement(4763..5296)
                     /locus_tag="ACHA02_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8670900.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHA02_002624"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHA02_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8670901.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYDS010000124         6143 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000124 DAYYDS010000000
VERSION     DAYYDS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.129"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1387)
                     /locus_tag="ACHA02_002625"
     CDS             complement(<1..1387)
                     /locus_tag="ACHA02_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670902.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQII"
     gene            complement(1685..2620)
                     /gene="murB"
                     /locus_tag="ACHA02_002626"
     CDS             complement(1685..2620)
                     /gene="murB"
                     /locus_tag="ACHA02_002626"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8670903.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2693..3613)
                     /locus_tag="ACHA02_002627"
     CDS             complement(2693..3613)
                     /locus_tag="ACHA02_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8670904.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3726..4478
                     /locus_tag="ACHA02_002628"
     CDS             3726..4478
                     /locus_tag="ACHA02_002628"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8670905.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5342..5875
                     /locus_tag="ACHA02_002629"
     CDS             5342..5875
                     /locus_tag="ACHA02_002629"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8670906.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1853 a         1176 c         1025 g         2089 t
ORIGIN      
        1 aaattatctg ttctttatta aatttttcaa gtaaacaatc agataaataa atattcgtac
       61 gagaagtgat tcgactctca atcacaatat tgtaattttt aaatcctttc acttgagaaa
      121 taatgtaatc ggtataagca gatccttgcg aaaaatcaat acagacatga atcggtgctt
      181 caaaatatga taccggacaa ataccgacca ataaaaagag ataatcataa aataagcgaa
      241 tcatgaaacg ttcatctttt cccaagaaat agcgtttatt ctcttgtatg accttttgga
      301 ttgctgtact gaatattgga tacgtctcga agaaaaactg tagctgcttt ttagacgtaa
      361 agctagtcat ctcaaagtcg aatatccaat tttttcgatt gacatgcatc atacccatac
      421 gaagttcttt tcgtatatat agtctttctt cttcagagat ttcaatcgaa aatggtaatt
      481 gatcaatcaa ttccactgac attttttgac taatcatgtg gatatcatga aaatgagctt
      541 ccgcaagtcc aaatgcgcca tctaattcaa attcttcttc cgtgatcaaa aacaacatga
      601 tataagaaat ctcatcaatg atctgctctt tagggatatg aagaaaagaa atatgtgata
      661 aatccttgat gatcttcatt ccttctgagc tcttaaagta ttcatctgaa aagaaacgtt
      721 ttccgattgg ttgattccca taggccctca acaaactaat agtggttagc aaattgaatt
      781 tgattcgttt tgttgcagac agttttaaat tgaacagata ttcaaaatat tgcacgatac
      841 tctttgcttc attcatgacg ctttctctaa atggcataga aaaaccatta aagaccccga
      901 aatatacccc aaataatacg ttccgtatgt ttatttcttc cccaactaat atgtttttct
      961 ttaactttat ttgatgctgt tcaagaaaag agactaattc tcttcttttt acataggctc
     1021 ttgatatgct taaaaaatga ttctctgcaa atttttcgat cgttactttt gaatccacga
     1081 tctccgttaa cagtcgagca atttttgatc gttgaaagta gattcttctt aaatttttga
     1141 tatccgtcaa attcagacgt tcgacatgga tctctgcttc ttctgaaatt tggatctctg
     1201 gctttgtttt gaattcagta aattctttat ttaattctat gatataattt cttgttttaa
     1261 atttagacaa acctaatgtt tcatataact taggcagcac tacataattc tttttctcaa
     1321 attctaaata tctagctagc agcagttttt ctctatcttc ctcttctata aggtaatcca
     1381 acttcatatc ctacacctca aaaaatgcct cataccaatt acttgatatg cagatgaaag
     1441 ttcatctttt cacctatact gattttatga tggattatgc ttgttttttc atcagttatt
     1501 ctcatttttt atcttttttt tgagaatatt gcaaaaaatg gaaaaattta tgaggaaata
     1561 ttctatattt tttgttttat tgtgttaaaa tgtacaagat ttgcgccatt tgcgtaaaca
     1621 accacaaaaa agagcaggac ggccaccgtc ctgctctttt acttatttta agttccatta
     1681 cttattaatt ggctaaactt ctgcttgttt ctgatattgc ttgtttttct ccaatgatcc
     1741 gcacttctgt ttctaaagac acatcaaatt tttctttgat tacttcttgg atgtgcgcaa
     1801 tcaattcaat ataatctgtc gctgtcgcat gatcgatatt gacgatgaat ccagcatgtt
     1861 tttcagagac ttgtgcaccg ccccatttca ggccttgcaa tcctgcttgt tggatcagct
     1921 gacctgtata gtgtcctact gggcgcttaa agacacttcc gcatgaaggg tattctaatg
     1981 gctgttttga ttgacgcaat tcggtcaact catccattct tgctttgatt gcttcataat
     2041 cccctgtttg caaactgaat cgagcttcca atacgatagc acgtaattct tggactttac
     2101 tgtgacgata agaaaatacc atttcgtctt ttgtcaaagt tttcaatgtt ccatcttcaa
     2161 gcaaaagatc cacttcagca aaaacatctt tgatctctcc gccatacgca cctgcattca
     2221 tataaaccgc tccaccgaca cttcccggga tgccgcaagc aaattcaaat ccagtcaatg
     2281 attctgccaa agctgcataa gtcgtatcaa tcagtgaggc acctgcttcc gcaatgacca
     2341 tcgttccttt gacatgaatc tgtttcattt cagtcagcat gatgactaca ccgcgaatcc
     2401 cgccatcttg aacgatcaga ttgcttgcat ttcctaatac catccaaggt atatcatgga
     2461 ttcggcaata atcgatgatt tgctttactt cttcttgttt tttcgggaat gcaaggacat
     2521 cagctggtcc acctgttttc gtaaacgtat agttcatcaa tggttcatca aacaatatag
     2581 taatcgtggc taagctttca actatttctt ctttattcac tgaggatttt cctttctttt
     2641 ctcaaacgtc ctcattattc tatcacgctt aatacaatct tgccagtctt gttcaaagtt
     2701 gatgacggta gtgatcttta agcacttcct cacataattc atgacttctt acaacttctt
     2761 tttgtttaag caattctgtt tccgatttac ctaaacggat ctgagaaaag aaggcctcta
     2821 ccattggctc aaacccgcgt ttgactagcg tgtttgtcca gtcgccaaat tctttcactt
     2881 tttttccatc agcctcttga atgacaagct gggtcagatc gtttacttca taagtaccat
     2941 ttttacttgt tacttgatat ttttctatat tagcgccact tgctaaatcc atcgttaaaa
     3001 tagccgtttg attttgtgtt tctaattgta aaatggcata ttccaaaaat tcctttgttt
     3061 ctcttatctg gtattttgtt ctcaaaatgg gttcatctaa taaatacaca gctgtatcaa
     3121 taagatgtaa aaacaaatca taaaggacga actctgtcgt ttcttttgcc gctattcgat
     3181 ttttttgcag gtggatcaac cgcttatctg ggatacgttt caattcttct accataggag
     3241 caaagcgccg gttgaaaccg atcatcaaaa ttatttggtt cttatcagct aattcttgca
     3301 attcacgaac ttcatttaga tggacactca gtggtttgtc aatataaaca tggataccat
     3361 tttctagaca ttttttggct aattcataat gtacttttgt tgctgcgtgg atcatacatg
     3421 cttcgatttt ttcttctaaa agctcatcga tcgtttcata tagatgttgg aacccgtact
     3481 ctttttgaat tctttctttt gtttctttat ttcttgttgc aaaataaaaa tcaagtgttc
     3541 cttgtttttt cgcataagtt ggtaaatatg ctttttctgc gatattccct acaccgatta
     3601 caccaatctt catctacttt cctcctggat catttagtta ctttcatctt acaccatttt
     3661 ttactaaaat gaggtacaat agtatcgcaa aggtaaggaa attaattcga aggagtgtgc
     3721 ttttattgaa atttatctct tggaacgtaa acgggcttcg tgccatcgta aataaaaatt
     3781 ttttagaagt ttttcatgag ttagacgccg attttttctg tctgcaggaa acgaaactcc
     3841 aagcaggaca aattgattta gatcttccag gttattatca atattggaat tacgcggaac
     3901 gaaaaggcta ttccggcaca gcgatttttg ctaaaaaacc agcattgaat gccacttacg
     3961 gaatgggtat cgatatccat gatacagaag gccgtctgat tactttggaa tattccgatt
     4021 tctttcttgt aacctgttac acgccaaatt ctcaaagcga attaaaacgt ttagattacc
     4081 gattagaatg ggaagaagcc ttttataatt atttggaaaa tctaaaaaaa caaaaaccag
     4141 tcatcgtgtg tggggatcta aatgtcgctc atcaaaagat tgatttgaaa aactggaaaa
     4201 cgaatcaaaa aaatgctgga ttcactccag aagaaagagc ggctctttct cgactgttgg
     4261 ataacgggtt tatcgataca ttccgttatt tttatccaac ccaggaaggt gtctactctt
     4321 ggtggaacta ccgattcaat tcaaggaaaa acaatgctgg ttggcggatc gattactttt
     4381 tgacgagcaa agatttagag ccaggactag ctgatgcaaa gatccatacg gacattatgg
     4441 gcagtgatca ttgtccagta gaattagatt tgaaataaag aggttgtgaa aaagtcgttt
     4501 agtttcgagt attaagaaaa attttgtgaa aatggctttc cacattttgc ataaatttgg
     4561 cataataccg agaaactggc ttttgaacac tgtttatcga gagattgtga aaaagctgtc
     4621 ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatattttt cgtttggttg
     4681 ggcttattac cgcagataca agcttttgaa caccgtttat taagaggttg tgaaaaagct
     4741 gcactgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt tttcgtttgg
     4801 ttgggcttat taccgcagat gcaagctttt gaacaccgtt tattaagagg ttgtgaaaaa
     4861 gctgcactgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat atttttcgtt
     4921 tggttggact tattaccgca gatgcaagct tttgaacacc gtttattgag aagttgtgaa
     4981 atcagcgtcc tgacctgagt attagagaaa caaatggcaa aatagttcct catattttgc
     5041 tattttttga gctaataccg agggtcagct gattgaacac cgtttagtgg agaagttgtg
     5101 aaaaagtcgc ctactttcga gtcgtaagaa atttttttga aaatggttcc tcacaacttt
     5161 gcataaattt gacttaatac cgagaagtca tgatccccat ctaactccaa atcacaagga
     5221 atgagttcaa aaactagctt ttctcaacaa atattacata aaaaattcta tgttttttgt
     5281 taaatttatt tgcttttttc aaaagtatgt tacagtaatt tcccaaggag gttttacaga
     5341 aatgaatttt gttgccatgg attttgaaac tgccaatcat cagccttata gtgcttgttc
     5401 tcttgcgcta gtcatggtaa aaaatagcca gatcgttgat gaattctata cattgataca
     5461 gcctgagact ccttttttct ggcgaaatgt ccaaattcac ggaatccatc aagaagacgt
     5521 acgcaatgcg ccgaaattcc cagaagtctg gcaaactatc caaccatatt ttcaaaaaaa
     5581 tcgtttagtc gttgcacata atgctgcttt tgatacaaag gtcttagcgg gatgcttaga
     5641 ttattatcat cttgaacagc ctaattatct ttctttatgt acagtgaaaa gtagccgtcg
     5701 cctgtttcct gaatttccaa atcatcgatt gaacactgtg tgcgaaaatt tgaacatcca
     5761 attagatcat caccatgatg cattggaaga tagtcgtgca tgtgcggaaa tcctgcttta
     5821 tcaagaaaaa aaattcggga cagatccatt gaaaaaactt gtgactataa agtaaaagaa
     5881 tacttatgaa acacacaaag aggtcgtgag cctgccgttt cactccgagt cacaaggagc
     5941 aatttcaaaa aatagcttct catgtttatt aaatattgtg actggtgacc gaggagctgg
     6001 caggagaaca ccgtttattg gagaggttgt gaaaaagctg ttctgcatca agtaataaga
     6061 acagccaaac aaaaatagct tctcatattt ttcatttggt tggacttatt accgcagatg
     6121 caagcttttg gacaccgttt acc
//
LOCUS       DAYYDS010000125         6001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000125 DAYYDS010000000
VERSION     DAYYDS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.130"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACHA02_002630"
     CDS             <1..395
                     /locus_tag="ACHA02_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8670907.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ACHA02_002631"
     CDS             398..1228
                     /locus_tag="ACHA02_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM8670908.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ACHA02_002632"
     CDS             1244..2095
                     /locus_tag="ACHA02_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8670909.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ACHA02_002633"
     CDS             2092..2451
                     /locus_tag="ACHA02_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8670910.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ACHA02_002634"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ACHA02_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ACHA02_002635"
     CDS             2624..2929
                     /locus_tag="ACHA02_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670911.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ACHA02_002636"
     CDS             2929..3240
                     /locus_tag="ACHA02_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8670912.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ACHA02_002637"
     CDS             3237..3410
                     /locus_tag="ACHA02_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670913.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ACHA02_002638"
     CDS             3407..3823
                     /locus_tag="ACHA02_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8670914.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ACHA02_002639"
     CDS             3823..4491
                     /locus_tag="ACHA02_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM8670915.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ACHA02_002640"
     CDS             4488..4865
                     /locus_tag="ACHA02_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670916.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ACHA02_002641"
     CDS             4862..5161
                     /locus_tag="ACHA02_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670917.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ACHA02_002642"
     CDS             5151..5309
                     /locus_tag="ACHA02_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670918.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ACHA02_002643"
     CDS             5403..5819
                     /locus_tag="ACHA02_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8670919.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2230 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 g
//
LOCUS       DAYYDS010000126         5809 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000126 DAYYDS010000000
VERSION     DAYYDS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.131"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            450..1604
                     /locus_tag="ACHA02_002644"
     CDS             450..1604
                     /locus_tag="ACHA02_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM8670920.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1577..2020
                     /locus_tag="ACHA02_002645"
     CDS             1577..2020
                     /locus_tag="ACHA02_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670921.1"
                     /translation="MGQYHYKLVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="ACHA02_002646"
     CDS             3143..4075
                     /locus_tag="ACHA02_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8670922.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="ACHA02_002647"
     CDS             4092..4607
                     /locus_tag="ACHA02_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8670923.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="ACHA02_002648"
     CDS             4903..5184
                     /locus_tag="ACHA02_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670924.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="ACHA02_002649"
     CDS             5186..5602
                     /locus_tag="ACHA02_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670925.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DAYYDS010000127         5790 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000127 DAYYDS010000000
VERSION     DAYYDS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.132"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHA02_002650"
     CDS             complement(99..1229)
                     /locus_tag="ACHA02_002650"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM8670926.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ACHA02_002651"
     CDS             complement(1877..3079)
                     /locus_tag="ACHA02_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8670927.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ACHA02_002652"
     CDS             complement(3139..3342)
                     /locus_tag="ACHA02_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8670928.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4551)
                     /locus_tag="ACHA02_002653"
     CDS             complement(3484..4551)
                     /locus_tag="ACHA02_002653"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8670929.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4609..4716)
                     /locus_tag="ACHA02_002654"
     CDS             complement(4609..4716)
                     /locus_tag="ACHA02_002654"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670930.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4879..5691
                     /locus_tag="ACHA02_002655"
     CDS             4879..5691
                     /locus_tag="ACHA02_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670931.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAYYDS010000128         5765 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000128 DAYYDS010000000
VERSION     DAYYDS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.133"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..392
                     /locus_tag="ACHA02_002656"
     CDS             162..392
                     /locus_tag="ACHA02_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8670932.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="ACHA02_002657"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="ACHA02_002657"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8670933.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="ACHA02_002658"
     CDS             1803..1979
                     /locus_tag="ACHA02_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8670934.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="ACHA02_002659"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="ACHA02_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8670935.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="ACHA02_002660"
     CDS             2474..2941
                     /locus_tag="ACHA02_002660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8670936.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="ACHA02_002661"
     CDS             3026..3910
                     /locus_tag="ACHA02_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8670937.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="ACHA02_002662"
     CDS             4024..4260
                     /locus_tag="ACHA02_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8670938.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="ACHA02_002663"
     tRNA            4348..4428
                     /locus_tag="ACHA02_002663"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="ACHA02_002664"
     tRNA            4435..4506
                     /locus_tag="ACHA02_002664"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="ACHA02_002665"
     CDS             4631..4801
                     /locus_tag="ACHA02_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670939.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="ACHA02_002666"
     CDS             5021..5728
                     /locus_tag="ACHA02_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8670940.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
BASE COUNT         1955 a          903 c         1260 g         1647 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaa
//
LOCUS       DAYYDS010000129         5756 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000129 DAYYDS010000000
VERSION     DAYYDS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.134"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1778..2443)
                     /locus_tag="ACHA02_002667"
     CDS             complement(1778..2443)
                     /locus_tag="ACHA02_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8670941.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2569..4605
                     /locus_tag="ACHA02_002668"
     CDS             2569..4605
                     /locus_tag="ACHA02_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM8670942.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4609..5247
                     /locus_tag="ACHA02_002669"
     CDS             4609..5247
                     /locus_tag="ACHA02_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8670943.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5275..5703
                     /locus_tag="ACHA02_002670"
     CDS             5275..5703
                     /locus_tag="ACHA02_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670944.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1906 a          870 c         1050 g         1930 t
ORIGIN      
        1 caccccgttg ttatatgacc gtgttttgag aggaaccaat aaaaagcata ggctaactag
       61 gggttccttt ttcttttgtt tttttggaat ccgcttaagt gaaatgtatc tgaaatgaaa
      121 cggagataaa gtgatttttt gctataatga caaggtttta gctgtttcag ataccatgta
      181 acccaaaata gtttattgag aaaataggag agtatttgat actaactagt aggaaaaaat
      241 gagataaaaa ttaaatggaa tcatttcagg taatctcaaa taaaaaaata tacagtcctg
      301 tggcacttta cctatgggac tgtatatttt ttactctcag cgggcttgtt ttgactccgt
      361 gtctgactcc gtcgtattga ttttaaatga tattcgatga ccatttaaca ggtcattttt
      421 gaaatttttg tctctatatg actcagtttg ataattattg atagatcaat ttgggtctat
      481 atcgaacaag ggaataatcc aataaaaaat gatttagaat atcaaaataa caaaaaagag
      541 gactacctgt attcgtcctc ttttttgacc agattttgac cagtctaatt tacattctaa
      601 tatattcata tgagctaatt ttttgattaa atggtaagtt tttccttgtt tgttttagtt
      661 taaactagtg tgtaatttaa aaatctatat ggaagggtct gtggggtaat acttaaataa
      721 aggctaggaa cctgataaca tcaaggttcc tagccttttt ataataaaat gaagcgaaaa
      781 taatttcttt taaagagccg agattatcag ctatataaga gtgaatttga tgcaaaaaac
      841 agagccgaaa cagtcttggt gttcgtagct ctgcttatat gtttcgaatt taagacaaag
      901 agttggcaaa agatgttgat aaggaagata agataactgt gttatataaa ctaggtaact
      961 aactgtaaca agctaatttt aagagattct tattagaaga cgtttaggaa tataactaag
     1021 aaaatgatta tacaggtaat tgtcatttca ataatgtttt ctaattttcg cgtctttgtt
     1081 ttaagaaaaa ggccagaatc agttggaaaa aagttcgtac gatagtgaag aggggcaaaa
     1141 tttcctacta atattcccac taacaagaga taaaacggcc aggttggtgt gattcctcta
     1201 taaaaaaatg cccatgctac cgaaatccca tagattaaca actgaacaat ttttacaatg
     1261 aaaaaattgt tctgccatta ctcaaagtct cttcctcctt gtatcgttta tttgtttcat
     1321 tttatcatta tgataggcgc ttgaacaatc cattttgtat tttattgagt actcttggat
     1381 aaactaccag cgattatcat atttatttat gttactgtaa gccatcatat aaaagtaaat
     1441 gagcagatca tagaaatata aaaaagaact aaaattgatc taaattatgt gcagcttgac
     1501 tacgtaccaa tctatattac ttactagatt ggtcgttaat ctattcggat cgaatgagca
     1561 aaatattttt gtgaatgtcc atccttgatt atgattccaa aaagaatcat tgggaggctg
     1621 tggcgtaaat attatactat aagccagaaa tacctatcat tctgaacttt tttatttagg
     1681 aactttcatc tcaaggcttt ttcaattaat ttgtacccct gttttttgga taagatcatg
     1741 tgcattgatt ttttctatct ctacgttcat ctcctactta atgcctatct ggctcctgag
     1801 ggcttctttt taaggcgtcg tcatagtctt ggctgaggat ttcttggtaa tctgagggaa
     1861 tggcttccca agtgagcagg cgaacgttca tgacgtgttt tcctttgtgt ttgattacag
     1921 catggttttt ctctttttct acctctccag cgtgaaaaga cagccatgta ccatggccaa
     1981 agatatcatt acatacccaa cgttcgatgg aattgtccac gtatcctaca gggagtgtgg
     2041 tatagccgat ttgtggtttc aaaaaaggat tgaatcgatc gatcgtttca tttttgtatt
     2101 gataattgat aagaaaaaga tagaccaaac aaaagacgag cagaaagaaa gatagtgtct
     2161 ttttgagtga gaagggttgt tttgatgaat aagatttctt gttaacaaga ggttcatctg
     2221 gcaatgttgg ttctattgat tcggtagctg attcaataga aggaaaatca atcggcagac
     2281 ctaaagtatg atgtaaaagt gacagcattt caaggtctgg cattgtacga gaattttccc
     2341 aactagaaat cgttttatcg gaaacaccaa ttttttcgcc taattctttt tgcgtaagct
     2401 tacaatttct gcggtaagtt cgtatttttt ctccggtgtt catcttcttc tcctttcgat
     2461 tctacagtat atagatttat tctacatgac ctatacactt gatagatacg tttttttgat
     2521 gaaaaacggt atgattttat caaagaatca tttaagtaag ggagagtggt gataatcaaa
     2581 aaaaagttca cagttttact cacaatcata gtgaccggta tcatgagtgg ttttgccttg
     2641 gttagtataa ataaacaggt actctatcgg acaaatgaat ttttccatca aacagatact
     2701 tatactccga ttcgattgcc acaacatttt attgggcaag aagaatatga gcagctaatc
     2761 atggtatttg aaaatgtctc atcagaaact gagctttctt atatgaaaaa aacagtaaat
     2821 gaatccaatg aagtagatac atcttggaac ttcaagatac agccaaagct tgaaatgaca
     2881 ttgtattatc aaggggatgt caaatcttct cacgagaagg taccctattc taatacctcc
     2941 tttcgcgtaa cgtccattga agaatcgatc aaaataaaag attttgaagg tgaatttttc
     3001 ttgatgacga gagaaaaaga agcctatgaa cggttcattt cactattcac tgatcgattc
     3061 aatcaagcat ttcacactga ttatgccact aatatttttg aagactttga aaatagctat
     3121 tcgtattcgg ttatttcaaa tacagacgta tataattatc gagtgtattt aactattgga
     3181 ttgattcttt tattcctttt gttatcatcc tttttcttat tgatgaataa agaaatcagt
     3241 ttattgttga ataatgactt ttcattacat caagtattgt ggtgtttatt ggggaaaaag
     3301 aaaatcatta ttacattcat aattgcagga atcattggtg gttttatttt tatgtattat
     3361 aaggagcaat ttctttattt gttccagatg tacgttttct tgttcagcgg aatatatggt
     3421 atctctttga tttctctttt attagttgaa aaaatcgctt cgttaagaga agcgttcaac
     3481 tatatccaat acattctttt tacatttgga ttgttgtttt ctcttctttt gaccgttgcc
     3541 aatgtcgaat tagcaactat cgttttttct tcttcacagt tagctgtttc ccatgaacca
     3601 ccaaaagaaa tcgctggtga ggactatcat gtattttatc cagtaaccat cgggaaaaac
     3661 cacgtggatt ttatttattc caatcgtttt gcatcagcag cagatcagga attatatgaa
     3721 actttgaata aaaacggaag tatattagtc aatgtatcag attacttgaa tgaagaaaat
     3781 gaacagtttg gcagagggat aaaaataaat ctgaattatc ttaagaaatt tcctttgatt
     3841 gatgtctctg gaaggttgat ccaaataacc gaaaaagaaa cgcaccctgt tatattgatc
     3901 cctgaacgat atagacatac agatttaaaa aatgacctgg cgcaaataac agaatattat
     3961 caagaaatca gtgaaaaaga accgaaattt ctatttataa aaaacaatca acccatttat
     4021 acatttattc cttctatccc ctggatcgaa cattatccgg ttgttgaaat actgacattg
     4081 aaaaacagta tgtatttgga aagaaatatt ctcagcggag aaatctatcc accgttaaag
     4141 atcaagatag gctctttgtc gaaagaaagg ttatttgagc tgattgaaga aagtcagtta
     4201 cgtgataact tgcagttatc atttccgtat actcagactg aagagatagc tgtcaaagtg
     4261 ttaagcagat catttcatta cttgatacag atatttttac tcactttctt ttctttcttc
     4321 ttgttgagct atgtcatgtt atgcatatat tttgtctaca attgtcggaa aatcgctatc
     4381 tttcgttcaa gtggttattc gctctttgaa acgtataaag atttcttcat gatgaacctc
     4441 atcaaatggg gaacgacaag tgtaatcttc ctttttctta tagaaagaga gcctaaatat
     4501 ctttttaata tttttttctt tagcttcatt gattttattc tttcagttgt ttttattttg
     4561 tctacagaaa agaaaagtca gttattgctg atgaatggag gttaacaatt ggagccgttg
     4621 atagtgataa aaggtgcgaa aaaaatcatt cgaaacaaaa cgattcttca tttggatcgt
     4681 ttcactgcgg atgctgggga tcttgtgact gttgttggag aaagtggagc aggtaaaagt
     4741 acgttattga atatattagg attgaatgat cagttcagtt caggacaatt ctatttgttc
     4801 gatcaagaga cgaaacaatt aactgggaga gagaagcgct tgataaaaag aaataccata
     4861 tcttttcttt ttcaagattt tgggttgatt gaatatgaaa caatcgattt taatttagat
     4921 attggattga agttttcgaa tttaaatgtt tcagataagc gaacagcaaa gtatgaggca
     4981 ttgaaaaaag taaatataaa caagaagcta agtacgccta tctacacatt atctggtggt
     5041 gagaagcaaa gaatcgcctg tgcgagagtc ttattgaaga atagcaaaat cattttagcc
     5101 gatgaaccga ctgggtcatt ggataaaaga aataaggata aagtagttga aatcttatcg
     5161 caacaagctt cccaaggtac gttagttatc gtagtcacgc atgatccata tctgaccaaa
     5221 ataggaaaca agaagatcat gctttaaaaa tcaaaaagga aaggatgtcg ttttatgaaa
     5281 aaagcagtta ttatttgtgc actagggata ggttttgttg gaggaaacgc tatgtcatct
     5341 gaagtggaag caaattcagt tcacggtgaa atctattaca aatctttaaa gccaggagag
     5401 attcattttg atgctaaagg taatattatt aagtcaaatt taccagtaga ccgggaaatt
     5461 tctagaacag caattaatca tagctctgga agatgggttt attatagtga tacaacttgt
     5521 acgagaaaaa caggtcattc taaccactat agtcgtgtct ataatcgtca cggtgcaaaa
     5581 gcgaaagttg gaaactctat tcgatcagca aatgcaacaa aaggagtatg ggcctatgca
     5641 agtgcgacag gaaatagaac tgacacattc tcatgttggt acaatccaac cggttggtat
     5701 taagtatcca aaagtttata taaaatagaa aaaacatata atgaaacagc gttaaa
//
LOCUS       DAYYDS010000130         5749 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000130 DAYYDS010000000
VERSION     DAYYDS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.135"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="ACHA02_002671"
     CDS             complement(<1..1128)
                     /locus_tag="ACHA02_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8670945.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="ACHA02_002672"
     CDS             complement(1164..1874)
                     /locus_tag="ACHA02_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8670946.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2757)
                     /locus_tag="ACHA02_002673"
     CDS             complement(1942..2757)
                     /locus_tag="ACHA02_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFM8670947.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="ACHA02_002674"
     CDS             complement(2768..3736)
                     /locus_tag="ACHA02_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8670948.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>5749
                     /locus_tag="ACHA02_002675"
     CDS             4499..>5749
                     /locus_tag="ACHA02_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8670949.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT         1772 a         1183 c          915 g         1879 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagtcttgc caaagaacat tcgtagcgga
     4801 tacctctgtt gctgaaaaac actgtttcat tagtcaaaaa gttcgttggt ctgtcattgc
     4861 tcgcttaaaa gagaatactt ccatgacgga aatcgctcgt cagaaaaata tttccacttc
     4921 ttcggtctat cgtgtcatga agcgattcta ccgcccattg aatcctttca aacagacttt
     4981 gccaaaggtc ttatgctttg atgaattcaa atcggttcga agtgttgcca gtgcaatgag
     5041 ttttatcatg atggacggac aaagccatgc attattggat attgtcgaaa atcgacgctt
     5101 gccttattta gaacgctatt tttctcggtt ttcattagcg actcgtgaag ccgttcaatt
     5161 gattgttatt gatatgtatg cgccttatgt ttcgttggtg aaaaagcttt tcccgaatgc
     5221 tcagttgatc atcgatcgtt tccacatcgt ccaacatatt ggcagaactt tcttgaatca
     5281 tcgagtgaaa gaaaccacgg tacgacttaa aggaaattcc cactctcaac gagggttagg
     5341 gaaaaaattg aagcgctatt ggaaactcct acaaaaagaa gaagcaaagt taaactatga
     5401 aaaacgggtt tggcgtgcca gttttaaaga ttatttgact gaaagtgaga ttgccgatcg
     5461 actgttagct gcttgtccca acttacgcca aggctatcag ttataccaag acattcttta
     5521 tgcggtaaag aaaagagatc agtcactatt tgaggattgt ttaactagag acattagcgg
     5581 tctgccagaa acctatacaa ccactttacg aacgtttaaa aagtttctgc cacagatcca
     5641 aaatgcgctt cactacagct attcaaacgg accattggaa tgcttaaaca accacatcaa
     5701 agtattaaaa cgcaatgcgt acggctttcg caacttttac aatttcaaa
//
LOCUS       DAYYDS010000131         5611 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000131 DAYYDS010000000
VERSION     DAYYDS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.137"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..2008)
                     /locus_tag="ACHA02_002676"
     CDS             complement(59..2008)
                     /locus_tag="ACHA02_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8670950.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="ACHA02_002677"
     CDS             complement(2050..3108)
                     /locus_tag="ACHA02_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFM8670951.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="ACHA02_002678"
     CDS             complement(3227..3682)
                     /locus_tag="ACHA02_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670952.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="ACHA02_002679"
     CDS             complement(4266..5069)
                     /locus_tag="ACHA02_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8670953.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2073 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat a
//
LOCUS       DAYYDS010000132         5578 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000132 DAYYDS010000000
VERSION     DAYYDS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.138"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      130..296
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            444..1001
                     /locus_tag="ACHA02_002680"
                     /pseudo
     CDS             444..1001
                     /locus_tag="ACHA02_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1059..3098
                     /locus_tag="ACHA02_002681"
     CDS             1059..3098
                     /locus_tag="ACHA02_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8670954.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3207..4358)
                     /locus_tag="ACHA02_002682"
     CDS             complement(3207..4358)
                     /locus_tag="ACHA02_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8670955.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4512..5042)
                     /locus_tag="ACHA02_002683"
     CDS             complement(4512..5042)
                     /locus_tag="ACHA02_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8670956.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5141..5491)
                     /locus_tag="ACHA02_002684"
     CDS             complement(5141..5491)
                     /locus_tag="ACHA02_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8670957.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2017 a         1023 c         1041 g         1497 t
ORIGIN      
        1 gattctttca acttatttgt tatttttttc taaaaaatat taaagattgt taaatttcac
       61 tatttcattt caaattccat tgcctaatca ttaggaaaag tctatcatga actcgtaaat
      121 agaatatagt aataaccttg ttaggtgagg ctcctataca aacataggct gttgcccaga
      181 aacgtcgaaa gtcgccaatg ggtaaaacag aaactgttgg attaaggctt tttccaatag
      241 ctaagaatgt tttctttacg ttgtatagtg ctaaaactcg gacgatgaga tgatgctagg
      301 tcagaattct tgttcgaaaa ggtttctgac cttttcttta tatttttgtg actgacttat
      361 ttgatgagac aaactcgctc ctcacctgac aagtggattg ctgtgtgagg aggagatgcg
      421 gtgaaaaaag atcgattggt ggaggtcgct cggattcact tctggttgca caatacaacc
      481 agaaaacaca acaagcaaaa ctgatctcta tcatgagaga ttcatatgtc gatattccag
      541 gttacggaat gaataagata aatgcagctt actcttatgg gggagttgat ttattaaatc
      601 aaacattaaa ggaaaatttc aaatttgaag ctccctatta tgcaagtatc acatttcaag
      661 attttatcga ttgcgttaat gaactgtttc ctggtggagt caagatcgat gcagaaaaat
      721 ctctagattt agatggcgta tatataaaca aaggagagca agtgatggat ggtaatactt
      781 tactccagta tgctcgattt cgggaagaca aagaaggaga cttcgggagg atcagaaggc
      841 agcagcaagt gatcaaagct atctctcagc agctgaaaga cgtaacttca atattcaagc
      901 tacccaaagc ggttgggaaa ttacttggta gtatccaaac gaaccttcca gaaagtgtgc
      961 ttctggattg tggtatggat ttcctcaaag acatatgtta gaataaacag gtataaatag
     1021 tgaaataaag gaatgacaag caagagaagg aggaaaaaat gaaaagaagt gacaagcacg
     1081 gcaaaaatcg aacaggcgct tatattgccg gcgcagtgat cttaatagca gctgcgggtg
     1141 gcggttattt ttactaccag cactatcaag aaacccaagc agtagaagct ggagaaaaga
     1201 cagttgagca atttgtccaa gctttgaaca agggagatta taacaaagct gcagaaatga
     1261 catcaaaaaa ggcggcaaat aaaagtgcat tatctgaaaa agagatctta gataaatacc
     1321 agaatatata cggtgctgct gatgtcaaag gacttcagat atcgaatcta aaagtagata
     1381 aaaaagacga ttctacttac agtttttcat ataaagcaaa gatgaatacc tcattaggtg
     1441 aattgaaaga tctttcttat aaaggaacat tagacagaaa tgatggtcaa accacgatca
     1501 actggcaacc caacttggtt tttccagaaa tggaaggaaa tgacaaagta agcctgacca
     1561 cacaagaagc agcaagaggg aatattatag atcgaaatgg tgaaccatta gcaacaacag
     1621 gcaaactaaa acaattagga gtcgttccaa gtaaacttgg agatggaggc gaaaaaacag
     1681 ccaatatcaa agcgattgct tcctccttcg acttaacaga agatgctatc aatcaggcga
     1741 tttcacaaag ctgggtacaa cccgattact ttgtcccatt gaaaatcatt gatggagcaa
     1801 cgccagaact tccagctgga gctaccattc aagaagtaga cggcagatat tatcctttgg
     1861 gtgaagcagc tgctcagctg attggttacg tgggagatat cacagcagaa gatattgata
     1921 agaatccaga attaagcagt aatggaaaaa tcggtcgctc aggtttggag atggcttttg
     1981 ataaggatct tcgtgggaca acaggtggga aattaagcat tacagatgca gacggtgtag
     2041 agaaaaaagt tctgatcgaa catgaagttc aaaatggcaa agatatcaaa ttgacaatcg
     2101 atgcgaaggc acaaaaaata gcttttgaca gtctaggagg aaaagctgca tcaactgttg
     2161 cgacaacgcc aaaaaccggt gatcttcttg cgcttgctag ctctccaagc tatgatccaa
     2221 acaaaatgac aaacgggatc tcacaagaag attacaaagc ttatgaagaa aatcctgaac
     2281 atccattcat cagccgattt gcgacaggtt atgctcctgg atctacgttt aaaatgatta
     2341 cagcagcaat cggtctcgac aacggcacta tcgatccaaa tgaagtgttg acgatcaacg
     2401 ggcttaaatg gcaaaaagac agttcttggg gatcttttaa aacaacgcgt gtcagtagtg
     2461 atgtatcaca agtagactta aaaactgctt tgatctattc cgataatata tatgcggcac
     2521 aagaaacgtt gaaaatgggt gagaaaaaat ttcgtacagg cttagataaa ttcatttttg
     2581 gtgaagacct tgatttgcca attagtatga atccagcaca aatttctaat gaagatagct
     2641 ttaactcaga tatcttgcta gctgatactg gatatggaca gggcgaactt ctaattactc
     2701 ctatccagca agcagcaatg tattctgttt ttaccaacaa tggcacactt gtctatccta
     2761 aattgattgc agataaagag acaaaagata agaagaatgt catcagcgaa acagcagtac
     2821 aaacgatcgt gccagatctg agagaagttg tgcaagatgt aaatggtaca gcacattctc
     2881 tttctgcttt agggattccg ttggcagcga aaactggtac agcggaaatc aaagaaaaac
     2941 aggatgtaaa ggggcaagag aacagtttct tgtttgcttt caatcttgat aaccaaggtt
     3001 atatgatggt cagcatgttg gaaaataaag aagatgatga ttcagcaacc aaacgagcat
     3061 cggaactatt gcaatacctc aaccaaaatt atcaataaaa aattgctttt taagtacagt
     3121 aaaaggactg tggcgttatc gtcacagtcc ttttttcaaa taaacggttt tcacgatgtc
     3181 tctttcacaa cttctttttg atttactcac acttcataaa ctttttttgt aaaatatttg
     3241 agaacaagtg gagaaataag tgtacttaat aaaatcacga taactaaagg tgaatagtac
     3301 tgtggttcga tcaattgact ttgctgtcca atttgtaaaa tgatcaaagc catttctcca
     3361 cgcgaaatca taccagcacc gaccatcaat gaactatgtt gagaaaaacc ggcaatctga
     3421 gcaccaaaat aacctccaag caatttggtc aaaatcgcta caagcgtcaa tatagaaata
     3481 aagagtagtt gttcactaaa gtcagaaaaa tcaacttcta atcctacact gacaaaaaat
     3541 actggtataa atactgcata tcctaaagcc tctacattgt ggtatacttc ttctttaacc
     3601 tttgtctgac tgacagctat cccagcaaaa aaagcaccaa tgacagagct aagcccaaca
     3661 agatccgcta cgtaagacat tcctaaacaa ataactagcg atacgatgat aatagaagaa
     3721 tttgcgtaca ctttttctgc aaatgaaagt aggaaaggcg caatccattt gactaataag
     3781 aaaataaaaa caaaataaat tagctgttca atcaaaacaa gcggtaaaga agcattgcta
     3841 gaagcttccc ccgttagaaa agataggctg aaacttagaa ctaaaacgac taaaatgtca
     3901 tctaccacag aagcacccaa aattgttgat ccttcttttg tattcactac gtttagttct
     3961 tttaatactt ctactgaaat actgacagaa gttgccgcaa gaataatccc aaagaaaatc
     4021 gcttcatttc ccgaaacttg gaatgctttg cctgtcaacc agccaaaaac aaccgggaag
     4081 atgatcccaa gcaaagcaac gaacatacca ggtcggaaat attttttcaa aagagataaa
     4141 tcactttcca gaccagctaa aaacattaat aagatcacac caatctccga aaagtcatgg
     4201 actagaatat caggtttgac ccaattcaat ccagcttggc ctagcaaaac accaacaagt
     4261 aattgtccaa tcactgctgg gatacctaat ctccgcgaga tatgtgagcc aatcgttgtt
     4321 gaaatcaata tcaggcataa aataccaata aattccattc tcatcattcc tttgtataaa
     4381 atatagcccg aacttagcat aacatacatt attgaaaaaa taggaaaata tactcacaaa
     4441 aaaaccaaca atccaaaaga tttttttgga ttgttggttt ttatacggtc tattttaata
     4501 actaaaaatg attaacgtat agtcattttt cttattctga tgaaattgat aatctcctgg
     4561 atgcccatga aaatcaaaat aaagccgaat atttggaata gaagcatcaa acttttgaat
     4621 ggattgaaca ataagacgaa tccgccaatc aacaacagaa tactactata aacactccaa
     4681 cttttagctt ttgtattcat gctgaagaac aactggaaca agctgttgat gataacagct
     4741 aatccaagaa gaatcggcag gatagaaacg attgcaggtg caaaagcata gacgatcaga
     4801 gctaagatca ggaaaaaaag accactgaac agaccgatac tccaagaacc tgttttcctt
     4861 ttaaaacggt aagcctcaaa aagattcaac aacccaagca aaacaagata ggcggtgatc
     4921 agataaccga taaaatgaaa aacagcagaa gggtttagca caatcgcaat cccagaaata
     4981 atataaacag cagctcttaa taaggcgtat ttttgaatat tttgtttaat cgtctcaaac
     5041 atataagaaa acctcctttg acttatttta acgtgaacta gtcattttac caaattaaat
     5101 gttacaaaat gagcgaagga gaagaatatc tctggaaaaa tcaacttctg aggatctttt
     5161 ccattttttt gccttttgct agctcatcga tcattttatc taaataacga atttctttca
     5221 tcaagttatc ctcgatgtct tctactcgta caccacaaac aacacctgta atcaaggaac
     5281 gtgaaggatt ggggacaggg gcttccgcaa aaaattgttc gaaagtcact tgtttttgta
     5341 gttgatattc tagttctgtt tgggaataac ccgtcagcca acaaatgatt tcatctactt
     5401 cctcttttat acgatctttt ctctccgctt ttgctacata cataggataa acgcttgcaa
     5461 aacttgtcgt aaaaatacga ttttgcgcca taatcagtca cctttctttt tttcaaatta
     5521 tttactactt atagtatata agaaaaagag aagaaaaagc gattattcaa aaaaacaa
//
LOCUS       DAYYDS010000133         5532 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000133 DAYYDS010000000
VERSION     DAYYDS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.139"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..985)
                     /locus_tag="ACHA02_002685"
     CDS             complement(<1..985)
                     /locus_tag="ACHA02_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFM8670958.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACHA02_002686"
     CDS             1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACHA02_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFM8670959.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="ACHA02_002687"
     CDS             1810..2964
                     /locus_tag="ACHA02_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFM8670960.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="ACHA02_002688"
     CDS             3179..4147
                     /locus_tag="ACHA02_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM8670961.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="ACHA02_002689"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="ACHA02_002689"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM8670962.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5532
                     /locus_tag="ACHA02_002690"
     CDS             5177..>5532
                     /locus_tag="ACHA02_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFM8670963.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRG"
BASE COUNT         1669 a         1078 c         1252 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc gg
//
LOCUS       DAYYDS010000134         5352 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000134 DAYYDS010000000
VERSION     DAYYDS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.140"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..540)
                     /locus_tag="ACHA02_002691"
     CDS             complement(<1..540)
                     /locus_tag="ACHA02_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8670964.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEIN"
     gene            complement(530..1729)
                     /locus_tag="ACHA02_002692"
     CDS             complement(530..1729)
                     /locus_tag="ACHA02_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8670965.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1859..3235)
                     /locus_tag="ACHA02_002693"
     CDS             complement(1859..3235)
                     /locus_tag="ACHA02_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8670966.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3235..3891)
                     /locus_tag="ACHA02_002694"
     CDS             complement(3235..3891)
                     /locus_tag="ACHA02_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8670967.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3901..5178)
                     /locus_tag="ACHA02_002695"
     CDS             complement(3901..5178)
                     /locus_tag="ACHA02_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8670968.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(5197..>5352)
                     /locus_tag="ACHA02_002696"
     CDS             complement(5197..>5352)
                     /locus_tag="ACHA02_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8670969.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1672 a          983 c          680 g         2017 t
ORIGIN      
        1 attgatctca tcttctaatt catcctgata ggctttcgct tcatcatggg tcatttcttt
       61 cgtaaactta tgtttattgg cgttagcttt gatgtgggta gaatccagat acaaattatc
      121 ctctgttaca aaacctgctt taactgcttg ttctaaaata taggcaaaaa tatcctcaaa
      181 caccgtcgtt tctctaaatc ggcgaacata attctttcca aaagttgaga aatgggggat
      241 tttctcctcg aaggagtagc ctaagaacca gcggtaagcg acattggtat cgacttcctt
      301 aatggtttgt cgcatggaac ggataccgaa taaatactga ataaacacca atttgatcag
      361 aatcactgga tcgatacttg ggcgacctaa atcggagtaa gtggcttcga cgattggata
      421 aataaattca aaatcaagcg cggcatcaat ctttcgaacg agatgatctt gaggaactag
      481 atcatcaatc gtaagcatcg caaattgatt tctaccattc gtattgtttt tagacatcat
      541 agctatcact ccttgcattc gttttcataa atctattata aaagaaaaga ctgtagacat
      601 catccataaa atggactttg tctacagtct gcgcattctc attagagaac actccctttt
      661 ttgtgacttt tctgaagcta gagtgaatac tttcaactgc attagtagtg tacatgattt
      721 ttctaatggc gctgccataa tcaaagagtt gttcaacatg agaaaagttt cgtttgcata
      781 catctactgc accagaataa tgagaccacc gattttgaaa actgtcgaag gcagcgtgtg
      841 cagcgtttaa agaagaagca ctgtagaatt ttttcatgtc tcgacagact tccttataat
      901 ccttacttgg gaggtatcgt aacgcatttc gaacaagatg aacaatacag cgctgcacaa
      961 tgacagaagg aaaaattgct tttgcgcctt ctttgagacc ggaaacaccg tccatcgaaa
     1021 tgaaaaaaac atcttctaca ccacctactt tcagttcatc aaatacttgc atccagcgat
     1081 ttttagattc tgtttgattg agccataggc ctaaaatctc cttgtttcct ttaagatcat
     1141 agccaagaat ggtgtacaca gcatattctt tagcttcata gttttcgcgt aaagtgacgt
     1201 acatacaatc aacgaataga aatgcataac acttggctaa tggccgagct tgccattctt
     1261 ccaattcagg aaggacggca tccgtgatat cagaaatcat ttcgtgggaa atatcaaagc
     1321 cgtaaatatc ttcaacggtt gcggcgatat cgcattgact cattcctcgt gcatacatgg
     1381 aaagaacctt accttcgatg tcggagacat ctcgttttct attaggaatc aactcaggtt
     1441 caaaagaagc ctcccggtct ctgggaacat caatgtttac tttaccaaag ctcgttttaa
     1501 gcgtcttatt gccataaccg tttcgtcgat tgtcatgttt ctttggctct ttagaatggg
     1561 cgtcataccc taaatggtta ttcaattctc cttgaagcat tttttcaaat aagggaccaa
     1621 acacatcttt taaaacatct tgcatatcct ctacagattc gggttgataa gtattcaaaa
     1681 tggattcagc taacttctca gcatcaggat ttcttttctt tctagccatc gtgtaatcac
     1741 ctcttgatct tattttagaa aaataaaaac cgtgaagcaa ccacccgcct aggattgatg
     1801 gttacttaca cggtttatat tacactctca tttttggtgc taaaaagcac cgaaaatttt
     1861 aattattgtc aattaataat tcttttggat tccttcttaa tatattgaat gaagaaataa
     1921 tcaatgatac aagaagtact aaactcataa atataactac ataaatcatg aatttaggta
     1981 atacattgat atctaaactt gataatgtct tgttaaatcc ttcagcttct gccccaccac
     2041 ctaattggct agatgcagat tgtttagcta tttgtttagc tatatcacca gtaaccttat
     2101 tcaatatatt attcccaagc ttgtcagctg tatattgagc taagaaatag gaaccaacga
     2161 atgcggggat tgatataaat accatctcaa ttaggaattg accaaaaatt tctaattttg
     2221 atatacctaa tgatagtaat actgctattt ctttctttct agcattcatc cataagaata
     2281 ataataatga aactacaact cctgcaaata ttaatgagcc aaaaaataat ttatttgaaa
     2341 ttgagtaaat acctgatata gattgttgta atgcagggta atttgatgaa cttttaatca
     2401 aattatattc tctccaattt atatctaatt tcccaagatc ttttattaca ctatcaagat
     2461 tcttatcacc ttttacaaag aatgttgcat cttggtatac agctgtatct tcggtattac
     2521 cataaacttt agcagctgta tgaatatcag tgattaatgt attttcgtat agttcttgtg
     2581 ctgcacttac tccaccgcta ttatgcccat cgaatagacc tttaatttct acttctactg
     2641 tttcatctgc acctttctca ttatcagcat caaataaatt agattttatt tttatcttat
     2701 ctccaacttt aaggttattt ttttcagcta aatctttatg cattaagatt ttatttttat
     2761 cttcattttc taaatgcttt ccttctacta atttatatgc ttctgataca aattttgttt
     2821 cttttgttga gtcgttaacc ccagttaaca taactgttct tttaaaattc tttgctcttt
     2881 caggtgattg attcgcaaga gtctcttgag tttcaataat atcataatca actaaatctg
     2941 caacactgtt tattcttttt acataggagt ctatactatc tgtttgagat atctttttaa
     3001 tatcttcacc ttttacattt cccccacctc taggtgttcc tggatttact tgtctattta
     3061 tctccataga aaaactattt gttatattag caaatgtttc ttttgaagct ctgtccgtag
     3121 catctttaat agataaacta atgagactta aagttgacat agataaaata actaatatga
     3181 taattaatga ttttaaactt tttctagtta cataagcaaa tgcatttttt atcattattc
     3241 aacctccaaa ttcattttat ttactttttt caacttttta ccacttagtt ctaaaatgat
     3301 atccgcagaa tctgctactt ccttactatg tgttacaact attacacatt tatttctatc
     3361 tttagctaat gtctttaata tattaattat ttctccagca gtaacactgt ctagattacc
     3421 agtaggctca tcagctagta ttattggagc atctgatacc aatgctctag caatagctac
     3481 tctttgttgc tgtccaccag ataatttcat aacatttctt tttatttgtt ttttatctaa
     3541 acctagttca aacaagatac tttcatctgc tgatttattt actaatctaa tattttcaat
     3601 tggtgataaa taatctatta aattatagtt ttgaaatact aaagatatat tattttttct
     3661 atgattacta tatcctttat tttgtatatc ttcattctta aacaatattt ttcctgtttg
     3721 aggtttatca agtccagcaa gtaaggaaag aagtgtagat tttcctgttc ctgactttcc
     3781 tactatcgca taaaacttcc caagttcaaa tttttgattt actcctgaca aaactttttc
     3841 tttagaattt gcataactat atgctacatt ctttatttct aatatatcca ttattttctc
     3901 ctaacttatt tttgataaga tttctttagg cttcttgatt aacattaatg aagaagcaaa
     3961 tacaactgat aaaacaataa tacttattaa tattaaataa ctttgtccaa gtgttgttat
     4021 atttgacata aaactactat tatttattaa acttccaccg gaaatcattg aatcctctga
     4081 gttaatgaat ccatctacaa ttacttttag taatacattt cctaaaaata aggaagatat
     4141 tatacttggt atcgatatga atattaactc gaatataaat tgcattataa tttgtatctt
     4201 agatgttcca atagataaga atatacctat ttcataaatt ctttccctta accatagaat
     4261 caagattaat gaaagaacaa ccatcccacc taacataata gaataagtca ttacttttat
     4321 tatatatttt attccactca ctgactctaa agactcttca aacgcattag aatctttttc
     4381 aacaaaatac tttgactcat caattttaag ctctttcaat ttgtttaagg ctaagtctgt
     4441 agattctgca ctaccagaat acattaaaat tttatttgca atttcattat tttctgaatt
     4501 atttaatatc tcttggcttg ttgaataatc tacaaacacc atattttcac taaaatcaga
     4561 agataatcct gtatatgttt cctgtttttt accagaaaag atccctataa ttttaaattt
     4621 atgacttttt atttttccac ttttttcaat atctagtaat tcaagatcaa cttcatcacc
     4681 taattttaga ttgttttgtt tagcaaattc ttcatgaaca ataattgaat tcttatcatt
     4741 ttcttctata tttttacctt ctttaattgt aaatactcca ctactaaata aaatatttct
     4801 tttagtatta tttgtagctt cgagtgaaac aacattctta aattcgtcag ataaatcttc
     4861 tctatttatt ctttgttctc cgctaactac tttagcatct tttagttttg ctaatccatc
     4921 atattgaatt attttttctt caatttcttt taatttttca atatctttaa attgattaac
     4981 attaaaatat ttaccatctt tttttgttat tgatattgaa gaattagaac tttcatataa
     5041 agccttttct atttcattgc ttgatttcat tattgttaaa caagaataca agcaagaaag
     5101 aactatagtt aatatgataa aaattattaa agttctattt ttctttcttg taatatatgc
     5161 tatagcattt tttatcactt tcctaactac tcctctctat tttcttactc cataactaat
     5221 tgcatccact ggacaagcat ttttacatcg tccacatctg atacactcca gatgattgca
     5281 attctcaact gaatctttat tcatttggca aactttttta cattttccac aattaataca
     5341 cttatcctta tc
//
LOCUS       DAYYDS010000135         5341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000135 DAYYDS010000000
VERSION     DAYYDS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.141"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..285)
                     /locus_tag="ACHA02_002697"
                     /pseudo
     CDS             complement(61..285)
                     /locus_tag="ACHA02_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(839..925)
                     /locus_tag="ACHA02_002698"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ACHA02_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2091..3131
                     /locus_tag="ACHA02_002699"
     CDS             2091..3131
                     /locus_tag="ACHA02_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8670970.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3955..4287
                     /gene="traE"
                     /locus_tag="ACHA02_002700"
     CDS             3955..4287
                     /gene="traE"
                     /locus_tag="ACHA02_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8670971.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4906..5247
                     /locus_tag="ACHA02_002701"
     CDS             4906..5247
                     /locus_tag="ACHA02_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8670972.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1770 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtggtat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgttct gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cacaatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     3961 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     4021 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     4081 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
     4141 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
     4201 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     4261 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     4321 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     4381 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     4441 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     4501 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     4561 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     4621 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     4681 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     4741 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     4801 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat
     4861 ataaaacata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     4921 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     4981 ggatttgtga cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattagac
     5041 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     5101 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     5161 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     5221 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     5281 tagaaggagt cacgatgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
     5341 t
//
LOCUS       DAYYDS010000136         5261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000136 DAYYDS010000000
VERSION     DAYYDS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.142"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..1066
                     /locus_tag="ACHA02_002702"
     CDS             59..1066
                     /locus_tag="ACHA02_002702"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8670973.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1078..2082
                     /gene="neuB"
                     /locus_tag="ACHA02_002703"
     CDS             1078..2082
                     /gene="neuB"
                     /locus_tag="ACHA02_002703"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8670974.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2079..3227
                     /gene="neuC"
                     /locus_tag="ACHA02_002704"
     CDS             2079..3227
                     /gene="neuC"
                     /locus_tag="ACHA02_002704"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8670975.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3200..3877
                     /locus_tag="ACHA02_002705"
     CDS             3200..3877
                     /locus_tag="ACHA02_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8670976.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3828..5009
                     /locus_tag="ACHA02_002706"
     CDS             3828..5009
                     /locus_tag="ACHA02_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8670977.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5022..>5261
                     /locus_tag="ACHA02_002707"
     CDS             5022..>5261
                     /locus_tag="ACHA02_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8670978.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKE"
BASE COUNT         1953 a          679 c         1034 g         1595 t
ORIGIN      
        1 ttttttaaag attaagaatc gaattataga tgactataat tcataggagg agatataagt
       61 gtttaaagga aaaacaatat tagtaactgg cggaactggt tccattggaa gtgaaattgt
      121 tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt tatagtagag gtgaagaaaa
      181 acaattttat atgcaacaag agctctcaaa tcacactaat ttgagatttt taattggtga
      241 tatcagagac tctcaaagga ttgattatgc atgtcgtgat gtggatatta tttttcatgc
      301 tgccgcaatg aaacacgttc cagcaagtga atataatcct atggaagctg taaaaacaaa
      361 tgtgatggga actcaaaatg taatagatgc agcgatcaaa aataatgtaa aacattttgt
      421 aggtatcagt acagataaag tagtgagtcc tactaacacc atgggtgcga caaagttatt
      481 gagtgaaaaa ttaataatag ctgccaatca gtataaagga aaaacagaaa caatattttc
      541 ttgtgtaagg tttggaaatg tcatggggtc aagtggatct gttattcctc tttttagtaa
      601 acaaataaaa aacaataaag atattacttt gacagatgtt gatatgacac gatttatgat
      661 gtcaataccg gatgctgcaa gattagttct gtctgctgct atgaattcaa aatttggaga
      721 aacatatgtc ttaaaaatgc cggcaatcaa tattttaact ttagctcaat cattaataaa
      781 aatatataat gatacacatg atgaaaaata tagaggtgaa ataaaagttg taggtatcag
      841 gccaggagaa aaattatatg aagagctaat gactacagac gaaattgaac gctcatatga
      901 aaatgataaa atgtatatca ttccaccaca ctatcatgct actgacgaac tgtacaaaga
      961 ttatgaaaaa attaaaatca ctgcatactc ttcagatgca acagaattaa tgagtggaca
     1021 ggaatttatt agaatcgcaa aagagaataa tgtcgtgact tggtaaggag ttttatgatg
     1081 aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc ataatggaag tctagattta
     1141 gctttagaaa gtattgataa agctaaagaa tgtggagcta acgcggttaa atttcaaaca
     1201 tttaaaacga ataagctagt ttccaaaaag gctccaatgg caaaatatca gaaggataat
     1261 cttaaagagg acagttctca atttgaaatg ctaaaaaaat tagaattatc tcaatctgat
     1321 tttattagaa taaaagatta ttgtatacaa aataaaattg attttttgtc tactcctttt
     1381 gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa aattggctca
     1441 ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt gccagtttta
     1501 ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg tttcatagat
     1561 agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga agatattaat
     1621 ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta ctccgatcat
     1681 tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt aattgagaaa
     1741 cattttacct tggataagga actacctggt ccagatcata aagcttcttt agatcctcag
     1801 gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg tgatggtgtt
     1861 aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag cctagtggtc
     1921 actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat aaaacgtcct
     1981 ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt taaaagacca
     2041 ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata cttatagtaa
     2101 gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta ctacaagata
     2161 attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat ggatttacta
     2221 aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt ttgtttttag
     2281 agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt ttcacaaata
     2341 tatttgaaca aactagccct gattttgtat tattactagg ggatagagga gaaatgttgg
     2401 cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac ggaggcgaag
     2461 tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca tctgttcatt
     2521 ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat tcttggagag
     2581 tttttcccgt cggagctcct agaatagata ctattttaaa taatagatta cctagtttta
     2641 gagatataaa agaaaaatat catctaaaat ttgaaaagaa aaaatacgtt ttgcaagtat
     2701 ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca aattttagct ttgatcaatt
     2761 caatggaaca acaatctcaa ccaattattt gcattttacc taatagtgat gctggtagcg
     2821 aaattatcag aaaaatgtac cagaaatatt taaaaacgga tattatttat attacgaatt
     2881 tagagccgga ggaatacctg actatattaa aagagtgcaa atttatgatc ggaaattctt
     2941 cttctgggat aattgaagct gcatcattca aaataccagt aataaattta ggaaccagac
     3001 aaaatggacg agaacaatct aaaaatatta taaatgcaaa tctaaatgaa aatgaaattg
     3061 aaactgcttt gaataaaata caagatcctc agtttataaa aagcttgaaa aatgttaaga
     3121 acatctacgg tgacggcaac agttctagaa gaataacaaa aacattgaat caaatattga
     3181 atgatgggga agatattaag tggattcaaa aacaaatagc ttattaaaat atactagtga
     3241 tccatacatt attattgggg ctggcggaca cgcaaaagtc attattgaaa ccttaagatt
     3301 agaaaataag ttaatatatg gattttttga tgataagcaa aataaaatgc aagatctgaa
     3361 atatcttggg aaaataaacg aattgaatag ctgggcggat agcaaaaaaa aaatctataa
     3421 atttattgta gctattggtg actgtgtagt aagaaaaaag attataaaaa aattgaatat
     3481 gccaagtgag caatatggaa tagctgttca tccaagtgct atagtggcaa aagaaatttc
     3541 aattggttat ggaactgtta tttttgcgaa tgctgtaatt aacccagatg ctgtaattgg
     3601 agagcatgct ataataaaca caggtagcat tgttgagcat gattgtagaa ttaataacta
     3661 tgttcattta tctccaggtg tatgcttagc gggtggagta catgtaggtg taggaactca
     3721 aataggaatt ggttctcaat gtatccagat gaagaaaatt ggatcttggt gtatgatagg
     3781 cgcagggagc actattgtta aggatattcc ctcacattcc ttagcttatg gcaatccagc
     3841 taaaattaag aaagaaggaa ttaattttga attctagaat tttattatca agtcctcata
     3901 tgggaggaac agaagaaaaa tatgttgcta aggcttttaa agataattgg atagcgccat
     3961 taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa atatactggt tctaagggcg
     4021 gagcttctgc gttaagttca ggtacagctg ccatacatct tgccttaaga ctgcttaatg
     4081 ttggaaaaaa cgatattgtc tttgctcctt catttacttt tatagctaca gtaaatccta
     4141 ttttatatca aggggctgta ccagtgttca ttgactcaga acctaataca tggaatatga
     4201 gccctgttgc actagagagc gcattagaag aagctgaaag aaacaaccaa ttacctaagg
     4261 caatcatcgt agtgaattta tatggtcaaa tggctgatta tgaacgaatt ttagctattg
     4321 caaaccaatt taatgttcca gtgatagaag atgcagcgga atcgttaggt tctacttata
     4381 agggaatttc gtcaggctta tttgggacaa ttgggatcta ttcatttaac ggtaataaga
     4441 tcatcacaac ctcaggtgga ggagctctag tgtctagtga taaaaaatat gttgagcatt
     4501 caagattttt agcaacacaa gcaagagatc aagcaccgta ttatcaacat agcgaaatag
     4561 gttataatta ccgtctaagc aatatttctg ccggtatcgg acgtggacaa atggaagtat
     4621 tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg atataaggaa gctttttctg
     4681 gaattgatag aattgaaatg atgccagaag tggatggcat ggtgccaaat cgttggttgt
     4741 ctactttaac gatcaatgat tataattcta ttgatcaagt agttaacgta ataaataaac
     4801 taaatgaaaa aaacattgaa acaagagctt tgtggaaacc actacatttg caaccagtat
     4861 ttcaaggggt aaaaatgtat taccatgata ataatcttag tgtgtcagaa aagttattta
     4921 atagcggctt atgcttacct tctggaagta atatgacaga tagtgaacaa gaatttgtta
     4981 ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt tatgaacata ggaattattg
     5041 gtttaggttc catggggaaa agaagactta gactcattca agaacacttt aatgatattt
     5101 tactatgtgg tgttgatact aatgaggcga gacgaaagga agttgaaaag gaatttggaa
     5161 taccaactta taatagtcta acagaggcaa aaaatttgtt tgaaataaat tcagtttttg
     5221 tttgtacatc ccctataacc catgaattta ttataaaaga g
//
LOCUS       DAYYDS010000137         5220 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000137 DAYYDS010000000
VERSION     DAYYDS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.143"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="ACHA02_002708"
     CDS             complement(<1..1256)
                     /locus_tag="ACHA02_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8670979.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="ACHA02_002709"
     CDS             complement(1270..2487)
                     /locus_tag="ACHA02_002709"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8670980.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="ACHA02_002710"
     CDS             complement(2477..4072)
                     /locus_tag="ACHA02_002710"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8670981.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="ACHA02_002711"
     CDS             4197..4982
                     /locus_tag="ACHA02_002711"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFM8670982.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            4975..5217
                     /locus_tag="ACHA02_002712"
     CDS             4975..5217
                     /locus_tag="ACHA02_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670983.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DAYYDS010000138         4944 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000138 DAYYDS010000000
VERSION     DAYYDS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.144"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACHA02_002713"
     CDS             <1..649
                     /locus_tag="ACHA02_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670984.1"
                     /translation="DTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLS
                     AVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMR
                     YQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITM
                     IHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="ACHA02_002714"
     CDS             708..1148
                     /locus_tag="ACHA02_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670985.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="ACHA02_002715"
     CDS             1160..1828
                     /locus_tag="ACHA02_002715"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8670986.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="ACHA02_002716"
     CDS             1892..2515
                     /locus_tag="ACHA02_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8670987.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="ACHA02_002717"
     CDS             2571..2957
                     /locus_tag="ACHA02_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8670988.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="ACHA02_002718"
     CDS             3170..4561
                     /locus_tag="ACHA02_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8670989.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1634 a          829 c         1054 g         1427 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtg
//
LOCUS       DAYYDS010000139         4788 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000139 DAYYDS010000000
VERSION     DAYYDS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.145"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            564..989
                     /gene="bacA"
                     /locus_tag="ACHA02_002719"
     CDS             564..989
                     /gene="bacA"
                     /locus_tag="ACHA02_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFM8670990.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="ACHA02_002720"
     CDS             1019..1183
                     /locus_tag="ACHA02_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670991.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="ACHA02_002721"
     CDS             2141..3118
                     /locus_tag="ACHA02_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8670992.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="ACHA02_002722"
     CDS             3128..3625
                     /locus_tag="ACHA02_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8670993.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="ACHA02_002723"
     CDS             4001..4282
                     /locus_tag="ACHA02_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670994.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="ACHA02_002724"
     CDS             4284..4700
                     /locus_tag="ACHA02_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670995.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAYYDS010000140         4773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000140 DAYYDS010000000
VERSION     DAYYDS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.146"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            615..1526
                     /locus_tag="ACHA02_002725"
     CDS             615..1526
                     /locus_tag="ACHA02_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HFM8670996.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2013..2279
                     /locus_tag="ACHA02_002726"
     CDS             2013..2279
                     /locus_tag="ACHA02_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670997.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2351..2692
                     /locus_tag="ACHA02_002727"
     CDS             2351..2692
                     /locus_tag="ACHA02_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8670998.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2747..3166
                     /gene="ssb"
                     /locus_tag="ACHA02_002728"
     CDS             2747..3166
                     /gene="ssb"
                     /locus_tag="ACHA02_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8670999.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3243..3539
                     /locus_tag="ACHA02_002729"
     CDS             3243..3539
                     /locus_tag="ACHA02_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671000.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3554..3985
                     /locus_tag="ACHA02_002730"
     CDS             3554..3985
                     /locus_tag="ACHA02_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8671001.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4290..4472
                     /locus_tag="ACHA02_002731"
     CDS             4290..4472
                     /locus_tag="ACHA02_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671002.1"
                     /translation="MQRTLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKR
                     FTEEIEQLAELNIRYF"
BASE COUNT         1763 a          741 c          999 g         1270 t
ORIGIN      
        1 ttggtatgaa aggcattctc cgacttccag gtttttgacc attttctctc aacgtggaaa
       61 aaccgccact tatgagagaa aatggtcaaa aacctaagcg ggaagtctcc gaataacata
      121 ccaagcgccg acagccaaca gataaagaat acactactaa aaatagtagt agcataaaat
      181 ttaaaaggga aaaaatcaac ccctacccca gtcttggggt ctcaaaacta actcccccct
      241 aaccccctct ttcagcctct tttgaaagag ggggagaatc aaccccacgg ccaacgcccc
      301 agtgttcttg gggctgtcca gaacagaaac ttgcgagaga gtgggggtaa aacaagtgta
      361 gaggaaactt gaagacttgt tcgaacagaa acttgcgaga gagccggagt aaaacaagtg
      421 tagaggaaac ttgaagactt gttcgaacag aaacttgcga gagagttgga gtaaaacaag
      481 tgtagaggaa acttgaagac ttgttcgaac acaaacttac aagagagggg agtaaagcag
      541 ttgcagagga atcaggaaga tttgttcgaa cagaaacttg caagaaagca ttattaaatt
      601 gaaggtgatg aaatatgaag tatttattgc atggctattt tattgattat tctactcgca
      661 atagtagtac tttagcgatt ctatgtttgc ttcatgaatt gagagtggtt actagaaaac
      721 agctatacaa actattgaat tttgacagat caattagtaa acaaggagta acgaatgctt
      781 tagtgagatt atatgaaaaa cagttaatcg atcgaaaaaa atatggaaca gaaatgtatt
      841 attatttgac gaaagaggga catcaaagca taggtgggta ttatacgttg ccgaaagttc
      901 ctgaatataa tttgaatcac catttacaaa ttaacaatta tttgattaaa ggaatggcat
      961 tggcaaaaga ccacccacat ttaaaacgga ttatttcaga aagacgtacc gtctatgaaa
     1021 cgaaggattt aaatcctaag acaaaaggaa gaaaattttt tgtgccagat tttatttttc
     1081 gttttcgaga caaagaaaat catgagatca attggtcctt tgaaattgag ctaacgttga
     1141 aaacacgtcg tcgctaccgt gaagcgattt ttccgaaata tattcgtgag ttgcgaacac
     1201 gtcctgaatc acatttaatt tatgtgacac ctagtggcgc aattcaagaa gaacttcatg
     1261 cgtttaagcg tatttttttc aagcgcgaaa ctgaaaaaga tgcgagtcaa caagaattgt
     1321 ttgagcggtt gcatattttt tcagctttag agtttgaaga gggattaaaa gcattattag
     1381 agaatgatcc agcagtcaat tggcggacag tagaacgaga aaaaatttat ccgaatggcg
     1441 ctgttcaaag tcgtttgaat ttatttgaag aatcaactga aaatgaggaa aaaccaaaag
     1501 tgaagtttac gcctgaagta ttgtaaaaga gggtgttcgc ttcgctcttt ttttgtgcca
     1561 gtttgctggc accgttcaga cgcaagcgtc tgaaccggct cgtttttagg aaggactaac
     1621 ttgtttgatt tatccgttgt caaagaagtg gacgttcgag agtccctaat cgtatagcag
     1681 tcattcggcg agtataacct aaaacataac ctgccaattg ccaagggtaa aatgcacgct
     1741 gcgcgccctt ggcaatcgtc agaacatgtt ttcgagccgg ttatgccgaa cgaccgctat
     1801 acgaaaaggg cctctaaaca gaagtccact ttgatccttg acaactgaaa cccaaaatca
     1861 aacaagcagg ccttcgtcta aaaacgagcc gaatgaagac cttgggggtt gcacccccaa
     1921 acccccgtgc ccccgacaca ctaccgaaaa ggtgggggtg ggtggcgaaa aaatcaaata
     1981 aaacattcta ttcaagaaag gaagaaacaa ccatgacaac taaaaacgag caactgaaag
     2041 aacaggcaag aaaagagttg caagaaagag gtttgattat tgaggggagc tttgaaggag
     2101 attttgaaac ctatattggt tgttatgcaa gaccgataaa caaacctaca gccttagacc
     2161 caatgaacga acaagaagta aaagaacaag aaaaacatgc agtgaatggt tttccgcaag
     2221 atttcactga atggtatgag tgggaaatta acaacgggaa gcttgaaaac ttcttataaa
     2281 agtcagatga cgtgcaaaga gaatgattct ctttgtacgg taaaaatata aagaaagaag
     2341 gaaaaagaaa atggcaaaaa acacacctta tcaagtagga gacatttttt atagtagttg
     2401 gggctacgaa caaacgaacg tgactttttg gcaaattgtg aagttaacag aaaaaacagc
     2461 atggtttcag cctattcaaa agaaaacggt cgagcattat tcgtccatga gtggaaaagt
     2521 tgttccaatg aaagacaagt ttatagagta tcctttagca aaaactaaag acagtattgt
     2581 aaaaaaacga attgatccag aaacctatcc tgatatgtta tgtggttatc gtagttggga
     2641 cacgtttcat agatataaaa atcaagctgt ttatgaaagt agttattatt aaggggatag
     2701 tttccccttt caaaaataat aaatagaaga atggagagaa aaaatcatga tgaattcagt
     2761 gaatttagta ggacggttaa caaaagaagt ggatttaaaa tacacaccaa aaggcaatgc
     2821 gacaggcaca tttattttag ctgttaatcg aaattatacg aatgcaaacg gcgagcgtga
     2881 agcagattat atccgctgtg tcatttggcg gaaagctgcg gaaactttag caaaatatac
     2941 tggaaaagga agtttgattg gcattaatgg acgtcttcaa acgaggagct atcagaatca
     3001 gcaaggacag accatgtatg ttacggaagt tctagtgaca gatttctatt tgttagagag
     3061 taaagaaatc aatgagcaac ggtcaaaatc aatggcgaat aatcaaaata attcttcaat
     3121 tccaggaaat gagattcaaa tttcagatag ttatttgccc ttttaagtga aaaataattt
     3181 tgttctatac ctgatttctt caggtataga acaaaaaata aatagagatg aggcgatttt
     3241 ttatgaaaat cattgacagt tacgatcaac gaaccatgca atatttgggc tcgtttgatt
     3301 atacagatga aaatattatt aattatgttg caagtttacc tccttatcaa agcgttcgct
     3361 tggtagattt tgcaagtgat gaattggtat taacaacgat gggcaacttt ttagattatg
     3421 tcggagacaa aaaatggttg gaaaaaattt tgtctaaatt gattgcgaaa caaacaggag
     3481 aagaacccat aaatgcgatt caattgttca atcgatatga acaagaaaac aatgattaaa
     3541 gaggagagaa aaaatgaaaa gattaaaacg aagtattttt attgtgctag cattgattga
     3601 ttttgttggg acaggttgtg aagcaattga cgttcaaaaa gctcccttat cggaaataaa
     3661 acaagcaata acaaaacaag atgaagaaca accgctaaat acacaagtag aaatagtgaa
     3721 tcaaaataaa ccgagttttt ctgaagaaga tttttcgtta gaaaatggaa ccaggcaatt
     3781 tttttcagat ttggatcgtt taaatcgggt aggtgtagca agtgcacttt tgcataaaag
     3841 caggatgcca acagaagaac gagaccgatt atacattaag ccaactggct ggaaacaaaa
     3901 gaaaatgaag aatggcgatt atctttataa tcgttgtcat ttgattggct atcaacgtgc
     3961 gacaagtttc cccataagta tgtaatacga aacgggagga ttaatcttaa gattaatcac
     4021 aactaaccta tttgacagtt ggaatgaaag ctatcatgtc tgctgaatca actggtctga
     4081 tactcctaca tgcaataaag tctactagga agttattgta tgaagctagg taaaggcggc
     4141 agaacaattt ctaagtgtgg aatcatatga agttggatat gccgggacgc tataaattac
     4201 tcagggtgag actgttttta attaaactga cgaacttgcg aatgtacggg tctataaccg
     4261 acttatattc gaaagatata agattgcgaa tgcaaagaac attatcgaaa agtaagaatg
     4321 ttggtcatga aattcgagat ggaagactag cggtgtatct gtcaagagtt ggtaaaacat
     4381 gtttgattga ttcagatgag agtgggaatg cgacaaaacg ttttacagaa gaaatagagc
     4441 aactagcaga actgaatata cgatattttt aaatacttta gcggagaata aaaaaatcct
     4501 ctacagaaaa ctacggaggg caacatgtgc tattgtttca acaatacatt tttaattttt
     4561 accataacaa aaaagtactt gaaaaaaaac agaaatatgt taaattaaaa atgaaaaaat
     4621 ggaagcggtt acttattctt ttgaatctaa gataaaaatt caaaagaata acataaccca
     4681 aatatgatta tacttgagat tctaaatatt tatcgaaaag gaatttgttt taaggaaaaa
     4741 tgattctatg gacggttatt gatttatatt tac
//
LOCUS       DAYYDS010000141         4712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000141 DAYYDS010000000
VERSION     DAYYDS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.147"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..357
                     /locus_tag="ACHA02_002732"
                     /pseudo
     CDS             106..357
                     /locus_tag="ACHA02_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="ACHA02_002733"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="ACHA02_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8671003.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="ACHA02_002734"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="ACHA02_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1786..1869
                     /locus_tag="ACHA02_002735"
     CDS             1786..1869
                     /locus_tag="ACHA02_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFM8671004.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACHA02_002736"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACHA02_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8671005.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="ACHA02_002737"
     CDS             complement(2994..3857)
                     /locus_tag="ACHA02_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8671006.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="ACHA02_002738"
     CDS             complement(3859..4131)
                     /locus_tag="ACHA02_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8671007.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="ACHA02_002739"
     CDS             complement(4148..4363)
                     /locus_tag="ACHA02_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8671008.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4712)
                     /locus_tag="ACHA02_002740"
     CDS             complement(4455..>4712)
                     /locus_tag="ACHA02_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFM8671009.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1605 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc at
//
LOCUS       DAYYDS010000142         4645 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000142 DAYYDS010000000
VERSION     DAYYDS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.148"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..717)
                     /locus_tag="ACHA02_002741"
     CDS             complement(133..717)
                     /locus_tag="ACHA02_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8671010.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="ACHA02_002742"
     CDS             complement(928..1131)
                     /locus_tag="ACHA02_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671011.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="ACHA02_002743"
     CDS             complement(1141..1563)
                     /locus_tag="ACHA02_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671012.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="ACHA02_002744"
     CDS             1801..2415
                     /locus_tag="ACHA02_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8671013.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="ACHA02_002745"
     CDS             complement(2580..3404)
                     /locus_tag="ACHA02_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671014.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="ACHA02_002746"
     CDS             complement(3428..3583)
                     /locus_tag="ACHA02_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671015.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="ACHA02_002747"
     CDS             complement(4042..4299)
                     /locus_tag="ACHA02_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8671016.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="ACHA02_002748"
     CDS             complement(4292..4561)
                     /locus_tag="ACHA02_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8671017.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1670 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tctta
//
LOCUS       DAYYDS010000143         4609 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000143 DAYYDS010000000
VERSION     DAYYDS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.149"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..398)
                     /locus_tag="ACHA02_002749"
     CDS             complement(84..398)
                     /locus_tag="ACHA02_002749"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8671018.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(449..703)
                     /locus_tag="ACHA02_002750"
     CDS             complement(449..703)
                     /locus_tag="ACHA02_002750"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8671019.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(896..1171)
                     /gene="prgO"
                     /locus_tag="ACHA02_002751"
     CDS             complement(896..1171)
                     /gene="prgO"
                     /locus_tag="ACHA02_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8671020.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1143..2096)
                     /locus_tag="ACHA02_002752"
     CDS             complement(1143..2096)
                     /locus_tag="ACHA02_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8671021.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2708..4201
                     /locus_tag="ACHA02_002753"
     CDS             2708..4201
                     /locus_tag="ACHA02_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8671022.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4336..>4609
                     /locus_tag="ACHA02_002754"
     CDS             4336..>4609
                     /locus_tag="ACHA02_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8671023.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1541 t
ORIGIN      
        1 gcttgaattc ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta
       61 tgttttcttc tagtctacac accttaattt ttctgtctag ttaattgtag cctatccatg
      121 atcggtttat tagacctttg aggtctatat tttttaacta caatcgacct taaatttaat
      181 tgtttcatta gcttttggac atgtttaaga gatactgaaa tcccttcttt taataaaact
      241 tgatggatct ttgtggcacc atagcgttgt ttattcgtaa aaaaaattgt tgaaatcttc
      301 tttttcaact tattatttct gatttctgta gctgtcaacg gtttattctt ataaaaataa
      361 tagacacttc tagggatttc taataaccga cacaacaaag aaacagtgta atccttacac
      421 catttttcaa taaattgaac aagcgaattt aggttttccg agagaatatg gctgcggctt
      481 tttttaagat ttcaagttcc tcatttaaac gagcattttc ttttctcagt tcagctactt
      541 cttttccagt cagtcctgtg gactgatcag gtaaatataa attcttccat ttgtaaatcg
      601 ttgcttcaga taagccatat tctttcgcta accctcgaac agaacgtcca gtttgattca
      661 gttcaacaat catttgttta aaattttctt catatcgggt cataaaaatt catcctctcg
      721 tagataagtt taatttattc ttgtctctac gaaaagctgt ctaagatttg atactaacac
      781 caaatgttgt atacccccaa acaatacaca tattttgaag gattaggatt aataaagcta
      841 aaaatatagt aagtatacta aatatattta ctatatctag tatacttact atattttaat
      901 cttttgatcg tctattatat ataatccgtt cctcgtcgtt taaagtatca acataggcgt
      961 ccattgcaag tttgactatt tcatacatag gaatatcttt tgcataacta agatcccgaa
     1021 tggtttttag tacgggagga tctacttgaa ctggttttct ttctttcgca gtgtactttc
     1081 gttcccctaa ctcgacatta atatcatctt taattgaaat atcttcagca ccaattttct
     1141 tcttatcttt gttcgttggt aataatgcca tttagtttaa tctcctttcc aagaggtagg
     1201 atatgattgg ccattgagtc agcatattta ggtgtataat caaatccttc aatatcacca
     1261 tgttgttcat agtattcaat tctttcttct aattctgtaa agatgtctgc aaaaatagcc
     1321 caccatcttc gatctacata atcatttaat tggataccat tctcaccata catttctaac
     1381 cgatccgagc ctttaacaat gcttttaaaa atgtttgatt caccatactt atcaattgtt
     1441 ttttgatagt ttgcgtgttg agaggaacgt cgcactgaga aaagaacagg caaaattcca
     1501 atgatttgaa gatgagactc ttcaaaattc gtgacaatcg gaactaaaac tttattaata
     1561 aaatcttctg ttccttccat tgcgtaccgt tttaattctt gcattgcaat aatataatct
     1621 gcagcagtta agaatgcacg aaccacatta tctgttgatg gtgggcagtc gataaagata
     1681 taatcgaaat tttttctcaa ttcttctaaa ggtttaatga aggccaatga tctcttacga
     1741 cgatcaggat atttttcgga caagtattca ttaagttctt cagatccagg agagccagcc
     1801 ataatatata aatgtggact tacttgagta atacctgaaa cccagtcccc ttttttgatt
     1861 gctgcagaca tggatcgatc aaagctagta atttgatacg ttgatccaat gttggaagta
     1921 gtatttcttt gttggtcata atcaattagc aacacgtctt tttcaaaaag ttgactagaa
     1981 acaatcgcta ataaatcatt gtctgtagtc ttaccaatgc cgccttttct acctgtaagc
     2041 attacaactt ttcctagttt atctgatgtt ttaaatgcag tccgtaagtt ttccatagtt
     2101 ccctccaatt tcctatacta aatatattta gtatacttca tatacttaat atattcgata
     2161 tatctaatat actccaaatt gataagaatt cagactttat aaggaatata gtggatatac
     2221 taaatatatt tgctatattc agtatatcag atatattcaa aatgtcaaaa tatatttgtg
     2281 cgttttgcgt tgacaattta gagtgaaaag aggtaaactt aattctatag aaaccacaga
     2341 taattcacta tttacaaagt agcaaaagca gtggtactct ttaaacagaa aaattacata
     2401 aaaaaaagag acggaaataa ttgattttag cagaatcaat tatttctgaa tcacatggtt
     2461 aggacatgtg ttcacgctcg tctcaagaat atgtaagtag ctaccaacta cttatattaa
     2521 aagttgatcg gatacctttt ccgattgcct ttattctacc aaaaaaaggc gagcaactca
     2581 acagaagaac gtttatttag tgtacttaaa ttaaatattc ttaaacactg aagagagaac
     2641 acatactatg gctgagatta aagctaaact gtgttctctt tttgtttgag aaaggacgtt
     2701 acaatcgatg ttgagcgaaa tttattcagc tattcttaaa aacggcttac gtacatacaa
     2761 atatcgaaac agcaccatga aacctgcagg tcatattgat tccagtagga gtggtgctat
     2821 ctttgcgtac cgtagcaagc aattaatgaa tgttgggcgt ggtatggtca ttacttcgga
     2881 agaagctatt ttagagaacg aaaagaaact aacgcattgg acacctaata cctatcgctt
     2941 tggtacttat gcggatgatt atcgaacagt ggttaaaggt cattctgaga aaaacttaag
     3001 tcaaatcaac acatttgtag ttgatatcga ttcaaaagag aaccatcaag gcgaaattat
     3061 tttagcctgt ttagatcaag tgggctacat gccaacgcta attttagaat cagatcatgg
     3121 ttatcaagtt tattttgtac taaagacacc aggctatgtc acaaagaaaa gcaatttcaa
     3181 ggtgatcgat gtagcaaaaa aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt
     3241 gtccggagtg gatctaggat gtaaccactt cggaattgct cgattcccac aagaaaataa
     3301 tattgttttt aaagaaatga actatcagta ttcttttgct gactggattc aatggtcgat
     3361 gaagcttgaa tcagatcaaa aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc
     3421 tgagaaaaaa gaaattcgtc aagtagatga accgtggttc gatatgttac ttcataaagc
     3481 aaacatcgtc ggcggagaag gacgtcttgg tcgaaataat gtgattttca cgctttcttt
     3541 agcttattac tcttcaggtt atgcattgga tacttgcgaa tacaatatgt tcacattcaa
     3601 cgaacggtta caggaaccgc tatctgaagc agaattagta aagattgtac gtagtgctta
     3661 ttctggtaat tatcaagcag caagcagaga attcattatt gaattgtgcc agcaatgggt
     3721 ttcaccaacc ttaaaaaata atgatctgtt cattcaacga cgtggctggt ggaaatttaa
     3781 aaagccacga gatcaaagaa aatatagtca taaaaatgaa tggaaagaag atctattggc
     3841 ttatctaagc gaaaaaagtc ttgctagtga gccttatttg aacctgtcaa aaaaagaatt
     3901 ggcttcacaa ctaacgatgc ccaagcgaac acttgataag atcttaaatg agttacgaga
     3961 ggagaataaa gtcttctaca aggtaacacg aggaagaaat ggcggaatga tcattgcttc
     4021 agtcaaaacg ttgttcgcga gggttattca actaagtaaa gaaaagagac tagattattc
     4081 aacaaggatc acagaggtat ttggactcaa cgagagctta gtagaagaga catttagaca
     4141 attatcaacc cccttgaacc ctcttaaaga agtgaatctt tttgaagtag atacaggttg
     4201 aaaaataaaa ggtgcaaatt gccttataca atatatctat gatacttctg taggtgttgc
     4261 tccctttgcc tagctacgtg taggtggtat acttataact gcgtatgcag tcttatgtat
     4321 catggaggaa gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt taatgtgttt
     4381 atcattggtc gtttgggatt atctgtggaa gtgttctgtg agctttatgg ttttaagcag
     4441 gggactttgt cttcttgggt taccagggaa aaaacagttg cttctttgcc gattgagttt
     4501 cttcatgggt tgtccttagc ttctggggag actatggatc aggtatatga ttgtttgtgt
     4561 gtgttagagc aggagtatat tgagtataaa atagctaacg agttaagaa
//
LOCUS       DAYYDS010000144         4473 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000144 DAYYDS010000000
VERSION     DAYYDS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.150"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            324..1052
                     /locus_tag="ACHA02_002755"
     CDS             324..1052
                     /locus_tag="ACHA02_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8671024.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1197..3224
                     /locus_tag="ACHA02_002756"
     CDS             1197..3224
                     /locus_tag="ACHA02_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8671025.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3332..4429
                     /locus_tag="ACHA02_002757"
     CDS             3332..4429
                     /locus_tag="ACHA02_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8671026.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1572 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaatataat atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc
       61 aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct
      121 gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga
      181 taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca
      241 taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa
      301 ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt
      361 tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat
      421 ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg
      481 atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa
      541 caggaggggc attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt
      601 ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac
      661 cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct
      721 ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat
      781 ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt
      841 ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa
      901 caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg
      961 tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag
     1021 tttggacatt agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa
     1081 tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat
     1141 ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga
     1201 aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca
     1261 tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca
     1321 aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg
     1381 atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca
     1441 aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta
     1501 tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg
     1561 ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata
     1621 agaagattag tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac
     1681 gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta
     1741 atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg
     1801 gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt
     1861 cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt
     1921 ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag
     1981 ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc
     2041 aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc
     2101 tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt
     2161 tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg
     2221 atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga
     2281 taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga
     2341 ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc
     2401 aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt
     2461 attttattgc tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc
     2521 ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat
     2581 cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata
     2641 aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag
     2701 agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga
     2761 atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca
     2821 aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta
     2881 gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt
     2941 ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc
     3001 ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg
     3061 ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta
     3121 cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg
     3181 aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata
     3241 taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga
     3301 gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc
     3361 aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc
     3421 agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga
     3481 caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc
     3541 ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac
     3601 caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct
     3661 gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag
     3721 tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc
     3781 tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca
     3841 aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact
     3901 gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag
     3961 aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat
     4021 tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg
     4081 attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct
     4141 aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa
     4201 attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa
     4261 agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa
     4321 aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt
     4381 atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa
     4441 caaatagtaa atttttatta ttgtaggatg gtt
//
LOCUS       DAYYDS010000145         4433 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000145 DAYYDS010000000
VERSION     DAYYDS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.151"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACHA02_002758"
     CDS             complement(543..2048)
                     /locus_tag="ACHA02_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8671027.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACHA02_002759"
     CDS             complement(2045..2731)
                     /locus_tag="ACHA02_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8671028.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHA02_002760"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHA02_002760"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8671029.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAYYDS010000146         4370 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000146 DAYYDS010000000
VERSION     DAYYDS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.152"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..1080
                     /locus_tag="ACHA02_002761"
     CDS             157..1080
                     /locus_tag="ACHA02_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8671030.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1086..2339
                     /locus_tag="ACHA02_002762"
     CDS             1086..2339
                     /locus_tag="ACHA02_002762"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8671031.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2473..3819
                     /gene="gor"
                     /locus_tag="ACHA02_002763"
     CDS             2473..3819
                     /gene="gor"
                     /locus_tag="ACHA02_002763"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8671032.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1503 a          690 c          941 g         1236 t
ORIGIN      
        1 ttttgtcaca atctcttttt atatttggac agaatatatc gaactttatt ttgatttttt
       61 gtgaaataat tttcaaaaat ccgactttaa atagaagaag tattggaaag tttttagagc
      121 tatgctacta ttatgaagtt gtaggaggag aaaattatga tcgaactcat aaccacagta
      181 gaaactattg aacaagcaga acagttgctt gctgcaggtg cagatacact atatttcggt
      241 gaagagcaat ttggattgcg tctgcctgct tcatttacac gagaagaaca gcgacaattg
      301 gtagaattag cacacaagta tggtaaaaaa gcgaatattg ctgtaaatgg gatcattcat
      361 cctgaaaaaa tgaaaaatgt acctgaatat ttggcttttt taaaagaaat aaacgtagat
      421 gtgatcacag ttggtgatac aggtattatt tatacgatga aaaaaaatcc tgaattgcgt
      481 ataccgtata tttatgatgc tgaaacatta gtaacgagcg cacgtcaaat caatttctgg
      541 gcgaaaaaag gcgcagttgg agcagtactt gctcgtgaag ttccttttga agagatgaag
      601 gcgatggaag aaaaactcga tattcctgta gaaacccttg tgtatggtgc tacatgcatc
      661 catcaatcca aacgtccact gctacagaat tactataact atacgaaaca agacgaacaa
      721 aaagatcgtg aaagaggtct ttttatctcg gaacctaaaa aagaagagac acattattct
      781 atttatgaag atagtcatgg cacacatatt tttgccagca atgatctgaa tctctcgaat
      841 gaattgaatg aactttatca gcatcattac tgtacatgga aattggacgg attatttact
      901 ccaggcgaaa aattcgtaaa catcactcga ctattcgccg aagcaaaaaa acaaatttta
      961 gaaggtagct gggatcaaga aaaagcggaa aaaatgacta gcgaaatcag taaattgcat
     1021 cctgaaaacc gtgaattaga tacaggattc ttttatattg atccttctgc tatcaaataa
     1081 ggagtatgat gaaagaaatg acagtaaaaa gaaaattgaa acgaccagaa atcctagcgc
     1141 cagcaggaac actggaaaag ttaaaaaccg cgatccatta tggagcagat gcagtatata
     1201 ttggcgggaa tgcatatggt ttaagaagcc gtgcaggtaa ttttacttat gaagaaatgg
     1261 ctgaaggagt tgcttttgca aaagaacatg atgcgaaggt atacgttgct gctaatatgg
     1321 tcacccatga aggaaatgaa gatggagcag gggaattttt ccgcagtttg cgtgatgtcg
     1381 gtatttctgc agtgattgtt tctgatccag ctttgatcga aatttgtgcg acagaagccc
     1441 caggtctgcc gatccattta tccactcaag cttcagcaac taattttgag acattggaat
     1501 tctggaaaga agaagggttg gaacgtgtcg ttttagctcg tgaagtttca atggaggaag
     1561 tggcagagat tcgtaaaaat acggataccg aaatcgaagc atttatccat ggtgccatgt
     1621 gtatctctta ttctggtcga tgcacattgt ccaaccatat gtcgatgcgg gatgcaaacc
     1681 gtggcggatg ttctcaatct tgtcgctgga agtatggatt gttcgatatg ccttttggtc
     1741 aagaacgaaa cagtttaact gatacaggcg aagtggaaga agaattctcg atgagtgcag
     1801 tggatatgtc aatgatcgaa catattccag aattgattga aaacggcgta gacagcttca
     1861 agatcgaagg aagaatgaaa tcgatccatt atgtatctac ggtgtcaaat gtctataaag
     1921 cggctgtaga cagttatatg gaagatcctg aaaactatgt ctgcaagcaa gaatggatcg
     1981 acgagttatg gaaagtagca caacgagaat tagctacagg tttttactac aatacaccaa
     2041 ctgaaaacga acaattattc ggtgaacgtc gtaaaatccc agtatataaa tttgtcggtg
     2101 aagtattatc ttatgatgag gcaacacaga ttgctacgat acgtcagaga aacttgtttt
     2161 ctgtaggaga tgaaatcgaa ttttatggtc caggattcag tcatttcagt caaaccgttg
     2221 aagttatgca taatgaagaa ggcgaatcca ttgaccgagc accaaatcca atgatgattt
     2281 taactatgcc agtcgcacag ccggtgaaac ctggagatat gatccgaaaa cgtaaataag
     2341 gaaaaagctg atatatccct gttttaagca tcttcgcata caaaattcaa gaaaagtgtt
     2401 acacttgttt tctgtagaaa aaggatcgtt aaataaaggt tttgattgat ctcttcaata
     2461 aggaggaaca aaatgcaaat atatgattac atcgtaattg gtggcggctc tggcggtatt
     2521 gcttcagcaa acagagcagg gatgcatggc gcaaaagttt tgctgattga agcgaatgaa
     2581 ttgggcggaa catgtgtcaa cgtcggttgt gtacctaaaa aagttatgtg gcaagcaagt
     2641 acgattttgg aaacaattga acgagatgcc tcaagttacg gtatccaagc agatttaaaa
     2701 aatgttgatt tcgccggtct agtagaaaga cgagaaaaat atattgattt tctacacggt
     2761 gcttatcaaa gaggattaga tagcaatcat gtcgaggcta taaaaggtta cgctactttt
     2821 atcgatagtc aaacgatcga agtaaacggt gaaacctatc gtgctcctaa aatcttgatc
     2881 gctactggag gccgtgcaag taaaatgacc attcctggtg gagaatacgc aattgattca
     2941 aatggatttt ttgaattaac agagttacct aagagagcaa tcgttttagg tgctggatac
     3001 attgcggctg aactttcagg tgtatttcgt ggtttaggta gtgaagtcat gtgggcatat
     3061 cgaaaagaac gaccacttag aacgtttgat aaaatgcttt cggacaattt gatccaaatg
     3121 taccaagaag ctggaataaa aacttacgca catcatattg ctaaagaaat tactaaaaac
     3181 aatgacgaat atacggtgct atttgaaaat ggtgaaacct taactggtga ttgtgtactt
     3241 tttgccggtg gacgtgtacc gaataccgaa aaattaggtc tggaaaatac aaatgtggaa
     3301 ttggacaaaa aagggtttat caaagtagat aaataccaaa atacaacgga tgaacatatt
     3361 tttgctgttg gtgatgtgat cggtaaatta gatctgactc ctgttgcaat cgcagctggt
     3421 agacgtttat cagaacgttt atttaatgga aagaaagatt cttatcttga ctataaactt
     3481 gttccgacag tcgtctttac gcatccacca attgcaacaa ttggtttgac agaagaagaa
     3541 gcattggaaa aatatggcga aaatgaattg aaagtttatc gttcacgctt taccccaatg
     3601 tactttgcat taaatgatta tcgccaaaaa tgcgaaatga aattgatctg tgtcggaaaa
     3661 gaagaaagaa tcgtcggctt acatgctatt ggtgttggtg tcgatgagat gctgcaaggt
     3721 tttgccgtgg ctatcaagat gggtgcaact aaagaagact ttgacaatac agtagccatc
     3781 catccaacag gagcggaaga attcgtaacg atgcgataat ttaatattaa cagttccaac
     3841 agctttttat tgaacaaacc atgtaaaacc agaaaatcag tatactgatt ttctggtttt
     3901 ttgtttttta gttatttatg taagattgct tcgataattc tcaaaacgaa ataagtaggg
     3961 aagttggaga ttttgattat cttcgtaact gacgaagagt aaatgaatcc atttggtatg
     4021 cactcttaag ttcaaaatat tggtatcgat gtttaaaaga aatagaaatg tatcgaagaa
     4081 cagatgttta aaaaagtgtg actttgtata aataaattag aagaagcaaa cgaggatgtt
     4141 aaaagaatac catatagtta ccaaaccaag aaagttctcg tcaagttcgc tggtacgaat
     4201 caaatcaagt gaaaaaaggg actgtgaaaa aaattgtgtc atagttagtc actttttccg
     4261 aacaaatagt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag
     4321 gaactatttt tccatttgtt tctctaatac tcaggacaaa acactgattt
//
LOCUS       DAYYDS010000147         4345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000147 DAYYDS010000000
VERSION     DAYYDS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.153"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1853)
                     /locus_tag="ACHA02_002764"
     CDS             complement(387..1853)
                     /locus_tag="ACHA02_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8671033.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /locus_tag="ACHA02_002765"
     CDS             complement(2144..4228)
                     /locus_tag="ACHA02_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8671034.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DAYYDS010000148         4324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000148 DAYYDS010000000
VERSION     DAYYDS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.154"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACHA02_002766"
     CDS             complement(<1..281)
                     /locus_tag="ACHA02_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8671035.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="ACHA02_002767"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="ACHA02_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="ACHA02_002768"
     CDS             complement(2359..2721)
                     /locus_tag="ACHA02_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671036.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3033)
                     /locus_tag="ACHA02_002769"
     CDS             complement(2725..3033)
                     /locus_tag="ACHA02_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671037.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3042..3461)
                     /locus_tag="ACHA02_002770"
     CDS             complement(3042..3461)
                     /locus_tag="ACHA02_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671038.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="ACHA02_002771"
     CDS             complement(3454..3687)
                     /locus_tag="ACHA02_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671039.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="ACHA02_002772"
     CDS             complement(3684..>4324)
                     /locus_tag="ACHA02_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8671040.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DAYYDS010000149         4322 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000149 DAYYDS010000000
VERSION     DAYYDS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.155"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..573)
                     /gene="raiA"
                     /locus_tag="ACHA02_002773"
     CDS             complement(22..573)
                     /gene="raiA"
                     /locus_tag="ACHA02_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8671041.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(701..1420)
                     /locus_tag="ACHA02_002774"
     CDS             complement(701..1420)
                     /locus_tag="ACHA02_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8671042.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(1417..2730)
                     /locus_tag="ACHA02_002775"
     CDS             complement(1417..2730)
                     /locus_tag="ACHA02_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8671043.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(2811..3131)
                     /locus_tag="ACHA02_002776"
     CDS             complement(2811..3131)
                     /locus_tag="ACHA02_002776"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8671044.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(3239..3526)
                     /locus_tag="ACHA02_002777"
     CDS             complement(3239..3526)
                     /locus_tag="ACHA02_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671045.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            3653..4297
                     /locus_tag="ACHA02_002778"
     CDS             3653..4297
                     /locus_tag="ACHA02_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8671046.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
BASE COUNT         1259 a          891 c          765 g         1407 t
ORIGIN      
        1 ttgttattta agtaaggctg atcagtctgt ttcaatcagt ccgtattttc catctttacg
       61 gcggtaaacg atacttgtac catttgtttc tgcatcttca aaaataaaga agttatgtcc
      121 cagcatgttc atttgtaaga ctgcttcttc actatccatt ggtttcaatg aaaggcgttt
      181 cgtacgtacg atatctaatt cagaatcatt ttcgttttcg tcctcttcat tgaataaagc
      241 agtagcatct gcagtattca tgcccgtttc acgggattta cgattaattt ttgttttgaa
      301 tttgcggatt tggcgttcca atttgtcaac aaccaaatcg atacttgcgt ataaatctgg
      361 agaagtttct tcggcacgta atacaagata aggtaatggg atcgttactt ccacttttgc
      421 tgttttttct gtataaactt ttaagttcac atgcgccgtt gcatcgggtg cgtcactgaa
      481 atagcgttct agtttgccta cttttttctc aacgtagtcg cgaatcgctt cagttacttc
      541 gatattttcg ccgcgtacat tatatctaaa cataaaaatt acccctttcg tctaccacaa
      601 tcaagaagga gaaggaccac tctttccctc ttcttaattc tattatatcg aactctaggt
      661 tatttgcaaa ttaaatcgct atcaaatttc cacatctaat tcatcttgct aaagaaatgg
      721 ttcggatgat tttcggctgt gcgacctgca aacaatctgc tgcgtggaaa agagtctgtc
      781 ctgttgtata gacatcatca ataatcaata tttttttatt ttgtatctta gaaaaggtga
      841 ctgcaagttc aaaaggttga ggcatctcca atctttcctt tcgcgtgttt tttgcttgag
      901 ccagcagatg cttctctctt ttgagtagca tctgaaaagg aattcttgct gctttcaaca
      961 tctcgcttac ttgattgaat cctctttctg ttcgcctttt ttcagataaa ggtattggac
     1021 aatagatgta tgtcgggtat tcttttttta tctgcgtcca tttatatgca aaggtgcctg
     1081 ctagtcggat atctcccata aacttgtatt gattgagcca ggccttcata ttttcatcgt
     1141 acacaaaaaa ggcacggtgc tgaaaatcat aggtcggata tttcttcttc cattcttgac
     1201 agtcactgca gtaaacggga atagacttta ctttcttttt cgtactctct agattgaaat
     1261 ggattggttt ttgacagcca gaacagcctc ttgtttccaa taaatgaaat tgtgcgcgac
     1321 aaactgaaca agtctgaggt aatctgtatt tcgaaacacc aataatctcg ctgaatgtta
     1381 aattttggac gatatcttta ctgcagcaac tgcacctcac agcatcttct tcccttcttc
     1441 attcatttct ttgatttcct gacaagcttt tcgaatagct gcagtttttt cttcatagaa
     1501 aaagtatact tgactgttcg tataaggccc tttacgatcg gctcttcctg cgatttgtac
     1561 caaagaagat ttagtaaaaa ccggatgatc cgctcctaaa acgataacgg atattctttc
     1621 aaatgtgact ccacgttcaa ggatcatcgt ggttaacagt atgtcatatt ttgcttctcg
     1681 cattttctgg actttttccg cccgctgatt gtctcttgaa gaaacatctg tgatctcata
     1741 tctcggaaga gcctcttgaa gtgtggcttt caaacgatgc atcatcgtga tacttggaca
     1801 aaataaaagg atatggtttt ctttaattaa ttgatgaatg atccgcacca aagattttat
     1861 cttcttcttg ttcttgcatt tttgtcgcca attattcatg aaaaccaaga cgggttcagg
     1921 aagtaaacgc cgatgaaagc gcaaagcaag tttgtggatg tcaaacgtct gcttgatttc
     1981 ctttagtaac tttttatcag gtgtggcact taagtagatg aatcgtccgt ctggtttaat
     2041 cgctgtttgc acagcatgag ctaatcctgc atccccgtta taaggaaacg catcgatctc
     2101 atcgatcact attaaatcaa aggaccgata aaaacggtat agctgatgaa tcgtacagat
     2161 aacaaaagaa gtaaaggtat aagccgttgc tgtattccca tggagcaacg tgattttttc
     2221 ttctggaaaa acataacgat agcgtaaaaa caattcatta caaacatcga ctcgcggtgt
     2281 acaaatcgct actcttcctc cagcttttaa agtttccact aaaataggat aaagcatctc
     2341 tgttttccct gcacctgtta cagcccaaat aagatgatgt cttttttctg aatggcaaag
     2401 tattctcttt gcgatttccg tttgtttttc agtaagctgt cctgtccaaa aaaagacgac
     2461 ttctcgaggt tttgcttcag gctgatcaaa caaatataaa tcttgttgag tgtcgcatcg
     2521 ccctaaatat agacaatgtg tacaaaaata accgtaatct gcaagctgtc catcaatgac
     2581 ggacgtcctc attccacagc gatgacaaag aatccagttg cccaaacgtt ccactgctgc
     2641 gatcttctca taatttggta aatttacctc ctcacagtct attgctttaa tatctctcgt
     2701 cagaagcctt ctaccccaaa aatgattcat attattcgcc ctaaaaaaat atacgaaaaa
     2761 acaagcgatc tattttttgg atcgcttgtt cgatagttat actgttctat ttattcacct
     2821 atgagcgtga ttgcttgatc taacactttt tcgccattca tcataccata atcagccata
     2881 ttgatgacgt ctaaaggaat gccttttggt tccagacgtt tttcaaaatc agctttcata
     2941 aagcgaactt gaggtcctaa taacagtaca tctacatctt tatttgcaag attgttatct
     3001 gcgtcagaag cggacacagc gaaaatatcc gagtcgattc cttttgcttc agcagctttt
     3061 tgcatttttg ttactagtaa acttgtgctc ataccagccg aacatacaag catgattgtt
     3121 tttttagcca tacttttctc tcctttttaa aacaaactac gcaaacgttt tcttcgcgtc
     3181 tgttactcta ttatataaaa agaaagaaat ttgtcaacgt tttctaaacg cggtttattt
     3241 aatcaaatag ccaactagat ttcactaatt tattgaacac gacatattct ttgtccaaat
     3301 tgatgacaac tacttcatag cctgcattta tccagccata ggcacctttt tcagggtgtt
     3361 tataatcatt tgcccaccaa tctgtacttt cccttgctgt tttgggaagt ccgttactgg
     3421 gaaacagcag ttcgtcaaat tgggtaaagg tcaacgttac ttgagagccg ccattgttct
     3481 tcaaataatg cgtaattgca tcgtactttt tcattcgttc ctgcatcccc agatccacct
     3541 acctttgtct ttattatcgc atactacagc gattgcgcaa ccgttacagt gttgaaacaa
     3601 cctagaactt tatttatact gaataaagaa aatgaaaaaa agaggtgaca agatgatcga
     3661 gcgctatcgt acaataaaag aaaatggaca aagtgagatt gagatcaaaa aatcacgctt
     3721 tatctgttct cttagacgaa tagaatcaga ggaccaagca aaaagtttca ttcaagaaat
     3781 aaaaaaagaa cattggaaag cgaaccataa ctgcagcgcc ttcatcattg gagaaaaaaa
     3841 tgaaatccag cgaagcagcg atgatggaga accaagtgga acggctggcg taccgatgtt
     3901 ggaagtcctc aaaaaaaatc aactgatcaa tgtagctgca gtagtcactc gatattttgg
     3961 aggaacgaaa ctcggcacag gcggattaat acgagcatac actcatgctg tctctcaagc
     4021 attgtctgac attggaatcg tagaaggtac cttgcaacag gagatctctg tgatgatcac
     4081 gtatccgcaa ctaggaaaat tacagaatta tcttgaactc caacattttt ttataaaaga
     4141 aatccaatat actgatcaag ttagcgtcat ctgtgctgta gatgaaaaaa gtgctgacca
     4201 ctttaaagaa tccatcattg agctgctgaa cggacaagtc caatttgaag agggtcctgt
     4261 ttcttatcac gagcaactgg taactgatca tatataaaaa caggtgattc tgattctctt
     4321 tt
//
LOCUS       DAYYDS010000150         4310 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000150 DAYYDS010000000
VERSION     DAYYDS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.156"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..1285)
                     /locus_tag="ACHA02_002779"
     CDS             complement(65..1285)
                     /locus_tag="ACHA02_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8671047.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="ACHA02_002780"
     CDS             complement(1305..1916)
                     /locus_tag="ACHA02_002780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8671048.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="ACHA02_002781"
     CDS             complement(1959..2891)
                     /locus_tag="ACHA02_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8671049.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="ACHA02_002782"
     CDS             complement(3312..4172)
                     /locus_tag="ACHA02_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8671050.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DAYYDS010000151         4216 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000151 DAYYDS010000000
VERSION     DAYYDS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.157"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..1353
                     /locus_tag="ACHA02_002783"
     CDS             52..1353
                     /locus_tag="ACHA02_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8671051.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1380..1640
                     /locus_tag="ACHA02_002784"
     CDS             1380..1640
                     /locus_tag="ACHA02_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8671052.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2639..3874)
                     /locus_tag="ACHA02_002785"
     CDS             complement(2639..3874)
                     /locus_tag="ACHA02_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8671053.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1221 a          674 c          876 g         1445 t
ORIGIN      
        1 gcgttgtcat gcaatgaatg gttcatattt tattgaaaag gagtggtttt agtggaaaag
       61 ctgatgaaat gggtcgaaga gaaacttgtc ccaccaatgg caaaaattgg tacacagcgt
      121 catttattag caattcgtaa tggggttgtt tcaacattgt cattgatttt gattgggaca
      181 ttttttatgg tctttatcaa tttaccgttt cctggatgga atgaatttat tgccccttat
      241 tcggcaacga ttgttttacc ctttaggatg acgatgggat tgatggctat ttatgcaact
      301 tttgttatgg gttcggatct agctaaatca tatggattag atagtgtgac aggtggtata
      361 ttatctttag gaacgttctt tatgttacaa gttcctgtaa acgttttaac tcctgaagag
      421 gcacctcttg gctgggtctt acctatgagt tctttgggag catctgggat gtttgcgggg
      481 atcttatcta tgatttttgc agtggaagta tatcgtttct ttaaaaatcg aaatattact
      541 atcaaaatgc cagaacaagt gccacctgct gttgcccgta gttttgaggc attgattcca
      601 ggtgcagtca ttttaacaat tacttggctg attcgaagcg tcgcgggctt tgatgtaaac
      661 gctgccttat tatcgttatt cgagccttta accaatattt tagggaataa tttgcttggt
      721 gttttactac caatgttttt gatccatttg ttgtggtcat ttggtatcca tggcatgagt
      781 attgtagggg ctgttgttcg tccaatgtgg ctgatcatgt tggatgagaa tgcaaaggca
      841 ttggcagatg gtacagctgc cacccaatta ccctatatcg ctcctgaaca gttttatcaa
      901 tggacggtaa caattggtgg tgcaggagca acgatcgtgg tatctgtttt gtttatattc
      961 ttctgtaaat caaaattctt gaaagaggtt ggtcgttttt ctattattcc tggtatcttc
     1021 aatatcaatg agcccataat ttttggagct cctatgatgt tgaatcctta catgttcatc
     1081 ccgttcaact tagttccttt agttttgact attatcagtt attttgcagt caaattagaa
     1141 atggtcaata gatttactgt tttgccaaca tggacattac cagctccaat cggcggttat
     1201 ctttcagcag gaaatgactg gcgtgttgtt atcttgatca tgatcaacac attgatagca
     1261 tttattatct attatccatt tgtaaaagct tatgataaaa agatgctgtc agatgaacaa
     1321 agtcgtggag aaaacgaagt ccagactgtc taagtttggt ttttagaagg tgggaaacaa
     1381 tgtattggct gattttattt tttgttttta tctttttatt aacggctagt caccttatat
     1441 tgaatatgct agctacatat catattcaaa tcaatcgatg gatttgggca ctggcatcat
     1501 ttttgatcgt gattcttcca aagatcattg taccgcatat gaatgttttg ttttcatggg
     1561 ggacatatgt actttgtgga atttttgcta tcaattttat gatcgagcag catagatggt
     1621 ttgtcacatc gaagctttaa atgttctaaa agtggttgta acagaagtgg ttagcttcaa
     1681 gaaataagaa gaaatttacg aaaattgctt cttaaatttt tgtgaaatgt tggcttattt
     1741 ttcgtttggt tgaacttatt accgcagatg caagcttttg accaccgttt attcggaata
     1801 tgggactgtg acaaggcttt tgtcacagcc cttttttaca taaagattat tattgtttta
     1861 actgttttgg cagaaagaag gaggaaaaaa tggaaatttt atcagcaatg gatttagaat
     1921 tagtagatct actaccaaag tgatcaagtc aaatatagat ttgggagctt acaaccatat
     1981 agtaggggct gcagtatggt gtatgaatgg taatatgtat gatgtgagtg tttattcaaa
     2041 tcatgaaacg tgtatagaaa taattgtgct gggaatagca atcagtcatg gtgaacgtga
     2101 cttttagtgt atcgttgctg acgacataga acattctgac atgatctact caatatgtgg
     2161 ggattgctgg cagtttatat aggagtatgc atcggattgc gagatcattg tctcaacaga
     2221 agagggaggt tgattatcaa agataaagtg gtaaatccta catttgattt agtaattgtt
     2281 gaggggacta gcggcacaaa tctctacaaa gcgatgcttc tagatctata tgacacattt
     2341 taagaaaatg tatttgccta attattatgg tctttctttt gaagaaacgt tgagatgatc
     2401 caaccagaga aaggtgtcaa agaatttgac taagaataaa tggcagacgc tacctatgct
     2461 catttggata gcgtcagatc ttttatttta aggatgattt tgtgtttttt agacaccgta
     2521 atttaagtaa ctctaacttt tcaatccact atttactttt tttgtatttt cactgtctgg
     2581 gtctcgaata gcgaaattac tgatgagtct ctattaaaaa agctttttct acttgaaact
     2641 atattgggtg gttgctcagg aaggtttcga tttcttgaat tgcttgttgt ggaccattta
     2701 ttggaaaaac ggcttgcagc gtgcgtgcct gctgacgaat cttgggtgaa gaaacttcgc
     2761 gcagagcaat ctgcagtttt ctttcagtca ctggttttgt gagggcgatt gcgtttccgt
     2821 attttacaca atagtcaata ttacagcttt gctcaaattg catccccaca ccgataaacg
     2881 gtttgccgga ttcacaggca gtttggacgg tcccttcccc gccgtggata aaagaaaaat
     2941 caatgaattc atgcaaaaga tgactagaca gatattctgt tattaaaaga tcttctgagt
     3001 cagattgaat gtcatcaagc agatagtcaa taggtgcaat aaccgctaga tccggttgtt
     3061 tttgtaagaa gaacaaaatt ttgcgtaaca tacgcggatt gcctgaacta cccattgcga
     3121 aatagataat acgttttctt tcttgttgct ttcgctgtat aaaagttagc acttcttcag
     3181 gcaaggaagt actgagctta gcaaaaattg gaccaacgat tcgataattt tccggcagct
     3241 gttcttcttc caaaaatagt ttaggcgttg tgatgagatt tagatcagca tccatcattt
     3301 gtaaacttgt ttttggtaaa ggaagcattt ctttttgtgc agcgtgtttg aaggcattgg
     3361 ggatccatcg ggtatttact atacgctttt ttgtccagtg ccaaagggga ttgcttaaag
     3421 ggccgaagaa acgtaactcg tggggcatca gcgcatgctc tgattgaaaa aaaggttcac
     3481 tcaaagcata gggtttaaca tatactaaag gaatcttttt attgcgggcc gataaaaaaa
     3541 ttgtcacatt cgaacctgtc acaatcagtc gaggctcaaa cttagcaatc agactctgtt
     3601 cactagtgat acgagaggca accatttctt tggtaaaagg gtttttaagt gtacgcaact
     3661 ggtcaaattt tatgatttgt tcttcttgtt ttttcgtaag gatcggcgct agtaaatgat
     3721 actcaaagcc actttctgtg atcaaaggag caaaagtttt agaaaagcca aagaatacac
     3781 agtaataacg gtctttcata gctttggcaa cctcaatcat tcgtgttgtc tcagctaaat
     3841 tgaatactgc aggagcaaaa agaattgtct tcatgaaatc ctccttattg gaatgagtct
     3901 atcccttcgt ttcttactag tatactaaaa aacacagcgt tcgagtagcc tatgtatatt
     3961 ttgaatatta aactgtctct cgctgttttc acaacttcta aatttaaatt ttgtaatcaa
     4021 gttgaaactc caattttcaa aaaaacacgc aacaattttt tgatcgattt atatgttgtg
     4081 ccagataaat acgagaagtt gtgtcattaa aaataaacat gagaacataa aaaaataaaa
     4141 taattatatt aattgatagg tgtgctgtcg tcattataca cacgtaagaa agaaatagtt
     4201 atgtttaaaa atattc
//
LOCUS       DAYYDS010000152         4069 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000152 DAYYDS010000000
VERSION     DAYYDS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.158"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..106
                     /locus_tag="ACHA02_002786"
     CDS             <1..106
                     /locus_tag="ACHA02_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8671054.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="ACHA02_002787"
     CDS             complement(359..508)
                     /locus_tag="ACHA02_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671055.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="ACHA02_002788"
     CDS             970..1182
                     /locus_tag="ACHA02_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671056.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="ACHA02_002789"
     CDS             complement(1460..3259)
                     /locus_tag="ACHA02_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8671057.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="ACHA02_002790"
     CDS             complement(3383..3979)
                     /locus_tag="ACHA02_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8671058.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1456 a          717 c          700 g         1196 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaa
//
LOCUS       DAYYDS010000153         4026 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000153 DAYYDS010000000
VERSION     DAYYDS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.159"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..593)
                     /locus_tag="ACHA02_002791"
     CDS             complement(51..593)
                     /locus_tag="ACHA02_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8671059.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            728..1117
                     /locus_tag="ACHA02_002792"
     CDS             728..1117
                     /locus_tag="ACHA02_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671060.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1121..1885
                     /locus_tag="ACHA02_002793"
     CDS             1121..1885
                     /locus_tag="ACHA02_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8671061.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2014..2961
                     /locus_tag="ACHA02_002794"
     CDS             2014..2961
                     /locus_tag="ACHA02_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8671062.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3091..3831
                     /locus_tag="ACHA02_002795"
     CDS             3091..3831
                     /locus_tag="ACHA02_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671063.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1338 a          686 c          814 g         1188 t
ORIGIN      
        1 ctaacattta aaatttaatt tagatattag acagttttta agtttgtgag ttattttcct
       61 tgtcgttttc tcactttttt agtccagaaa ccacctgaaa tatattttgg ctcataagaa
      121 atgataaaag cacgctcttc gatttctttg atcaggcgat acagttcacg ttcgcttttt
      181 ctaggagaaa gtatttcgag aactagccgc tcaccttcca gccccattgc agaagtctgt
      241 gtcacaccat aaccgtgttc tctcagtata cccggcaaac ttttatcttc tgcgctattt
      301 gctggcaaaa tcgttgtgac catgatgtat cctaatgcca atttgtcttc aatcaaaatt
      361 ccgacactga tacctactgc gtaacctaac gcatagacaa ataaattcag tggattatct
      421 aaacgatcta aaacaaggga taagccgacg atataaatcg tgatctcgac catactgaca
      481 agtggagcaa tcagtcgata gcctttcatt gtcaacatga aacgtaacgt gttcagagtg
      541 atataagcaa aattaatgag gaagatcatc cccaacattt ttggatcaat catttgtaaa
      601 actcctttca tttatataac aacaatatca aaaaaaatcg taagtgaaga aaaattgaaa
      661 ctttatacat acaaaaaaat gataaaatga ttataataga attataaaga taggttaggt
      721 gattaaaatg aaaacggtca atcgtgtgtt ttgtgccagc cacgtgctaa gtctccttct
      781 cagtatatac ttaggtgtct attcgattca acagccaatt gaggtagaga acattgtttt
      841 tcagatgtcg ctgacagatg gtttaatttt aagcgttttt ttctttttga tactgtttat
      901 tggtaatatt ttcggtagcg ttgccagttt tttgaatttt tctatttatc cactcctttc
      961 gctagcatta ggagtggtgg gactttgttc tctgtgctta tttcccgagc cattcagtcc
     1021 tgcatttata ttatttggta cactgaacct gttccagaca actgttggag catggttgct
     1081 atggcgatca ggaaatttaa tgaaaatagg tgagtaacaa ttgaatccaa ttatattgaa
     1141 acgttctgtc catcatattc cagtgttaga agttgttctg aaagaattaa gagaggaacg
     1201 tattcccgtt gtcatctatt atcacggatg gcagacaagt aaggagttag ttttgacgca
     1261 aggaagaaaa attgcgcagc agggcattcg tgtgatcctg ccagatgcga tgaatcatgg
     1321 ggagcgcaaa cagccggttt cttctattcc atcctataca ttttgggata gtatctacgg
     1381 aaatctgttt gaatttgaca cacttatcaa gcatttagaa aaaagaggtc ttctacatga
     1441 tcagcttgca gttggcgggg tttcgatggg gggaatgaca acatgcgcct tgttggctaa
     1501 acatcctcat atagtagcgg gcgtgtgttt gatgggttcc cctgcacctt tggaatatgg
     1561 aaaagaaatc gtaaggcgcg catcggaata taattatgtc gtaccaaaag actattttga
     1621 tttgatttca tgggttaaga gctatgattt gtcattgcag ccggaaaagt tagctggacg
     1681 tcctctgttc ttttggcatg gacaagctga cgaaaaaatc ccttatatac aaacggaacg
     1741 atttgtagaa aaaaatcggc ataagtctta cggaaaagaa attgtttttc gttcctcaaa
     1801 aacacagcga catatggtac aaataccact aatggaagaa gcttccactt ttttagcaaa
     1861 aaatttgata aaaaagtata aataattgaa acggaaaatc actcatgacg atagaggatt
     1921 ttccgttttt tattttgggc tataataaac tctgttagga aaatttggta taattgatag
     1981 taagaatgac aacatgttag aaaggaagag agcatgccca ctttagcaga tgttgcaaaa
     2041 cgtgccaatg tttcaaaaat gacggtctct cgagtcatca atcatccgga acaggtgact
     2101 gacgaattga aagagttagt tttctcagca atggctgaac tggattaccg tccaaatatt
     2161 gctgcaaaag cacttgtcac gaatcggtcg cagatcatca aattatttat actggaagaa
     2221 atcgacacaa ctgagcccta ttacatgaat ttgctgatgg gaatcgctaa acggatcggt
     2281 aaagcgcatt attctctcca attagttaca aatgatgcct tcgacgtcgg ttcttgtgat
     2341 ggatacatca tcacaggtgt cagaaaaaaa gacttcgatt ggatcgaccg tttggaaaaa
     2401 ccagttgtct tgtttgggga aaattctgaa ggatatgatt atgtagacag cgataatgaa
     2461 tatggtacag caaaagcaac gcaatatgcc ttagaaagag attatcaaac gattatttat
     2521 atcggtatcg aagtggacga gccttttgaa atttcaaggg aaactggtta tttatctgtg
     2581 atgaaggaag ttgatcttcc tagtaaaatc ttgcggtttc caaatcattc gacagaagta
     2641 gaaagatata tgacagaaaa ctgggcagat tttacagata aaacttgttt tgtatgtagt
     2701 tcagatcggc tagcactagg agcagtaaga gggattataa atgctggagg acacttgccc
     2761 gaagaatttg gtgtgattgg atttgatggg gtatttttag accaaatcag ttcacctaaa
     2821 ttgacaacga tcaagcaaga tattatacgt atgggtgaag tatgtggaga aatgttgtta
     2881 aaaaaaatag aagaaaaagg ggcgagtcaa ggttatcgtc gtttttttcc tgaactagtt
     2941 atcagagaga caacgaatta agtcttatta ctatatatat atttatgaaa ttcatcgcta
     3001 ttttcttaaa ataatcagcg cattgctact gaaaacaatg aatctgtgct attattgaga
     3061 aaagttccat ttttgagaag gatggtttga atgaaagaaa tcagacgaaa acatttccgt
     3121 tttcctgcgt acgatgatga gttaggcgta aaattaacca acgacaagaa gcgagtatta
     3181 tttgacggac aagaagatct tttgactgaa cagaatcagt ccccgtttga acagatgaaa
     3241 gaatttcatt tcacgaacac gaacaccgag aaaatccgtc gcaagcgtcg tgcaacaaat
     3301 ggattgaagt cagctccgcg taaggaaaat ttggaaaagc ataaaggaaa attaccggat
     3361 tataccggcc attaccaatc agaaatcaca tcgacaggac gaaaaaaact ttttggtgat
     3421 tcctctcgtt ctgtcagaca aagtgaaaca aggacagagt ccaagcatac tacacgaaac
     3481 gcatctgttg acacgaaggt gcgggagcgt tcttattttg ttcctaaata tgtacccgca
     3541 tcgattatcc cagaccctag agaaagcaaa atttctgagg atcatttaat cgaatcgatg
     3601 aaaaaaacgg atgactctta tctgttgttc gatacagaac cgacgccgta tcaagaaaaa
     3661 agaaatggtg agccgtctgt acgaacattc aatcgaacaa aaacgttaga ggaaacaaag
     3721 cagtcagaag aaaaaaaatc gaaaaaaagc catggtatct tagaacgctc attacaagga
     3781 ttgatcgatg atcaaggaaa ctctttggga gaaaatagct attttaaata agtgatatac
     3841 aaatttatat tcggctacag ttggaaagga aacgctgcgg cgtttcctta ttttatactc
     3901 tatccgaaag tttatatcgt atcctcaaat ttttttgagt ttaagcgaat tttttctccc
     3961 aataagtaag aaaataatgg caaaataaaa aaacaaacta ttttcgcaga tcgtttgcaa
     4021 aaatag
//
LOCUS       DAYYDS010000154         3980 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000154 DAYYDS010000000
VERSION     DAYYDS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.160"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..715
                     /locus_tag="ACHA02_002796"
     CDS             56..715
                     /locus_tag="ACHA02_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8671064.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            800..1705
                     /locus_tag="ACHA02_002797"
     CDS             800..1705
                     /locus_tag="ACHA02_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8671065.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1707..2339)
                     /locus_tag="ACHA02_002798"
     CDS             complement(1707..2339)
                     /locus_tag="ACHA02_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8671066.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    2490..2615
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2659..3183
                     /gene="infC"
                     /locus_tag="ACHA02_002799"
     CDS             2659..3183
                     /gene="infC"
                     /locus_tag="ACHA02_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8671067.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3255..3455
                     /gene="rpmI"
                     /locus_tag="ACHA02_002800"
     CDS             3255..3455
                     /gene="rpmI"
                     /locus_tag="ACHA02_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8671068.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3508..3867
                     /gene="rplT"
                     /locus_tag="ACHA02_002801"
     CDS             3508..3867
                     /gene="rplT"
                     /locus_tag="ACHA02_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8671069.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
BASE COUNT         1338 a          700 c          827 g         1115 t
ORIGIN      
        1 tttaaaaagg ttggagttaa acacaatatt tagtgatgaa gtgagctgga ggtaaatgag
       61 cgtgatcgtt ttagcaggta caattggtgc cggaaaatca agtttaacag aaatgatggc
      121 tgaacacttt gatagtcagg cattttatga atcgattgat gataatgaag tcttgccctt
      181 attttatgca aaccctgaac agtatgcttt tttgctgcaa atttattttt tgaataaacg
      241 ttttgcaagc atcaaacaag caatgaaaga tgacaataat gtattggacc gttcgattta
      301 tgaagattca ctgcttttcc atctaaatgc agatctagga agagcaacag aaacagaagt
      361 tcgtgtgtat gatgaactgc tggaaaacat gatggaagaa cttccctatg ctgcccataa
      421 aaaacatccg gatttattag ttcatattcg tgtatctttt gatacgatgt tggagcgtat
      481 cgaaaaacgt ggtcgttcgt atgaacaatt gtcctttgat ccaagtctat acgattatta
      541 caaagaattg aacagacgtt acgatcaatg gtatgaagaa tacaaagaaa gtccaaaaat
      601 ccaaatcgat ggagaccgct ataattttgt ggaagatcct caagcaaaag aagaagtctt
      661 gaaaatgatc gaagaaaaat tagcagagat tcgccaaacc aaagtagcca tttgatttac
      721 agttgctcaa ataaagggta aacctttatt tgagcgactt ttttctttat actggaaaca
      781 aatgtcagga ggatagagta tgcgggaaaa acactatttt aatcaagacc gtaatttttg
      841 gatcaatgta cagcaaaacg atgtgctagc tgttaaaaac ttaaaagaag aatatggcat
      901 tagtgacgag atgctgactt attctctaga taaaaatgaa cgggcgcgcg tagagtacga
      961 ttcttttgat gaagcgttac tgcttgtatc aaatgttccg caccagcaaa aagtcgataa
     1021 tcattatgaa actagtccta tcgcttttat tttaaaagaa gacggtttgt ttacatttac
     1081 cacgccgaat accgagtacg tgattcgact aattcgatcg cttttggaac gaatgccaga
     1141 catgagtgta tacagtttat tgtttcgtac attgtttttg atttctgatt ccttttttcc
     1201 tttaatcgag gaagtgaata gtgaacgtca gcggcttaat ctgaaattaa gagaaaaaac
     1261 aacgaacaag aatctgctgc aactttcaga tttagagatc ggtttggttt acttagttac
     1321 aggaacaaaa caaaatgtag tacttttaga acaaatcaag gcattggcta tttatcgcaa
     1381 gttgtcagaa aaggaaaaag aagagttgga cgatgcgctg atcgaagcac gacaagcagt
     1441 tgaaatgacg aacttagctt cgcagatttt agaccagctc tctggcactt ataataattt
     1501 gctgaataac aacttgaatg acacgatgaa atttttgacg gtctggtcgc tgatcttgac
     1561 cgttccaact attgtgacag gattcttcgg aatgaatctc cagttaccct ttacccattc
     1621 cgttttcgga tggggaatcg ccttgatcat cagtttggtc ttatctattt ggatgcttat
     1681 tgcactgtgg cggcggattc gttgatctag tctttgatct gcaaaccaag ggcttgggcg
     1741 atcacaagtg cttgctcttg gttgacgatt agtcccgcca aagaatcttg agataaagcg
     1801 attgctgtga agaaattagt cgtaaagtct aatcccttca gagaagttcg aaaccagttc
     1861 gaaccggtca actgattgtc agacaaaaaa aggtttttcc aagtgacctc ataaaattcg
     1921 ctgtcttcta attgacaatg atcgaaggag acagctttca agtttgtact actaaaggaa
     1981 gccatcgaag caatacaatc agtgaaagta cagtctcgaa gataactttc ggcaaaattc
     2041 gtacctgtta atttacattg atgaaacacg acttggtgga aactgccacc aatccactcc
     2101 agattggaaa aatcacattt ttcaaaaatg acattactgc attcaaaatg acgtaaaact
     2161 gttttttgca tcgtaagttt ttggatatga ctctctctta ataccatatt tttggcatcg
     2221 agatagcttt ggtcaccccc gttgatcaga agaccagaga agatctcctc atcttctaaa
     2281 taaaactctc cctttgtaag aagaggaaga gaaggtggaa taggttcgat ttttttcatg
     2341 tgggttcacc tcgtttttct ttagtatagc aaagaagcgg gaatcactgc tagaaagtct
     2401 gctaatctaa tcattttcag aaaaggaaga caaactgtat gaaaaacatt gacaaaaaga
     2461 ttagtttttg ctatactatg atggttgaga aataaaagca gaagcacccg cttctcgcct
     2521 tagttgataa tgtcactggg ctagatagtt ttcactggtt ctattaggtg aagtacgcgc
     2581 gggttctgta agagaactcg tttttttatt gccgtttatt cggtaagctt ttctttctct
     2641 cataatattt ggaggtgaat gaccatagca aaggatatga tggttaacga cggcattcgt
     2701 gcacgagagt tacgattgat cggttcagat ggtgagcaat taggagttaa gacaaaagca
     2761 gaagcattga atattgcaga acaagcaaac ttggatcttg tgttagttgc ccctggtgcg
     2821 aaaccaccag ttgcgcgaat catggactac ggtaaatacc gtttcgaaca acaaaagaaa
     2881 gaccgcgaag cgcgtaaaaa acaaaaagtg atcaatgtaa aagaagttcg cttaagtcct
     2941 acgatcgact tgaatgactt caatacaaaa cttcgtaatg cacgtaagtt cttggaaaag
     3001 ggcgataaag tgaaagcttc tatccgtttc aaaggccgtg ccattaccca taaagaaatt
     3061 ggtcaaaaag tcttgaatcg cttagctgaa gaaactgcgg atattgcaac agtggaacaa
     3121 aaagcgaaaa tggacggacg cagcatgttc ttgacgctgg caccgaaaaa cgacagtaag
     3181 taatttgaat gactaactgg ctgttggaca gccgatagtc ggataacgaa agattttgag
     3241 gaggaaatat agtcatgcca aaacaaaaaa cacaccgcgg atcagcaaaa cgtttcaaac
     3301 gtactggtaa aggcggattg aaacgcttcc gcgcgtttac aagtcaccgt ttccacggca
     3361 aaacaaaaaa acaacgccgt caattgcgta aagcaagcat ggtttctagt ggcgacttca
     3421 aacgtattcg ccaacaacta gcaaaaatga aataagaagt acaaataaca gaatctgaag
     3481 atttattttt attaggagga attatacatg gcacgtgtta aaggtggaac agtaactcgt
     3541 aaacgtcgta aaagagtgct aaaattagca aaaggttact acggttcaaa acacacttta
     3601 tttaaaacag caaaagaaca agtaatgaat tcatacaact acgcatatcg cgatcgtcgt
     3661 caaaagaaac gcgacttccg taaattgtgg attgctcgta tcaacgcagc agctcgtatg
     3721 aatggcttga gctactcaaa attaatgcac ggtttgaaag ttgctgaaat cgacatcaac
     3781 cgtaaaatgt tagctgactt ggctgttaac gatgcagcag catttactgc attggctgac
     3841 caagcaaaag aagctttggc taaataagct aaacaattat aaagaggaaa gaagataacg
     3901 aaaagttatg ttctttcctc tttttttatt tacaatcctc catcaaatga aacaggacaa
     3961 taacgtataa gtctaaaata
//
LOCUS       DAYYDS010000155         3945 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000155 DAYYDS010000000
VERSION     DAYYDS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.161"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(595..1152)
                     /locus_tag="ACHA02_002802"
     CDS             complement(595..1152)
                     /locus_tag="ACHA02_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671070.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1331..1942)
                     /locus_tag="ACHA02_002803"
     CDS             complement(1331..1942)
                     /locus_tag="ACHA02_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671071.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2055..2312)
                     /locus_tag="ACHA02_002804"
     CDS             complement(2055..2312)
                     /locus_tag="ACHA02_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671072.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2909..3187)
                     /locus_tag="ACHA02_002805"
     CDS             complement(2909..3187)
                     /locus_tag="ACHA02_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671073.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3221..3472)
                     /locus_tag="ACHA02_002806"
     CDS             complement(3221..3472)
                     /locus_tag="ACHA02_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671074.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1198 a          809 c          523 g         1415 t
ORIGIN      
        1 aaacgcttct gtcccagcct ccttattccg aataaatggt gtccaaaagc ttgcatctgc
       61 cagattatct catttcttat gacaagctaa gcaaattatc tccaccaaat aatttattca
      121 tatttttgac ttgcgcatac taactaattc ttaaattttc atacaaaatt tctcaatttc
      181 ccttaacgag taatatttat gtacggtgat tctcactagt atcccaatca ctttttaagt
      241 tatcccgatt tcaattctac ttcaaaaata gttgctttta tgtatgctgt tagttgatta
      301 gatggtcatt tgttgagcta ggtcgtttat tttaaacaag ttgtttcttt agcttatacc
      361 tatctcgtct accaaacgca agatggcaaa cacaaatgca tacgcatctg tatttgccac
      421 tttgattcgt ataaagctaa caactattaa ctcctctcag ctaatagttg ctctcttttt
      481 tattcagatt catttttgct gatcacagcc tagtatttca atggttttga acactaaacc
      541 aaattgttcc attacaacat tcactggctg atgaaatgga ttagtagatt tcgattattc
      601 aaagcgattt tctattggtg gatcgtgttc ccaactaata agacggattc caaacctatc
      661 gtacagatct tctaaaccac ttataaattt tgcacctatt tcaggtgttt tattgcgtgg
      721 atattctatt gttacgccat taaatggata ccaattagga ttaggctctc tttttttctg
      781 tacatctccc atagaatagt tagcactttc tgggacataa tatattcttc gaccatcctc
      841 taattcatct aataagatat ttcgtgatcg tctatcactt gctaaatgta gacgccacgt
      901 gcgataccat gccatgctac ctgcatctgc agaaaatcca tcaaataatg caacaattgt
      961 atcttctatc acaatccttt ccccatctac ttcatattct aatctaaaat caaactcccc
     1021 tcgggtaatt tctggtcttg gtggcttagg acccaatatt attctactta aaaccgcatt
     1081 cgttcccaga tgaaacacag ctataacaac aataatgatt aatattttgt ttctatttga
     1141 taaatttctc attattcgca catcacctct acattctcac atattagttc gtaatactcc
     1201 tttaatatca aaatatctac tggtcgtaat aatattcgct atgaattcct actactcgta
     1261 taagaaaggc caaatcatca gcttttactt gctgatgatt tggcttgttt ctatttatcg
     1321 taattgtttt ctaatcacca aaattgttaa caatcggttc agatatttcc cagttgatta
     1381 aggtaattcc gtattgtgtc aaaacttctt gcgcttctcc gaaattaagt acagttctta
     1441 tgtatccact ttctcctaat ataaagtcta cctctctttt aaaaccttcc tcgagatttc
     1501 taagggctat atgtggtttt aatgacattg attgtacatt ttcccccata taatattgcg
     1561 cacttcctcg actaaaatag attcctagat cgtccccaaa aggtagatct tctctaccac
     1621 tagctaatct acttctccat cttcgttctc ttttcataga acctgcgctg aactcacacc
     1681 cgttaaactc agctatgaca gtatcttcaa ttatgttaat ctgtccatct agttcgtatt
     1741 caatcctaaa tggaaattct ccataggtga tttccggttc gggtatttta ggtgagatta
     1801 atgcaaagaa aggaaatatt aagcttggca acatcaaaaa aactcctcct atgatcacaa
     1861 ttcccgatgt aatccaaaat ataatttgta caattctcat tgatccattg tattccattt
     1921 tattccccct attactattc atatccgcct atctatatcg atcttccaat gcagagataa
     1981 ttgcttccat accttgattt tttactgagt tacctatgcg agatatcatt ctccactaag
     2041 gtgcatcata ttgtctatct aacatgatta attctccaat aggtgtatac tcctccaaaa
     2101 acaacattta atgcatctcc ctcgacaaca taagctacca tgtttgctgt atatgtgttt
     2161 gagtctaatt cataagcaga taaatttact gtcgctggat taaatataat cgcattcgta
     2221 tttatagcta ctgaatttgc tgcttcagca ccaccttttg aatgtccgat cattgtgatt
     2281 tcaatagtag gatttacgtg gacagccctc accgtttacc ctgaccatta tagaaatcgc
     2341 ttactttttt gaactacgca acaaaggata caatgctcct aacttcactg tctccatttt
     2401 tgtccactaa ggaatataaa aaataggcat taattcattt aattcggtca caacacgtta
     2461 ccttttaaat cattatactg tatctcatac attattatca ataaactatt ataaaaatgc
     2521 aaaaaatgct ttatcaatct attttgtaat tggtcataat atcattgaaa tcataccctg
     2581 tatcgatttt tcctatgaat ctcgtctatt tgtgattgac gaaaaagagg caagacagac
     2641 actggaatgg taattttacc cactattttg ctccaactat ttgtaatcgt acccactttt
     2701 gaaataccga gatgatcatt ttaattgata tgctgattcc caagcaaaac cccaagaaat
     2761 taaatgctct atcgcatcta gcttcttggg ttttgcttat ataaattcca acatttaaat
     2821 tttttatgct gttcggtctc agaaatgccg tttttgctta tatcacaata tcaagatgtc
     2881 aaattattcg tgttgccgat gactttgatt aatttttttc aaattgttta ttaagactaa
     2941 tgctgtcttt ttgcagatac caatgttcga actttttaac attatcatat gatcctgcaa
     3001 gttttaactt cttaccatta gatgttatca aagttataat taaccttgcc tttttcccat
     3061 taggatcttt tattttatga attttctgca ggtaatctct cctaatggca gctattttta
     3121 cctcatcagt gctgataaac cattgatcat ccacatacat actgcctgtt atgttgtgtt
     3181 tttccatttc ttccgagaac gaaaagttta aggctcgttc ttacttccaa agttgaatga
     3241 gtccatggat tggacttagt gtgagaattg agaacaacaa taagaatcca tatcctcccg
     3301 caaacatccc taaggctata ctgtctctcc atccataagt aggattagaa gagaaaagag
     3361 tcaccgtttg taataatatg aaaaggaata atccagcaca aacaccaaag ataacaactc
     3421 caaacattga atttttatac aatgaccatt tatttatagg cttttctttc attttacttc
     3481 cccctactgt tctgttcaca cggaatgaat tcaaagttca taagccctcc tctttgaaca
     3541 tatgaatacg acttgctaac tatactttaa aaaagcttgt tttaagcggt tataggcgag
     3601 ttgtaatctc tattcatctc attagcaaat aacttgccga aaagtctact gaaattaaag
     3661 cagatagtgg aagaattatt tagccaggat agacactatt tgtgaaacac atgtctttac
     3721 ttttgaaatt ctatgatgaa aagccttgga atacagtagc tcttaattgg taatactttt
     3781 tccattataa ataacctctt ttcattttat atttatcccc tctaaaaata atatctaatc
     3841 taatatttta aacttatatt tccctcgctg tgctaataga gcattactaa ataaatagaa
     3901 actagaaaaa agcattacgc ctgccagaat aaaccaatta tttgt
//
LOCUS       DAYYDS010000156         3905 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000156 DAYYDS010000000
VERSION     DAYYDS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.162"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1430
                     /locus_tag="ACHA02_002807"
     CDS             <1..1430
                     /locus_tag="ACHA02_002807"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8671075.1"
                     /translation="YDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNV
                     LADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVI
                     KKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKE
                     RRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEE
                     MNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKA
                     DFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFF
                     GTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEK
                     YAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEA
                     ISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1420..2637
                     /locus_tag="ACHA02_002808"
     CDS             1420..2637
                     /locus_tag="ACHA02_002808"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8671076.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2650..>3905
                     /locus_tag="ACHA02_002809"
     CDS             2650..>3905
                     /locus_tag="ACHA02_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8671077.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1353 a          669 c          812 g         1071 t
ORIGIN      
        1 aatatgacac agtaagcaaa caaacaatgt tgtatcgtga attactttca gatgaagaat
       61 caaaagaaga tctgattgcc acaatcgttg atattttggg atatgcaatt gcaccagagt
      121 atcttttcaa cgtcttagcg gatcaagcaa aacaagcaac gttccaattg aacgatctaa
      181 acaaagcctt tgtgcaattg gcatctacct acaatcaatt taatgggtta tttgatgatg
      241 tggatctcca atcaaaaaaa ctaggaacag atgagcaaca acgaaatgtg acgattaccg
      301 aagtcatcaa gaaattgaat gatgtagatg ttttagaaca cgatggggac gtgatcgggg
      361 atgcgtatga gtttctgatc agtcaatttg cttcagaagc agggaagaaa gccggagaat
      421 tttacacacc tcatatggtt tctgatatga tggcgcaaat cgtaacgctc gatcaaaagg
      481 aacgacgatt ctttagtgtt tttgatccaa cgatgggttc aggttcattg atgttaaacg
      541 tacgtaacta cttaacacac ccagataacg ttaaatatca tgggcaagaa ctaaatacaa
      601 cgacatacaa tctagcaaaa atgaacttga ttttacatgg tgtcgacgcc gaagaaatga
      661 atctccgcaa tggggatacg ttgaataagg actggccaac agatgaacct tacacatttg
      721 atgcagtagt aatgaaccca ccttattcgg ctaattggtc agcagatacg actttcttag
      781 atgattctcg gtttaatcgt tatgggaaat tagcgccaaa atccaaagca gattttgcgt
      841 ttcttcttca tgggttctat catttgaagg aaacaggtac catggccatc gtcttaccac
      901 acggagtttt attccgtggc gctgcagaag gggtcatccg tcaaaaatta ctagaagatg
      961 gcagtattta tgctgtgatc ggtatgcctg cgaacttgtt ctttgggaca tcgattccaa
     1021 cgacggtgat tgtgttgaag aagaatcgtc aaactcgaga tgtattattc attgatgcta
     1081 gtcgtgaatt tgttaaaggg aaaaatcaaa ataaactatc ggaagaaaac attcaaaaaa
     1141 tcctcgaaac ctacgctgaa agaaaagatg tcgagaaata cgctcatttg gcaacctttg
     1201 atgaaatcaa agaaaatgac tataacttaa atatcccacg ctatgtagat actttcgaag
     1261 aagaagaacc aattgatatg gttcatgtcg gaaatgacat caagaagata cgacaagaac
     1321 agcaagtgct agaaaaaaaa cttttagaag ctatctcttc cctgcaaaca acacctgaaa
     1381 atgaagcgtg gttacaaggt gcgctggagg tgttcaaaca tgagcaatga tacacaacca
     1441 gaaatccgtt tcccaggatt tacagatgat tgggaacaga ggaagttggg ggacttcgtt
     1501 gtagattatg ttgaaaagac aagtgtacaa aatcaatttc ctatgctaac ttcatctcaa
     1561 caaaaaggga tagttttgca agaagactat tttgcaaatc ggcaagtaac tacagaaaat
     1621 aatattggtt actttgtact acctagagga tatttcacgt tccgtagtcg aagtgataat
     1681 gatgtgtttg tgtttaaccg taatgacatt attgatagag ggattataag ctatttttat
     1741 cctgtattta ctttaaaatc tgctgattcc gacttcttct taagacgtat taacaatgga
     1801 atccaacgac aattatctat tcaagcagag ggaacaggtc aacatgttct gtctctaaaa
     1861 aagtttaaaa atatagtagc aatgtttcct agtgaggaag aacaacaaaa aatcggcacc
     1921 ttcttcaaac aacttgacga cacgatcgct cttcatcagc gtaagttaga tttgctgaag
     1981 gaaacgaaaa aaggattctt acaaaaaatg ttccctgaaa acggagcaaa agtgccagaa
     2041 atccggtttc ctggttttac ggaagattgg gaagagcgta agttgggaga attaggtaaa
     2101 actcaatcgg gaattggatt tcctgatgcc gagcaaggtg gttctgaagg tactccattt
     2161 tttaaagttt ccgacatgaa taacattggt aatgagtatg aaatgagaaa tgcaaatcat
     2221 tatgtatcta atgagcaaat tgagcgtaaa aaatggaagc caataaaaga tgtgccagcg
     2281 attatatttg ctaaagttgg cgcagcaata atgcttaata gaaaacgttt ggtcacaagc
     2341 cctttcttaa tagataacaa taccatggct tatttattta ataatacttg ggatatctat
     2401 tttggtaaaa tattgtttga aactataaat ttgccaagat actcccaagt aggggcattg
     2461 ccttcttata acagttctga tattgagaat atcagtgtta aggttcctgt aaaggatgaa
     2521 caacaaaaaa tcggtacttt cttcaaacag ctagatgata cgatcgctct tcatcaacgt
     2581 aagttagatt tactaaaaga aacgaaaaaa agcttcttac aaaagatgtt tgtctaggag
     2641 ggaagaagaa tgaaaattag tcaccgtgag gaagcagaag tcgaagaaca gttgattcgt
     2701 gtacttggag aagggcataa tcagtggacg tatcgtccgg atctaaaatc agaagaagac
     2761 ctttgggtca atttacgcca aaaaattatc tcaaataatc aagcagaact gaatgatttt
     2821 cctttgaccg ataaagaatt tgaaacgatc aaaacggaat tactgctgcg aacgaaaacc
     2881 ccatttgatg ctgcgaaatg gttaaaaggt gaaaatggga tggctcggat cacgattgaa
     2941 cgggaagatt cgcaattggg ttctgtctca cttatccttt attccaatca agacattggt
     3001 ggaggaatct ctacttatga agtggtccat caaattgcaa aacgtggaag taacatagaa
     3061 gcacgggatc gacgttttga cgtcacctta ttaatcaatg gattaccgat tgtccaaatc
     3121 gagctgaaac aagtaactgc aaaagatggt gtttatcagg catttaacca aatcaaaaaa
     3181 tatgcagaag aaggtatgtt tcggaacaat atcttttcga ccttacagtt gtttgtggtt
     3241 tccaatgaac aaaccactcg gtattttgca aatgccttac cgaaagactt gcaccctaag
     3301 tttttattta gttggcgaac gaaagacaat gaaaaagtag aaaacttata tgagttttgt
     3361 aaacaagtgt taaatatccc ggatgctcat cgtttgattg cggattatac aatcgtcagt
     3421 gaagatcaag acaataaaac cctcatggtg ttacatcctt atcaagtaca tgcgattcaa
     3481 gccttgttta ttgccgcaaa taaacatcaa tcagggtatg tgtggcatgc gactggttca
     3541 ggaaagacgt tgactagttt tgtatctact aagcttttag cacgaaaatc ggggatcgat
     3601 cgaacgatca tgttagtgga ccgcaaagat ttagataatc aaaccaccac agagtttaca
     3661 aaatttgctt cggaatttaa tactgggatt tcttccggga acgccaaagc gaatagcttg
     3721 atcgttggaa cgggaagtgc caaagaatta agtgaaaccc tgttggcaga tgccaatgca
     3781 aatgttgtaa tcattacaac ccgtcaaaaa ctagatgcgg cgttgaaata tgcaaagaaa
     3841 caagaagaga aaaaaggaac caatcgcttc caaaaattaa tgggccaaca tatcgtgttt
     3901 gtagt
//
LOCUS       DAYYDS010000157         3872 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000157 DAYYDS010000000
VERSION     DAYYDS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.163"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2257
                     /locus_tag="ACHA02_002810"
     CDS             158..2257
                     /locus_tag="ACHA02_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8671078.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="ACHA02_002811"
     CDS             2453..3316
                     /locus_tag="ACHA02_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8671079.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3872
                     /locus_tag="ACHA02_002812"
     CDS             3421..>3872
                     /locus_tag="ACHA02_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8671080.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
BASE COUNT         1540 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa ga
//
LOCUS       DAYYDS010000158         3713 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000158 DAYYDS010000000
VERSION     DAYYDS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.164"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHA02_002813"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHA02_002813"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8671081.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACHA02_002814"
     CDS             complement(2261..2512)
                     /locus_tag="ACHA02_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8671082.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACHA02_002815"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACHA02_002815"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8671083.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACHA02_002816"
     CDS             3462..3680
                     /locus_tag="ACHA02_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671084.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DAYYDS010000159         3468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000159 DAYYDS010000000
VERSION     DAYYDS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.165"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHA02_002817"
     CDS             complement(<1..2232)
                     /locus_tag="ACHA02_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8671085.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHA02_002818"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHA02_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          974 a          769 c          548 g         1177 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaatcactac cagtcaac
//
LOCUS       DAYYDS010000160         3388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000160 DAYYDS010000000
VERSION     DAYYDS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.166"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2555)
                     /locus_tag="ACHA02_002819"
     CDS             complement(63..2555)
                     /locus_tag="ACHA02_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8671086.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ACHA02_002820"
     CDS             complement(2562..3029)
                     /locus_tag="ACHA02_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8671087.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3165..3238)
                     /locus_tag="ACHA02_002821"
     tRNA            complement(3165..3238)
                     /locus_tag="ACHA02_002821"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3202..3204),aa:Arg,seq:acg)
     gene            complement(3296..3369)
                     /locus_tag="ACHA02_002822"
     tRNA            complement(3296..3369)
                     /locus_tag="ACHA02_002822"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3333..3335),aa:Pro,seq:tgg)
BASE COUNT          924 a          750 c          557 g         1157 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tcgacgcgcc caagaggagt
     3181 cgaaccccta accttttgat ccgtagtcaa acactctatc cagttgagct atgggcgcat
     3241 attcaattta aaaaaattat aaatcatttg aaaacaaaaa gcaagataaa aaaaatcggg
     3301 aagacaggat tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc
     3361 tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DAYYDS010000161         3317 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000161 DAYYDS010000000
VERSION     DAYYDS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.167"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..1043
                     /locus_tag="ACHA02_002823"
                     /pseudo
     CDS             73..1043
                     /locus_tag="ACHA02_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2556..>3317
                     /locus_tag="ACHA02_002824"
     CDS             2556..>3317
                     /locus_tag="ACHA02_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8671088.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRP"
BASE COUNT         1204 a          497 c          637 g          979 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      721 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      781 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      841 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      901 gtagatgaat tttttattca atctgtcgca tctaaacgaa ataatattcc tagaaaatca
      961 ctggattatc gaacgattat cgaacacctt tggaagtatt tttgagttac gtaagtattg
     1021 atgatctgtc taatttaatt tgacaattaa gaaagtatta acttgttttg tgaaaaagag
     1081 aatttgaaaa acgattggcg aacaaatgct cggtaggtat ggtataaata cagagctaat
     1141 tggtgtgatg aaatcagata ttcgcgtgag attcagtaaa gtaggagttc gaaaacagct
     1201 aggtgacaaa cagtattgct ttggtacttc ggaatggttt atcaaattct tatcaacagt
     1261 tcacttctct ttatgtttga gtgatgattc gttaggttat tgcttggaaa gaccggatga
     1321 gagtagaaga gtttaatttt ataaagtgaa aagaggtaat gtacgatata cgtagggctg
     1381 aaccaataga ggataagcaa acgatgttac aggcgcaaaa ttcatagctt actgttttgg
     1441 agataacaga agaggaaata atgcagaagt aaggcgaaaa aaggacggaa gcctaacagc
     1501 atatgaaata aagaaaaata ttataactac tgataaatcg gtattgagca aagtactaat
     1561 aggagtcaaa aacattcata agctatgagt gtttcttggc tactttttta tttttatgta
     1621 tctacaaaag tttccagggg cagaacctgt gattattaat ggaagaacct caagtattgt
     1681 cttaaatcat tgttatgaaa atggattaaa agagttcatc aaggtgtgat taacaagtta
     1741 cttttcatta catcgaattt ttcagaagtt gcttatattg gtgatgatat taatgattta
     1801 tcatgtatga catctgtgaa gaagcgggag gaatagtaga atgtccaaaa aatgccacaa
     1861 gaaatgagat tgaattatct gattttattg taactcacag tggtggtgac gaatctgtga
     1921 tagagtttat tgagtggata ttggaatcga tatagtgtag tagtacagaa aaattatcat
     1981 acaattaata ctaggcataa gaatattggc atattctaga gttaaaagta actattcgaa
     2041 aaggataagg ttagaatttt ttgttttgcc agtggaagaa tataaaaagg gcattggaaa
     2101 ttaatatatt agaatgctaa gcggagaaaa aaatatatga aaagtacgtc cttgtgatgg
     2161 gatggaaaaa tacacttatt tcaaacaacg ataatataat tatgtgaacc gaagtatact
     2221 cgcttctaat tcaatcctca aatcccgaca gtcgcgactt caaaaaagcg tatagtttta
     2281 atgtatcccc atatgatccg aaaaatggta atttacaatt aacagtttga tgatgttgaa
     2341 gatgtggaaa tgctgaaatt accatgatga gaaacacatt aattaaaggg aattaattaa
     2401 ctagaatata atctgattca atttgaatta agcaaaaaaa ggctgagttt taaataatcg
     2461 cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc aaattgtatt
     2521 tccgacgaaa gaaaacaaag gagtgtaacc cataaatgac ctacaaacat cttaccatag
     2581 acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt gaaatcgcta
     2641 accgaatggg acgtgctata caaactattt ataatgtagt caataagttc aagcaaggca
     2701 agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt ggtagaaaag
     2761 tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact cttggttgga
     2821 cgcctgacgt cattatcggg cgaaaagaaa ggcctgtttc atgcggtatg agaacacttt
     2881 atcgtttatt ttctaaagga atatttgata ttgacacact accgatgaaa ggtaaaagaa
     2941 aacccaatgg ccatcaggaa aaacggggaa aacaacagta tcagcgctca atccatgatc
     3001 gacctgataa ttatcctgat ttcaattctg agtttggtca ccttgaaggt gatacgatcg
     3061 ttggcattca tcataaaagt gccgtcatta ctttagttga aagattatct aaagtcatta
     3121 tcacgattaa acccaacggc cgtaaggcat tagatattga aactgccctt aatcaatggt
     3181 tttctcgctt ccctaaaaac ttctttaaat ctattacgtt tgactgtgga aaagaatttt
     3241 ctaactggaa agccattagt aaccaacatg atattgatat atattttgcg gaccctggaa
     3301 caccttctca acgccca
//
LOCUS       DAYYDS010000162         3215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000162 DAYYDS010000000
VERSION     DAYYDS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.168"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(435..1049)
                     /locus_tag="ACHA02_002825"
     CDS             complement(435..1049)
                     /locus_tag="ACHA02_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8671089.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1655..2032)
                     /locus_tag="ACHA02_002826"
     CDS             complement(1655..2032)
                     /locus_tag="ACHA02_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671090.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2332..3213)
                     /gene="galU"
                     /locus_tag="ACHA02_002827"
     CDS             complement(2332..3213)
                     /gene="galU"
                     /locus_tag="ACHA02_002827"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8671091.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          978 a          659 c          497 g         1081 t
ORIGIN      
        1 tgtattttat ttctattaga aaaaggtatc ctgttggaca attttgttat atcaggtaaa
       61 cttcattgca ataatgataa caaatgtact ataattactt tgtaaagagt tttggggaag
      121 actctttcat caacgtgtat taagagacaa gaaagaatcc atatggattc tttcttgtct
      181 ctttttccac tactttttat ttttctgaca ctacatattc ttataactca tttctcataa
      241 ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa atcaaagatg
      301 atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca ttaataatcg
      361 ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa ggaactttgt
      421 caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc tgtaaaatac
      481 aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat cgctggatct
      541 agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa aaccaaatca
      601 gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg tgacaaatca
      661 aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc tagttcgatg
      721 attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga tttgacagaa
      781 agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc tccggctgga
      841 ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc aaatgctaca
      901 ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat ttgtttcgcg
      961 atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc ttccaattgg
     1021 tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat acttgacatg
     1081 aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta gacagtaaca
     1141 catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc ccaatacctt
     1201 gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg gtagaatttt
     1261 caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg gcaaaatgat
     1321 cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa ctaagggtca
     1381 gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat ctcttatcca
     1441 agtgcaagca agaaaataca aaaaaacatt ggtatgattt atatatatat aaataacata
     1501 aaaaaaggaa tggcttatcc cgatacaaca agcattcctt taaaaaagat tcttttatat
     1561 aagtgaaaaa agcttttttc caccaattaa ataaatctct catctcaata gtttatccat
     1621 tattttatct ttttttgcgt atacctacta aatattaccg tcttcttctc gttcttctac
     1681 tttgttcgag agaatctgta ctagaaaaag tagttgtatg tctacctttt ctgcctatcc
     1741 gagactttct tttcatatat gggatataaa tgattagata cagtacagca aaaaataaag
     1801 caaatacgca agcttcccaa ctacctaacg ccaattcaac catcaaggta gcaaccccaa
     1861 cggatgctgc ataccagata aaaatcagaa acgtttcttt ttttctgacc atgcgaaaca
     1921 tgataaatag actaataagc aatagtggca acacaaatat ccaattgctt ccttccatca
     1981 gccacttgag tatatcaagc agcgactgtg caacagcgac taattttccc atagtgaaca
     2041 cacacctttt tttataatta aaaaattcgc ctttttattc tactatgaat cttctggctt
     2101 ggctttcatt tctcttatgt aatcattttc ttttatacaa cttaaaaaaa aattaaacca
     2161 ttagcataat attttctgaa tctattcacg aaagtctttt tctatctttc gttttccacg
     2221 actcaactga tctagagtca aaaaactaaa acgacagttg cgcaaatgac gcacaagctg
     2281 ccgctttaat aacaaatagc ctttcattct cagcaggcca cttatttcca gttaactttc
     2341 tgttcttagc tttttactca actggataat atatgcactt aatgaagtcc caatttccga
     2401 atgttgaagt ccatattcaa tatttgtctt catatatcct aacttatcgc ctacgtcgta
     2461 tctttttcct ttaaactctc tcgcatagac cgcctctgtc tggttaagag tctgaagtgc
     2521 atccgttagc tgtatctcgt ttcctgctcc tggttcttga gtttctaata tctcaaatat
     2581 tttaggagtc agtagataac gtccgataat ggctaaatta cttggtgctt ttcccatttc
     2641 aggtttttct acaaaccctt ttactctgca acgacccttt tcgctatctg ttttaggatc
     2701 aataattccg tatttagatg tttcattttc tggtacacgc atgacggcaa cagcggaagc
     2761 ttgtgtttct tcataatcca tcatcaattg ttgagtcaat gggatatcat ctttcatcaa
     2821 atcatccccc agcatgacga caaacggttc atttcctata aatgcttttg cttgaagcac
     2881 ggcatccccc agtcctcttg gatgagattg acggatgaag tgaagattga cttgagtcgt
     2941 ttcttcaact aaagcgagta attctgtttt ttccttttcc cgcaaattgt tttccaattc
     3001 gatatttgca tcaaagtgat cttcaatggg tcttttttct tttcccgtta cgatcaagat
     3061 gtcttcgatt cccgaatcca atgcttcttc cacaataaac tggatcgttg gtttatccac
     3121 gattggaagc atttcttttg ccatggcttt tgttgccggc aaaaaacgtg ttcctagccc
     3181 agcggcagga atgactgctt ttttgacctt cactt
//
LOCUS       DAYYDS010000163         3213 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000163 DAYYDS010000000
VERSION     DAYYDS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.169"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..443)
                     /locus_tag="ACHA02_002828"
     CDS             complement(<1..443)
                     /locus_tag="ACHA02_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8671092.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNIS"
     gene            513..863
                     /locus_tag="ACHA02_002829"
     CDS             513..863
                     /locus_tag="ACHA02_002829"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8671093.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            1277..2095
                     /locus_tag="ACHA02_002830"
     CDS             1277..2095
                     /locus_tag="ACHA02_002830"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8671094.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(2168..2527)
                     /locus_tag="ACHA02_002831"
     CDS             complement(2168..2527)
                     /locus_tag="ACHA02_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8671095.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT         1059 a          475 c          574 g         1105 t
ORIGIN      
        1 tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt
       61 cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc ccgttttaaa ccggcgatag
      121 agggtactta ctgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc
      181 caaccttcag ttgatctttt tgcaatgtat tctttttcct cagcaggaaa aatgatttcg
      241 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
      301 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      361 gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      421 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      481 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      541 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
      601 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      661 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      721 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      781 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      841 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      901 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      961 tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca
     1021 aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca
     1081 ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa
     1141 atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag
     1201 taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg
     1261 cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat
     1321 ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt
     1381 atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca
     1441 attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc
     1501 ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga
     1561 ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa
     1621 tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta
     1681 ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt
     1741 tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt
     1801 agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga
     1861 gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca
     1921 gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag
     1981 ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc
     2041 aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact
     2101 aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag
     2161 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
     2221 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
     2281 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
     2341 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
     2401 tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
     2461 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
     2521 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
     2581 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
     2641 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
     2701 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctattttc tacaatattt
     2761 aaaacgcttc cttagtctat tatatatcta tttctcttag gagtatgttt agatgtttga
     2821 ataatagttg cccaacttta aaggttattc ttataatatt ttacttcatt ttccaataga
     2881 aaattcaaag gattactggt ttttctaaaa tagtttagtt taaatacata agtatcctcc
     2941 gacactgtat tagttcataa tcatctaaat acaaaatttt caaataaaaa tatttcaagc
     3001 tctatcgttg acgaatgtaa acgatactta taaagtaata gaaattgatt acatttgtaa
     3061 acttaaaatg gttaatttat ctcacataaa actaagaata aaataatgat taaaagtggt
     3121 tagtttttat acgatttagc ctatcggtaa aaatgaatta gagagtgtaa aatattttgt
     3181 gtaaatgaaa aaatccatac aaaaaaggaa gtc
//
LOCUS       DAYYDS010000164         3131 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000164 DAYYDS010000000
VERSION     DAYYDS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.170"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(274..498)
                     /locus_tag="ACHA02_002832"
     CDS             complement(274..498)
                     /locus_tag="ACHA02_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671096.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            864..1148
                     /locus_tag="ACHA02_002833"
     CDS             864..1148
                     /locus_tag="ACHA02_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671097.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1151..1567
                     /locus_tag="ACHA02_002834"
     CDS             1151..1567
                     /locus_tag="ACHA02_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671098.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1704..2423)
                     /locus_tag="ACHA02_002835"
     CDS             complement(1704..2423)
                     /locus_tag="ACHA02_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8671099.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
BASE COUNT         1046 a          582 c          485 g         1018 t
ORIGIN      
        1 aaaaagcccc agagaatccg acctctctgg ggctttcgtg tgtttcgtgt tcacttattt
       61 gcaatcaaat tataacagaa ataaatattt atttcaaaaa tatcctacaa aagtctaaaa
      121 tccgtaatta tttcttatat gcaacattta caaataaagt cgctctgaga gctcgtattt
      181 ttcgatttaa gacgttttat ttagtttttg gtgtatttca ttggtccaag ctaaaaaact
      241 cttagaagcg attttagagc catttaaacc cacttaatta tattctccat gaaattcgct
      301 ccatgcgtca aaagctgcgt ctgaaattcc gccgtcttcg ttcactagtt gcattaattt
      361 ttccaacgat cgttttcttt cgggtgtcat atttttatat tccaacaatt tttcgtctgt
      421 taaatcgaac ttgattgctt gaattgcttg ctctaatttt tctaagtttt gaaaatattc
      481 gtaaggaaat ttttccattt tttctttctc cagtctcaca atttttctag aaataaaatc
      541 acttctttgc cgtctatact ctcaaaaaaa atgtcgtttg aaaaactaaa taaatttttg
      601 atcgtgcatt tttaaatttg gttatcgatt acacgtgcag cactcccccc cgaattttaa
      661 agagcataag gaaacgtgta cccactttct aaaaaaagtg ggtagatagg ctgtgcctat
      721 cacgtttctc agaaaaaaat ttgatatcac tttttcgcca tcgtgggtag cttatgtata
      781 gcattttggc aaaaaacagc tatacaaata tagtatttgt atggtataca taaattatac
      841 ataattgcaa aaggagattt tttatggaca ttttagttcg gaatattgat ccaaagtttg
      901 ttgcaacaat agacaaacgt tgccaagaac tcacgaataa aacaggagaa aaatggagtc
      961 gaaataaata tctaaaagtt ttaatcgaaa gtgattttga ccgtcaactt acgacttaca
     1021 aaaaagacca attcgattta ttgctcgata aatttattgc tatccaaaca gaaaatactc
     1081 aaatattaag cgagtatgtc tacacaaata atcaaatcat tgaaatgctt atcggaggaa
     1141 tgatataacc atgagaaaaa cttctgtcca aaaacatgtt actttgtacc ctagtcatgt
     1201 tgacgtttta gattccatca tcgaaaaaaa tccagatatc aataactact cggaagccat
     1261 tcgcttttta tgtatgagtt atgaacccga taccaacacg accactacca gcaatcaggc
     1321 aaaactaaat gctatgggaa aagaaatttc catcatgact gaaatcatcg cagaatttgc
     1381 tgatttatat ctaaaagata caggcgttct tattggcaca aattctaaag tatatcaaga
     1441 agtgaaagaa aaaatcgaag ctaaactaaa tccatcaaat cgaaatcgac caaagaatca
     1501 tcaaaaagaa accagtcaaa cagtcacttc tagaaatttc gacccaacaa attttctaaa
     1561 acgataaaaa acagatagga gttattccta tctgtttttt atttagaagc cccatacaat
     1621 atgctagaat gtactagcaa tcttagtcgg ttgtaaggga cttgtctact tgcttaatgt
     1681 aaggggggcg agttcttttt tatttattct tctaaccagt tacgcagaga aatctttggc
     1741 aaatctgacg ttaggcttga aaaagtaaat tctaatttgg cgattttatt gccctttttt
     1801 gcttttattt ttgtgacttt aagatcattg aagtattttt ttagttctcg taatgcagga
     1861 tttaatactt tttgatcaat gttacccatt tgatagtatt ctgggatatc cattaagctc
     1921 ctaaactctt caatacgaac tacataataa ccagtagaac ggtactgcat aagcagtcgg
     1981 aatagagttt ttgcatacgt agaacgcaac gacgtgaaag cagataattc aaatctacta
     2041 aattcagctg ttaaaccatt aataatatat tctaggtccg gattaacact gacttcaaca
     2101 aacttttttg ttttattgat ttcaaaacca gtaaacaaaa caaagtagcg aatagaatcc
     2161 tcatcttcag tacggtaagt aagattaagc atatctttat agagcttttc taaatcaccg
     2221 acaaaggctt ttgtagcagt catcttataa tcacttaact cttttaattc ctcgaaagaa
     2281 aatctaactt ttttcaaacc acgatctttc atacgagcgc ataatgcaaa aaataaatcc
     2341 atctgcttag cattgaaatt tttcagcggt actaaattca attcattttg atatttgact
     2401 gttaattccg ttttttcatc cattcctttc ttcccccctt acaaataaat aataaccttt
     2461 aacaaccaac tcgtaaatag gttggttgtt attatacaaa aggatattag tactcagata
     2521 aaaagttgtt agtactcaga taaaaagttg ttagtactca gataaaaagt tgttagtact
     2581 cagataaaaa gttgttagta ctcagataaa aagttgttag tacccttctg tatccgttgg
     2641 gagagtaagg ctcaactgcc ccctaaaaag ggttctaaaa aatctttaaa aaataaataa
     2701 aataaacgcg cgcgcgcgag caaaatttcc cgccaaaaaa tcacccactt tttaaaataa
     2761 acaggttcgc gcatgatatt aaactgccaa tctagctccc tatggtcgct gtaagaagga
     2821 gtgatcgcta cgctctcaca ttttacttgc agcagtcgcc tttaggctcc ttcgccaaaa
     2881 ataattcatt agggactcct ttgtggcgtc ctgagagctc gtctaagccg ttctaagaca
     2941 agattcgata aaaactatgt ccttcgttct aaagcgcctt acaggcggtc agggcaagcc
     3001 ctaacaaccc caaggcaata aaaaacttag gcttattttt gcttttttat tgtataattg
     3061 attgtcgcct atccgacagt cttgcgactg gaaggaggtg tgtttcgtgt tcactaactt
     3121 tatttgctcc c
//
LOCUS       DAYYDS010000165         3128 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000165 DAYYDS010000000
VERSION     DAYYDS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.171"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1347
                     /locus_tag="ACHA02_002836"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ACHA02_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="ACHA02_002837"
     CDS             1360..1992
                     /locus_tag="ACHA02_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8671100.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2200..2424
                     /locus_tag="ACHA02_002838"
     CDS             2200..2424
                     /locus_tag="ACHA02_002838"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFM8671101.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2526..2759
                     /gene="relB"
                     /locus_tag="ACHA02_002839"
     CDS             2526..2759
                     /gene="relB"
                     /locus_tag="ACHA02_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8671102.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="ACHA02_002840"
     CDS             2749..3018
                     /locus_tag="ACHA02_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8671103.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DAYYDS010000166         3055 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000166 DAYYDS010000000
VERSION     DAYYDS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.172"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..351)
                     /locus_tag="ACHA02_002841"
                     /pseudo
     CDS             complement(1..351)
                     /locus_tag="ACHA02_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(611..1159)
                     /locus_tag="ACHA02_002842"
     CDS             complement(611..1159)
                     /locus_tag="ACHA02_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671104.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1160..2017)
                     /locus_tag="ACHA02_002843"
     CDS             complement(1160..2017)
                     /locus_tag="ACHA02_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8671105.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2303..2589)
                     /locus_tag="ACHA02_002844"
                     /pseudo
     CDS             complement(2303..2589)
                     /locus_tag="ACHA02_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2579..2908)
                     /locus_tag="ACHA02_002845"
     CDS             complement(2579..2908)
                     /locus_tag="ACHA02_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8671106.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2985..>3055)
                     /locus_tag="ACHA02_002846"
     CDS             complement(2985..>3055)
                     /locus_tag="ACHA02_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8671107.1"
                     /translation="YKVDEDEHVVEIYSARTHYESQ"
BASE COUNT          894 a          620 c          384 g         1157 t
ORIGIN      
        1 atgttcttac tgcctcttcc acactaattt cttcgttcaa tttcactaat aatacttgat
       61 ctcgcttatt ataatcgctt tcatttatcc taagagtcaa atctttttcc accataactt
      121 ttacacccaa taaattttgc ggtttatttt tctctgttga aattctttgt gtagccttta
      181 aactgacaat tagctcattg ggtacatact tatttgagag tcatctaatt ggctcataat
      241 cctttctttt tggttagtat ctgcagttac ttgtttagta tgagccatga acccaaacat
      301 actcacccca acaaccaatc ctaaatatat tttcttttta aactttttca tctacatctc
      361 ctcctaatag ttttatttta gagttagctc tattacttat ttactaactc aacaacagat
      421 tatcatacac ttgttaatga aatcacaata tctcattaaa acacacccta aaatcataac
      481 cgttcctatg ctcatttttt ggatttttta atcacgtaac cagaaaaata aatcccctct
      541 atttcacgca aatcaaagaa gaactaagta actaccgttt aaaggtagtt acttagttct
      601 tctttgatta ttatctaact ttattattca ttccccaccc tttgcgcagt gagtcttcgg
      661 tatcttgttt tatctgactg gtagttctta ttatttctcc acgatcatct aattctggaa
      721 caattggata tttttctctg aacaagtcta gttctttcac cacgtcatta aacaaatctt
      781 gcttatcttc ttgcgtaaga ttctgtttaa tatactcatc taaagctttt ctaagcaatt
      841 catctcttgt catattagtc tcatactttt ctactaaaac tctggaagtc gcatttaaca
      901 aagtcgacag ttttaaagga tgcttggtgg aataaacctt atcaaacttt ctgattggtg
      961 cgccaacttt ttttgttctt cctctactct gtctttcaac attggaggaa gtttttattt
     1021 cttgaacttg gtgcacttct aattgcttat tttcagatgg tgtaattgct tgctgctttt
     1081 tctttgcttc tgcttctttt tttattttgg cgagtccttt gttgttccca aaaatatcaa
     1141 gtcctttgat gtcagccaat tactccacct ccgcatacca attctcgtgt tcttccaatt
     1201 ctttcaaaat actgataaat aattcgtggc ttttgcgatc ccattgtttt aagagacctt
     1261 tctttgtctt ctccatcgta atacctgatc cgtcgtatcg ttttaagcgc gcatttttga
     1321 gtacaacatt tttaatcaaa tgttccccat aaatctcttg ggcttgttta tacatttcct
     1381 gatctactgc gtcaccgctt tccaacatga aaggaataac accagcaaca tataaatttg
     1441 tatcaaattc tttcacaaaa aagtccatat actcaagata ttgcatcgca tttctcatcg
     1501 attttacttg ggtctgtaag acgacgattg cccaatccgc aatgtacatc gcggatttac
     1561 tatatacaga aatagaaggt gggacatcta aatagatgac gtcaaactca tcaaataatg
     1621 gttctagctt ttctttcata tatttgaatt gggataatga atcattatat tggtctttga
     1681 tccaatcatc atacatttca aaggacatgt ctgctggaag tatatagagg ttattgatta
     1741 ctttatagac agcctcttta ggtggaagat tgtttatcaa cacgtcagtc acagtatttt
     1801 cgtaaacaat cggttcacct tctccgcctc cttcttcgtt agtgaagttc atatgcgtta
     1861 agttcatcac atcagaagta tttccttgca tatccaagtc aattatcaag gttctttttc
     1921 cttcattaac tgctgattca aaccctaaca tttgcgcgca cgtactttta cctacgccac
     1981 ctttaaaatt accgattaca tatttcttcg ctaccacgtt cctacctctt ttcttttagc
     2041 aatattcatt tgatactatt ataacatatt tatttttagt tactaccaaa aaaaagatat
     2101 atatgtacta ccttataatt aataagtaaa aggtagtaca taaataagga aaagctgaaa
     2161 tatattgatt tatagctttc ctcaaagtaa tgacgttatt taatttatcg agaagcagag
     2221 aatactcgcg actttagtca tgtagacgaa tcagattttc gataaaaaag aagccgtcct
     2281 tcttaagaac gacaacaaat cactagatta attttttcaa ctgttcataa aagttttcat
     2341 gcgttccaca taacaatact ggaataacct tgttttctat aatctcgtaa gctactctat
     2401 atgttgtgcc cgcatatttg aacccactag accaaatacc tgataaatcc ccagtttttt
     2461 gttcaccctt atatggatca atcgcaattt catcataaat taaattcaaa aatttttctt
     2521 tcaatggctt ctcttttagc tttttaaata cttaatgaca tgttttgaag gaacgatttc
     2581 ataagccaat ttctgttgcc aactcctctt tagtcattac tttagaatta gtaagtgtat
     2641 cttcagcaat atctctaaag gaagaatgca ttttgttttt tctattctta aattctacaa
     2701 gaatctcatc tttatcgtag ccttctgcaa ttatatcaga caaaatatct tcgctaaaat
     2761 cataaaattc tttttgtggt tcaacaaatt cataaagaat accattctct accaactttg
     2821 cttcaacttc gactccatta ggaacattaa aatctttagg tactgtcaac atgattgaat
     2881 tgccttgttt tcttgttttt gtaatcattt aaatccctca tttctaacag tatgtaatta
     2941 cttagtaaat acattatacc acgagataat gtatttacaa gtaattactg tgactcgtaa
     3001 tgcgtccttg ctgaataaat ttcaaccacg tgttcatctt catctacctt ataaa
//
LOCUS       DAYYDS010000167         3012 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000167 DAYYDS010000000
VERSION     DAYYDS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.173"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..359
                     /locus_tag="ACHA02_002847"
     CDS             177..359
                     /locus_tag="ACHA02_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8671108.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="ACHA02_002848"
     CDS             462..746
                     /locus_tag="ACHA02_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671109.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="ACHA02_002849"
     CDS             762..998
                     /locus_tag="ACHA02_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671110.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="ACHA02_002850"
     CDS             1020..1274
                     /locus_tag="ACHA02_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671111.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="ACHA02_002851"
     CDS             1356..1541
                     /locus_tag="ACHA02_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFM8671112.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="ACHA02_002852"
     CDS             1608..2003
                     /locus_tag="ACHA02_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFM8671113.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            2020..2370
                     /locus_tag="ACHA02_002853"
     CDS             2020..2370
                     /locus_tag="ACHA02_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671114.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="ACHA02_002854"
     CDS             2392..2766
                     /locus_tag="ACHA02_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671115.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          924 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc tt
//
LOCUS       DAYYDS010000168         2947 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000168 DAYYDS010000000
VERSION     DAYYDS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.174"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1371)
                     /locus_tag="ACHA02_002855"
     CDS             complement(61..1371)
                     /locus_tag="ACHA02_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8671116.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ACHA02_002856"
     CDS             complement(1383..2801)
                     /locus_tag="ACHA02_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8671117.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          931 a          609 c          456 g          951 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagat
//
LOCUS       DAYYDS010000169         2927 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000169 DAYYDS010000000
VERSION     DAYYDS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.175"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1364)
                     /locus_tag="ACHA02_002857"
     CDS             complement(45..1364)
                     /locus_tag="ACHA02_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8671118.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="ACHA02_002858"
     CDS             complement(1361..2014)
                     /locus_tag="ACHA02_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8671119.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..>2927
                     /locus_tag="ACHA02_002859"
     CDS             2302..>2927
                     /locus_tag="ACHA02_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8671120.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          959 a          477 c          323 g         1168 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgaca ccttctgttg
     2101 ccaatacttt ttcttttttc taataaatgc aaacagacac cttgaaatcc tttagaataa
     2161 agggttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tattgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaaactaa tcgctaagaa gtacttcgtc
     2701 tcggatggga cggtgacacg gatcttgcgt gaagcaacga agcattacca gccacgtatg
     2761 aacttccttc cgacggtgtt gtgtatggat gagttcaaat caatgaagtc tgtttctggt
     2821 tccatgagtt ttatttgtgt tgatgggaca acccatcagc tatttacgat ccttgaagat
     2881 cgcagacttt acaaactaac tcaatacttc ctgcgctttc ctagaaa
//
LOCUS       DAYYDS010000170         2884 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000170 DAYYDS010000000
VERSION     DAYYDS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.176"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            558..>2884
                     /gene="esp"
                     /locus_tag="ACHA02_002860"
     CDS             558..>2884
                     /gene="esp"
                     /locus_tag="ACHA02_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8671121.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1044 a          441 c          565 g          834 t
ORIGIN      
        1 atcaataatt taatgaggct gtgacaaatg tcattttttg tttcgctaaa aacgagtgaa
       61 aaaatgttga taactccaat cattcgttta agattctagg aatcattcag aaatgctgtg
      121 aaaccgatac tttataatta taaagaaggt gatttcaaat tccgaagaag gaatttgaag
      181 tcaccttttt acttatgtca cagtctcatt cagtaatgcg ggataaggct ccttattccg
      241 ttatttgaat actctgaagt ttaatctaag aaagggtaat attttgaaaa catgcttcat
      301 cgcatagaaa tgaacttaat ttcagaaaga atacatacat atctttttat atgttttcag
      361 ctgctgaatg gttaaaaagc acaaatatcc gaaaaaaaca tgaagaaatg tacttttttt
      421 gttgtaatgt tcatgaaatt ctctaagtaa attagttaac ataaggttag taagattcct
      481 attctgtgaa taatacgtat cagatttatc ttttattctt actaggaaag taattattaa
      541 gggagttgat ttgaataatg gttagcaaga ataataagag agtatttcta gaaaaaacaa
      601 agaaacgagt attgaagtac agtatcaaaa aattaagtgt tggggttgca tctgttttga
      661 ttggtgttgg tcttgtcttg ggaacaactg aactagttaa agcacaagat gaaatttctc
      721 caagcagccc acttgaaaca gcgataagct ccgtacaagt aggtgacaaa gtagcttcgg
      781 ggaatacttt tcaggagaat cctggttata cgaaaaatta taacttttcg gatttacaat
      841 ttagtcctca agaactaact ggagacacac taaaaggcaa tacaattgga tttgaagttt
      901 acggaaaaca taatattgca gcatcaacta aaaactggga aatccgtctt caattagatg
      961 aacgattagc taaatatgta gaaaaaattc aggtcgatcc aaagaaagga attggatctt
     1021 ctagacggac ctttgtaaga attaatgatt cgctgggtag acctacaaat atttggaagg
     1081 tcaattatat tcgtgcaagt gatggattat ttgctggggc agaaacaacc gatacgcaaa
     1141 ccgctcctaa tggggtaatt acgtttgaaa agagcctaga tgaaattttt aaagagatag
     1201 gcatagacaa tctcaagacc gatcgtttaa tgtatcgaat ctacttggta agtcatcaag
     1261 acgatgataa aattgtaccg ggtattgata gtacgggcta ctttttaaca gattcagatg
     1321 atttctataa tagcttagat gtatctgaaa ataatcctga ccagtttaaa catggatcag
     1381 taagtgcaaa atatgagaaa cctaatactc aaacaaaaga tggttcagga tccacaggtg
     1441 ctaatggagc tataatctta gatcataagt taacgaaaaa ttataatttc tcatattcat
     1501 cttccgcaaa gggcacgcct tggtatgcta attacaaaat tgatgaacga ttagttccat
     1561 atgtagcggg tatacagatg cacatggttc aggctgacaa agtaacatat gatgtttcgt
     1621 ttgaatccgg aacaaaagta gctgatttag caatcgagcg acgggaaggt catgaaaatt
     1681 atggcatagg ttcaataact aataatgatt taactaaact tatcgatttt gctaatgcaa
     1741 gtccacgtcc agtcgttatt cgatatgttt tacaattgac taagccatta gatgaaatct
     1801 tagaagatat gaaagcaaca gcacaagttg aagaaaataa accatttggt gaagatttca
     1861 tctttgattc ttggttgtcg gatacgaata aaaaattaat ccagaacact tatggaacag
     1921 gttattatta tttgcaagat attgatggtg atggaaaccc tgacgataaa gaagagagcg
     1981 gagacacgaa tccatatatc gggaaacctg aattagaaga agtatatgat gttgacacaa
     2041 cagttaaggg gaaagtattc atccacgagt tagcgggaac aggtcacaaa gcccaacttg
     2101 ttgataaaga aggtactgta ttagcagaaa aaactatcgc tccaaatgaa aaagatgggg
     2161 ctccaatttc agatactgta gaatttgaat ttacgggtgt agattcaagt aaattaatcg
     2221 cgaaagatga attaaaaatc caaatcgttt ctccaggttt tgataaacca gaagaaggtt
     2281 caaccgttat taaggaatca ccaaaagcgg ttgataaaca aaccgtggta gttggattta
     2341 aaccagatgc taaagaatca attcggaata ataaaaactt acctgaagat gcagagtatt
     2401 catggaaaac agagcctgat acttctaacg ttactgatag tacgaaaggt attgtaactg
     2461 ttaagatcgg aaatcgaact ttcgacgtgg atgtagagtt tgctgtaaaa gcttctcaag
     2521 ctatggaaaa tgatgcaaca tacgtaccta taacaacaac tccagaaacg acagttcaaa
     2581 gtggaaaacc tacatttgat aaaccagatg ttcctttagc taaagatgcc ttttcaattt
     2641 cagatgtcta taataagtac ttcggtaatg caagtgttga tgcaaacact ggtattgtta
     2701 cattcactcc agctaaaggt gtaggagaat cggagccgat tactggaaca attcctatta
     2761 aaattgttta ccaagatggt tctgtaggca cgaccgattt agcagtaact gtaagtaaag
     2821 atatttatga aaatccagga gaaaacattc ctgcaggcta ccacaaagta accttcaccg
     2881 cagg
//
LOCUS       DAYYDS010000171         2739 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000171 DAYYDS010000000
VERSION     DAYYDS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.177"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACHA02_002861"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACHA02_002861"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8671122.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DAYYDS010000172         2707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000172 DAYYDS010000000
VERSION     DAYYDS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.178"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..518
                     /locus_tag="ACHA02_002862"
     CDS             174..518
                     /locus_tag="ACHA02_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8671123.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="ACHA02_002863"
     CDS             519..938
                     /locus_tag="ACHA02_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8671124.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="ACHA02_002864"
     CDS             complement(1030..1380)
                     /locus_tag="ACHA02_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671125.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2676)
                     /locus_tag="ACHA02_002865"
                     /pseudo
     CDS             complement(1346..2676)
                     /locus_tag="ACHA02_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
BASE COUNT         1002 a          490 c          523 g          692 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaacta
//
LOCUS       DAYYDS010000173         2649 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000173 DAYYDS010000000
VERSION     DAYYDS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.179"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            561..815
                     /locus_tag="ACHA02_002866"
                     /pseudo
     CDS             561..815
                     /locus_tag="ACHA02_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            885..1397
                     /locus_tag="ACHA02_002867"
     CDS             885..1397
                     /locus_tag="ACHA02_002867"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8671126.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1394..2431
                     /locus_tag="ACHA02_002868"
     CDS             1394..2431
                     /locus_tag="ACHA02_002868"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8671127.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          958 a          476 c          534 g          681 t
ORIGIN      
        1 ttttatgccg agaaaactta ttggttggaa tgggctatgt gttagctaac ttgttagcga
       61 gttggttgga cttgaattgg gattaatccc aagaaagtac caactcaaca acacataaag
      121 ccctgtaggt tccgaccaat aaggaaattg gaataaagca acgaaaggag ttgagtaaaa
      181 tgagtgagaa aacgaagaaa atcattcgag tatcattagc tttttcacaa gtagtagtgc
      241 aatcattgat agtttattat acttaccagc aatataaatt atctaaagag aattaagttt
      301 tgattatgta ataagttttg gtactaaaaa agacttgatc taattagacc aagtcttttg
      361 ttagtgttat attaataaca aaataaaaat aagaagtcgc tcactccccg accaaagttt
      421 gtgagcaaaa taaacagtcg atgtttgttt cttaattgta tatcttgata ttaaacgatt
      481 taagattatt cttcaagata tatattcgca aatgtaattg gaaaagcgac ttcttaaaca
      541 aaattaagga gtcgcttttt atggcaaaaa taacgaaaaa tgagcaagaa aatttggtgg
      601 aaaattacac aaaaaaaccg gcgtactcta atagccggtt agacgcacat ttcgtgtgca
      661 cttctaatcc gaaattgagc tttgacgcta tgacgatagt cggaaacctt aataaaaaca
      721 gcgctaaaaa gctatccgat tttatgagtt tagatccaca aattagactt tgggatatac
      781 ttcaaacaaa atttaaagct aaagcactac aagaccccct atgttaggct agttgtcgta
      841 taggataata agttataata aagactatac ggaggatgat taccatgaca aataaaaaca
      901 atgaacgtta ctctaccgaa ctgaaagcga cagccatcaa gaaaatgatg ccacccgaaa
      961 atagggggat tcgccaatta agtcaaactt tgggcattcc agaagcgact ctttattctt
     1021 ggcgtaaaaa agctagaatt caagggaata ctacacccgg aacgaaaaaa aatgtcgaaa
     1081 aatggtcaag tgaagataaa tttttaattg tgatggaatc attctcaatg aaccaagtag
     1141 aacttgcgga atattgccga aagaagggct tatttaaaga acaaattgag gcttggaaga
     1201 aaacgtgctt atctgcgaat gaacaggaag aaaatcgaac gagagaactt gcaactgaac
     1261 taaaagaaga gaaaaaacaa gcgcgtcaat tagagaaaga attacgtcat aaggaaaaag
     1321 cattagcgga agccgctgct ctcttattac taagaaaaaa agcacacgcg atttgggggg
     1381 accaagagga agaatgattt catcttcaga tcgcaaacaa gctgttgaac ttattcaaga
     1441 agccaatcgc aatggggccc gtctcaccta tgcctgtaat gagctgaata ttagcgttcg
     1501 aacctacgaa cgatggacgc gtgaaggtac gatagcgcat gatcaacgcc ctttggcgaa
     1561 acgtcctgta cccaaaaata aactgacgga tcaagaacgc gaaaaaataa tagaaaccgt
     1621 ttcaaaaaaa gaatttatga atttgccacc tagccaaatt gtaccaaaac ttgcggattg
     1681 ctcgatttat attgcatcag agtctagctt ctatcgtgta ttgcgagaaa aaaatatgca
     1741 acatcaccgt ggccgaagtc aagtttcaca aaaacgcata ccgccaagtc accttgcgac
     1801 gaagccaaat gaagtttgga cttgggatat tacttggcta aaaggtccaa taaaaggact
     1861 cttttaccgg ttatatctca tcattgattt atttagccgt aaaatggtag gctgggagat
     1921 ttgggaatca gaagaagctc gtcatgccga aaaacttatt aaacagacgg tgcttaagga
     1981 acgtatccaa ggacagccgt tggttttaca ctccgataat ggcagcccta tgaaagccgc
     2041 gacttttcaa gtcttactcg aaaaattagg aattcaaagt tcttattcaa gaccacgagt
     2101 gagtaatgac aatccttatt cggaagcggc tttccgaacg ttaaaatatc gaccagaata
     2161 tcccaataac ggctttaaaa caatagatga tgctcgaaaa tggacgaaag agtttgtcga
     2221 ttggtataac gattctcatt tgcatagtgg gattcaattt gtgtctcctg cagcgtgtca
     2281 tgaaggacga catctcgcta ttttagagaa acgaaagcag gtttacgagg acgctaaaaa
     2341 gcgccatcct gaacgctggt caaaaggtgt tcgaaattgg actccacatc aatcggtggc
     2401 gttgaatcct ttaaaagaaa cagaaagtta acaggagcat aatctttcaa gattatcaaa
     2461 taaccctcgt cacccaatag gggaaaaagt tattcttccc ctattggata acgaggccaa
     2521 ccgaccatag ggaggttctg ctttttgata ggtggaaaat aaaaatacga caactatatt
     2581 gacaaacacc gaccatcgtc cagtaaagag acgcaataaa ttctatcgaa gtttacgcac
     2641 tgcctctac
//
LOCUS       DAYYDS010000174         2640 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000174 DAYYDS010000000
VERSION     DAYYDS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.180"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACHA02_002869"
     CDS             complement(<1..153)
                     /locus_tag="ACHA02_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8671128.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="ACHA02_002870"
     CDS             complement(475..696)
                     /locus_tag="ACHA02_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671129.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="ACHA02_002871"
     CDS             complement(794..1039)
                     /locus_tag="ACHA02_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671130.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="ACHA02_002872"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="ACHA02_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2263..>2640)
                     /locus_tag="ACHA02_002873"
     CDS             complement(2263..>2640)
                     /locus_tag="ACHA02_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8671131.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          889 a          465 c          362 g          924 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     2281 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac
     2341 agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat
     2401 caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc
     2461 cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt
     2521 gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga
     2581 attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg
//
LOCUS       DAYYDS010000175         2634 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000175 DAYYDS010000000
VERSION     DAYYDS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.181"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(261..545)
                     /locus_tag="ACHA02_002874"
     CDS             complement(261..545)
                     /locus_tag="ACHA02_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcQ family protein"
                     /protein_id="HFM8671132.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
     gene            complement(566..1969)
                     /locus_tag="ACHA02_002875"
     CDS             complement(566..1969)
                     /locus_tag="ACHA02_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HFM8671133.1"
                     /translation="MTASDLIRNFIMMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDF
                     FRYYLAAITGEYSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPE
                     GKYSEVLSDFQSMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNG
                     DDTSRVSKAFPSYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNL
                     LNANAYAMRLARWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYT
                     GLVNTIGNLTLVTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISAR
                     SEALINELITMYPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYS
                     KIKDIASDSLDETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDY
                     WKYDNGISINDSLRNKK"
     gene            2155..>2634
                     /locus_tag="ACHA02_002876"
     CDS             2155..>2634
                     /locus_tag="ACHA02_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8671134.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSQLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     "
BASE COUNT          817 a          536 c          392 g          889 t
ORIGIN      
        1 tatgtgaaaa tcgaatcttg agatagctat tccccttgag atattcactg atacttctac
       61 tcaaatcatt taaattttta taattatttg tacggaacgt atatatatca acatctattt
      121 agacgttgtc ggtgatacaa tgtcattttt cccgtgtact atgtcattta tctctatact
      181 ttgtcatttt tctttttggg caatcaaaaa aggcaacaaa ccaattatga tttgctacct
      241 tctccgttta aaaacttatc ttagccccaa aatggaatcc aatttttaac ttcttttgcc
      301 aatgcatacg ccttagcaag acctgagttt tcaactaaat atttttcccc ttccagagaa
      361 acactccccc agatatgaac agtgttatcc gcccaaacca tggattctag taagccattg
      421 cgcgaaagaa atttagtttg ttctaaaata tcttcttctg gaacttgccc tttaagctca
      481 ataaaaggat tctttttttc atcacgcaat ttaagaattg catatcttag tttgttctta
      541 tccaacctaa caccacctct atgctctatt tcttatttct tagcgaatca ttaatactta
      601 ttccgttatc atatttccag tagtcccaac cattccggct accccctaat agcagagctg
      661 ctgcattcga aacagatgag gcagtgtaat cttgaacaaa ttgtagtccc ccaattgttt
      721 cttcaatgat tccattatcc aacagctctt ggcgagtttc atctagcgaa tcagatgcaa
      781 tatctttaat cttagaataa atttctgaac ctgaatgtat aatcacggtc tcgtcttcgt
      841 tcaaataccc agttgctttt attccttgcg cctctaagaa aatttcccta ttcccatcgt
      901 gttcatagtc gcccgacgat ctcaaatagg gatacatagt aatcagttca ttgattagag
      961 cttcacttct ggcagaaata tccgatgatg tccactcagt caattcgtac aaatctttat
     1021 tcatacgaat atgaagggta tcttcaaaga ttttcttttt ggtctcaaag tctttatttc
     1081 ccgcggccga attatcagga tttgtaacga gtgttaaatt accaatggta tttactaatc
     1141 ctgtgtattc ttcccccgat gtaccagcct tctcttccca atatgaagta ctagtctgtg
     1201 gcatgatgtg ttcaatggac aagttactca tatcaagtgt tgctgaattt tctcgattct
     1261 ctattttttc aagtaaccaa cgggccaatc tcatcgcata agcattggca ttcaatagat
     1321 tagatttcaa tgccttgtca gatggaagtg ccatattatt tgattgattt ctagtaacaa
     1381 gaacataaat tacaatatca acgatatttt catatccatt agctttagcg tacttcctaa
     1441 cacttttcaa atatgaagga aatgcttttg acactctgct tgtatcatcc ccattaaaat
     1501 atctcctaag ctgataatca ttcactacct taatcgcatc aatatattgg tcttcagtga
     1561 taaattggtc atgatagtac cattcagata atcccaaaac aaaaggtgca ggcatcatac
     1621 tttccatact ttggaaatcg ctcaatactt ctgagtattt tccttcaggt ttgttatagt
     1681 aaagtctcgc aaaataacca gcatatcgca ctaatttttg gagcaattcg gcatcacttg
     1741 atacatcgcg ctctttatgc caatagtctt tgaatgcttg ataaagaata tgctttgctg
     1801 aatactcccc agttattgca gcaaggtagt atctgaaaaa gtcttccatt cctttagaat
     1861 tatcaaagac ttcttccaac ctacgccaat atttagtata aagcgtagtc tgctcttcat
     1921 tagatttatc catcatgatg aagtttcgaa tcaaatccga ggcagtcaat ctttcaccgg
     1981 tagaattgat actttcaaaa atttgctgtg cattatcacg gtcatctaat ttaatataaa
     2041 caatacgcgt ggtgctagtt aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa
     2101 tcaacaaaaa acgcatatag tagtctcaaa actcccaaga aatgaggact acatatgcgt
     2161 tcatcaaaat atactgaaca ttatacagat acttttataa cattcaagga aattatgaga
     2221 actgtttcta acatatacca caattgtgta ccagataaga ttaaaaaccg aagaaatact
     2281 gatcaactga agcaacgcga tacagtgatt attgcttgtg ttatatgggg cataattaat
     2341 ggctatacta gccaaagagc cacgtataga gcggtttgtt ccgtcttatt tcttaatggt
     2401 gactttccta gtaggagtcg attcactcgc ttaagttcaa acttagctta cactatcaag
     2461 attattcgat actttttcat caagaagctc accaaaggtg aactagtcgg aattatagat
     2521 agttttccta gtcaattatg taaaccagtt agaaatagac aagcaaaact attgaatcaa
     2581 atagccaaag ttggctataa ctcaacaaaa aaatcttatt tttatggtct taaa
//
LOCUS       DAYYDS010000176         2530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000176 DAYYDS010000000
VERSION     DAYYDS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.182"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACHA02_002877"
     CDS             <1..1412
                     /locus_tag="ACHA02_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8671135.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2530
                     /locus_tag="ACHA02_002878"
     CDS             1546..>2530
                     /locus_tag="ACHA02_002878"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8671136.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTN"
BASE COUNT          997 a          354 c          512 g          667 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg
//
LOCUS       DAYYDS010000177         2473 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000177 DAYYDS010000000
VERSION     DAYYDS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.183"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACHA02_002879"
     CDS             85..624
                     /locus_tag="ACHA02_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671137.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            1644..>2473
                     /locus_tag="ACHA02_002880"
     CDS             1644..>2473
                     /locus_tag="ACHA02_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8671138.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          905 a          326 c          397 g          845 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt tta
//
LOCUS       DAYYDS010000178         2415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000178 DAYYDS010000000
VERSION     DAYYDS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.184"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..2268
                     /locus_tag="ACHA02_002881"
     CDS             115..2268
                     /locus_tag="ACHA02_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8671139.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          823 a          425 c          535 g          632 t
ORIGIN      
        1 tgattttatc ttgaaattag gtaaagaatt tcttgctccg cccatttttt cttttttatt
       61 aagaagtttt tgggtacaat gaagcatgaa tgtaaatagg cggaaggata ggaaatgatg
      121 aaagatttta tgaaaaaaat attaaatact tcttggttag aaaaaataag aaaaggtgca
      181 caagaaccta agaacaaaac gtttcgccgg tcccatgttc cttttcgctt aaattttctt
      241 tttttcatta tatttacgtt atttgttgcg ttgattgcta gattgggtta tttgcagatc
      301 gtgaacggcg aagagatgga ggcgcgagtg aagtctactt ctacgattac ggtccaagaa
      361 agtactcctc gcggaatgat ctacgactcc acaggaaaag ctttagttac gaataaagcg
      421 aatcaggcca tcacatatac gagaggaaca caagttacgg ctaaagattt gcttgaaatc
      481 gcaacgaagc tgaactcgct gatcgatgtt ccggtagatg aaaatctgac agatagagat
      541 aaaaaagatt tttggctggc tgatcccaaa catttgaaag aagccacaaa aagactttcg
      601 gcaaaagaaa agcaattagg gaacagcgaa caatatagtg cgatggtcaa taaagtcaca
      661 gctgatgaga ttaatttaaa cgaggagcaa ttaaaaattg ctaccatctt taaacggatg
      721 aatagtgcat atgaattaaa cactgtctat atcaaaaact ctggtgttac tgatcaagag
      781 ttagctgttg tggcagaacg cgcttctgaa cttccaggag tttcgactgg cacagactgg
      841 acacgagaat ataatgcagc aagttcactg aaaagtattc ttggcagtgt gacgactgaa
      901 aagcaaggac ttccagcaga tgaagcagag aaatacctag caaaaggtta ttctcggaat
      961 gaccgtgtag gacaaagtta tttggaaaaa caatatgaag atgtcctgca aggaaccaaa
     1021 acccaatatg aagtttccct tgataatgaa ggaaatgttt cgaatcaaaa agaaatcttc
     1081 tctggggaaa aaggttctaa tttaatgtta tctatgaatg cagaattcca atcaaaagta
     1141 gaagaaattt tgaaaaggaa ttatcaaaca ttgatcaaca acggcaaagc acagtattcg
     1201 cctggtgctt atgcagtggc tatgaatcct cagacaggag aagtgctagc aatgactggt
     1261 ttcagtcatg aacaaggttc caaagaaatc accgagaatg ctttaggcac gattactagc
     1321 gcctttgatc cagggtctgt agtcaaggct ggtactttga cagcaggctg ggcaagtaac
     1381 gcgatcagcg gaaatcaagt tttgattgat gagccgattc gtctgcaagg tgcttcagaa
     1441 aaaagctctg tctttaatcg aagcggacaa gttgctcttg atgcagtcaa agcatcggaa
     1501 ttgtcttcta atacgtatat gatcaaagtg gcgttaaaga tgttaggact agactataca
     1561 cctggaatgg gactacctag tttagatgaa gaagctaaag cttatcagca attgagagac
     1621 tctttcaaag agtttggtct tggtacaacg actgggatcg atttgccgaa tgaatctcct
     1681 gggatctcac gttctgttga ttatatgaag aagttcaatg cggataatgg caaagaatgg
     1741 tatgctccag gtaactttac cgatttggca tttggacagt ttgacacata tacaccaatc
     1801 caattagctc aatatgcttc aacagtcgct aatggcggga aacgagtaca gccgcaccta
     1861 gtcaaagcga tctatggcaa tgacgaaaat ggaaacttgg gtgaagtgaa gaaagaaatt
     1921 ggcacaacag tagaaaatac ggtcaatatt tctgcagaaa atatggcgat cctccgtgaa
     1981 ggattccacc aagttgtcca tggtaccgat ccttacacaa cagccaaacc attagccagt
     2041 gcgaaaatgg acctttccgc aaaaacaggg actgcagaaa cggttgcgga aggtcatcca
     2101 gatattgcga cagtcaatag taatatcgtg gcatacggac caacagacaa tccggaaatt
     2161 gccatcagtg ttgtcttgcc gaacttatta gacgaacaag accacataaa cttgacgatt
     2221 gctaaagaaa taatggacgc gtattatgat atgttcatgg caaaataaaa aagaggtcgt
     2281 gaaaaagccg cttagtttcg agtatgaaga aaaattttgt gaaaatagct tctcacattt
     2341 ttgcttaaat ttgacttcat accgagaaac tggcttttga acaccgttta ctcggcaaaa
     2401 aaggttgtga caaaa
//
LOCUS       DAYYDS010000179         2407 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000179 DAYYDS010000000
VERSION     DAYYDS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.185"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACHA02_002882"
     CDS             5..1150
                     /locus_tag="ACHA02_002882"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8671140.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACHA02_002883"
     CDS             1321..1779
                     /locus_tag="ACHA02_002883"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8671141.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ACHA02_002884"
     CDS             1938..>2407
                     /locus_tag="ACHA02_002884"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8671142.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAYYDS010000180         2398 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000180 DAYYDS010000000
VERSION     DAYYDS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.186"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHA02_002885"
     CDS             207..2069
                     /locus_tag="ACHA02_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8671143.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACHA02_002886"
     CDS             2069..>2398
                     /locus_tag="ACHA02_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8671144.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAYYDS010000181         2145 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000181 DAYYDS010000000
VERSION     DAYYDS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.187"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1057
                     /locus_tag="ACHA02_002887"
     CDS             29..1057
                     /locus_tag="ACHA02_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM8671145.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1113..1331
                     /locus_tag="ACHA02_002888"
     CDS             1113..1331
                     /locus_tag="ACHA02_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671146.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1402..>2145
                     /locus_tag="ACHA02_002889"
     CDS             1402..>2145
                     /locus_tag="ACHA02_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671147.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSH"
BASE COUNT          822 a          246 c          381 g          696 t
ORIGIN      
        1 ttatatttaa ataaggagta tgataataat gaatagcaaa aaacttcaga tgggtgaaat
       61 tgttagaggt cctagtcaaa tatctattga tgttacatat aaatgtaatt ttagatgtaa
      121 acattgttac aatgcgagtg gtggagacaa ttgttttatg gataatgagt tgactgatga
      181 tgaactgtta gaacttacaa aagatatatg taatttaaag ccatataata tttgtttctg
      241 tggaggagaa actttactga gaaaaaaaat agttttatct tgtgccactt tattaaaaga
      301 ggcaggtatt cctaatattg ctattgttac taatggatca ttagtaactc aggatgttgc
      361 aaaagaatta ttcaaagcag gtattaatcg atttcaagta agtttagatg gaaaaaatgc
      421 tagtagccat gaagcactta gattatatcc aaacagtttt gaaaaggcaa ttaatgctat
      481 caaattattt agagaattgt ccgaagaagt ggatgttgct ttttgtccta ctagttttaa
      541 ttacaatcaa ttacgagaag tatatcaact agtaaatact cttaaagtga agtctttaag
      601 aattcaacca tttatgctta gtggtagggg aatgcaaaat agggaaatag tgccaactgt
      661 ttctcaatat agaaaactag tttctgatat taactatatt aaaagaaatg aagattatac
      721 caagatagat tggggagatc caattgatca tatcatccga cacagaactt tattgaaaga
      781 tttttcgaat tttgtttcta ttaggtcaga cggaggatta atggcatcac cttacttgcc
      841 aattcaagtt ggaaatatta aatgtcacag tctttctaaa tattggcgag caggacttta
      901 taaagttttt tctttagatt tagttcaaga tatagctact caagtttctt caatacaaga
      961 tatgagtaaa gaattaaaaa aaggacatat taaatggctt aatgaagata tagaaattga
     1021 tattctcgat gaatacaaac atgatatttt aatttaggtt tatcattgga aagtttagat
     1081 aaacaaagta cttttagaaa ggagggaaaa aaatgatttc tgaacctcaa atgaataata
     1141 tgtatgaaag cccaactata atgggagctg gtgaagctgc gccaagagct tgttcagcaa
     1201 ttatggtgtg tgtgttatta gcatctgcag gagtaggtat ccaagcagga gcggtgtata
     1261 cttatgctgt agttgctaca gtagctgtag ctgcagccgc agtttggaaa acagtaggat
     1321 tagttggtta ggaattaata ggagactaac tgtctcctat tgattccaat gattaagcta
     1381 aggaggaatt ttaagaaaat aatgaaatac aataatttaa aaccgaaatt acttaatgag
     1441 aacatagaat atataagaga agaaaattat ggatatttaa ccatagttcc caaaaatgga
     1501 actgaattga aagataacgt tttcaatcca acagtaagat atatattggc taactgtact
     1561 ggaaaaaaca gtgtaaaaaa aatagcgaat ggtttaaaaa agaagtttcc tagtttagaa
     1621 tatgaaaaat tctatcaaga tgttgtagga actattaaat ttttcaaaca ctataatatt
     1681 attgatatag gagattatag tatggaagaa aagaaaatat ataaaattga acaaaattta
     1741 gaattagaat tactaaatga atcacattat gaagaaattt ataattttat cacagatcat
     1801 cctgaaagta tgtattttca tgaagatatt tatcatcaag gaaatagtaa agaattagaa
     1861 gttgtcctta gatataattt attttcgttt acgagtgaat tttacttttt aagacataaa
     1921 gagagaattg taggaatggt ttctataatt gttaatccat ttcaaaactt atcctacagt
     1981 atagtaaatt acctcatatg tgagcctgca tatactacga ctattttgac aacagtatta
     2041 gaaaatatta acgaagtttc aattttagaa gtaaagaagc tagtgattca tttgctaaat
     2101 tcaacagaag atgaatcact aatcccctat ttaaaagatt cacat
//
LOCUS       DAYYDS010000182         2065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000182 DAYYDS010000000
VERSION     DAYYDS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.189"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1008
                     /locus_tag="ACHA02_002890"
     CDS             <1..1008
                     /locus_tag="ACHA02_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671148.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ACHA02_002891"
     CDS             1025..1174
                     /locus_tag="ACHA02_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671149.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ACHA02_002892"
     CDS             1171..1617
                     /locus_tag="ACHA02_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671150.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ACHA02_002893"
     CDS             1619..1756
                     /locus_tag="ACHA02_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8671151.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="ACHA02_002894"
     CDS             1794..>2065
                     /locus_tag="ACHA02_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671152.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DAYYDS010000183         2058 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000183 DAYYDS010000000
VERSION     DAYYDS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.190"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..337)
                     /locus_tag="ACHA02_002895"
     CDS             complement(<1..337)
                     /locus_tag="ACHA02_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8671153.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="ACHA02_002896"
     CDS             complement(404..559)
                     /locus_tag="ACHA02_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFM8671154.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="ACHA02_002897"
     CDS             1176..1397
                     /locus_tag="ACHA02_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671155.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="ACHA02_002898"
     CDS             1398..1655
                     /locus_tag="ACHA02_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671156.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="ACHA02_002899"
     CDS             complement(1791..>2058)
                     /locus_tag="ACHA02_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005727 - extrachromosomal circular DNA
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rep family protein"
                     /protein_id="HFM8671157.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DAYYDS010000184         2040 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000184 DAYYDS010000000
VERSION     DAYYDS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.191"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1872
                     /locus_tag="ACHA02_002900"
     CDS             <1..1872
                     /locus_tag="ACHA02_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8671158.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          738 a          330 c          416 g          556 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DAYYDS010000185         2036 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000185 DAYYDS010000000
VERSION     DAYYDS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.192"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..304
                     /locus_tag="ACHA02_002901"
     CDS             <1..304
                     /locus_tag="ACHA02_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8671159.1"
                     /translation="KKFSLYPEKFFLVKDLWIAVMQNSSNKLPKTYNHIAFKIDEQDI
                     DSFVSKIQMLGLTVEPGRSRVKGEASSIYFYDYDNHLFELHTGTLQERLKSYNSTT"
     gene            complement(576..866)
                     /locus_tag="ACHA02_002902"
     CDS             complement(576..866)
                     /locus_tag="ACHA02_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671160.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1074..1766)
                     /locus_tag="ACHA02_002903"
     CDS             complement(1074..1766)
                     /locus_tag="ACHA02_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8671161.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          567 a          381 c          304 g          784 t
ORIGIN      
        1 aaaaaaattt tcattatatc cagaaaaatt tttcttagtt aaggacctat ggattgcagt
       61 aatgcagaat tcgtcgaata aactgccaaa gacatacaat catattgctt ttaaaattga
      121 cgaacaagat atagattcgt ttgtctcaaa aatccaaatg ttaggtttaa ctgtcgaacc
      181 aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac ttttacgact atgataatca
      241 cctcttcgag ttacataccg gtactctcca ggaacgttta aaatcctaca attccactac
      301 ctgagactag taatgactag tctcttttta tggcctctga tccccccatt gagcgaatac
      361 taatagggct tcgaattgat ctgttcaatc tttaagcaat atccctcaaa tgagtttgga
      421 aagtttagtg attcatcaca aatacttaaa tacatgtagt tatatataga ttgaggttct
      481 tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg catccatctt tccaaaacaa
      541 gcggctagga catttttgtc tcagccgctt gttttctagt ttattctttc atcttgtcgg
      601 accttttttc ttgggtatag acttttctca tattctacca caattttggg tttccctaac
      661 acatgatagg atctcacaac aaattgcttt ctattattat aataacctgt aacgactaac
      721 ttcatccctt cgtctacttc agctaggaag ttcaaactat gggaagcaat caaacaattg
      781 accccattta aactgaaacg gatcaacggt ctggcactaa cttgcaaagt acggatctta
      841 tctactgttc catatttttt ctccataacc ttcacctcaa taccattata cgaacataca
      901 ttccctttgt aaagaagaaa ctctgtttta tctttttgac tttttgtcta ttacctttct
      961 taaaatcata ttggcacttc ctgcgaagat gaaggacatg tactaaaaaa aagaggcagc
     1021 agtatagatt actgttgctt ctttactaat ttaggtttac ttgttaagaa agattattta
     1081 cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg tatcttccac ttcatctttt
     1141 gctttttcta acttttgaat atattgtttc aagcttgagt tttcagcagt tgcttcgtct
     1201 aatttctttt gtgtatctgt taactgagtt tctacatttg ttttttcttg tgtcagtgtt
     1261 gcgatttgtt gattcaaacc atttacagta ttttgtaatt ctgaaagttt tgcttgtgct
     1321 tcttgtagtc ttgctgtcca atctgcattc agatgattga tttcatcatt tttttggttg
     1381 atctcatcta gtttgacatt gatttcattt tgtttgcttt gtagttgtgc atccttgctg
     1441 actaattgac tttctaagtt ttgtttttct gctgccgatt gatttttttg gttttctaat
     1501 tcatttactt ttgtttggta ttcttggaca tctgcctttg aatcttgcaa tgtctgttct
     1561 ttttgtgcta gtcgagcggt aagatctgta agcgcttgct ctttatctgt caatgtatga
     1621 tcaagctgtg tcagattctc attgattgta tcaatatctg tctctccacc ccacaaaata
     1681 atttcatttg cgtaccgcgt gcctgtgtag ccaattagac atagaaatgc aacgacaatc
     1741 ccaccagtaa taaatttctt tttcatttgc atagctcctt aatccaatac ttttacatgc
     1801 taaaagtaag tgatctcaaa cacaatgtca agcgttttta aattacatgt gtaattttaa
     1861 tataaatact atcaaatact catttaatcc tcctttttta tctatttaaa aaatcttttt
     1921 tatagagaaa aatgcaggtt aatactttct taaataaata tttatggtga acaaaaacaa
     1981 aaaagcaagt tttattaaac gtaacccttt tcatttcata gattactgta tttatt
//
LOCUS       DAYYDS010000186         2003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000186 DAYYDS010000000
VERSION     DAYYDS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.193"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACHA02_002904"
     CDS             <1..378
                     /locus_tag="ACHA02_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8671162.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACHA02_002905"
     CDS             426..1826
                     /locus_tag="ACHA02_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8671163.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAYYDS010000187         1993 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000187 DAYYDS010000000
VERSION     DAYYDS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.194"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..644
                     /locus_tag="ACHA02_002906"
     CDS             135..644
                     /locus_tag="ACHA02_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8671164.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            737..1899
                     /locus_tag="ACHA02_002907"
     CDS             join(737..1013,1013..1899)
                     /locus_tag="ACHA02_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8671165.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
BASE COUNT          698 a          353 c          395 g          547 t
ORIGIN      
        1 tgtaatcatt gataagtcca gtgttttagc ggataatcag ctttttattt attttttctt
       61 ataaatttga taaatagaaa ttagtatagc cctaaacaaa ttatttgtgt tttattgttt
      121 agggctatac tatattggta caaggaatag aaagaggtgt ttatgtggtt gataaaattc
      181 agtttaatca agttatagaa gaattcatca aagtaaataa tttaatgacc catattcaaa
      241 aacgaccaat cagaataact aatgatctaa aactcagtac gagtatgatt catttgattg
      301 atgtgattgg aaattatcct gaaactagta ttacggaatt agctgatcga ttaggcgtta
      361 ccaaaggagc tgtatcccaa cagattccca ctttagagaa aatgaatctt ataagcattt
      421 ctcaaaaaaa aggaaataag aaaactaaac ttgtatcgtt aacaaaatat ggtcgaaagg
      481 tgtacgaatc acataattat ttacatgaag agctttatag ctcaataaat gaagcgttag
      541 aatcattctc accagaccaa atttcaacca ttaaacagat tctagaaaaa gtatcttata
      601 caatcaatga atatcaagta agtctgacaa gaaaggaaga ttgacatgaa aaaaatgatt
      661 tttgaatcgg ctacactaga ctagacagaa aaaataaggt gtgtaaaata aatgaaaaca
      721 cactggagga atttttatgt caagacgtca acgtagaacc tattcaaaag aattcaaaca
      781 acaagtcgtc gatctttatc tcgcaggaaa gccccgagct gaaatcattc gagattatga
      841 gctgactacc tcggcctttg ataagtgggt caagcaagcg caaactactg gctcgtttca
      901 ggaaaaagat aacttgatcc ctgaacaagt agaactaatt gcattacgaa agaaaaataa
      961 acagcttgaa atggagaatg acattttaaa gcaagcggcg ctgatattgg gacgaaaaga
     1021 caagtaattg ataccaataa gcacaaatac tcaatatcag cgatgtgtaa agtgttaaac
     1081 atctcttgcc aaacctatta ttatcaagcc aaaccagttg aagatgaatc agaactggaa
     1141 gaactcgttc aagaagaatt tattcgcaat cgaaaagctt atggtaccag gaagctgaag
     1201 cagtgcttgg ccaatctagg tcttcaagtg agtcaacgca gaatcggccg tatgatgaaa
     1261 cagcgtggct tgcgttcaac atacacattg gctcatttca aattccattc ttctacttgt
     1321 aacgaagcca agaccgccaa tattctagac ggaaccttcc tacaggaaaa gtcattagaa
     1381 gcgattgtaa ccgaccttac ctatgttcgt gttgggaaga aatggcagta tatctgcctg
     1441 attcttgatt tgtttaatcg agaaatcatc ggctattcat gcggggagaa gaaagatgcg
     1501 acgttagtca aggaagcttt tgcaaagatt ccgtactcgt tgaataatgt acaacttttt
     1561 cacacggatc gtggaaaaga attcgataac cagacgattg acgaaatttt atgggccttt
     1621 aatatcacgc gttccttaag caaaaaaggc tgcccctatg ataacgccgt tgcagaatcc
     1681 acctataaat ctgtcaaagt agaattcgtt catcaatacc gctttgagac attggctaat
     1741 ttgcgattag agctatttga ttacgtccat tggtggaata ccatccggct gcacggcacg
     1801 ttaggctatg agacaccgat cagttttcga cagcagagat tggcgcagcg aatccttgat
     1861 aatgagctcg gatgtgatac cggtggcgag gcaccttaat tatttgaatg agcttctgcc
     1921 ttagagaatc agattcgagg aggctcgttg tcaagggcag tcggaacacc agcagcaagc
     1981 ttacgcaata cct
//
LOCUS       DAYYDS010000188         1992 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000188 DAYYDS010000000
VERSION     DAYYDS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.195"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACHA02_002908"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACHA02_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8671166.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACHA02_002909"
     CDS             852..1040
                     /locus_tag="ACHA02_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8671167.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACHA02_002910"
     CDS             1053..1610
                     /locus_tag="ACHA02_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8671168.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACHA02_002911"
     CDS             1642..1884
                     /locus_tag="ACHA02_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8671169.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          554 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ct
//
LOCUS       DAYYDS010000189         1962 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000189 DAYYDS010000000
VERSION     DAYYDS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.196"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACHA02_002912"
     CDS             complement(<1..272)
                     /locus_tag="ACHA02_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671170.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="ACHA02_002913"
     CDS             complement(309..446)
                     /locus_tag="ACHA02_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8671171.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="ACHA02_002914"
     CDS             complement(448..894)
                     /locus_tag="ACHA02_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671172.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="ACHA02_002915"
     CDS             complement(891..1040)
                     /locus_tag="ACHA02_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671173.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..>1962)
                     /locus_tag="ACHA02_002916"
     CDS             complement(1054..>1962)
                     /locus_tag="ACHA02_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671174.1"
                     /translation="LLDDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELII
                     GQTYTITLKGTKPASQTFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDL
                     RIFQLPKETAGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYT
                     EKGLNDTVSLTEPQSIEGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKL
                     YREGKTVTIYFFALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSAYSFVITYSI"
BASE COUNT          509 a          407 c          342 g          704 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aa
//
LOCUS       DAYYDS010000190         1848 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000190 DAYYDS010000000
VERSION     DAYYDS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.198"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..712
                     /locus_tag="ACHA02_002917"
     CDS             146..712
                     /locus_tag="ACHA02_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8671175.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            857..944
                     /locus_tag="ACHA02_002918"
     tRNA            857..944
                     /locus_tag="ACHA02_002918"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:889..891,aa:Ser,seq:cga)
     gene            1278..>1848
                     /locus_tag="ACHA02_002919"
     CDS             1278..>1848
                     /locus_tag="ACHA02_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM8671176.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          647 a          277 c          356 g          568 t
ORIGIN      
        1 atttttttaa ttttgcaaaa aaaagttaaa gaagactgaa aaaaaattta aaatataacg
       61 ttttgagtaa aatgtttgca aaaaatgcta aaaatagggt acaataaaac aacatatact
      121 gcagtaaaga agtgggggag ttttaatgag aagaaaagtc tataccaagg atcaaattct
      181 aaaagctgct tatgatgttg ttgcaaagga agggtttaaa ggatttactg cgagaaatat
      241 tgctaaaaag atggggattt cgacacagcc gatttatctg gaattcaaaa acatggaaga
      301 tttaaaaaat acgttgctgg attctatctt tgaacaacta aaagaagatg ttttccctat
      361 tgaacgtact ggtgataaaa tcattgatct cggtttgaac tatatctact ttgcaaaaaa
      421 gcatcatagt ctttacatgg cattgtacat tgatgaacat ggggacggaa agaaaatgca
      481 tgatttttca tacaattatt tccgtgaaat agttaaagaa gatgaaagat acaatcaatt
      541 atcggatgaa tatgtcgatg ctctgcatac tggtacatgg gtagcagtga ctggcttagc
      601 gtcattgatg tcatcaaaca tcatgcatcc aacagaagaa caaatcattg cactgatcaa
      661 acggacaatt gactcgattt tagcattaga taatcctgaa ataatcagat agtgttctat
      721 cagtatgaat ttgatcggta agtccgttgg ctgcaaactg tcaatggact tttttgctaa
      781 aaaaagcgat ttctgttatt tatctattag aaataggctt gacatttcgt gaaaaaagtt
      841 taaaaatagt acttacggaa ggttggcaga gtggtaatgc accggactcg aaatccggcg
      901 aaccggctaa taccggcgcg caggttcaaa tcctgtacct tccttatatc aacgattaga
      961 ggttgtgaag aagctgtcct gcatcaagta ataagaacgg ccaaacaaaa atagcttttc
     1021 ctattttttc gtttgattgg gcttattacc gcagatgcaa gcttttgaac accgtttgtt
     1081 cggaataagg ggctgtgaca agacttttgt cacagcccct tattgttgta cttttaacct
     1141 acaatatttt ttgattcccc ctgagtacta ttttttataa atgctagtaa atcaatcttc
     1201 tttggccttt ttttgataaa aaaaatacta gatgtaagat aaaagtgtaa aaacgatttg
     1261 ttttaggagg ggaaacgatg tgtaccgcaa tcacttatgc aacgaaagac cattattttg
     1321 gaaggaattt tgattatgag atgtcttata atgaagtggt cactattact ccaagaaatt
     1381 atcgtttcga ttttagaaag gtaaaaaatt tagataaaca ttatgcaatg ataggaatag
     1441 cagctggggt atccaactat ccactctatt atgaggctac aaatgaaaaa gggttgagta
     1501 tggcaggact gaattttcct ggcaatgctg attataaaga attacaagaa ggaaaagata
     1561 acgttgcacc atttgagttt attccatgga tactagggca atgctcaacc atagacgaag
     1621 ctaaagaatt attggctact atcaatctag tcaatatcga ttttagtgaa aaactaccct
     1681 tgtcaccttt acactggtta ttagctgata aagaaaaatc gatagtaatt gaaagtatga
     1741 aagatggact tcatttatat gataatcctg tcggtgtgtt gacaaataat ccgccatttg
     1801 attatcaatt atttaatctg aacaattatc gctcgctatc aaatggaa
//
LOCUS       DAYYDS010000191         1800 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000191 DAYYDS010000000
VERSION     DAYYDS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.199"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..1382
                     /gene="brnQ"
                     /locus_tag="ACHA02_002920"
     CDS             30..1382
                     /gene="brnQ"
                     /locus_tag="ACHA02_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8671177.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          310 c          353 g          631 t
ORIGIN      
        1 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
       61 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
      121 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
      181 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
      241 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
      301 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
      361 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
      421 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
      481 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
      541 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
      601 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
      661 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
      721 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
      781 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
      841 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
      901 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
      961 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
     1021 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
     1081 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
     1141 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
     1201 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
     1261 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
     1321 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
     1381 gataaaagag gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa
     1441 tggcttctca catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc
     1501 gtttattgag aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa
     1561 aatggcttct cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca
     1621 ccgtttattg agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc
     1681 tccatatact ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat
     1741 ttcttttata atatagattt tagacatata tatgatataa aaatagtttt cgcaaaaaaa
//
LOCUS       DAYYDS010000192         1717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000192 DAYYDS010000000
VERSION     DAYYDS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.201"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(10..1437)
                     /locus_tag="ACHA02_002921"
     CDS             complement(10..1437)
                     /locus_tag="ACHA02_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8671178.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            complement(1430..>1717)
                     /locus_tag="ACHA02_002922"
     CDS             complement(1430..>1717)
                     /locus_tag="ACHA02_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFM8671179.1"
                     /translation="FHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHII
                     DPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          661 a          282 c          195 g          579 t
ORIGIN      
        1 gtcttcatat taattctcct tttttaaata caagtaagaa atgcttccca aataatacca
       61 gaaaacaatt gaaatgacat cgcgcttatt gaaaagtaca tgagcctcca ccatttcatc
      121 tataaaaata gatagtataa aaattaaaac aataccaaga aacgcataat tcactttact
      181 tctaaaaaat gttttgagta aactaaagat atttaacaca tagaaagcat agatacatat
      241 aaaagcaatt ataccacttg atacaagtac ttgaagaaat ccattatgca tgtttccatg
      301 tgctcttttt agttcattca catctgtagt atcaagatct acttttttca aaaattttgc
      361 atcgctattc ccatagaaat cggtctctcc ataaccaaat aacaaagttg gtcttataac
      421 tttaagcgca gctttccaaa tcgtaaaacg accagctgaa atttcggagt tatcactaga
      481 atgctgtaat attacttctt tttctggaag tggagtttta tctgattcat ttgactctat
      541 aacttctact tttctatcaa tcgatacagg aattgttgct aaaaaatcct tagcataatt
      601 agtaccaaaa gaaaaatata ccaatagaac taataatatg cttaccatcc atttgatttt
      661 tacaggtagt acttctctaa tgctctttct aaattttgaa ttggcaaaca acaaaagaaa
      721 taatactaaa aaagaacaaa ttactacctg ggcacctcta gacgaagaaa gtgtaatata
      781 aaatatttgt acgataaaat tcatcatata aaaaacacga atcgtttttg ttatttttga
      841 aattaataca ctaattaaac ttaaaataat agaagtatat ccaaacatag ctccaacatt
      901 tggacttgta tagacaccaa ataaccgatg ttcaataaaa ccttgtcttg caagtaatcc
      961 tggcgttcct gttgggacat gataagaaat caaaaaaata aattgataga tagatattag
     1021 cgcagctaaa aaaattatga taatgaaaac attattaaaa tacaccattt ttttcttttt
     1081 ttcctcttca ctttgtgaaa agtctgcagg aaaaattact atcaaagtaa tcagcaaata
     1141 tccaaaatta taaatagatg gaattaaaaa attttctctg ttaatgatta tagttaagat
     1201 aaaactaatc attgctccaa tcagtagcca agaaaaacgc attttaaaaa aatttttctt
     1261 tataaaaacc tcatacaaaa ctataattcc accccaagca aaagctattt tcatcgggat
     1321 attagttaca ttaaaagtac taggcataaa ataaagcaga caaatgatta aatttattac
     1381 tcgaaacgca tctcgactta ataaactctt ctctaaaaca ggtactattc tattcatcta
     1441 tatttccaac tttcaaaatt tctttatcaa ttctatttaa tagtttatca gagtaatccg
     1501 atttaacttt tcttacaaat aatttttcac tttcttttat ttgatctata tccctttcat
     1561 caaaccattt gttcatggaa ggatcaataa tatgaatatt tgctaatttc gaagtttgta
     1621 aagtagtttc ttcaatattt ttcaattcaa gtggtaaatc tggtcctcca ataacatttt
     1681 tatttttgaa cgaggaatta aaaaaaagtg tatgaaa
//
LOCUS       DAYYDS010000193         1674 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000193 DAYYDS010000000
VERSION     DAYYDS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.202"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..1622
                     /locus_tag="ACHA02_002923"
     CDS             75..1622
                     /locus_tag="ACHA02_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8671180.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT          561 a          306 c          343 g          464 t
ORIGIN      
        1 tgaaccccta tttcttgtgc gtttcttgtg gtattctgta acaaaatcag ttcgattcaa
       61 ggtggtgaaa aattttggaa actacagata cgttgcttca gttgcttcaa gaagcccata
      121 aaaccaatca agcacaacaa caaaccattc agaatcttac aactgaaatc caactattga
      181 atgaaaaggt caattattta accaacaaat tatttggacg ctctaaagag actctctttg
      241 aggaaacgaa tgggcaattg aatcttttta gtgatgaaga aatttctgta agtgttccag
      301 aagcagctgc caccattatt ccagtaaaag gacatcaacg agttgtagga acgaaagcag
      361 acaaaatcaa acacttacct ataacagaga aagaacatct ccttcctttg gaagaacaat
      421 tttgtgaaca ttgcggttca cagatgaaag atatcggacg gacaaaagta cgagaagaaa
      481 ttcgtttcca tcaggctatg ttggattgtc tcacccatta tcaacatacc tattgttgta
      541 aaagctgcga aaaagaagga ctttcttctt ttaaaaaagc gatcgttcct aaacctttga
      601 tttctaatag cttaggttcc aatagtttag tggcggaaac gatccggatg aaatttggtc
      661 agaaagttcc tgcctatcgg caagagaact attggaaaca aacacatggg ttggatattt
      721 cgagagataa tatcaccaat tggcatatta aagcagtgca aaacgcgtta gaccctttag
      781 gagaacgatt aagagtctat cttaatcaag aagagattct acacggggat gaaacaagtt
      841 atcgtgtgat tgaaagtgcc aaaacagata cctattactg gcagttttgt acaggaaaag
      901 acagtcagca tcccatcgtt tattatcacc acgacgaaag tcgtgctgga gatgttccga
      961 aaacttttct caaggagttt acagggtatt tacactgtga cggctatagc gggtataatg
     1021 ccgtggaaag tgtacgactt gtctattgtt tcgcgcatgt tcggcggaaa tttttcgaag
     1081 caattccaaa aggaaagaaa aacacggata ttcccgcagc tcaagcagtc aaacaattgg
     1141 ataaatggtt tgtcctagaa aagaaatgga aagacttttc tcctgagaag cgattaagct
     1201 gtcggcaaca agaactgcgt ccattattta ttgcttttta tgagtggatg gcaacgatag
     1261 atcctgttgc aaaatccaag ttggatgcag ccgttcaata tgcgtgtaaa ctacgaagtg
     1321 gttttgaacc tatttttgaa gatggtcgtt tagaactcac aaataatcgt gcggagcgaa
     1381 atatcaaaga gctggtgata ggacggaaaa attggttgca ttccacaagt cttgaaggag
     1441 cacgaacttc aggaatcatt ctgagtgtat ataaaacagc agaacttaac ggattgaatc
     1501 ctgtaaagta tctagagttt ctatttgata agataccaaa tcttcccgta ctttcagcag
     1561 aaactttaga tcaactgttg ccttggaaca aggatgtaca acaacatttt tctcgtaact
     1621 aaaaacgcca aacattcccg attctattct ataggtcggg ttatttgacg taca
//
LOCUS       DAYYDS010000194         1664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000194 DAYYDS010000000
VERSION     DAYYDS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.203"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHA02_002924"
     CDS             131..505
                     /locus_tag="ACHA02_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8671181.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHA02_002925"
     CDS             complement(619..1248)
                     /locus_tag="ACHA02_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8671182.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHA02_002926"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHA02_002926"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAYYDS010000195         1592 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000195 DAYYDS010000000
VERSION     DAYYDS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.204"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHA02_002927"
     CDS             <1..969
                     /locus_tag="ACHA02_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8671183.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="ACHA02_002928"
     tRNA            1159..1232
                     /locus_tag="ACHA02_002928"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          530 a          270 c          313 g          479 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttataa ttggagtggt ag
//
LOCUS       DAYYDS010000196         1584 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000196 DAYYDS010000000
VERSION     DAYYDS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.205"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..454
                     /locus_tag="ACHA02_002929"
     CDS             <1..454
                     /locus_tag="ACHA02_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671184.1"
                     /translation="FFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNR
                     YGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNE
                     YTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            441..>1584
                     /locus_tag="ACHA02_002930"
     CDS             441..>1584
                     /locus_tag="ACHA02_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8671185.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIP"
BASE COUNT          510 a          209 c          251 g          614 t
ORIGIN      
        1 cttttttgat caacctttat tatctgatgg ttatggcata gatgaaacag atattttcgc
       61 taaagtaaat agtatatttt ctggaaaaaa agaaacttta attaagcttc atcctagagc
      121 aaggacaaat cgttatggtg aaaaaaacat attgaattcc agtttaccgt ttgaagtctt
      181 gctacttgaa aatgacttct cttcaacact tttgattagt cctatatcta caatttcttt
      241 ttcaccatct atgatgtttg aaaagacgat caactctatt atgttggcta aattaatttt
      301 aaatgaatat acagcattat ctgaagataa aaaagaaaca ttgacaaata ttgttctttt
      361 ctgtgaacat tataaccgtc tatcagaaga aaaaatttta ctcccagaaa actgggcaga
      421 attaagaaag ttggtagagt atgggattat ttaacacact ttccaagtca aaattagtta
      481 agtccagttt ctggtatact atagggaatt tttttattaa gggaatttca ttcataacta
      541 ttcctatttt tgtaaatatt actactcaag cagaatatgg tttgattaat aatttcactt
      601 catttgtatc aattttttcg attcttgttg ggctaagcct aaattcggca attaataatg
      661 caagttttga ctataaagat gacataaatg aatttatgtc atcggtactt tctctagcta
      721 cactttcgtt aattgtgttc ttattaatag ggaacatata ttttttgttt tcaaatgctt
      781 tttttgatat gaaccaactt gtatttaatt tgatgttatt acaaagtttt gctagttttg
      841 taattagtta tatccagtct tattttacga ttaactttaa ccattttaaa tatttgattt
      901 tatcttttat atctactttt ttgaatattg caatctctgt agctttgatg cagacattat
      961 attttgatga taaatatgtt ggcagagtag tcggcggaac actgtcattt gtgttgatag
     1021 gtttagtaat ttatttaaga ataatgataa agggtgctca tttttttgat aaaaaacagt
     1081 ggagatacgc tttaaaaatc tctttgccgc taatcccaca ttccttagca aatatagttt
     1141 tagcacaatt cgatagaatt atgattaatt cttatttagg tcctgctgcg gggggaatat
     1201 attcctacat tagtaacata ggtattattc taagcgtaat atggatgtct actaataacg
     1261 cctgggttcc atggttctat ggtgagatga gtaaaaataa tgaaaaaaaa gtgaagaaag
     1321 ttagtaacta ttatttagta gtctttactt tgattacgat gattgtaatg gtagtttcga
     1381 ttgatttagc aaaatttatg gctccgaaag actattattc tgggttatct ttagtggttc
     1441 cgataacctt aggctatttc tttcagtttt tatatagttt accagttaat gctgaatttt
     1501 atgagaaaaa aacatcgttt attgcgattg gtacagtagc aagtgctata gttaatatac
     1561 tattaaacat gattttcata ccta
//
LOCUS       DAYYDS010000197         1551 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000197 DAYYDS010000000
VERSION     DAYYDS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.206"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACHA02_002931"
     CDS             132..344
                     /locus_tag="ACHA02_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671186.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ACHA02_002932"
     CDS             506..1024
                     /locus_tag="ACHA02_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFM8671187.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ACHA02_002933"
     CDS             1031..1543
                     /locus_tag="ACHA02_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671188.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          611 a          181 c          241 g          518 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt a
//
LOCUS       DAYYDS010000198         1532 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000198 DAYYDS010000000
VERSION     DAYYDS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.207"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1488
                     /locus_tag="ACHA02_002934"
     CDS             22..1488
                     /locus_tag="ACHA02_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8671189.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          422 a          289 c          325 g          496 t
ORIGIN      
        1 taaagaattg gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg
       61 aagggattgt caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg
      121 cgtgcattac ttatctatta tatgtacgat acagttgcca acgggggact tggattacca
      181 cgagcaacag cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc
      241 gtcggcggat gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga
      301 gttttcatta tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt
      361 gtttcgatgg cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg
      421 gtcggacatc tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac
      481 atgggtgtca acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa
      541 tataactatc atttagggtt ctctttagct gctattggga tgttctttgg attattacaa
      601 tacatcatcc aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg
      661 acaactgaag aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct
      721 gcagtattcg gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca
      781 atcagtgtat tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa
      841 gatgtaactg ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca
      901 atcattttct ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa
      961 cgtacacaat ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat
     1021 ccaattttca tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa
     1081 cgtcaaccat ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac
     1141 ttgctattga tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg
     1201 ttgttgggaa gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg
     1261 tctgtgacaa caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc
     1321 ttagctgatg cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga
     1381 acagaatctg cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta
     1441 tttactgtga aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata
     1501 aaaacaggaa ttgcttattt gcagcagttc ct
//
LOCUS       DAYYDS010000199         1508 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000199 DAYYDS010000000
VERSION     DAYYDS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.208"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..221
                     /locus_tag="ACHA02_002935"
     CDS             <1..221
                     /locus_tag="ACHA02_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8671190.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            218..1237
                     /locus_tag="ACHA02_002936"
     CDS             218..1237
                     /locus_tag="ACHA02_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8671191.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            1426..1499
                     /locus_tag="ACHA02_002937"
     tRNA            1426..1499
                     /locus_tag="ACHA02_002937"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1460..1462,aa:Ile,seq:cat)
BASE COUNT          492 a          284 c          315 g          417 t
ORIGIN      
        1 ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta
       61 ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca
      121 tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca
      181 aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa ttactattac
      241 tgcgatgagc ctgttaacgg ctcttttttt attgccaatt aacggatttg cctatactat
      301 caacaatgaa tttaatttgg gcgcaaatga aggtagctca caagtagcaa ataatcagta
      361 tattttactg catgaaacgg ctaatgaaac agcaacagga cgcaatgaag cgcagtatat
      421 gcaacgttca tggactagcg cttatactgc ttatattgtg ggagacggcg gaattgttta
      481 ccaagtcgga caacctggtt atgtacagta cggtgctggt tcatatgcta atgccaatag
      541 tcctgtgcag attgagttac aacacacaca tgataaagca acttttgaaa aaaactataa
      601 agcatacgtt gaattggcta gagattcagc aatgaaatat ggtattccat taacattaga
      661 cacgccttat aaccaaccag gaatcaaatc acatttatgg gtaactcaaa acatttgggg
      721 cgatcacact gatccgtatg gatacttatc agaaatgggt gtaagtaagg aaaaactggc
      781 ttacgattta gctcatggat ttacagacga aaacccaacg acttctgaga acaagcctgt
      841 cattgatcca acccgagcag gtgcagcaaa tccaacttta tcagatggaa cgaaccattc
      901 tcacattgat cagtttggag aaatcgaaaa tgcgaactta cacgtcgctg gatggcacat
      961 cgctaactat aagtacgagt atattttcat tatggactac aatactggga aagaattagc
     1021 tcgagtaaga gctgatggaa tttatagacc ggatgtaaat caagcttata atacttctgg
     1081 aaatgttggc tatcatgtat cttttaacat gcgtaacttc cctaataaga aagtctatgt
     1141 catgatgcgg gcaacgaatg atccagaggg aaacactaaa ggcggtgcgc aagatttcca
     1201 tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg ttgaggagca
     1261 ttacataact atattgacaa ctataaaaat cattcgataa aatagtgatg ttatcgcata
     1321 tcttcactat cacccataat agtcacactc caagctatgc gataacaggt ttgttgccac
     1381 acattctact ggttgattgt ttatggcttt atgtggcaac aaccagtacc cttagctcag
     1441 ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta cagggtacat
     1501 taacgtag
//
LOCUS       DAYYDS010000200         1484 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000200 DAYYDS010000000
VERSION     DAYYDS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.210"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            286..693
                     /locus_tag="ACHA02_002938"
     CDS             286..693
                     /locus_tag="ACHA02_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671192.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="ACHA02_002939"
     CDS             707..1108
                     /locus_tag="ACHA02_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671193.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..1481
                     /locus_tag="ACHA02_002940"
     CDS             1110..1481
                     /locus_tag="ACHA02_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671194.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          570 a          183 c          259 g          472 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aactcaccta atga
//
LOCUS       DAYYDS010000201         1477 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000201 DAYYDS010000000
VERSION     DAYYDS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.211"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACHA02_002941"
     CDS             191..445
                     /locus_tag="ACHA02_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8671195.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACHA02_002942"
     CDS             446..763
                     /locus_tag="ACHA02_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8671196.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          527 a          216 c          282 g          452 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattaaccc
     1381 gtggtgctag ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt
     1441 gtgctcgact taatagaaca ctgtaagcca gtaatat
//
LOCUS       DAYYDS010000202         1451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000202 DAYYDS010000000
VERSION     DAYYDS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.212"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..625)
                     /locus_tag="ACHA02_002943"
     CDS             complement(<1..625)
                     /locus_tag="ACHA02_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8671197.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ACHA02_002944"
     CDS             complement(784..1050)
                     /locus_tag="ACHA02_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8671198.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ACHA02_002945"
     CDS             complement(1040..1396)
                     /locus_tag="ACHA02_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8671199.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DAYYDS010000203         1440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000203 DAYYDS010000000
VERSION     DAYYDS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.213"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1406
                     /locus_tag="ACHA02_002946"
     CDS             105..1406
                     /locus_tag="ACHA02_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8671200.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DAYYDS010000204         1417 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000204 DAYYDS010000000
VERSION     DAYYDS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.214"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACHA02_002947"
     CDS             complement(150..1337)
                     /locus_tag="ACHA02_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8671201.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAYYDS010000205         1374 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000205 DAYYDS010000000
VERSION     DAYYDS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.217"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..1372
                     /locus_tag="ACHA02_002948"
     CDS             join(33..474,474..1372)
                     /locus_tag="ACHA02_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8671202.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAYYDS010000206         1338 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000206 DAYYDS010000000
VERSION     DAYYDS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.219"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..234
                     /locus_tag="ACHA02_002949"
     CDS             <1..234
                     /locus_tag="ACHA02_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8671203.1"
                     /translation="QTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDI
                     DSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            239..1186
                     /locus_tag="ACHA02_002950"
     CDS             239..1186
                     /locus_tag="ACHA02_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8671204.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          443 a          189 c          281 g          425 t
ORIGIN      
        1 cagacacctg aagtttttga aatgaatgcc tcaatgtatt cttttaaacc tgaatttcta
       61 aaaaaaagta attatatttt tgatgggcaa tgtgggataa taaaaatgag agattatcta
      121 attttagata ttgatagtga agaagatttt aaatggttag aatttttgtt tcctaaattt
      181 ttagaagatt cttcggagct tcaagatatc tataattatt tatatcggag gtaaaaggat
      241 gagctgttat cttatcaccg gtggggctgg cttcattggt tctaccttag ccaatcattt
      301 aggagaagaa catgtggtaa tcgtcgtcga tgacttatca atgggaaaag tagaaaatct
      361 tgatatgacg aaaaatatca cttttattga gggagatgtc gctgagaaat ccttgatgga
      421 agagatcatg aaagctaata aatttgatta tatctttcat ttagcagcag ttgctagtgt
      481 ggcagattca gttgcccgac cagtagaaac acatcgtgtg aattttgaaa gtgtattaat
      541 gattttagaa ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc
      601 agcggctgtt tatggggatg aaccgacttt accaaaaaaa gaagagtccg tgattcgccc
      661 attgacgcct tatgcggttg ataaattcgc tgcagaacaa tatgtgctag attattgtca
      721 tttatatgat gtacccggta gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa
      781 tccgaattct ccatacagtg gtgtgatttc tattctcgta gatcgctata agaaacaaat
      841 cgtaggagaa gcaacttcat ttacattatt tggtgatgga agccagtcta gagactttgt
      901 ttatatagat gatgtaatcc aagcattact tttagttgcg aatgaggaag ctgcactggg
      961 gaaacaattc aatgttggaa cgggtaaagc aaccacactt ttggaattga ttcatgcaat
     1021 caatgaaatt ctcggtgtag aattgacact tgaatacaaa gaagagcgtt cgggtgatat
     1081 ccatgactct ctagcagata ttactaagat ccagtcaatt ggctatcaac cgaaatatgg
     1141 tattttaagt ggaatgacta attatttgaa aactgaaatc aattaaaaca tactaagata
     1201 agattagagg gtaaattcag gctctttgtc aataaggact gatgagtttt gtaaaatcaa
     1261 atctgggata gaagggctct ttgtcaaatg atgttggtaa agagaaatct tagaataatt
     1321 tggacagaac agaaagaa
//
LOCUS       DAYYDS010000207         1324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000207 DAYYDS010000000
VERSION     DAYYDS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.221"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACHA02_002951"
     CDS             278..820
                     /locus_tag="ACHA02_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671205.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACHA02_002952"
     CDS             976..>1324
                     /locus_tag="ACHA02_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8671206.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAYYDS010000208         1316 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000208 DAYYDS010000000
VERSION     DAYYDS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.222"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1316
                     /locus_tag="ACHA02_002953"
     CDS             82..>1316
                     /locus_tag="ACHA02_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8671207.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGF"
BASE COUNT          446 a          257 c          251 g          362 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaa
//
LOCUS       DAYYDS010000209         1287 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000209 DAYYDS010000000
VERSION     DAYYDS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.223"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..848
                     /locus_tag="ACHA02_002954"
     CDS             <1..848
                     /locus_tag="ACHA02_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8671208.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DAYYDS010000210         1265 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000210 DAYYDS010000000
VERSION     DAYYDS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.224"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1099
                     /locus_tag="ACHA02_002955"
     CDS             20..1099
                     /locus_tag="ACHA02_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8671209.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          376 a          301 c          310 g          278 t
ORIGIN      
        1 tagacaagga tgaattcaca tgacgcaaac ccatgataac acagtttcac gcaagggaaa
       61 acatctttcc tactcagaac gttgccaaat cgctatcctg aaaaaggaag gctattccaa
      121 ccgtcaggtt gccaaagtgc tgggacgtgt tccgcaaacc ataaacaatg agattgatcg
      181 cggaaccatc acccagttaa aacgccaaac gcaaaatgga aaagtgtatg acacctacta
      241 ttctgtttat gacgctgatg caggacaggc cttctatgaa aagcaacgac tgaactgtgg
      301 cagacgcccc aaatgggcga acacaaatgc cttcattgat tgggcagatg acaaaatgtt
      361 aaatgaaaaa tggtcgccgg acgtcgtggt cggatttgcc agaaaacacg agctgttcga
      421 ttgtgccatc attcctggca caaccgctct ttatcaatgg attgaccgcg gaatcatgcg
      481 aacaaagaac atggacttgc ttgaaaaact gtcccgtaaa atcaaagcgg tccgcccagg
      541 acaccgtccc aacaagcgtg ttctcgggac gtcaattgaa gaacgcccta aagaagttga
      601 aacacgggaa acgttcggtc attgggaaat cgacacggta gtgggaaaca agaccaagtc
      661 ggatgccgtg ctgctgacgc tggctgaacg gcagacacgc tttgaagtca tcctaaagtt
      721 gaacgggaag gacaccgaat cggtggacca agcggttcaa cagctaagag aacgggctgg
      781 cgctaacttc gatcaattat ttaagaccgt tacatccgac aatggctcgg agttttctgg
      841 gctacacgat gctctgcaag atgtagctga cgtctatttc agccacccct atgcttcttg
      901 ggagcgtggg acgagcgaaa accagcacaa attaattcgc cgtttcgtcc ccaagggaag
      961 cccgattagt gaggtgagcg agaaccagat tttgcggatc cagcgttgga tgaacgatta
     1021 cccgcggaaa attttgggtt atgcgacacc acatgataca ttcgtccagg cattcaagca
     1081 agaacggttg gtggcttgac ccattattcc ttgataacga tcctcactcg ggacatggct
     1141 tcatgtctgg aaagctggcc tggggccaga atcgtctgaa gactatcata cctcagttca
     1201 ttatcaaggc ctcactgatt tagaataacg aggcagttac ggtatcaaac aaatgagtta
     1261 cctgt
//
LOCUS       DAYYDS010000211         1238 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000211 DAYYDS010000000
VERSION     DAYYDS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.225"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..597
                     /locus_tag="ACHA02_002956"
     CDS             <1..597
                     /locus_tag="ACHA02_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8671210.1"
                     /translation="MDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDR
                     IHWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKK
                     YLIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKS
                     MLNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            789..1187
                     /locus_tag="ACHA02_002957"
     CDS             789..1187
                     /locus_tag="ACHA02_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFM8671211.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          453 a          151 c          236 g          398 t
ORIGIN      
        1 atggatgttg atcaaaatct tgaaacggaa ctaaaaacca tagttaacac tttgcaaaga
       61 caagcagttt atgaaatttc gaatctagac aaaaaatata agacgtatac tactgtatct
      121 gctattgatc ggattcattg ggatttagaa gagcatagtg tatctttcag tttttcagaa
      181 gatgtaattc ccttaattgt ctcaatcaaa aagaagtatc cgttctttgt ttttgaagac
      241 ttagcatctt ttcaaaaaaa atatagcatc gtgttataca aatatttggc gatgtattat
      301 aaaaaatatc ttatcgccaa aaataaagga gattttgaga aagctgaaat atataagaat
      361 cccctgattg aaatagttaa aattcgtgag attacgaaaa cagaaaatga gtataaaaca
      421 atatcgaatt ttgatagacg tgttttaagg gaatcagttg aggaaattaa tgaaaaatca
      481 atgctgaata ttgattttgc taaagttaga aaaggcagaa attacaattt tgctgtccag
      541 tttaatattt ctagtgagtt tgatgaaaaa acttatagag aaaaaaaatt aggttaatgc
      601 aaaatatatc ttactcgttt gaagatgctt cagacgtatg ctaagatata tagtgccttg
      661 ggagcaaggt attaatctcc tttagaggtt aattagcttg tgatcgccta tcaatttttt
      721 tggtaggcga ttcttttttt tgaaacaaaa aagcaaaaat tattttttaa ttagagaggt
      781 gcaatgcgat gaaatacttt ttttgtgcag tattcgaatg gaaaggaaat gaatatgcag
      841 ttaaattccc agattttcca gacttatata cacatggtga aactttagaa gatgcttttg
      901 agatggcgca agaagtattg gaaggatact tggtgctttt agagttgcaa gaaaaagaaa
      961 ttccatcgcc tacgcctgtt caagagttaa atgcaagaat caagcgatct gagaatgtta
     1021 atttatttgt cgtaaaagct aatacgaaag ttgctaaagc tcgaaacaag caaaattatg
     1081 atcgaaaatc agtgactgtg gaacactatt taaatgtttt agcggctgaa gagaatatta
     1141 atctgtcgaa agctttgaat gatttgttga gagaaaagtt gatttgatgt ctctcagatg
     1201 atcatattaa gcaaaaacag cttgttttag gacgtatg
//
LOCUS       DAYYDS010000212         1213 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000212 DAYYDS010000000
VERSION     DAYYDS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.226"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1213
                     /gene="aph(2'')-I"
                     /locus_tag="ACHA02_002958"
     CDS             <1..>1213
                     /gene="aph(2'')-I"
                     /locus_tag="ACHA02_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFM8671212.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYY"
BASE COUNT          508 a           95 c          209 g          401 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tat
//
LOCUS       DAYYDS010000213         1207 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000213 DAYYDS010000000
VERSION     DAYYDS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.227"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1207
                     /locus_tag="ACHA02_002959"
     CDS             <1..>1207
                     /locus_tag="ACHA02_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFM8671213.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMR
                     IRIFLINQL"
BASE COUNT          400 a          234 c          232 g          341 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt ttgatcaatc
     1201 aattaat
//
LOCUS       DAYYDS010000214         1195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000214 DAYYDS010000000
VERSION     DAYYDS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.228"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHA02_002960"
     CDS             <1..1076
                     /locus_tag="ACHA02_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8671214.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAYYDS010000215         1149 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000215 DAYYDS010000000
VERSION     DAYYDS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.230"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1131
                     /locus_tag="ACHA02_002961"
     CDS             4..1131
                     /locus_tag="ACHA02_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8671215.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          414 a          199 c          221 g          315 t
ORIGIN      
        1 taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat caatgatgat
       61 ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc cgaagctgct
      121 ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg tacggatact
      181 cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa tctaaaatac
      241 aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta tttaggaaag
      301 aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc tgaatttaaa
      361 aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa tgcgctgaat
      421 atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt aaaaatacca
      481 aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct tgaggacttt
      541 gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt gaatattgga
      601 ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga tgtcgatttt
      661 tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc atgggtatat
      721 ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac cttgctagca
      781 attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg aaagctctac
      841 attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc aagtgtagtg
      901 aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt ccattctctc
      961 agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga aatctctgaa
     1021 cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt aacagataag
     1081 atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg atttttgcca
     1141 ccgaaaaaa
//
LOCUS       DAYYDS010000216         1141 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000216 DAYYDS010000000
VERSION     DAYYDS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.231"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACHA02_002962"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACHA02_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8671216.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          337 a          232 c          194 g          378 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 t
//
LOCUS       DAYYDS010000217         1114 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000217 DAYYDS010000000
VERSION     DAYYDS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.232"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..897)
                     /gene="vanY"
                     /locus_tag="ACHA02_002963"
     CDS             complement(<1..897)
                     /gene="vanY"
                     /locus_tag="ACHA02_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFM8671217.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          399 t
ORIGIN      
        1 agtatgtgtt gatccgggaa acactgtcac aattacaccg tctatattgt ttcctgatat
       61 ctcataacga agattagtcg gcacatgaat ggtggtattt ttagtaacag gataataaaa
      121 gatctcatat ttttccccat ttacactaac agaaatggtt ttttcttctt ttaggtaatc
      181 catatattcc tcgagaacga aattcttttc tttcataatc gcactatgtg gtaaaccaac
      241 atagcgaata tgccatggtt catattgaat tcctgttaac tctgttttgt cctctggata
      301 acgtaaaatg aacccgtatt tccaagcatt ttcttctatc cactttcctt caggggctcg
      361 ttccattttc gtcaagcttg atcctacatc tagtgataaa cctgaattat gctcactata
      421 acctgctggt aaggcatact cagcccccat ttcttggtaa agcacacttt gctcatcaaa
      481 gtctcgatag ccactattaa taataaaatg actaacgcca ccctttacag catcattgac
      541 catctctgaa aatttttgtg ctatttcttt tgacatataa atattactat caagcaaccc
      601 gtatccattt attaattcgt catgtttaga taaattcacg atatctgact tcacactttc
      661 ttggcgaaca ggatatttac tattgattaa tagcagattt ccttgataaa cctgttcttc
      721 tgtaattgtt ttctcttggg tattttcaga agtcccacta ttttctaaat gttctttggg
      781 attttgatca taattttgaa attcgacttt ttcctgagaa aacagtgctt cattaacgta
      841 gtcataacct aagtatatta agaataacaa taacaataaa aaaaacaact tcttcatttt
      901 cagtctcctc cttaactata tagttggaag tatgaaatat ttctagaatc ttccccttaa
      961 acggcacaat ctcgtttgag attgtgccgt tttgccccgt aaattacagt caattatttc
     1021 gccctactgg atttactatt atcaccaatg tagatgaatt ttcataagtg atacccatgc
     1081 tgaatttaac gaggctgaac tgcctgttga caat
//
LOCUS       DAYYDS010000218         1063 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000218 DAYYDS010000000
VERSION     DAYYDS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.233"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..153
                     /locus_tag="ACHA02_002964"
     CDS             <1..153
                     /locus_tag="ACHA02_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8671218.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            207..575
                     /locus_tag="ACHA02_002965"
                     /pseudo
     CDS             207..575
                     /locus_tag="ACHA02_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            681..944
                     /locus_tag="ACHA02_002966"
     CDS             681..944
                     /locus_tag="ACHA02_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8671219.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1063
                     /locus_tag="ACHA02_002967"
     CDS             944..>1063
                     /locus_tag="ACHA02_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8671220.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPY"
BASE COUNT          407 a          132 c          215 g          309 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tat
//
LOCUS       DAYYDS010000219         1059 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000219 DAYYDS010000000
VERSION     DAYYDS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.234"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..993)
                     /locus_tag="ACHA02_002968"
     CDS             complement(37..993)
                     /locus_tag="ACHA02_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671221.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgt
//
LOCUS       DAYYDS010000220         1051 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000220 DAYYDS010000000
VERSION     DAYYDS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.235"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACHA02_002969"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACHA02_002969"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8671222.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAYYDS010000221         1045 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000221 DAYYDS010000000
VERSION     DAYYDS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.236"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            412..1045
                     /locus_tag="ACHA02_002970"
                     /pseudo
     CDS             412..1045
                     /locus_tag="ACHA02_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          360 a          183 c          186 g          316 t
ORIGIN      
        1 attttattat taataaaata ttttttatat tacaaaagta gtgtttcaaa gttagtagag
       61 aaaatgtagt tatatattta aatttttacc agaaagaaat gaacaccttc aattatctaa
      121 atctcgaaaa tggttagatg taatcatcag gaaaaacaat cttaaacgaa ttactattca
      181 tggtcttaga cacacacatg ctagcttgct tttagcaagc tagtgcaaat attaaagatg
      241 tgcaagagcg tttaggccac tcttctattc aaatcattat ggatctatat attcatatta
      301 cagacaaaag aaaagaagaa accgtagcgc aattcgcaaa atataggctc tataatttat
      361 gggactgatg aatcaaaatt gattcttcag tcccattgtt attttaggta tatgattcgt
      421 acattgaaag agttgaaaaa ctatcttccg aagccgtcaa aaaggaccct gccaaaagta
      481 ttgatggtgg atgaatttcg ttcgcacgct tcaattgtgg ataaaatgag ctttatttgt
      541 gcggatggcg agactggaaa actcattgat attttgccta cacgaaaatt gtctcgatta
      601 acacactatt ttagtcgctg tgcaaatcct gaagcggtgg aatatttagt gacagatatg
      661 aacgccgcct acttccaact gacaaaacgt gtcttaccaa atgcgaaagt cgtcattgat
      721 tgttttcata tcatcaaaca tttgaatcag gcgttcaatg attttcgtgt acaagaaatg
      781 aaaagattac accagttagg gcaacgacgg gaagccgaga aagtgaaaaa gaactggcgc
      841 tttcttctaa aaaaccgtca aaaaatcaat cactatgatt acaaaacatg gcgcagtttt
      901 cgtgcaccta aatacccgct actcactgaa gcaatgatga ttgatcgatt gctggcattt
      961 tcaacgcctt taaaggacgc ctatactttt ttccatgagc tgaccgaagc ctttagagac
     1021 aaggaatcag acttattttt tacct
//
LOCUS       DAYYDS010000222          997 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000222 DAYYDS010000000
VERSION     DAYYDS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.238"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..913)
                     /locus_tag="ACHA02_002971"
     CDS             complement(26..913)
                     /locus_tag="ACHA02_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8671223.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DAYYDS010000223          994 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000223 DAYYDS010000000
VERSION     DAYYDS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.239"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="ACHA02_002972"
     rRNA            complement(<1..>994)
                     /locus_tag="ACHA02_002972"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAYYDS010000224          988 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000224 DAYYDS010000000
VERSION     DAYYDS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.240"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACHA02_002973"
     CDS             <1..704
                     /locus_tag="ACHA02_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8671224.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          335 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataa
//
LOCUS       DAYYDS010000225          969 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000225 DAYYDS010000000
VERSION     DAYYDS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.241"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..>969)
                     /locus_tag="ACHA02_002974"
     rRNA            complement(1..>969)
                     /locus_tag="ACHA02_002974"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          214 a          302 c          210 g          243 t
ORIGIN      
        1 taagtcctcg atcgattagt atcagtccgc tccatacatc actgtacttc cactcctgac
       61 ctatctacct gatcatctct cagggatctt actttcttaa agaaatggga aatctcatct
      121 tgaggtgggc ttcacactta gatgctttca gcgtttatcc cttccctaca tagctaccca
      181 gcaatgccct tggcagaaca actggtacac cagcggtaag tccatcccgg tcctctcgta
      241 ctaaggacag ctcctctcaa atttccaacg cccgcgacgg atagggaccg aactgtctca
      301 cgacgttctg aacccagctc gcgtgccgct ttaatgggcg aacagcccaa cccttgggac
      361 cgactacagc cccaggatgc gacgagccga catcgaggtg ccaaacctcc ccgtcgatgt
      421 ggactcttgg gggagataag cctgttatcc ccagggtagc ttttatccgt tgagcgatgg
      481 cccttccatg cggaaccacc ggatcactaa gcccgacttt cgtccctgct cgacttgttg
      541 gtctcgcagt caagctccct tctgccttta cactcttcga atgatttcca accattctga
      601 gggaaccttt gggcgcctcc gttacctttt aggaggcgac cgccccagtc aaactgccca
      661 tctgacactg tctcccacca cgattagtgg tgcgggttag agtggccata acgcaggggt
      721 agtatcccac cagcgcctcc atcgaaacta gcgttccgat ttctacggct cctacctatc
      781 ctgtacatgc ggtacagaca ctcaatatca aactacagta aagctccatg gggtctttcc
      841 gtcctgtcgc gggtaacctg catcttcaca ggtactaaaa tttcaccgag tctctcgttg
      901 agacagtgcc caaatcgtta cgcctttcgt gcgggtcgga acttacccga caaggaattt
      961 cgctacctt
//
LOCUS       DAYYDS010000226          915 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000226 DAYYDS010000000
VERSION     DAYYDS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.243"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>915)
                     /locus_tag="ACHA02_002975"
     CDS             complement(109..>915)
                     /locus_tag="ACHA02_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFM8671225.1"
                     /translation="TYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGL
                     QLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDL
                     TYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDR
                     GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLR
                     LELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          245 a          212 c          177 g          281 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc gccgactgag
      781 ttgaagccca cgctttgcta aacacttctt caattttcgg gtaccgtaag cctttcggtt
      841 tcgaataaac tcttcctgaa cgatctcttc taagtcggac tcattttcga tcggtttcgc
      901 ttgataatag taggt
//
LOCUS       DAYYDS010000227          907 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000227 DAYYDS010000000
VERSION     DAYYDS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.244"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..>907)
                     /gene="menA"
                     /locus_tag="ACHA02_002976"
     CDS             complement(132..>907)
                     /gene="menA"
                     /locus_tag="ACHA02_002976"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8671226.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT          324 a          185 c          159 g          239 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaat
//
LOCUS       DAYYDS010000228          904 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000228 DAYYDS010000000
VERSION     DAYYDS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.245"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..888
                     /locus_tag="ACHA02_002977"
     CDS             265..888
                     /locus_tag="ACHA02_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8671227.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaa
//
LOCUS       DAYYDS010000229          899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000229 DAYYDS010000000
VERSION     DAYYDS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.246"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>899
                     /locus_tag="ACHA02_002978"
     CDS             <1..>899
                     /locus_tag="ACHA02_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8671228.1"
                     /translation="KGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKI
                     NEVQYTQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQ
                     DTRELQYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKT
                     LHGCYRSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIK
                     EKIESGIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLA
                     ILYFTESGEKVRRRKLIANDILI"
BASE COUNT          341 a          120 c          148 g          290 t
ORIGIN      
        1 caaaaggatt tacaattatt gatgattata ctcaaagagc ttacgtcagc caaattaata
       61 atatgctgaa tttaaatctc aaaacgtatg aactgaacaa aattgatttg tcactttctc
      121 atgattttaa aattaatgaa gttcagtata ctcaagtttc tgaaatgtat cacactatgt
      181 tggaaaaaaa taaagaaatt gattttgata tgattttact agtagcgtat aaaattttaa
      241 ctgtaaacag taacgttgca cgtaatattg ctagtaatat aagggcgatt tatgtggatg
      301 aatttcaaga tactagagaa ttacaataca atgtaatagc taaattactt caaaacaacc
      361 ctcaaattca gtcaatgttt gttggagata ttgaccaagc aatttatgga tcattagatg
      421 gtatagctaa atcagttgaa gaactagaaa tgctaactag tcacactttt caatcaaaga
      481 cgctacatgg ctgttacagg tctaatcaga gattagtaga cttctactct aattttcaga
      541 gttgtcctta cgagattgaa agcagaggga ataataaaaa cgaccgtggt tttataagtt
      601 atgattgcaa agttactaag gaggatttac ccaacataat aaaaaatata ataaaggaaa
      661 agattgaaag cggcattcat gaaaaagata tttgtgtact tgctcctcaa cactatcctc
      721 tgttttcact gggtgagtat ttaaagaaag aattaccgta ttgcaatttt gatgctataa
      781 atatatcacc aattaaggta aataatcata gtttattttt caagttagca attttgtact
      841 ttactgaatc aggtgaaaag gttcgaagaa gaaagctaat tgcaaacgat attttaatt
//
LOCUS       DAYYDS010000230          882 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000230 DAYYDS010000000
VERSION     DAYYDS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.248"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(201..599)
                     /locus_tag="ACHA02_002979"
     CDS             complement(201..599)
                     /locus_tag="ACHA02_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8671229.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(596..832)
                     /locus_tag="ACHA02_002980"
     CDS             complement(596..832)
                     /locus_tag="ACHA02_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8671230.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          277 a          159 c          122 g          324 t
ORIGIN      
        1 taaaaaatca gcccactagt tctaaaccac tatttcctac tattcactct tttgtattcc
       61 ttttaaatca attatttcaa aattaatcca tattttccat atttttatga aaaaactatt
      121 ttgtatgatt aaacataaaa aaatcagcat attccttaat gacatgctga tctaattgag
      181 aaataaagtt tttaattcgt tcattttatt gcggtttgtc gtaaataact ggttatcttg
      241 gactttagtg aatcgaaatc caatgaacta gtggtaatca ttatgataaa atcaacagct
      301 tcttgacgag aaaaagcaac tggacaatta ttcaattgaa gaaacaacaa catggcaaca
      361 agagcagttc gtttattgcc attatgaaaa ggatgttttt tagctaaatt aattgctaaa
      421 atcgccgctt tttcatagat ttcaggatag agttcttgat tgaatactgc ttgtgaaggc
      481 tgtttgacgg ccatatctaa tgcatttgca tcttttacac cgatcatttc gcctggcgag
      541 tgacgtaaaa taactttcgc gttgattttg ataatttctt tagtcgaaag atacctcata
      601 aatcaaccaa atcttttaac gcgtcttcat attgattcat gatcgaatca actaatgaat
      661 caatatcagg ttcttcttgt gctttgatca ttcgtacaga accatcagat tgaaaaacat
      721 aactgattgc gtcacctgtc tgcacaccca tgaattcttt tacttctggt gggatagttg
      781 ttacatcgct attgccagat tttctaattt tatacttttt catttttttc atatttcgcc
      841 accctctata ccattctcat tctatagtaa gtacattata tc
//
LOCUS       DAYYDS010000231          838 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000231 DAYYDS010000000
VERSION     DAYYDS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.249"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..>838
                     /locus_tag="ACHA02_002981"
     CDS             19..>838
                     /locus_tag="ACHA02_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFM8671231.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          289 a          123 c          155 g          271 t
ORIGIN      
        1 agaaaagagg acttccatat gaacgatttt actacagaaa ttatggaaat actaatcaat
       61 aaaggtgatt tggatgattt gtttcgtcgt cacctagaat tggctattaa cgctttatta
      121 caagctgaat taacggtatt tcttgactat gaaaagtatg atagagctgg atttaattca
      181 ggtaattccc gtaatgggaa ttattcgcgc tcttttaaaa cagagtacgg tgaattgaat
      241 ttagtaatac ctagagatag gaatggagac ttttctcaac aaactttacc tgcttataaa
      301 agaaccaatg actctttaga aaccactatt attcagctag ttcaaaaagg aattactatg
      361 tccgaaatct ctgatctaat cgaaaaaatg tatggtcatt attacaaacc acaaactatt
      421 tcaaatatga gtaaaattgt gtctgaagat gtttcggcat ttaaagaaag aactttagaa
      481 gctaaatact cagtcatttt tatggatgct actcatattc ccttaaaaag acaaaccgta
      541 tcaaaagaag ctgtctatat agtaattggt atcaggttgg atggaactaa agaggttcta
      601 ggatttacta tagctccaac tgaatctgct tatgtttgga aagagattct tcaagatcta
      661 aaagaccgtg gtttagaaga ggttttgtta gtcgtaacag atggtttaag tggtattgac
      721 gatagtattc atagtgttta tccaaatgct caatttcaac aatgttgtgt ccatatatct
      781 agaaatatcg ctcataaggt tcgtgttagt gatcgacaag aaatctgtag cgatttca
//
LOCUS       DAYYDS010000232          811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000232 DAYYDS010000000
VERSION     DAYYDS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.250"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACHA02_002982"
     CDS             <1..672
                     /locus_tag="ACHA02_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8671232.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAYYDS010000233          789 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000233 DAYYDS010000000
VERSION     DAYYDS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.251"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            327..725
                     /gene="spxA"
                     /locus_tag="ACHA02_002983"
     CDS             327..725
                     /gene="spxA"
                     /locus_tag="ACHA02_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8671233.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          288 a          109 c          144 g          248 t
ORIGIN      
        1 tattaaagaa ataaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga
       61 taaaataata gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt
      121 gaaaactcta atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac
      181 cttagataac tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg
      241 attatgtata atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt
      301 cttatcacga aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt
      361 cctgcagaaa ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata
      421 ttttttcaga accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg
      481 gaacagaaga aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag
      541 atgatctgcc gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc
      601 gtccaatcat gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc
      661 ggttcctacc gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat
      721 tatagaagga taatacgaaa cttggaaaag actggctctt tgtcaaatga tgttggtaaa
      781 gagaaatct
//
LOCUS       DAYYDS010000234          750 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000234 DAYYDS010000000
VERSION     DAYYDS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.253"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..500)
                     /locus_tag="ACHA02_002984"
     CDS             complement(75..500)
                     /locus_tag="ACHA02_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8671234.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMEGLEEAIQTTNDCPYRRTF
                     HSDQGWSYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          216 a          138 c          152 g          244 t
ORIGIN      
        1 tagtgacccc caaaagttag acttttttgg agtggagaaa tccactcttt ttttcatgct
       61 ctaaaatgat atttttacac aaccgtaacg gttgttttac gaaattggac gggactttgc
      121 cagtttaatt tctgttttat ccgttcatta ttgtagtaat agatatagct atcgatggct
      181 gattttaatt cctcgaaaca gttataaact ttcccgtgaa aaatctcttg ttttaataag
      241 ccaaaaaaat tctccataag tgaattatct aagcagttcc ctttccgaga catgctttga
      301 aagattttgt actccttcaa tttgtttcta taagctttca tttgatacga ccaaccttga
      361 tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc ttcctccaaa
      421 ccttccatta ccgcacgtgt ttctgaatgg tggtagcgac cgatttgaac ggctagggcg
      481 gaaatacggt gcaaatgcac ggtggtttca tgcaatgctt acagggcgta ttttgtaagg
      541 gtatgtaacg gagataatcc cgacggtaat ggatgtgaga ggccaagccg acgctgacgg
      601 tagggcaaac aaacatagtt gcactctagc gatcgttatt tatttttaac ggtcaaacta
      661 gggtacaact atgcttaaaa cacttcgcct caacttctgg tagcaaaagt gattttgaaa
      721 gtcaaaatat tttttatgag ttatttataa
//
LOCUS       DAYYDS010000235          733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000235 DAYYDS010000000
VERSION     DAYYDS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.254"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>733
                     /locus_tag="ACHA02_002985"
     rRNA            <1..>733
                     /locus_tag="ACHA02_002985"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAYYDS010000236          712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000236 DAYYDS010000000
VERSION     DAYYDS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.255"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACHA02_002986"
     CDS             complement(<1..446)
                     /locus_tag="ACHA02_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8671235.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DAYYDS010000237          704 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000237 DAYYDS010000000
VERSION     DAYYDS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.256"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          298 a          106 c          122 g          178 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatgctagc aaacaaattg agcttaacaa tcgcaagatt gttcgaactt
      661 tttatgagag tttgatcctg gctcaggacg aacgctggcg gcgt
//
LOCUS       DAYYDS010000238          667 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000238 DAYYDS010000000
VERSION     DAYYDS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.257"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            466..>667
                     /locus_tag="ACHA02_002987"
     CDS             466..>667
                     /locus_tag="ACHA02_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671236.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLN"
BASE COUNT          268 a           73 c          120 g          206 t
ORIGIN      
        1 attttgtgtt taattgttca tgaagtgaat aaacaattgt gaattaaaaa aataaaaacc
       61 tagtataatc actttaataa attgttggta gaaggtgatc aaaatgggag taaacgaaat
      121 gatgaaagtc agattgagtt tttaaatgat accttcctca gaacttcaaa acgaattcaa
      181 agattcgttg acaaatcttg agcaagatca tttaaaaaaa tattttctct tcatttgacg
      241 aaaaatgttt agaaatctta tataagtgaa gataaagtaa ttggtgtaat taattgtaga
      301 gaatagcctt tgagcaattg aagcgataca attatttaga aaatgaactt cctccagcat
      361 actacatgat tccttacggg acgatgttaa agaagaaagc aagaaatttt agaagtatta
      421 gtagataaac taatgggctt ttctcaattt aagggagtag cccaaatgtt tatcaaatat
      481 ggtgcagata cagtagcaac catgtttaca gaagaatatc aagtagacta ttatgaagta
      541 atagataaat gtgtacattt tgtttatgaa aaagtgtgga attttatgaa ttttgcgata
      601 agtgaagaaa tcttacgagt acaagaaaac agaacaggaa tatttaccag agacagaaag
      661 cttaatt
//
LOCUS       DAYYDS010000239          635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000239 DAYYDS010000000
VERSION     DAYYDS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.259"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..270)
                     /locus_tag="ACHA02_002988"
     CDS             complement(<1..270)
                     /locus_tag="ACHA02_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671237.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="ACHA02_002989"
     CDS             complement(276..>635)
                     /locus_tag="ACHA02_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8671238.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAYYDS010000240          605 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000240 DAYYDS010000000
VERSION     DAYYDS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.260"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..543
                     /locus_tag="ACHA02_002990"
     CDS             <1..543
                     /locus_tag="ACHA02_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8671239.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
BASE COUNT          236 a          108 c          104 g          157 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
      601 caata
//
LOCUS       DAYYDS010000241          588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000241 DAYYDS010000000
VERSION     DAYYDS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.261"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACHA02_002991"
     tRNA            complement(65..150)
                     /locus_tag="ACHA02_002991"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACHA02_002992"
     tRNA            complement(167..238)
                     /locus_tag="ACHA02_002992"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACHA02_002993"
     tRNA            complement(250..322)
                     /locus_tag="ACHA02_002993"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACHA02_002994"
     tRNA            complement(340..421)
                     /locus_tag="ACHA02_002994"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACHA02_002995"
     tRNA            complement(429..501)
                     /locus_tag="ACHA02_002995"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACHA02_002996"
     tRNA            complement(505..577)
                     /locus_tag="ACHA02_002996"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAYYDS010000242          530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000242 DAYYDS010000000
VERSION     DAYYDS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.262"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..528
                     /locus_tag="ACHA02_002997"
     CDS             40..528
                     /locus_tag="ACHA02_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8671240.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
BASE COUNT          221 a           42 c          102 g          165 t
ORIGIN      
        1 gcaatagaat tgtactagta ggataaggat ggatggaaaa tgaaaaaaac tatttttgtt
       61 ttagcgattt ttatatgttt tgggctactt ttggtaggct acttgttgtt taattctaat
      121 gaaatgagaa ctatttctac gaatgacttg ttaaaaaaaa gtgaattaaa ggataaaaaa
      181 aaagaatctc ttgcctttat aatgtttact caagaagagt gtgagtattg tatagcaata
      241 aatccaatca tagatagtgt aaataaggaa actttgaata agataaagaa atatgatata
      301 tctgtagatt ttcaatctaa acaaaagtgt aaaattaagt ttgttccgac tattgttgca
      361 tatagtaaga ataaagaagt ggggagaatt gacaatgaat tagataattt gtatcgaaaa
      421 aagcaagatg gttcaattgt aatagacaag aaaaaaacaa agacattgct tatagattgg
      481 atgaaaaaaa tggataaaca aggagaatat ttgtatggag aagattaaac
//
LOCUS       DAYYDS010000243          526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000243 DAYYDS010000000
VERSION     DAYYDS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.263"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACHA02_002998"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACHA02_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8671241.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAYYDS010000244          515 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000244 DAYYDS010000000
VERSION     DAYYDS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.264"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..>515)
                     /locus_tag="ACHA02_002999"
     CDS             complement(107..>515)
                     /locus_tag="ACHA02_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM8671242.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcca
//
LOCUS       DAYYDS010000245          503 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000245 DAYYDS010000000
VERSION     DAYYDS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.266"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACHA02_003000"
     CDS             <1..375
                     /locus_tag="ACHA02_003000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8671243.1"
                     /translation="DDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPD
                     GKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEK
                     LGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          201 a           35 c           86 g          181 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      301 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      361 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      421 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat
      481 ggtagagata aaaaatatac att
//
LOCUS       DAYYDS010000246          489 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000246 DAYYDS010000000
VERSION     DAYYDS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.268"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..398
                     /locus_tag="ACHA02_003001"
     CDS             141..398
                     /locus_tag="ACHA02_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671244.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          208 a           63 c           69 g          149 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattt
//
LOCUS       DAYYDS010000247          487 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000247 DAYYDS010000000
VERSION     DAYYDS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.269"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..377)
                     /locus_tag="ACHA02_003002"
     CDS             complement(<1..377)
                     /locus_tag="ACHA02_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFM8671245.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREY"
BASE COUNT          128 a          108 c           88 g          163 t
ORIGIN      
        1 aagtattccc tgtttagttt ctccgaaaat gattcaggca tatctggccg agattttttg
       61 tttatctttc tttttagaga acgccaaact actcggtaga tggcgagata acgcggcact
      121 ttaagccaaa tgatcttatc tgcttcttta atcctttcct ctaatgtaga agtataattt
      181 ccttcaacga tccatgacgg attagatacc ataaacaatt gctgttcttg caaaaaccac
      241 ttttttgcct gaatactgta gtctgttgta tgccataaaa ggtctagcgg cagtagcgga
      301 aagtcggtga tttctttgat cttccttgcg agggtcgatt ttcccgaccc actttgtcca
      361 atcacgatga tcttcattct tctccctcac ttctctttgg aatttggttc gatcatacca
      421 cttataacgc cgcaaaaaaa caagggactg tgacaaaagt cgtgtcaaag tcccttgttt
      481 cgaataa
//
LOCUS       DAYYDS010000248          485 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000248 DAYYDS010000000
VERSION     DAYYDS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.270"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..259)
                     /locus_tag="ACHA02_003003"
     CDS             complement(62..259)
                     /locus_tag="ACHA02_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8671246.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DAYYDS010000249          482 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000249 DAYYDS010000000
VERSION     DAYYDS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.271"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          186 a           79 c           77 g          140 t
ORIGIN      
        1 tacactcctt tgttttcttt cgtcggaaat acaatttgag tgtaccataa atgccttttt
       61 atttttctaa cttaatttta caattcgcga tatattaaac aacgttgtcg cgcttcattg
      121 aaacatttta tgaaaattcc aaaaaagagt tgacacatgt ttttagacgt gataagataa
      181 ctaagtcgtt aaaaagaacc acttgcgaag cttgattcta tcgaaaaaaa acttcaaaaa
      241 agttcttgac aaataacaaa cgataagtta tgataaatga gttgctgaaa gatttcgaaa
      301 aacgaaatcg aaaaaaactt ttaaaaaaga gttgacaatc gcttgtctct ctgatatgat
      361 ataaaagttg ctacggcagc caaaaacgaa tcgggaaaca ctcccgatga agtagacctt
      421 tgaaaactga acaaagtaag acaaaccaaa tgtgtagggc gtcttgattc aattcaagac
      481 aa
//
LOCUS       DAYYDS010000250          479 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000250 DAYYDS010000000
VERSION     DAYYDS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.272"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..293
                     /locus_tag="ACHA02_003004"
     CDS             99..293
                     /locus_tag="ACHA02_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671247.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          203 a           61 c           82 g          133 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaat
//
LOCUS       DAYYDS010000251          452 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000251 DAYYDS010000000
VERSION     DAYYDS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.273"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>452
                     /locus_tag="ACHA02_003005"
     CDS             <1..>452
                     /locus_tag="ACHA02_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671248.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DAYYDS010000252          449 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000252 DAYYDS010000000
VERSION     DAYYDS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.274"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>449
                     /locus_tag="ACHA02_003006"
     CDS             <1..>449
                     /locus_tag="ACHA02_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8671249.1"
                     /translation="ESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITA
                     DDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKE
                     KVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          182 a           67 c          105 g           95 t
ORIGIN      
        1 gaaagtggaa caacagtctt tgcagtgaaa gacggcgtaa gcttaccaga agataaactt
       61 ccagtgttga aagcaaaaga tggttataca gatgcgaaat ggccagagga agcaactcaa
      121 ccaattacag cagatgatac agaatttgta tcaagtgcaa caaaactgga tgataaatct
      181 gacgctgaca aatataatcc tgaaggtcaa aaagtgacta cagaattgaa taaggaacca
      241 gacgcatttg agggaattaa gaacaaagaa gatttaccaa aagatactaa gtatacttgg
      301 aaagaaaaag tagatgttag cgcagctgga aataaaaaag ggactgttgt agtgacatat
      361 ccagatggat catctgatga agttgaagta gatgttacag tgacagacaa tcgttctgac
      421 gccgataaat atgagccaac agtagaagg
//
LOCUS       DAYYDS010000253          443 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000253 DAYYDS010000000
VERSION     DAYYDS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.275"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..162
                     /locus_tag="ACHA02_003007"
     CDS             <1..162
                     /locus_tag="ACHA02_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8671250.1"
                     /translation="ISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHAN
                     EVLKIAEEK"
     gene            163..>443
                     /locus_tag="ACHA02_003008"
     CDS             163..>443
                     /locus_tag="ACHA02_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8671251.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDS"
BASE COUNT          170 a           52 c           92 g          129 t
ORIGIN      
        1 ataagttttg aagaaaacat tgttaaagaa atgcctgaat ctgctaatga tatgtatgag
       61 aaggagttga aatatttctt tgaaaacgta gtaacaggca aagaaaactg gaatgattta
      121 tatcatgcaa acgaagtatt gaaaatagct gaggagaaat aaatggaaat tttatttact
      181 atttgtggta gagctgggtc taaggggttt aaaaataaaa atttgaaata tttggcagga
      241 aagcccctag tccatttcgc tttagcaatg atagatatct atcaacaaaa acatcctgaa
      301 cataattgca cgacttgttt gaatacggac agtaatgagt tagtagaatt gacgactaag
      361 ttaaatccgt ctgtgaaaat tgtagaacgt aaacctgaat tagctacaga tactgtaggg
      421 aaaattgaag tgatacgtga ttc
//
LOCUS       DAYYDS010000254          433 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000254 DAYYDS010000000
VERSION     DAYYDS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.277"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            124..327
                     /locus_tag="ACHA02_003009"
     CDS             124..327
                     /locus_tag="ACHA02_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFM8671252.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          176 a           45 c           82 g          130 t
ORIGIN      
        1 tcaatcattg ttatataata aaatgttata taataaaatt actaatgata tttaatatta
       61 caagcaatct taagaaattc gaaaaggaaa aaatgattat gaaaaagaaa gaaaaagaaa
      121 gtattgaaac attgtgttat tctaggaata ttaggaactt gtctagctgg cattggtaca
      181 ggaatagacg ttgacgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa
      241 tgttgggtaa attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt
      301 tttggtagtt ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag
      361 tcatacatga attatacaat agtttaatga cacgacctaa tcagacgaaa gcattattag
      421 atattactga tgt
//
LOCUS       DAYYDS010000255          415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000255 DAYYDS010000000
VERSION     DAYYDS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.278"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACHA02_003010"
     CDS             complement(<1..339)
                     /locus_tag="ACHA02_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8671253.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAYYDS010000256          414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000256 DAYYDS010000000
VERSION     DAYYDS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.279"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          160 a           65 c           56 g          133 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tctt
//
LOCUS       DAYYDS010000257          345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000257 DAYYDS010000000
VERSION     DAYYDS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.280"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..321)
                     /locus_tag="ACHA02_003011"
     CDS             complement(<1..321)
                     /locus_tag="ACHA02_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8671254.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           97 a           55 c           63 g          130 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tgaat
//
LOCUS       DAYYDS010000258          343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000258 DAYYDS010000000
VERSION     DAYYDS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.281"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>343
                     /locus_tag="ACHA02_003012"
     rRNA            <1..>343
                     /locus_tag="ACHA02_003012"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          106 a           73 c          103 g           61 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acg
//
LOCUS       DAYYDS010000259          338 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000259 DAYYDS010000000
VERSION     DAYYDS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.282"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..338
                     /locus_tag="ACHA02_003013"
                     /pseudo
     CDS             1..338
                     /locus_tag="ACHA02_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612034.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DAYYDS010000260          331 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000260 DAYYDS010000000
VERSION     DAYYDS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.283"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>331
                     /locus_tag="ACHA02_003014"
     CDS             <1..>331
                     /locus_tag="ACHA02_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HFM8671255.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DAYYDS010000261          323 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000261 DAYYDS010000000
VERSION     DAYYDS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.286"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHA02_003015"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHA02_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8671256.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAYYDS010000262          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000262 DAYYDS010000000
VERSION     DAYYDS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.287"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHA02_003016"
     CDS             complement(<1..>321)
                     /locus_tag="ACHA02_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8671257.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYDS010000263          314 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000263 DAYYDS010000000
VERSION     DAYYDS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.288"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>314
                     /locus_tag="ACHA02_003017"
     CDS             <1..>314
                     /locus_tag="ACHA02_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8671258.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TY"
BASE COUNT          123 a           59 c           49 g           83 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaa
//
LOCUS       DAYYDS010000264          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000264 DAYYDS010000000
VERSION     DAYYDS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.289"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHA02_003018"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHA02_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAYYDS010000265          308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000265 DAYYDS010000000
VERSION     DAYYDS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.290"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..>308)
                     /locus_tag="ACHA02_003019"
     CDS             complement(76..>308)
                     /locus_tag="ACHA02_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8671259.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DAYYDS010000266          292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000266 DAYYDS010000000
VERSION     DAYYDS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.291"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..185
                     /locus_tag="ACHA02_003020"
     CDS             <1..185
                     /locus_tag="ACHA02_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8671260.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DAYYDS010000267          284 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000267 DAYYDS010000000
VERSION     DAYYDS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.292"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..>284
                     /locus_tag="ACHA02_003021"
     CDS             26..>284
                     /locus_tag="ACHA02_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8671261.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT          107 a           39 c           58 g           80 t
ORIGIN      
        1 gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga
       61 tgtgattatt gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca
      121 agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca
      181 agaatatggc aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc
      241 atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggc
//
LOCUS       DAYYDS010000268          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000268 DAYYDS010000000
VERSION     DAYYDS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.denovo.293"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHA02_003022"
     CDS             <1..>282
                     /locus_tag="ACHA02_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8671262.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAYYDS010000269        15429 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.guided.060, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000269 DAYYDS010000000
VERSION     DAYYDS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.guided.060"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..339
                     /locus_tag="ACHA02_003023"
     CDS             <1..339
                     /locus_tag="ACHA02_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFM8671263.1"
                     /translation="LAKQKSKKTREKVDKAISKFSIEGKVINFNSIAKEANVSKSWLY
                     KEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVMELEKENKKLQNQIQ
                     KLYGDLYNKE"
     gene            493..1275
                     /locus_tag="ACHA02_003024"
     CDS             493..1275
                     /locus_tag="ACHA02_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFM8671264.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1401..2132)
                     /gene="erm(A)"
                     /locus_tag="ACHA02_003025"
     CDS             complement(1401..2132)
                     /gene="erm(A)"
                     /locus_tag="ACHA02_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFM8671265.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(2190..2249)
                     /locus_tag="ACHA02_003026"
     CDS             complement(2190..2249)
                     /locus_tag="ACHA02_003026"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFM8671266.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            2641..3303
                     /locus_tag="ACHA02_003027"
     CDS             2641..3303
                     /locus_tag="ACHA02_003027"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8671267.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            3538..3681
                     /locus_tag="ACHA02_003028"
                     /pseudo
     CDS             3538..3681
                     /locus_tag="ACHA02_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            3831..4031
                     /locus_tag="ACHA02_003029"
     CDS             3831..4031
                     /locus_tag="ACHA02_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8671268.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(4285..5964)
                     /locus_tag="ACHA02_003030"
     CDS             complement(4285..5964)
                     /locus_tag="ACHA02_003030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8671269.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(5966..6178)
                     /locus_tag="ACHA02_003031"
     CDS             complement(5966..6178)
                     /locus_tag="ACHA02_003031"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8671270.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            6571..8301
                     /gene="efrA"
                     /locus_tag="ACHA02_003032"
     CDS             6571..8301
                     /gene="efrA"
                     /locus_tag="ACHA02_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8671271.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            8298..10058
                     /gene="efrB"
                     /locus_tag="ACHA02_003033"
     CDS             8298..10058
                     /gene="efrB"
                     /locus_tag="ACHA02_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8671272.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            10153..10350
                     /locus_tag="ACHA02_003034"
     CDS             10153..10350
                     /locus_tag="ACHA02_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671273.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            10503..10988
                     /locus_tag="ACHA02_003035"
     CDS             10503..10988
                     /locus_tag="ACHA02_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8671274.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(11092..11685)
                     /gene="clpP"
                     /locus_tag="ACHA02_003036"
     CDS             complement(11092..11685)
                     /gene="clpP"
                     /locus_tag="ACHA02_003036"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8671275.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            11865..12392
                     /locus_tag="ACHA02_003037"
     CDS             11865..12392
                     /locus_tag="ACHA02_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671276.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            12483..13220
                     /locus_tag="ACHA02_003038"
     CDS             12483..13220
                     /locus_tag="ACHA02_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8671277.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            13224..14141
                     /gene="miaA"
                     /locus_tag="ACHA02_003039"
     CDS             13224..14141
                     /gene="miaA"
                     /locus_tag="ACHA02_003039"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8671278.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            14143..15372
                     /gene="hflX"
                     /locus_tag="ACHA02_003040"
     CDS             14143..15372
                     /gene="hflX"
                     /locus_tag="ACHA02_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8671279.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5030 a         2575 c         3213 g         4611 t
ORIGIN      
        1 ttggcgaagc agaaatcaaa aaagacaagg gaaaaagtag acaaagcgat ttctaaattt
       61 tcgattgaag gtaaagttat taattttaat tcaatagcaa aggaagctaa tgtttctaaa
      121 tcatggcttt ataaggaaca cgatattagg caaagaatcg aatcccttcg tgagcgtcaa
      181 ataacagcaa atgtagtctc aaaacccaag aaaagttctc gttcggagga aatccttatt
      241 aaaaccttaa aaagaagagt aatggaatta gaaaaagaaa ataaaaaatt acagaaccaa
      301 attcaaaaat tatatggaga tctgtataat aaagaataat tattaatctg tagacaaatt
      361 gtgaaaggat gtacttaaac gctaacggtc agctttattg aacagtcatt taggtatatg
      421 tccaatctag ggtaagtaaa ttgagtatca atataaactt tatatgaaca taatcagtta
      481 cgaggtgaaa tcatgagcaa tttgattaac ggaaaaatac caaatcaagc gattcaaaca
      541 ttaaaaatcg taaaagattt atttggaagt tcaatagttg gagtatatct atttggttca
      601 gcagtaaatg gtggtttacg cattaacagc gatgtagatg ttctagtcgt cgtgaatcat
      661 agtttacctc aattaactcg aaaaaaacta acagaaagac taatgactat atcaggaaag
      721 attggaaata cggattctgt tagaccactt gaagttacgg ttataaatag gagtgaagtt
      781 gtcccttggc aatatcctcc aaaaagagaa tttatatacg gtgagtggct caggggtgaa
      841 tttgagaatg gacaaattca ggaaccaagc tatgatcctg atttggctat tgttttagca
      901 caagcaagaa agaatagtat ttctctattt ggtcctgatt cttcaagtat acttgtctcc
      961 gtacctttga cagatattcg aagagcaatt aaggattctt tgccagaact aattgagggg
     1021 ataaaaggtg atgagcgtaa tgtaatttta accctagctc gaatgtggca aacagtgact
     1081 actggtgaaa ttacctcgaa agatgtcgct gcagaatggg ctatacctct tttacctaaa
     1141 gagcatgtaa ctttactgga tatagctaga aaaggctatc ggggagagtg tgatgataag
     1201 tgggaaggac tatattcaaa ggtgaaagca ctcgttaagt atatgaaaaa ttctatagaa
     1261 acttctctca attaggctaa ttttattgca ataacaggtg cttactttta aaactactga
     1321 tttattgata aatattgaac aatttttggg aagaataaag cgtcctcttg tgaaattaga
     1381 gaacgcttta ttactttaat ttagtgaaac aatttgtaac tattgaaaat agaaagaaat
     1441 tgttccttcg atagtttatt aatattagtg acatttgcat gcttcaaagc ctgtcggaat
     1501 tggtttttag tgaaaagaac acgatattca cggtttaccc acttataaac aaaagatcga
     1561 tactttttgt agtccttctt tgaaatcaat ggttgatgtc gttcaagaac aatcaataca
     1621 gagtctacac ttggcttagg atgaaaatat agtggtggta cttttttgag catttttata
     1681 tccatctcca ccattaatag taaacccaaa gctcgttgca gattttgcaa tcttttcgca
     1741 aatcccttct caacgataag atagctatat ttagcctgac tttcaaaggt aattcttttg
     1801 acaatatccg tactgatgtt ataaggaata ttaccatata tcttatagtt tatatgtttt
     1861 gggaaggaaa attttagaat atccgtttga atcactttta tattctcaga ggggtttacc
     1921 gcttctttag tcacttgaca taagcctcca tcaatttcta tagcagtaac tgatcgactc
     1981 attttgacta gctctttggt aaaatgtcct tttcctgatc cgatttctat tacgttgtct
     2041 tgtttactga tattcgtgtg attcaatatt tcttttacat gctttttaga agtaataaaa
     2101 ttttgcgtgt ctttagggtt tttctggttc attataacct tctccttact ggttataatg
     2161 aactgacatt aagcgttcat tataaccaat taattttgat ttggttgata acgaacttta
     2221 ttaataacaa atatagaaaa agtacccata cttttatctc ctttccattt ttttcattat
     2281 gaatgagata aagtaatttc tactactgcg atactggtgc acataattga aacctcctta
     2341 tgtaactgaa tatgattgta tcatcttttc attttagaat aaacctttat atgtaatttt
     2401 gcacttgaca cttcagatgg acgttttctt caaaataata tgttttgaaa cttgataaga
     2461 gaagccactg tctttactaa aaagtaaagg ctgcgataaa ggacagtgac tttaataaag
     2521 ttgcactgtt gtattgtctt tggataaaat tgtctaaagg gaagttttat ccccaacgaa
     2581 aaaacatcta aagtggtaag taatgaaaga atgtcgataa agtaggaatg ggggatcttt
     2641 ttgaatacaa tagaatcaca gtttgataag gttgcagaag attacgattt cgtgaatgag
     2701 cttttgaatg attattcatt ctttgtgtct aatatgtctc caaagaaagg cagagcatta
     2761 gatatcggat gtggctcggg tttgttagtg gagaaattag caagttatta tgatgaagtg
     2821 gtagggattg atatttctaa tcaaatgctc gatcttgcca aatctaaacg tcaactaaca
     2881 aatacggtct atctgaatat gaatgcagaa caacttaatt ttaatgagaa gtttgatttt
     2941 attgtaagcc gaacaacctt tcatcatttg gatgatatag ccagcgtaat acagcaaatg
     3001 aaggaactgt tgaatgagga aggaagaata gttattcttg ataatgtatc tgaagtcgag
     3061 acaccgccta cctatgttta taaattaggg gcaatccaag agtttttacc gcattgtttc
     3121 aaatttggaa taaaaaatgc gattagaatt tataatcaca acacatctaa atcatggctt
     3181 gagcatttag cttcagataa gtacctatct gaacaaaatt attacgattt atatgaaaag
     3241 ttgttgcctg gatgccaatt ccataaaatg ggttgggcaa tgggggttgt ctggacaaaa
     3301 taacttaaag ctgtggcttt gaaaagttga atttgtgatt gttttcagag ctatcgggtg
     3361 ctttagtata aataacaatg aataaaaaat cagcctgtat cttatgttac tattaagtaa
     3421 tttaagatac aggttttact atttttgaaa taagtacctt ccacttcaaa tacatgtatt
     3481 caaagtgaaa gtattgattt aaatgattct tttgtaattt actcttgata atatgaaatt
     3541 ccatcgcagc aggatatgaa ttttaccaaa aggatacaaa agtgtggaga aatgatgggg
     3601 atcactgtac tggaccactt gatcatcgga cgcaaacgat attttagttt gagagaagaa
     3661 ggaatgatgg aagagaagta agttgctttc ttgcaaaaaa aatgaagcaa gtgtacaatt
     3721 atattgtaat tttgtttggg aatagaagtt gaaacatctg ttgcacctct atcgtacacg
     3781 agcaatgtta caatttaaat tagtgctttt ggcacgagga ggatattatt atggaaaacg
     3841 gaacagtaaa atggttcaac gctgaaaaag gatttggatt tatctcacgt gaagatggaa
     3901 gcgacgtatt cgttcacttc tcagctatcc aaggtgacgg attcaaaaca ttagaagaag
     3961 gtcaagcagt gactttcgac gttgaagaat cagaccgcgg cccacaagct gcaaacgttg
     4021 taaaagctta attttatctg taaatcaaat tgatggtaga catcaatttt tacgagagaa
     4081 caaagcgagt aacaaaaata cctgccacaa gagacaattt ctcttgtggc aggtattttt
     4141 ttgtatgaaa aagtaaataa atttaagagg ctatgaaaga gccaccttgt atcaaggaat
     4201 aagaacaaac aaaaatggct tctcatgttt ttcatttgtt ttctcttata ttcaggacaa
     4261 ggtgctgatt tcataacctc ttatttattc ttcatctggt gtcattacca ttggcaaaat
     4321 cattggatgt ctttctgtac gttcgaataa gaaaggctga agcgcgccag tcatcgcttc
     4381 tttcaattta tattctgaac agtcaggtcc attcaaacat tctcttaaag cgtggaataa
     4441 gattcgctga gcttcattga tcatttctcc tgattctcgc atgtagacaa atccacgaga
     4501 gagaatatct ggaccagcca agatttcttt tttcttgata tccactgtcg cgacagctaa
     4561 tacaagacct tcttctgaaa gcaggtgacg gtcacgtaaa acgacattac caatatcacc
     4621 gatcccgcta ccatcgatat acacatctcc agccacaaaa tgcccagcag gacgcgcaga
     4681 atcgctagtt aacgctaaaa catcgccatt ttccatgatg aagcagtttt ctgcaggaac
     4741 acctacatct tgtgctagag aagcatgtac tttcaacatg cggaattctc cgtggacagg
     4801 aataaaatat tttggcttca tcaaacgaag cattaatttt tgttcttctt gtccaccgtg
     4861 accggaagta tggatgttat tgatttttcc gtgaatcact tctgcaccag cttcagacag
     4921 caaattgatc aaatgattga cacttgttgt attaccagga ataggtgaac ttgaaaagat
     4981 cactgtatcg tctggctgga tttggatttg acggtgagta ccattcgcga tgcggctcaa
     5041 cgcggccatc gcttctcctt gagaacctgt acataggatc atcgtttcat tggcaggcaa
     5101 gcgattgatt tcgtgtgcat ctacaaatgt gccgtctgga actttgatat atcctaaacg
     5161 ttgaccgttg acgatggcat tttccatgct tcgtccgaaa acagcgattt tacgaccagt
     5221 tgccacagcg gcatctgctg cttgctgcaa acggaagatg ttagaagcaa agctggcaaa
     5281 aatgatacgt ccttcgattt ttgaaaagat cttcaaaata gaatgcccaa tcgttttttc
     5341 tgatttagta aatgttggaa tctcagcatt tgtactatca gataaaaggc acaagactcc
     5401 ttcttccccg atacgagcca ttttatgcaa gtttgctggt tcgccaacag gagtgaaatc
     5461 gaatttgaag tctccagtcg ctacgatatt tccagaaggt gttttcacca cgatccctaa
     5521 tgcatctggg atactatggg tcgttctgaa aaagctgaca gaagtctttc taaaacggat
     5581 gatcgtatct tcattgatct catggagctc tgcatctcga agcaagccat gctcatctaa
     5641 tttgtttgtg atcaacgcta atgcgagagg accagcatag ataggaatat ttgcttgttt
     5701 cagcaagtag ggtattccgc cgatgtggtc ttcgtgcccg tgagtaatca ccaaggcttt
     5761 tactttgtgt aagttttgta tgatgtagct gtagtcggga atgacatagt caattccaag
     5821 taaatcgtct tccgggaatt tgatcccggc atcaatcaaa atgatttcat cttggaattg
     5881 taccccatac gtatttttcc cgatttcacc taatccacca atagcaaaga cgcctgtctc
     5941 gttatttttg atggaaagtt tcatattaaa actccactaa tgagaaatca gcattttctt
     6001 tttcgtactc tagatgactt ccttctagaa gttgaacgaa ttcaatattg tatggggtat
     6061 tttcttccac acgttggcga gcgtctacat cgctttttgc ctcaatgtaa agagattttg
     6121 tttcttcccg tttagggttg cgtacttttg tttcttgata ataaacttta tagatcattg
     6181 ttttgtctcc tttatctgcg taaagcagtg atttagctag ttgatttttt tagacaacac
     6241 aaaacgaggg gacaagccac ctggtttttc atatgctttg actagaatac tggattcgag
     6301 tcaaagagac aagcctcgtt tttgcttatc tgttcggata ttttttcaac cgtaccttct
     6361 gtataaaaaa tatacaacaa ttaattgtta ttctagcata aaacagcaaa taagtataga
     6421 aatcaactag attgaaagaa aaaatcaatg tttatcatcc cgctataatc ataatgactt
     6481 ttttatgaga gaagtgttct attaataggc tcgttgtgct atcattttta gtaatgaaaa
     6541 tgtaacaaaa gattaaaagg agatgagaaa gtgaaactaa tgtggcatta taccatgcgt
     6601 tataagaaat tcttgttttt caatttcata tgcgtatttg gttttatttt gattgaatta
     6661 ggacttccta cgattttagc acgtatgatc gatgtaggaa tccgcaatga tgattttgat
     6721 tatgtgaagc aacaagggat atttatgatc gttattactg taatcggtat tattttgaat
     6781 atcttgttag gttattttgg ttcgaggatt acgactaata tcgtagcaga tattcgagat
     6841 gatttgttcg aaaagatcca aagttattcg catcaggaat acgaaaatct aggcgtatct
     6901 tcattgatca ctcgtacaac aaacgatgct tatcagatca tgttgttcct gcaaaatatt
     6961 ttacgtatcg gttttatggc accaatgatg ttcattgtca gtttgtacat ggtcatgcgt
     7021 acaagtgtga cactcagttt gtatgtagta ggtgcattac ctttactatt attggcagtc
     7081 gtagcgattg ctaaggtgtc tgaaccgtta tctaaaaagc aacaaaaaaa cttagataaa
     7141 atcaacagta tcttgcggga aaatctttca ggattgcgtg tgattcgtgc attcgtcaat
     7201 gaaaagtttg aagaaaaacg ttttgctgta gtcaatgaga attatgcttc tagttcaaaa
     7261 agcttattcc gtttgatggc tgttgcacaa ccgggttttt atttcttatt taatatcgta
     7321 atggtattga tcatctggag cggggcagtg caaatcgatg caggcgagct tcgtgtagga
     7381 gatttgattg cgtttatcga gtatatattc catgccttgt tctcattcat gttatttgct
     7441 agtgtgttca tgatgtatcc aagagcagct gtttcagctg gacgtatcca agaagcccta
     7501 gatgccaaac cttcgattgt agaagatcca aatggtatat ctgatacaaa aactaaaggg
     7561 tatatcgaat ttcaaaatgt aacatttgct tatccaggcc attcgcaaga gccagtgata
     7621 cgaaatgtca gttttacagc ctctccagga gagaccgttg cttttattgg aagtaccggt
     7681 agcggaaaat ctacgttgat ccaattgatc cctcgctttt atgatgtgag tgaaggaaag
     7741 atcttgatcg atggagtaga cgtgagagat tatcggctaa gcaagttgcg agataagatt
     7801 gggtttattc cgcaaaaagc gctgctattt actggaacga ttgcagataa tttgcgatac
     7861 gggaaagaag atgctacgca agaagaaatg gaacgtgcag ctgagattgc tcaagcttca
     7921 gaattcattt ctcgaaaacc agatggattt aatgaacatt tatcagaagg tggttccaat
     7981 ttttcaggtg gacaaaaaca acgattggca atagccagag ccatcatacg ccgtccggaa
     8041 atctatattt ttgatgacag cttttctgca ttagattatc aaacggatgc gaagttacgt
     8101 gctcgtctga aaaaagaaac gacagaatct actgtattga tcgtagcaca acgtgtggga
     8161 acgatcatgc atgcagataa gatcatcgta ttaaatgaag gagaagtcgt cggtatgggg
     8221 acgcatcggg agttgttgga aaaatgcccg atttattatg atattgccgc ttctcaatta
     8281 tcaaaggagg aattagcatg aaaaaaacct tttcttcctt aaaacgttta gctcgatata
     8341 tcagaccgta tcgtgagact tttcttttag tgatcctatt tacagttctg acagtggctt
     8401 tcaacgtggc tttgccatat gttacaggac ttcctactac agaaatcagc cgtaatattg
     8461 ccaatggcga atcaatcaat ttttcttata tattcaaatg tctcatttgg atcacagttg
     8521 ttggaatagg gtattgtatt tcgcaattac tttctggtgt tttgatgaca aatgtcgttc
     8581 aaagtgccat gcatgatttg agaagagaca tagaagaaaa aatcaatcgt cttccggttt
     8641 catatttcga taaaaaccag caaggaaata ttttgtctcg tgtgacgaat gatgtagatg
     8701 cagtcagcgg tgctttacaa caagctttta ttggtatcgt aaatgcgata ttgggaatta
     8761 cgttggctgc agcaatgatg ttttatatcc agccgatgat ggcgctgatc tctatgatca
     8821 tgattccaac atcgatctgg atctcaaaac gagtgatcaa tgcttcgcag aaatattttc
     8881 aaagcatgca gaattctctt ggtgagctaa acggctacgt ccaagaaaat atgacaggtt
     8941 ttagtgtatt gaaagtatat ggacgcgaaa aagaaacatt ggaaggattc aaaaaagtta
     9001 atcacagctt gaaatacttt gggtttcgtg cagcattcat ttctggcttg atgatgcctc
     9061 tagttcaatt aactgcctat gctacttata ttggtatggc tgttctagga agcttttatg
     9121 ttattactgg ggcgatcgtt gtcggtcaac tacaagcatt catccaatat atttggcaaa
     9181 tcagtcagcc aatggggaac gtgacacaat tgtcttcagt tttgcaaagt gcatcagcgg
     9241 caacgaaacg tgtattcgag attttagatg agccagaaga aaaagaaaac aaagtagatg
     9301 cttcactgcc agaaacgatt ttaggagatg tgacattcga tcatgtcgat tttgcatatg
     9361 atccgaagaa accattaatc caagatttga attttgaagt gaaagctggt caaaccgtag
     9421 ctgtggtcgg tccgaccggt gccggcaaga cgacattgat caatctattg atgcgttttt
     9481 atgacgtaga caaaggagca ataaggattg atggaatcga caccaaatca atgagtcgca
     9541 gtgatgtgcg ttcgctattt ggtatggtct tgcaagatgc atggctttat caaggaacga
     9601 ttgcagataa tatccgcttc ggaaagcttg atgcgacaga ttatgaagta gttgatgctg
     9661 cgaaaacagc aaacgtagat cattttatcc gaacgatgcc agatggatat gatatggcca
     9721 tcaattctga gggagacaat gtgtctttag gacaaaaaca gttgctgacg attgcccgag
     9781 cagtggtctc cgatcctaaa atcttgattt tagatgaagc aacaagttca gtagacaccc
     9841 gtttagaagc gttgattcaa aaagcaatgg atcgcgtgat ggagggacgg acaagtttcg
     9901 tcatcgctca ccgtttatcg acaatccgtg atgcagatct gattctagtg atggatcaag
     9961 gtcagatcat tgaaaaagga acgcatgaga gtttattggc acaaggtgga ttttatgaaa
    10021 aactgtataa cagccaattt gctgaggaag cagaataagt catataagaa taaaacagtt
    10081 taaaacttat tttttgttct ctattcttga taatggtaaa gtaaagttat caagaaaagt
    10141 gaggacacgg agatggaaca gtttttaaat gaaaaaatta ctgaagccaa aagagaagtt
    10201 gatcgtttaa cgcacgtctg tcagacgaag ccttttgatt cagctgattt ttttgaaaat
    10261 gagtttcaaa tcgaacgctt ggcagcacaa atagaagtgt acgaattaat tttaaaaaaa
    10321 gtaatagaag aaaatataga tgtacaataa aaacaggtaa aacgtcagtt accagatact
    10381 gtaaatagac atctggcctg agcaggtttt acctgttttt tttcttatga aataaatata
    10441 tgctatgata cctttattca ataggattag tttctacaaa aaatgaaagg agaaaaataa
    10501 atatggaacg gtttatagca tcaaatgcca cagtgattgg tgatgtgaca ttgtcagaag
    10561 atgtgacgat ctggtatcaa gctgttttac gcggggacag taattggata aaaataggac
    10621 aaagaacgaa tatccaagat ggtacaatca tccatgtcga tcatgatgca cctgtagata
    10681 ttgcggagaa tgtgacggta ggacatcagt gcatgctaca tggctgtaca atcgaaaaag
    10741 gggcgctgat cgggatgggg accacgatat tgaatcatgc ggttattggt gagaatagtt
    10801 tgattggtgc tggatcatta gtaacagaag gaaaagttat tcctcctaat gtcttggctt
    10861 ttggtcgccc agctaaagtg atccgtcctt tgacaaaaga agaaatacaa aaaaacaaag
    10921 aaaacatcca gcattatgtc gagataggtc ggatgcatgc gcaaggaaaa ttcaaggagc
    10981 aaagataggc agtagcatcc ataaaataaa aaaggatggt cagtttgagt tgaccatcct
    11041 tttttatttg gtttagataa gagagcttaa aaaagcttcc tttgaaacag attaatttaa
    11101 tgaagaactg ttttccatga cttcatcgat caatccatat gcttttgctt gttcagctgt
    11161 catatagttg tcacgatctg tatctttttc aatcacttca agcggttgtc cagtgcgttc
    11221 agctaaaatt ttgtttaagc gttcgcgtgt ttgcaaaata tggcgtgccg caatttcgat
    11281 ttcagttgct tgcccttgag cgccgccaag aggttgatgg atcatgatct cagcatttgg
    11341 taaagcaaat cgtttacctt ttgtaccagc tgtcagcaag aagcttccca ttgaagcagc
    11401 catacccata acaattgttt ggacatctgc tttgacaaag ttcattgtgt cgtaaatagc
    11461 catacctgct gtaactgaac caccaggaga attgatataa agatagatat ctttttcaga
    11521 atcttgagcg tctaagaaaa gcaattgggc gatgatcgaa ttagcaagat catctgttac
    11581 ttggccgctt aacatgatga tacggtcttt caataaacgt gagtagatat cgtaagctct
    11641 ttcacctcgt gaagattgtt caatgactgt aggaattaaa ttcataatag agttcctcct
    11701 tgatttcgtt tagaaatagt gtaccataat ttagtttcta atacctaaaa tgtagtatac
    11761 atttttggtc aaaaaaggtc aaataaaaaa cttaggcaaa ttagtcgcat aaacccgctt
    11821 tatggtatga taagcagaga aacaaaacaa ggaggaacaa cagattgata gttggtatta
    11881 ctggccttat cggagtgctg acaggtgctg tagttgtcta tatcggcatg atactgcaaa
    11941 tgcgtcactt ggaaaataaa gaacagcgta gacgtgaatt ggaattgaaa gtcaaagaaa
    12001 tcgagacgct gaatcaatta aataaaaaga tcaacgagat cttgcaaaaa cggacaacgc
    12061 tgatggaaga atatgtcagc tttgatgcat ttgatgattg ttatatcacg attgacgatt
    12121 ttgcgtatct ccaatcattt tcagcgcaaa atagttttta tctgccaaat tatattttag
    12181 acgagctatt taagaatatc tcccatcgaa aagtgatttt atctccagat gaaacaatga
    12241 agattggtgg atatgcttat aaaggcggac gcgtgatttt agagaatttt acggatagtc
    12301 tgactgaaat gatcaatgaa aaaaaagctc agttgaagac tatgacgcgc cagccgattg
    12361 gtttcttctc gaaagatgat gtttggagct gatattttga agcattgccg ttgcacatga
    12421 gagcgtggta atgctttctt tttttcttaa tgatacaata gaactgaacg gagtgaagaa
    12481 agatgttaca aatctatgcc catcgcggaa gcagcggcac ccacccagaa aatacgcttc
    12541 ctgcatttgc agaagctgta cgagtaggtg cagatggtat cgaactagat gtccatctaa
    12601 gcaaagatgg ttatttgatc gttatgcacg acgaagaagt ggaccggacg actaacggta
    12661 aagggctgat tcgagaaaaa acattggaag aactcaaaaa attgaatgca gggggcaaat
    12721 tcagcaaaga atttccagca gctaaagttc ctgaattgag agaagtgtta ggactgctcc
    12781 tccaaaaaaa ttaccgaggg atgctaatga ttgaaattaa aactgatcag tatgaatatc
    12841 ccggtatcga agaaaaatta gccgaaacat taaaaagtag agattggcct tttcaacatg
    12901 catattgcag ctttcatttg gattctttgg aaaaaatcgc attactggaa ccggagatac
    12961 agctggactt attgatgagt acttctgata aaaagatcca actagcagca gaaacccctt
    13021 atatagaagg agtacatcca aaaaatactt ggttgttcga acatccagtc caggcagctg
    13081 attttccaaa agcaattcgt gcatggacga ttaacgatga agaagaaatg gccaaatgtc
    13141 aagaactcaa aatagatgga ataataactg attttccgga gaaagctctt cggtggaaac
    13201 aatcaacgaa aagtaggtaa aatatggaaa aagtattagt gatcgtcggt ccgacagcag
    13261 tcggtaaaac agcgctaagt gtggaactcg cacaactatt tggaggagag atcatcagtg
    13321 gtgattcatt gcaaatctat aaaaatctgg atatcggtac agcaaaaatc aaagaagaag
    13381 agatgaataa tgttccgcat catttgattg atgtgatcga acctactcaa cgttactcag
    13441 ccgctgattt tcagcattca ggtcgacaat tgattaccga gattgcgaac cgagggcacc
    13501 tacctatcat agctggtggt acgggtctct atatccaatc ccttctatat gattatcagt
    13561 taggagcgac agaagaagcg aatcctgaga ttagaaaaaa gtatgaaacg ttagctgaaa
    13621 caattggttc ccaagcatta tggaatcagt tgaaagaaaa agacccggca gctgctgaga
    13681 aaatccactg gaacaatcaa cgcaaagtga taagagctct ggaagtttta gagacgacag
    13741 gacatagtat cacggcacct aaagaagagc caaagcaatt gtatgattac ctcatgattg
    13801 gattagatac gaagcgtagt attctttatg aacgaatcaa tcaaagagtc gatttgatgc
    13861 tagcagaagg tttagtagca gaagcgcgta aggtgtatga gcttggagaa gtccaggcca
    13921 gtcaaggtat tggatataaa gaatttttcc cttattttga agggaaagaa acattggaag
    13981 aagcaacaga acaagtcaaa ctacattcga gaagatacgc gaaacgtcag ttgacgtggt
    14041 ttcgtaaccg tttggatgca cagtggtttg atcttataag tgagccaaat caaaaaagtc
    14101 agattgagca aacaatcaaa aaatggctgg aggcggaata aaatggaaga aaaagtgatc
    14161 atcgtagggg tagaaaccga agcaaaccag cgttactttt cagagtcaat ggaagagcta
    14221 gttcagctga cgaataccgc atcaggagaa gtcgtattca caattacaca aaagagacca
    14281 caagtggatc ggcaaacgat tatcggtaaa gggaaattac aagaattagt tcaacaagcg
    14341 gatgcgcatg aagcggattt gatcatcttt aatcatgagc taactccgag acagagccaa
    14401 ctgattaccg atgctgttgg tcttccggta attgatcgcg tgcagcttat cttagatatt
    14461 tttgcgatgc gtgctcgttc gaaagaaggg aagctgcagg ttgaacttgc tcagttagaa
    14521 tacctgctgc ctcgtttagt agggcaaggg aaaacattat ctcgtcttgg cggtgggatc
    14581 ggaacaagag ggccaggtga aacaaagtta gaaacagacc gccgacatat acggaataaa
    14641 atttttgctg ttaagaaaga attgaaagaa gtagaagctc atcgagagcg caatcgccaa
    14701 aaaagaaaaa atagcgagat ttttcagatt ggacttattg gctacacgaa tgccgggaag
    14761 tcaactatct tgaatctatt gacacaagcg gatacttatt cgaaagatca attgtttgca
    14821 acgttagatc cattgactaa gagatggcgt tttgctgaag gatttgagat tactgtaacg
    14881 gataccgtcg ggttcatcca agacctgcct acgcaactga tcgatgcttt ccattctaca
    14941 ttagaagaaa gtcaaaatat ggatcttttg ctacatgtag tcgatgctag ttctccagat
    15001 cgtattttac aggagcagac tgtcttaaaa ttgatggatg aactgaatat gaaagaaatg
    15061 ccgatactga ctgtttacaa taaggcagac cagatcgacc cagcgatgtt cacgcctacg
    15121 ctattcccaa atgtattgat ttctactcag acgaaagaag gtaagatggc gttgatcgaa
    15181 gcaatcaaac gtcagctgat ggaactaatg accccttata cactttctgt gccaagcaac
    15241 gaaggacaga aattaagcga gttgagaaga caaacaatgg ttttggacga agcgtacatg
    15301 gaagaaacaa acgaatatga agttaaaggg tttgcaaaaa ccaattcaaa atggttaaga
    15361 aaaaaccaat aaaattttat ttttttaaca atattatttg tcaaattaag cgagacaaaa
    15421 ctttccatt
//
LOCUS       DAYYDS010000270        13074 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.guided.074, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000270 DAYYDS010000000
VERSION     DAYYDS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.guided.074"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..215
                     /locus_tag="ACHA02_003041"
     CDS             <1..215
                     /locus_tag="ACHA02_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFM8671280.1"
                     /translation="CFSVRRNPLKGNGELGKRSPFNDNKTRLYRPSLYERLGSDTNLS
                     FRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            287..682
                     /locus_tag="ACHA02_003042"
     CDS             287..682
                     /locus_tag="ACHA02_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8671281.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            666..3119
                     /locus_tag="ACHA02_003043"
     CDS             666..3119
                     /locus_tag="ACHA02_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8671282.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            3116..5143
                     /locus_tag="ACHA02_003044"
     CDS             3116..5143
                     /locus_tag="ACHA02_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8671283.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLIQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            5140..6162
                     /locus_tag="ACHA02_003045"
     CDS             5140..6162
                     /locus_tag="ACHA02_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM8671284.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            6179..7108
                     /locus_tag="ACHA02_003046"
     CDS             6179..7108
                     /locus_tag="ACHA02_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8671285.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            7422..7469
                     /locus_tag="ACHA02_003047"
                     /pseudo
     CDS             7422..7469
                     /locus_tag="ACHA02_003047"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7485..8285
                     /locus_tag="ACHA02_003048"
                     /pseudo
     CDS             7485..8285
                     /locus_tag="ACHA02_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            8523..8759
                     /locus_tag="ACHA02_003049"
     CDS             8523..8759
                     /locus_tag="ACHA02_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671286.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            8766..10718
                     /locus_tag="ACHA02_003050"
     CDS             8766..10718
                     /locus_tag="ACHA02_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8671287.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(10790..11287)
                     /gene="dfrG"
                     /locus_tag="ACHA02_003051"
     CDS             complement(10790..11287)
                     /gene="dfrG"
                     /locus_tag="ACHA02_003051"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8671288.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            11562..12689
                     /locus_tag="ACHA02_003052"
                     /pseudo
     CDS             11562..12689
                     /locus_tag="ACHA02_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            12787..12975
                     /locus_tag="ACHA02_003053"
     CDS             12787..12975
                     /locus_tag="ACHA02_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM8671289.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
BASE COUNT         4447 a         2198 c         2514 g         3915 t
ORIGIN      
        1 attgtttttc agttagaaga aatcctttaa agggaaatgg tgagctggga aaacgaagtc
       61 cttttaatga caataaaacg agattgtata gacccagctt atatgaacga ttagggtcag
      121 ataccaattt aagtttcaga tattcaatga atccagatga tgaagaaact gatgaaggta
      181 ttattgcaaa atggacaaaa aataaaattg aatagttaaa gcattgaaag agcaatcacc
      241 tattttgatt gttctttttc tatttttacg gaaaggacgt gaagacatga aaaaaataag
      301 aagttacacc agtatttggt cagtagaaaa agtgatttat gcgattaatg atttacaact
      361 ccctttccca attaccttta cgcaaatgac atggtttatc gtttctttgt ttgtcgtcat
      421 actctttggc agtttaccac cactttctct gattgacgga gcactactca aatacttagg
      481 gattcccatt gttctcactg ggttcatgtg ccaaaagacc tttgacggaa agaaacccta
      541 tggatttcta aagtctatca ttacctatgc tctacgccct aaagtaacct acgctggcaa
      601 acaaatacga ttaaaaaaag aaacaatgaa tgaatcgatt acgattgtaa ggagtgaaca
      661 actagatggc atatccgatt aaatatatgg aagataatct cgtatttaat catgatggcg
      721 aagtattcgc ttattatgaa ttaattccct ataattacag ttttttaagt tccgatgaaa
      781 aaattcaagt tcatgataat ttctgtcaat tgattgccca aaatcgtgat ggcaagattc
      841 atgctctgca actagcaacc gaatcaagta ttcgagccac acaggaacaa tcgaaaaaag
      901 aaatcactgg acgattaaaa gagatagctt acgaaaaaat agatgaacag accgaagcgt
      961 taatttctat gattggtgac aatcaagtag actatcgctt ttttattggt tttaaactct
     1021 acctcaatga acaggaagta tccgtaaaaa atgttgggaa agaaattcaa aaagcatttg
     1081 cggaattctt tcatggtgtg aatcacaaat taatgggcga ttttgtttct atgcctatgg
     1141 cggaaatcga acgcttttct aagatggaac agttgttaga aagtaaatta tccagacgct
     1201 ttcagattcg taaattggag aaagatgatt ttggttatat tattgaacat ctttacgggc
     1261 aaacaggtgt cccttttgat gactacgact accatttacc gaaacgatac gaagataaaa
     1321 aagtgttggt aaagaaatac gatattctca aacccactcg ctgtttaatc gaagaaaatc
     1381 aacgctattt acggattgaa caggaagata ccactactta tgtggcgtat tttacaatta
     1441 atagtattgt tggtgaattg gatttcccta atagtgaaat tttttattac cagcaacagc
     1501 agtttacttt ccccgttgat accagtatga atgttgaaat tgtcgccaat aaaaaagcct
     1561 tatctaccgt tcgcaataaa aagaaagaac tgaaagattt agatagccac gcatgggaaa
     1621 gcgacaacga aaccagtact aatgtggtgg acgctttaga atctgtcgat gaattagaaa
     1681 gtaatttaga ccaaaccaaa gaatctatgt acaaactaag ctatgtggta cgggtatcag
     1741 caaagaattt ggacgagctg aaacaacgtt gtgatgaagt aaaagatttt tatgatgatc
     1801 tgtctatcaa attagtacga ccttttggcg atatgctagg cttgcataat gaatttattc
     1861 cctcaagcaa gcgatatatc aatgactata ttcaatatgt cacgtcggat tttttggctg
     1921 gtcttggttt cggtgctaca caaatgattg gtgaaaattc tggtatttac gtcggttaca
     1981 atctcgatac aggcagaaat atttatctcc aacctagcct tgcaagtcaa ggaattaaag
     2041 gctctgtaac aaatgcatta gcgtccgctt ttattggttc attaggtggc ggaaaatcat
     2101 tctctaacaa tatgctggtc tattattcag tactctttgg cggacaggca accattgttg
     2161 acccaaaagc tgaacgtggt gaatggaaag aaaccttacc tgatattgcc catgaaatca
     2221 atattgttaa tctcacgagt gagaacgaca ataaagggtt acttgaccct tatgtgattt
     2281 taaaactccc gaaagattca gaaagccttg ccattgatat tctcacgttc ttaacaggta
     2341 tttccagccg agatagcgag aagtttcctg tcctgcgtaa agctattcgg agtgtaactc
     2401 aaagcaaaca acgagggttg ttattggtga ttgatgaatt gcgaaaagaa gatacgccca
     2461 tcagcaatag tattgctgac catatcgaaa gctttgtcga ttatgatttt gcccatctgt
     2521 tattcagtga tggcagtgtc acccaatcca ttagcttgga aaaacaactc aatattattc
     2581 aagtggcaga tttggtctta cccgattcca atagctcttt tgaagaatac actaccatgg
     2641 aattattaag tgtggcaatg ctgattgtca ttagtacctt tgctttagac tttatccact
     2701 ctgaccgttc catttttaaa attgttgacc ttgatgaggc ttggagtttt ttacaagtgg
     2761 cacaaggaaa agctctatct atgcgattgg ttcgagcagg acgtgccatg aatgcagggg
     2821 tctatttcgt tactcaaaac gcggacgatt tactagatga aaagatgaag aataatattg
     2881 gcttgaaatt tgcctttcgt tccactgacc tagtggaaat caaaaagacc ttagaatttt
     2941 ttggtgtgga caaggaagat gaaaacaatc agaaacgctt acgtgaatta gaaaatggac
     3001 aatgtttagt ttcagacatc tatggtcgtg taggggttat gcagtttcac ccgatttttg
     3061 aagagttgct tcatgccttt gataccagac cgcctgtgcg aaatgaggtg gaagaatgac
     3121 acaaaaacaa aagaaatttc ttcactatag ttggataacg ctcctttgtg tgggcgttat
     3181 tttactttta ttaggcacgc tgggcaatgc agtacaagcg acaggcttag ttgatgaaac
     3241 cattgataca agcaatgaat actccaagta tggtttgaat caatatcagc ttgattatta
     3301 tgtggacaat agctggggct ggcttccatg gaattggagt gatggcatag gacaatcggt
     3361 gatgtatgga ttgtatgcca ttactaattt tatttggaca atttctcttt atctatcgaa
     3421 cgccacaggt tatttggtga aagaagccta tgccttagat ttcattagtc aaacatccca
     3481 agcaattggc gaaaacatgc agacgattgc aggcattaca ccgaatggct tttccagttc
     3541 agggttttat gtcggtttcc tccttctttt tattctcatt gttggaatct atgttgccta
     3601 tacaggattg gttaagcgag aaacgacgaa agtgatacga gctgttttaa actttgtcgt
     3661 ggtgtttatt ctatcggctt cctttattgc gtatgcaccc gattatattg cgaaaatcaa
     3721 tgatttttcc aaagatgtca gtaccgccag tctgaatatt gggacaaaaa ttgttcttcc
     3781 taacacggat agtcaaggga aagacagtgt ggacatgata cgtgacagct tattctctgt
     3841 tcaagtaaag caaccatggc tattgattca atatggctcc actgatgtag aagcgttagg
     3901 ggaagaacgt gtggaaagtt tattgtctac cagtccagat acaaacaatg gtgaagatag
     3961 agaaaatgta gtgattgaag aaattgaaga tcatgacaat acctatttaa ccctaccgaa
     4021 gacgatttcc agattaggaa cagtcttctt tttgtttgta ttcaatattg gtatctcgat
     4081 ttttgttttc cttctaacag gaatcatgat tttttcgcaa gtgctattta ttattttcgc
     4141 tatgtttttg ccaatcagtt tcctattaag tatgttgccg agttttgatg gcatgggcag
     4201 gcatgccatt accaagttat ttaatgtgat tatgacaagg gctggtatta ccttgattat
     4261 taccgttgcc tttagtattt ccactatgct ttattcgctg tcagcaagtt ctcccttttt
     4321 catgattatg tttttacaaa ttgtgacctt tgcagggatt tattttaaac tgggcgattt
     4381 aatgagccta ttcactttac aaagcaacga ctctcaaaac atgggcagac aagtgtttcg
     4441 cagacctcgt caaatgatga atcgacaaac cagaagattt caacgcagta tgagtaagat
     4501 ttttcgtgga aaagcagggt caacgaagca aaagacaatg acaacaaagc agaatcaaac
     4561 caataaagct aaccatactc gtcaaaatga acggactaca ccaaagaaag aaacagcact
     4621 ttccaatgtg aaaaagcaac aaggcggtat cgggcaacga ttaggtgaaa agacagccca
     4681 tgtattggac acgaaaaatc gtctagtcaa taaaagaaag caggcaaaac aacagataca
     4741 ggcgacacca acccatgtca aatatgccct tcatcaagga aaagagaatg tcactcataa
     4801 tgtaacagac tttaaacaat ctatggcaga taccaaagct caacgcaaac aacagcgtat
     4861 gaaccagcag gagcaaaaac gaaagaacat tgctcaaaag cgtttggagc tggataaagc
     4921 aaaagaaaaa agacgaaaag aaaacccatc atctcctgtt cagaataacc agcagcatgt
     4981 tcgcaagcgt cagcaaaccg tcacgcctgt tttgtcacaa aacactaaac gtgaaaatca
     5041 tcagcgacca ttgaaaaaag agcaaaaaca aaagacacca ccgaagcaat tcaatcagag
     5101 gaagctaaat aaaagaacgg ataagcgagg acaacaaaca tgaagctaaa aataacagtg
     5161 atttgtagtg tggtcttctc tctcttcttt tttctcttat tattcctagt cattttcttt
     5221 gcagatgata cggacagtgg cgaaaacaac aaggattctt ctattccaca aggcggtgtg
     5281 accgtttcac cagaagtgtt ggcacaccgt ccgttgattg aaaaatatgg taaggaatat
     5341 gggattgaag actatgtttc ttatatctta gccattatgc aagtggaatc gggcggtaca
     5401 gcagaagatg ttatgcaaag ttccgaatct ttgggtttac cgcccaacag tttgagtacc
     5461 gaagagtcca tcaaacaagg ggttaaatat ttcagtgaat tattaaccag tgcagaacaa
     5521 caaggagtgg atatagattc tgttatccaa tcttataatt atggcggtgg ctttttaaat
     5581 tatgtgagga gtcatgggaa aaaatatacc tatgaattag ccgaacagtt ttctaaagag
     5641 aagtcgggtg gtcaaaaagc agactatccc aatcctatag ccatacctgt gaatggtggt
     5701 tggcgatata actatggaaa tcagttttat gttcagttgg tttcacaata cctaaccgac
     5761 acttccccaa cagaatttga tgatgaaacc gtgcaagtca ttatggacga agccttgaaa
     5821 tacgaagggt ttccctatgt ctttggaggg gcgtcgccta ccacctcttt tgattgtagt
     5881 ggcttaatac aatgggtgta tgacaaagct ggcatttctc tccctcgtgt cgcccaagat
     5941 cagtatgacg ctacgcaaga aatctctatg gaagaagccc aagcaggcga ccttatcttc
     6001 tttcattcaa cctataatgc aggcacatat gtgactcatg tggcgatata tttagaggga
     6061 aatcgctttt atcatgcagg cgacccaatc ggttatggcg acttaagcag tcgttattgg
     6121 caagaccatc tgattggggc tagacgtgtt attcataact aagaaaggaa tgaatcaaat
     6181 gattataaaa atcgagcgaa aagaaaagaa ggagaaacca aagaaatcta aaaaaccgag
     6241 caagcaaaga aaagttccga tgattaaagt tggcacgcat aagaaattaa cctttgtttt
     6301 atgggtttta ttgattggta gtgtcggatt tggaatctat aaaaatttta ctgccattga
     6361 cactcatact gtccgtgaaa cagaaatcat taaacagcag attgtcgata ccaaccaagt
     6421 agaaagtttt gtgaaatcct ttgcgaaaga ctacttttcg tggcaacaat cccaagaagc
     6481 cattgataaa cgaaatgaaa aattgaccca ttatttgacg gaagaattgc aggtattaaa
     6541 tgaagaaatg attcgtaagg atattcctac tagttctagt gtgaacgata tacaggtgtg
     6601 gcaggtttct caagtgaatg agaacacctt tgaggtactg ttttctgttg aacaagttat
     6661 taccgaagac aaagacaagg aaactatatc gtctagcttt catgtggttg tccatataga
     6721 tgaatcagat aatatggtta ttatcaaaaa tccgacaatg agtaagaaac ctcaaaaatc
     6781 ggactaccaa cccaaacagc tagaaagtga ccatacagta gatacagaaa cgatggacga
     6841 aatcactagc tttttagaaa cgttcttcca gctctatcct accgccacag aaaaagaatt
     6901 gacttactat gtaagtaatc atgtgttacc tatgattaac aaggaatatg ttttcgagga
     6961 attggtgaat cctatcttta ccagaaaaga taaccaagta atcgtgaatg tcgctgtgaa
     7021 atatttagat caagaaacaa aggcaaccca aatctcgcaa tttgagttga tattagaaaa
     7081 acaagataat tggaagattg taaaataaca aatattggta catgattaca gatactttgt
     7141 aatcatgtac tctttttgat aaaaaattgg agattccttt acaaatatgc tcttacgtgc
     7201 tattatttaa gtgactattt aaaaggagtt aataaatatg cggcaaggta ttcttaaata
     7261 aactgtcaat ttgatagcgg gaacaaataa ttagatgtcc ttttttagga gggcttagtt
     7321 ttttgtaccc agtttaagaa tacctttatc atgtgattct aaagtatcca gagaatatct
     7381 gtatgctttg tatacctatg gttatgcata aaaatcccag tgataaaagt atttatcact
     7441 gggattttta tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa attattaata
     7501 ttggagtttt agctcatgtt gatgcaggaa aaactacctt aacagaaagc ttattatata
     7561 acagtggagc gattacagaa ttaggaagcg tggacaaagg tacaacgagg acggataata
     7621 cgcttttaga acgtcagaga ggaattacaa ttcagacagg aataacctct tttcagtggg
     7681 aaaatacgaa ggtgaacatc atagacacgc caggacatat ggatttctta gcagaagtat
     7741 atcgttcatt atcagtttta gatggggcaa ttctactgat ttctgcaaaa gatggcgtac
     7801 aagcacaaac tcgtatatta tttcatgcac ttaggaaaat ggggattccc acaatctttt
     7861 ttatcaataa gattgaccaa aatggaattg atttatcaac ggtttatcag gatattaaag
     7921 agaaactttc tgccgaaatt gtaatcaaac agaaggtaga actgtatcct aatatgtgtg
     7981 tgacgaactt taccgaatct gaacaatggg atacggtaat agagggaaac gatgaccttt
     8041 tagagaaata tatgtccggt aaatcattag aagcattgga actcgaacaa gaggaaagca
     8101 taagatttca gaattgttct ctgttccctc tttatcatgg aagtgcaaaa agtaatatag
     8161 ggattgataa ccttatagaa gttattacta ataaatttta ttcatcaaca catcgaggtc
     8221 cgtctgaact ttgcggaaat gttttcaaaa ttgaatatac aaaaaaaaga caacgtcttg
     8281 catattaagt agttcagttt tggagtacaa aaagtgaaaa tgtaaaccta aatgagtatc
     8341 aaattgcaac tgaatttgat actcgttttt tattgttaaa tcaacgtttg ttgttaattt
     8401 acaccacaaa atgtaagtta aaatgtatat aaaaatgcga acgtgaaata attattgtag
     8461 gaatgcaaat tggtgtgtta aaatggttac aatttactaa gcaaaaaggg gtgcattttc
     8521 ctatgcaatt tcttgagcca aaaaataaga acgctaagtc tgtggattgg gaaatatcag
     8581 agcaagtaag ggtgatagtt aagcaatacg ctgaatatgc agagcgtact gaaagcgaag
     8641 cagtggatga attcttattg aacattttag acgataagaa gtttattgaa tggattgcca
     8701 acaagcgaag taataagcgg attgttgaaa aaatgggtat caaagataga gtgggttgat
     8761 agacaatgac caagcttaaa agattagcca cgaaggaaga tgaaattgta gatgttaaaa
     8821 ttccgatttc aaacgaagaa ttaaaagata gggctaaaca atatgattta ttaactccaa
     8881 aacgatttgc aacaaggtat aacaaaatgt tgttcttgcc aacgtctttt aaatggaacg
     8941 gttctgaata tcccattcaa tataattact gcatcaatcc gttttgttgt aattttggga
     9001 aagaacagca caaattcaaa gatgtaaaag ggaaaccaag tcgatataaa atgactggta
     9061 gttccaaaga taaaggtcat aaaggtatgt actgcaatga caatcctatt ggcagaggtg
     9121 tatcgcaaaa ctgtacagta acccctttat ctaattggtc tgttgttgaa gaaattaaac
     9181 gccttataga aatcaatagc atccaagatg ttgagccaga ttatcaattt cataaagaag
     9241 gctgctcgga agaagaatca accccattca atgagccgaa gcaattttac aaaaggggga
     9301 aaagtagggg caaatctcaa cgctatcaat gtaaggcgtg taagaagttc actaatgttc
     9361 taccaaaaag agaagaaacc actacttatc accaacaaaa gaacaccatt cttccaatgt
     9421 tcgctaagat ggttgttgga agggtttcag tcagtcgtac ttgtgatatt ttaggaattg
     9481 gagttggaac ctactatcat aagctggagt ggctgtatag gcgttgtttg gagtttttag
     9541 aacgatatga gacacaaccc cttcaaacaa agaaatttaa cgagatgtgg cttaatacgg
     9601 acaagatgca ctactaccta aataacgtcc gtaagaaagg acaaggttca aagaaatata
     9661 cggggtttga agaccttaat atgcaaacgt atatagttgt atctgctgag gtcttatcaa
     9721 gatatgtgtt ccgttctgat gttgcctatg attggaatat ttcaatggat gaattaaatg
     9781 aagatacaag aaaattcaaa gaagaccatc tcaatacttt tagtcgtaaa aatgataggt
     9841 tagattggtc ttattatcca caagaaccat ccgctaacga tagcgaaaat aggaatgctt
     9901 atcttcacga attagggaaa attactaata ggagtagatt tgtcgatggt ttaaatgttg
     9961 atgctcctta tactacaact gctcactatt ggttaattaa acaaatggtt aatgccgacg
    10021 aatggcgaat gattagtgat gatgacttct ctattagaaa cgccttttat agagtattca
    10081 cgaaagaatt aaggttgagc gatgcacatc attttatttg ccaagttaac aagacgaaaa
    10141 gcagaaaaca atgcttaaaa gaattcggac aagcaaaagc agaactgctc gattggggag
    10201 atatcagagg attcaaaaca aagtttctac ggactttggc atctcattat ctaactgaat
    10261 tactaacaag tcatcaattc cacgaagagg ctattagtaa agatggagaa agatatcgca
    10321 aatatgccga taaccctatc aaacatccat tagccactaa ggataaagga ttttattcag
    10381 ttgattgtag aacggattta tctgctctgg aaccaaacga aatcgcaaag atgctattaa
    10441 atgttaatga ccattcgacc aatagcttta ttcaacagat aagacgttat atatcatctc
    10501 ttgaaagacc attaacaaca gctcgtggtg ataagaagag ctatatttac gctaacttca
    10561 atccgaagta tgctcaattt gcgattacaa ttctaagaac ttactacaac ttttgtcgcc
    10621 cttttaaatc agcggataaa aaggttttga cacctgctca aagattaggg attacagata
    10681 aacaatttga ttggaaagat ataatttatt tcaaatgaca aaaagagctt ggagtaatcc
    10741 caagctcttg tgctttatct ttactgttct tcttcaatta acgcacccgt taactcaata
    10801 agttttttct ttcatataca tgaaaatagt agttatacgg atttttatca ttctttatcc
    10861 ctttttgggc aaatacctca ttccattcct cataattcac ttctggaaaa aaagtatctc
    10921 cttcgaattc atgatgtatt tttgtgatgt acattttctc aacataaggg aaaaacaaat
    10981 tataaatctg ttctcctccg aaaataaaaa tttcttcttc gtttttacat aactcaaaaa
    11041 catcttctat tgaatgaaca atttcacaac cattaaaggt aaacccctta tctctcgtca
    11101 gaataatatt tcttctgtca ggtaaggctc ttccgattga ttcaaggttc ttcctaccta
    11161 atattatcgg atgtcccttt gtagtatttt taacatattc ccagtccttg ggaatcctcc
    11221 aaggaatgtc attctctttg ccaatcactc tattcttatc catcgcagca atcaaagaaa
    11281 ctttcattta taacacagcc tttttaacta atttaccatc taatttctta tttctatcaa
    11341 aaaacctttt ctcattatcc tgcccgttag ttgaataagc aattttttct ataagttata
    11401 ttatcataat accacaaaat cacaaatttt cattcaatta tcattttcaa ttgcatttta
    11461 taaatcattt tgtatgtaat ttggtatgta agttaaaatg aaaaaagtag tccattttga
    11521 agtacatttt gtcattttgt actccaaaac tgaactactt acttgcatat atacgccttt
    11581 atagtggagt actacattta cgagattcgg ttagagtatc agaaaaagaa aaaataaaag
    11641 ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg
    11701 gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa
    11761 aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg
    11821 aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata
    11881 gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct
    11941 tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga
    12001 tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata
    12061 ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac
    12121 cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc
    12181 aatcatttca aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa ggattatatg
    12241 gttggaatgt gacggattgt aaaatctgtt ttaagtacgg tttatactat agccctgtta
    12301 gtactccagc agattttcgg atgcttactc ctattgtact ggagcaagcc tttagaaaag
    12361 ctggaacaga attgttagag ccatatctta gttttaaagt ttatgcacca caggaatatc
    12421 tttcacgggc atataacgat gctcccaaat attgtgcaaa tatcgtaaat actcaactga
    12481 aaaataatga ggtcattatt attggagaaa ttcctgctcg atgtattcaa gattatcgca
    12541 atgatttaac tttttttaca aatgggctta gtgtttgttt agcagagcta aaaggatatc
    12601 aggttaccac tggcgaacct gtttgccaga cccgtcgtct aaatagtcgg atagataaag
    12661 taagatatat gttcaataaa ataacttagt gcgttttatg ttgttatata aatatggttt
    12721 cttattaaat aagatgaaat attctttaat atagatttga attaaagtgg aaaggaggag
    12781 attgttatta taaactacaa gtggatattg tgtcctattt gtggaaataa aacaagacta
    12841 cgaatacgag tggatactat acttaaaaat ttccctttat actgccccaa atgtaagaac
    12901 gaaactttaa ttaatgttca aaaaatgaat ataataacaa tcaaagagcc agacgccaag
    12961 acgcagagcc gataatttga gaaatgaaac tctcatctta tcggctcttt ttgtttatct
    13021 gaattttact gactagcctt caatatttct tgcttcattg ataccttttg ctag
//
LOCUS       DAYYDS010000271        11846 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.guided.081, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000271 DAYYDS010000000
VERSION     DAYYDS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.guided.081"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACHA02_003054"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACHA02_003054"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8671290.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACHA02_003055"
     CDS             complement(2159..3031)
                     /locus_tag="ACHA02_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8671291.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACHA02_003056"
     CDS             complement(3373..4245)
                     /locus_tag="ACHA02_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8671292.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACHA02_003057"
     CDS             4373..4942
                     /locus_tag="ACHA02_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8671293.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACHA02_003058"
     CDS             5009..5746
                     /locus_tag="ACHA02_003058"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8671294.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACHA02_003059"
     CDS             5977..7245
                     /locus_tag="ACHA02_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8671295.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACHA02_003060"
     CDS             7732..8196
                     /locus_tag="ACHA02_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8671296.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACHA02_003061"
     CDS             8180..8809
                     /locus_tag="ACHA02_003061"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8671297.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACHA02_003062"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACHA02_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8671298.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACHA02_003063"
     CDS             10688..11134
                     /locus_tag="ACHA02_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8671299.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..>11846)
                     /locus_tag="ACHA02_003064"
     CDS             complement(11333..>11846)
                     /locus_tag="ACHA02_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8671300.1"
                     /translation="NTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKLD
                     QQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLDK
                     TTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQQ
                     VREQMQQFFE"
BASE COUNT         3757 a         2199 c         2390 g         3500 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattc
//
LOCUS       DAYYDS010000272         8068 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.guided.111, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000272 DAYYDS010000000
VERSION     DAYYDS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.guided.111"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACHA02_003065"
     CDS             complement(134..577)
                     /locus_tag="ACHA02_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8671301.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACHA02_003066"
     CDS             complement(795..2135)
                     /locus_tag="ACHA02_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8671302.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACHA02_003067"
     CDS             2404..3300
                     /locus_tag="ACHA02_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8671303.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACHA02_003068"
     CDS             complement(3450..4391)
                     /locus_tag="ACHA02_003068"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8671304.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACHA02_003069"
     CDS             4517..5638
                     /locus_tag="ACHA02_003069"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8671305.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACHA02_003070"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACHA02_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8671306.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACHA02_003071"
     CDS             7465..7992
                     /locus_tag="ACHA02_003071"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8671307.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2673 a         1506 c         1598 g         2291 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgac
//
LOCUS       DAYYDS010000273         8061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.guided.112, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000273 DAYYDS010000000
VERSION     DAYYDS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.guided.112"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACHA02_003072"
     CDS             128..1564
                     /locus_tag="ACHA02_003072"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8671308.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACHA02_003073"
     CDS             1753..4200
                     /locus_tag="ACHA02_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8671309.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACHA02_003074"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACHA02_003074"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8671310.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACHA02_003075"
     CDS             5487..5693
                     /locus_tag="ACHA02_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671311.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACHA02_003076"
     CDS             complement(5690..6793)
                     /locus_tag="ACHA02_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8671312.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACHA02_003077"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACHA02_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8671313.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2696 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DAYYDS010000274         5613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.guided.136, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000274 DAYYDS010000000
VERSION     DAYYDS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.guided.136"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..1026)
                     /locus_tag="ACHA02_003078"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="ACHA02_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR-A"
                     /locus_tag="ACHA02_003079"
     CDS             1178..1873
                     /gene="vanR-A"
                     /locus_tag="ACHA02_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFM8671314.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="ACHA02_003080"
     CDS             1851..3005
                     /locus_tag="ACHA02_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFM8671315.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="ACHA02_003081"
     CDS             3220..4188
                     /locus_tag="ACHA02_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM8671316.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="ACHA02_003082"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="ACHA02_003082"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM8671317.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="ACHA02_003083"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="ACHA02_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DAYYDS010000275         2128 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.guided.188, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000275 DAYYDS010000000
VERSION     DAYYDS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.guided.188"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..345
                     /locus_tag="ACHA02_003084"
     CDS             <1..345
                     /locus_tag="ACHA02_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8671318.1"
                     /translation="ELPVVANLNLKFTTKKFSLYPEKFFLVKDLWIAVMQNSSNKLPK
                     TYNHIAFKIDEQDIDSFVSKIQMLGLTVEPGRSRVKGEASSIYFYDYDNHLFELHTGT
                     LQERLKSYNSTT"
     gene            complement(617..907)
                     /locus_tag="ACHA02_003085"
     CDS             complement(617..907)
                     /locus_tag="ACHA02_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8671319.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1115..1807)
                     /locus_tag="ACHA02_003086"
     CDS             complement(1115..1807)
                     /locus_tag="ACHA02_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8671320.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          598 a          397 c          319 g          814 t
ORIGIN      
        1 gagttacctg tagtggctaa cttaaacttg aaatttacga caaaaaaatt ttcattatat
       61 ccagaaaaat ttttcttagt taaggaccta tggattgcag taatgcagaa ttcgtcgaat
      121 aaactgccaa agacatacaa tcatattgct tttaaaattg acgaacaaga tatagattcg
      181 tttgtctcaa aaatccaaat gttaggttta actgtcgaac caggacgttc tcgtgtgaaa
      241 ggcgaggctt cttcaattta cttttacgac tatgataatc acctcttcga gttacatacc
      301 ggtactctcc aggaacgttt aaaatcctac aattccacta cctgagacta gtaatgacta
      361 gtctcttttt atggcctctg atccccccat tgagcgaata ctaatagggc ttcgaattga
      421 tctgttcaat ctttaagcaa tatccctcaa atgagtttgg aaagtttagt gattcatcac
      481 aaatacttaa atacatgtag ttatatatag attgaggttc ttgtgagcgt atgctcgtgt
      541 gggttcaaat cccatcgtct gcatccatct ttccaaaaca agcggctagg acatttttgt
      601 ctcagccgct tgttttctag tttattcttt catcttgtcg gacctttttt cttgggtata
      661 gacttttctc atattctacc acaattttgg gtttccctaa cacatgatag gatctcacaa
      721 caaattgctt tctattatta taataacctg taacgactaa cttcatccct tcgtctactt
      781 cagctaggaa gttcaaacta tgggaagcaa tcaaacaatt gaccccattt aaactgaaac
      841 ggatcaacgg tctggcacta acttgcaaag tacggatctt atctactgtt ccatattttt
      901 tctccataac cttcacctca ataccattat acgaacatac attccctttg taaagaagaa
      961 actctgtttt atctttttga ctttttgtct attacctttc ttaaaatcat attggcactt
     1021 cctgcgaaga tgaaggacat gtactaaaaa aaagaggcag cagtatagat tactgttgct
     1081 tctttactaa tttaggttta cttgttaaga aagattattt acgcaaatgt tcttcaacaa
     1141 tttgctgaga tttatcagct gtatcttcca cttcatcttt tgctttttct aacttttgaa
     1201 tatattgttt caagcttgag ttttcagcag ttgcttcgtc taatttcttt tgtgtatctg
     1261 ttaactgagt ttctacattt gttttttctt gtgtcagtgt tgcgatttgt tgattcaaac
     1321 catttacagt attttgtaat tctgaaagtt ttgcttgtgc ttcttgtagt cttgctgtcc
     1381 aatctgcatt cagatgattg atttcatcat ttttttggtt gatctcatct agtttgacat
     1441 tgatttcatt ttgtttgctt tgtagttgtg catccttgct gactaattga ctttctaagt
     1501 tttgtttttc tgctgccgat tgattttttt ggttttctaa ttcatttact tttgtttggt
     1561 attcttggac atctgccttt gaatcttgca atgtctgttc tttttgtgct agtcgagcgg
     1621 taagatctgt aagcgcttgc tctttatctg tcaatgtatg atcaagctgt gtcagattct
     1681 cattgattgt atcaatatct gtctctccac cccacaaaat aatttcattt gcgtaccgcg
     1741 tgcctgtgta gccaattaga catagaaatg caacgacaat cccaccagta ataaatttct
     1801 ttttcatttg catagctcct taatccaata cttttacatg ctaaaagtaa gtgatctcaa
     1861 acacaatgtc aagcgttttt aaattacatg tgtaatttta atataaatac tatcaaatac
     1921 tcatttaatc ctcctttttt atctatttaa aaaatctttt ttatagagaa aaatgcaggt
     1981 taatactttc ttaaataaat atttatggtg aacaaaaaca aaaaagcaag ttttattaaa
     2041 cgtaaccctt ttcatttcat agattactgt atttattaca aaaaacgcgg atttgtcata
     2101 aattgtcatg cttcttttac ctatgaaa
//
LOCUS       DAYYDS010000276         1781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.guided.200, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000276 DAYYDS010000000
VERSION     DAYYDS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.guided.200"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..416
                     /locus_tag="ACHA02_003087"
     CDS             24..416
                     /locus_tag="ACHA02_003087"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8671321.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ACHA02_003088"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ACHA02_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFM8671322.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DAYYDS010000277         1504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.guided.209, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000277 DAYYDS010000000
VERSION     DAYYDS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.guided.209"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACHA02_003089"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACHA02_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACHA02_003090"
     CDS             394..477
                     /locus_tag="ACHA02_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFM8671323.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHA02_003091"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHA02_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8671324.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAYYDS010000278         1382 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.guided.215, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000278 DAYYDS010000000
VERSION     DAYYDS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.guided.215"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1311
                     /locus_tag="ACHA02_003092"
     CDS             <1..1311
                     /locus_tag="ACHA02_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFM8671325.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DAYYDS010000279         1350 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.guided.218, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000279 DAYYDS010000000
VERSION     DAYYDS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.guided.218"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..298
                     /locus_tag="ACHA02_003093"
                     /pseudo
     CDS             47..298
                     /locus_tag="ACHA02_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ACHA02_003094"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ACHA02_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8671326.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAYYDS010000280         1194 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4688
            SAMEA115707250-rid22595463.guided.229, whole genome shotgun
            sequence.
ACCESSION   DAYYDS010000280 DAYYDS010000000
VERSION     DAYYDS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707250
            Sequence Read Archive: ERR13196981
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290062.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 15:58:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,030
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 53 of 95
            Pseudo Genes (internal stop)      :: 26 of 95
            Pseudo Genes (multiple problems)  :: 12 of 95
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707250-rid22595463.guided.229"
                     /isolate="VRE4688"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="ACHA02_003095"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="ACHA02_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFM8671327.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
